71 results about "Sperm motility" patented technology

Means for rigorous quantitative assessment of sperm motility are presented, based on a number of movement and morphology parameters measured using various image processing methods. The quantitative assessment thereby achieved is objective and multidimensional, allowing for detailed and repeatable assessment of samples. Deep learning methods are also disclosed for detection and classification of sperm cells (or other types of cells or particles) using neural networks, adapted to work both in good and in poor imaging condition, such as low magnification and resolution. Specific methods for both supervised and unsupervised deep learning approaches are delineated. In a non-limiting disclosure, the methods are particularly adapted to deal with cases of azoospermia, where there is a very low number of sperm cells (most of which do not swim) and a lot of debris in the imaged field of view.

The invention provides a sperm automatic detection and tracking method, sperm automatic detection and tracking equipment and a storage medium. The method comprises the following steps: firstly, preprocessing a sperm image, performing rotation bounding box labeling on a head and a tail, and generating a training set containing a center point coordinate, a width, a height and an angle; training a target detection model by using the training set to obtain a model capable of automatically identifying sperms and outputting a rotating bounding box; detecting video frames by using the model, and extracting the position, orientation and size of each frame of sperm; a multi-dimensional fusion scoring system is constructed through position, direction, speed, color and track continuity, adjacent frame targets are matched, a comprehensive score is generated, the targets are associated, and a continuous track and a unique identifier are formed and used for calculating motion parameters; and judging whether the track is static or low active according to the speed and displacement of the latest N frames, sealing the track to eliminate subsequent matching, and only reserving a non-sealed track to calculate motion parameters. According to the method, interference is effectively avoided, and the tracking accuracy and the reliability of sperm motility parameter calculation are remarkably improved.

The invention discloses an auxiliary reproductive semen treatment device matched with different insemination modes, which comprises a box body with an openable box door, a heating treatment table is arranged at the center of the bottom in the box body, and a treatment plate is placed on the heating treatment table; a control box is arranged at the top, a control circuit board is arranged in the control box, a heat dissipation plate and a display screen are arranged, and keys and knobs are arranged on the two sides; the back of the box is provided with a power interface. Through modular design, the device is compatible with semen treatment of various insemination modes such as IVF, ICSI and IUI; the first processing plate (transverse groove method) and the second processing plate (screen method) are used for screening sperms, through natural movement ability, form and thermotaxis guidance, mechanical damage is reduced, sperm motility is improved, sperm DNA fragment rate is reduced, and therefore ovum fertilization rate, embryo quality and pregnancy outcome are expected to be improved.

The invention relates to the technical field of livestock and poultry breeding, in particular to a goat hybridization breeding method capable of achieving complete-cycle nutrition enhancement. The core of the method is that systematic nutrition intervention runs through the whole breeding process. Before hybridization, performing differential nutrition pretreatment on the parents for 60 days: feeding the female parents with a breeding nutrition enhancer added with components such as D-chiro-inositol and N-acetylcysteine, and supplementing sperm motility enhancer containing acetylated L-carnitine and ergothioneine to the male parents. In the gamete treatment stage, the oocytes are subjected to in-vitro maturation culture in a special culture solution containing components such as D-chiro-inositol and a growth differentiation factor 9; after the sperms are subjected to gradient centrifugal screening, completing in-vitro fertilization in an optimized fertilization culture solution; after the embryos develop into blastocysts in the sequential culture system, carrying out laparoscopic transplantation, and carrying out staged precise nutrition culture on the pregnant ewes. According to the method, through multi-level nutrition enhancement, the reproductive capacity of the goats and the growth performance of offspring are effectively improved.

The application provides a sperm automatic detection and tracking method, device and storage medium. The method first pre-processes a sperm image, performs head-tail rotation boundary box labeling, and generates a training set containing center point coordinates, width, height and angle; a target detection model is trained using the training set to obtain a model capable of automatically identifying sperm and outputting a rotation boundary box; the model is applied to detect video frames to extract the position, orientation and size of each frame of sperm; a multi-dimensional fusion scoring system is constructed through position, direction, speed, color and trajectory continuity to match adjacent frames of targets, generate a comprehensive score and associate the targets to form a continuous trajectory and a unique identifier for calculating motion parameters; and then whether the trajectory is stationary or low active is judged according to the speed and displacement of the last N frames, the trajectory is sealed to exclude subsequent matching, and only the non-sealed trajectory is retained to calculate the motion parameters. The method effectively avoids interference, significantly improves the tracking accuracy and the reliability of sperm motility parameter calculation.

