34 results about "Sperm motility" patented technology

The invention relates to the technical field of livestock and poultry breeding, in particular to a goat hybridization breeding method capable of achieving complete-cycle nutrition enhancement. The core of the method is that systematic nutrition intervention runs through the whole breeding process. Before hybridization, performing differential nutrition pretreatment on the parents for 60 days: feeding the female parents with a breeding nutrition enhancer added with components such as D-chiro-inositol and N-acetylcysteine, and supplementing sperm motility enhancer containing acetylated L-carnitine and ergothioneine to the male parents. In the gamete treatment stage, the oocytes are subjected to in-vitro maturation culture in a special culture solution containing components such as D-chiro-inositol and a growth differentiation factor 9; after the sperms are subjected to gradient centrifugal screening, completing in-vitro fertilization in an optimized fertilization culture solution; after the embryos develop into blastocysts in the sequential culture system, carrying out laparoscopic transplantation, and carrying out staged precise nutrition culture on the pregnant ewes. According to the method, through multi-level nutrition enhancement, the reproductive capacity of the goats and the growth performance of offspring are effectively improved.

The application provides a sperm automatic detection and tracking method, device and storage medium. The method first pre-processes a sperm image, performs head-tail rotation boundary box labeling, and generates a training set containing center point coordinates, width, height and angle; a target detection model is trained using the training set to obtain a model capable of automatically identifying sperm and outputting a rotation boundary box; the model is applied to detect video frames to extract the position, orientation and size of each frame of sperm; a multi-dimensional fusion scoring system is constructed through position, direction, speed, color and trajectory continuity to match adjacent frames of targets, generate a comprehensive score and associate the targets to form a continuous trajectory and a unique identifier for calculating motion parameters; and then whether the trajectory is stationary or low active is judged according to the speed and displacement of the last N frames, the trajectory is sealed to exclude subsequent matching, and only the non-sealed trajectory is retained to calculate the motion parameters. The method effectively avoids interference, significantly improves the tracking accuracy and the reliability of sperm motility parameter calculation.

The invention discloses a yak frozen semen freezing process intelligent control method based on dynamic cooling curve optimization, and relates to the field of animal frozen semen preservation, and the yak frozen semen freezing process intelligent control method comprises the following steps: arranging a plurality of frozen semen tubes filled with consumed cattle semen on an annular tray with a cold source in the center in a radioactive manner, and uniformly arranging the plurality of frozen semen tubes in a surrounding manner in each radial distance interval; in the freezing process, cold energy is output according to a preset cooling program, and the annular tray is driven to rotate; unfreezing operation is executed, sperm motility values are measured, interval motility values of the sperm motility values of the frozen sperm tubules in all the radial distance intervals are counted, a temperature curve at the radial distance interval with the highest interval motility value is determined, a cooling program is modified in multiple rounds of experiments, and the process is repeated, so that the temperature curve is determined. And clustering the obtained temperature curves and selecting an optimal target temperature curve to perform freezing control. According to the method, the problem that the optimal dynamic cooling curve is difficult to efficiently obtain in the freezing process of the frozen yak sperms is solved.

The invention relates to the technical field of image processing and reproductive hospital inspection, and provides a sperm movement tracking and quality analysis method based on deep learning and optical flow fusion, which comprises the following steps of: identifying sperms through a target detection model, acquiring a detection frame, and screening to obtain detection points; predicting the predicted position of the sperm in the current frame by using a sparse optical flow method based on the historical trajectory, performing data association on the predicted position and a detection point, establishing a matching relationship, updating the trajectory according to the matching relationship, establishing a new trajectory or terminating a lost trajectory, calculating kinematics parameters and sperm concentration based on the complete motion trajectory, and completing activity grading. According to the invention, through fusion of deep learning detection and optical flow prediction, high-precision identification under a complex background and stable tracking under a high-density cross scene are realized, the identity exchange rate is significantly reduced, and the shielding robustness is enhanced; and meanwhile, full-process automation from video input to clinical report is realized, and the accuracy, repeatability and clinical credibility of an analysis result are improved.

