89 results about "Cucurbitaceae" patented technology

The invention discloses a balsam pear extract and preparation thereof. The extract liquid is made from extraction using fruit of cucurbit balsam pear as raw material. The extraction process includes using water as the solvent, and extracting the balsam pear extract liquid by zymohydrolysis, ultrasonic processing or the like. The balsam pear extract liquid is in light yellow or yellow, contains active substance such as balsam pear saponins, balsam pear polypeptides and active polysaccharide. The process is simple and fast, easy for industrial production, with nutritious extract, without environment pollution, and having low cost. The balsam pear extract has evident function of blood glucose and blood lipid lowering.

The invention discloses a full-automatic cucurbitaceae garden stuff grafting device which comprises a stander, a parental stock conveying mechanism, a grafted seedling conveying mechanism and a cion conveying mechanism that are arranged on the stander in parallel from left to right; a parental stock clip straight-line motion mechanism is transversely arranged on the parental stock conveying mechanism and the grafted seedling conveying mechanism and a cion clip straight-line motion mechanism is transversely arranged on the grafted seedling conveying mechanism and the cion conveying mechanism; a parental stock aperture disk is arranged on an operation position of the parental stock conveying mechanism and a parental stock root pruning mechanism, a parental stock growing point cutting mechanism and a parental stock clamping mechanism which is arranged on the parental stock clip straight-line motion mechanism in a sliding way are arranged above the parental stock aperture disk; a grafted seedling aperture disk is arranged on an operation position of the grafted seedling conveying mechanism and a parental stock perforating mechanism and a stroma perforating mechanism are arranged above the grafted seedling aperture disk; a cion aperture disk is arranged on an operation position of the cion conveying mechanism and a cion cutting and supporting mechanism and a cion clamping mechanism which is arranged on the cion clip straight-line motion mechanism in a sliding way is arranged above the cion aperture disk.

The invention relates to crop cultivation technology, in particular to high yield cultivation technology for vegetables, which is suitable for cultivating vegetables such as Solanaceae (tomatoes, eggplants, hot pepper and the like) and Cucurbitaceae (cucumbers, pumpkins, hornedmelon and the like), and belongs to the technical field of crop cultivation. The technology comprises the steps of seed breeding, cultivation management, extermination of disease and insect pest and the like; from the seed breeding, through a series of ordered management technology, seeds are grown to the vegetables which become safe foods on the dining table of people; and the economic benefit of vegetable growers is effectively improved.

Methods of prophylaxis or treatment of a disease state initiated by or characterized by platelet aggregation, that employ a fruit extract or active fraction thereof, are disclosed. In one embodiment, the fruit extract or active fraction thereof, is obtained from the fruit of plants of the families Solanaceae, Rutaceae, Cucurbitaceae, Rosaceae, Musaceae, Anacardiaceae, Bromeliaceae, Vitaceae, Arecaceae, Ericaceae and Lauraceae. Pharmaceutical compositions comprising a fruit extract or active fraction thereof having platelet aggregation inhibitory activity are also disclosed.

The invention provides a green tea beverage for reinforcing immunity, reducing blood fat, losing weight and clearing away summer heat, comprising the following main components in parts by weight: 3 to30 parts of fiveleaf gynostemma herb, 1 to 10 parts of lotus leaves and 1 parts of green tea. The fiveleaf gynostemma herb is leaves of curcurbitaceae sweet gynostemma pentaphylla, grows in west areas of Hubei Province, and specially contains trace element selenium. The green tea beverage is used for solving susceptible fatigue and other diseases caused by low immunity, can simultaneously and effectively regulate blood fat and lose weight if the beverage is drunk for long time, has simple manufacture process, less equipment investment and low product cost, and is applicable to industrial andspecialized production in large scale.

Disclosed is an infectious clone vector of a melon necrotic spot virus. The infectious clone vector pCB301-MNSV is obtained by recombination of a melon necrotic spot virus whole genome and a plant expression vector pCB301-2x35S-MCS-HDVRZ-NOS with a 35S promoter and ribozyme Rz. The infectious clone vector pCB301-MNSV is transferred into agrobacterium GV3101 competent cells through a freeze-thaw method, and then a melon seedling is inoculated to detect infectivity of the pCB301-MNSV through an agrobacterium injection method. The infectious clone vector is expressed in agrobacterium strongly, is capable of infecting cucurbit plants such as melons, watermelons and cucumbers but cannot infect tobacco, animals and humans, and accordingly relative safety is achieved and studies on viruses can be operated on the DNA (deoxyribonucleic acid) level easily because of infectious cloning success.

