61 results about "Sedum alfredii" patented technology

The invention relates to a technique of remediation of slightly and medio- metal-contaminated vegetable soil with production. The specific steps are as follows: firstly, Sedum alfredii and Elsholtzia splendens are intercropped in vegetable soil contaminated by heavy metal such as cadmium, copper and zinc in spring with a with inter plant distance of ten to fifteen centimeters, row spacing of thirty centimeters; secondly, the Sedum alfredii is harvested periodically, a growth period of Sedum alfredii is three to four months during a first harvest, from then on, the Sedum alfredii is harvested every three months or when growing to a height of thirty to forty centimeters, stems of a height of two to three centimeters are left above the ground in harvest; the Elsholtzia splendens is harvested in October; thirdly, a cadmium-enriched rape is planted after the harvest of the Elsholtzia splendens with the spacing in the row of fifteen to twenty centimeters and is harvested in April of the next year; fourthly, a cucumber is planted after the harvest of the rape with the spacing in the row of twenty to twenty-five centimeters, a cabbage is planted after the harvest of the cucumber in September, the Elsholtzia splendens is planted after the harvest of the cabbage, then a restoring period is completed. Adopting a super accumulator plant for absorbing a plurality of heavy metal from the soil, the invention achieves reducing the heavy metal content in the vegetables and guaranteeing the safety of the farm produce.

The invention relates to a plant system repairing method for composite polluted soil of chromium, zinc, lead and copper. According to the invention, Sedum alfredii H and Elsholtzia harchowensis are intercropped in the composite polluted soil of chromium, zinc, lead and copper, the root system of the Sedum alfredii H absorbs chromium, zinc and lead, the Elsholtzia harchowensis absorbs copper, and the Sedum alfredii H and the Elsholtzia harchowensis transport the pollutions to the overground part, the overground part is harvested and removed regularly or according to the growth height of the Sedum alfredii H and the Elsholtzia harchowensis, thereby removing a plurality of chromium, zinc, lead and copper from the soil, the biological normal position repair for the composite polluted soil of chromium, zinc, lead and copper can be implemented. The method is economic and convenient, and has no secondary pollution, and the invention can conserve both moisture and soil, and can beautify the environment.

The invention relates to an ecological restoration method for a coal gangue dump; according to the method, during the arrangement of a coal gangue dump and the arrangement of the dump body, annular plant ditches and longitudinal drainage ditches are disposed from top to bottom; clay is added onto gangue and is tamped; a mixture of dehydrated sludge and gangue is added onto the clay between ditches, and sedum alfredii hance are planted; a mixed substrate of gangue dump surface soil, fermented pig manure, and vermiculite is added into the plant ditches; and prostrate spurge, crape myrtle, and cucurbita pepo are planted orderly and repeatedly in the mixed substrate. The method effectively reduces the spontaneous combustion probability of coal gangue, shortens the restoration period, makes use of dehydrated activated sludge, changes wastes into valuables, and obtain certain economic benefits, environmental benefits, and social benefits.

The invention discloses a method for repairing soil pollution caused by heavy metals by the combining technologies of interacting plants and chemical leaching, which realizes combining repairing by the interacting between Sedum alfredii Hance and corns as well as by carrying out chemical leaching by a mixing additive. The interacting plants selected by the invention are the corns and the Sedum alfredii Hance and are planted on the soil polluted by the heavy metals; when the Sedum alfredii Hance and the corns enter a growing blooming period, the mixing additive is used for carrying out leaching on the soil to reduce the heavy metals in the soil by harvesting plants and leaching liquid, thereby realizing the repairing on the soil polluted by the heavy metals. The content of the heavy metals in the corn seeds produced by the invention meets the national sanitation standard for feedstuff, the content of the heavy metals at the stem and leaf parts of the corn plants meets the standard of commercial organic fertilizer, and the corns are farm produces with economic values. The leaching waste liquid produced by the invention can be fixed and processed by lime. The method of the invention can realize that agricultural manufacture is carried out on the polluted soil while repairing and treatment. The method of the invention is a new effective repairing approach for soil polluted by the heavy metals with low cost.

The invention discloses a complex endophytic bacterial inoculant and an application thereof in phytoremediation of heavy metal contaminated soil. The complex inoculant is composed of acinetobactercalcoaceticus, pseudomonas fluorescens, bacillusmegaterium, stenotrophomonasmaltophilia, bacillus pumilus and pseudomonas putida. The complex inoculant disclosed by the invention, when applied to heavy metal pollution remediation, integrates advantages of various endophytic bacteria and overcomes disadvantages of phytoremediation; and the complex inoculant can promote the growth of sedum alfredii hance, enhance the absorbing capacity of the sedum alfredii hance to heavy metals and improve the efficiency of phytoremediation.

