10 results about "Phosphoglyceric acid" patented technology

The invention belongs to the technical field of aquaculture, and particularly relates to a feed additive for aquaculture as well as a preparation method and application of the feed additive. The preparation method of the feed additive comprises the following steps: (1) weighing the raw material medicines, adding water for soaking, boiling with strong fire, then decocting with slow fire, and collecting first filtrate and first filter residues; (2) adding water into the first filter residue, boiling with strong fire, decocting with slow fire, collecting a second filtrate, and concentrating to obtain a traditional Chinese medicine composition extracting solution; (3) adding yeast powder, peptone, dipotassium phosphate, inositol galactoside and 1, 3-diphosphoglyceric acid into the traditional Chinese medicine composition extracting solution to obtain a fermentation culture medium; and (4) inoculating the lactobacillus fermentum liquid and the bacillus subtilis liquid into the fermentation culture medium for fermentation to obtain a fermentation mixture, and performing centrifugation and freeze drying to obtain the lactobacillus fermentum and bacillus subtilis compound feed. The feed additive for aquaculture is added into feed, so that the growth and development capability of animals can be effectively promoted, the immunocompetence of the animals is improved, and the feed additive has a wide application prospect.

The invention discloses a polyamine carbon dot as well as a preparation method and application thereof in improvement of corn resistance, and belongs to the technical field of nano agricultural regulation and control. The polyamine carbon dots are subjected to hydrothermal synthesis, the size of the PEICDs is 2.3 nm, the quantum yield of the carbon dots can be increased, carbon dioxide in the atmosphere can be captured and converted into bicarbonate radicals, and it is verified through in-vitro experiments that the PEICDs can improve the carbon dioxide conversion product 3-phosphoglycerate in vitro; under the stress of drought and salt, the polyethyleneimine functionalized carbon dot solution is applied to the leaf surfaces of the crops, so that the growth and development of the crops can be improved by remarkably improving the net photosynthetic rate and the intercellular carbon dioxide concentration, and the adaptability of the crops to the environment is further improved.

The invention relates to the technical field of genetic engineering, in particular to a recombinant bacterium for high-yield serine and application of the recombinant bacterium in fermentation production of serine. The ptsI gene of escherichia coli is knocked out, a glucose PTS system is blocked, glucose transport and phosphorylation and PEP-pyruvic acid associated reaction are relieved, and pyruvic acid is synthesized by reducing serine synthesis precursor 3-phosphoglyceric acid branches. Growth adaptive evolution is further carried out on escherichia coli with the ptsI gene knocked out, an obtained mutant strain is sequenced, the mutation condition is shown in a table 2, and finally the growth rate accelerating chassis bacterium is obtained. Afterwards, by enhancing the expression serine key genes serA, serB and serC of the mutant strain and knocking out serine degradation pathway genes sdaA, sdaB and tdcG, the engineering bacterium for fermentation production of serine is constructed, and the serine yield of the engineering bacterium is remarkably improved.

The invention relates to a berberine derivative as well as a preparation method and application thereof. The berberine derivative can be applied as a PGAM1 inhibitor. The compound shows good or excellent inhibition activity on phosphoglyceric acid mutant enzyme 1, wherein the enzymatic activity of a compound 18 (IC50 = 0.081 [mu] M), the enzymatic activity of a compound 22 (IC50 = 0.076 [mu] M) and the enzymatic activity of a compound 35 (IC50 = 0.087 [mu] M) are equivalent to the enzymatic activity of a positive drug (IC50 = 0.052 [mu] M). The biological activity of the compound is evaluated through in-vitro experiments, it is found that the compound has high anti-proliferative activity on non-small cell lung cancer cells and shows time dose dependence, and the tumor cell inhibition activity of the compound is obviously higher than that of a positive drug. A flow cytometry detects the influence on cell apoptosis, cycle, active oxygen and mitochondrial membrane potential, and finds that the compound can induce cell apoptosis and retard the cell cycle at a G2 / M period.

