68 results about "Herbaspirillum" patented technology

The invention provides five-alga probiotic enzyme tablets and a preparation method thereof. The five-alga probiotic enzyme tablets are prepared from the following raw materials in parts by mass: 10-20 parts of chlorella vulgaris, 1-10 parts of dunaliella salina, 1-10 parts of euglena, 1-10 parts of red algae, 10-20 parts of spirulina and 10-20 parts of probiotic powder. The five-alga probiotic enzyme tablets provided by the invention adopt the chlorella, dunaliella salina, red algae, spirulina and euglena for complementation in nutrition, and obtain trace elements easier to absorb by a human body through probiotic fermentation, thereby helping to reinforce the immunologic function of the human body, resist virus infection and proliferation, inhibit cancer cell proliferation, repair the damage of an organism quickly, remove vivotoxin, suppress blood pressure and blood sugar rise, reduce the serum cholesterol content, and thus improve the comprehensive conditioning of the human body.

The invention provides a mixed bactericide for restoring places polluted by polynuclear aromatic hydrocarbons, a preparation method thereof, belonging to the field of environment pollution restoration engineering. The strains used by the bactericide are Bordetella, Ochrobactrum, Rhodococcus, Achcromobacter, Herbaspirillum and Microbacterium which are mixed according to the ratio of 21-101:40-200:22-102:20-100:1:2 for fermentation to obtain a bacterial liquid, and the bacterial liquid is mixed with solid carriers according to the mass ratio of 0.5-1:1 to obtain a solid bactericide which has the effective living bacteria count of 108-1011/g. The mixed bactericide in the invention can effectively remove polynuclear aromatic hydrocarbons polluting soils and water, and has the advantages of high activity, rapid growth and propagation, strong adaptability, no generation of secondary pollution and the like.

The invention discloses a composite culture medium for fermentation of bifidobacteria and the manufacturing method, which comprises the following parts: 50-150g spirulina powder, 40-80g stachyose, 30-100ml 5% pork liver infusion, 1000ml distilled water. The effect of the invention is that: the infant bifidobacteria, adolescent bifidobacteria and long bifidobacteria grow well in the culture medium; the maximum increment reaches 1010cfu/ml in the best fermentation condition; the submerged culture effect is the best of the classic bifidobacteria selective culture medium, TPY culture medium and improved TPY culture medium. The composite culture medium provides a simple formulation, a low cost, a simple manufacturing process, which can be used to the research, exploitation, production of bifidobacteria, provides the important meanings of utilizing and developing spirulina and stachyose resource.

The invention discloses a breeding method of Spirulina alga species, which comprises the following steps: 1. selecting 5L of alga culture solution, filtering with a silk fabric to remove smaller algae; 2. carrying out mutagenesis with a phosphate buffer solution; 3. selecting single Spirulina with larger body and more spiral rings; culturing in a test tube, and calculating the growth rate; 4. culturing the Spirulina elements subjected to amplification culture in a 40001*12 DEG C lighting incubator; and 5. selecting the Spirulina element with the highest growth rate to obtain the mutant line subjected to mutagenesis screening, purification and cloning. The method changes the coarse breeding method of screening the alga species by the filter screen into capillary selected breeding; and the unicell screening is utilized to remove bad Spirulina individuals to obtain high purification, and the mutagenesis passivation is performed to obtain the high-quality high-growth-rate Spirulina alga species. The alga species purification and culture conditions are continuously optimized, so that the Spirulina yield is enhanced to 30-50%, and the product quality is further improved.

