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Evaluation method of sperm quality

A sperm quality, sperm technology, applied in the field of biological detection

Active Publication Date: 2022-03-25
ZHEJIANG PROVINCIAL PEOPLES HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is currently no specific method report on the relationship between proteasome and semen quality parameters for sperm quality assessment

Method used

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  • Evaluation method of sperm quality
  • Evaluation method of sperm quality
  • Evaluation method of sperm quality

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Experimental program
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Embodiment

[0051] 1. Materials and methods

[0052] 1. Main test reagents

[0053]

[0054] 2. Preparation of main reagents

[0055] 1. Preparation of Proteasome Detection Reagents

[0056] ① Purchase Proteasome-Glo from Promega TM Chymotrypsin-Like Assay kit detects chymotrypsin activity.

[0057] ②The kit includes: 10ml proteasome buffer, 1 bottle of luciferin detection reagent, 50μl Suc-LLVY-Glo TM substrate.

[0058] ③Mix 10ml of proteasome buffer with 1 bottle of luciferin detection reagent to obtain 10ml of proteasome luciferin detection buffer. Use a 1.5ml brown EP tube to divide it into 20 portions of 500 μl each, and store it in a -20°C refrigerator.

[0059] ④ Add 50μl Suc-LLVY-Glo TM Substrate, according to 2.5μl portion, divided into 20 portions into PCR tubes, and stored in -20°C refrigerator.

[0060] ⑤ Mix proteasome fluorescein detection buffer with Suc-LLVY-Glo TM The substrate was equilibrated to room temperature, prepared according to the ratio of 200:1, m...

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Abstract

The invention discloses a sperm quality evaluation method, and belongs to the technical field of biological detection. According to the evaluation method, the proteasome activity in semen is taken as an index, the proteasome activity among different age groups, different sperm motility groups, different DNA fragment groups, different morphological rates and different head malformation rate groups is measured, the proteasome activity among different semen quality index groups is found to have no significant difference, but the higher the sperm proteasome activity expression is, the higher the sperm proteasome activity expression is, the higher the sperm proteasome activity expression is, the higher the sperm proteasome activity is. The fertilization rate and 2PN rate of conventional in-vitro fertilization are higher. Therefore, the sperm proteasome activity of a patient with unknown sterility or low conventional in-vitro fertilization rate can be considered and detected, and a basis is provided for clinically selecting conventional in-vitro fertilization or ICSI treatment.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a method for evaluating sperm quality. Background technique [0002] The 26S proteasome is the end of the ubiquitin-proteasome pathway. It is a complex multi-subunit complex composed of 19S regulatory particles (Regulatory particle, RP) responsible for recognizing and unfolding ubiquitinated substrates and protein degradation proteins. 20S catalytic core (Core peptidase, CP) composition. 19S RP can be biochemically divided into two parts: "base" and "lid". The base contains 6 kinds of ATPase subunits and 4 kinds of non-ATPase subunits, and the non-ATPase subunits are ubiquitin and ubiquitin-like proteins Provide the binding site, and the ATPase subunit has the activity of activating substrate protein unfolding. The lid part is composed only of non-ATPase subunits, recognizes polyubiquitin-bound protein substrates, and has deubiquitinating activity, which can unrave...

Claims

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Application Information

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IPC IPC(8): C12Q1/37C12Q1/24G01N21/64
CPCC12Q1/37C12Q1/24G01N21/6428G01N2333/96425
Inventor 陈小攀王方玉王成路舒崇医舒静高方郑珉杨雷香陈林洁
Owner ZHEJIANG PROVINCIAL PEOPLES HOSPITAL
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