The invention discloses a method for predicting the sperm motility of a breeding bull based on a host SNP-rumen microorganism-metabolite interaction axis, and the sperm motility of the breeding bull can be obtained by detecting the haplotype condition of SNP1-SNP3 and / or SNP4-SNP6 sites of a breeding bull genome. The method breaks through the limitations of strong dependency on phenotype endpoint evaluation and prediction lag in the current bull seed selection process, and realizes forward accurate prediction and early evaluation of the semen quality of the breeding bull.

The invention relates to the technical field of semen preservative preparation, in particular to a diluent preparation capable of prolonging the normal-temperature survival time of boar semen and a preparation method of the diluent preparation. Glucose, L-glutamine, an antioxidant and polysaccharide components are scientifically proportioned, and an innovative polyethyleneimine-polysaccharide copolymer is combined, so that the normal-temperature survival time of boar semen is prolonged; the dual effects of sperm metabolism regulation and cell membrane protection are realized. The polyethyleneimine-polysaccharide copolymer enhances the biocompatibility and protective ability of the diluent through stable cross-linking of amino and carboxyl, and effectively relieves the problem of sudden change of osmotic pressure and acidity of the traditional preservative. In the formula, antioxidant components, such as reduced glutathione, vitamin E and coenzyme Q10, synergistically reduce oxidative stress and delay sperm senescence. According to the whole formula, proper pH and osmotic pressure are guaranteed, the sperm motility and survival time are remarkably prolonged, the requirement of pig industry for long-term preservation of sperm is met, and the feed additive has remarkable application value and popularization potential.

The invention discloses a yak frozen semen freezing process intelligent control method based on dynamic cooling curve optimization, and relates to the field of animal frozen semen preservation, and the yak frozen semen freezing process intelligent control method comprises the following steps: arranging a plurality of frozen semen tubes filled with consumed cattle semen on an annular tray with a cold source in the center in a radioactive manner, and uniformly arranging the plurality of frozen semen tubes in a surrounding manner in each radial distance interval; in the freezing process, cold energy is output according to a preset cooling program, and the annular tray is driven to rotate; unfreezing operation is executed, sperm motility values are measured, interval motility values of the sperm motility values of the frozen sperm tubules in all the radial distance intervals are counted, a temperature curve at the radial distance interval with the highest interval motility value is determined, a cooling program is modified in multiple rounds of experiments, and the process is repeated, so that the temperature curve is determined. And clustering the obtained temperature curves and selecting an optimal target temperature curve to perform freezing control. According to the method, the problem that the optimal dynamic cooling curve is difficult to efficiently obtain in the freezing process of the frozen yak sperms is solved.

The invention relates to the technical field of image processing and reproductive hospital inspection, and provides a sperm movement tracking and quality analysis method based on deep learning and optical flow fusion, which comprises the following steps of: identifying sperms through a target detection model, acquiring a detection frame, and screening to obtain detection points; predicting the predicted position of the sperm in the current frame by using a sparse optical flow method based on the historical trajectory, performing data association on the predicted position and a detection point, establishing a matching relationship, updating the trajectory according to the matching relationship, establishing a new trajectory or terminating a lost trajectory, calculating kinematics parameters and sperm concentration based on the complete motion trajectory, and completing activity grading. According to the invention, through fusion of deep learning detection and optical flow prediction, high-precision identification under a complex background and stable tracking under a high-density cross scene are realized, the identity exchange rate is significantly reduced, and the shielding robustness is enhanced; and meanwhile, full-process automation from video input to clinical report is realized, and the accuracy, repeatability and clinical credibility of an analysis result are improved.

This invention discloses a pharmaceutical composition for treating asthenospermia by activating the Nrf2 / HO-1 / GPx4 pathway and its application. The pharmaceutical composition comprises taurine and geranium as active ingredients, wherein the mass ratio of taurine to geranium is 3-5:1. The pharmaceutical composition of this invention can synergistically activate the Nrf2 / HO-1 / GPx4 antioxidant signaling pathway, upregulate the expression of downstream antioxidant enzymes GPx4 and HO-1, inhibit lipid peroxidation, protect sperm mitochondrial function, and simultaneously improve the microenvironmental metabolic profile of the epididymis—the site of sperm maturation—thereby enhancing sperm motility. The pharmaceutical composition provided by this invention can be used to prepare a treatment for asthenospermia, particularly oxidative stress-induced asthenospermia.

