56 results about "Endophytic bacteria" patented technology

The invention relates to the technical field of agricultural microorganisms. The invention provides a compound microorganism bacterium suspension, a bacterium agent, a tobacco seed coating agent as well as a preparation method and application of the compound microorganism bacterium suspension. The compound microorganism bacterium suspension comprises a rice seed endophytic bacterium spiraceae R3 bacterium suspension and a rice seed endophytic bacterium stenotrophomonas R5 bacterium suspension; the mass ratio of R3 to R5 is 1: (0.5-1.5); the endophytic bacterium herbapiril R3 of the rice seeds is Herbapiril sp. R3, and the preservation number of the herbapiril R3 is CCTCC (China Center For Type Culture Collection) NO: M 20231757; the rice seed endophytic bacterium stenotrophomonas R5 is Shenotrophomonas sp. R5, and the preservation number of the rice seed endophytic bacterium stenotrophomonas R5 is CCTCC (China Center for Type Culture Collection) NO: M 20231758. The compound microbial suspension is used as a seed coating agent to coat tobacco seeds, and growth of tobacco root systems can be remarkably promoted.

The invention relates to the technical field of phytoremediation of Cd-polluted soil. The invention provides Cd-resistant bacillus cereus as well as application and a method of the Cd-resistant bacillus cereus in remediation of Cd-polluted soil, the preservation name of the bacillus cereus is Bacillus cereus 01, the Latin name of the bacillus cereus is Bacillus cereus, the bacillus cereus is preserved in Guangdong Microbial Culture Collection Center, the preservation number of the bacillus cereus is GDMCC No: 65570, and the preservation time is December 2, 2024. The efficient Cd-resistant endophytic bacteria are obtained through screening and identification, the Cd-resistant endophytic bacteria are combined with the smooth vetch to repair Cd-polluted soil, absorption of succeeding crops to Cd is reduced, the food safety problem caused by standard exceeding of Cd is reduced, and the method has the advantages of being low in cost, high in repair efficiency, simple and convenient in treatment step and the like.

The invention discloses a Klebsiella Michaeli MX-56 strain and application thereof, and belongs to the technical field of biological agriculture. The Klebsiella michelianensis MX-56 disclosed by the invention is preserved in the Guangdong Microbial Culture Collection Center, the preservation number is GDMCC No: 66361, the preservation time is May 19, 2025, and the preservation address is No. 100, Xianlie Middle Road, Guangzhou City. The invention also discloses an application of the Klebsiella michelianensis MX-56 in preparation of a divaricate saposhnikovia root growth promoting agent and a method for promoting growth of divaricate saposhnikovia root by using the Klebsiella michelianensis MX-56. A root irrigation method is adopted to irrigate MX-56 bacterial liquid. The divaricate saposhnikovia root endophytic bacterium Klebsiella michelianensis MX-56 disclosed by the invention has the growth promoting characteristics of fixing nitrogen, dissolving inorganic phosphorus, and secreting IAA (Indoleacetic Acid) and an iron-producing carrier; the strain can be stably colonized in the soil around the divaricate saposhnikovia roots; a good growth promoting effect is achieved on wind prevention.

The invention provides hypnum melioides endophytic bacteria with a drought resisting effect and application thereof, and belongs to the technical field of microorganisms. The endophytic bacterium of the grimaria mellea provided by the invention is prelisteria aryabhattai Par-6, the strain is preserved in the China General Microbiological Culture Collection Center, the address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is August 29, 2025, and the preservation number is CGMCC NO.35771. The endophytic bacterium of the grimaria mellea provided by the invention has the advantages that the endophytic bacterium of the grimaria mellea can be used for preparing the endophytic bacterium of the grimaria mellea; the endophytic bacteria of the grimaria mellea provided by the invention can significantly improve the tolerance of plants under drought conditions.

The application discloses application of animal endosymbiont in assisting corals to resist heat stress. The application significantly improves the resistance of corals to high-temperature environment by applying the animal endosymbiont SCSIO 12664, and helps to protect the corals from the influence of heat stress. The application introduces the application of the animal endosymbiont SCSIO 12664, and the technical scheme aims to overcome the defects of the prior art, improve the resistance of corals to high-temperature heat stress, reduce the negative influence on the ecosystem, and achieve the goal of more sustainable and effective protection of corals.

