281 results about "Sperm motility" patented technology

The invention provides a frozen semen diluent formula for flocks and herds, and the formula of each 100mL of diluent comprises the following components: 1.35-1.55g of citric acid, 2.4-2.6g of trihydroxymethyl aminomethane (tris), 1.0-1.2g of glucose, 4.5-6.8ml of glycerol, 18.0-21ml of yolk, 4.5-7.5ml of bovine serum albumin (BSA), 0.45-0.75ml of vitamin B12, 50000-100000IU of benzylpenicillin potassium, 50000-100000IU of streptomycin sulfate and the balance of distilled water. The formula provided by the invention is low in cost, simple and convenient in preparation and using and operation steps, capable of effectively improving the sperm motility, the acrosome integrity rate and the plasmalemma integrity rate, and capable of reducing the sperm aberration rate.

The invention discloses a microfluidic biological chip sperm quality analyser, which comprises a biological chip and a measurement host. The biological chip comprises a sample input module and a microchannel. The measurement host comprises a chip butt module, a fluid driving module, a signal detection processing module and a result display recording module. Driven by the fluid driving module, a sperm sample flows at a set flow velocity and in a set mode in the microchannel of the biological chip, and the sperm amount, the sperm concentration and the sperm motility in the tested sperm sample are obtained by the coulter principle and a unique algorithm. The invention also provides a sperm quality analysis method. The analyser of the invention realizes fully automatic high-throughout rapid analysis of the sperm sample. A user only needs to insert the biological chip comprising the sample into the host, then the analyser can rapidly output a test result report. The analyser has simple and convenient operation and low testing cost and is suitable for medical establishments at all levels and households.

Invented is a method of inhibiting the activity/function of PI3 kinases using pyridosulfonamide derivatives. Also invented is a method of treating one or more disease states selected from: autoimmune disorders, inflammatory diseases, cardiovascular diseases, neurodegenerative diseases, allergy, asthma, pancreatitis, multiorgan failure, kidney diseases, platelet aggregation, cancer, sperm motility, transplantation rejection, graft rejection and lung injuries by the administration of pyridosulfonamide derivatives.

The invention discloses a selenium-enriched trace element beverage and a preparation method thereof. The trace element beverage includes liquid tea beverage, coffee beverage, fruit juice beverage, soybean milk beverage, beer and drinking water added with selenium, and also includes solid tea products, Coffee products, fruit juice products, bean products, dairy products and other granulated beverages can maintain the original flavor and taste of the beverage, and at the same time have the unique anti-cancer and detoxification, anti-oxidation, anti-aging, prevention and treatment of Keshan disease, high blood pressure, Coronary heart disease, diabetes, enhance immunity, improve sperm motility, enhance liver activity, maintain heart function, remove heavy metal toxicity, accelerate detoxification and other health effects of trace element drinks. The beverage is not only suitable for daily selenium supplementation of normal people, but also suitable for intensive selenium supplementation of selenium-deficient people.

The invention discloses a method for artificial propagation of giant salamanders and cultivation of baby giant salamanders, which is characterized in that an ecological giant salamander farm is built in an original ecological region, the water in a cultivation pond comes from ravine streams of the original ecological region, the male giant salamanders are ecologically cultivated in classified ponds in different stages, the female giant salamanders are cultivated through indoor artificial cultivation, the male parent giant salamanders are specially cultivated by controlling water quality and water temperature and timely deploying nutrition, so that sperm motility of the male parent giant salamanders can be improved, and reproductive rate of the giant salamanders is further improved by performing selection, artificial hastened parturition and artificial fertilization on the parents; the propagated baby giant salamanders are cultivated in three stages, and disease prevention for the baby giant salamanders is performed in the feeding process. With the method of the invention, productivity of the artificially propagated giant salamanders can be more than 90%, the rate of artificial fertilization can be more than 80%, and hatchability can be more than 83.51%. By applying the method, population quantity of the giant salamanders can be improved, and scaled cultivation of the giant salamanders can be implemented.