The invention is applicable to the technical field of animal genetic engineering, and provides a DHX36 gene molecular marker related to bovine sperm motility traits and application thereof, the DHX36 gene molecular marker is a 231st locus of a bovine DHX36 gene segment as shown in a sequence table SEQ ID NO: 1, and the locus has a C-G base mutation. Based on the DHX36 gene molecular marker, the genotype is judged through sequence determination, and the genotype can be associated with the normal and unfrozen activity traits of bovine sperms. An analysis result shows that the bovine sperms of individuals with different genotypes are normal and have significant differences in vitality after unfreezing. According to the invention, the specific primer group is utilized to obtain the bovine DHX36 gene segment related to the normal and unfrozen activity traits of bovine sperms, and the specific SNP site in the segment is taken as the molecular marker, so that a theoretical basis and specific application are provided for marker-assisted selection of cattle.

The invention relates to a protective liquid formula for improving the survival rate of frozen and thawed sperms of pigs. The protective liquid formula comprises the following components in percentage by mass: 14% of sodium hexadecylsulfonate, 7% of coconut oil monoethanolamide and 79% of distilled water. The preparation method comprises the following steps: 1) weighing 14g of sodium hexadecylsulfonate and 7g of coconut oil monoethanolamide, and dissolving in 79g of distilled water; and 2) uniformly stirring the solution obtained in the step 1) at 60 DEG C until the sodium hexadecyl sulfonate and the coconut oil monoethanolamide are completely dissolved in the distilled water. The protection liquid has the advantages that the protection liquid protects porcine sperm cells, provides a suitable living environment for porcine sperms, improves the oxidative stress resistance and the preservation effect of the porcine sperms, ensures the survival rate of the porcine sperms, and further improves the breeding rate and the breeding benefits of sows; the survival rate, the sperm motility and the acrosome integrity rate of the frozen and thawed sperms of the pigs are obviously improved; the feed additive is simple in proportioning, high in reliability, low in economic cost, high in biological value and convenient to popularize and apply in production, and yolk with extremely high preparation requirements is not used.

The invention relates to the technical field of lion-head goose breeding, in particular to a method for screening and identifying genes related to lion-head goose sperm motility regulation and control, which comprises the following steps: dividing healthy lion-head male geese in a breeding period into a high sperm motility group and a low sperm motility group according to sperm motility, collecting two groups of testicular tissues and sperm samples, and preserving the testicular tissues at low temperature for molecular detection; performing transcriptome sequencing on the testicular tissue to obtain mRNA and non-coding RNA expression data; screening two groups of nucleic acid molecules with significant differential expression, and determining candidate genes through functional enrichment analysis of reproductive development related biological pathways; and verifying the authenticity of the expression difference of the candidate gene through fluorescent quantitative PCR, and finally determining the target gene. According to the method, related data are obtained through sequencing, the candidate genes are determined by screening differential molecules through enrichment analysis and PCR verification, accurate identification of a related gene system is achieved, and the problems of missing detection and insufficient accuracy caused by lack of transcriptome sequencing in a traditional method are solved.

The application discloses a Holstein cow sperm activity miRNA molecular marker selection method, which comprises the following steps: (1) specific primers are designed according to a Holstein cow miRNA precursor region, and PCR amplification is carried out on to-be-detected cow genomic DNA to obtain an amplification product; (2) the PCR product is subjected to enzyme cutting by using a BspE I restriction endonuclease, and electrophoresis typing is carried out, and three genotypes, namely, a TT type, a TC type and a CC type, are obtained; and (3) the sperm activity of the CC type is significantly higher than that of the TT type, and the sperm deformity rate of the CC type is significantly lower than that of the TT type, so the CC type and the TC type are selected for breeding, and the C genotype frequency is improved. The method can improve the accuracy of sperm quality selection of the cow, is used for the selection and breeding of high-quality cows, and provides a selection and breeding tool for strengthening the excellent germplasm of Chinese Holstein cows.