Endophytic bacteria, compositions comprising the same, and methods of use thereof are disclosed which suppress growth of aggressive, invasive weed species which grow in the same habitat of target plants of interest.

The invention provides a bacillus velezensis strain and application thereof. The bacillus velezensis strain Amzn015 is firstly separated from the interior of a healthy bighead atractylodes rhizome plant, and the preservation number of the strain is CCTCC M 20252027. The bacterial strain has good antagonistic activity on Atractylodes macrocephala koidz root rot pathogen fusarium oxysporum, Fusarium oxysporum pathogen and Epicoccus catenarius, and the inhibition rates of the bacterial strain and the Fusarium oxysporum pathogen, the Fusarium oxysporum pathogen and the Epicoccus catenarius are respectively 85.75%, 76.43% and 72.11%. The bacterial strain has good antagonistic activity on Atractylodes macrocephala koidz root rot pathogen, the Fusarium oxysporum pathogen, the Fusarium oxysporum pathogen, the Fusarium oxysporum pathogen and the Epicoccus catenarius. The strain has a good treatment effect on bighead atractylodes rhizome root rot caused by fusarium oxysporum, the treatment effect is 66.2%, the strain is a potential biological agent, and the strain also has the capability of promoting plant growth. The bacillus velezensis disclosed by the invention has a wide development space and a good application prospect.

The application discloses a broad-spectrum growth promoting bacterial agent combination for oil camellia and oil tea, a biological preparation and application thereof, and relates to the field of microbial technology.The endophytic bacteria are separated from healthy oil camellia plants, the abilities of the strains in dissolving phosphorus, fixing nitrogen, producing IAA, producing iron carrier and antagonizing the pathogenic bacteria of oil tea anthracnose are determined, the onion Burkholderia and Aspergillus niger with excellent comprehensive growth promoting functions are screened to prepare a combined bacterial agent, and the combined bacterial agent is verified to have a significant growth promoting effect on the oil camellia and the oil tea through a potting experiment.The combined bacterial agent promotes the growth, nutrient absorption and root development of the oil tea, significantly improves the enzyme activities related to the carbon, phosphorus and nitrogen cycles of the soil, and realizes the overall optimization of the "plant-soil" system.The application finds a broad-spectrum mixed bacterial agent suitable for the artificial planting of the oil camellia and the oil tea in a rocky desertification area, effectively improves the adaptability of the plants in the rocky desertification habitat, and provides a new microbial technical approach for the collaborative repair of multiple tree species in the area.

The application provides a camellia plant endophytic bacteria, a bacterial agent thereof and an anti-influenza virus A application; the camellia plant endophytic bacteria is Bacillus thuringiensis, which is preserved in the China General Microbiological Culture Collection Center with a preservation number of CGMCC No.32945. The fermentation product of the strain shows good antiviral activity in in vitro and in vivo experiments, can effectively inhibit the replication of influenza virus, and protect infected mice from lethal influenza virus; also shows the effect of regulating immune response, enhances the secretion of IFN-gamma by down-regulating the secretion of pro-inflammatory factors such as IL-6 and IL-10, thereby improving the immune resistance of the body.

This invention belongs to the field of microbial technology and discloses a synthetic microbial community for enhancing biological nitrogen fixation in karst grasslands, its construction method, and its application. The synthetic microbial community includes... Sinorhizobium americanum , Sionrhizobium sp. as well as Bacillus cereus Wherein: the Sinorhizobium americanum The accession number is GDMCC NO: 67796; Sionrhizobium sp. The accession number is GDMCC NO: 67797; Bacillus cereus The accession number is GDMCC NO: 67798. For leguminous forage grasses that require repeated sowing, the seeds are treated with a synthetic microbial community before being sown in the field; for perennial leguminous forage grasses that have already been planted, the synthetic microbial community is diluted with water and sprayed onto the surface of adjacent leguminous forage grasses. This invention screens out suitable and efficient root nodule endophytic bacteria in karst areas and constructs a synthetic microbial community to enhance the biological nitrogen fixation of leguminous forage grasses. It clarifies the technical details of the practical application of the synthetic microbial community in the field. The application of this technology can significantly improve the biological nitrogen fixation rate of leguminous forage grasses, significantly reduce the amount of nitrogen fertilizer used in forage grasses, and significantly increase forage grass yield.