This invention discloses a pharmaceutical composition for treating asthenospermia by activating the Nrf2 / HO-1 / GPx4 pathway and its application. The pharmaceutical composition comprises taurine and geranium as active ingredients, wherein the mass ratio of taurine to geranium is 3-5:1. The pharmaceutical composition of this invention can synergistically activate the Nrf2 / HO-1 / GPx4 antioxidant signaling pathway, upregulate the expression of downstream antioxidant enzymes GPx4 and HO-1, inhibit lipid peroxidation, protect sperm mitochondrial function, and simultaneously improve the microenvironmental metabolic profile of the epididymis—the site of sperm maturation—thereby enhancing sperm motility. The pharmaceutical composition provided by this invention can be used to prepare a treatment for asthenospermia, particularly oxidative stress-induced asthenospermia.

The invention is applicable to the technical field of animal genetic engineering, and provides a DHX36 gene molecular marker related to bovine sperm motility traits and application thereof, the DHX36 gene molecular marker is a 231st locus of a bovine DHX36 gene segment as shown in a sequence table SEQ ID NO: 1, and the locus has a C-G base mutation. Based on the DHX36 gene molecular marker, the genotype is judged through sequence determination, and the genotype can be associated with the normal and unfrozen activity traits of bovine sperms. An analysis result shows that the bovine sperms of individuals with different genotypes are normal and have significant differences in vitality after unfreezing. According to the invention, the specific primer group is utilized to obtain the bovine DHX36 gene segment related to the normal and unfrozen activity traits of bovine sperms, and the specific SNP site in the segment is taken as the molecular marker, so that a theoretical basis and specific application are provided for marker-assisted selection of cattle.

The invention relates to the technical field of lion-head goose breeding, in particular to a method for screening and identifying genes related to lion-head goose sperm motility regulation and control, which comprises the following steps: dividing healthy lion-head male geese in a breeding period into a high sperm motility group and a low sperm motility group according to sperm motility, collecting two groups of testicular tissues and sperm samples, and preserving the testicular tissues at low temperature for molecular detection; performing transcriptome sequencing on the testicular tissue to obtain mRNA and non-coding RNA expression data; screening two groups of nucleic acid molecules with significant differential expression, and determining candidate genes through functional enrichment analysis of reproductive development related biological pathways; and verifying the authenticity of the expression difference of the candidate gene through fluorescent quantitative PCR, and finally determining the target gene. According to the method, related data are obtained through sequencing, the candidate genes are determined by screening differential molecules through enrichment analysis and PCR verification, accurate identification of a related gene system is achieved, and the problems of missing detection and insufficient accuracy caused by lack of transcriptome sequencing in a traditional method are solved.

The application discloses a Holstein cow sperm activity miRNA molecular marker selection method, which comprises the following steps: (1) specific primers are designed according to a Holstein cow miRNA precursor region, and PCR amplification is carried out on to-be-detected cow genomic DNA to obtain an amplification product; (2) the PCR product is subjected to enzyme cutting by using a BspE I restriction endonuclease, and electrophoresis typing is carried out, and three genotypes, namely, a TT type, a TC type and a CC type, are obtained; and (3) the sperm activity of the CC type is significantly higher than that of the TT type, and the sperm deformity rate of the CC type is significantly lower than that of the TT type, so the CC type and the TC type are selected for breeding, and the C genotype frequency is improved. The method can improve the accuracy of sperm quality selection of the cow, is used for the selection and breeding of high-quality cows, and provides a selection and breeding tool for strengthening the excellent germplasm of Chinese Holstein cows.

The present application relates to the field of animal breeding technology, in particular to the application of ursolic acid in the frozen semen preservation of sheep. The present application applies ursolic acid in the frozen semen preservation of sheep. The addition of 0.5-2.0 micromole / L ursolic acid in the frozen diluent can improve the quality of frozen semen preservation of sheep and goats, and can improve the sperm motility and vitality after thawing, which helps the thawed sperm to pass through the vagina, uterus and enter the oviduct to complete fertilization, improves the insemination success rate of frozen sperm, and provides technical support for strengthening the protection of local sheep breed resources.

The invention discloses a low-density sperm braking method without a PVP medium in ICSI fertilization, and relates to the field of assisted reproduction therapy, and the method comprises the following steps: step 1, diluting a sperm suspension until the density is 0.1 * 10 < 6 > / ml, and placing the diluted sperm suspension in an in-vitro operating solution without PVP; step 2, under an inverted microscope, directly pressing and braking the tail of the sperm in the in-vitro operating fluid by using a microinjection needle, so that the tail of the sperm is folded or curled to deform; and step 3, transferring the braked sperms into operation liquid drops containing ovum to complete microinjection, and performing tail pressing operation in an isotonic operation liquid without PVP (Polyvinyl Pyrrolidone), so that the tail parts of the sperms present visible curled deformation or tail folding. The sperm motility destruction rate is 100% (residual motility is completely prevented from interfering fertilization), and the tail cell membrane rupture rate is 100% (it is ensured that activation factors in sperms are fully released).

