23 results about "Endophytic bacteria" patented technology

The invention relates to the technical field of agricultural microorganisms. The invention provides a compound microorganism bacterium suspension, a bacterium agent, a tobacco seed coating agent as well as a preparation method and application of the compound microorganism bacterium suspension. The compound microorganism bacterium suspension comprises a rice seed endophytic bacterium spiraceae R3 bacterium suspension and a rice seed endophytic bacterium stenotrophomonas R5 bacterium suspension; the mass ratio of R3 to R5 is 1: (0.5-1.5); the endophytic bacterium herbapiril R3 of the rice seeds is Herbapiril sp. R3, and the preservation number of the herbapiril R3 is CCTCC (China Center For Type Culture Collection) NO: M 20231757; the rice seed endophytic bacterium stenotrophomonas R5 is Shenotrophomonas sp. R5, and the preservation number of the rice seed endophytic bacterium stenotrophomonas R5 is CCTCC (China Center for Type Culture Collection) NO: M 20231758. The compound microbial suspension is used as a seed coating agent to coat tobacco seeds, and growth of tobacco root systems can be remarkably promoted.

The application discloses application of animal endosymbiont in assisting corals to resist heat stress. The application significantly improves the resistance of corals to high-temperature environment by applying the animal endosymbiont SCSIO 12664, and helps to protect the corals from the influence of heat stress. The application introduces the application of the animal endosymbiont SCSIO 12664, and the technical scheme aims to overcome the defects of the prior art, improve the resistance of corals to high-temperature heat stress, reduce the negative influence on the ecosystem, and achieve the goal of more sustainable and effective protection of corals.

The application discloses a broad-spectrum growth promoting bacterial agent combination for oil camellia and oil tea, a biological preparation and application thereof, and relates to the field of microbial technology.The endophytic bacteria are separated from healthy oil camellia plants, the abilities of the strains in dissolving phosphorus, fixing nitrogen, producing IAA, producing iron carrier and antagonizing the pathogenic bacteria of oil tea anthracnose are determined, the onion Burkholderia and Aspergillus niger with excellent comprehensive growth promoting functions are screened to prepare a combined bacterial agent, and the combined bacterial agent is verified to have a significant growth promoting effect on the oil camellia and the oil tea through a potting experiment.The combined bacterial agent promotes the growth, nutrient absorption and root development of the oil tea, significantly improves the enzyme activities related to the carbon, phosphorus and nitrogen cycles of the soil, and realizes the overall optimization of the "plant-soil" system.The application finds a broad-spectrum mixed bacterial agent suitable for the artificial planting of the oil camellia and the oil tea in a rocky desertification area, effectively improves the adaptability of the plants in the rocky desertification habitat, and provides a new microbial technical approach for the collaborative repair of multiple tree species in the area.

The application provides a camellia plant endophytic bacteria, a bacterial agent thereof and an anti-influenza virus A application; the camellia plant endophytic bacteria is Bacillus thuringiensis, which is preserved in the China General Microbiological Culture Collection Center with a preservation number of CGMCC No.32945. The fermentation product of the strain shows good antiviral activity in in vitro and in vivo experiments, can effectively inhibit the replication of influenza virus, and protect infected mice from lethal influenza virus; also shows the effect of regulating immune response, enhances the secretion of IFN-gamma by down-regulating the secretion of pro-inflammatory factors such as IL-6 and IL-10, thereby improving the immune resistance of the body.

This invention belongs to the field of microbial technology and discloses a synthetic microbial community for enhancing biological nitrogen fixation in karst grasslands, its construction method, and its application. The synthetic microbial community includes... Sinorhizobium americanum , Sionrhizobium sp. as well as Bacillus cereus Wherein: the Sinorhizobium americanum The accession number is GDMCC NO: 67796; Sionrhizobium sp. The accession number is GDMCC NO: 67797; Bacillus cereus The accession number is GDMCC NO: 67798. For leguminous forage grasses that require repeated sowing, the seeds are treated with a synthetic microbial community before being sown in the field; for perennial leguminous forage grasses that have already been planted, the synthetic microbial community is diluted with water and sprayed onto the surface of adjacent leguminous forage grasses. This invention screens out suitable and efficient root nodule endophytic bacteria in karst areas and constructs a synthetic microbial community to enhance the biological nitrogen fixation of leguminous forage grasses. It clarifies the technical details of the practical application of the synthetic microbial community in the field. The application of this technology can significantly improve the biological nitrogen fixation rate of leguminous forage grasses, significantly reduce the amount of nitrogen fertilizer used in forage grasses, and significantly increase forage grass yield.