The present application relates to the field of animal breeding technology, in particular to the application of ursolic acid in the frozen semen preservation of sheep. The present application applies ursolic acid in the frozen semen preservation of sheep. The addition of 0.5-2.0 micromole / L ursolic acid in the frozen diluent can improve the quality of frozen semen preservation of sheep and goats, and can improve the sperm motility and vitality after thawing, which helps the thawed sperm to pass through the vagina, uterus and enter the oviduct to complete fertilization, improves the insemination success rate of frozen sperm, and provides technical support for strengthening the protection of local sheep breed resources.

A sperm primary screening optimization device without vacuum driving is characterized in that the device comprises a sperm taking cup, a sampling test tube, a microflow connecting channel, a negative pressure device and a connecting cover, the connecting cover is connected with the sampling test tube, the negative pressure device is connected with the connecting cover, and an adjusting valve is arranged on the negative pressure device; the sperm collecting cup is connected with the sampling test tube through the micro-flow connecting channel, the filter screen is arranged on the micro-flow connecting channel, and the cup cover is arranged on the sperm collecting cup. Micro negative pressure generated by the negative pressure device can provide mild driving force for flowing of semen, meanwhile, the flow speed of sperms is effectively controlled, and the sperm motility is prevented from being damaged by high-speed flowing; impurities such as seminal plasma gel and the like can be effectively blocked by arranging the filter screen, and the active sperms can penetrate through the filter screen more easily by utilizing the limitation of the aperture of the meshes, so that physical compression on the sperms is avoided, and the integrity of the active sperms is protected.

The invention relates to the technical field of animal feeds, in particular to a mouse feed and a preparation method and application thereof, and the mouse feed comprises curcumin and a basic ration; wherein the curcumin is provided in the form of a drug-loaded pellet; the basic ration is SPF (specific pathogen free) grade rat ration. The feed for the rats contains curcumin, effectively relieves damage of the plateau low-oxygen environment to the reproductive system of the rats, improves sperm motility, reduces the malformation rate, adjusts the reproductive hormone level, and provides an innovative solution for plateau animal reproductive health management.

The invention relates to the technical field of biological detection equipment, and discloses a rapid sperm motility detection device and a detection method thereof.The rapid sperm motility detection device comprises a base, a microscope and a heat preservation box, a heat preservation groove is formed in the heat preservation box, and a clamp, a driving assembly and a cover plate are arranged above the heat preservation box. The sperm storage bottle is fixed through the clamp, then the sperm storage bottle is immersed into the heat preservation tank through the driving assembly to achieve liquefaction, the driving assembly can drive the sperm storage bottle to intermittently and temporarily incline in the heat preservation tank, and at the moment, the liquefied sperms can be monitored through the water immersion sensor; compared with manual observation of the liquidation degree, the sperm storage bottle is more accurate, frequent taking out of the sperm storage bottle from the heat preservation tank is avoided, the stability of the liquidation environment is ensured, the sperms can be taken out at the first time after liquidation is completed, the liquidation time is prevented from being too long or too short, and the sperm storage bottle can be moved out of the heat preservation tank. And the accuracy of subsequent sperm motility detection is improved.

The present application provides a symbiont engineered to express an antibody fragment that inhibits sperm motility or fertilization, and the like. The present application provides use of the engineered symbiont or the composition containing the same as an effective contraceptive method for a female.

The utility model aims to provide an auxiliary device for semen dilution, and relates to the technical field of medical instruments, a dilution electric push rod is fixedly arranged in a box body, a dilution arm is driven to move downwards through the contraction of the dilution electric push rod so as to drive a dilution guide pipe to be close to a semen taking cup, and a constant delivery pump is arranged on a dilution box; the diluent in the dilution box is quantitatively controlled to be added into the semen taking cup, so that the addition of the diluent is accurately controlled; a stirring electric push rod is fixedly arranged in the box body, a stirring motor drives a blowing rod to stir semen in a semen taking cup, the stirring range of the blowing rod is adjusted through sliding of a fixing head, and air flow is conveyed to the blowing rod through an air pump for blowing, so that the semen is automatically stirred, blown and beaten, and the semen dilution efficiency and effect are improved; the heater is arranged in the dilution box and is matched with the heating base, so that the temperature of the semen and the diluent in the semen taking cup is kept at about 37 DEG C, the temperature difference is reduced, and the sperm motility after the semen is diluted is well kept.

