Application of RSPH9 as diagnosis marker or treatment target for oligoasthenozoospermia

A technology for asthenozoospermia and oligospermia, which is applied in the field of preparation of diagnostic reagents or medicines for treating oligoasthenospermia, can solve problems such as the lack of effective treatment drugs, and achieve the effects of improving accuracy and reducing the probability of misdiagnosis

Pending Publication Date: 2020-08-11
山东立菲生物产业有限公司
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Problems solved by technology

The clinical treatment is mainly based on traditional Chinese medicine or the combination of traditional Chinese and Western medicine, and the medicine for invigorating kidney essence, qi and blood deficiency is used for treatment, and there is no effective treatment medicine yet

Method used

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  • Application of RSPH9 as diagnosis marker or treatment target for oligoasthenozoospermia
  • Application of RSPH9 as diagnosis marker or treatment target for oligoasthenozoospermia
  • Application of RSPH9 as diagnosis marker or treatment target for oligoasthenozoospermia

Examples

Experimental program
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Embodiment 2

[0049] Embodiment 2 Clinical detection verification

[0050] According to the method of Example 1, protein extraction and nano-flow liquid chromatography-tandem mass spectrometry were performed on the collected sperm of 10 groups of patients with oligoasthenospermia, and then the mass spectrometry data were analyzed. Table 1 shows the analysis results, oligoasthenospermia was detected in 10 people using the above method. The accuracy rate of this group of data is as high as 100%.

[0051] Table 1

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Abstract

The invention particularly relates to an application of RSPH9 as a diagnosis marker or a treatment target for oligoasthenozoospermia. Sperm proteins of normal and patients are extracted, bovine serumalbumin is used as a standard substance, and multiple groups of sperm proteins of severe application of RSPH9 as a diagnosis marker or a treatment target for oligoasthenozoospermia diseases are subjected to deep mass spectrometry by using high-resolution biomass spectrometry and a non-labeled quantitative proteomics method; the mass spectrum data are searched by using a protein quantitative analysis method, and the protein in the sperm is identified; and finally, normal and patient sperm proteomes are compared, and screening is conducted to obtain that the contents of RSPH9 in healthy and oligoasthenosperm samples are remarkably different. ROC analysis further verifies that RSPH9 has good specificity as a diagnostic marker, and is expected to be used as a diagnostic marker and a treatmenttarget for severe oligoasthenozoospermia.

Description

technical field [0001] The invention belongs to the technical field of diagnosis and treatment of oligoasthenospermia, and specifically relates to the application of RSPH9 as a diagnostic marker or therapeutic target for oligoasthenospermia in the preparation of diagnostic reagents or drugs for the treatment of oligoasthenospermia. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] According to the survey results of WHO, 15% of couples of childbearing age have infertility problems, and infertility has become a global medical and social problem affecting human health and social development (Turner, T.T. and J.J. Lysiak, Oxidative stress: a common factor in testicular dysfunct...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68A61K45/00A61P15/08
CPCA61K45/00A61P15/08G01N33/6893G01N2333/47G01N2800/367
Inventor 黄宁张亚卓林克萌孙胜楠董锡阳
Owner 山东立菲生物产业有限公司
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