The present application belongs to the field of traditional Chinese medicine microbial technology, and particularly relates to a method for increasing the content of rapeseed pollen polysaccharide. The method is to increase the content of rapeseed pollen polysaccharide through microbial fermentation, and the microorganism is selected from plant endophytic bacteria and known microbial flora. The microorganism can be a single flora or a mixed flora. The microbial fermentation method can significantly increase the content of plant medicine polysaccharide, create a product probiotic environment, improve the pharmacological activity, and can be widely used in the deep fermentation production of plant medicines.

The invention relates to the technical field of microorganisms, in particular to an atractylodes lancea endophytic bacterium ALX01 and application thereof, the atractylodes lancea endophytic bacterium ALX01 is named as Pseudomonas sp. ALX01, the preservation number is CCTCC NO: M20252198, the preservation place is China Center for Type Culture Collection, Wuhan University, Wuhan, China, on October 11, 2025, and the preservation number is CCTCC NO: M20252198. By utilizing the atractylodes lancea endophytic bacterium ALX01, the problem that high-content and high-stability production of aceratrol and beta-eudesmol in non-genuine production areas is difficult to realize in the prior art is solved.

This disclosure relates to biofertilizers. In particular, this disclosure relates to combinations and compositions of biofertilizers containing endophytic bacteria and mycorrhizae, as well as methods of using them.

The present invention provides a method for removing endophytic bacteria of the genus Curtobacterium from tissue-cultured lily seedlings, comprising the following steps: 1) excising the contaminated leaves and bulb discs from the tissue-cultured seedlings; 2) placing the tissue-cultured seedlings obtained in step 1) into a mixed solution containing trichloroisocyanuric acid with a mass concentration of 0.5% and sodium dodecyl sulfate with a mass concentration of 0.5% and with a mass ratio of 1:1, and soaking for 10-15 min; 3) rinsing 3 times with sterile water; 4) inoculating the tissue-cultured seedlings onto a culture medium of MS + 50 mg / L streptomycin sulfate; 5) placing the tissue-cultured seedlings in an incubator at a temperature of 22±1°C and a light duration of 10-14 h / d, and culturing for 2-3 weeks to observe the contamination of endophytic bacteria of the genus Curtobacterium; 6) if there is no contamination, continue culturing for 2 weeks, and inoculate the tissue-cultured seedlings into a proliferation culture medium of MS + 6-BA 1.0 mg / L + NAA 0.5 mg / L for culturing; 7) if there is contamination, repeat steps 1)-4) to remove the endophytic bacteria of the genus Curtobacterium. The bacteriostatic rate can reach over 90%.

This invention discloses a strain of Codonopsis pilosula endophytic fungus that produces extracellular polysaccharides and its applications. This Codonopsis pilosula endophytic fungus is a short-spore bacterium, named... Brevibacillus schisleri CpRG-1. The Codonopsis pilosula endophytic bacteria of the present invention exhibit excellent ability to produce extracellular polysaccharides, producing high concentrations of extracellular polysaccharides, which can greatly reduce the cost of extracting polysaccharides from the medicinal herb Codonopsis pilosula; at the same time, the extracellular polysaccharides produced by the Codonopsis pilosula endophytic bacteria not only have antibacterial and hypoglycemic activities, but also can promote the healing of diabetic foot ulcers and improve the intestinal microbiota, providing new ideas for the development of antibacterial drugs, diabetes prevention foods and drugs, and diabetic foot ulcer treatment foods and drugs.

The present invention discloses an endophytic bacterium R3 of Actinidia arguta and its application in promoting plant growth, which relates to the fields of bioinformatics, agronomy, and microbiology. Specifically, by analyzing the environmental factors of the sampling sites of Actinidia arguta and the community structure of root endophytic bacteria, and combining with the classical microbial isolation and culture methods, an endophytic bacterium R3 of Actinidia arguta that can promote plant growth is screened out. The endophytic bacterium R3 of Actinidia arguta significantly increases the total chlorophyll content, glutamine synthetase activity, and auxin content of maize, and at the same time significantly increases the plant height, root length, and biomass of maize.