Methods of prophylaxis or treatment of a disease state initiated by or characterized by platelet aggregation, that employ a fruit extract or active fraction thereof, are disclosed. In one embodiment, the fruit extract or active fraction thereof, is obtained from the fruit of plants of the families Solanaceae, Rutaceae, Cucurbitaceae, Rosaceae, Musaceae, Anacardiaceae, Bromeliaceae, Vitaceae, Arecaceae, Ericaceae and Lauraceae. Pharmaceutical compositions comprising a fruit extract or active fraction thereof having platelet aggregation inhibitory activity are also disclosed.

The present disclosure relates to methods of producing a sweet juice composition from monk fruit and other fruit of the Cucurbitaceae family containing mogroside V and other terpene glycosides. The methods employ a cation exchange resin and an anion exchange resin to obtain a sweet juice composition with a clean flavor. The sweet juice composition can be used in a food, beverage, pharmaceutical or dietary supplement product.

The present disclosure relates to methods of producing a sweet juice composition from monk fruit and other fruit of the Cucurbitaceae family containing mogroside V and other terpene glycosides. The methods employ a cation exchange resin and an anion exchange resin to obtain a sweet juice composition with a clean flavor. The sweet juice composition can be used in a food, beverage, pharmaceutical or dietary supplement product.

The present invention discloses the extractive of Herpetospermum pedunculo-sum (Sex.) Baill as one cucurbitaceous plant and Herpetospermum pedunculo-sum (Sex.) Baill essence, their preparation process, derivative, medicinal composite and medicinal use, especially in preparing medicines for resisting virus, resisting liver damage and resisting tumor. Cell expermiment and animal experiment proves the high activity and less toxicity of Herpetospermum pedunculo-sum (Sex.) Baill essence.

The invention provides methods of inhibiting the growth of a shoot apical meristem of a cucurbit rootstock plant, comprising contacting a shoot apical meristem of the cucurbit rootstock plant with an effective amount of a composition comprising one or more fatty alcohols, thereby inhibiting the growth of the shoot apical meristem of the cucurbit rootstock plant. The present invention further provides methods for preparing and producing cucurbit rootstock plants for grafting and methods for grafting. Additionally provided are cucurbit rootstock plants and plant parts and grafted cucurbit plants produced by the methods of the invention.

The present invention provides a ceramidase activity inhibitor which inhibits ceramidase activity, specifically neutral / alkaline ceramidase activity, characterized in that the inhibitor comprises, as an active ingredient, a processed product derived from at least one plant selected from the group consisting of plants belonging to Ginkgoaceae, plants belonging to Cucurbitaceae, plants belonging to Rutaceae, plants belonging to Laminariaceae, plants belonging to Myrtaceae and plants belonging to Compositae, and a medicament, a quasi-drug, cosmetics and a food, each comprising the inhibitor.

The invention provides a preparation method of a cucurbitacin type compound. The preparation method comprises the steps of extracting pedicellus melo by using an organic solvent, performing solvent distribution on an extract, and performing silica gel column separation and purification by high performance liquid chromatography on a sample after concentration to obtain a cucurbitacin mixture and a cucurbitaceae type compound. Through an anti-aging yeast model-K6001 yeast cell, an active ingredient, namely the cucurbitacin type compound is proved to be capable of significantly prolonging the replicative life span of yeast, so that the cucurbitacin mixture and the cucurbitaceae type compound can be applied in preparation of medicaments for preventing and treating aging and preventing or / and treating age-related diseases. Simultaneously, the cucurbitacin mixture and the cucurbitaceae type compound can also be applied in preparation of nutritional compositions for delaying aging and preventing age-related diseases. The compound can also be used as a lead compound for optimizing the structure and can be applied in preparation of medicaments for delaying aging and preventing or / and treating age-related diseases. The general formula of the structure of the compound is shown in the specification.

The present invention provides a ceramidase activity inhibitor which inhibits ceramidase activity, specifically neutral / alkaline ceramidase activity, characterized in that the inhibitor comprises, as an active ingredient, a processed product derived from at least one plant selected from the group consisting of plants belonging to Ginkgoaceae, plants belonging to Cucurbitaceae, plants belonging to Rutaceae, plants belonging to Laminariaceae, plants belonging to Myrtaceae and plants belonging to Compositae, and a medicament, a quasi-drug, cosmetics and a food, each comprising the inhibitor.

