84 results about "Rhodospirillum" patented technology

The invention discloses a biological purification agent applied to sewage of livestock and poultry farms and a preparation method thereof. The preparation method comprises the following steps: firstlyadding a compound microorganism bacterium agent into an immobilized carrier formed by three carriers including sodium alginate zeolite, sodium alginate and polyvinyl alcohol to obtain a bacterium solution, wherein the mass ratio of the immobilized carrier to the compound microorganism bacterium agent is (200-300) to 1, the compound microorganism bacterium agent contains rhodospirillum, nitrifyingbacteria and bacillus subtilis according to a volume ratio of 1 to 1 to 1, and the number of effective living bacteria in per gram of the compound microorganism bacterium agent is greater than or equal to 109; then dropping the bacterium solution into a boric acid saturated solution with 4-8% of CaCl2 to obtain globular immobilized compound microorganisms; finally enabling the immobilized compound microorganisms to stand and cure under the condition of 4 DEG C, carrying out immobilized crosslinking for 24 hours to obtain immobilized microorganism globules, washing the immobilized microorganism globules with deionized water for at least three times to obtain the biological purification agent. The preparation method is simple and is low in cost; the prepared purification agent has strong stability, and high microorganism activity, is environmentally friendly, and is free of secondary pollution.

The invention discloses a microbiological preparation for efficiently treating municipal lake water pollution. The microbiological preparation is prepared from nitrosospira, rhodospirillum, bacillus subtilis natto, kitasatospora phosalacinea and lactobcillus in a volume ratio of 0-10 to 5-15 to 5-15 to 5-15 to 5-15. The microorganism selected and cultured by the invention has the capability of being rapidly multiplied and developed into a dominant microflora under the municipal lake water environment; and each strain can independently and cooperatively decompose eutrophic ingredients in the municipal lake water and can make certain eutrophic ingredients which are difficult to decompose in a normal state easy to decompose. The microbiological preparation has the advantages of purifying the water quality well, improving the substrate, optimizing the biological environment, saving the biological environment from damage, having obvious effect on water raising, water retention, water color stabilization, treatment of turbid water, water concentrate, red water, black water, bottom odourization and algae aging and regulation and stabilization of pH value of the water body and making the lake water clear and odorless rapidly, along with simple using method, low cost, obvious effect, no secondary pollution.

The invention belongs to the technical field of wastewater treatment and particularly relates to a microbial agent suitable for treating urban wastewater and a preparation method thereof. The microbial agent comprises Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, saccharomycetes, lactic acid bacteria, acetic acid bacteria, photosynthetic bacteria, Actinobacteria, indigenous bacteria, nitrifying bacteria, denitrifying bacteria, phosphorus-accumulating bacteria, Rhodospirillum, Thiobacillus denitrificans, Sphingobacteria, Alcaligenes faecalis, Bifidobacterium, brown rot fungi,Geotrichum candidum, Mucor, Aspergillus oryzae, and Penicillium diversum. The preparation method includes: mixing well Actinobacteria, indigenous bacteria, nitrifying bacteria, denitrifying bacteria,phosphorus-accumulating bacteria and Bifidobacterium, adding the mixture into clean soil, farming earthworms with the soil for 12-14 days, and isolating the earthworms and the soil to obtain earthwormcast containing the mixed bacteria; mixing the earthworm cast containing the mixed bacteria and other bacteria, and immobilizing to obtain the microbial agent.

The invention discloses a compound microbiological preparation. The compound microbiological preparation comprises microorganisms and bio-enzyme, wherein the microorganisms contain bacillus subtilis,bacillus licheniformis, nitrosomonas, nitrosospira brieusis, P.Aeruginosa, paracoccus denitrificans, lactobacillus plantarum, lactobacillus casei, candida utilis, saccharomyces cerevisiae, rhodopseudomonas palustris, rhodospirillum, aspergillus niger and enterococcus faecalis; and the bio-enzyme contains microbial enzyme and an aerobic microbial agent. The compound microbiological preparation disclosed by the invention does not contain chemical substances, and secondary pollution to an environment is avoided, and therefore, the compound microbiological preparation is an environmental-friendlymicrobial agent. The preparation process of the compound microbiological preparation has the advantages that the preparation process is simple, the production cost is low, and the compound microbiological preparation has wide prospects on restoration of eutrophicated water bodies.