The invention discloses a sedum alfredii endophyte and an application thereof. The endophyte is named as Acinetobacter calcoaceticus Sasm3 with a preservation number of CGMCC NO.7334. The invention also discloses an application of the endophyte in plant-microorganism combined remediation on heavy metal contaminated soil. The application comprises the steps of: inoculating sedum alfredii with the endophyte and planting the sedum alfredii inoculated with the endophyte in the heavy metal contaminated soil to be remedied; and from the second growing season of the sedum alfredii inoculated with the endophyte, re-inoculating the sedum alfredii with the endophyte in each growing season, and harvesting the sedum alfredii. The endophyte can be used for enhancing the resistance of the sedum alfredii to high-concentration heavy metal so as to promote the healthy growth of the sedum alfredii in the environment of high-concentration heavy metal, thereby increasing the biomass of the sedum alfredii and enhancing the remediation efficiency of the sedum alfredii to the heavy metal contaminated soil.

The invention discloses a sexual reproducing method of southeast Crassulaceae, which comprises the following steps: harvesting ripe seed of Crassulaceae; exposing; picking; sieving; separating; airing under shadow; reserving at -20-0 deg. c; freezing the seed below 4 deg. c for 2-3 weeks before germinating; using tap water to select seed; sterilizing the seed through 0. 5%KMnO4; germinating on the paper bed; harvesting the seeding; transmitting the seeding into the base with vermiculite and pearlite; culturing through full-nourishing liquid of dicotyledon; transplanting the seeding into field until the seeding is 3-4cm. The invention can harvest large amount of sexual reproduced sprout of southeast Crassulaceae, which can restore the plant in the heavy metal polluted environment.

The invention relates to a method of accelerating accumulation of heavy metals cadmium and zinc by sedum alfredii hance. The method comprises the following steps: 1) planting sedum alfredii hance on soil polluted by heavy metals cadmium and zinc; when the sedum alfredii hance grows to 80-90 days, adding plant hormones and a chelating agent into the soil; and moving the integral plant from the soil in 10 days for harmless treatment; and 2) detecting concentration of heavy metals cadmium and zinc in the soil; if the concentration does not meet the environmental safety standard, then planting the next batch of sedum alfredii hance; and repeating the process of the step 1) till the concentration of heavy metals cadmium and zinc in the soil meets the environmental safety standard. According to the method provided by the invention, by using the degradable chelating agent GLDA and applying growth hormones in a matched manner, the repair efficiency of soil polluted by heavy metals cadmium and zinc by the plant sedum alfredii hance is effectively improved, and secondary pollution on the environment is not caused. The method has the characteristics of low cost, high operability, safety and the like, and has good social, economical and environmental benefits.

The invention discloses a phytoremediation activator and a phytoremediation method for heavy metal contaminated soil. In terms of 1000 kg of the phytoremediation activator, the phytoremediation activator is prepared from the following components: 30-350 mol of GLDA (N,N-bis(carboxymethyl)-L-glutamic acid tetrasodium salt), 100-200 kg of ground phosphate rock, 250-450 kg of sawdust, 50-200 kg of auxiliary materials and the balance of water. GLDA, the ground phosphate rock and the sawdust are combined, the obtained phytoremediation activator can significantly improve heavy metal arsenic and cadmium extraction capacity of Sedum alfredii and octopus, and soil remediation efficiency is improved.

The invention discloses a method for improving phytoremediation efficiency of farmland soil with light cadmium pollution by using potash fertilizer, which comprises the following steps in sequence: (1) transplanting seedlings of a plant with super cadmium accumulation, particularly referring to the seedlings of sedum alfredii hance, in soil with the light cadmium pollution and fertilizing 2.3-2.5Kg of pure potassium in a form of sulfate fertilizer per mu; and (2) harvesting overground parts of the sedum alfredii hance when the overground parts of the sedum alfredii hance are 50-60cm high. after used for repairing the farmland soil with the light cadmium pollution, the method disclosed by the invention has the advantages of being high-efficiency, economical, green, environment-friendly and the like.

