5 results about "Phosphoglyceric acid" patented technology

The invention discloses a polyamine carbon dot as well as a preparation method and application thereof in improvement of corn resistance, and belongs to the technical field of nano agricultural regulation and control. The polyamine carbon dots are subjected to hydrothermal synthesis, the size of the PEICDs is 2.3 nm, the quantum yield of the carbon dots can be increased, carbon dioxide in the atmosphere can be captured and converted into bicarbonate radicals, and it is verified through in-vitro experiments that the PEICDs can improve the carbon dioxide conversion product 3-phosphoglycerate in vitro; under the stress of drought and salt, the polyethyleneimine functionalized carbon dot solution is applied to the leaf surfaces of the crops, so that the growth and development of the crops can be improved by remarkably improving the net photosynthetic rate and the intercellular carbon dioxide concentration, and the adaptability of the crops to the environment is further improved.

The invention relates to the technical field of genetic engineering, in particular to a recombinant bacterium for high-yield serine and application of the recombinant bacterium in fermentation production of serine. The ptsI gene of escherichia coli is knocked out, a glucose PTS system is blocked, glucose transport and phosphorylation and PEP-pyruvic acid associated reaction are relieved, and pyruvic acid is synthesized by reducing serine synthesis precursor 3-phosphoglyceric acid branches. Growth adaptive evolution is further carried out on escherichia coli with the ptsI gene knocked out, an obtained mutant strain is sequenced, the mutation condition is shown in a table 2, and finally the growth rate accelerating chassis bacterium is obtained. Afterwards, by enhancing the expression serine key genes serA, serB and serC of the mutant strain and knocking out serine degradation pathway genes sdaA, sdaB and tdcG, the engineering bacterium for fermentation production of serine is constructed, and the serine yield of the engineering bacterium is remarkably improved.

This invention provides a recombinant bacterium and its applications. After specific genetic engineering modifications, this recombinant bacterium can synthesize polyhydroxybutyrate (PHB) using one-carbon compounds such as methanol or carbon dioxide as a carbon source. Specifically, the metabolic pathway of the recombinant bacterium, after optimization, enables the efficient synthesis of PHB from methanol as a raw material via metabolic intermediates such as formaldehyde, 3-phosphoglyceric acid, and acetyl-CoA. The recombinant bacterium constructed in this invention can effectively utilize low-cost carbon sources such as methanol or carbon dioxide to produce PHB, and has promising prospects for industrial application.

This invention discloses a magnetic nanoprobe and kit for assessing postherpetic neuralgia. The magnetic nanoprobe is a complex formed by thiolized carbonized polymer dots, a chelating agent, superparamagnetic molecules, and 2-phospho-D-glyceric acid. 2-phospho-D-glyceric acid specifically reacts with NSE, dehydrating to generate phosphoenolpyruvate and releasing water molecules. The magnetic nanoprobe is specific for NSE and can be used for assessing postherpetic neuralgia. The kit includes the magnetic nanoprobe and instructions, and can determine the NSE marker content in a single sample, with a detection limit better than 1 pg / mL, thereby achieving effective assessment of postherpetic neuralgia.

The invention discloses a method for producing L-aromatic amino acid by improving the utilization efficiency of carbon atoms. In order to break through the carbon efficiency bottleneck in aromatic amino acid production, a rubisco gene (encoding 1, 5-diphosphate carboxylase / oxygenase) from rhodospirillum rubrum and a prk gene (encoding ribulose phosphate kinase) from spinach are integrated into pseudogene loci of ytfI and ygaQ of an escherichia coli genome, a Kalvin circulation path is constructed, and the carbon efficiency bottleneck in aromatic amino acid production is broken through. The method aims at recovering CO2 and regenerating 3-phosphoglyceric acid to enter central carbon metabolism, and promoter optimization is combined, so that the utilization efficiency of carbon atoms is further improved.