The invention refers to a method for reconstructing the cloned and constructed human body tumor death factor TRAIL gene expression carrier into spirulina gene group to be expressed stably with gene gun. The special components of the reconstructed TRAIL expression carrier include resistant mark gene expression box CAT, chlorophyll body photosynthetic gene promotor and ender, spirulina homologous segment, TRAIL gene segment without coding film combining part, the expression system also includes characters of primary nucleus and eukarya. The invention sets suitable striking parameter, uses gene gun to guide the reconstructed expression carrier into spirulina silk body, and through several times of sifting of chloramphenicol with incremental density and molecular biology testing, the invention gets spirulina converter of TRAIL gene. The invention applies to spirulina reconstruction and human body gene expression, it also applies to reconstruct and express other primary nucleolus and eukarya.

The invention relates to a method for biosynthesizing <15>N marked spirulina and a culture medium for realizing the method. The culture medium comprises a carbon source, a nitrogen source, a phosphorus source, inorganic salt, and trace elements; moreover, <15>N urea is used as the nitrogen source; and dextrose and sodium bicarbonate are used as the carbon source. The method of the invention comprises enriching and acclimating the species of the <15>N spirulina, inoculating the species of the <15>N spirulina in the culture medium for culturing to obtain culture liquid containing the <15>N marked spirulina; and separating the <15>N marked spirulina from the culture liquid. The method also comprises recycling of the culture liquid. The method can obtain the <15>N marked spirulina with high abundance ratio; moreover, the yield is high and the cost is low.

The invention provides a purification and breeding method of a spirulina species. The method comprises the following steps: (1) preparing 3L of a spirulina species culture solution; (2) filtering the culture solution; (3) transferring a spirulina gel, which is obtained from filtration, into a triangular flask, adding 200-300ml of medium L6, conducting adaptive restoration culture, and controlling environments as follows: illumination is 4000lx and temperature is 35 DEG C; (4) conducting alkaline cleaning on the spirulina gel; (5) cleaning the spirulina gel; (6) reserving supernatant spirulina of liquid, adding 50-100ml of medium L6, and uniformly mixing the materials; (7) diluting an obtained mixture; (8) transferring 1ml of the liquid to a sterilized 1-5ml centrifuge tube, and conducting adaptive restoration culture on the centrifuge tube in a water bath of 2000lx and 30 DEG C for 12h; and (9) selecting large individual spirulina having a relatively high amount of spirals by virtue of a single-filament separator, and conducting enlarge cultivation on the large individual spirulina having a relatively high amount of spirals so as to achieve the large-scale production of the spirulina species. With the application of the method, by effectively conducting multi-stage enlarge cultivation on single-filament algae, pure species free from miscellaneous bacteria or miscellaneous algae can be obtained.

The invention relates to the development and application technology of spirulina, and aims to provide a method for identifying spirulina strains with favorable production traits. The method provided by the invention comprises the steps of enhancing genomic DNA (deoxyribose nucleic acid) of a candidate strain by a special primer HIPS-TC, and establishing a dendrogram of the spirulina strains by adopting NTSYpc2.1 software after electrophoretic analysis. If the candidate strains have 1130bp, 620bp, 580bp and 540bp special DNA electrophoretic bands, and can be compounded with the existing strain with favorable production traits, the production traits of the candidate strains are favorable, and are suitable for large scale cultivation and production. If the candidate strains have 1190bp, 780bp, 580bp,520bp, and 470bp special DNA electrophoretic bands, and can be compounded with the existing strain with poor production traits, the production traits of the candidate strains are poor, and are not suitable for large scale cultivation and production. Compared with the common method, the method for identifying spirulina strains with favorable production traits in the invention has the advantages of simpleness, fast speed, efficiency and low cost, and is suitable for large-scale and high throughput screening.

Abstract of Disclosure A composition for pharmaceutical products and cosmetic products contains IgY egg yolk antibodies of SPF hens and microalgae extracts from Spirulina species, Spirulina varieties, and Chlorella species. The composition can also contain oxytetracycline and preservatives or antioxidants. The composition prepared as a salve is used for healing infected wounds and for preventing the infection of wounds.