The invention relates to the technical field of microorganisms, in particular to an atractylodes lancea endophytic bacterium ALX01 and application thereof, the atractylodes lancea endophytic bacterium ALX01 is named as Pseudomonas sp. ALX01, the preservation number is CCTCC NO: M20252198, the preservation place is China Center for Type Culture Collection, Wuhan University, Wuhan, China, on October 11, 2025, and the preservation number is CCTCC NO: M20252198. By utilizing the atractylodes lancea endophytic bacterium ALX01, the problem that high-content and high-stability production of aceratrol and beta-eudesmol in non-genuine production areas is difficult to realize in the prior art is solved.

This disclosure relates to biofertilizers. In particular, this disclosure relates to combinations and compositions of biofertilizers containing endophytic bacteria and mycorrhizae, as well as methods of using them.

This invention discloses a strain of Codonopsis pilosula endophytic fungus that produces extracellular polysaccharides and its applications. This Codonopsis pilosula endophytic fungus is a short-spore bacterium, named... Brevibacillus schisleri CpRG-1. The Codonopsis pilosula endophytic bacteria of the present invention exhibit excellent ability to produce extracellular polysaccharides, producing high concentrations of extracellular polysaccharides, which can greatly reduce the cost of extracting polysaccharides from the medicinal herb Codonopsis pilosula; at the same time, the extracellular polysaccharides produced by the Codonopsis pilosula endophytic bacteria not only have antibacterial and hypoglycemic activities, but also can promote the healing of diabetic foot ulcers and improve the intestinal microbiota, providing new ideas for the development of antibacterial drugs, diabetes prevention foods and drugs, and diabetic foot ulcer treatment foods and drugs.

The present application belongs to the field of biological control, and particularly relates to a synthetic bacterial community of Ligusticum chuanxiong Hara endophytic bacteria, uses thereof, a plant protection agent for preventing and treating root rot, and a method for cultivating disease-resistant seedlings. Plantibacter flavus (P36-15), Microbacterium schleiferi (P48-28), Rothia aeria (P48-75), are proportionally compounded, inoculated into Ligusticum chuanxiong Hara seedlings, can prevent root rot caused by Fusarium oxysporum and Fusarium solani, improve the ability of Ligusticum chuanxiong Hara plants to resist root rot, improve the field control effect and medicinal material yield for preventing and controlling Ligusticum chuanxiong Hara Fusarium oxysporum and Fusarium solani type root rot, and have popularization and application value.

The invention belongs to the field of biological prevention and treatment, and particularly relates to a synthetic bacterial community of endophytic bacteria of ligusticum wallichii, application of the synthetic bacterial community, a plant protective agent for preventing and treating root rot and a method for cultivating disease-resistant seedlings. Wherein the synthetic bacterial community is composed of any multiple of microbacterium stutzeri, Rosella airborne and phytobacterium flavum. According to the method, the three endophytic bacteria Plantibacter flavus (P36-15), Microbacterium schleiferi (P48-28) and Rothia aeria (P48-75) which are separated and screened from rhizoma chuanxiong rhizomes are compounded in proportion and inoculated to rhizoma chuanxiong seedlings, so that root rot caused by fusarium oxysporum and fusarium solani can be prevented, the root rot resistance of rhizoma chuanxiong plants is improved, and the root rot resistance of rhizoma chuanxiong plants is improved. The field control effect and the medicinal material yield of the ligusticum wallichii for preventing and controlling the oxysporum type root rot and the eggplant rot type fusarium type root rot are improved, and the application and popularization value is achieved.

The present application relates to the field of agricultural microorganism technology, and provides a compound microbial bacteria suspension, a bacterial agent, a tobacco seed coating agent, a preparation method and application thereof.The compound microbial bacteria suspension comprises rice seed endophytic bacteria Zooshunt R3 bacteria suspension and rice seed endophytic bacteria Stenotrophomonas R5 bacteria suspension; the mass ratio of R3 and R5 is 1:(0.5-1.5); the rice seed endophytic bacteria Zooshunt R3 is Herbaspirillum sp. R3, and the preservation number is CCTCC NO:M 20231757; and the rice seed endophytic bacteria Stenotrophomonas R5 is Stenotrophomonas sp. R5, and the preservation number is CCTCC NO:M 20231758.The present application uses the compound microbial bacteria suspension as a seed coating agent to coat tobacco seeds, which can significantly promote the growth of tobacco roots.