The invention discloses a gene capable of promoting cadmium accumulation and application of the gene. The gene capable of promoting cadmium accumulation is separated from super accumulation type sedum alfredii hance, the polynucleotide sequence is shown by (a) or (b), (a) is a polynucleotide sequence shown by SEQ ID No.1; (b) is a polynucleotide sequence which is complementary with the polynucleotide sequence in the (a) according to a base complementary pairing rule. The gene is derived from a super accumulation type sedum alfredii hance plant, an expression vector of the gene is constructed, agrobacterium is converted with an expression vector electric shock method, arabidopsis thaliana is soaked with a mature agrobacterium transformation method, and finally, a transgenic arabidopsis thaliana plant is obtained. The content of cadmium in the blade of the transgenic arabidopsis thaliana plant can be remarkably increased by over expression of the gene on the blade of the transgenic arabidopsis thaliana plant, compared with an ordinary arabidopsis thaliana plant, the content of cadmium in the transgenic arabidopsis thaliana subjected to cadmium treatment is improved by 50%-64%, and the absorption rate of the cadmium in the transgenic arabidopsis thaliana plant under heavy metal cadmium stress is remarkably higher than that of the cadmium in the ordinary arabidopsis thaliana plant.

The invention discloses a method for repairing contaminated soil in a coal-to-gas plant by adopting a microbe-plant combined way. The method comprises the following steps: adding an electronic receptor preparation in the contaminated soil so as to promote anaerobic degradation of polycyclic aromatic hydrocarbons and analysis of heavy metals; mixing and composting the contaminated soil with pig manure and a conditioning agent for several days; eluting partial heavy metals from the soil by using clear water; planting sedum alfredii hance and festuca arundinacea in the soil in a mixed way so as to extract the heavy metals from the soil and promote microbial degradation of the polycyclic aromatic hydrocarbons in the soil, wherein in the growth stage of plants, additives are sprayed periodically to enhance the repairing effect. Through the adoption of the method, the environment friendliness is very high, the repairing cost is low, and the ecological environment of the contaminated soil can be effectively restored, so that the method has a broad market prospect.

The invention discloses a method for repairing arsenic-cadmium-lead composite contaminated soil by oxalic acid reinforced ciliate desert-grass-sedum alfredii hance intercropping, and the method comprises the following steps: ploughing a land to be remediated, and adding a base fertilizer; uniformly transplanting ciliate desert-grass and sedum alfredii hance seedlings which are consistent in size into the arsenic-cadmium-lead composite contaminated soil, ensuring proper temperature for planting, and arranging a nylon net between two rows of plants to separate root systems; 15-20 days before harvesting, applying an oxalic acid aqueous solution to plant root soil; and regularly mowing the above-ground parts of the ciliate desert-grass and the sedum alfredii hance. According to the method, oxalic acid is adopted to reinforce the ciliate desert-grass-sedum alfredii hance intercropping to restore the arsenic-cadmium-lead composite contaminated soil, and unique planting measures are adopted for synergistic effect, so that the removal effect of plants on arsenic, cadmium, lead and other heavy metals in the soil is remarkably improved.

The invention discloses a method for repairing heavy metal soil through sedum alfredii hance with the assistance of an alternating current electric field. The method comprises the steps that sedum alfredii hance plants are selected, an organic material serves as a base fertilizer, and the sedum alfredii hance plants are transplanted to the to-be-repaired soil; the organic material comprises, by weight, 100 parts of peat soil, 50-75 parts of soymeal slag, 15-20 parts of coconut coir, 10-15 parts of carbonized rice hulls and 12-16 parts of lees; and an alternating current is applied into the to-be-repaired soil, the voltage strength is 0.4-1.1 V/cm, the energizing time lasts for 15-20 days, and energizing is conducted for 5-8 h every day. According to the method for repairing the heavy metal soil through the sedum alfredii hance with the assistance of the alternating current electric field, the alternating current specific in voltage strength and energizing time is applied into the heavy metal soil and is matched with the specific organic material to assist the sedum alfredii hance plants in repairing the heavy metal soil, the migration efficiency of heavy metal in the soil can be significantly improved, and the amount of the heavy metal accumulated through the over-ground part of the plant can be significantly increased, so that the repairing efficiency of the heavy metal soil is improved.

The invention discloses a method for repairing a cadmium-polluted rice field by intercropping sedum alfredii hance and astragalus sinicus. The sedum alfredii hance and the astragalus sinicus are planted in an idle rice field in winter, so that effective utilization of land resources and photo-thermal resources is realized, and idle land in the slack winter season and resource waste are avoided while soil of the low-concentration cadmium-polluted rice field is repaired; and the astragalus sinicus can be used as organic green manure; and the sedum alfredii hance and the astragalus sinicus are intercropped, so that cadmium in the soil is effectively removed, and therefore, post-cropping rice absorbs less cadmium.

