11 results about "Rutaceae" patented technology

The invention relates to the technical field of extraction, in particular to a method for improving rutaceous characteristic aroma of pericarpium citri reticulatae, which comprises the following steps: firstly extracting with purified water, then carrying out hot extraction with fragrant raw wine, and then mixing and centrifugally separating, coarsely filtering with a ceramic membrane and finely filtering with an ultrafiltration membrane. The fragrance has rutaceous characteristic fragrance, and is still clear and transparent after 30 days of stability observation. The preparation process is simple, is suitable for industrial production, can be used for flavoring cocktail and liqueur, and increases the aroma components of the wine body.

This invention discloses a butterfly adult directional attractant based on volatile chemical substances, along with its preparation and application methods. The invention uses a combination of readily available chemical reagents, such as linalool, as volatile signaling substances to efficiently induce various butterfly adults, including the Common Mormon and the Citrus Swallowtail, which use citrus plants (Rutaceae family) as hosts, to complete forward directional movements through flight or walking. This method can induce the synchronized directional movements of multiple butterflies of various species at a relatively low cost, and has significant practical value for human-butterfly interaction projects in ecological landscape construction and recreational experience settings.

The application discloses a living extraction method of citrus Huanglongbing bacteria, relates to the technical field of plant pathogenic bacteria extraction, and solves the problem that the prior art cannot guarantee that the citrus Huanglongbing bacteria have complete biological activity and high purity at the same time. The method comprises four core steps of pretreatment of Rutaceae plant samples, extraction of bacterial liquid, initial enrichment of the bacterial liquid and re-enrichment of the bacterial liquid in sequence, the whole extraction process is carried out in a low-temperature environment, plant tissues infected with pathogenic bacteria are broken by freezing and grinding, and step-by-step extraction and enrichment of the citrus Huanglongbing bacteria are realized by combining step-by-step differential centrifugation and sucrose density gradient centrifugation. The method can effectively retain the complete biological activity of the citrus Huanglongbing bacteria, significantly improve the purity of the bacterial liquid, and the prepared bacterial liquid can be used for infection of healthy Rutaceae plants and microinjection transfection of citrus psyllids, the method is simple in operation and good in repeatability, and provides stable and reliable experimental materials for the research on the pathogenic mechanism, transmission rule and prevention and control technology of the citrus Huanglongbing disease.

The invention relates to the technical field of functional food, in particular to a medicinal and edible stale flavor biscuit for treating ovarian reserve function decline and application thereof, and the medicinal and edible stale flavor biscuit comprises the following raw materials in parts by weight: 20-25 parts of traditional Chinese medicine mixed powder, 25-30 parts of low-gluten flour, 7-9 parts of butter, 11-14 parts of powdered sugar, 0.6-1.0 part of baking soda and 14-18 parts of egg yolk. The traditional Chinese medicine mixed powder is composed of pericarpium citri reticulatae powder, Chinese yam powder and poria cocos powder according to the ratio of 1: 1: 1, the pericarpium citri reticulatae powder is prepared by crushing dried mature peels of rutaceous plant oranges at low temperature, the Chinese yam powder is prepared by crushing dried iron stick yam tubers, and the poria cocos powder is prepared by crushing dried poria cocos tubers. The raw materials of the product are safe and mild and can synergistically condition the body; the process is optimized, the palatability is improved, and satiety and convenience are achieved; the method has quality control guarantee, fills the market blank, provides reference for functional food development, and is suitable for long-term eating of target people.

The invention provides a solid biological bacterial fertilizer for rutaceae. The solid biological bacterial fertilizer is prepared by inducing probiotic expression of rutaceae plants, culturing and screening specific flora, and developing a bacterium-charcoal-plant triple interaction carrier based on pomace resource utilization: squeezing and pyrolyzing a pomace-rice hull mixture to prepare a charcoal carrier, and activating flora induction by flavanol; the composite bacterial liquid is wrapped by pectin-activated clay gel to form a soil response type slow release structure, and the solid biological bacterial fertilizer capable of improving rutaceous soil, the yield of agricultural products and the quality of fruits is obtained.