The invention discloses a low-density sperm braking method without a PVP medium in ICSI fertilization, and relates to the field of assisted reproduction therapy, and the method comprises the following steps: step 1, diluting a sperm suspension until the density is 0.1 * 10 < 6 > / ml, and placing the diluted sperm suspension in an in-vitro operating solution without PVP; step 2, under an inverted microscope, directly pressing and braking the tail of the sperm in the in-vitro operating fluid by using a microinjection needle, so that the tail of the sperm is folded or curled to deform; and step 3, transferring the braked sperms into operation liquid drops containing ovum to complete microinjection, and performing tail pressing operation in an isotonic operation liquid without PVP (Polyvinyl Pyrrolidone), so that the tail parts of the sperms present visible curled deformation or tail folding. The sperm motility destruction rate is 100% (residual motility is completely prevented from interfering fertilization), and the tail cell membrane rupture rate is 100% (it is ensured that activation factors in sperms are fully released).

The application belongs to the field of biological medicine, and particularly relates to a kind of Bungarus multicinctus venom protein and a preparation method and application thereof.The application provides a new sperm motility promoter, which is derived from Bungarus multicinctus venom and has the application potential to be developed into a new sperm motility promoter.

The present application provides use of compounds represented by formula (I) and formula (II) in the preparation of drugs for improving male reproductive ability. In the present application, different oligoasthenospermia models are constructed, and the improvement effects of the compounds represented by formula (I) and formula (II) on male reproductive ability impairment are verified by means of sperm count analysis, sperm motility analysis, mRNA expression level analysis of inflammatory and antioxidant genes, testicular histopathological damage analysis, etc. The experimental results show that the compounds represented by formula (I) and formula (II) increase the sperm count in mice with oligoasthenospermia, improve the sperm motility of the mice with oligoasthenospermia, ameliorate testicular histopathological damage in the mice, suppress the expression of inflammatory factors, and increase the expression of antioxidant genes, demonstrating great potential in the treatment of oligoasthenospermia.

This invention belongs to the field of animal husbandry technology and relates to a method for improving the quality of frozen semen from Lanzhou fat-tailed sheep based on ergothioneine. This application, through optimized combination of diluent, 8 layers of gauze wrapping for cooling, 4 cm fumigation height, and 8 mM ergothioneine, achieved a sperm motility rate of 45.80±3.62%, an acrosome integrity rate of 52.32±2.28%, and a plasma membrane integrity rate of 55.38±3.55% after thawing. All indicators are significantly better than those of the traditional single-optimization group.

The invention relates to the technical field of aquaculture, and discloses a method for manufacturing and transplanting artificial spermatophore of Xinxiu lobsters. The method comprises the following steps: acquiring a physiological and biochemical parameter set, including testis development stage indexes, sperm motility density values and spermatophore matrix component proportions, of the Xinxiu lobsters to be treated; constructing a multi-modal spermatophore quality evaluation link, performing fusion analysis on the multi-source spermatophore feature flow, and determining artificial spermatophore simulation parameters and receptor lobster adaptation parameters; a dynamic environment simulation device is arranged, and a multi-dimensional environment disturbance signal is generated according to the adaptive parameters to simulate transplantation environment fluctuation; establishing a distributed operation framework, and generating a node operation strategy based on a transplantation control node state and environment disturbance; generating a spermatophore transplantation operation instruction based on the simulation parameters; and creating an encrypted anti-interference operation channel, and implementing node cooperative processing and artificial spermatophore transplantation execution. According to the method, a multi-link technology is integrated, and a systematic scheme is provided for making and transplanting the artificial spermatophore of the Xinxiu lobsters.

The invention discloses a plant lactobacillus, a metagen and application of the plant lactobacillus and the metagen for improving the activity and quality of male sperms, the plant lactobacillus is plant lactobacillus BN04, the plant lactobacillus is preserved in China General Microbiological Culture Collection Center (CGMCC) on November 22, 2023, and the preservation number is CGMCC No.29102. The invention further discloses a preparation method of the plant lactobacillus and the metagen for improving the activity and quality of male sperms and application of the plant lactobacillus and the metagen for improving the activity and quality of male sperms. The plant lactobacillus BN04 provided by the invention can regulate the copper content in seminal plasma to a normal level and improve the sperm motility and quality, and the metagen prepared from the plant lactobacillus BN04 can enhance the acrosin activity of sperms and improve the sperm quality.

