142 results about "Sperm motility" patented technology

The invention discloses a bull sperm motility related SNP molecular marker based on an HSPB9 gene, a detection product and application, and belongs to the technical field of molecular biology, the SNP molecular marker is located at the position of a bovine genome Chr19: 42255643, and the polymorphism is C or T. The research finds that the SNP genotype combination with high sperm motility of the bull is TT. By detecting the SNP of the SNP site, the sperm motility evaluation of the bull can be realized, the breeding is assisted, a reliable support is provided for breeding a high-quality next generation, and the method has the advantages of high reliability, high efficiency, low cost and the like.

The invention belongs to the technical field of biological medicines, and particularly relates to a sperm injury repair model and an establishment method thereof. Comprising the following steps: step 1, establishing a sperm lead poisoning model; and 2, establishing a lead poisoning sperm repair model. The method for establishing the lead poisoning sperm repair model in the step 2 comprises the following steps: (1) preparing a preparation for optimizing sperm functions in an extracellular vesicle way; (2) repairing the damaged sperms by using the preparation for optimizing the sperm function through the extracellular vesicles; and (3) detecting the activity of the repaired sperms. In the invention, by establishing a sperm injury repair model and adopting an extracellular vesicle modification technology, the key functional protein is introduced into the extracellular vesicle, and the method has important significance on auxiliary researches such as development and research of related drugs for improving sperm injury and improving sperm motility of asthenospermia patients in vitro.

Means for rigorous quantitative assessment of sperm motility are presented, based on a number of movement and morphology parameters measured using various image processing methods. The quantitative assessment thereby achieved is objective and multidimensional, allowing for detailed and repeatable assessment of samples. Deep learning methods are also disclosed for detection and classification of sperm cells (or other types of cells or particles) using neural networks, adapted to work both in good and in poor imaging condition, such as low magnification and resolution. Specific methods for both supervised and unsupervised deep learning approaches are delineated. In a non-limiting disclosure, the methods are particularly adapted to deal with cases of azoospermia, where there is a very low number of sperm cells (most of which do not swim) and a lot of debris in the imaged field of view.

The invention provides a sperm automatic detection and tracking method, sperm automatic detection and tracking equipment and a storage medium. The method comprises the following steps: firstly, preprocessing a sperm image, performing rotation bounding box labeling on a head and a tail, and generating a training set containing a center point coordinate, a width, a height and an angle; training a target detection model by using the training set to obtain a model capable of automatically identifying sperms and outputting a rotating bounding box; detecting video frames by using the model, and extracting the position, orientation and size of each frame of sperm; a multi-dimensional fusion scoring system is constructed through position, direction, speed, color and track continuity, adjacent frame targets are matched, a comprehensive score is generated, the targets are associated, and a continuous track and a unique identifier are formed and used for calculating motion parameters; and judging whether the track is static or low active according to the speed and displacement of the latest N frames, sealing the track to eliminate subsequent matching, and only reserving a non-sealed track to calculate motion parameters. According to the method, interference is effectively avoided, and the tracking accuracy and the reliability of sperm motility parameter calculation are remarkably improved.

The invention discloses an auxiliary reproductive semen treatment device matched with different insemination modes, which comprises a box body with an openable box door, a heating treatment table is arranged at the center of the bottom in the box body, and a treatment plate is placed on the heating treatment table; a control box is arranged at the top, a control circuit board is arranged in the control box, a heat dissipation plate and a display screen are arranged, and keys and knobs are arranged on the two sides; the back of the box is provided with a power interface. Through modular design, the device is compatible with semen treatment of various insemination modes such as IVF, ICSI and IUI; the first processing plate (transverse groove method) and the second processing plate (screen method) are used for screening sperms, through natural movement ability, form and thermotaxis guidance, mechanical damage is reduced, sperm motility is improved, sperm DNA fragment rate is reduced, and therefore ovum fertilization rate, embryo quality and pregnancy outcome are expected to be improved.