The invention provides a composite microbial disease/insect-resistant agent for green vegetables and a manufacturing method thereof. The composite microbial disease/insect-resistant agent for green vegetables comprises a composite microbial inoculant and a fertilizer, wherein the composite microbial inoculant is a mixed microbial inoculant formed by fermenting Bacillus and Rhodospirillum in a culture medium; the fertilizer comprises a potassium fertilizer and a pH regulator; the mass ratio of the composite microbial inoculant to the fertilizer is (30-50):(50-70); and the mass ratio of the Rhodospirillum microbial inoculant to the Bacillus microbial inoculant is (1-3):1. By adopting the composite microbial inoculant, the agent has the advantages of sufficient nutrients and reasonable supply, can enhance the nutrient absorption capacity of the plant root, and effectively inhibits harmful bacteria from growth and reproduction. When being used for a long time, the agent can improve the soil and avoid multiple diseases and insects due to deficiency of nutrient elements.

The invention relates to a resourceful treatment method for waste bodies of dead livestock and poultry animals. The resourceful treatment method comprises the following steps: refrigerating, storing and cutting dead animals, and crushing the refrigerated dead animals to obtain animal fragments; cooking the animal fragments; mixing the oil-removed animal fragments with a compound microbial agent to carry out micro-biological degradation; and carrying out medium-temperature aerobic fermentation on a degraded product to obtain biogas, biogas slurry and biogas residues, wherein the compound microbial agent consists of anabaena, bacillus subtilis, lactobacillus acidophilus, bacillus megatherium, bacillus cereus, bacillus pumilus, slender rhodospirillum, stenotrophomonas maltophilia and bacillus mojavensis. The resourceful treatment method solves the problem of treating dead bodies of the livestock and poultry animals of a large-scale breeding plant, so that resources are reused.

The invention relates to a preparation process of a flocculant for treating dyeing wastewater and an application method of the flocculant. The preparation process comprises the following steps: I) preparing an EM bacterial solution, II) cultivating bacterial colonies; and III) uniformly mixing 20 parts of the EM bacterial solution prepared in the step I with 1 part of a diluent of a mixed strain of Nocardia corallina, Rhodospirillum and nitrobacterium prepared in the step II. According to the preparation process disclosed by the invention, the EM bacterial solution is adopted as a main body, the combination of the characteristic role of microorganism strains and the capabilities of purifying water and degrading organic pollutants, nitrogen and ammonia in water bodies of Nocardia corallina, Rhodospirillum and nitrobacterium based on the EM bacteria technology and the EM bacterial genus coexistence and growth law characteristic can play a synergistic role in water treatment, CODcr, BOD5 and SS can be reduced, the content of the mud in the water is decreased, the decomposition of nutrient salts and deodorization are achieved.

The invention discloses a rice flower-promoting and rice-ear-protecting organic foliar fertilizer containing biological selenium and zinc and a preparation method thereof. The rice flower-promoting and rice-ear-protecting organic foliar fertilizer is characterized in that a nutrient solution is prepared from the following raw materials in percentage by weight: 10% of bone meal, 20% of onion leaves, 20% of oilseed rape residue, 10% of edible vinegar, 30% of water hyacinth, 10% of rhodospirillum extracting liquid and 0.1% of fermentation flora I. The preparation method comprises the following steps: crushing the onion leaves and the oilseed rape residue, feeding the fermentation flora I, performing high-temperature and high-speed solid-state fermentation and decomposition for 24 hours, taking out a fermented product, then adding the bone meal and edible vinegar for performing acetification, further adding water hyacinth fermentation liquid and rhodospirillum into acetified raw materials again and mixing and stirring uniformly. Selenium and germanium in the rice flower-promoting and rice-ear-protecting organic foliar fertilizer disclosed by the invention exist in biological enzyme modes, and the rice flower-promoting and rice-ear-protecting organic foliar fertilizer further contains active small molecule peptides; therefore, when the rice flower-promoting and rice-ear-protecting organic foliar fertilizer is sprayed on leaves of rice in blooming and ear setting periods, the piece weight of ear setting grouting of the rice can be promoted, the occurrence of rice aspergillus can also be prevented, the quality of products can be increased, and the function of reducing chalkiness degree can be achieved.

