20 results about "Oligospermia" patented technology

The invention discloses a method for generating haploid sperm-like cells through in-vitro differentiation of pluripotent stem cells and application of the haploid sperm-like cells. Human pluripotent stem cells are firstly differentiated into ectoderm-like cells, then the ectoderm-like cells are spontaneously gathered and converted into primordial germ cell-like cells under the induction of ectoderm induction factors, and then the primordial germ cell-like cells and testicular sertoli cells are subjected to mixed culture to form recombinant testicular tissues; the recombinant testis tissue enters meiosis under the induction of nutrition limitation and retinoic acid, then meiosis is completed under the stimulation of meiosis second-stage induction factors such as testosterone, follicle-stimulating hormone and pituitary extract, and interaction between primordial germ cell-like cells and testis supporting cells is enhanced by using small molecules SB431542, so that the meiosis of the testis is enhanced. And finally inducing to generate haploid sperm-like cells. According to the invention, a new tool can be provided for researching a molecular mechanism of male reproduction, and an important thought can be provided for research and transformation of asthenospermia, oligospermia, drug screening and the like.

The invention discloses application of exosome-like nanoparticles derived from fructus lycii to preparation of a medicine for preventing or treating oligospermia and / or asthenospermia. Sterile, stable and high-purity fructus lycii exosome-like nanoparticles (LBELNs) are obtained by adopting a differential centrifugation-suction filtration-ultra-high-speed centrifugation method, the physiological efficacy of the LBELNs is researched, and it is found that the LBELNs can significantly improve the oligoasthenospermia disease activity index of male mice induced by cyclophosphamide (CTX); the sperm quantity is obviously increased, the sperm malformation is improved, the sperm motility is improved, and the sex hormone level is recovered. The LBELNs can effectively relieve spermatogenic dysfunction of testicular tissues, the sperm differentiation and formation process is obviously improved, the total number of sperms is increased, and the treatment effect is more obvious along with increase of the dosage of the LBELNs. The invention has potential in preparation of drugs for preventing or treating oligoasthenospermia, and provides a new strategy and treatment method for treating oligoasthenospermia.

The invention provides oleanolic acid, and a preparation method of the oleanolic acid comprises the following steps: S1, dissolving 5g of arginic acid in 500ml of dichloromethane, adding 7g of pyridinium dichlorochromate, stirring at room temperature for 18 hours, and detecting the reaction degree by thin-layer chromatography; s2, after the reaction is completed and vacuum concentration is carried out, a sample is mixed with 200-300-mesh silica gel, the mixture is volatilized to be dry and then placed on a silica gel column, column chromatography separation is carried out, petroleum ether / ethyl acetate is selected as a mobile phase, 2.87 g of oleanolic acid is obtained, the molecular formula is C30H46O3, and the yield is 57.6%. The oleanolic acid provided by the invention can be used for preparing medicines for treating oligospermia and medicines for improving dyszoospermia. The oleanolic acid provided by the invention is a natural product or an artificially synthesized product, is low in toxicity and small in side effect, and has good effects of improving dyszoospermia and promoting fertility.

The invention provides a traditional Chinese medicine composition for tonifying kidney, strengthening yang and tonifying deficiency for treating male erectile dysfunction and oligospermia and asthenospermia. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 220 to 500 parts of semen cuscutae, 350 to 650 parts of bull penis, 230 to 480 parts of herba epimedii, 210 to 470 parts of herba cistanche, 190 to 450 parts of fructus corni, 180 to 420 parts of tortoise shell, 170 to 400 parts of antler, 160 to 350 parts of hippocampus, 160 to 280 parts of ginseng, 170 to 300 parts of rhizoma polygoni cuspidati, 210 to 380 parts of cortex eucommiae, 160 to 310 parts of okra seed, 130 to 250 parts of pericarpium citri reticulatae, 210 to 450 parts of caulis spatholobi, 250 to 360 parts of caulis sargentodoxae and 180 to 380 parts of rhizoma chuanxiong. The traditional Chinese medicine composition is used for treating male erectile dysfunction and insufficiency of kidney yang, impotence, spermatorrhea, premature ejaculation and sexual dysfunction in oligospermia and asthenospermia, can be used for symptomatically improving the weakness of male middle-aged and elderly people, has a long-term effect and stability after treatment, is superior to other treatment medicines and treatment technologies in modern medicine, and has a wide application prospect. Wide clinical value and commercial value are realized.