The invention discloses a feed for improving the quality of semen of an old boar and an application of the feed. The feed disclosed by the invention is prepared from the following components in parts by weight: 8 to 10 parts of corn, 18 to 23 parts of red sorghum, 37 to 43 parts of hulled barley, 12 to 17 parts of bran, 0.5 to 1.5 parts of functional fiber, 7 to 9 parts of soybean meal, 1.5 to 2.5 parts of fish oil or microalgae powder, 1.3 to 1.4 parts of mountain flour, 1 to 1.2 parts of monocalcium phosphate, 0.4 to 0.5 part of sodium chloride, 0.2 to 0.3 part of lysine, 0.1 to 0.15 part of L-threonine, 0.1 to 0.15 part of choline chloride, 1 to 1.5 parts of betaine and 0.5 to 1 part of a premix for boars. After the feed is applied, the total effective sperm number of the aged boars is remarkably increased, the sperm motility and form are improved, the breeding age limit is prolonged, the production value is increased, and the biological safety risk caused by the high renewal rate is reduced.

The invention discloses a penaeus monodon sperm ultralow-temperature preservation regulation and control method based on sperm motility detection, and relates to the technical field of aquatic product germplasm resources and genetic breeding, and the method comprises the following steps: collecting a penaeus monodon sperm sample, preprocessing the sample to carry out sperm motility detection, and utilizing deep learning to assist sperm motility analysis to obtain sperm motility data; collecting multiple omics data of the preprocessed sperm sample, and screening key molecular markers significantly related to sperm motility by using a knowledge graph to obtain omics characteristics; constructing a freezing injury prediction model according to the improved graph neural network, and inputting sperm motility data and omics characteristics to predict the freezing injury risk grade of the sperm sample; and dynamically adjusting a cryopreservation scheme based on the cryogenic injury risk grade of the sperm sample. According to the method, through a whole chain strategy of sperm motility detection, multi-omics analysis, artificial intelligence prediction and dynamic regulation and control, precise optimization of cryopreservation of the sperms of the penaeus monodon is realized.

The invention relates to the technical field of livestock and poultry breeding, in particular to a goat hybridization breeding method capable of achieving complete-cycle nutrition enhancement. The core of the method is that systematic nutrition intervention runs through the whole breeding process. Before hybridization, performing differential nutrition pretreatment on the parents for 60 days: feeding the female parents with a breeding nutrition enhancer added with components such as D-chiro-inositol and N-acetylcysteine, and supplementing sperm motility enhancer containing acetylated L-carnitine and ergothioneine to the male parents. In the gamete treatment stage, the oocytes are subjected to in-vitro maturation culture in a special culture solution containing components such as D-chiro-inositol and a growth differentiation factor 9; after the sperms are subjected to gradient centrifugal screening, completing in-vitro fertilization in an optimized fertilization culture solution; after the embryos develop into blastocysts in the sequential culture system, carrying out laparoscopic transplantation, and carrying out staged precise nutrition culture on the pregnant ewes. According to the method, through multi-level nutrition enhancement, the reproductive capacity of the goats and the growth performance of offspring are effectively improved.

The application provides a sperm automatic detection and tracking method, device and storage medium. The method first pre-processes a sperm image, performs head-tail rotation boundary box labeling, and generates a training set containing center point coordinates, width, height and angle; a target detection model is trained using the training set to obtain a model capable of automatically identifying sperm and outputting a rotation boundary box; the model is applied to detect video frames to extract the position, orientation and size of each frame of sperm; a multi-dimensional fusion scoring system is constructed through position, direction, speed, color and trajectory continuity to match adjacent frames of targets, generate a comprehensive score and associate the targets to form a continuous trajectory and a unique identifier for calculating motion parameters; and then whether the trajectory is stationary or low active is judged according to the speed and displacement of the last N frames, the trajectory is sealed to exclude subsequent matching, and only the non-sealed trajectory is retained to calculate the motion parameters. The method effectively avoids interference, significantly improves the tracking accuracy and the reliability of sperm motility parameter calculation.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker related to sperm motility on a pig chromosome 7, a primer pair and application of the SNP molecular marker and the primer pair. SNP loci correspond to AVt of 22th, 074th and 230th loci on the chromosome 7 of a reference sequence of an international pig genome version 11.1; g mutation; the SNP molecular marker related to sperm motility on the pig chromosome 7 can explore the genetic mechanism of complex characters by utilizing regulation and control annotation, so that the probability of finding reliable association is enhanced, the test efficiency can be improved, and the accuracy and reliability of the result are improved.

