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Complex endophytic bacterial inoculant and application thereof in phytoremediation of heavy metal contaminated soil

A technology of endophytic bacteria and heavy metals, applied in the field of phytoremediation, can solve the problems of small biomass, slow growth, weak adaptability, etc., and achieve the effect of enhancing absorption capacity, promoting growth and improving efficiency

Active Publication Date: 2016-09-21
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although Sedum sedum has some advantages in terms of growth rate and biomass compared with most heavy metal hyperaccumulator plants, its efficiency in remediating soil heavy metal pollution is still limited by slow growth, small biomass, weak adaptability and the availability of heavy metals in the soil. Therefore, how to promote the growth of Sedum sedum, stress resistance, increase biomass and the ability to absorb, transport and accumulate heavy metals in soil is of great significance for the practical application of Sedum sedum to repair heavy metal-contaminated soil

Method used

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  • Complex endophytic bacterial inoculant and application thereof in phytoremediation of heavy metal contaminated soil
  • Complex endophytic bacterial inoculant and application thereof in phytoremediation of heavy metal contaminated soil
  • Complex endophytic bacterial inoculant and application thereof in phytoremediation of heavy metal contaminated soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 promotes the overall characteristics of the composite endophytic bacterial agent for Sedum sedum bioremediation

[0050] Inoculate 200 μL of the prepared bacterial agent into 100 mL medium, incubate at 35°C for 36 hours, and a part of the obtained bacterial agent is used for preservation. Add 300 μL sterilized glycerol and 700 μL logarithmic phase bacterial solution to a 1.5 mL sterile centrifuge tube. Store at -80°C (can be stored for 2 years), and the remaining liquid bacterial agents are identified for key physiological and biochemical indicators.

[0051] 1. Identification of pathogenicity

[0052] Inoculate the above-mentioned bacterial solution to fresh damaged leaves (Sedum sedum, tobacco, rapeseed), use sterile water as a control, and no leaf disease characteristics are found; extract 100 μL of bacterial solution with a sterile syringe and inject it into fresh and healthy onion stems , using sterile water as a control, cultured at 30°C for one week, n...

Embodiment 2

[0071] Example 2 Effect of Endophytic Bacteria Compound Bacteria on the Growth of Sedum Sedum and Heavy Metal Soil Remediation

[0072] The composition of the nutrient solution is: K 2 SO 4 , 0.7mmol / L; KCl, 0.1mmol / L; Ca(NO 3 ) 2 4H 2O, 2mmol / L; MgSO 4 ·7H 2 O, 0.5mmol / L; KH 2 PO 4 , 0.1mmol / L; H 3 BO 3 , 10μmol / L; MnSO 4 ·H 2 O, 0.5 μmol / L; ZnSO 4 ·7H 2 O, 1μmol / L; (NH 4 ) 4 MoO 24 4H2O, 0.01μmol / L; FeSO 4 ·7H 2 O dissolved in boiling 20 μmol / L Na 2 ·In EDTA solution, 20μmol / L. Adjust the nutrient (pH 5.8) with NaOH or HCl

[0073] (1) Hydroponic environment

[0074] Rapid propagation of Sedum sedum by hydroponics. Cut off the newly born branches of Sedum sedum, each about 10cm long, leaving only 5-8 leaves at the top of the branches, insert them into 0.1 times the hydroponic nutrient solution, the temperature is 23-25°C, and the light conditions are 200-200°C. 300μE / m 2 s, after 3 weeks, the branches grow a little root, then culture with 1 times of t...

Embodiment 4

[0085] Embodiment 4 promotes the application of Sedum sedum bioremediation endophytic bacteria

[0086] Rapid propagation of Sedum sedum seedlings (method with reference to embodiment 3): get Sedum sedum top branch and take root in nutrient solution for about 45 days. LB liquid medium cultured PLSa01 bacterial agent overnight, washed the medium with sterile water, and pressed 10 5 CFU were inoculated into rooting seedling baskets, and cultured with aeration for 1 week. At this time, the endophytic bacteria of Sedum sedatus will colonize inside the root and form a symbiotic relationship with the root system.

[0087] Cutting propagation: 1 week after Sedum inoculated with endophytic bacteria, the endophytic bacteria will colonize the inside of the roots, plant Sedum sedum seedlings in heavy metal compound polluted mine soil, the seedling density is 70~ 100 plants. It grows slowly when it is first planted, and it is the growing season from September to December and March to J...

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Abstract

The invention discloses a complex endophytic bacterial inoculant and an application thereof in phytoremediation of heavy metal contaminated soil. The complex inoculant is composed of acinetobactercalcoaceticus, pseudomonas fluorescens, bacillusmegaterium, stenotrophomonasmaltophilia, bacillus pumilus and pseudomonas putida. The complex inoculant disclosed by the invention, when applied to heavy metal pollution remediation, integrates advantages of various endophytic bacteria and overcomes disadvantages of phytoremediation; and the complex inoculant can promote the growth of sedum alfredii hance, enhance the absorbing capacity of the sedum alfredii hance to heavy metals and improve the efficiency of phytoremediation.

Description

technical field [0001] The invention relates to the technical field of phytoremediation, in particular to a composite endophytic bacterial agent and its application in phytoremediation. Background technique [0002] With the rapid development of industry, the modernization of agricultural production and the rapid advancement of urbanization, soil heavy metal pollution is becoming more and more serious. [0003] Heavy metals cannot be degraded by microorganisms, and have the characteristics of poor mobility, long residence time, concealment, and irreversibility. Once soil is polluted by heavy metals, it is extremely difficult to reduce them. On the one hand, heavy metals in the soil can affect various metabolic activities such as plant growth and cell division, inhibit the growth of crops and reduce crop yields. On the other hand, heavy metals in soil can enter the food chain through the soil-crop system, posing a huge risk to human health. In the face of dozens of mass inc...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/01C12R1/39C12R1/11C12R1/07C12R1/40
Inventor 陈宝徐晓萌潘风山冯英吴飞飞杨肖娥
Owner ZHEJIANG UNIV
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