49 results about "Phosphoglyceric acid" patented technology

The invention discloses a method for raising hydrogen production efficiency of microalgae and belongs to the field of a biological energy source. The method comprises the following steps: hydrogen-production microalgae cells are cultured in a medium until the exponential growth phase; the microalgae cells are collected, and the microalgae cells are transferred to a microalgae hydrogen-production medium containing organic matters, deoxygenization and sealing are carried out, and the microalgae cells are continuously cultured in a dark environment for a certain time, wherein the organic matters contain at least one of glucose, fructose, acetate, pyruvic acid, 3-phosphoglycerate, malic acid and amino acid and at least one of sodium salts and sylvite of the above organic matters; and finally, the microalgae cells are again placed in a light environment to induce water decomposition to produce hydrogen. It is verified through experiments that hydrogen production efficiency of microalgae can be remarkably raised by adding the organic matters to the microalgae hydrogen-production medium. The method has characteristics of novel technology and remarkable effect and the like, is simple and reliable, and has important practical application value in the field of new energy.

This invention discloses a kind of genetic engineering bacteria with enhanced tryptophan anabolism, and the recombinant plasmid contained in the genetic engineering bacteria. The recombinant plasmid is prepared by: connecting tryptophan synthetase gene trpBA and phosphoglycerate dehydrogenase gene mutant M3serA gene in series to obtain M3BAT-I, a kind of genetic engineering bacteria with enhanced tryptophan anabolism, expressing induced by IPTG, and analyzing with SDS-PAGE to express tryptophan synthetase beta subunit, phosphoglycerate dehydrogenase M3 mutant, and tryptophan synthetaselpha subunit. After culture of the genetic engineering bacteria with enhanced tryptophan anabolism (tnaA gene removed), the tryptophan content in the supernatant is increased from 2 mg/L to 99 mg/L.

The invention discloses a preparation method of a composite paper wet strength agent and belongs to the technical field of preparation of the composite paper wet strength agent. According to the preparation method of the composite paper wet strength agent, oxalic acid is activated by carbonyldimidazole, 3-phosphoglyceric acid serves as a modifying agent, catalytic reaction is conducted by a palladium carbon catalyst, pre-modified polyamide mixed liquid is prepared, microorganisms are extracted from a waste mushroom culture medium, epoxy chloropropane is added, free chloridions generated in the reaction process are consumed, and catalytic reaction is conducted by enzyme generated by the microorganisms, so that the composite paper wet strength agent is prepared. The preparation method of the composite paper wet strength agent has the beneficial effects that the preparation steps are simple, the obtained wet strength agent has a good wet strength effect and paper has high strength after the wet strength agent is used; and the microorganisms are modified by a modified bacterial solution prepared by utilizing the waste mushroom culture medium and an amino acid solution, so residual organochlorine can be effectively removed, the organochlorine content of the obtained wet strength agent is less than 0.09% and the solid content of the wet strength agent is more than 50.6%.

The invention provides a nucleoside high-yielding strain as well as a construction method and application thereof. The invention also provides a method for producing nucleoside by fermentation. The method comprises the following steps of (1) enhancing a serine hydroxymethyltransferase gene glyA, as shown in SEQ ID NO:2, of bacillus subtilis; and/or (2) weakening a 2,3-diphosphoglyceric acid independent phosphoglyceric acid mutant enzyme gene pgm of a coded NCBI reference sequence WP_003228330.1 on a chromosome of the bacillus subtilis; and (3) applying the strain obtained in the step (1) and/or the step (2) to fermentation production of the nucleoside. The engineered strain bacillus subtilis provided by the invention is the nucleoside high-yielding strain, can effectively accumulate the nucleoside, improves the yield of the nucleoside, and lays a foundation for industrial production of the nucleoside.

A fusion chimeric protein is described herein that can assemble a functional carboxysome core, which is able to fix carbon by taking atmospheric carbon dioxide and converting it into useful caron-containing compounds such as 3-phosphoglycerate (3-PGA).

A microorganism strain suitable for fermentative production of amino acids of the phosphoglycerate family or derivatives thereof and producible from a starting strain, is based upon a starting strain having an increased activity of a yfiK-gene product or of a gene product of a yfiK homologue. The microorganism strain is a member of the family Enterobacteriaceae and is a member of the species E. coli. A plasmid has a Yfik-gene with a promoter and a genetic element for the deregulation of cysteine metabolism coding for a serine O-acetyl transferase and being subject tot a reduced feedback inhibition by L-cysteine.