The invention discloses a method for predicting the sperm motility of a breeding bull based on a host SNP-rumen microorganism-metabolite interaction axis, and the sperm motility of the breeding bull can be obtained by detecting the haplotype condition of SNP1-SNP3 and / or SNP4-SNP6 sites of a breeding bull genome. The method breaks through the limitations of strong dependency on phenotype endpoint evaluation and prediction lag in the current bull seed selection process, and realizes forward accurate prediction and early evaluation of the semen quality of the breeding bull.

The invention relates to the technical field of semen preservative preparation, in particular to a diluent preparation capable of prolonging the normal-temperature survival time of boar semen and a preparation method of the diluent preparation. Glucose, L-glutamine, an antioxidant and polysaccharide components are scientifically proportioned, and an innovative polyethyleneimine-polysaccharide copolymer is combined, so that the normal-temperature survival time of boar semen is prolonged; the dual effects of sperm metabolism regulation and cell membrane protection are realized. The polyethyleneimine-polysaccharide copolymer enhances the biocompatibility and protective ability of the diluent through stable cross-linking of amino and carboxyl, and effectively relieves the problem of sudden change of osmotic pressure and acidity of the traditional preservative. In the formula, antioxidant components, such as reduced glutathione, vitamin E and coenzyme Q10, synergistically reduce oxidative stress and delay sperm senescence. According to the whole formula, proper pH and osmotic pressure are guaranteed, the sperm motility and survival time are remarkably prolonged, the requirement of pig industry for long-term preservation of sperm is met, and the feed additive has remarkable application value and popularization potential.

The invention relates to the technical field of cock breeding, in particular to a biomarker, a preparation method and application thereof and a method for assisting in breeding cock semen quality identification. The biomarker comprises the following components: phosphatidylcholine, lysophosphatidylcholine, phosphatidyl ethanolamine, lysophosphatidyl ethanolamine, phosphatidylserine and lysophosphatidylserine. According to the invention, key markers can be rapidly detected from the semen so as to determine breeding suitable for liquid-state preservation of the semen and high-activity individuals. The artificial insemination interval time can be greatly prolonged, and long-time high-sperm active breeding cock individuals can be rapidly bred. The breeder cock breeding amount can be reduced, grains are saved, and breeding benefits are greatly improved.

The invention discloses a yak frozen semen freezing process intelligent control method based on dynamic cooling curve optimization, and relates to the field of animal frozen semen preservation, and the yak frozen semen freezing process intelligent control method comprises the following steps: arranging a plurality of frozen semen tubes filled with consumed cattle semen on an annular tray with a cold source in the center in a radioactive manner, and uniformly arranging the plurality of frozen semen tubes in a surrounding manner in each radial distance interval; in the freezing process, cold energy is output according to a preset cooling program, and the annular tray is driven to rotate; unfreezing operation is executed, sperm motility values are measured, interval motility values of the sperm motility values of the frozen sperm tubules in all the radial distance intervals are counted, a temperature curve at the radial distance interval with the highest interval motility value is determined, a cooling program is modified in multiple rounds of experiments, and the process is repeated, so that the temperature curve is determined. And clustering the obtained temperature curves and selecting an optimal target temperature curve to perform freezing control. According to the method, the problem that the optimal dynamic cooling curve is difficult to efficiently obtain in the freezing process of the frozen yak sperms is solved.

The present invention discloses a protective agent for endogenous iron death inhibitory protein in sheep sperm, a protective method and an application thereof. The protective agent for endogenous iron death inhibitory protein in sheep sperm comprises the following components: Tris, citric acid, glucose, chloroquine, glycerol, a mixture of penicillin-streptomycin, egg yolk liquid and ultrapure water, and the above substances are dissolved in the ultrapure water. The present invention also provides a method for protecting endogenous iron death inhibitory protein in sheep sperm, comprising the following steps: preparing an endogenous iron death inhibitory protein protective agent for sheep sperm; semen collection; diluting semen with an endogenous iron death inhibitory protein protective agent for sheep sperm; and freezing semen. The present invention also provides an application of an endogenous iron death inhibitory protein protective agent for sheep sperm. The present invention can protect the iron death inhibitory protein during the freezing recovery process, ensure the expression level of the iron death inhibitory protein, ensure the sperm motility and the integrity of the biofilm structure, and greatly improve the resistance of sheep sperm to freezing damage.