The invention relates to Chinese herbal medicine feed additive for stud rams, which mainly solves the problem that the sperm quality of stud rams is reduced due to centralized and high-intensity sperm collection in the prior art. The Chinese herbal medicine feed additive is prepared by crashing and mixing epimedium, actinolite, Chinese dodder seed, raspberry, Chinese magnoliavine fruit, astragalus root and dangshen and can be added into feed for usage. The invention improves ram sexual appetite, prompts spermatogenesis, increases sperm motility, effectively improves reproductive performance, solves the problem that the sperm quality of stud rams is reduced due to centralized and high-intensity sperm collection in the prior art according to spermatogenesis principle.

The invention discloses an anti-freezing agent and an anti-freezing diluent for freezing and storing livestock sperm and a preparation method thereof. The anti-freezing agent for freezing and storing bovine and porcine sperm is algin, the amount of the algin added into the conventional diluent is 60 to 110 milligrams, wherein the conventional diluent is prepared from 1.1 grams of glucose, 1.48 grams of citric acid, 2.42 grams of Tris, 10 percent of fresh egg yolk, 0.06 gram of penicillin sodium and 0.1 gram of streptomycin sulfate, which are dissolved in 100 millimeters of distilled water. The adding amount of the algin for cattle is 100 to 110 milligrams, and the adding amount of the algin for pigs is 60 to 80 milligrams. The preparation method comprises the following steps of: adding 100 to 110 milligrams of algin and 4 percent glycerin (for cattle) or 60 to 80 milligrams of algin and 1 percent glycerin (for pigs) into the conventional diluent to prepare the anti-freezing diluent; and adjusting the pH value of the solution to be between 6.5 and 7.5, filtering and sterilizing the anti-freezing diluent under the osmotic pressure of 286mOsm/L, and putting the anti-freezing diluent into a 4 DEG C refrigerator for later use. The algin is used for freezing and storage of the bovine and porcine sperm for the first time, and after unfreezing, the sperm motility rate, the acrosome completeness rate and the plasma membrane completeness rate are 65 percent, 69 percent and 72 percent (for cattle) respectively and 58 percent, 62 percent and 63 percent (for pigs) respectively.

Invented is a method of inhibiting the activity/function of PI3 kinases using quinoxaline derivatives. Also invented is a method of treating one or more disease states selected from: autoimmune disorders, inflammatory diseases, cardiovascular diseases, neurodegenerative diseases, allergy, asthma, pancreatitis, multiorgan failure, kidney diseases, platelet aggregation, cancer, sperm motility, transplantation rejection, graft rejection and lung injuries by the administration of quinoxaline derivatives.

Invented is a method of inhibiting the activity/function of PI3 kinases using naphthyridine derivatives. Also invented is a method of treating one or more disease states selected from: autoimmune disorders, inflammatory diseases, cardiovascular diseases, neurodegenerative diseases, allergy, asthma, pancreatitis, multiorgan failure, kidney diseases, platelet aggregation, cancer, sperm motility, transplantation rejection, graft rejection and lung injuries by the administration of naphthyridine derivatives.

A method and kit for enhancing the natural fertility process. The kit includes a vaginal douche that is used prior to intercourse to enhance the sperm transportation and sustaining properties of the cervical mucous. The douche contains a balanced electrolyte solution, polysaccharides, and pH buffers. The kit also includes nutriceuticals specifically formulated for both the male and female which include amino acids, minerals, vitamins, herbs, phytoestrogens, and antioxidants along with a specified dosing regimen. A basal body temperature thermometer and chart is provided with instructions to confirm when and if ovulation will/did occur. Commercially available urinary chemical reagent strips are provided with instructions so as to predict/confirm if and when ovulation will occur. A lubricating medium will also be provided and utilized at the time of intercourse which is nonspermicidal and which contains polysaccharides which influence natural sperm motility. A detailed instruction book regarding the method and practice is provided along with dietary and lifestyle recommendations which have been shown to affect natural fertility.