The invention discloses bacillus subtilis feed for crabs, which is characterized by being prepared from the following raw materials in parts by weight: 13-15 parts of chrysanthemum, 23-28 parts of cottonseed meal, 11-13 parts of coffee bean, 2-4 parts of calcium carbonate, 9-13 parts of bacillus subtilis, 17-19 parts of spiral seaweed, 5-8 parts of palm oil, 3-5 parts of chicken oil, 15-18 parts of cuttlefish, 21-30 parts of chicken meat, 18-20 parts of water hyacinth, 12-14 parts of green pepper, 14-18 parts of watermelon, 1-2 parts of pericarpium citri reticulatae, 13-17 parts of cushaw stem, 0.3-0.6 part of field pennycress, 2-4 parts of platycodon grandiflorum, 3-6 parts of a phagostimulant and a defined amount of water. The feed provided by the invention is high in nutritive value, safe and reliable to use, low in production cost and easy to popularize.

The invention discloses a hydrangea health jelly, which is composed of the following raw materials in parts by mass: 10-15 parts of hydrangea, 1-3 parts of hazelnuts, 1-2 parts of sesame, 1-4 parts of hawthorn, and 1-2 parts of shiitake mushrooms , 3 to 6 parts of spirulina, 3 to 5 parts of fructooligosaccharides, 1 to 3 parts of calcium lactate, 3 to 5 parts of gelling agent, 20 to 25 parts of flavoring agent, and 200 parts of water. In the present invention, hydrangea, spirulina, shiitake mushroom, hazelnut, sesame, hawthorn, calcium lactate, fructooligosaccharide, etc. are compounded, and the calcium content is high, which can promote calcium absorption, prevent calcium deficiency, and also has the function of strengthening the spleen. The health-care jelly of the present invention has the functions of appetizing, helping digestion, reducing blood pressure and lipid, anti-cancer, anti-tumor, and enhancing immunity.

To provide a technology capable of promoting growth of Bifidobacterium bacteria and lactic acid bacteria under an environment causing the growth or no change in cell count while suppressing decrease under an environment causing the decrease. Provided is an agent for promoting the growth of, and/or suppressing a decrease in, Bifidobacterium bacteria and/or lactic acid bacteria containing Arthrospira blue-green algae and/or Spirulina blue-green algae as an active ingredient. Also provided is an oral composition including Arthrospira blue-green algae and/or Spirulina blue-green algae together with at least one type of bacteria selected from the group consisting of Bifidobacterium bacteria and lactic acid bacteria. Furthermore provided is a method for promoting growth of Bifidobacterium bacteria and/or lactic acid bacteria and/or suppressing the decrease including a step for allowing at least one type of bacteria selected from the group consisting of Bifidobacterium bacteria and lactic acid bacteria to coexist with Arthrospira blue-green algae and/or Spirulina blue-green algae.

The present invention discloses the application of L-arginine, L-ornithine and L-citrulline as spirulina princeps growth regulator, and aims at promoting the growth of spirulina princeps for cultivating spirulina princeps, L-arginine, L-ornithine or L-citrulline is added into culture liquid to speed the growth of spirulina princeps, raise the yield of spirulina princeps. Test shows that adding L-arginine, L-ornithine or L-citrulline in 0.2-5 g/L into the culture liquid can raise the yield of spirulina princeps by 8-52 % and speed the growth of spirulina princeps by 10-20 %.