The invention belongs to the technical field of medicines, and discloses a gastrodia elata microbial fermentation product as well as a preparation method and application thereof. According to the invention, three aspergillus oryzae are utilized to successfully carry out microbial fermentation on gastrodia elata, the gastrodia elata microbial fermentation product of which the content of active ingredients gastrodin and p-hydroxybenzyl alcohol is obviously increased is obtained, and the pharmacological action of the gastrodia elata microbial fermentation product on treating or improving oligoasthenospermia is verified through an oligoasthenospermia mouse model; results show that the gastrodia elata microbial fermentation product has better effects of improving sperm concentration, enhancing sperm motility and rapidly moving forwards, can be used as an active ingredient of a medicine for treating or improving oligospermia and asthenospermia, and has wide application.

The invention discloses an application of a carrageenan degradation product in preparation of a medicine for improving male reproductive power. According to the method disclosed by the invention, a compound capable of improving sperm motility and male reproductive power is obtained, and a new thought and clue are provided for deeply researching the pathogenesis of oligoasthenospermia and researchers of male reproductive health in the future.

The invention relates to the technical field of image processing and reproductive hospital inspection, and provides a sperm movement tracking and quality analysis method based on deep learning and optical flow fusion, which comprises the following steps of: identifying sperms through a target detection model, acquiring a detection frame, and screening to obtain detection points; predicting the predicted position of the sperm in the current frame by using a sparse optical flow method based on the historical trajectory, performing data association on the predicted position and a detection point, establishing a matching relationship, updating the trajectory according to the matching relationship, establishing a new trajectory or terminating a lost trajectory, calculating kinematics parameters and sperm concentration based on the complete motion trajectory, and completing activity grading. According to the invention, through fusion of deep learning detection and optical flow prediction, high-precision identification under a complex background and stable tracking under a high-density cross scene are realized, the identity exchange rate is significantly reduced, and the shielding robustness is enhanced; and meanwhile, full-process automation from video input to clinical report is realized, and the accuracy, repeatability and clinical credibility of an analysis result are improved.

The utility model relates to the technical field of unfreezing devices, and discloses a bee frozen semen unfreezing device which comprises a shell, a heating assembly is arranged in the shell and used for unfreezing frozen semen, a mounting plate is fixedly connected to the interior of the shell, and the mounting plate is fixedly connected to the interior of the shell. A plurality of uniformly distributed electric push rods are fixedly connected into the mounting plate, the telescopic ends of the electric push rods are fixedly connected with moving rods, guide plates are fixedly connected to the left side and the right side of each moving rod, clamping plates abut against the front sides of the guide plates, and fixing shafts are rotatably connected to the middles of the clamping plates; and the fixed shaft is fixedly connected to the interior of the mounting plate. According to the device, the part, containing semen, of the semen straw vibrates in water so that the bee semen in the semen straw can be evenly heated, the recovery effect of sperms can be easily enhanced, the heat conduction plate and the heat dissipation plates can evenly transfer heat into water flow, the water flow is evenly heated, and it is prevented that the sperm motility is affected due to the fact that the temperature of the water flow is not uniform.

This invention discloses a pharmaceutical composition for treating asthenospermia by activating the Nrf2 / HO-1 / GPx4 pathway and its application. The pharmaceutical composition comprises taurine and geranium as active ingredients, wherein the mass ratio of taurine to geranium is 3-5:1. The pharmaceutical composition of this invention can synergistically activate the Nrf2 / HO-1 / GPx4 antioxidant signaling pathway, upregulate the expression of downstream antioxidant enzymes GPx4 and HO-1, inhibit lipid peroxidation, protect sperm mitochondrial function, and simultaneously improve the microenvironmental metabolic profile of the epididymis—the site of sperm maturation—thereby enhancing sperm motility. The pharmaceutical composition provided by this invention can be used to prepare a treatment for asthenospermia, particularly oxidative stress-induced asthenospermia.