A method of preparing a natural sweetener from a heat-processed powdered fruit extract of the cucurbitaceae family, such as luo han guo is provided. In one embodiment, the method provides a cleaner tasting and concentrated natural sweetener in which objectionable flavors, odors, colors, and insoluble components are removed to form the natural sweetener.

The invention relates to a bitter gourd planting method and belongs to the technical field of agricultural product planting. The bitter gourd planting method mainly comprises the following steps: selecting an environment, selecting varieties, carrying out planting management and carrying out prevention and control on plant diseases and insect pests. A production base needs to be selected from clay loam or sandy loam plots which has or have not been used for planting cucurbitaceae crops for more than three years, and has or have deep soil layers, are loose and fertile and are convenient to irrigate and drain; the varieties are selected from light green or white green varieties which have disease resistance, good quality, abundant yield, storage resistance and good commodity, and are suitable for market requirements; the planting management mainly comprises soil preparation and fertilization application, seed sowing, seedling management, cultivation density, field planting and field management; the prevention and control on the plant diseases and insect pests abide by the policy of main prevention and comprehensive management; a prevention and control method combining agricultural prevention and control, physical prevention and control, biological prevention and control and chemical prevention and control is adopted so that the occurrence of plant diseases and insect pests is controlled; and the method is suitable for planting various bitter gourd varieties, the planting method is simple and easy to operate and has a wide applicable range, and bitter gourds obtained by the method have high yield and few plant diseases and insect pests.

The fast cucurbitaceae RIP gene screening method includes designing degenerate primer pair LY1 / LY2 based on highly conservative amino acid sequence and its codon bias in the upstream and downstream of known cucurbitaceae RIP, Touchdown-PCR amplification with total DNA as template, direct PCR product clonal sequencing of cucurbitaceae plant with un-reported RIP gene to obtain new RIP gene; and fast distinguishing known and unknown RIP genes of cucurbitaceae plant with reported RIP gene according to the length polymorphism of limiting segment in RIP gene LY1 / LY2 segments. Compared with available method of first separating RIP and then cloning gene, the method of the present invention has the features of being fast and accurate, and may be used widely in RIP gene screening.

The invention provides a nutritive staple food suitable for diabetics to eat. The nutritive staple food comprises cereal powder, grain germ powder, food-borne protein powder, nut seed powder and cucurbitaceae vegetable powder. The nutritive staple food is created in the manner of synergistic adding of three nutrients of the grain germ flour, the nut seed powder and the cucurbitaceae vegetable powder, so that the nutritive staple food has good effect on restraining of high blood sugar; and the nut seed powder and the cucurbitaceae vegetable powder are combined for use, so that improvement of human body blood sugar and balance of insulin are further facilitated, and the nutritive staple food has good health-care effects on type II diabetes. Besides, the nutritive staple food is rich in nutrient substances of protein, fat, cellulose, vitamins, mineral trace elements and the like. After being eaten, the nutritive staple food can also supplement comprehensive nutrient substances for organisms, can replace conventional staple foods, and can achieve the best efficacy. The nutritive staple food completely conforms to a principle of Guidelines for Medical Nutrition Therapy for Diabetes in China, and has great significance on extraction of daily nutrients and control of blood sugar for the diabetics.

The invention discloses an establishment technology of a hemsleya amabilis tissue culture system. Hemsleya amabilis is a cucurbitaceous plant and tuberous roots are dried. The hemsleya amabilis is produced in Yunnan, Sichuan, Guizhou, Guangxi and the like. The hemsleya amabilis is dug when leaves become yellow in late autumn; over-ground parts and silt are removed; then the hemsleya amabilis is washed, sliced or not, and dried in the sun. The tuberous roots are of an irregular round shape or oval shape and are of a slight tortoise shape in the vertical direction; the diameter of the large tuberous roots can reach 20cm. Peels are brown yellow or grey brown and are slightly rough and certain peels have concave caudex traces; sections of the peels are light white or grey brown. The hemsleya amabilis is firm and is almost powdery. The hemsleya amabilis has slight odor and a slightly bitter taste. The reproduction speed and the scale are improved by utilizing a plane tissue culture technology, so that industrialized production of hemsleya amabilis germchits is realized. Hemsleya amabilis tissue culture regenerated plants are successfully obtained by taking stems with buds as explants through steps of inducing, reproducing, rooting, carrying out seedling exercising and transplanting and the like, and a systemic hemsleya amabilis tissue culture plant regeneration system is established. The reproduction coefficient is high, the seedling rate is high and the benefits are great.