The invention discloses a kit and a method for measuring the content of 1,3-diphosphoglycerate based on a micromethod. A reagent of the kit comprises an extracting solution, a first reagent and a second reagent, wherein the extracting solution is prepared from Tris-HCl, EGTA, mercaptoethanol and glycerinum in a mixed mode, the first reagent is prepared from MgSO4.7H2O, NADH, ATP and glyceraldehyde-3-phosphate dehydrogenase in a mixed mode, and the second reagent is prepared from Tris-HCl. The method comprises the principle that the glyceraldehyde-3-phosphate dehydrogenase can reversely catalyze the 1,3-diphosphoglycerate and the NADH to generate glyceraldehyde-3-phosphate, inorganic phosphorus and NAD, the glyceraldehyde-3-phosphate dehydrogenase and the NADH are added into a reaction system, and by measuring the reduction amount of the NADH at 340 nm, the content of the 1,3-diphosphoglycerate can be reflected. The kit and the method for measuring the content of the 1,3-diphosphoglycerate have the characteristics that the application range is wide, operation is easy and convenient, the detection time is short, detection sensitivity is high, the cost is low, repeatability is good, and the accuracy rate is high.

The invention discloses a circulating tumor cell metabolic typing marker and application thereof, and the circulating tumor cell metabolic typing marker includes phosphoglycerate kinase 1PGK1 and 6-phosphate glucose dehydrogenase G6PD. Functional typing of circulating tumor cells (CTCs) is performed from the perspective of metabolic activity, and change trends and biological behaviors of the CTCscan be better reflected. Validation results in prostate cancer specimens indicate that GM+CTCs subtype is more correlated with tumor metastasis compared with EMT subtype, and can be used as an auxiliary diagnostic marker for tumor metastasis. Therefore, CTCs glucose metabolism typing is an important supplement to CTCs counting and EMT phenotype typing, can provide useful information for clinical disease assessment, and has a good application prospect in tumor metastasis diagnosis and monitoring.

The invention discloses a preparation method of an automobile windshield cleaning agent. Sodium, tripolyphosphate, ethanol, potassium sorbate, ethyl cellulose, 3-phosphoglyceric acid, anionic surfactant 1, sodium tripolyphosphate, nonionic surfactant 1, isopropanol and nano titanium sol were mixed and Stir evenly, and then use an emulsifier to mix at high speed. The beneficial effects of the present invention are: the present invention can effectively remove the stains on the windshield, and it has the advantages of strong cleaning ability, short cleaning time, no corrosion to the base material, no stimulation to the human body, no trace left after use, etc., and It can form a protective film on the outer surface of the windshield to prevent dust accumulation and prevent water stains from remaining for a certain period of time.

The invention discloses a D-(-)-3-phosphoglycerate bonded silica gel grease adsorbent, and relates to the technical field of grease adsorbents. The D-(-)-3-phosphoglycerate bonded silica gel grease adsorbent is prepared from the following steps: (1) sodium silicate and sulfuric acid are taken as raw materials, and gelling, acidifying treatment, aging, washing, chambering and drying are performed on the raw materials to obtain silica gel; (2) the silica gel and 3-bromopropanol are reacted to obtain an intermediate I; (3) the intermediate I and D-(-)-3-phosphoglyceric acid are reacted to obtainD-(-)-3-phosphoglycerate chiral molecule bonded grease silica gel; (4) the D-(-)-3-phosphoglycerate chiral molecule bonded grease silica gel is washed, and then is vacuum dried at room temperature toobtain the finished product D-(-)-3-phosphoglycerate bonded silica gel grease adsorbent; and (5) after the finished product D-(-)-3-phosphoglycerate bonded silica gel grease adsorbent passes inspection, packaging and storage are performed on the finished product. The prepared modified grease silica gel adsorbent has high selective adsorption on phospholipid and saponin in grease and the adsorptionrate reaches above 98%.