The invention provides lactation-promoting sow feed and a preparation method thereof. The formula of the feed is prepared from 20 to 40 parts of corn kernels, 5 to 25 parts of dried small shrimp powder, 10 to 30 parts of soybean meal, 12 to 28 parts of whey powder, 15 to 27 parts of spirulina, 9 to 21 parts of duckweed, 12 to 24 parts of nigella damascene, 8 to 24 parts of melodinus fusiformis leaf, 6 to 18 parts of dregea sinensis vine, 12 to 24 parts of primula cernua franch rhizome, 9 to 21 parts of stem of October clematis, 6 to 22 parts of helwingia japonica stem pith and 0.5 to 2 parts of probiotic bacteria. The preparation method of the feed comprises the following steps: (1) fermenting the corn kernels, the soybean meal and the whey powder; (2) reflowing and steaming the spirulina,the duckweed, the nigella damascene, the melodinus fusiformis leaves, the dregea sinensis vines, the primula cernua franch rhizomes, the stems of October clematis and the helwingia japonica stem pithby utilizing acetic acid; (3) mixing all the materials and carrying out secondary fermentation. The feed provided by the invention has the effects of clearing away heat and promoting lactation, dispelling wind and dampness, diminishing swelling and diminishing inflammation, enhancing the body constitution and the like.

The invention discloses a dried-ivy mosses nutritive cordial and a preparation method thereof. The dried-ivy mosses nutritive cordial is prepared from the following raw materials in parts by weight: 50-90 parts of dried ivy mosses, 15-25 parts of undaria pinnatifida, 10-15 parts of white hyacinth bean, 8-13 parts of euryale ferox, 8-12 parts of spirulina, 20-40 parts of Chinese liquor, 3-4 parts of matrimony vine, 1-2 parts of polygonatum sibiricum, 2-3 parts of galium aparine, 1-3 parts of radix astragali, 2-3 parts of sargentodoxa cuneata, 1-2 parts of herba artemisiae scopariae, 1-2 parts of angelica dahurica, 5-9 parts of tilia honey, 0.02-0.25 part of high fructose syrup, 0.05-0.35 part of rose pure syrup and proper amount of water. The dried-ivy mosses nutritive cordial is abundant in nutrition and health components of dried ivy mosses, is refreshing, fragrant, sweet and palatable; by adding components such as undaria pinnatifida, spirulina and the like into the formula, the nutrition and health functions are increased; and by adding components such as radix astragali, polygonatum sibiricum and the like which have the effects of resisting fatigue and resisting aging, the dried-ivy mosses nutritive cordial is capable of effectively improving human body functions, building up body and helping to keep full energy.

The invention discloses a purification and renovation method for species of spirulina, which belongs to the technical field of species culture of spirulina. The method comprises the following steps: 1)preparing a nutrient solution; 2)taking spirulina slurry for cell disruption, adding the nutrient solution after 3-10 minutes, wherein the weight ratio of spirulina slurry to the nutrient solution is 1: 8-12; 3) continuously using ultraviolet light for irradiation for 2-5 days, wherein irradiation time per day is 1-2 hours, and the temperature of culture water is 25-35 DEG C; and 4)adding an auxiliary nutrient solution for culture for 5-10 days after the next day of completion of the ultraviolet light irradiation. The method has the advantages of simple operation and low cost, can increase the spirulina output, and can be used for culturing species of spirulina.

The invention relates to the technical field of agricultural microorganisms, and particularly discloses a microbial agent for promoting growth of oilseed rape and an oilseed rape planting method thereof. The microbial agent provided by the invention comprises the following bacteria: a) at least one of corynebacterium, paracoccus, chromobacterium, achromobacter, acidithiobacillus, acidovorax, alcaligenes, arthrobacter, bacillus, cupriavidus, derxia, herbaspirillum, hydrogenobacter, hydrogenophaga, pseudomonas, pseudonocardia, rhizobium, rhodococcus, rhodopseudomonas, rhodospirillum, thiocapsa, xanthobacter, flavobacterium and wautersia; B) acetobacter, and c) azospirillum. The microbial agent provided by the invention improves the yield of oilseed rape, and is matched with a specific oilseed rape planting method to increase the yield of oilseed rape per mu, reduce the nitrogen fertilizer demand, reduce harmful germs, restore the number of microorganisms in soil and increase the diversity of the microorganisms.

