The invention discloses a preparation method for a
mulberry leaf healthy food substrate. The method comprises the steps of a, pulverization: leaves of
mulberry trees,
Moraceae, Morus are taken as theraw material, the surfaces of the leaves are cleaned, and
mulberry leaf powder is obtained after the leaves are dried and pulverized; b, pre-enzymolysis: water and
cellulase are added to the mulberryleaves according to a certain proportion, and pre-enzymolysis is conducted; c,
ultrasound-assisted enzymatic method extraction: certain volume of water and complex
enzyme are added again, ultrasonic wave
irradiation is exerted, and a
mulberry leaf crude extract A is obtained; d, refining: the mulberry leaf crude extract A passes through an
ultrafiltration membrane with the molecular weight of 10000 Da, and permeating liquid is collected; the permeating liquid passes through an
ultrafiltration membrane with the molecular weight of 500 Da to collect intercepted fluid, and the substrate is obtained after vacuum concentration and atomizing
drying are conducted on the intercepted fluid. The primary components of the prepared food substrate are flavone and n-saccharide compounds, enzymatic pre-treatment and
ultrasound-assisted enzymatic method extraction technologies are adopted to greatly improve the extraction rates of mulberry leaf flavone and
alkaloid, and the method has the advantages of being simple and convenient in technology, green efficient and significant in effect.