The invention belongs to the technical field of microorganisms, and particularly relates to an application of a P.megaterium H403 strain in improvement of saline-alkali soil and promotion of growth of saline-alkali soil plants. The prelisteria megatherium H403 is an endophytic bacterium which is separated and screened from kelp, has remarkable saline-alkali tolerance and growth promoting capacity, can normally grow and breed in an environment with the salinity being smaller than or equal to 5%, has a remarkable improvement effect on the physicochemical property of saline-alkali soil, can obviously improve the soil fertility, and has a good application prospect in the field of saline-alkali soil, saline-alkali soil, saline-alkali soil, saline-alkali soil, saline-alkali soil, saline-alkali soil and saline-alkali soil. The growth and development of saline-alkali soil plants can be obviously promoted, oxidative damage to the plants caused by saline-alkali stress can be relieved, and the saline-alkali tolerance of the plants is enhanced.

This invention provides a method for inducing rooting in Vaccinium tissue culture seedlings based on expanded clay granules, comprising the following steps: S1, preparing an aminated expanded clay granule-endophytic bacteria fermentation composite carrier; S2, preparing an expanded clay granule-gel composite substrate containing the aminated expanded clay granule-endophytic bacteria fermentation composite carrier; S3, after pretreatment of Vaccinium adventitious buds, aseptically inoculating them into the expanded clay granule-gel composite substrate, and culturing them at 22-24℃ and a light intensity of 1500-2000 Lx for 35-45 days to induce rooting; S4, after hardening off for 3-5 days, transplanting them into a mixed substrate of peat moss and perlite for acclimatization and cultivation for 15-20 days. This invention, using an aminated expanded clay granule-endophytic bacteria fermentation composite carrier and an expanded clay granule-gel composite substrate to induce rooting, can promote root primordia differentiation, stabilize the root growth microenvironment, standardize culture conditions to ensure stable rooting, and harden off for seedlings to enhance their stress resistance and adaptability, effectively improving the rooting quality and transplant survival rate of Vaccinium tissue culture seedlings, providing technical support for their efficient seedling cultivation.

The invention provides an induction method and application of tea tree leaf calluses. A method for inducing calluses of tea tree leaves comprises the following steps: selecting mature leaves of a tea tree, reserving petiole parts, cleaning for later use without disinfection, inoculating an obtained explant to an induction culture medium for induction culture, the induction culture medium being selected from any one of an MS culture medium, 2.5 mg / L of 6-BA and 0.1 mg / L of NAA; the MS culture medium is prepared from 1.5 mg / L of 6-BA, 0.5 mg / L of zeatin and 0.1 mg / L of IBA; the MS culture medium is prepared from 2.0 mg / L of zeatin and 0.1 mg / L of IBA. According to the method, it is found for the first time that cane sugar is a key cause of infectious microbe breeding in tea tree tissue culture, by removing cane sugar in the culture medium, the culture medium can be kept sterile for a long time even in an open environment (no bacteria grow for five months), the industrial problem of tea tree endophyte and infectious microbe pollution is successfully solved, and the pollution rate is almost zero.

The invention discloses a teak clone tissue culture and rapid propagation method and application, and belongs to the technical field of plant tissue culture. According to the method disclosed by the invention, teak segments are taken as explants, detoxification is carried out by utilizing alcohol, mercuric chloride, penicillin and streptomycin solutions, bacteria and endophytes on the surfaces of the explants can be effectively removed, and the blank of the existing detoxification method of the teak detoxified explants is filled; after tissue culture seedlings are obtained, a bud proliferation culture medium is used for expanding propagation, a large number of plants are obtained in a short time, a plant growth regulator is added into the culture medium to promote rooting of the tissue culture seedlings, a teak tissue culture seedling rooting culture medium formula is explored, rooting in a tissue culture bottle can be achieved, and the problem that the teak tissue culture seedlings are difficult to root all the time is solved; the later transplanting survival rate is obviously improved.