The invention discloses a method for extracting zinc from farmland soil continuously by utilizing hyper-accumulator plants, which comprises the following steps of: applying 50 to 800 milligrams of soluble phosphate fertilizer to each kilogram of zinc-polluted soil, and applying 0.2 gram of nitrogen and 0.2 gram of potassium which serve as base fertilizers; pre-culturing stems with axillary buds of sedum alfredii hance by using nutrient solution for 3 to 4 weeks, transplanting seedlings of the sedum alfredii hance of which the root system is eugenic and the plant height is between 6 and 8 centimeters in the treated zinc-polluted soil, managing growth according to the conventional farming method, harvesting overground parts of the sedum alfredii hance every four months, and reserving 2 to 3 centimeters of the stems of the overground parts during harvesting; and harvesting the overground parts of the sedum alfredii hance at regular intervals to reduce the concentration of the zinc in the soil continuously. The method is easy and convenient to operate, the physicochemical property of the soil can be improved effectively, and the repair efficiency of the zinc-polluted soil can be improved; and the method has the advantages of small investment, small work amount, simple management technical requirement, wide application range and the like, and soil environment is not damaged.

The invention discloses a method for repairing cadmium-polluted farmland while conducting production, belonging to the technical field of environmental management of polluted soil. The method comprises the following steps: (1) intercropping sedum alfredii hance and Lingxiyicun broad beans on to-be-remediated soil; (2) spraying a cadmium activating agent to leaves of the broad beans and the sedum alfredii hance in the seedling stage and the flowering stage of the broad beans, and completely removing straws of the broad beans and the sedum alfredii hance after harvesting; (3) leveling the soil,applying a cadmium passivation conditioner, and uniformly mixing the cadmium passivation conditioner with plough layer soil; (4) after one week, transplanting rice Zhongzheyou No.8 , carrying out flooding treatment in the whole growth period, spraying a selenium-enhanced cadmium blocking and controlling agent on leaf surfaces in the tillering period and the booting period of the rice, draining water one week before harvesting, and completely removing straws after harvesting the rice; and (5) repeating the steps (1)-(4) until soil safety reaches the standard. According to the method, the sedumalfredii hance and the broad beans are intercropped and then are subjected to crop rotation with the rice, and various agricultural and strengthening measures are cooperatively conducted, so remediation of cadmium-polluted soil is completed while safe production of crops is guaranteed.

The invention relates to a method to plant on the fertilizer from solid waste. It uses the sludge from city sewage farm. The plant is Alocasia marorrhiza or Alocasia marorrhiza and Sedum alfredii H. Potassium in haulm of alocasia marorrhiza reaches to 10wt%, N, P₂O₅, K₂O reaches to 15wt%. It is a high content natural compounding organic potassium fertilizer.

The invention discloses a method for repairing a cadmium-polluted rice field by intercropping sedum alfredii and broad beans. The sedum alfredii and the broad beans are planted on an idle rice field in winter, so that effective utilization of land resources and photo-thermal resources is realized, and the winter-fallowed land and wasting of resources are avoided while soil of the rice field polluted by low-concentration cadmium is repaired; the cadmium content of fruits meets the national food safety limit standard under the condition that the yield of the broad beans is slightly reduced; themethod has important significance for ensuring the food safety of China and the health of people; and the cadmium is passivated to a certain extent, so that the cadmium is absorbed less by rice whichis used as a later crop.

The invention discloses a composite seed soaking agent and application thereof in improving the crop seed vigor and reducing the zinc content of seedlings under zinc stress. The composite seed soakingagent comprises a component A and a component B according to a volume ratio of 1: (1-100), wherein the component A is prepared by mixing 1 to 100 mM of salicylic acid, 1 to 150 mM of indole acetic acid and 10 to 200 mM of citric acid by taking water as a solvent; the component B is a sedum alfredii hance extract and is obtained by air-drying and crushing sedum alfredii hance plants and then putting crushed sedum alfredii hance plants into water for extraction. Salicylic acid, indole acetic acid and citric acid are used as effective components of the seed soaking agent; the compound seed soaking agent with a remarkable synergistic effect is prepared by combining the effective components with the sedum alfredii hance extract that can enrich zinc ions, and after the compound seed soaking agent is used for soaking crop seeds at a proper concentration, the accumulated zinc in crop seedlings can be effectively reduced, and the seed vigor and seedling quality of crops are improved.

