33 results about "Sperm protein" patented technology

The invention relates to immunocontraceptive synthetic peptide and immunocontraceptive chimeric peptide, wherein the amino acid sequence of the immunocontraceptive chimeric peptide is shown as SEQ ID:1 in a sequence table, the amino acid sequence of the immunocontraceptive synthetic peptide hESP103-107 is shown as SEQ ID:2 in the sequence table, and the amino acid sequence of the immunocontraceptive synthetic peptide hIzumo216-220 is shown as SEQ ID:3 in the sequence table. The invention also provides the application of the chimeric peptide, the synthetic peptide hESP103-107 or the synthetic peptide hIzumo216-220 as immunocontraceptive vaccines observed in contraception. The invention researches the chimeric peptide comprising sperm protein hESP, hIzumo and tNASP as well as the cross reacting antigens of UU (Ureaplasma Urealyticum) for the first time, and immunized mice can obtains stronger and reversible antifertility effect.

The invention provides a quantitative detection method of sperm surface Izumo1 protein. The method comprises the following steps: performing separation and pretreatment of a sperm, performing preliminary treatment of the sperm to expose the Izumo1 protein epitope of the sperm, marking the Izumo1 protein of the sperm by using fluorescein, and performing detection by using a flow cytometer. According to Tthe method provided by the invention, utilizes the flow cytometry is utilized to perform quantitative detection on the Izumo1 protein in the human sperm for the first time, directly realizes precise quantification of the Izumo1 protein is directly realized, is easy to realize standardization in operation is easy to realize, is quick and accurate effects are realized, and is high inthe precision is high. Compared with traditional methods for qualitative or semi-quantitative detection of Izumo1 protein, such as cell immunofluorescence and fluorescence in situ hybridization, the method provided by the invention has the advantages of convenient detection, high precision, high speed and the like; the method can be applied to quantitative detection of expression of sperm surface Izumo1 protein and is easy to popularize; and the detection result can be used for performing auxiliary evaluation on the fertilization ability of the sperm, so that the method is suitable for practical application.

The invention discloses a sperm protein marker SPACA4 closely related to the reproductive performance of boars and its application. The invention provides a new sperm protein marker SPACA4 which is closely related to the reproductive performance of boars, and its relative content is significantly positively correlated with the reproductive performance of boars. The fertilization ability and reproductive performance of pig semen can be used to accurately evaluate the reproductive performance of existing boars. In production, the protein marker can be used to more accurately select and breed high-reproductive boars. Elimination of boars with poor reproductive performance is conducive to improving the reproductive performance and economic benefits of pig farms. It is convenient to use in production, and it only needs to detect the content of the semen protein marker SPACA4, and will not cause boar sampling damage and stress.

The present invention provides compositions and methods useful for regulating fertilization and for use as a contraceptive based on the discovery herein of an oocyte specific protein, SAS1R (Sperm Acrosomal SLLP1 Receptor), which is a sperm protein receptor. Six SAS1R variants, including the full length SAS1R, were identified. mSLLP1 and SAS1R co-localized to oocytes and to acrosomes of acrosome-reacted sperm. Interactions between mSLLP1 and SAS1R were demonstrated by far-western analysis, in a yeast two-hybrid system under stringent selection conditions, and by immunoprecipitation of SAS1R by anti-mSLLP1 as well as the converse. Purified recombinant SAS1R was found to have protease activity, to inhibit fertilization in-vitro, and to induce an immune response in females. Together, the results suggest SAS1R is a proteolytically active, oocyte and early embryo specific oolemmal metalloprotease receptor for the sperm intra-acrosomal ligand SLLP1 and is a target for regulating fertilization and as a contraceptive.

Provided is a method for screening a biomarker related to severe oligoasthenospermia. The method comprises firstly carrying out mass spectrometry on non-coding amino acids of multiple groups of sperm proteins in the case of severe oligoasthenospermia by means of a NanoHPLC-MS / MS mass spectrometry system and an non-labelled quantitative proteomics method; then searching for the mass spectrometry data by means of a non-limiting amino acid protein modification analysis method, and carrying out multivariable Gaussian mixture distribution clustering analysis to identify the non-coding amino acids in the sperm protein groups to the greatest possible extent; and finally, comparing the non-coding amino acids of normal and patient sperm protein groups to obtain non-coding amino acid sites of the protein related to severe oligoasthenospermia, wherein same are thereby used as molecular markers of severe oligoasthenospermia. The method provides new diagnostic and therapeutic targets for severe oligoasthenospermia.

The invention discloses a sperm piRNA and a sperm protein MitoPLD as biomarkers for detecting and predicting male infertility. The biomarkers include 10 piRNAs (mainly piR-1207 and piR-2107) and the MitoPLD protein. The piRNA combination and the MitoPLD protein can be used for detection and prediction of sperm motility. According to the sperm piRNA and the sperm protein MitoPLD as the biomarkers for detecting and predicting the male infertility, spermatozoa are more easily obtained without other tissues, and the sperm piRNA and the sperm protein MitoPLD as the biomarkers for detecting and predicting the male infertility belong to non-invasive examination; the sperm piRNA and the MitoPLD protein can reflect pathological and physiological conditions throughout spermatogenesis at a molecularlevel, and are used as an auxiliary detection index to improve accuracy of detection, and provide potential targets for treatment of male reproductive dysfunction.