This invention discloses a ginseng and deer antler pill and its preparation method, belonging to the field of traditional Chinese medicine preparation technology. The ginseng and deer antler pill comprises active ingredients and pharmaceutical excipients; wherein, the active ingredients, by mass parts, include: 9-11 parts ginseng, 15-17 parts deer antler, 11-13 parts tortoise shell, 9-11 parts donkey-hide gelatin, 15-17 parts motherwort, 15-17 parts morinda root, 15-17 parts angelica root, 15-17 parts eucommia bark, 15-17 parts achyranthes root, 15-17 parts poria cocos, 15-17 parts dodder seed, 15-17 parts astragalus root, 15-17 parts longan pulp, 15-17 parts schisandra fruit, 15-17 parts phellodendron bark, 15-17 parts cyperus rhizome, and 4-5 parts cordyceps. This invention enhances the blood-nourishing and sperm-generating effects of the original formula by adding tortoise shell and donkey-hide gelatin to the traditional ginseng and deer antler pill formula, and by replacing psoralea corylifolia with motherwort in the formula, resulting in a significant therapeutic effect on oligospermia.

The invention provides a traditional Chinese medicine composition with kidney tonifying and essence strengthening functions and a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicine. The traditional Chinese medicine composition with the kidney tonifying and essence strengthening functions is prepared from the following raw materials in parts by weight: 8 to 22 parts of antler slices, 5 to 15 parts of prepared radix aconiti lateralis preparata slices, 8 to 23 parts of herba epimedii, 5 to 15 parts of radix morindae officinalis, 5 to 15 parts of herba cistanche, 8 to 22 parts of radix rehmanniae preparata, 8 to 22 parts of tribulus terrestris, 5 to 15 parts of turtle shells, 8 to 22 parts of tortoise shells, 5 to 15 parts of radix angelicae sinensis, 8 to 22 parts of fructus lycii, 3 to 9 parts of flos caryophylli, 15 to 45 parts of radix astragali seu hedysari, 5 to 15 parts of rhizoma polygonati and 3 to 7 parts of sun-dried ginseng. The traditional Chinese medicine composition is decocted with water to obtain a decoction, after the traditional Chinese medicine composition is administrated to a patient with oligospermia or asthenospermia, the semen quality of the patient can be effectively improved, the sperm density is improved, the sperm motility rate and the normal sperm form proportion are increased, and the problem of male infertility is solved.

The invention discloses a nutrient composition for improving the male fertility function and a preparation method and application of the nutrient composition. The composition is composed of zinc, selenium, coenzyme Q10, L-acetyl carnitine, folic acid, vitamin D3, natural lycopene, vitamin E, L-arginine and core components such as standardized herba epimedii, fructus lycii and maca extract according to a specific ratio, and three pairs of key mass ratios of zinc / selenium, L-acetyl carnitine / coenzyme Q10 and natural lycopene / vitamin E are limited for the first time. The composition can synergistically improve sperm quality, reduce oxidative stress and improve serum testosterone level in a multi-target manner, the invention further provides a preparation method of the composition, the uniformity and stability of the composition are ensured through the processes of graded mixing, low-temperature sterilization and the like, and the composition can be used for preparing dietary supplements or health care products for improving oligospermia, asthenozoospermia and abnormal spermatozoospermia.

The invention relates to the technical field of traditional Chinese medicines, in particular to a composition with the effects of tonifying kidney, strengthening essence and resisting oxidation as well as a preparation method and application thereof. According to the composition disclosed by the invention, the zinc-selenium composition, the compound yeast extract, the L-carnitine and the specific traditional Chinese medicine extract are combined in pairs, or three or four of the zinc-selenium composition, the compound yeast extract, the L-carnitine and the specific traditional Chinese medicine extract. Experiments show that the composition can generate a synergistic effect under a specific ratio, and the synergistic effect of four combinations is more obvious. The components of the composition are medicinal and edible components, and the composition has the effects of multiple antioxidation, kidney tonifying, essence strengthening and sperm motility improvement, is good in taste and high in compliance, and has a better effect on improving the symptoms of asthenospermia, insufficient sperm quantity, male function decline and sex hormone reduction.

The invention relates to an in-vitro culture solution for enhancing the motility of human sperms and application of the in-vitro culture solution. The culture solution contains 3-phosphoglyceric acid (3-PG). The culture solution can be used for culturing purified asthenospermia sperms under an in-vitro condition, and the forward movement capability and the total activity of the sperms are remarkably improved. The 3-PG in the culture solution is directly diluted and cultured by adopting the sperm culture solution, so that the possible toxic influence of organic solvents such as ethanol or DMSO (Dimethylsulfoxide) on sperms is avoided. Experimental results show that the sperm motility of clinical asthenospermia patients can be obviously enhanced by adding the 3-PG into the culture solution, the enhancement effect on moderate and severe asthenospermia is particularly obvious, and the sperm motility of most mild asthenospermia patients is recovered to a normal range. It is fully proved that 3-PG has the effect of effectively improving the sperm motility function of a patient with asthenospermia in in-vitro culture.

