14 results about "Trifolium repens" patented technology

The invention provides a soil mixing and sowing method of typical herbaceous plants and a method for forming a biological ridge of a newly-built terrace edge ridge, which comprises the following steps of: 1) selecting qualified trifolium repens, ryegrass, alfalfa and vicia villosa seeds according to process requirements; 2) seed pretreatment: soaking trifolium repens and alfalfa seeds with warm water at 30-40 DEG C for 2-4 hours; the method comprises the following steps: soaking ryegrass and vetch seeds in cold water for 1-2 hours; (3) respectively and uniformly mixing the four seeds with soil according to the proportion required by the process; (4) uniformly sowing the seed-soil mixture on a target land parcel; and 5) management after sowing. According to the herbaceous vegetation soil mixing and sowing method, high germination and survival rate of the herbaceous vegetation can be guaranteed, the plant height, the above-ground biomass and the vegetation coverage of all the herbaceous vegetation are continuously increased in the growing season, and the root system biomass is in the trend of firstly decreasing and then increasing; and all herbaceous vegetation improves the water retention performance of the soil.

The invention discloses a traditional Chinese medicine composition for breaking diseases, removing food retention and removing stasis, a preparation and a preparation method of the traditional Chinese medicine composition. The traditional Chinese medicine composition for breaking diseases, removing food retention and removing stasis is prepared from the following traditional Chinese medicinal materials in parts by weight: 12-18 parts of ardisia japonica, 5-9 parts of rhizoma dioscoreae nipponicae, 8-14 parts of berba aristolochiae mollissimae, 3-7 parts of radix araliae armatae, 7-11 parts of radix tetrastigme, 6-10 parts of herba cistanche and 3-7 parts of lithospermum erythrorhizon. The traditional Chinese medicine compound disclosed by the invention is rigorous in compatibility, takes both symptoms and root causes into consideration, takes effects of breaking diseases, removing food retention, removing blood stasis and dredging collaterals as the core, also takes effects of dispelling wind, eliminating dampness, clearing away heat and toxic materials, tonifying kidney, strengthening bones and supporting healthy energy into consideration, can quickly relieve pain symptoms, can fundamentally improve the core pathogenesis of wind-cold damp evil invasion, qi stagnation, blood stasis and meridian obstruction, and reduces the recurrence rate.

This invention discloses a novel structural polysaccharide SYQE3-F derived from the roots of *Tripterygium wilfordii*, its preparation method, pharmaceutical composition, and applications, belonging to the field of biomedical technology. This invention is the first to isolate and purify a homogeneous polysaccharide SYQE3-F from the roots of *Tripterygium wilfordii*, with an average molecular weight of 13.174 kDa. Its main chain consists of →4)-β-D-GlcpA-(1→) and →2)-α-D-Manp-(1→), and its side chains include t-Galp and t-Araf. This invention also discloses a pharmaceutical composition containing this polysaccharide and vitamin E. In vitro and in vivo experiments show that this polysaccharide can effectively break down choroidal occlusion barriers and significantly promote the transport of vitamin E to the target retinal tissue, providing a novel natural drug combination for the clinical treatment of DR.

The application provides a cold-resistant gene TrWRKY10a540, an expression vector, application and breeding method thereof, and belongs to the field of plant molecular genetics and genetic improvement. Specifically disclosed is a cold-resistant gene TrWRKY10a540 and an expression vector derived from Trifolium repens L. and application thereof in improving the cold resistance of plants, and the problem of insufficient research on low-temperature stress related genes of Trifolium repens in the prior art is solved. The application further discloses a breeding method for regulating the cold stress resistance of plants by the cold-resistant gene TrWRKY10a540, and research results show that the gene can significantly improve the cold resistance and antioxidant level of plants under low-temperature stress conditions, and has good functional effects. The application can be widely applied to plant molecular breeding, is particularly suitable for improving the low-temperature resistance of economic crops such as forage grasses, provides a new genetic resource for the breeding of cold-resistant plants, and has important significance for revealing the regulation mechanism of the cold resistance of plants.