The invention discloses a lactic acid bacteria contained tomato cultivation medium and a preparation method thereof. The cultivation medium is characterized in that the medium comprises, by weight, 30% of rice hulls, 10% of sword bean vines, 20% of cucumber stems, 10% of rhodospirillum, 30% of river sand, 0.1% of fermentation microflora I and 0.1% of fermentation microflora II. According to the preparation method of the cultivation medium, agricultural organic wastes are smashed and subjected to solid state fermentation and decomposition for 24 hours at high temperature and high speed, and plant lactobacillus is added to perform uniform mixing. By means of the lactic acid bacteria contained tomato cultivation medium and the preparation method thereof, the cultivation medium is safe, environmental friendly, and poisonless and tasteless, seedling wilting and root rotting can not occur after direct usage, dinitrogenase, active amino acids and nutrients of plant somatomedin are contained, tomato root stock diseases such as root rot, damping off and sheath blight can be prevented, and aflatoxin produced during usage of the rice hulls can be removed.

The invention relates to the technical field of microorganisms, in particular to a microbial group and a microbial agent and application thereof. The microbial group comprises the following genus, a) nitrifying bacteria and b) nitrogen-fixing bacteria, specifically, the nitrifying bacteria comprise one of the genus xanthobacter, nitrobacter and nitrosomonas, and the nitrogen-fixing bacteria comprise one of the genus Klebsiella, Rhodospirillum, Azospirillum and Bayelinkia. The strains of the microbial group are reasonably and effectively matched, the microbial group can exist in a rhizosphere environment for a long time, the effects of high pesticide effect and lasting efficacy can be achieved, the strains of the microbial group promote and depend on one another, the nitrogen fixation effect of nitrogen-fixing bacteria is greatly improved, meanwhile, invalid nitrogen is changed into effective nitrogen, and the vegetative growth of crops is effectively promoted.

The invention discloses recombinant escherichia coli for producing pentamethylene diamine and application of the recombinant escherichia coli. A ribulose diphosphate carboxylase gene in rhodospirillum, a ribose phosphate kinase gene and a lysine decarboxylase gene in spinach are synthesized from the beginning, PCR copy an escherichia coli molecular chaperone gene to construct a plasmid pTrc99a-cadA-cbbM and a plasmid pCWJ-prkA-GroEL/GroES, the double-plasmid recombinant bacteria grow well in a specific culture medium, and glucose is efficiently utilized. According to the recombinant escherichia coli, inducers arabinose and IPTG are respectively added in different time periods, the heterologous gene expression is stable, the reaction system is simple, the condition is mild, the period is short, few byproducts are produced, the method is clean and pollution-free, the method is a simple, rapid and efficient production way, and the fermentation result shows that the yield of the recombinant escherichia coli KACCPG is obviously improved.

The invention relates to the technical field of compound fertilizer processing, in particular to a nano compound fertilizer and a preparation method thereof. The compound fertilizer is prepared from the following components in parts by weight: 1-3 parts of purple non-sulfur bacteria, 30-40 parts of urea, 20-50 parts of humic acid, 12-15 parts of sodium pyrophosphate, 5-10 parts of ammonium sulfate, 5-10 parts of corn straw, 20-35 parts of nano silicon dioxide, 15-20 parts of bran and 3-9 parts of rhodospirillum. The nutrient substances in the fertilizer can be slowly released, and loss of thenutrient substances is prevented.

The invention relates to a biological active enzyme composite microbial deodorizer. The biological active enzyme composite microbial deodorizer consists of a microbial agent, a plant extract, an alga extract, a biological active enzyme and water, wherein the microbial agent comprises 15-35% of lactobacillus plantarum, 15-35% of beer yeast, 2-6% of composite photosynthetic bacteria, 2-6% of improved denitrifying bacteria and 3-5% of rhodospirillum; the plant extract comprises 4-15% of a herba houttuyniae extract and 3-8% of a borneol extract; the alga extract is 0.5-2.5% of a spirulina extract; and the percentages are mass percentages. The biological active enzyme composite microbial deodorizer provided by the invention is low in cost, high in efficiency and wide in adaptation, is very practical in specific production, and greatly improves the efficiency of garbage disposal.