The application belongs to the field of biological medicine, and particularly relates to a kind of Bungarus multicinctus venom protein and a preparation method and application thereof.The application provides a new sperm motility promoter, which is derived from Bungarus multicinctus venom and has the application potential to be developed into a new sperm motility promoter.

The present application provides use of compounds represented by formula (I) and formula (II) in the preparation of drugs for improving male reproductive ability. In the present application, different oligoasthenospermia models are constructed, and the improvement effects of the compounds represented by formula (I) and formula (II) on male reproductive ability impairment are verified by means of sperm count analysis, sperm motility analysis, mRNA expression level analysis of inflammatory and antioxidant genes, testicular histopathological damage analysis, etc. The experimental results show that the compounds represented by formula (I) and formula (II) increase the sperm count in mice with oligoasthenospermia, improve the sperm motility of the mice with oligoasthenospermia, ameliorate testicular histopathological damage in the mice, suppress the expression of inflammatory factors, and increase the expression of antioxidant genes, demonstrating great potential in the treatment of oligoasthenospermia.

This invention belongs to the field of animal husbandry technology and relates to a method for improving the quality of frozen semen from Lanzhou fat-tailed sheep based on ergothioneine. This application, through optimized combination of diluent, 8 layers of gauze wrapping for cooling, 4 cm fumigation height, and 8 mM ergothioneine, achieved a sperm motility rate of 45.80±3.62%, an acrosome integrity rate of 52.32±2.28%, and a plasma membrane integrity rate of 55.38±3.55% after thawing. All indicators are significantly better than those of the traditional single-optimization group.

This invention discloses a method for cryopreservation of donkey semen using antioxidants, belonging to the field of animal reproductive technology. The method is based on a specific basal diluent, with the addition of targeted mitochondrial antioxidants SKQ1 or SS-31. First, a basal diluent containing sugars, citrate, proline, skim milk powder, and antibiotics is prepared. Then, SKQ1 (dissolved in DMSO) or SS-31 (dissolved in physiological saline) is separately prepared into a stock solution, which is then mixed with the basal diluent to obtain a composite preservation solution. Finally, the donkey semen treated with this composite preservation solution is diluted and stored at 4°C. The method of this invention can significantly improve sperm motility and acrosome integrity rate of donkey semen stored at 4°C for 5 days, reduce ROS content and abnormality rate, and significantly increase the insemination and conception rate of the preserved semen to over 40%, effectively extending the practical preservation period of donkey semen.

The invention provides an assisted reproduction IVF outcome prediction model based on an LSTM and LSTM-CNN fusion architecture and a construction method, and is applied to the field of data processing. The method comprises the following steps: acquiring assisted reproduction clinical layering core data (including IVF period original data and divided into conventional fertilization IVF and ICSI fertilization IVF according to a fertilization mode), key parameters (including sperm motility and the like in conventional IVF and additional sperm morphological images in ICSI) and target patient information; preprocessing the data, carrying out feature engineering, and generating multi-modal feature vectors adaptive to the two fertilization modes; then, an optimization prediction model is constructed, and an initial model and performance evaluation information are obtained; iteratively optimizing the model, introducing a new module to improve the multi-modal learning ability, and generating an SOTA level model through multi-index evaluation; and finally, processing target patient information by using the model, and outputting a personalized prediction reference scheme and clinical application value analysis information.

The invention discloses a PID1 gene linked homozygous mutation SNP (Single Nucleotide Polymorphism) molecular marker related to sperm motility of drakes and application thereof, and belongs to the technical field of biology. The homozygous mutation SNP molecular marker comprises SNP1 and SNP2, the nucleotide sequence of the SNP1 is shown as SEQ ID NO.1, and T / C mutation exists at the 70th site of the sequence; the nucleotide sequence of the SNP2 is as shown in SEQ ID NO.2, and G / T mutation exists at the 49th site of the sequence. The sperm motility when the mutation site genotype combination of the SNP1 and the SNP2 is TG / TG is higher than the sperm motility when the mutation site genotype combination of the SNP1 and the SNP2 is TG / CT and CT / CT genotypes. The molecular marker disclosed by the invention can be applied to early selection of the breeding drakes, is beneficial to accelerating the breeding progress of the breeding drakes, improving the breeding accuracy and reducing the breeding cost and the production cost, and has great economic application value and breeding value for the breeding drakes.