The invention is applicable to the technical field of animal genetic engineering, and provides a DHX36 gene molecular marker related to bovine sperm motility traits and application thereof, the DHX36 gene molecular marker is a 231st locus of a bovine DHX36 gene segment as shown in a sequence table SEQ ID NO: 1, and the locus has a C-G base mutation. Based on the DHX36 gene molecular marker, the genotype is judged through sequence determination, and the genotype can be associated with the normal and unfrozen activity traits of bovine sperms. An analysis result shows that the bovine sperms of individuals with different genotypes are normal and have significant differences in vitality after unfreezing. According to the invention, the specific primer group is utilized to obtain the bovine DHX36 gene segment related to the normal and unfrozen activity traits of bovine sperms, and the specific SNP site in the segment is taken as the molecular marker, so that a theoretical basis and specific application are provided for marker-assisted selection of cattle.

The invention discloses a preparation method and application of a corn germ meal extract, the preparation method is used for extracting functional active ingredients capable of improving heat stress resistance of rabbits from corn germ meal through a specific enzymolysis process, and the preparation method has the advantages of efficient extraction, condition optimization, cost effectiveness and the like; in the aspect of application, the extract can remarkably reduce the rectum temperature of rabbits, relieve the adverse effect of heat stress on rabbit bodies and enhance the adaptive capacity of rabbits in a high-temperature environment; meanwhile, the feed intake of the female rabbits in the gestation period and the lactation period is improved, so that the farrowing rate of the female rabbits is improved, and the number and quality of young rabbits are increased; in addition, the extract can also provide necessary nutritional support for growth and development of the young rabbits, promote rapid growth of the young rabbits, shorten the breeding cycle and improve the breeding benefits; moreover, the sperm quality, including sperm motility, ejaculation amount, malformation rate and other indexes, of the breeding male rabbits is remarkably improved, so that the fertilization success rate is improved, and the number and quality of young rabbits are increased.

The invention relates to a chilo suppressalis seedless sperm formation factor TNNI3 and application thereof in male sterility. The invention finds that the TNNI3 gene specifically regulates and controls the chilo suppressalis seedless spermatogenesis process for the first time, and an RNA interference technology proves that TNNI3 can inhibit the number of chilo suppressalis seedless sperm bundles and has no obvious influence on the number of nucleated sperm bundles and sperm motility, so that the egg laying amount after mating of male insects and wild female insects can be obviously reduced, and the survival rate of the male insects and the wild female insects is improved. And a theoretical basis is provided for developing a novel prevention and treatment strategy for male reproduction.

The invention relates to a protective liquid formula for improving the survival rate of frozen and thawed sperms of pigs. The protective liquid formula comprises the following components in percentage by mass: 14% of sodium hexadecylsulfonate, 7% of coconut oil monoethanolamide and 79% of distilled water. The preparation method comprises the following steps: 1) weighing 14g of sodium hexadecylsulfonate and 7g of coconut oil monoethanolamide, and dissolving in 79g of distilled water; and 2) uniformly stirring the solution obtained in the step 1) at 60 DEG C until the sodium hexadecyl sulfonate and the coconut oil monoethanolamide are completely dissolved in the distilled water. The protection liquid has the advantages that the protection liquid protects porcine sperm cells, provides a suitable living environment for porcine sperms, improves the oxidative stress resistance and the preservation effect of the porcine sperms, ensures the survival rate of the porcine sperms, and further improves the breeding rate and the breeding benefits of sows; the survival rate, the sperm motility and the acrosome integrity rate of the frozen and thawed sperms of the pigs are obviously improved; the feed additive is simple in proportioning, high in reliability, low in economic cost, high in biological value and convenient to popularize and apply in production, and yolk with extremely high preparation requirements is not used.

The invention relates to the technical field of lion-head goose breeding, in particular to a method for screening and identifying genes related to lion-head goose sperm motility regulation and control, which comprises the following steps: dividing healthy lion-head male geese in a breeding period into a high sperm motility group and a low sperm motility group according to sperm motility, collecting two groups of testicular tissues and sperm samples, and preserving the testicular tissues at low temperature for molecular detection; performing transcriptome sequencing on the testicular tissue to obtain mRNA and non-coding RNA expression data; screening two groups of nucleic acid molecules with significant differential expression, and determining candidate genes through functional enrichment analysis of reproductive development related biological pathways; and verifying the authenticity of the expression difference of the candidate gene through fluorescent quantitative PCR, and finally determining the target gene. According to the method, related data are obtained through sequencing, the candidate genes are determined by screening differential molecules through enrichment analysis and PCR verification, accurate identification of a related gene system is achieved, and the problems of missing detection and insufficient accuracy caused by lack of transcriptome sequencing in a traditional method are solved.