The invention provides a traditional Chinese medicine composition and a preparation method thereof, and belongs to the technical field of traditional Chinese veterinary medicines, and the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: spring eyes, cynanchum otophyllum, moonwort, gordon euryale seed and the like. The traditional Chinese medicine composition belongs to a pure traditional Chinese medicine preparation, has the effects of calming liver and reducing blood pressure, clearing heat and eliminating phlegm, and tonifying kidney and spleen, and has the advantages of remarkable treatment effect, high blood pressure reducing speed, no toxic or side effect and the like on dog hypertension diseases. The traditional Chinese medicine composition provides a new theoretical data basis and thought for treating the dog hypertension, and has wide market potential in treating the pet dog hypertension diseases.

The application of Trifolium repens TrWRKY7c1 gene in improving plant cold resistance belongs to the technical field of plant genetic engineering and crop genetic breeding. In order to solve the technical blank of the existing technology about the research on low-temperature stress related genes of Trifolium repens, the application of Trifolium repens TrWRKY7c1 gene in improving plant cold resistance is provided, the nucleotide sequence of the gene is shown as SEO ID NO. 1, and the amino acid sequence encoded by the gene is shown as SEO ID NO. 2; a recombinant cloning vector, a plant expression vector or a recombinant glycerol preservation strain containing the gene are also provided, and a transgenic plant is obtained through an agrobacterium-mediated genetic transformation method. The TrWRKY7c1 gene provided in the application can be applied to improve plant cold resistance, cultivate cold-resistant plants, and provide a new gene resource for plant cold resistance breeding, and has important significance for the improvement of cold resistance and the research on the regulation mechanism of plant growth and development.

This invention discloses a method for improving the fruit set rate of Hovenia dulcis, belonging to the field of agricultural production technology. Addressing the low fruit set rate caused by anther abortion, insufficient pollinating insects, and self-incompatibility, this invention comprehensively applies three synergistic measures: First, spraying a hormone solution of GA 35 μg / g and IAA 3 μg / g during the bud stage promotes pollen and style development; second, attracting native bees by planting motherwort and white clover, and leasing and introducing Italian bee colonies during peak flowering to enhance natural pollination; finally, preparing and spraying an artificial pollination solution containing specific concentrations of sucrose, boric acid, and calcium ions during the flowering period for assisted pollination. This method systematically solves the bottlenecks in each stage of pollination, increasing the fruit set rate of Hovenia dulcis from a natural 12.67% to a maximum of 71.24%, resulting in a significant yield increase and wide applicability to the development of the Hovenia dulcis industry.

The application provides a kind of cluster root fungus agent for improving the cold resistance of durian and a preparation method thereof, and belongs to the technical field of microbial inoculant.The preparation method of the cluster root fungus agent comprises the following steps: collecting subfrigid zone plant rhizosphere soil, and screening cluster root fungus spores in the soil;inoculating the cluster root fungus spores into sand mixed culture medium, then planting white clover or ryegrass and other plants to carry out enrichment culture and obtain culture;mixing the culture with cutting substrate, and cutting one-year-old durian branches to carry out rooting culture and first low-temperature culture;storing the root system of cutting seedlings in cold storage, crushing, mixing with cutting substrate, cutting one-year-old durian branches to carry out second low-temperature culture, and collecting the root system of cutting seedlings and rhizosphere soil to obtain the cluster root fungus agent.The cluster root fungus agent can induce durian to produce low-temperature resistant protective enzymes, thereby improving the cold resistance of durian and providing a new idea for cultivating durian cold-resistant varieties.

This invention belongs to the field of traditional Chinese medicine application technology and provides an application of a traditional Chinese medicine composition in the treatment of radiation-induced pneumonia. The traditional Chinese medicine composition is made from bamboo sap and *Trifolium repens*; the mass ratio of bamboo sap to *Trifolium repens* is (1-10):(1-10). Radiation-induced pneumonia refers to lung inflammation caused by radiotherapy for malignant tumors of the chest. The traditional Chinese medicine composition provided by this invention has the effects of clearing heat and detoxifying, resolving phlegm and dissipating nodules. It has a simple composition and high safety, providing a new option for the treatment of radiation-induced pneumonia.