The invention relates to a seed treatment method for accelerating sedum alfredii hance seed germination, which comprises the steps of seed selection, low-temperature variable magnetic field germination acceleration, high-temperature hormone germination acceleration, normal-temperature strong magnetic field germination acceleration, cleaning and disinfection, and sowing. Seed selection, low-temperature variable magnetic field germination acceleration, high-temperature hormone germination acceleration, normal-temperature strong magnetic field germination acceleration, cleaning and disinfection are sequentially carried out on sedum alfredii hance seeds stored at normal temperature, then seedling raising or planting is carried out, the germination rate of the sedum alfredii hance treated through the method can reach about 90% 28 days after sowing, the germination rate of the sedum alfredii hance can reach about 95% 35 days after sowing. The dormancy time of the sedum alfredii hance seeds stored at normal temperature can be effectively and greatly shortened, the germination rate is greatly increased, the seedling rate of the sedum alfredii hance seeds can be effectively increased, and the production period of the sedum alfredii hance can be remarkably shortened, so that the breeding cost of the sedum alfredii hance is reduced, the breeding efficiency of the sedum alfredii hance is improved, and the increasingly serious remediation requirements of heavy metal contaminated soil in China are met.

The invention relates to the field of heavy metal polluted plant extraction, and aims to provide a method for increasing the plant extraction efficiency by means of carbon dioxide enrichment and endophyte interaction. The method comprises the following steps: preincubating a hyperaccumulator namely sedum alfredii hance with tap water, selecting seedlings which are 1 to 2cm in root lengths, soakingthe root systems into an endophyte composite bacteria solution for inoculating, and then transplanting the seedlings into a black plastic bucket, wherein a nutrient solution in the black plastic bucket contains cadmium; adding the endophyte composite bacteria solution, placing the seedlings into a plant growth chamber for culturing, keeping carbon dioxide in the plant growth chamber being enriched, changing the nutrient solution once every three days, and keeping the cell concentration; culturing under a carbon dioxide enrichment condition for 1 month, and harvesting plants. By adopting the method, the photosynthesis and biomass are enhanced by a carbon dioxide enrichment technology; meanwhile, the endophyte is applied to promote the adsorption and accumulation of heavy metal cadmium by hyper accumulators, the defects of slow plant growth and small biomass in a plant extracting technology are overcome, the environmental adaptability of the hyperaccumulator sedum alfredii hance is enhanced, and the plant extracting efficiency is increased remarkably.

The invention discloses an interplanting technique with the function of improving orchard soil properties. The interplanting technique comprises the following steps: soil around peach roots is loosened in March-April, in terms of a soil loosening range, a circle is formed from the peach roots by extending outwards for 40-50 cm, and fine sand is scattered to the loosened soil; humus fertilizer is applied to the loosened soil according to 1-2 kg/m<3>, then watering is performed according to 0.01-0.05 m<3>/m<2>, the soil is aired under natural conditions for 3-5 days, and the fertilizer permeatesinto the soil around the peach roots slowly; 1-year-old seedlings of sedum alfredii are transplanted around the peach roots, and the loosened soil around the peach roots is watered every day for three continuous days after transplanting according to 0.01-0.05m<3>/m<2>; residual land of a peach orchard is loosened at the depth of 5-10 cm, diameter of soil particles is kept at 1-5 cm, watering is performed after soil loosening according to 0.02-0.05 m<3>/m<2>, and then seeds of paspalum notatum, trifolium repens and arachis pintoi are scattered. With adoption of the interplanting technique, content of heavy metal cadmium in the soil can be reduced remarkably, and surface soil loss amount can be reduced.

The invention discloses a hydroponic floating seedling method for sedum alfredii hance. The method comprises the following steps of (1) collecting upper branches of annular sedum alfredii hance free of diseases and pests, cutting the upper branches into cuttings, and standing in a cool and ventilated place for wound healing; (2) soaking lower parts of the healed cuttings into a growth-promoting liquid and cutting on a perforated foam board, floating the foam board into clear water, and making the lower parts of the cuttings in contact with the clear water and the upper parts in contact with air, wherein the growth-promoting liquid is an auxin solution or a plant growth-promoting rhizobacteria solution; and (3) replacing the clear water regularly during the seedling period and transplanting after the roots grow to 5cm. The method is free of limitation of the season, highly intensive, multiple in seedlings per unit area, fast in emergence and good in industrialization prospect, industrial large-scale seedling is achieved, and a lot of seedlings can be quickly provided for soil heavy metal restoration within a short period; the seedlings are relatively good in root development, relatively healthy and strong, free of weed and free of soil-borne diseases and pests and relatively high in transplanting survival rate; a seedbed is saved; and manpower and material resources are saved.