The present invention relates to the technical field of antioxidant technology for livestock sperm, and provides a highly efficient antioxidant donkey sperm diluent, a preparation method thereof, and an application. A highly efficient antioxidant donkey sperm diluent includes a basal medium and additives, wherein: the basal medium contains the following components by weight in every 1000 mL of water: 30 - 60 g of glucose, 4 - 6 g of 4 - (2 - hydroxyethyl)piperazine - 1 - ethanesulfonic acid, 12 - 24 g of skim milk powder, and 0.5 - 1.1 g of potassium citrate; the additives contain the following components by weight in every 1000 mL of the basal medium: 5 mg - 10 mg of sulforaphane, 0.1 mg - 0.2 mg of stigmasterol, 40 μg - 100 μg of enrofloxacin concentrate, and 3 mg - 5 mg of cefotaxime sodium. The present invention can effectively improve the conception rate of female donkeys, reduce the incidence of metritis, and improve the in vitro sperm motility.

The present invention provides methods for diagnosing or predicting a fertility defect, such as infertility, in a mammalian subject by detecting TTC6. The invention also provides kits and instruments for diagnosing or predicting fertility defects, such as infertility disorders, in mammalian individuals by detecting TTC6. The invention finds and proves for the first time that the knockout of the Ttc6 gene leads to sperm motility weakening, induces sperm to rotate, finally destroys the fertilization function and leads to male infertility, thereby providing a method for diagnosing infertility disorder of mammals including humans, and promoting development of a new treatment method for male infertility individuals influenced by gene mutation.

The invention discloses a sperm composite lubricant and a preparation method thereof, and the method comprises the following steps: mixing a polymer natural humectant, degradable carrier gel and pyrroloquinoline quinone precursor liquid according to a preset proportion to obtain a pyrroloquinoline quinone-pre-embedded moisturizing gel matrix; the preparation method comprises the following steps: dissolving a lipid membrane stabilizer in an ethanol-water mixed solution for ultrasonic emulsification, dropwise adding the emulsified lipid membrane stabilizer into a pyrroloquinoline quinone-pre-embedded moisturizing gel matrix, and stirring to obtain lipid fusion type gel; suspending a sperm motility promoter microcapsule and a plant extract in a buffer solution to form a mixed suspension, adding the mixed suspension into the lipid fusion type gel to obtain active chimeric gel, dropwise adding an antioxidant into the active chimeric gel, and stirring to obtain a microcapsule-gel dual controlled release lubricant precursor; and filtering and sterilizing the microcapsule-gel double controlled release lubricant precursor to obtain the sperm composite lubricant. The sperm motility and survival rate can be improved.

The application discloses a Holstein cow sperm activity miRNA molecular marker selection method, which comprises the following steps: (1) specific primers are designed according to a Holstein cow miRNA precursor region, and PCR amplification is carried out on to-be-detected cow genomic DNA to obtain an amplification product; (2) the PCR product is subjected to enzyme cutting by using a BspE I restriction endonuclease, and electrophoresis typing is carried out, and three genotypes, namely, a TT type, a TC type and a CC type, are obtained; and (3) the sperm activity of the CC type is significantly higher than that of the TT type, and the sperm deformity rate of the CC type is significantly lower than that of the TT type, so the CC type and the TC type are selected for breeding, and the C genotype frequency is improved. The method can improve the accuracy of sperm quality selection of the cow, is used for the selection and breeding of high-quality cows, and provides a selection and breeding tool for strengthening the excellent germplasm of Chinese Holstein cows.

The invention provides a feed for improving sperm quality after sperm freezing as well as a preparation method and application thereof, and belongs to the technical field of animal feeds. The feed for improving the sperm quality of breeding male rabbits and improving the conception rate of female rabbits comprises the following components in parts by weight: 10-20 parts of corn, 50-60 parts of soybean meal, 12-20 parts of fish meal, 5-10 parts of premix and 0.1-0.3 part of vitamin E. The feed improves the sperm motility of fresh sperms, the sperm motility after freezing, the acrosome integrity rate, the plasma membrane integrity rate and the mitochondrial membrane potential of the sperms after the fresh sperms and the frozen sperms.