The invention relates to the technical field of medicines, and discloses an application of lupin or an extract thereof in preparation of a drug for relieving reproductive toxicity of chemotherapeutic drugs, the lupin is selected from lupin plants, preferably lupin variety Jenabillup, and the chemotherapeutic drugs comprise busulfan, cyclophosphamide or cis-platinum. The administration dosage of the lupin or the extract thereof is 0.1-100 mg / kg body weight, the administration period is continuous administration for at least 5 weeks, experiments show that the lupin can significantly improve the sperm concentration and sperm motility of chemotherapy model animals and improve pathological damage of testicular tissues, the mechanism relates to antioxidant, anti-inflammatory and anti-apoptosis effects, and the lupin or the extract thereof can be used for preparing a chemotherapy model animal. The invention provides a safe and effective new strategy for fertility protection of chemotherapy patients.

The invention relates to the technical field of livestock breeding, in particular to a special lick brick for relieving heat stress and improving bull semen quality as well as a preparation method and application of the special lick brick. According to the special lick brick, on the premise that basic mineral supply is not changed, through synergistic addition of astaxanthin and betaine, key semen quality indexes such as the number of sperms, sperm motility and the like of Holstein breeding bulls advancing linearly are pertinently improved. Meanwhile, through the synergistic effect of astaxanthin and betaine, the oxidation resistance of the body can be remarkably enhanced, the heat stress related index level is reduced, and inhibition of heat stress on reproductive performance is relieved. The preparation method provided by the invention is simple and convenient in process, convenient to use and controllable in cost, not only can meet the requirement of large-scale breeding for practicability, but also can indirectly improve the breeding economic benefit by improving the semen quality, is suitable for daily breeding of 1-4-year-old Holstein breeding bulls, and has wide popularization and application prospects.

The present application relates to biological detection equipment technical field, disclose a kind of rapid detection device and detection method of sperm motility, including base, microscope and incubator, the incubator is provided with incubation tank, the incubator top is provided with fixture, drive assembly and cover plate.The present application is fixed sperm storage bottle by fixture, then sperm storage bottle is immersed into incubation tank using drive assembly, liquefaction can be realized, and drive assembly can drive sperm storage bottle to be intermittently and briefly inclined inside incubation tank, water immersion sensor can be used to monitor sperm at this time Liquefaction is completed, and drive assembly is used again to move sperm storage bottle to outside incubation tank, compared with artificial observation liquefaction degree, the present application is more accurate, and the present application avoids frequently taking sperm storage bottle from incubation tank, ensures the stability of liquefaction environment, and also can take out sperm in the first time after liquefaction, avoid liquefaction time too long or too short, improve the accuracy of subsequent sperm motility detection.

The invention belongs to the technical field of biology. The invention relates to an antioxidant composition, in particular to an antioxidant composition and application thereof in normal-temperature preservation of boar semen. Researches find that the activity of boar sperms in the normal-temperature preservation period can be effectively improved by independently adding lycopene or antioxidant SKQ1, the combined use effect of lycopene and antioxidant SKQ1 shows a synergistic effect, and when the boar sperms are preserved to the seventh day, the average sperm activity value exceeds 70%. In addition, through combined treatment, the integrity of sperm acrosome and plasma membrane is remarkably maintained, the antioxidant enzyme activity of sperms is improved, the active oxygen and malonaldehyde level are effectively reduced, meanwhile, the mitochondrial membrane potential and the adenosine triphosphate content are remarkably improved, and the expression level of sperm P-AMPK and Nrf2 protein is improved. In conclusion, the lycopene and the SKQ1 are combined for use, oxidative stress and energy metabolism are synergistically regulated and controlled, the quality and function stability of the boar sperms in the normal-temperature preservation process are remarkably improved, and the lycopene and the SKQ1 have good application value and application prospects.