The invention discloses a new application of lenalidomide (CC-5013) in the treatment of male oligospermia and azoospermia caused by hepatitis E virus (HEV) infection or the improvement of sperm quality, and particularly discloses a new application of lenalidomide (CC-5013) in the treatment of male oligospermia and azoospermia caused by hepatitis E virus (HEV) infection. The lenalidomide is proved to have an obvious treatment effect on sperm quality reduction caused by HEV infection through sperm staining, sperm motility detection, testis pathology analysis, quantitative detection of sperm development key genes and other methods by utilizing an HEV infected mouse model. The treatment scheme provided by the invention is safe and effective, good in treatment effect and low in medicine cost, is suitable for rapid treatment of male reproductive injuries such as male azoospermia, oligospermia and poor sperm quality caused by virus infection, and provides medicine support for diagnosis and treatment of male infertility patients caused by HEV infection.

The present application relates to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition and application thereof in preparation of a medicine for improving and / or treating male infertility. The traditional Chinese medicine composition is prepared from Fructus Malli, Fructus Equiseti, Rhiocarpae, Radix Rehmanniae Preparatae, Rubus chingii Hu, Fructus Carpesii, Rhizoma Polygonati, Morus, Fructus Lycii, Semen Marsdeniae, Fructus Schisandrae Chinensis, Semen Plantaginis, Fructus Rosae Laevigatae, Radix Glycyrrhizae Preparatae. The traditional Chinese medicine composition is supplemented with multiple medicinal materials on the basis of a traditional prescription, forms a comprehensive conditioning scheme considering multiple viscera and multiple causes, a multi-dimensional syndrome differentiation and treatment strategy, and is beneficial to the treatment purpose of treating both the symptoms and the root cause, can more effectively cope with the multiple pathological changes of oligospermia and asthenospermia of male infertility, and improve the overall treatment effect. The traditional Chinese medicine composition is applied in cooperation with an assisted reproductive technology, and the effect is remarkable.

This invention discloses an in vivo sperm processing device, control method, and application based on surface acoustic wave (SAW) devices. The non-invasive in vivo sperm processing method based on SAW devices offers significant advantages: it avoids the trauma risks of surgical and acupuncture treatments and solves the problem of unstable effects from hormone therapy and antioxidant therapy. By precisely controlling SAW parameters, this invention significantly improves sperm motility. It is simple to operate, low in cost, and highly safe, and applicable to various types of oligospermia and asthenospermia, providing a highly efficient, non-invasive, and widely applicable new solution for the research and treatment of oligospermia and asthenospermia.

The invention belongs to the technical field of traditional Chinese medicine compositions, and discloses a composition for promoting sperm quality improvement and resisting aging and a preparation method and application thereof, the composition is composed of sun-dried ginseng, fructus alpiniae oxyphyllae, pericarpium citri reticulatae, nutmeg, gardenia, raspberry, dark plum, pawpaw and deer blood freeze-dried powder; the dosage forms of the composition comprise decoction, powder, superfine powder, pills and the like. The composition is applied to preparation of a medicine for treating and / or relieving oligospermia and asthenozoospermia; the traditional Chinese medicine composition disclosed by the invention runs through ancient and modern prescription medicines, the preparation process is mature and controllable, and a novel traditional Chinese medicine choice which is safe, effective and clear in mechanism is provided for clinical treatment of asthenospermia and related anti-aging.

The invention belongs to the technical field of compositions, and provides a medicinal and edible composition for conditioning male oligospermia and asthenospermia and a preparation method of the medicinal and edible composition. The medicinal and edible composition is prepared from the following raw materials: oyster peptide powder, fructus lycii, rhizoma polygonati, radix ginseng, maca, concentrated pomegranate juice and concentrated mulberry juice. The preparation method comprises the following steps: (1) weighing rhizoma polygonati, ginseng, fructus lycii and maca, mixing, crushing and sieving to obtain mixed powder; (2) extracting the mixed powder to obtain a mixed filtrate; concentrating to obtain a second concentrated solution, sterilizing and storing; and (3) dissolving the oyster peptide powder in water, adding the concentrated pomegranate juice, the concentrated mulberry juice and the second concentrated solution, mixing, and filling into a container. The composition with homology of medicine and food has the advantages that the used raw materials are few and fine, the compatibility effect is strong, and the composition has a remarkable spermatogenic function on mice with deficiency of kidney-yang and has an application prospect of improving the symptoms of oligospermia and asthenozoospermia of men; the flavor is good. The preparation method is good in effective component extraction effect and simple to operate, and industrial large-scale production can be realized.