The invention belongs to the technical field of ligusticum wallichii root rot resistance, and particularly discloses a ligusticum wallichii endophytic composite flora and application thereof. According to the present invention, the composite bacterial flora comprises Bacillus subtilis, Fusarium acuminatum (Fusarium acuminatum) and Purpureocillium lilacinum (Purpureocillium lilacinum), and the composite bacterial flora comprises Bacillus subtilis, Fusarium acuminatum (Fusarium acuminatum) and Purpureocillium lilacinum (Purpureocillium lilacinum (Purpureocillium lilacinum (Purpureocillium lilacinum), the composite flora can be stably colonized in ligusticum wallichii tissue culture seedlings and continuously exerts the antagonistic effect on root rot, so that public nuisance chemical prevention and treatment agents are replaced. The method has the advantages that endophytes of ligusticum wallichii are reasonably combined, so that the colonization instability and insecurity caused by direct field application of a conventional exogenous biocontrol microbial inoculum are avoided; and meanwhile, a simplified ligusticum wallichii micro-ecosystem is constructed, and the function and the structure of the ligusticum wallichii micro-ecosystem are more stable by adopting a cross-boundary combination of multiple strains of bacteria and fungi.

The invention discloses a morinda citrifolia endogenous bacterium isolation medium and a preparation method thereof. According to the method, the fresh morinda citrifolia or a combination of the fresh morinda citrifolia and the morinda citrifolia fermentation waste is used as an effective component of the separation culture medium, nutrition and environment closer to the natural habitat of the morinda citrifolia are provided, the endogenous bacteria of the morinda citrifolia can be effectively separated and cultured, and the separation success rate of the endogenous bacteria is increased. In addition, fermentation waste residues of the morinda citrifolia enzyme industry are directly used as core components of the morinda citrifolia endogenous bacterium isolation culture medium, high-valued cyclic utilization of waste is achieved, and the raw material cost is reduced. Meanwhile, the preparation process of the isolation culture medium is simple and easy to operate, and a foundation is laid for large-scale industrial production.

The invention relates to a soybean root nodule endogenous fomesafen degrading bacterium and application thereof. The classification name of the fomesafen degrading bacterium is Mycobacterium sp. W109, and the preservation number of the fomesafen degrading bacterium is CCTCC (China Center for Type Culture Collection) NO: M 20251729. According to the invention, a fomesafen degrading bacterium is separated from soybean root nodules, the bacterium can stably degrade fomesafen, and the variety of the fomesafen degrading bacterium is expanded.

The present application belongs to the field of biological control, and particularly relates to a synthetic bacterial community of Ligusticum chuanxiong Hara endophytic bacteria, uses thereof, a plant protection agent for preventing and treating root rot, and a method for cultivating disease-resistant seedlings. Plantibacter flavus (P36-15), Microbacterium schleiferi (P48-28), Rothia aeria (P48-75), are proportionally compounded, inoculated into Ligusticum chuanxiong Hara seedlings, can prevent root rot caused by Fusarium oxysporum and Fusarium solani, improve the ability of Ligusticum chuanxiong Hara plants to resist root rot, improve the field control effect and medicinal material yield for preventing and controlling Ligusticum chuanxiong Hara Fusarium oxysporum and Fusarium solani type root rot, and have popularization and application value.

The application relates to a Chryseobacterium sp. with a preservation number of CGMCC No. 25365 and a strain number of CP3; the Chryseobacterium sp. has the characteristics of promoting growth and degrading chlorpyrifos, can be prepared into a bacterial agent, can enhance the degradation of residual chlorpyrifos in rice, can promote the growth of rice, and is applied to the repair of chlorpyrifos residual pollution in a rice planting system; the Chryseobacterium sp. belongs to plant endophytic bacteria, can long-term colonize in rice, can reduce pesticide residues in crops on the premise of not affecting the quality of crops, can guarantee the quality and safety of agricultural products, and can realize green and safe production.