The invention discloses a formula of a strong decontamination cleaning agent, which comprises, in parts by weight, 5-12 parts of linear alkylbenzene sulfonate, 6-12 parts of 7-polyquaternium salt, 5-8 parts of white vinegar, 6~10 parts of coconut oil fatty acid diethanolamide, 4~6 parts of ethanol, 10~12 parts of sodium lauroyl sarcosinate, 2~4 parts of trioleyl phosphate, 3~6 parts of 3‑phosphoglyceric acid, lemon 1-2 parts of essential oil, 5-9 parts of sodium tripolyphosphate, 2-3 parts of alkyl glycoside, 1-3 parts of activator and 1-2 parts of nano-titanium sol. The beneficial effects of the present invention are: the present invention can effectively remove stains such as edible oil, machine oil, and lubricating oil, and can also be used to remove double-sided adhesive tape and self-adhesive. It has the advantages of wide application range, strong decontamination ability, and high decontamination efficiency Advantages, and will not cause adverse reactions to the skin, suitable for popularization.

The invention discloses a material for promoting repairing of the defect and deficiency tissue of teeth and a preparation method. The material is gel and comprises 0.2-0.5 g of calcium chloride, 0.2-0.8 g of mono potassium phosphate, 0.04-0.1 g of polyacrylic acid, 0.01-0.05 g of dental enamel matrix protein, 0.1-1.0 g of beta-phosphoric acid sodium glycerinate and 1000 g of distilled water. The preparation method of the material is easy to implement and low in cost and requirement for equipment. The defect and deficiency parts of the teeth are filled with the material, which can promote repairing of the tooth tissue, main components of a repairing layer are hydroxyapatite crystals, the composition of the repairing layer is basically identical to that of dental enamel, and the interface between the filling material and natural teeth is eliminated; the tooth cavities with the depth of 1 millimeter are filled with the material, and after seven days, the highest thickness of the repairing layer can reach 0.63 millimeter.

The invention discloses a preparation method of an automobile cleaning agent, comprising preparing C8-10 fatty alcohol polyoxyethylene ether phosphoric acid monoester, diethylenetriaminepentaacetate iron-sodium complex, sodium dodecylbenzenesulfonate, three Polyphosphate, ethanol, potassium sorbate, ethyl cellulose, 3-phosphoglyceric acid, anionic surfactant 1, sodium tripolyphosphate, nonionic surfactant 1, isopropanol and nano-titanium sol are mixed and stirred evenly, Then use an emulsifier for high-speed shear mixing. The beneficial effects of the present invention are: the present invention can effectively remove the stains on the outer surface of the automobile, and it has the advantages of strong cleaning ability, short cleaning time, no corrosion to the substrate, no stimulation to the human body, no trace left after use, etc., and can Form a protective film on the outer surface of the car to prevent dust from accumulating for a certain period of time.

The invention discloses a preparation method of a strong decontamination cleaning agent, comprising linear sodium alkylbenzene sulfonate, 7-polyquaternium salt, white vinegar, coconut oil fatty acid diethanolamide, ethanol, sodium lauroyl sarcosinate , trioleyl phosphate, 3-phosphoglyceric acid, lemon essential oil, sodium tripolyphosphate, alkyl glycoside, activator and nano titanium sol are mixed and stirred evenly, and then mixed by high-speed shearing with an emulsifier. The beneficial effects of the present invention are: the present invention can effectively remove stains such as edible oil, engine oil, lubricating oil, etc., and can also be used to remove double-sided adhesive tape and self-adhesive. Advantages, and will not cause adverse reactions to the skin, suitable for popularization.

The invention discloses a method for improving the tolerance of corynebacterium glutamicum to an inhibitor, which is characterized in that the activity of corynebacterium glutamicum is enhanced by reducing or eliminating the catalytic activity of endogenous formyltetrahydrofolate deformylase of the corynebacterium glutamicum or mutating endogenous D-3-phosphoglycerate dehydrogenase of the corynebacterium glutamicum, or the tolerance of the corynebacterium glutamicum to the inhibitor is improved by combining the two methods. Therefore, the recombinant corynebacterium glutamicum with improved tolerance to inhibitors is obtained. The strain constructed by the invention provides reference for construction of subsequent high-performance industrial fermentation chassis strains, and is beneficial to production of biofuels or other high-added-value chemicals by corynebacterium glutamicum by taking lignocellulose hydrolysate as a raw material.