The application provides application of pyridoxamine in sperm of oligospermia, preferably and / or in a freezing and resuscitation operation, pyridoxamine is added in a sperm operation liquid or a semen freezing liquid, and the following shortcomings of patients with oligospermia are effectively improved: (1) the ratio of sperm forward motility; (2) the ratio of sperm forward motility after semen freezing and resuscitation; (3) the sperm DNA fragmentation rate after semen freezing and resuscitation; (4) the sperm acrosome enzyme content after semen freezing and resuscitation; in summary, pyridoxamine can be added in the sperm operation liquid or the semen freezing liquid as a natural semen protective agent, the sperm selection treatment and the freezing and resuscitation effect of patients with oligospermia are obviously improved, and the application is suitable for application of patients with oligospermia in assisted reproductive treatment and fertility preservation.

The invention discloses a method and a system for evaluating male fertility by analyzing meiosis recombination rate. The method comprises the following steps: acquiring a diploid biological sample and a plurality of monomer sperm cells of a subject; respectively determining the diploid and haploid genotypes on the heterozygous loci through sequencing; identifying and filtering meiosis crossover events for each sperm cell based on the genotype data, thereby calculating a quantitative crossover rate; the crossover rate is finally compared to a reference crossover rate from a normal fertility population to assess the fertility of the subject. The key point of the invention lies in that it is revealed for the first time that reduction of meiosis crossover rate is a potential quantitative biomarker of'uniform cell energy deficiency 'in male germ cell lines, and the method can more deeply, objectively and accurately evaluate the internal health status of sperms by measuring the core development event. The kit is especially suitable for diagnosis and risk assessment of asthenospermia and monitoring of infertility treatment effect.

The invention discloses a hydroxysafflor yellow A modified mitochondria, which is prepared by the following method: mixing and stirring HSYA and mitochondria suspension in PBS (Phosphate Buffer Solution), centrifugally filtering, and then washing to remove uncombined HSYA. The invention also discloses application of the compound in preparation of medicines for treating asthenospermia. The invention provides a powerful, accurate and convertible strategy for resisting male infertility related to environmental toxicants.

The invention provides a kit or a chip (4 bile acids) for evaluating a spermatogenic function of a testis. The kit or the chip comprises detection reagents capable of detecting the concentrations of the following four bile acids: NorCA, GUDCA, T-alpha-MCA and GDCA. The invention further provides a kit or chip (10 bile acid) for evaluating the spermatogenic function of the testis. The kit or chip comprises detection reagents capable of detecting the concentration of the following ten bile acids: THCA, NorCA, TDCA, GHCA, GLCA, GUDCA, GCDCA, GCA, T-alpha-MCA and GDCA. By detecting the concentration of a series of bile acids in the plasma of a patient, the evaluation of the spermatogenic function and other testis spermatogenic states of male infertility is reflected. The method is suitable for evaluating various testicular spermatogenesis disorder states such as non-obstructive azoospermia, oligospermia, asthenospermia and spermatogonia, has the advantages of no need of sperm collection, high noninvasiveness, high sensitivity and wide applicable population, and is suitable for evaluating the male fertility rate and assisting in formulating an individualized assisted reproduction diagnosis and treatment scheme.

The invention relates to the technical field of biological medicines, in particular to application of indirubin in preparation of a medicine for treating oligoasthenospermia. The indirubin disclosed by the invention is a natural product or an artificially synthesized product, is high in safety and small in toxic and side effects, and has a good effect of treating oligoasthenospermia.

The invention relates to a traditional Chinese medicine composition for male oligospermia as well as a preparation method and application thereof, and relates to the technical field of traditional Chinese medicines. The traditional Chinese medicine composition comprises ginseng, cornu cervi pantotrichum, codonopsis pilosula, angelica sinensis, prepared rehmannia root, astragalus membranaceus, radix paeoniae alba, ligusticum wallichii, corydalis tuber, fenugreek, teasel root, bighead atractylodes rhizome, rhizoma cyperi, fructus amomi, leonurus, scutellaria baicalensis, parasitic loranthus and honey-fried licorice root. The traditional Chinese medicine composition can treat male oligospermia well by improving testis injury, raising sperm motility and adjusting sex hormone balance.