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11 results about "Quantitative proteomics" patented technology
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Quantitative proteomics is an analytical chemistry technique for determining the amount of proteins in a sample. The methods for protein identification are identical to those used in general (i.e. qualitative) proteomics, but include quantification as an additional dimension. Rather than just providing lists of proteins identified in a certain sample, quantitative proteomics yields information about the physiological differences between two biological samples. For example, this approach can be used to compare samples from healthy and diseased patients. Quantitative proteomics is mainly performed by two-dimensional gel electrophoresis (2-DE) or mass spectrometry (MS). However, a recent developed method of quantitative dot blot (QDB) analysis is able to measure both the absolute and relative quantity of an individual proteins in the sample in high throughput format, thus open a new direction for proteomic research. In contrast to 2-DE, which requires MS for the downstream protein identification, MS technology can identify and quantify the changes.
Cloning and application of rice bacterial blight resistant gene LTPL160
The invention relates to a DNA (Deoxyribose Nucleic Acid) for regulating and controlling a resistance gene LTPL160 of rice bacterial blight and breeding utilization of a protein of the DNA. According to the invention, the gene OsLTPL160 for regulating and controlling the resistance of the rice bacterial leaf blight is cloned by virtue of a non-gel quantitative proteomics analysis and transcription analysis method. The OsLTPL160 knockout and overexpression material is obtained by adopting a gene editing and transgenic technology. The OsLTPL160 is knocked out, so that the bacterial blight resistance of the rice is remarkably improved; and overexpression of the OsLTPL160 obviously reduces the resistance of the bacterial blight of the rice. The cloning of the OsLTPL160 gene and the construction of the material provide material and technical support for the bacterial blight resistance of the rice.
Owner:JIANGSU ACAD OF AGRI SCI +1
Marker combination, kit thereof and application of marker combination in prediction of diabetic peripheral neuropathy
The invention discloses a marker combination, a kit thereof and application of the marker combination to prediction of diabetic peripheral neuropathy, the marker combination comprises human angiotensin (ANG), human vascular endothelial cell adhesion molecule 1 (VCAM1) and human mannan binding lectin serine peptide 1 (MASP1), TMT quantitative proteomics and PRM targeted proteomics are adopted, and the marker combination is used for detecting diabetic peripheral neuropathy. A technology combination strategy of an ELISA experiment is adopted, and key differential expression proteins such as MASP1, VCAM1 and ANG in serum exosomes of patients with diabetic peripheral neuropathy are systematically screened and verified. By establishing a multi-model machine learning diagnosis system based on logistic regression, a support vector machine and a naive Bayes algorithm, simple and rapid identification of diabetic peripheral neuropathy patients is realized, and a new experimental basis is provided for clarification of a molecular regulation network of diabetic peripheral neuropathy; more importantly, a rapid screening tool with clinical application potential is developed.
Owner:SHANGHAI TENTH PEOPLES HOSPITAL
Single cell level proteomics analysis system and analysis method for mass spectrum system
PendingCN121431702AComponent separationBiological testingEnzymatic digestionMass Spectrometry-Mass Spectrometry
The invention belongs to a proteomics analysis technology in the technical field of biology, and particularly relates to a single-cell-level proteomics analysis system and analysis method for a mass spectrum system. The analysis system comprises a sample pretreatment optimization subsystem and a chromatography-mass spectrometry acquisition optimization subsystem, the purpose of the sample pretreatment optimization subsystem is to efficiently and stably prepare a peptide fragment mixture capable of being used on a mass spectrum machine from a trace cell sample. The sample loss is reduced by improving the protein extraction and enzymolysis efficiency of trace (nanogram-level) and single-cell-level protein samples. By optimizing mass spectrum data acquisition parameters, the identification depth and quantity of proteins are improved while the analysis time is shortened (the flux is improved). By adopting the analysis system disclosed by the invention, the accuracy and reproducibility of quantitative analysis of the micro sample proteome can be remarkably improved.
Owner:ZHEJIANG UNIV OF TECH +1
A method for screening a shikonin acting target point based on LIP-MS technology
ActiveCN119395303Bimprove accuracyhigh sensitivityComponent separationBiological testingPeptide FingerprintsProtein target
The application discloses a method for screening shikonin acting target points based on LIP-MS technology, and relates to the technical field of medicines, and has the technical features that the application adopts a widely specific protease to perform limited enzymolysis on a protein sample, utilizes the characteristic that drug and target protein combination can improve the conformation stability of the combination region peptide segment, further uses trypsin to perform secondary enzymolysis, collects a peptide fingerprint by using quantitative proteomics technology, analyzes and compares the changes of the peptide fingerprints of a control group and a drug treatment group, screens out the specificity of the 'conformational retention peptide segment' after drug treatment, identifies the target protein information corresponding to the peptide segment by database matching, compared with the traditional DARTS method, the non-labeled drug target analysis based on the peptide fingerprint changes the detection object from the protein into the peptide segment, and is expected to characterize the low-affinity multi-target drug and protein interaction, low-abundance protein, large molecular weight protein local conformation change information, so as to improve the accuracy and sensitivity of target analysis.
Owner:THE NAVAL MEDICAL UNIV OF PLA
Drug target of tribulus terrestris extract for treating pulmonary fibrosis as well as screening method and application of drug target
PendingCN120733072ACompounds screening/testingMicrobiological testing/measurementProtein targetPhosphorylation
The invention relates to the technical field of pharmacological research, in particular to a drug target of tribulus terrestris extract for treating pulmonary fibrosis as well as a screening method and application of the drug target. According to the invention, the effective treatment effect of TED on PF is determined through pharmacodynamic research. The SCD2 protein is preliminarily screened as the target protein by using a 4D-SmartDIA quantitative proteomics technology, the protein expression level proves that the SCD2 expression and phosphorylation level in the mouse lung tissue after TED treatment are obviously increased, and the interaction between SCD2 and phosphorylation is investigated and determined. Therefore, the SCD2 can be used as a drug target for predicting the treatment of the TED on the PF. And a basis is provided for development of PF treatment drugs.