This invention discloses a method for cryopreservation of donkey semen using antioxidants, belonging to the field of animal reproductive technology. The method is based on a specific basal diluent, with the addition of targeted mitochondrial antioxidants SKQ1 or SS-31. First, a basal diluent containing sugars, citrate, proline, skim milk powder, and antibiotics is prepared. Then, SKQ1 (dissolved in DMSO) or SS-31 (dissolved in physiological saline) is separately prepared into a stock solution, which is then mixed with the basal diluent to obtain a composite preservation solution. Finally, the donkey semen treated with this composite preservation solution is diluted and stored at 4°C. The method of this invention can significantly improve sperm motility and acrosome integrity rate of donkey semen stored at 4°C for 5 days, reduce ROS content and abnormality rate, and significantly increase the insemination and conception rate of the preserved semen to over 40%, effectively extending the practical preservation period of donkey semen.

The invention discloses a large yellow croaker semen super-long-time preservation and liquid nitrogen re-freezing method based on a dry ice medium, and solves the problems that existing liquid nitrogen preservation and transportation are limited, dry ice preservation time is short, and liquid nitrogen long-term preservation cannot be linked. The method comprises the following steps: firstly, mixing seminal fluid with a sperm diluent containing specific components and a cryopreservation solution in proportion, balancing at 4 DEG C for 15 minutes, sub-packaging and embedding into dry ice for preservation; the cryopreserved seminal fluid can be directly recovered for use or put into liquid nitrogen for re-freezing for long-term preservation. According to the method provided by the invention, the sperm motility is still up to 90% or above after the sperm is preserved in dry ice for 72 hours, and the unfrozen sperm motility can still be kept at 80% or above after the sperm preserved in the dry ice is subjected to liquid nitrogen re-freezing preservation. According to the method, the difficult problem of safe and convenient ultra-long distance dry ice transportation of the seminal fluid is successfully solved, seamless connection of dry ice long-distance transportation and liquid nitrogen permanent preservation is realized for the first time, and a powerful technical support is provided for large yellow croaker cross-regional germplasm resource collection, preservation, improved variety breeding and industrial sustainable development.

The invention provides an assisted reproduction IVF outcome prediction model based on an LSTM and LSTM-CNN fusion architecture and a construction method, and is applied to the field of data processing. The method comprises the following steps: acquiring assisted reproduction clinical layering core data (including IVF period original data and divided into conventional fertilization IVF and ICSI fertilization IVF according to a fertilization mode), key parameters (including sperm motility and the like in conventional IVF and additional sperm morphological images in ICSI) and target patient information; preprocessing the data, carrying out feature engineering, and generating multi-modal feature vectors adaptive to the two fertilization modes; then, an optimization prediction model is constructed, and an initial model and performance evaluation information are obtained; iteratively optimizing the model, introducing a new module to improve the multi-modal learning ability, and generating an SOTA level model through multi-index evaluation; and finally, processing target patient information by using the model, and outputting a personalized prediction reference scheme and clinical application value analysis information.

The invention discloses a PID1 gene linked homozygous mutation SNP (Single Nucleotide Polymorphism) molecular marker related to sperm motility of drakes and application thereof, and belongs to the technical field of biology. The homozygous mutation SNP molecular marker comprises SNP1 and SNP2, the nucleotide sequence of the SNP1 is shown as SEQ ID NO.1, and T / C mutation exists at the 70th site of the sequence; the nucleotide sequence of the SNP2 is as shown in SEQ ID NO.2, and G / T mutation exists at the 49th site of the sequence. The sperm motility when the mutation site genotype combination of the SNP1 and the SNP2 is TG / TG is higher than the sperm motility when the mutation site genotype combination of the SNP1 and the SNP2 is TG / CT and CT / CT genotypes. The molecular marker disclosed by the invention can be applied to early selection of the breeding drakes, is beneficial to accelerating the breeding progress of the breeding drakes, improving the breeding accuracy and reducing the breeding cost and the production cost, and has great economic application value and breeding value for the breeding drakes.