This invention belongs to the field of wastewater biological treatment technology, specifically a GR24-regulated triphasic algae-bacterial symbiotic and its construction method for treating pig farm wastewater. This invention uses *Chlorella vulgaris*-endophytic bacteria S395-2-*Polyspora spiralis* as the dominant bacterial species, inoculating them into a co-culture system at a certain cell number ratio, and adding 10... ‑9 The synthetic strigolactone analog GR24 was cultured under specific temperature, light intensity, and light-dark ratio conditions to construct a triphasic algal-bacterial symbiotic with uniform spherical diameter and strong stress resistance. This triphasic algal-bacterial symbiotic was used for the pretreatment of pig farm wastewater. This invention is simple to operate, low in cost, and can significantly improve the stability of the symbiotic in high-stress wastewater, providing reliable materials for subsequent antibiotic resistance gene (ARG) reduction, making it suitable for industrial-scale promotion.

The invention provides a growth-promoting composite endophytic bacterial agent and application thereof in phytoremediation of soil polluted by heavy metal cadmium. The growth-promoting composite endophytic bacterial agent is prepared from SYUECO-009 trophobacter pascens, SYUECO-010 pantoea ananas (Pantoea ananas) and SYUECO-008 pseudomonas sp., and the ratio of the SYUECO-009 trophobacter pascens to the SYUECO-010 pantoea ananas to the SYUECO-008 pseudomonas sp. To the SYUECO-009 trophobacter pascens to the SYUECO-010 pantoea ananas to the SYUECO-008 pseudomonas sp. Is (1 to 1.5) to (1 to 1.5) to (1 to 1.5). The three endophytic bacteria are all separated from cirsium setosum growing in heavy metal Cd polluted soil, SYUECO-009 is extracted from leaves, and SYUECO-010 and SYUECO-008 are extracted from roots. The fungicide can be applied in a seed soaking or root irrigation mode, the biomass, the cadmium enrichment amount and the soil nutrient content of cirsium setosum in cadmium-polluted soil can be remarkably improved, the remediation efficiency is high, and pollution is avoided.

This invention relates to the field of microbial inoculants, and particularly to a compound inoculant for controlling soil-borne diseases in farmland based on endophytic bacteria, and its preparation method. The compound inoculant is composed of a main inoculant, auxiliary inoculants, and a fungicide. The main inoculant includes *Talaromyces sp. strain HBUM07170*, *Rhizobium sp. NXC24*, and *Methylobacterium brachiatum*, while the auxiliary inoculants include *Mycobacterium sp.*, *Caulobacter sp.*, *Penicillium sp.*, and *Pyronema omphalodes*, with each strain having a concentration of not less than 10%. 8 The concentration of CFU / ml is 1:1 (main bacterial agent mixture to auxiliary bacterial agent mixture), and tebuconazole is used as the fungicide. During preparation, each bacterial strain is activated and cultured separately, then mixed according to the specified volume ratio before adding tebuconazole and stirring to dissolve. This invention's compound bacterial agent exhibits synergistic effects among the bacterial strains, effectively antagonizing Fusarium oxysporum, reducing the incidence and mortality of lily wilt, and promoting plant growth. It combines biological control with chemical-assisted antibacterial effects and can be used in the preparation of control formulations for soil-borne diseases in farmland and lily wilt.

The application discloses a method for inducing cluster buds and regenerated seedlings of Liriodendron tulipifera in vitro. Firstly, 3-4 cm plant materials containing terminal buds or axillary buds are cut from the tender branches of Liriodendron tulipifera, the endophytic bacteria in the plant is removed after disinfection, then the terminal buds or axillary buds are cut into 0.5-1 cm as explants, and then the explants are placed on a cluster bud induction medium for induction and culture for about 30 days, and then the induced cluster buds are cut and placed on an elongation medium for culture; when the rootless seedlings with a bud elongation of 2-3 cm are obtained, the seedlings are transferred to a rooting medium for rooting induction, and can be rooted after being cultured in the rooting medium for about 40 days. The terminal buds and axillary buds are used as the explants, the growth of the endophytic bacteria is effectively controlled, the cluster bud induction medium, the bud elongation medium and the rooting medium are screened, and the in-vitro regeneration system is established, thereby providing technical support for the propagation of Liriodendron tulipifera plants.

