The invention discloses a Norovirus GI, GII and GIV nucleic acid genotyping reagent kit. The reagent kit comprises a viral nucleic acid quick extraction reagent, a PCR amplification reagent, PCR enzyme mixed liquor, a Norovirus GI, GII and GIV nucleic acid detection reagent, a positive control product, and a negative control product. The formula can be used for quick extraction of virus RNS, and the PCR detection efficiency is improved. The Norovirus GI, GII and GIV nucleic acid detection reagent comprises a Norovirus GI amplification primer F1, R1 and probe P1, a Norovirus GII amplification primer F2, R2 and probe P2 and a Norovirus GIV amplification primer F3, R3 and probe P3; and the sequence of the primer probe is created for the first time and unique in design, good in specificity and high in sensitivity. The invention further provides a Norovirus GI, GII and GIV nucleic acid genotyping detection method. According to the Norovirus GI, GII and GIV nucleic acid genotyping reagent kit and detection method disclosed by the invention, whether Norovirus GI, GII and GIV pathogens are contained in samples to be detected can be detected in a multichannel manner at the same time, quick and precise detection can be realized, viruses can be quantified, and application is convenient.