The invention relates to the technical field of microorganisms, and in particular relates to a Tistrella mobilis bacterial strain. The bacterial strain is H1, and the preservation number thereof is CGMCC No.6869. The invention also provides a bacterial strain biological pure culture which has all identification characteristics of the Tistrella mobilis bacterial strain H1 having the preservation number of CGMCC No.6869. The invention also provides a polycyclic aromatic hydrocarbon degradation reagent, and the polycyclic aromatic hydrocarbon degradation reagent includes the Tistrella mobilis bacterial strain or the biological pure culture. The invention also provides a soil remediation reagent, and the soil remediation agent includes the Tistrella mobilis bacterial strain or the biological pure culture. The Tistrella mobilis bacterial strain provided by the invention can effective degrade polycyclic aromatic hydrocarbons in the environment, and the scope of the microorganisms that can degrade the polycyclic aromatic hydrocarbons is enlarged.

The invention provides a compound microorganism denitrification fungicide. The compound microorganism denitrification fungicide comprises aspergillus niger, bacillus subtilis, nitrosomonas, nitrosospira, nitrospina, nitrococcus, pseudomonas aeruginosa, deaminopolycoccus, rhodopseudomonas palustris, rhodospirillum and gibberellins. The compound microorganism denitrification fungicide has the advantages that the reducing rate of ammonia nitrogen in water is 90%; the cost is low, the preparation process is simple, the water is purified, the products are degradable matter, and secondary pollutionto the environment is avoided.

The invention relates to a preparation method of a composite biological deodorant. The composite biological deodorant comprises a deodorant component and an extract product component according to a mass ratio of 1:1, wherein the deodorant component comprises, by mass, 20-40% of Lactobacillus plantarum, 20-40% of beer yeast, 1-5% of Rhodospirillum sp. and 1-5% of Bacillus subtilis; the extract product component comprises, by mass, 1-3% of lactic acid, 0.5-1% of citric acid, 0.02-0.05% of sodium benzoate, 1.5-2% of a natural plant extract product and 90-95% of water; and the natural plant extract product comprises, by mass, 10-20% of a Radix Sanguisorbae extract product, 20-30% of a mint extract product, 15-25% of a Lagerstroemia indica extract product and 15-20% of a sweet-scented osmanthus extract product. The composite biological deodorant has the advantages of low cost, high efficiency, wide adaptation and high practicality in concrete production.

The invention discloses a compound microorganism capable of efficiently degrading ammonia nitrogen. The compound microorganism is prepared from, in parts by weight, 30-50 parts of rhodospirillum, 20-40 parts of thiobacillus denitrificans and 10-20 parts of saccharomyces. The compound microorganism can effectively improve indexes such as ammonia nitrogen, nitrite and hydrogen sulfide in water bodies, improve self-cleaning capacity of the water bodies and purify water. The invention also discloses a preparation method and an application of the compound microorganism.

The invention relates to the technical field of maize straw application, in particular to a maize straw treatment method. Treated maize straw is used for repairing contaminated soil in a coal field mining area; the maize straw treatment steps include that maize straw is smashed, puffed, and mixed with a nutrient solution to inoculate a rhodospirillum bacteria solution, constant temperature incubation is carried out for four hours at 36 DEG C, and then the treated maize straw can be used; and the treated maize straw has the high absorption effect on copper (Cu), cadmium (Cd), chromium (Cr) and nickel (Ni), and is particularly suitable for repairing the contaminated soil in the coal field mining area.