This application discloses a hair thickening and anti-hair loss composition for improving thinning hair and its preparation method, belonging to the field of daily chemical product technology. The composition includes active ingredients: Polygonum multiflorum root extract, ginger root extract, Ligustrum lucidum fruit extract, Cordyceps militaris extract, Ligusticum chuanxiong root extract, Centella asiatica extract, Rosa rugosa fruit extract, adenosine, biotin tripeptide-1, and Trifolium repens extract; and excipients: solubilizer, penetration enhancer, humectant, preservative, and deionized water. This application achieves its hair thickening and anti-hair loss efficacy through a dual mechanism of action combining traditional Chinese medicine meridian strengthening and modern target activation. Experiments have shown that the composition can significantly increase the ratio of hair growth phase to resting phase, increasing hair follicle density by more than 35%, and skin irritation tests have confirmed no irritation or toxic side effects. This application also provides a preparation method for the composition, which is simple, mild, and suitable for industrial production.

The invention provides a cocksfoot and associated seed mixed sowing method for weed prevention and control and application of the cocksfoot and associated seed mixed sowing method for weed prevention and control, and belongs to the technical field of ecological planting of forage grass and green prevention and control of weeds. And weed germination and growth are inhibited by utilizing interspecific competitive advantages and root allelopathy of dactylis glomerata. According to the method, under the condition that little or no chemical herbicide is applied, the dactylis glomerata can keep community advantages for a long time, the weed biomass and population density are continuously reduced, ecological, safe, long-acting and stable weed prevention and control are achieved, and the method is suitable for artificial grassland planting, degraded grassland improvement and green feed production and has ecological benefits and production benefits.

This invention relates to the field of animal husbandry technology, specifically to a fermented compound herbal feed additive for lambs, its preparation method, and its application. The fermented compound herbal feed additive for lambs, by weight, comprises the following components: 10-20 parts of *Trifolium repens*, 10-20 parts of raw malt, 5-15 parts of *Astragalus membranaceus*, 5-15 parts of *Saposhnikovia divaricata*, 5-15 parts of *Medicated Leaven*, 5-15 parts of dried tangerine peel, 5-15 parts of green tangerine peel, 5-15 parts of *Dryopteris crassirhizoma*, 3-5 parts of stir-fried *Atractylodes macrocephala*, and 3-5 parts of calcined oyster shell. When added to the feed of pre-weaning lambs, the fermented herbal ingredients of this invention improve lamb growth performance, promote growth hormone secretion, enhance immunity, and alleviate lamb stress.

The invention provides a nitrogen-fixing paenibacillus sp. SY6, a microbial preparation containing the nitrogen-fixing paenibacillus sp. SY6 and application of the nitrogen-fixing paenibacillus sp. SY6, and belongs to the technical field of agricultural microorganisms and environmental microorganisms. The preservation number of the bacillus SY6 is CGMCC (China General Microbiological Culture Collection Center) No. 35901. The paenibacillus SY6 disclosed by the invention is derived from rhizosphere of Trifolium repens L., and the nitrogenase activity of the paenibacillus SY6 is obviously higher than that of common combined nitrogen-fixing bacteria. Whole genome analysis shows that the paenibacillus SY6 has a complete molybdenum-dependent (nif) and vanadium-dependent (vnf) double nitrogenase system at the same time, and has the capability of synthesizing indoleacetic acid (IAA) through an indole-3-pyruvic acid (IPyA) pathway. The paenibacillus SY6 can be effectively colonized at the rhizosphere of non-host crops such as corn and the like, can promote the elongation of plant root systems, nitrogen absorption and biomass accumulation, and is suitable for farmland nitrogen management, nitrogen fertilizer reduction, soil ecological restoration and microbial fertilizer development.

This invention belongs to the field of plant cultivation technology, specifically relating to a method for treating seeds of *Trifolium repens* and a method for raising seedlings of *Trifolium repens*. The treatment method includes (1) screening *Trifolium repens* seeds and refrigerating them; (2) washing the seeds in water at 40-60℃; then soaking the seeds in a gibberellin solution and drying them. This method, through refrigeration, washing the seeds at 40-60℃, and soaking them in a gibberellin solution, can improve the germination rate and the seedling rate in the later seedling stage. After treating *Trifolium repens* seeds using this method, germination can begin in 40 days, reaching its peak at 60 days. When the treated seeds are sown, the average germination rate per pot is 95%. Compared with the conventional seedling raising germination rate (below 15%) and germination time (120 days) of existing technologies, this invention significantly improves the germination rate and shortens the germination time. Furthermore, the treated seeds, when sown, have a higher seedling rate.