The invention discloses semen diluent and a method for artificial insemination of breeding hens at an interval of six days. The semen diluent comprises semen diluent 1, semen diluent 2 and semen diluent 3; the semen diluent is prepared from the following ingredients in part by weight: 5-7 parts of monosaccharide, 7.2-10.1 parts of buffering substance, 1.5-2.8 parts of antibiotics, 0.2-1.2 parts of weak electrolyte, 6.4-8.3 parts of vitamin, 0.3-0.5 parts of EDTA-Na (ethylenediamine tetraacetic acid disodium salt) or 1.5-2 parts of lycopene. The semen diluent is adopted for artificial insemination by adopting a six-day interval method, the sperm motility is effectively improved, the fertilization probability is increased, the labor intensity of workers is effectively reduced, the investment of labor cost is also reduced, and working stress caused by less breeders can be alleviated.

A vaginal lubricant comprising high molecular weight hyaluronic acid combined with low molecular weight hyaluronic acid and/or chitosan which has improved stability and which has applications in, for example, maintaining and/or promoting sperm motility.

A method and device for assaying sperm motility in a forward direction and density of active sperm in a semen sample are disclosed. The device includes a microfluidics structure having a sample reservoir, a downstream collection region and a microchannel extending therebetween. The microchannel is dimensioned to confine sample sperm to single-direction movement within the channel, such that sperm in a semen sample placed in the sample reservoir enter and migrate along the microchannel toward and into the collection region. Also included is a detector for detecting the presence of labeled sperm in the microchannel or collection region, and an electronics unit operatively connected to the detector for (i) receiving detector signals, (ii) based on the detector signals received, determining sperm motility and density in the sperm sample, and (iii) displaying information related to sperm motility and density.

Invented is a method of inhibiting the activity/function of PI3 kinases using quinazoline derivatives. Also invented is a method of treating one or more disease states selected from: autoimmune disorders, inflammatory diseases, cardiovascular diseases, neurodegenerative diseases, allergy, asthma, pancreatitis, multiorgan failure, kidney diseases, platelet aggregation, cancer, sperm motility, transplantation rejection, graft rejection and lung injuries by the administration of quinazoline derivatives.

The invention relates to a botanical drug compound recipe for preventing and treating microwave radiation injury. According to the invention, it is found that botanical drugs mainly consisting of peony root, milkvetch root, duckweed and red sage root can be used for preparing a medicine used for preventing and treating microwave radiation injury. According to results of abundant experiments of detection of learning and memory capacity of rats, hippocampal amino acid neurotransmitters, sperm motility, the rate of teratosperm, myocardial metabolic enzyme (CK-MB, LDH and AST activity) and the content of Ca<2+> in serum of rats, the number of peripheral blood lymphocytes and the like, the medicine provided in the invention has an obvious treatment effect on injury to the brain, reproduction, the heart and immunity of rats caused by microwave radiation, and the effect is superior to that of medicines on the market.

The invention discloses poultry semen diluent for maintaining the sperm motility and prolonging the insemination time. Every 1000ml of diluent comprises the following components: 0.5g to 1.4g of magnesium chloride, 1g to 1.3g of potassium citrate, 8g to 10g of sodium acetate, 10g to 20g of fructose, 10g to 25g of sodium glutamate, 1g to 5g of multi-vitamin and water. The poultry semen diluent has the beneficial effects that the insemination time can be prolonged, the number of the raised breeder cocks can be reduced, the stress on a breeder hen caused by the artificial insemination can be alleviated, the egg laying rate can be improved, the fertility rate can be increased, the incidence probability of the oviduct inflammatory can be reduced, and the quality of the chicks can be improved.

The invention discloses an in-vitro culture system kit for improving the sperm motility of human bodies and an application method thereof. The kit comprises 9 mL of mHTF, 1 mL of serum substitute vcl recombinant proteins, and 45-55 mu g of acat2 recombinant proteins. The application method is implemented through mixing and balancing reagents in the kit for 2-3 hours at a temperature of 37 DEG C, so that 10 ml of a sperm culturing system is obtained; and after sperms separated from a sperm division fluid is resuspended by using the sperm culturing system according to a concentration of 10 millions/mL, gently and uniformly mixing, putting the obtained product in a thermostat at a temperature of 37 DEG C for 90-120 minutes. The forward motion activity of sperms resuspended by using the sperm culturing system in the invention can be significantly improved, and after the sperms are used in artificial insemination and in-vitro fertilization technologies, the pregnancy outcome can be improved, and the fertilization rate can be increased; and a new method is also provided for the treatment of patients with serious asthenospermia.