The present invention is related to an extract of Cucurbitaceae family plant or the purified extract isolated therefrom having anti-adipogenic and anti-obesity activity, and a composition comprising the same. The extract of Cucurbitaceae family plant of the present invention showed potent reducing activity of body weight, decreasing effect on the blood triglyceride and cholesterol level, activating activity of PPAR α and δ, reducing activity of the gene expression of stearoyl-CoA desaturase, and preventing activity from the adipogenesis of precursor fat cells with no toxicity, therefore, those extract can be useful in treating or preventing obesity and adipogenesis-involved diseases as a medicine or health care food.

The invention provides a cola capable of enhancing organism immunity, lowering blood lipid, moisturizing lung and calming nerves, and is characterized by the main components according to the parts by weight: 10-200 of gynostemm, 10-200 of lily, 0.1-0.6 of cola essence, 0.1-2 of caramel pigment, 0.1-2 of phosphoric acid and the balance of flavoring agent, preservative and water. The gynostemm is aleaf of sweetleaf gynostemm of cucurbitaceae, the origin of which is the western area of Hubei province. The gynostemm specially contains trace element selenium. The cola contains trace element selenium, can effectively enhance organism immunity, has significant improving effect to people with dysphoria and fire excess, insomnia and more dreams, and also has significant improving effect on hyperlipidemia symptom.

The invention builds a new VIGS vector capable of being applied to cucurbit plants based on a CGMMV (cucumber green mottle mosaic virus), and provides a CGMMV recombinant vector carrying PDS (phytoenedesaturase) gene segments. The cucurbit plants such as watermelons, sweet melons, cucumbers and bottle gourds are natural hosts of the CGMMV. The recombinant vector can effectively reduce the expression level of a PDS gene in the cucurbit plants, so that the plant generates a PDS gene silenced light bleaching phenotype. The successful building of the recombinant vector can be used for researchingthe gene function; the foundation is laid for digging a cucurbit plant gene with important agronomic trait, and culturing excellent, disease-resistant and specific varieties.

The invention relates to a method for producing seeds by adopting cucumis melo facility in a greenhouse. According to the method, seeds of cucumis melo are produced in the solar greenhouse in early spring, the ratio of male and female parents is 1:5, male parents are 7 days earlier sown than female parents, planting and thorough watering are performed after a seedling has three leaves and one bud, intertillage is performed, a mulching film is covered, single stem training is performed on the female parents, vine-hanging cultivation is performed, pinching is performed when the seedling has 20-22 leaves, 16-19 nodes are used for fruit reserving, 3-4 vines are reserved for the male parents and hung, lateral buds are removed, 3-4 lateral vines are selected for pollination, emasculation is performed on female flowers at four o'clock in the afternoon one day earlier before the female flowers blossom, male flowers are well sleeved with sleeves, the well-sleeved male flowers are picked up at 5-7 o'clock next day, the emasculated female flowers are subjected to cross pollination at 7-10 o'clock and well sleeved with bags after pollination is completed, three hybrid melons are reserved, and two melons are selected when the three hybrid melons grow to the size of an egg. According to the method, the greenhouse is used for seed breeding of the cucumis melo, the flowering periods of wild species of various cucurbitaceae and vegetables are avoided, cross breeding probability is reduced, the seed purity is greatly improved, influence of adverse weather on melon growth is reduced, and the produced seed quality is better guaranteed.

The invention provides an easy, convenient and efficient inoculation method for viruses and virus derivatives of cucurbitaceae crops, and belongs to the technical field of biology. The method is suitable for all viruses, capable of being subjected to mechanical inoculation, of cucurbitaceae crops and virus carriers obtained through infectious cloning and modifying of corresponding viruses, and whole operation is completed only in an aperture disc; the aim that plants are effectively infected by the viruses is achieved through stabbing inoculation. Compared with a traditional mechanical inoculation method, the method has the advantages that operation is easy and convenient, efficiency is high, the application range is wide, the inoculation amount is fixed, and an operating period is short; the method is a rapid, easy, convenient and efficient plant virus inoculation method and can be used for large-scale virus biological work, variety resistance screening and using, disease resisting variety breeding and producing of target bioactive substances with the viruses as carriers.

