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5 results about "Quantitative proteomics" patented technology
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Quantitative proteomics is an analytical chemistry technique for determining the amount of proteins in a sample. The methods for protein identification are identical to those used in general (i.e. qualitative) proteomics, but include quantification as an additional dimension. Rather than just providing lists of proteins identified in a certain sample, quantitative proteomics yields information about the physiological differences between two biological samples. For example, this approach can be used to compare samples from healthy and diseased patients. Quantitative proteomics is mainly performed by two-dimensional gel electrophoresis (2-DE) or mass spectrometry (MS). However, a recent developed method of quantitative dot blot (QDB) analysis is able to measure both the absolute and relative quantity of an individual proteins in the sample in high throughput format, thus open a new direction for proteomic research. In contrast to 2-DE, which requires MS for the downstream protein identification, MS technology can identify and quantify the changes.
Cloning and application of rice bacterial blight resistant gene LTPL160
The invention relates to a DNA (Deoxyribose Nucleic Acid) for regulating and controlling a resistance gene LTPL160 of rice bacterial blight and breeding utilization of a protein of the DNA. According to the invention, the gene OsLTPL160 for regulating and controlling the resistance of the rice bacterial leaf blight is cloned by virtue of a non-gel quantitative proteomics analysis and transcription analysis method. The OsLTPL160 knockout and overexpression material is obtained by adopting a gene editing and transgenic technology. The OsLTPL160 is knocked out, so that the bacterial blight resistance of the rice is remarkably improved; and overexpression of the OsLTPL160 obviously reduces the resistance of the bacterial blight of the rice. The cloning of the OsLTPL160 gene and the construction of the material provide material and technical support for the bacterial blight resistance of the rice.
Owner:JIANGSU ACAD OF AGRI SCI +1
Single cell level proteomics analysis system and analysis method for mass spectrum system
PendingCN121431702AComponent separationBiological testingEnzymatic digestionMass Spectrometry-Mass Spectrometry
The invention belongs to a proteomics analysis technology in the technical field of biology, and particularly relates to a single-cell-level proteomics analysis system and analysis method for a mass spectrum system. The analysis system comprises a sample pretreatment optimization subsystem and a chromatography-mass spectrometry acquisition optimization subsystem, the purpose of the sample pretreatment optimization subsystem is to efficiently and stably prepare a peptide fragment mixture capable of being used on a mass spectrum machine from a trace cell sample. The sample loss is reduced by improving the protein extraction and enzymolysis efficiency of trace (nanogram-level) and single-cell-level protein samples. By optimizing mass spectrum data acquisition parameters, the identification depth and quantity of proteins are improved while the analysis time is shortened (the flux is improved). By adopting the analysis system disclosed by the invention, the accuracy and reproducibility of quantitative analysis of the micro sample proteome can be remarkably improved.
Owner:ZHEJIANG UNIV OF TECH +1
A method for screening a shikonin acting target point based on LIP-MS technology
ActiveCN119395303Bimprove accuracyhigh sensitivityComponent separationBiological testingPeptide FingerprintsProtein target
The application discloses a method for screening shikonin acting target points based on LIP-MS technology, and relates to the technical field of medicines, and has the technical features that the application adopts a widely specific protease to perform limited enzymolysis on a protein sample, utilizes the characteristic that drug and target protein combination can improve the conformation stability of the combination region peptide segment, further uses trypsin to perform secondary enzymolysis, collects a peptide fingerprint by using quantitative proteomics technology, analyzes and compares the changes of the peptide fingerprints of a control group and a drug treatment group, screens out the specificity of the 'conformational retention peptide segment' after drug treatment, identifies the target protein information corresponding to the peptide segment by database matching, compared with the traditional DARTS method, the non-labeled drug target analysis based on the peptide fingerprint changes the detection object from the protein into the peptide segment, and is expected to characterize the low-affinity multi-target drug and protein interaction, low-abundance protein, large molecular weight protein local conformation change information, so as to improve the accuracy and sensitivity of target analysis.
Owner:THE NAVAL MEDICAL UNIV OF PLA
18 beta-glycyrrhetinic acid derivative containing diene structure fragment as well as preparation method and application of 18 beta-glycyrrhetinic acid derivative
The invention discloses a 18 beta-glycyrrhetinic acid derivative containing a diene structure fragment as shown in a general formula I and a preparation method and application thereof, and belongs to the technical field of medicines, the compound can effectively degrade HDAC3, and further mechanism studies (including Western Blot and quantitative proteomics) prove that a representative compound can effectively degrade the HDAC3 by inducing the degradation of the HDAC3 through specificity. Further, an NLRP3 inflammasome pathway is inhibited, and finally, maturation and secretion of downstream inflammatory factors IL-1beta and caspase-1 are blocked, so that a remarkable anti-inflammatory effect is achieved. A mouse acute shock and colitis model further verifies the excellent anti-inflammatory activity of the compound in vivo. The invention not only provides a novel HDAC3 degradation agent with good activity, but also lays a solid material and theoretical foundation for deep development of anti-inflammatory drugs based on the 18 beta-glycyrrhetinic acid structure.
Owner:SHENYANG PHARMA UNIV
Use of a combination of plasma proteins in screening for pancreatic cancer
PendingCN122109536AMedical data miningComponent separationPancreas CancersMass Spectrometry-Mass Spectrometry
The application belongs to the technical field of proteomics and clinical disease screening, and relates to application of a combination of plasma proteins in screening of pancreatic cancer. The application is based on mass spectrometry quantitative proteomics technology, screens and determines a marker combination composed of four plasma proteins of GCLC, ALAD, HAGH and SORD, and comprehensively analyzes relative abundance of the marker combination through a binary Logistic regression model to distinguish pancreatic cancer patients from healthy people. Experimental results show that the protein combination has high sensitivity, specificity and area under curve (AUC) in a screening set and an independent verification set, and has good stability and repeatability. The method has the advantages of non-invasiveness, feasible operation and applicability to population screening by taking plasma as a detection sample, and can provide a new technical means for early screening of pancreatic cancer.
Owner:ACADEMY OF MILITARY MEDICAL SCIENCES +2