The invention discloses a composition for improving human sperm motility and quality, which comprises zinc, selenium, vitamin E, folic acid, vitamin B12, vitamin C, fructose, and citric acid. The composition of the invention is safe and effective for long-term administration, and has significant safety.

The invention provides a fluorescent staining method of evaluating sperm motility of a tree shrew. The fluorescent staining method comprises the following steps: (1) preparing a TALP-HEPES sperm rinse; (2) collecting sperms; (3) washing the sperms; (4) staining; and (5) detecting by a microscope. According to the fluorescent staining method provided by the invention, by utilization of the characteristics that Hoeches 333422 and PI are different in reaction to dead and alive sperms and can be excited by a laser with a same wavelength, fluorescent staining is carried out on sperms of the tree shrew, and the sperm motility can be accurately detected without needing to switch a light source; in addition, as the two dyes respectively emit red and blue fluorescence after being excited by the laser, a dye which emits green fluorescence can also be introduced to detect the integrity of sperm acrosomes while the motility is detected, and the sperm motility and the integrity of the sperm acrosomes of the tree shrew are accurately detected by virtue of a fluorescence microscope or a flow cytometer.

The invention discloses a fluorescent staining method for evaluating sperm motility of domesticated mammals. The fluorescent staining method comprises the following step: according to the characteristic that a mammal live sperm probe Hoechst 333422 and a mammal dead sperm probe PI can be excited by the same ultraviolet light to emit fluorescent light, performing fluorescent staining on sperms of four important domesticated mammals which are pigs, cows, sheep and dogs to accurately perform sperm mobility evaluation. Compared with the conventional PI/SYBR-14 staining method, the PI/Hoechst 33342 staining method adopts Hoechst 33342 to substitute an expensive SYBR-14 probe, so that the detecting cost is greatly lowered. The fluorescent staining method can be used for successfully evaluating the sperm mobility of important domesticated mammals such as pigs, cows, sheep and dogs, and can be widely applied to evaluating the sperm motility of various domesticated mammals.

The invention relates to sperm motility fluid and a preparation method thereof, belonging to the technical field of human assisted reproduction. The sperm motility fluid is prepared by dissolving ATPsodium salt, dried heparin sodium powder/heparin sodium salt solution into a salt solution containing antibiotics and indicators. The ATP sodium salt and the heparin sodium are added into the sperm motility fluid disclosed by the invention, the ATP sodium salt can provide energy substances for sperm motility and contribute to sperm middle-piece mitochondria capacity, the heparin can activate a receptor-coupled signal transduction system by binding with a receptor on the sperm surface, the capacity function and calcium influx of the sperm mitochondria are gently and effectively improved, and the sperm motility is further improved. Therefore, the sperm motility can be effectively improved, the capacity of the sperm middle-piece mitochondria is wakened and activated, and the success rate of intra-uterine insemination (IUI) is improved. Moreover, a powerful guarantee is provided for the safety of an assisted reproductive technology.

A method and device for assaying sperm motility in a forward direction and density of active sperm in a semen sample are disclosed. The device includes a microfluidics structure having a sample reservoir, a downstream collection region and a microchannel extending therebetween. The microchannel is dimensioned to confine sample sperm to single-direction movement within the channel, such that sperm in a semen sample placed in the sample reservoir enter and migrate along the microchannel toward and into the collection region. Also included is a detector for detecting the presence of labeled sperm in the microchannel or collection region, and an electronics unit operatively connected to the detector for (i) receiving detector signals, (ii) based on the detector signals received, determining sperm motility and density in the sperm sample, and (iii) displaying information related to sperm motility and density.