The invention relates to the field of boar semen preservation, in particular to preparation and application of a ferulic acid-based boar semen low-temperature diluent. The proportion of the ferulic acid in the basic diluent is 0.2 to 0.8 mmol / L; wherein the basic diluent is prepared by dissolving 3.00 g of glucose, 4.00 g of cane sugar, 0.03 g of gentamicin sulfate and 20 mL of yolk in 80 mL of double distilled water, uniformly stirring, centrifuging at 4 DEG C and 7000 * g for 25 min, adjusting the pH value of the solution to 6.80-7.00, and adjusting the osmotic pressure of the solution to 290-300 mOsm / kg. The method has the beneficial effects that ferulic acid is used as a protective agent for preserving the boar semen at 4 DEG C for the first time, the effect is reliable, popularization is easy, the preserved boar semen is suitable for artificial insemination and artificial insemination after long-distance transportation, the motility, motility rate, acrosome integrity rate and plasma membrane integrity rate of boar sperms preserved at 4 DEG C can be remarkably improved, and the fertilization activity of the sperms is kept.

This invention discloses a dynamic screening and trajectory tracking method and system for accurate assessment of sperm motility, relating to the interdisciplinary fields of medical image analysis and artificial intelligence. This invention aims to address the problems of high subjectivity and low tracking accuracy in existing sperm motility analysis methods. The system consists of a preprocessing module, a detection module, a preheating screening module, a locking and tracking module, and a visualization output module. It includes: acquiring and preprocessing sperm motility videos; constructing an improved YOLO11 model for sperm detection; generating temporary trajectories using the ByteTrack algorithm and calculating quantification parameters; constructing a weighted scoring model based on velocity, persistence, and confidence to dynamically screen out high-activity trajectories; and locking and tracking only high-activity sperm in subsequent frames. This invention overcomes the limitations of existing methods that rely on single velocity or morphology recognition, achieving accurate grading and dynamic tracking of sperm motility, and has clinical application value.

The present application provides a food composition for preventing or alleviating andropause syndrome, comprising fermented noni fermented with Lactobacillus plantarum NST1805, and a preparation method therefor. More specifically, the present application provides a food composition for preventing or alleviating andropause syndrome and a preparation method therefor, the food composition being capable of efficiently preventing or alleviating andropause syndrome through regulation of male hormone production and / or improvement of sperm motility.

This invention discloses a fertility assessment method and system based on sperm motility trajectory coordinates, belonging to the field of medical informatics technology. The assessment method specifically involves: acquiring multiple consecutive frames of sperm motility images from a sperm motility video, and extracting the head center coordinates and tail center coordinates of each target sperm in each frame of the sperm motility image, thereby determining the trajectory sequence corresponding to each target sperm; based on the standardized trajectory sequence corresponding to each target sperm, using an unsupervised machine learning clustering algorithm to divide all target sperm into multiple motility pattern subgroups, further determining the sperm motility characteristics of each motility pattern subgroup, and generating fertility assessment results for each motility pattern subgroup. This invention uses the complete sperm motility trajectory sequence as the direct basis for cluster analysis, which can accurately capture the subtle and complex potential differences in sperm motility patterns, achieving refined subgrouping of sperm motility patterns.

The invention relates to the field of biological detection and toxicological evaluation, and discloses an in-vitro detection method for screening or evaluating sperm motility toxicity of a compound. The method is realized by detecting a group of specific biomarkers in mammalian sperm treated by a tested compound, and the marker combination comprises a phosphoserine / threonine PKA substrate level, an AKAP3 protein level and an AKAP4 protein level. Researches show that environmental toxin fenvalerate can specifically reduce the levels of the markers by destroying cAMP / PKA signal channel, so that sperm motility is reduced. Through quantitative analysis of changes of the markers, whether a tested compound has a similar sperm direct toxicity effect or not can be specifically and efficiently evaluated, and a new tool is provided for chemical safety evaluation and male infertility pathogenesis diagnosis.

The present invention discloses a method for assessing the activity of Takifugu sperm cryopreservation solution based on computer vision. [Solution] Takifugu sperm are cryopreserved for long periods using a cryopreservation solution, thawed, and then activated. After observation under a microscope, sperm motility videos are recorded. Data augmentation is performed on the video using a binarization method. Based on two deep learning algorithms for target detection and target tracking, the positional changes of multiple Takifugu sperm targets between consecutive frames are tracked and captured, and the significant movements and trajectories of the recognized sperm are analyzed for imitation training. The sperm's movement and viability are determined by statistically analyzing their displacement between different frames. Finally, a visualized sperm viability graph is generated. This method enables long-term cryopreservation of highly active Takifugu sperm, providing technical support for year-round reproduction and species resource conservation. It also improves the accuracy of Takifugu sperm activity detection, simplifies the detection and recognition process, and reduces costs.