The invention discloses high-effectiveness healthcare oral liquid. The high-effectiveness healthcare oral liquid is prepared from, by weight, 5-10 parts of tricholoma matsutake mycelium, 3-5 parts of grifola frondosa mycelium, 5-8 parts of antrodia mycelium, 2-3 parts of lucid ganoderma mycelium, 10-25 parts of rhizoma atractylodis macrocephalae, 15-20 parts of radix codonopsis, 6-9 parts of spirulina and 8-12 parts of green tea. The invention further discloses a method for preparing the high-effectiveness healthcare oral liquid. According to the method, rhizoma atractylodis macrocephalae, radix codonopsis, spirulina and green tea are extracted and cooled naturally, then wall-broken tricholoma matsutake mycelium, grifola frondosa mycelium, antrodia mycelium, lucid ganoderma mycelium and fermentation liquor are added, and finally uniform mixing is conducted to obtain the high-effectiveness healthcare oral liquid. The oral liquid has an efficient radiation resisting effect, and is especially suitable for people working in the fields of guided missile and radar and in nuclear submarine troops where radiation hazard is serious.

The invention discloses a nutrient solution for the domestic culture of spirulina. In the traditional culture of the spirulina, fresh water culture or seawater culture is adopted, the obtained spirulina is called fresh water spirulina or seawater spirulina, and the two culture modes both need a culture pool which is suitable for the growth of the spirulina. However, the domestic culture of the spirulina enjoys extensive development space. The invention provides the nutrient solution suitable for the domestic culture of the spirulina. The nutrient solution is prepared from fresh water, inorganic salt and trace elements. The invention is mainly used for the domestic culture of the spirulina, can regulate the growth rule of the spirulina aiming at the physiological characteristic of the spirulina, promotes the growth and the development of the spirulina, improves the product quality and enhances the product yield.

The invention discloses a complex bacterium fermentation substrate for improving the transplanting survival rate of soft-seeded pomegranate young seedlings. The complex bacterium fermentation substrate is prepared from the following ingredients in parts by weight: 220 to 222 parts of spirulina, 46 to 47 parts of nanometer bentonite, 51 to 52 parts of mesoporous pearl powder, 90 to 92 parts of brown sugar slag, 78 to 80 parts of bagasse, 67 to 68 parts of pumpkin residua, 71 to 72 parts of sesame cake, 34 to 35 parts of chitooligosaccharides, 7 to 8 parts of bacillus subtilis, 8 to 9 parts of lactobacillus, 21 to 22 parts of humic acid, 22 to 23 parts of matrine and the like. The spirulina is used; the ultra-fine brown sugar slag, bagasse, pumpkin residua and sesame cake are used; the fruit tree transplanting substrate is prepared through enzymolysis, fermentation and nanometer reinforcement; the vitality of the root system of the transplanted fruit trees can be promoted; the root system soil environment of the transplanted fruit trees can be improved; meanwhile, the used matrine can achieve the effect of improving the root system resistance of the fruit trees; the effect of improving the survival rate of the soft-seeded pomegranate young seedlings can be comprehensively achieved.

The invention discloses an insecticidal composition for treating parasitic chironomidae, ephydrid larva, blood worm, and rotifer during a spirulina cultivation process. The insecticidal composition iscomposed of sodium bicarbonate, ammonium bicarbonate, calcium chloride, chlorine dioxide, sodium hyposulfite, citric acid, sodium selenite, and fulvic acid according to a mass ratio being 1: 0.1-0.2:0.1-0.15: 0.1-0.15: 0.08-0.1: 0.01-0.03: 0.01-0.03: 0.1-0.2. The insecticidal composition has no toxic and side effect, has good insecticidal effect, cannot remain in cultured objects, and thereby puts an end to food chain pollution. The insecticidal composition can rapidly and efficiently process insect pest, can increase spirulina cultivation capacity by 30%, improves a traditional cultivationmethod, and reduces the high cost of traditional cultivation.