The invention belongs to the technical field of aquaculture science, microbiology, immunology and bioengineering, and particularly relates to application of endophytic monad in serving as disease-resistant probiotics of shellfish. The added endophytic monad has a protection effect on the pathogen infected shellfish, the survival rate of the shellfish can be remarkably increased by adopting a seawater direct adding method of the two endophytic monad, namely a pre-adding method and a continuous adding method, and although the two tested adding methods have remarkable protection effects, the survival rate of the shellfish can be remarkably increased. However, compared with a continuous adding method, the pre-adding method is simpler, more convenient and more labor-saving in operation, and the pre-adding method is more recommended to be adopted in practical application; the principle for realizing protection is related to the growth inhibition of the endophytic monad on pathogenic bacteria, the reduction of host shellfish tissue damage and the improvement of the phagocytic activity of host shellfish blood cells. The invention provides a new strategy for improving disease resistance of shellfish and reducing disease loss of shellfish aiming at the current situations of difficulty in disease prevention and control and antibiotic abuse in current aquaculture, and can be applied to scenes such as healthy culture of shellfish and the like.

The present invention belongs to the technical field of Chinese medicinal material seedling cultivation, and specifically relates to a method for direct seeding of Bletilla striata seedlings using endophytic bacteria thereof, comprising obtaining Bletilla striata seeds, sowing, seedbed management, transplanting and fostering the seedlings; the nutrient soil on the seedbed is composed of a bacterial bottom matrix and a bacterial top matrix; first, a bottom matrix with a thickness of 6 to 7 cm is laid on a non-woven fabric; a Bacillus velezensis liquid is sprayed on the bottom matrix; then, clean water is sprinkled and stirred evenly until a bacterial bottom matrix with a moisture content of 60 to 70% is obtained; a Bacillus velezensis liquid is sprayed on the top matrix, then, clean water is sprinkled and stirred evenly until a bacterial top matrix with a moisture content of 60 to 70% is obtained; the bacterial top matrix is laid on the bacterial bottom matrix to a thickness of 5 to 6 cm, and then compacted to obtain the nutrient soil. The method of the present invention can ensure the survival rate of Bletilla striata seedlings after transplanting and enhance their competitiveness with field weeds.

The invention discloses a method for purifying C21 steroidal glycoside in fermentation broth, which belongs to the technical field of substance purification, and comprises the following steps: taking radix cynanchi bungei endophyte with C21 steroidal glycoside synthesis capability as a fermentation strain, and inoculating the fermentation strain to an LB culture medium or a Gause No.1 culture medium for activation; then transferring into a fermentation culture medium and culturing to a secondary metabolism period; centrifuging the fermentation liquor at 10,000 rpm for 5 minutes, collecting supernate, drying to obtain a precipitate, adding absolute ethyl alcohol, uniformly mixing, standing for 10 minutes, centrifuging, and collecting supernate alcohol extract; adding the alcohol extract into a precipitating agent according to a volume ratio of 1: (1-7), oscillating and uniformly mixing, standing overnight, centrifuging, and collecting a precipitate; and the precipitate is dried, such that the C21 steroidal glycoside is obtained. The method does not need to depend on plant resources, is high in sustainability, repeatability and stability, is suitable for large-scale production, and has important significance in the technical field of purified substances and the field of medicines.

The invention belongs to the field of plant tissue culture, and particularly relates to a bacteriostatic agent for tissue culture of snow white water lily as well as a preparation method and application of the bacteriostatic agent. The bacteriostatic agent comprises an azoxystrobin suspending agent, carbendazim and mancozeb wettable powder. The bacteriostatic agent consists of a plurality of effective bacteriostatic components, can effectively control the growth of endophytes of the snow-white water lily, reduces the fungal infection rate of the snow-white water lily, and has an inhibition effect on different endophytic fungi. In the tissue culture process, after the bacteriostatic agent is used, the contamination rate of the tissue in the culture process can be remarkably reduced, the normal operation of the tissue dedifferentiation process is effectively ensured, and the success rate of the tissue culture is improved.