Owner:BAOTOU MEDICAL COLLEGE OF INNER MONGOLIA UNIV OF SCI & TECH
Method for detecting protein having changes in energy state, or affinity of ligand to protein
Disclosed in the present invention is a method for detecting a protein having changes in an energy state, and affinity of a ligand to a protein. Specifically, after the energy state of a protein changes, the tolerance to enzyme cleavage destruction changes, the structure of the protein in a low-energy state is also destroyed under a non-denaturation condition by using a large amount of enzymes, and a small peptide fragment which has a molecular weight of less than 5 KDa and can be directly used for bottom-top mass spectrometry analysis is directly generated. The method has extremely high sensitivity, and quantitative proteomics is used to find enzyme cleavage differential peptide fragments, and proteins to which the differential peptide fragments belong and the positions in the proteins are analyzed, so that a protein having changes in an energy state, and a change region can be determined in the whole proteome range. If the energy state of the protein changes due to addition of a ligand, the method can determine a binding protein and a binding region of the ligand; and the output of a quantitative result on the peptide fragment level further enables the method to determine the local affinity of binding of the ligand to the protein.
Owner:DALIAN INSTITUTE OF CHEMICAL PHYSICS CHINESE ACADEMY OF SCIENCES
18 beta-glycyrrhetinic acid derivative containing diene structure fragment as well as preparation method and application of 18 beta-glycyrrhetinic acid derivative
The invention discloses a 18 beta-glycyrrhetinic acid derivative containing a diene structure fragment as shown in a general formula I and a preparation method and application thereof, and belongs to the technical field of medicines, the compound can effectively degrade HDAC3, and further mechanism studies (including Western Blot and quantitative proteomics) prove that a representative compound can effectively degrade the HDAC3 by inducing the degradation of the HDAC3 through specificity. Further, an NLRP3 inflammasome pathway is inhibited, and finally, maturation and secretion of downstream inflammatory factors IL-1beta and caspase-1 are blocked, so that a remarkable anti-inflammatory effect is achieved. A mouse acute shock and colitis model further verifies the excellent anti-inflammatory activity of the compound in vivo. The invention not only provides a novel HDAC3 degradation agent with good activity, but also lays a solid material and theoretical foundation for deep development of anti-inflammatory drugs based on the 18 beta-glycyrrhetinic acid structure.
Owner:SHENYANG PHARMA UNIV
Use of a combination of plasma proteins in screening for pancreatic cancer
PendingCN122109536AMedical data miningComponent separationPancreas CancersMass Spectrometry-Mass Spectrometry
The application belongs to the technical field of proteomics and clinical disease screening, and relates to application of a combination of plasma proteins in screening of pancreatic cancer. The application is based on mass spectrometry quantitative proteomics technology, screens and determines a marker combination composed of four plasma proteins of GCLC, ALAD, HAGH and SORD, and comprehensively analyzes relative abundance of the marker combination through a binary Logistic regression model to distinguish pancreatic cancer patients from healthy people. Experimental results show that the protein combination has high sensitivity, specificity and area under curve (AUC) in a screening set and an independent verification set, and has good stability and repeatability. The method has the advantages of non-invasiveness, feasible operation and applicability to population screening by taking plasma as a detection sample, and can provide a new technical means for early screening of pancreatic cancer.
Owner:ACADEMY OF MILITARY MEDICAL SCIENCES +2
Digital protein quantification
ActiveUS12467931B2Sequential/parallel process reactionsLibrary tagsBioinformaticsQuantitative proteomics
Methods and compositions are described for single cell resolution, quantitative proteomic analysis using high throughput sequencing.
Owner:BIO RAD LABORATORIES INC +1
Comprehensive evaluation method for efficacy quality of traditional Chinese medicine compound and application of comprehensive evaluation method
The invention provides a traditional Chinese medicine compound efficacy quality comprehensive evaluation method and application thereof, the comprehensive evaluation method adopts a targeted quantitative proteomics technology to quantify an anti-myocardial ischemia biomarker of a Guanxintong capsule, and quantitative analysis is carried out on the anti-myocardial ischemia biological effect of the Guanxintong capsule from the cellular level. According to the invention, the accurate quantitative correlation between the Q-marker of the Guanxinshutong capsule and the anti-myocardial ischemia biomarker based on the BP neural network is constructed, the overall quality evaluation system of the Guanxinshutong capsule based on the Q-marker is established, the quantitative correlation with the overall biological effect is realized, the quality standard of a high-quality product is established, and a reference is provided for the quality and efficacy evaluation of a traditional Chinese medicine compound.
Owner:SHANXI BUCHANG PHARMA CO LTD
Low energy cleavable mass tag for quantitative proteomics
The present invention relates to a compound which comprises or consists of (a) a reactive moiety, said reactive moiety being capable of reacting with a functional group of a peptide to form a covalent bond; and covalently joined thereto (b) a moiety which fragments in the mass spectrometer (i) at an energy below the energy required for fragmenting peptides and / or a higher conversion rate than peptides; and (ii) at said energy according to (i) and when coupled to a peptide via said reactive group, at a single site within said compound coupled to a peptide, to yield a first moiety and a second moiety, said second moiety being coupled to said peptide.
Owner:BRUKER SWITZERLAND AG