Cultivation method of trifolium repens

A cultivation method and white clover technology, applied in the field of white clover cultivation, can solve the problems of poor drought resistance, large water demand, rare addition combination, etc., and achieve the effects of alleviating growth retardation and improving drought resistance.

Active Publication Date: 2017-09-15
SICHUAN AGRI UNIV
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Problems solved by technology

However, at present, most of the research reports on exogenous addition to improve plant stress resistance use a single additive, while there are few reports on improving the drought resistance of white clover through exogenous addition at home and abroad. Effective addition combinations especially rare
[0005] Therefore, in view of the poor drought resistance of white clover in production and cultivation, the demand for water is large, the loss caused by drought stres...

Method used

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  • Cultivation method of trifolium repens
  • Cultivation method of trifolium repens
  • Cultivation method of trifolium repens

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Experimental program
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Embodiment

[0050] Test material: Drought-sensitive 'Latinuo' white clover was used as the test material.

[0051] Material cultivation: The seeds are sterilized with 0.1% mercuric chloride for 5 minutes, rinsed with distilled water for 4 times and then used for later use. 0.1g of the sterilized seeds are evenly sprinkled in a seedling tray covered with quartz sand with a length of 35cm, a width of 25cm, and a height of 10cm, pour deionized water and place the seedling tray in an incubator (23 / 19°C, day / night ;75% relative humidity; 300μmol·m -2 ·s -1 The light intensity) allows the seeds to germinate for a period of 7 days, and the deionized water is changed every day. After the seeds have germinated, pour out all the deionized water and replace it with Hoagland's nutrient solution for cultivation, replace the nutrient solution every other day, and continue to cultivate for 23 days (2 mature leaves) to select materials with consistent growth for the test .

[0052] Experimental desig...

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Abstract

The invention discloses a cultivation method of trifolium repens which can substantially increase the drought resistance of trifolium repens. The cultivation method of trifolium repens comprises following steps: disinfecting selected trifolium repens seeds; spraying the seeds in a seedling growing plate and adding deionized water to the seedling growing plate; placing the plate in an incubator for 5 to 9 days for germination, after germination is finished, the deionized water in the seedling growing plate is replaced with Hoagland's nutrient solution, and continuing placing the plate in the incubator for 21 to 25 days for seedling growing; after the seedling growing is finished, the trifolium repens grows seedlings and then applying exogenous additive to the roots of the seedlings; the exogenous additive is made of Hoagland's nutrient solution, 0.5g/L-1.5g/L CTS and 50.58mg/L-151.74 mg/L Spm. By means of the exogenous additive made of the drought resistance composition, the growth inhibition and leaf wilting of trifolium repens under the drought stress can be effectively alleviated; compared with single dose, the exogenous additive has substantial synergy effect and the drought resistance of trifolium repens can be substantially increased. The method is suitable for application in forage cultivation field.

Description

technical field [0001] The invention relates to the field of pasture cultivation, in particular to a method for cultivating white clover. Background technique [0002] White clover (Trifolium repens) is a perennial leguminous forage widely cultivated in the world. It has high grass yield and good quality. All kinds of livestock like to eat it. It can provide high-quality forage and fix soil nitrogen in grassland. double effect. In addition, white clover is also an important soil and water conservation and garden greening plant in environmental protection and ecological construction. However, the phenomenon of yield reduction or death caused by drought is becoming more and more prominent in production. Therefore, in the cultivation of white clover, it is urgent to explore cultivation measures and methods to improve the drought resistance of white clover. [0003] Drought stress is one of the main abiotic stress factors affecting plant growth and yield. Due to global clima...

Claims

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Application Information

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IPC IPC(8): A01G31/00A01C1/08
CPCA01C1/08A01G31/00
Inventor 彭燕张艳李亚萍李州张新全马啸黄琳凯闫艳红
Owner SICHUAN AGRI UNIV
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