2 results about "Rutaceae" patented technology

This invention discloses a butterfly adult directional attractant based on volatile chemical substances, along with its preparation and application methods. The invention uses a combination of readily available chemical reagents, such as linalool, as volatile signaling substances to efficiently induce various butterfly adults, including the Common Mormon and the Citrus Swallowtail, which use citrus plants (Rutaceae family) as hosts, to complete forward directional movements through flight or walking. This method can induce the synchronized directional movements of multiple butterflies of various species at a relatively low cost, and has significant practical value for human-butterfly interaction projects in ecological landscape construction and recreational experience settings.

The application discloses a living extraction method of citrus Huanglongbing bacteria, relates to the technical field of plant pathogenic bacteria extraction, and solves the problem that the prior art cannot guarantee that the citrus Huanglongbing bacteria have complete biological activity and high purity at the same time. The method comprises four core steps of pretreatment of Rutaceae plant samples, extraction of bacterial liquid, initial enrichment of the bacterial liquid and re-enrichment of the bacterial liquid in sequence, the whole extraction process is carried out in a low-temperature environment, plant tissues infected with pathogenic bacteria are broken by freezing and grinding, and step-by-step extraction and enrichment of the citrus Huanglongbing bacteria are realized by combining step-by-step differential centrifugation and sucrose density gradient centrifugation. The method can effectively retain the complete biological activity of the citrus Huanglongbing bacteria, significantly improve the purity of the bacterial liquid, and the prepared bacterial liquid can be used for infection of healthy Rutaceae plants and microinjection transfection of citrus psyllids, the method is simple in operation and good in repeatability, and provides stable and reliable experimental materials for the research on the pathogenic mechanism, transmission rule and prevention and control technology of the citrus Huanglongbing disease.