Cloning method and application of mulberry anthocyanin-3-O-glucoside-2-O-glucuronic acid transferase gene

A technology of glucuronic acid and glucoside, applied in the biological field, can solve the problems of uncloned and applied research reports, few biosynthetic pathways and mechanisms of mulberry anthocyanins, and achieve the effect of promoting quality improvement

Inactive Publication Date: 2021-03-16
SICHUAN ACAD OF AGRI SCI SERICULTURE INST
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Due to the high content of mulberry anthocyanins, the development and utilization of mulberry anthocyanins is becoming a hot spot, but there are still few studies on the biosynthetic pathway and mechanism of mulberry anthocyanins
However, there is no report on the cloning and application of the mulberry anthocyanin-3-O-glucoside-2-O-glucuronosyltransferase (MaUGAT) gene

Method used

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  • Cloning method and application of mulberry anthocyanin-3-O-glucoside-2-O-glucuronic acid transferase gene
  • Cloning method and application of mulberry anthocyanin-3-O-glucoside-2-O-glucuronic acid transferase gene
  • Cloning method and application of mulberry anthocyanin-3-O-glucoside-2-O-glucuronic acid transferase gene

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Embodiment Construction

[0053]The technical solutions of the present invention will be further described in detail below in conjunction with specific embodiments.

[0054] 1.1 Materials and main reagents

[0055] The mulberry variety tested was Yuechenda 10 (preserved in the mulberry germplasm resource nursery of Sericulture Research Institute, Sichuan Academy of Agricultural Sciences), and the fruit (mulberry) of Yuechenda 10 was purple-black at maturity. The plant total RNA rapid extraction kit (B518631-0100), AMV first-strand cDNA synthesis kit (B532445-0020) and reverse transcription kit (R223) used were purchased from Sangon Bioengineering (Shanghai) Co., Ltd. The vector pMD19-T, DNA polymerase, and real-time fluorescence quantitative PCR reagents were purchased from Bao Biological Engineering (Dalian) Co., Ltd., primers were synthesized by Sangon Bioengineering (Shanghai) Co., Ltd., and Escherichia coli competent cells were prepared by the laboratory. The rest of the reagents were analytically...

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Abstract

The invention discloses a cloning method and application of a mulberry anthocyanin-3-O-glucoside-2-O-glucuronic acid transferase gene. The cloning method comprises the following steps: extracting total RNA of a mulberry test sample and synthesizing a cDNA first chain; cloning a mulberry MaUGAT gene. The invention further discloses application of the mulberry anthocyanin-3-O-glucoside-2-O-glucuronic acid transferase gene cloned by the method in a mulberry germplasm cultivation process. One anthocyanin-3-O-glucoside-2-O-glucuronic acid transferase gene is compared and identified from a Sichuan mulberry genome database, and the gene is cloned from Yueshengda 10 mulberries by designing a specific primer. The method enriches related data in the mulberries and even moraceae plants, provides candidate genes for genetic improvement of the mulberries, promotes improvement of the quality of the mulberries, and better serves industrialization of fruit mulberries.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for cloning anthocyanin-3-O-glucoside-2-O-glucuronosyltransferase gene of mulberry tree and application thereof. Background technique [0002] Anthocyanin is one of the important pigments that determine the color of plant flowers, fruits and seeds, and belongs to flavonoid polyphenols. In recent years, a large number of studies have found that anthocyanins have various biological activities such as anti-inflammation, anti-tumor, and blood lipid regulation, indicating that anthocyanins can play an important role in plant stress resistance and prevention of human chronic diseases. Due to the high content of mulberry anthocyanins, the development and utilization of mulberry anthocyanins is becoming a hotspot. However, there are few studies on the biosynthetic pathway and mechanism of mulberry anthocyanins. Anthocyanin biosynthesis is part of the flavonoid biosynthesis pathway, w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10C12N15/54C12N15/82A01H5/00A01H6/00
CPCC12N9/1051C12N15/825C12Y204/01254
Inventor 曾益春黄盖群刘刚危玲代洁郑继川姚永权李永远
Owner SICHUAN ACAD OF AGRI SCI SERICULTURE INST
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