This invention discloses a method for synergistically enhancing phytoremediation of heavy metal-contaminated soil using nitrogen-sulfur-doped carbon quantum dots and endophytic bacteria. The method involves planting remediation plants in heavy metal-contaminated soil, applying nitrogen-sulfur-doped carbon quantum dots and endophytic bacteria for cultivation, and then completing the remediation process. This invention, by constructing a material-microorganism-plant synergistic remediation system, not only improves the absorption and translocation efficiency of heavy metals by remediation plants, overcoming the bottleneck of phytoremediation efficiency, but also enhances the resilience of remediation plants under heavy metal stress conditions. Simultaneously, it improves the rhizosphere microenvironment, promotes the enrichment of beneficial microorganisms, and thus helps maintain the long-term remediation effect of the soil. This method not only improves the efficiency of phytoremediation of heavy metal-contaminated soil but also enhances the adaptability of plants under heavy metal stress conditions, improves the rhizosphere microenvironment, and maintains soil microecological stability, demonstrating good application prospects and promotional value.

A system for evaluating the plant growth-promoting rhizobacterial properties (PGPR) of endophytic bacteria isolated from Acanthus ilicifolius L., and for assessing plant growth parameters, consisting of: a) an isolation unit for isolating and purifying endophytic bacteria from leaves of Acanthus ilicifolius L., the isolation unit comprising: • Surface sterilization device configured to treat leaf explants successively with Tween20 solution, 70% ethanol and 1% mercuric chloride, • Grinding device for homogenizing leaf tissue with phosphate buffer solution, • Incubation chamber configured for the cultivation of bacterial colonies on nutrient agar at 37°C; b) a characterization unit for the morphological, biochemical and molecular characterization of isolated bacteria, wherein the characterization unit comprises the following: • Equipment for Gram staining, • Equipment for biochemical tests for performing the indole test, the methyl red test, the citrate utilization test, the H2S production test, the gelatin hydrolysis test, the starch hydrolysis test and the catalase test, and • Molecular analysis equipment configured for 16S rRNA sequencing, including DNA isolation apparatus, PCR thermocycler and DNA sequencer; c) an optimization unit configured to analyze the culture conditions for maximum bacterial growth at different temperatures and pH values, the optimization unit comprising a colorimeter configured to measure absorbance at 610 nm to generate bacterial growth curves; d) a PGPR property evaluation unit for assessing the plant growth-promoting potential of isolated bacteria, wherein the PGPR property evaluation unit comprises the following: • Testing equipment for indole-3-acetic acid (IAA) production using the Salkowski color test, • Test apparatus for phosphate solubilization using the Pikovskaya medium • Devices for testing siderophore production using chromazurol S-agar (CAS agar) and • Test apparatus for the activity of 1-amino-1-cyclopropane-1-carboxylate (ACC) deaminase using Dworkin and Foster minimal salt medium; and e) a plant growth evaluation unit configured for in vitro analysis of the effects of bacterial inoculum on plant growth parameters such as stem length, root length, leaf length and chlorophyll content.

The application discloses a kind of composite microbial inoculant with the function of promoting growth and degrading thiamethoxam and application, the composite functional inoculant is composed of 2 thiamethoxam-degrading bacteria Enterobacter cloacae and Enterobacter cloacae and 1 growth-promoting enterobacter, and is all plant endophytic bacteria, the composite inoculant can be applied to soil source thiamethoxam pollution leaf vegetable system, can effectively reduce thiamethoxam residue in leaf vegetable while promoting plant growth, realizes green and safe production.

The invention discloses endophytic bacteria for tissue culture seedlings of flintley and application of the endophytic bacteria, and relates to the technical field of microorganisms. The endophytic bacterium (Bacillus sterilis) OdE-III of the tissue culture seedling of the flintley is preserved in the China Center for Type Culture Collection on September 1, 2025, and the preservation number of the endophytic bacterium is CCTCC (China Center for Type Culture Collection) NO: M 20251913. The flintleaf flintleaf herb endophytic bacterium OdE-III provided by the invention is derived from a flintleaf flintleaf herb tissue culture seedling plant stem, can inhibit the growth of pathogenic bacteria in soil and improve the growth vigor of the flintleaf flintleaf herb tissue culture seedling after transplanting, breaks through the technical bottleneck that the high-quality flintleaf flintleaf herb is dead of illness and poor in growth after transplanting, and is beneficial to promoting the artificial planting of the flintleaf flintleaf herb.