The invention provides an application of Rhodospirillum rubrum in control of tumorous stem mustard clubroot and a tumorous stem mustard clubroot control agent. In specific application, Rhodospirillum rubrum is prepared into 1 to 2*10<8> cfu/mL bacterial suspension with water; before being transplanted into a field for culture, a tumorous stem mustard seedling is subjected to root cutting treatment; the root-cut tumorous stem mustard seedling is subjected to root soaking treatment for 30 to 60 min according to a manner that every 100mL bacterial suspension is used to treat 10 to 20 tumorous stem mustard seedlings. The tumorous stem mustard clubroot control agent comprises the Rhodospirillum rubrum which is taken as a main active component for controlling the tumorous stem mustard clubroot. Proved by experimental results, when the seedlings are treated by applying the Rhodospirillum rubrum and by adopting the specific application method provided by the invention, the finished-product rate when the tumorous stem mustard is harvested is improved by 12.5 percent, the disease index of the clubroot is reduced by 25.4 percent, and the control rate to the clubroot reaches 33 percent.

The invention belongs to the technical field of sewage treatment, and discloses a compound microorganism water purifying agent and an application thereof. The compound microorganism water purifying agent comprises a microorganism and a carrier, the microorganism comprises clostridium, saccharomycetes, micrococcus, acidobacteria, bacteroid, rhizobia, burkholderia cenocepacia, thiobacillus, opitutae, hydrogenophilales, lysobacter, brevundimonas, xanthomonas, comamonas, rhodocyclus and rhodospirillum, and the microorganism is fixed onto the carrier. The compound microorganism water purifying agent has high activity and is high in treatment efficiency, long-time domestication and selection is omitted, a short stable period is achieved in a treatment system, strains in the compound microorganism water purifying agent are cooperatively interact, utilization and metabolic decomposition of nutrient substances in water by the microorganism are facilitated, and COD (chemical oxygen demand) and ammonia nitrogen are efficiently reduced.

The invention discloses a natural circulation microbial agent for decomposing hydrogen sulfide and a preparation method of the natural circulation microbial agent. The microbial agent is prepared by adding strains into a fermentation liquid base solution for fermentation, wherein the strains include rhodopseudomonas, rhodospirillum, lactobacillus, candida, streptomyces, thiobacillus and xanthomonas, and the total bacteria density in the microbial agent is 6*10<8>-8*10<8> per liter. According to the microbial agent prepared by virtue of the preparation method, hydrogen sulfide can be effectively and rapidly decomposed, the microbial agent can be taken as a microbial deodorant, meanwhile, the growth of bacteria can be inhibited, the production of hydrogen sulfide gas is reduced, and meanwhile, the amount of marsh gas produced by the bacteria can be reduced; and the preparation method is simple, the preparation cost of the microbial agent is low, and the loss of living bacteria is greatlyreduced.

The invention discloses a photosynthetic type microbial preparation for aquaculture. The photosynthetic type microbial preparation for aquaculture is prepared from the following components in parts by weight: 8-10 parts of chroococcoid, 6-8 parts of oscillatoria, 6-8 parts of nostoc, 5-7 parts of anabaena, 4-5 parts of spiral seaweeds, 1-2 parts of rhodospirillum, 1-2 parts of rhodop seudanonas palustris and 1-2 parts of red micro bacteria. In this way, the photosynthetic type microbial preparation for aquaculture has the advantages of being complete in disinfection, cutting off viral infection, purifying water quality, achieving harmless standards, being natural and free of toxicity, increasing an aquaculture survival rate, and the like, and has a wide market prospect on popularization of the photosynthetic type microbial preparation for aquaculture.

The invention relates to the technical field of agricultural microorganisms, and particularly discloses a microbial agent for promoting growth of oilseed rape and an oilseed rape planting method thereof. The microbial agent provided by the invention comprises the following bacteria: a) at least one of corynebacterium, paracoccus, chromobacterium, achromobacter, acidithiobacillus, acidovorax, alcaligenes, arthrobacter, bacillus, cupriavidus, derxia, herbaspirillum, hydrogenobacter, hydrogenophaga, pseudomonas, pseudonocardia, rhizobium, rhodococcus, rhodopseudomonas, rhodospirillum, thiocapsa, xanthobacter, flavobacterium and wautersia; B) acetobacter, and c) azospirillum. The microbial agent provided by the invention improves the yield of oilseed rape, and is matched with a specific oilseed rape planting method to increase the yield of oilseed rape per mu, reduce the nitrogen fertilizer demand, reduce harmful germs, restore the number of microorganisms in soil and increase the diversity of the microorganisms.