To provide a surface treatment composition excellent in durability of fragrance from a hard surface when attached to the hard surface of an article.SOLUTION: A surface treatment composition used for an article having a hard surface, the surface treatment composition comprising 0.1 to 20% by mass of a betaine-based amphoteric surfactant as a component (a); An essential oil containing α - pinene extracted from at least one plant selected from the group consisting of Labiatae, Pinaceae, Cupressaceae, Myrtaceae and Rutaceae, and 0.01 to 1% by mass of a polysaccharide as a component (c), wherein a content of the component (b) in the composition for surface treatment is such an amount that a content of the α - pinene in the composition for surface treatment is 0.001 to 1% by mass, A mass ratio calculated by a formula of [content of α - pinene (% by mass) / content of component (c) (% by mass)] / content of component (a) (% by mass) is 0.01 to 10.SELECTED DRAWING: None

The invention discloses a method for overcoming distant hybridization incompatibility of rutaceous plants by using AS-sjgRK3 and application of the method. According to the method, an antisense oligonucleotide technology is adopted, and an AS-sjgRK3 reagent is dropwise added to stigmas of rutaceous plants, so that the incompatibility of distant hybridization of the four-season oranges and the clausena lansium is overcome. The method is small in damage to plants, remarkable in effect of overcoming the distant hybridization incompatibility and wide in application range, can be further used for overcoming the distant hybridization incompatibility of other rutaceous plants, and has important value for breeding and seed production of the rutaceous plants.

The present invention discloses an artificial large-scale breeding equipment and breeding method for swallowtail butterflies in the Yangtze River Delta region, the breeding method comprising: (1) selecting a geographical location and arranging a net house; (2) selecting, arranging, and maintaining swallowtail butterflies, host plants, and nectar plants; (3) breeding swallowtail butterflies; and (4) transferring adults. The breeding equipment is constructed in an area meeting the above environmental conditions, wherein the Yangtze River Delta region, an environment with a natural temperature of 5-35°C and a relative humidity of 55%-80%, are selected. The present invention controls economic costs and artificially breeds a variety of native butterflies such as citrus swallowtail butterflies, jade-banded swallowtail butterflies, and jade-spotted swallowtail butterflies, which use citrus plants as host plants. The invention can achieve successful introduction of all insect stages from eggs to adults and ensure generational breeding, with a high breeding survival rate and less manual operation. The invention can solve the problem that the current local butterfly breeding technology in the Yangtze River Delta region is insufficient to support ecotourism and the ornament processing industry, and can also avoid the invasion of alien species and protect local butterfly germplasm resources.

The invention relates to a corydaline 9-O-methyltransferase PaOMT1, a coding gene of the corydaline 9-O-methyltransferase PaOMT1 and a function of the corydaline 9-O-methyltransferase PaOMT1, wherein the corydaline 9-O-methyltransferase PaOMT1 is used for catalyzing (S)-corydaline in Phellodendron amurense of rutaceae to form (S)-tetrahydrocotetrandrine. Belongs to the technical field of gene engineering. The invention discloses an open reading frame sequence of amur corktree PaOMT1 gene and an amino acid sequence coded by the amur corktree PaOMT1 gene. Candidate OMT genes are screened on the basis of amur corktree genome, transcriptome and metabolome data, and functions of the amur corktree OMT genes are verified in vitro by constructing a pET28a-OMTs prokaryotic expression vector and taking (S)-corydaline as a substrate. The result proves that the PaOMT1 has the function of catalyzing the (S)-golden corydalis alkali to form the (S)-tetrahydrocolumbamine, in addition, the PaOMT1 can further catalyze the (S)-tetrahydrocolumbamine to form the (S)-tetrahydropalmatine, and a foundation is laid for the biological research on synthesis of the protoberberine alkaloid with important medicinal value.

The invention discloses a method for overcoming distant hybridization incompatibility of rutaceous plants by using AS-sjgFER1 and application of the method. According to the method, an antisense oligonucleotide technology is adopted, and an AS-sjgFER1 reagent is dropwise added to stigmas of rutaceous plants, so that the incompatibility of distant hybridization of the four-season oranges and the clausena lansium is overcome. The method is small in damage to plants, remarkable in effect of overcoming the distant hybridization incompatibility and wide in application range, can be further used for overcoming the distant hybridization incompatibility of other rutaceous plants, and has important value for breeding and seed production of the rutaceous plants.

The application provides a preparation method of high-content naringin and hesperidin extract, and utilizes a microwave-assisted eutectic solvent optimization process to prepare naringin and hesperidin in pericarpium citri reticulatae, and the extraction conditions of the microwave-assisted eutectic solvent are optimized through single-factor experiments and BBD (Box-Behnken Design) response surface experiments, and finally the optimal extraction conditions are obtained as follows: the molar ratio of choline chloride and ethylene glycol is 1:6, the water content is 10%, the solid-liquid ratio is 10 mg / mL, the microwave extraction temperature is 94 DEG C, the time is 41 min, and the power is 400 W. The eutectic solvent is used as the extraction solvent, and the microwave-assisted extraction technology with high energy transmission efficiency and easy to be applied in large scale is combined, so that the efficiency can be improved in future industrial application, the extraction process is more environment-friendly and sustainable, and a new idea is provided for deep processing and utilization of Rutaceae plant resources.