The invention relates to the technical field of L-cysteine production, and discloses a genetically engineered bacterium with high yield of L-cysteine as well as a construction method and application of the genetically engineered bacterium. Through knockout of a tcyP gene, overexpression of a PqqU gene, a galP gene, a zwf gene, a talA gene and a pta gene and introduction and overexpression of an fxpK gene, a carbon metabolic flux is reasonably distributed between an important carbon metabolic node 3-phosphoglycerate and acetyl coenzyme A necessary for an SAT catalytic reaction, the SAT can efficiently catalyze L-serine to synthesize OAS, and overflow of the L-serine is reduced. Finally, through the above strategy, more carbon metabolism flows to synthesis of L-cysteine in the strain fermentation process, and efficient production of L-cysteine is realized.

This invention provides a recombinant bacterium and its applications. After specific genetic engineering modifications, this recombinant bacterium can synthesize polyhydroxybutyrate (PHB) using one-carbon compounds such as methanol or carbon dioxide as a carbon source. Specifically, the metabolic pathway of the recombinant bacterium, after optimization, enables the efficient synthesis of PHB from methanol as a raw material via metabolic intermediates such as formaldehyde, 3-phosphoglyceric acid, and acetyl-CoA. The recombinant bacterium constructed in this invention can effectively utilize low-cost carbon sources such as methanol or carbon dioxide to produce PHB, and has promising prospects for industrial application.

This invention discloses a magnetic nanoprobe and kit for assessing postherpetic neuralgia. The magnetic nanoprobe is a complex formed by thiolized carbonized polymer dots, a chelating agent, superparamagnetic molecules, and 2-phospho-D-glyceric acid. 2-phospho-D-glyceric acid specifically reacts with NSE, dehydrating to generate phosphoenolpyruvate and releasing water molecules. The magnetic nanoprobe is specific for NSE and can be used for assessing postherpetic neuralgia. The kit includes the magnetic nanoprobe and instructions, and can determine the NSE marker content in a single sample, with a detection limit better than 1 pg / mL, thereby achieving effective assessment of postherpetic neuralgia.

The invention relates to the technical field of metabolome biologic detection, in particular to an erythrocyte metabolism marker for biological age prediction, a screening method, a prediction model and application of the erythrocyte metabolism marker. The erythrocyte metabolism marker comprises one or more of ornithine, hypoxanthine, ribose-1 / 5-phosphoric acid, ribose, glycine, glutathione, lactic acid, 6-glucose phosphate, 2, 3-diphosphoglyceric acid, dehydroepiandrosterone, glyceric acid, uric acid or inosine. The erythrocyte metabolism marker has the characteristics of strong correlation, simple collection and stable result, and the prediction precision is far higher than that of a conventional scheme; and the constructed KDM model can accurately and obviously predict the biological age change.

The application and preparation of metabolic biomarkers in the development of products for monitoring heart failure can be used for the early diagnosis of heart failure. These biomarkers are highly sensitive, rapid, convenient, and provide accurate and reliable results, offering a basis for clinical decision-making and laying a foundation for subsequent basic and clinical research. They have potential application and research value. The metabolic biomarkers include one or more of the following: 1-(4-nitrophenyl)piperidine, sphingolipid d19:3 / 23:0, phosphatidylethanolamine 16:1e / 22:5, phosphatidylethanolamine 16:1e / 22:6, sphingolipid d14:0 / 22:1, 3-phosphoglyceric acid, phosphatidylcholine 15:0 / 20:4, phosphatidylcholine o-16:1 / 18:0, phosphatidylcholine 14:0e / 20:1, phosphatidylcholine 17:0 / 18:5, betaine, phosphatidylcholine 15:0 / 18:2, and phosphatidylcholine 18:5e / 20:4.

The invention discloses a method for producing L-aromatic amino acid by improving the utilization efficiency of carbon atoms. In order to break through the carbon efficiency bottleneck in aromatic amino acid production, a rubisco gene (encoding 1, 5-diphosphate carboxylase / oxygenase) from rhodospirillum rubrum and a prk gene (encoding ribulose phosphate kinase) from spinach are integrated into pseudogene loci of ytfI and ygaQ of an escherichia coli genome, a Kalvin circulation path is constructed, and the carbon efficiency bottleneck in aromatic amino acid production is broken through. The method aims at recovering CO2 and regenerating 3-phosphoglyceric acid to enter central carbon metabolism, and promoter optimization is combined, so that the utilization efficiency of carbon atoms is further improved.