Cloning method and application of mulberry anthocyanin-3-O-glucoside-2-O-glucuronic acid transferase gene
A technology of glucuronic acid and glucoside, applied in the biological field, can solve the problems of uncloned and applied research reports, few biosynthetic pathways and mechanisms of mulberry anthocyanins, and achieve the effect of promoting quality improvement
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[0053]The technical solutions of the present invention will be further described in detail below in conjunction with specific embodiments.
[0054] 1.1 Materials and main reagents
[0055] The mulberry variety tested was Yuechenda 10 (preserved in the mulberry germplasm resource nursery of Sericulture Research Institute, Sichuan Academy of Agricultural Sciences), and the fruit (mulberry) of Yuechenda 10 was purple-black at maturity. The plant total RNA rapid extraction kit (B518631-0100), AMV first-strand cDNA synthesis kit (B532445-0020) and reverse transcription kit (R223) used were purchased from Sangon Bioengineering (Shanghai) Co., Ltd. The vector pMD19-T, DNA polymerase, and real-time fluorescence quantitative PCR reagents were purchased from Bao Biological Engineering (Dalian) Co., Ltd., primers were synthesized by Sangon Bioengineering (Shanghai) Co., Ltd., and Escherichia coli competent cells were prepared by the laboratory. The rest of the reagents were analytically...
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