The invention discloses a low-temperature low-carbon water extraction-natural fermentation debitterizing method of Huyoshan Huyoshan pomelo, which comprises the following steps: extracting Huyoshan pomelo peel by using water as an extraction medium in combination with a physical superfine grinding and ultrasonic-assisted technology by adopting a water extraction method, so as to realize efficient extraction of naringin; then, further debitterizing by utilizing a natural fermentation debitterizing mode, specifically, placing an extracted clear solution subjected to ultrasonic treatment in a sealed environment, fermenting by utilizing an endophytic flora naturally attached to the grapefruit peel, degrading and converting a bitter substance naringin by virtue of microbial metabolism and an endogenous enzyme effect, and performing centrifugal separation after natural fermentation is completed, and supernate is removed to obtain debittered grapefruit peel powder, so that the bitter taste strength is further reduced, and the flavor is improved. The method has the advantages of being environmentally friendly, low in temperature, low in carbon, remarkable in debitterizing effect and the like, and the obtained extracting solution and the debitterized grapefruit peel powder both keep certain antioxidant activity and can be used for development of functional beverages, nutritional health-care products and seasoning products.

To provide an endophyte involved in the dormancy state of plant seeds.SOLUTION: The present invention is directed to Pseudomonas sp. strains DB4, DB5, and DB9, and these bacterial strains have an effect of inhibiting the germination of plant seeds. These bacterial strains serve not only for inhibition of germination of plant seeds, but also as novel tools in research on dormancy of plant seeds.SELECTED DRAWING: None

The inventive technology relates to novel systems, methods, and strategies for the control of plant pathogens and herbivores. The inventive technology may specifically include novel compositions, systems, and methods configured to deliver inhibitory RNA molecules to a pathogen-infected plant. In a preferred embodiment, the invention may include genetically engineered endophytic bacteria configured to deliver one or more inhibitory RNA molecules to a pathogen-infected plant. One preferred embodiment may include a novel trans-kingdom delivery of hairpin RNA targeting viral encoded proteins resulting in the reduction of viral protein accumulation levels.

The invention belongs to the field of biological prevention and treatment, and particularly relates to a synthetic bacterial community of endophytic bacteria of ligusticum wallichii, application of the synthetic bacterial community, a plant protective agent for preventing and treating root rot and a method for cultivating disease-resistant seedlings. Wherein the synthetic bacterial community is composed of any multiple of microbacterium stutzeri, Rosella airborne and phytobacterium flavum. According to the method, the three endophytic bacteria Plantibacter flavus (P36-15), Microbacterium schleiferi (P48-28) and Rothia aeria (P48-75) which are separated and screened from rhizoma chuanxiong rhizomes are compounded in proportion and inoculated to rhizoma chuanxiong seedlings, so that root rot caused by fusarium oxysporum and fusarium solani can be prevented, the root rot resistance of rhizoma chuanxiong plants is improved, and the root rot resistance of rhizoma chuanxiong plants is improved. The field control effect and the medicinal material yield of the ligusticum wallichii for preventing and controlling the oxysporum type root rot and the eggplant rot type fusarium type root rot are improved, and the application and popularization value is achieved.

The invention relates to the technical field of microorganisms, and discloses a strain of Pantoea endophytic bacteria YNK-FB0056 and an application of the Pantoea endophytic bacteria YNK-FB0056. The collection number of the pantoea is CCTCC NO: M 2025644, and the pantoea has good capacity of sulfur oxidation, nitrogen fixation, phosphorus dissolving, zinc dissolving, siderophore production and high yield of indoleacetic acid, and can regulate the nutrient structure of soil and convert substances which are difficult to utilize by plants in the soil, thereby promoting the growth of the plants and improving the stress resistance of the plants. Besides, the sulfur-oxidizing bacterium YNK-FB0056 provided by the invention is a bacterium separated from oilseed rape farmland soil, has better adaptability when being applied to a planting environment compared with a strain which is subjected to mutagenesis screening in an extreme environment and a laboratory, and can be stably colonized in plant rhizosphere and soil, so that the growth promoting effect is stably exerted for a long time, and the sulfur-oxidizing bacterium YNK-FB0056 is safe and environment-friendly and has a wide application prospect. Wide application prospects are realized.