9 results about "Oligospermia" patented technology

The invention discloses a method for generating haploid sperm-like cells through in-vitro differentiation of pluripotent stem cells and application of the haploid sperm-like cells. Human pluripotent stem cells are firstly differentiated into ectoderm-like cells, then the ectoderm-like cells are spontaneously gathered and converted into primordial germ cell-like cells under the induction of ectoderm induction factors, and then the primordial germ cell-like cells and testicular sertoli cells are subjected to mixed culture to form recombinant testicular tissues; the recombinant testis tissue enters meiosis under the induction of nutrition limitation and retinoic acid, then meiosis is completed under the stimulation of meiosis second-stage induction factors such as testosterone, follicle-stimulating hormone and pituitary extract, and interaction between primordial germ cell-like cells and testis supporting cells is enhanced by using small molecules SB431542, so that the meiosis of the testis is enhanced. And finally inducing to generate haploid sperm-like cells. According to the invention, a new tool can be provided for researching a molecular mechanism of male reproduction, and an important thought can be provided for research and transformation of asthenospermia, oligospermia, drug screening and the like.

The invention provides a traditional Chinese medicine composition for tonifying kidney, strengthening yang and tonifying deficiency for treating male erectile dysfunction and oligospermia and asthenospermia. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 220 to 500 parts of semen cuscutae, 350 to 650 parts of bull penis, 230 to 480 parts of herba epimedii, 210 to 470 parts of herba cistanche, 190 to 450 parts of fructus corni, 180 to 420 parts of tortoise shell, 170 to 400 parts of antler, 160 to 350 parts of hippocampus, 160 to 280 parts of ginseng, 170 to 300 parts of rhizoma polygoni cuspidati, 210 to 380 parts of cortex eucommiae, 160 to 310 parts of okra seed, 130 to 250 parts of pericarpium citri reticulatae, 210 to 450 parts of caulis spatholobi, 250 to 360 parts of caulis sargentodoxae and 180 to 380 parts of rhizoma chuanxiong. The traditional Chinese medicine composition is used for treating male erectile dysfunction and insufficiency of kidney yang, impotence, spermatorrhea, premature ejaculation and sexual dysfunction in oligospermia and asthenospermia, can be used for symptomatically improving the weakness of male middle-aged and elderly people, has a long-term effect and stability after treatment, is superior to other treatment medicines and treatment technologies in modern medicine, and has a wide application prospect. Wide clinical value and commercial value are realized.

The invention discloses a nutrient composition for improving the male fertility function and a preparation method and application of the nutrient composition. The composition is composed of zinc, selenium, coenzyme Q10, L-acetyl carnitine, folic acid, vitamin D3, natural lycopene, vitamin E, L-arginine and core components such as standardized herba epimedii, fructus lycii and maca extract according to a specific ratio, and three pairs of key mass ratios of zinc / selenium, L-acetyl carnitine / coenzyme Q10 and natural lycopene / vitamin E are limited for the first time. The composition can synergistically improve sperm quality, reduce oxidative stress and improve serum testosterone level in a multi-target manner, the invention further provides a preparation method of the composition, the uniformity and stability of the composition are ensured through the processes of graded mixing, low-temperature sterilization and the like, and the composition can be used for preparing dietary supplements or health care products for improving oligospermia, asthenozoospermia and abnormal spermatozoospermia.

The present application relates to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition and application thereof in preparation of a medicine for improving and / or treating male infertility. The traditional Chinese medicine composition is prepared from Fructus Malli, Fructus Equiseti, Rhiocarpae, Radix Rehmanniae Preparatae, Rubus chingii Hu, Fructus Carpesii, Rhizoma Polygonati, Morus, Fructus Lycii, Semen Marsdeniae, Fructus Schisandrae Chinensis, Semen Plantaginis, Fructus Rosae Laevigatae, Radix Glycyrrhizae Preparatae. The traditional Chinese medicine composition is supplemented with multiple medicinal materials on the basis of a traditional prescription, forms a comprehensive conditioning scheme considering multiple viscera and multiple causes, a multi-dimensional syndrome differentiation and treatment strategy, and is beneficial to the treatment purpose of treating both the symptoms and the root cause, can more effectively cope with the multiple pathological changes of oligospermia and asthenospermia of male infertility, and improve the overall treatment effect. The traditional Chinese medicine composition is applied in cooperation with an assisted reproductive technology, and the effect is remarkable.

This invention discloses an in vivo sperm processing device, control method, and application based on surface acoustic wave (SAW) devices. The non-invasive in vivo sperm processing method based on SAW devices offers significant advantages: it avoids the trauma risks of surgical and acupuncture treatments and solves the problem of unstable effects from hormone therapy and antioxidant therapy. By precisely controlling SAW parameters, this invention significantly improves sperm motility. It is simple to operate, low in cost, and highly safe, and applicable to various types of oligospermia and asthenospermia, providing a highly efficient, non-invasive, and widely applicable new solution for the research and treatment of oligospermia and asthenospermia.

The invention belongs to the technical field of traditional Chinese medicine compositions, and discloses a composition for promoting sperm quality improvement and resisting aging and a preparation method and application thereof, the composition is composed of sun-dried ginseng, fructus alpiniae oxyphyllae, pericarpium citri reticulatae, nutmeg, gardenia, raspberry, dark plum, pawpaw and deer blood freeze-dried powder; the dosage forms of the composition comprise decoction, powder, superfine powder, pills and the like. The composition is applied to preparation of a medicine for treating and / or relieving oligospermia and asthenozoospermia; the traditional Chinese medicine composition disclosed by the invention runs through ancient and modern prescription medicines, the preparation process is mature and controllable, and a novel traditional Chinese medicine choice which is safe, effective and clear in mechanism is provided for clinical treatment of asthenospermia and related anti-aging.

The application provides application of pyridoxamine in sperm of oligospermia, preferably and / or in a freezing and resuscitation operation, pyridoxamine is added in a sperm operation liquid or a semen freezing liquid, and the following shortcomings of patients with oligospermia are effectively improved: (1) the ratio of sperm forward motility; (2) the ratio of sperm forward motility after semen freezing and resuscitation; (3) the sperm DNA fragmentation rate after semen freezing and resuscitation; (4) the sperm acrosome enzyme content after semen freezing and resuscitation; in summary, pyridoxamine can be added in the sperm operation liquid or the semen freezing liquid as a natural semen protective agent, the sperm selection treatment and the freezing and resuscitation effect of patients with oligospermia are obviously improved, and the application is suitable for application of patients with oligospermia in assisted reproductive treatment and fertility preservation.

The invention discloses a hydroxysafflor yellow A modified mitochondria, which is prepared by the following method: mixing and stirring HSYA and mitochondria suspension in PBS (Phosphate Buffer Solution), centrifugally filtering, and then washing to remove uncombined HSYA. The invention also discloses application of the compound in preparation of medicines for treating asthenospermia. The invention provides a powerful, accurate and convertible strategy for resisting male infertility related to environmental toxicants.

The invention relates to the technical field of biological medicines, in particular to application of indirubin in preparation of a medicine for treating oligoasthenospermia. The indirubin disclosed by the invention is a natural product or an artificially synthesized product, is high in safety and small in toxic and side effects, and has a good effect of treating oligoasthenospermia.