The invention relates to the use of compounds of formula (I), stereoisomers, and physiologicaily tolerable salts thereof, to enhance the motility of sperm obtained from a non-human mammal: wherein in formula I: X is —NR′— (in which R′ is H or C1-3 alkyl. e.g. —CH3), —O—, —S— or —CH2—; Y is —S—, —O—, —NR″— (in which R″ is H or C1-3 alkyl, e.g. —CH3), or —CH2—; Z1 and Z2 are independently selected from N and CH; R1 to R3 are independently selected from hydrogen, halogen, C1-6 alkyl, C1-6 alkoxy, C1-6 haloalkyl, —OH, —CN, —NO2 and —NR12R13; R4 is hydrogen, halogen, C1-6 alkyl, C1-6 alkoxy, C1-6 haloalkyl, or —OH; R5 is hydrogen, halogen, C1-6 alkyl, C1-6 alkoxy, or C1-6 haloalkyl; R6 and R7 are independently selected from hydrogen, halogen, C1-6 alkyl, C1-6 alkoxy, C1-6 haloalkyl, —OH, —CN, —NO2 and —NR12R13; R8 is hydrogen, halogen, C1-6 alkyl, C1-6 alkoxy, C1-6 haloalkyl, —CN, —NO2 or —NR12R13; R9 is hydrogen or C1-4 alkyl; R10 and R11 are independently selected from hydrogen and C1-3 alkyl; or R10 and R11, together with the intervening —O— and ring atoms, are joined to form a dioxolane ring; and R12 and R13 are independently selected from H and C1-3 alkyl. The compounds find particular use in animal husbandry to increase the rate of fertilization in artificial insemination of livestock, especially pigs. They can also be used to prepare, pre-treat or store semen for use in artificial insemination procedures.

The application belongs to the technical field of animal genetic engineering, and provides a DHX36 gene molecular marker related to the sperm vigor trait of a cow and application thereof, wherein the DHX36 gene molecular marker is a C-G base mutation at the 231st site of a bovine DHX36 gene fragment shown in the sequence table SEQ ID NO:1. Based on the DHX36 gene molecular marker, the genotype is determined by sequence determination, and the genotype is associated with the normal and post-thawing sperm vigor trait of the cow. The analysis result shows that the normal and post-thawing sperm vigor of individuals with different genotypes is significantly different. The application uses specific primers to obtain a bovine DHX36 gene fragment related to the normal and post-thawing sperm vigor trait of the cow, and uses a specific SNP site in the fragment as a molecular marker, thereby providing a theoretical basis and specific application for marker-assisted selection of the cow.

The invention is applicable to the technical field of animal genetic engineering, and provides an ATP5PO gene molecular marker related to bovine sperm motility traits and application of the ATP5PO gene molecular marker, the ATP5PO gene molecular marker is the 203th site of a bovine ATP5PO gene segment, the site has an A-G base mutation, resulting in generation of gene single nucleotide polymorphism; the nucleotide sequence of the bovine ATP5PO gene segment is as shown in a sequence table SEQ ID NO: 1. Based on the ATP5PO gene molecular marker, the genotype is judged through sequence determination, and the genotype can be associated with normal and unfrozen activity traits of bovine sperms. According to the invention, the specific primer group is utilized to obtain the bovine ATP5PO gene segment related to the normal and unfrozen viability traits of bovine sperms, and the specific SNP site in the segment is taken as the molecular marker, so that a theoretical basis and specific application are provided for marker-assisted selection of cattle.

The invention discloses application of sperm motility body fluid response characteristics in disease risk prediction. In the application, through quantitative analysis of motion parameters of mouse sperms after co-incubation with human serum from different sources, early prediction and diagnosis of high-risk diseases such as cerebral infarction (CI), myocardial infarction (MI) and pancreatitis can be realized, and the mouse sperm detection kit has the advantages of high accuracy, good sensitivity, low cost and the like. In specific implementation, after mouse sperms and patient serum are co-incubated, if the active sperm rate is lower than 14.3%, 10.9% or 20.6%, the active sperm rate can be respectively used as diagnosis thresholds of cerebral infarction, myocardial infarction or pancreatitis. Based on the technology, related products for high-risk disease diagnosis, such as diagnostic kits and diagnostic and screening equipment, can be further developed.