The invention discloses a dry heat sterilizing method of cucurbitaceae stock seeds. The method comprises the steps of treating the harvested cucurbitaceae stock seeds by a dry heat sterilizer in three stages, wherein the temperature of the first stage is 35 DEG C, the temperature of the second stage is 50 DEG C, and the temperature of the third stage is 72 DEG C. The dry heat sterilizing method of cucurbitaceae stock seeds can effectively kill pathogenic bacteria, especially, high-temperature resistant viruses and the like without measures such as chemicals utilization or seed soaking for changing the seed status, the operation is simple, and therefore, the method is suitable for large-scale seed treatment.

The present invention disclosed a medical use of cucurbitane triterpenoids represented by the following formula and isolated from Momordica charantia L. of Cucurbitaceae family in the preparation of medications for prevention and treatment of diabetes and obesity. The above cucurbitane triterpenoids may be acted as a glucose uptake stimulator, an agonist for the translocation of glucose transporter 4(GLUT4) to the cell membrane, and an activator of adenosine monophosphate-activated protein kinase (AMPK). They may have potential for the prevention and treatment of diabetes and obesity.

The invention provides an oral buccal tablet being capable of enhancing immunity, dredging throat and removing throat abscess and pain, comprising the main components according to the parts by weight:30-300 of gynostemm, 5-50 of American ginseng, 12-120 of sterculia lychnophora, and 0.001-0.02 of menthol. The gynostemm is a leaf of sweetleaf gynostemm of cucurbitaceae, the origin of which is thewestern area of Hubei province. The gynostemm specially contains trace element selenium. The origin of the American ginseng is Wisconsin of America. The oral buccal tablet can effectively enhance organism immunity, rapidly eliminate fatigue, and can solve the problems of throat abscess and pain, hoarseness and the like.

The invention is directed to methods of diagnosing a Pseudoperonospora cubensis infection in a cucurbit plant, comprising detecting at least one nucleotide sequence selected from the group of nucleotide sequences of SEQ ID NOs: 1-52, or any combination thereof, in a sample from a cucurbit plant or in an environmental sample, thereby diagnosing a P. cubensis infection. Also provided is a method of selecting a cucurbit plant comprising at least one resistance gene, the method comprising introducing into the cucurbit plant a nucleotide sequence of any one of SEQ ID NOs: 1-52, wherein the expression of the nucleotide sequence produces a hypersensitive response in a cucurbit plant comprising a resistance gene, thereby identifying the cucurbit plant as comprising a resistance gene; and selecting the plant having the hypersensitive response and identified as comprising said resistance gene.

The invention belongs to the technical field of breeding of cucurbitaceae vegetable. An interspecific hybrid F1 (Yong rootstock No. 6) of cucurbit (a melon rootstock) is obtained by hybridizing a female parent of a stable inbred line J03-2-3-2-1-5-7-4 by multi-generation of directed autocopuation screening of cucurbit materials originated from Japan and a male parent of a stable inbred line YH2-3-3-2-1-4-2-6 by multi-generation of directed autocopuation screening of local Lagenaria species produced in Ningbo. The melon rootstock selectively bred with the method provided in the invention meets objectives of breeding and has moderate growth potential, high resistance to die-back and root rot, well-developed roots, strong stalks, strong ability in absorbing nutrient, low degree of vain growth and robust hypocotyls which are not easy to become hollow, and therefore the melon rootstock is favorable for grafting. The melon rootstock has good grafting affinity and strong symbiosis affinity, and a survival rate over 95% is obtained for the grafting of the rootstock onto the watermelon specie ''Zaojia'' (8424). The engrafted melon rootstock is low temperature-resistant, moisture-resistant and barren-resistant; after field planting, the root and overground part of a grafted seedling grows fast and has sound growth potential, and the period of first harvest of the grafted seedling is 5 days earlier than that of a self-rooted seedling, the fruition rate is high and stable, weight of single fruit and fruit shape can substantially maintain the same, melon skin is not thickened, sugar degree of the grafted seedling is higher than that of the self-rooted seedling, sugar degree gradient is small, quality of the watermelon is not affected, and good adaptability is obtained.

The invention provides a black tea drink capable of enhancing organism immunity, lowering blood lipid, losing weight and improving beautifying function, comprising the main components according to theparts by weight: 3-30 of gynostemm, and 1 of black tea. The gynostemm is a leaf of sweetleaf gynostemm of cucurbitaceae, the origin of which is the western area of Hubei province. The gynostemm specially contains trace element selenium, and the black tea is a tea which is made by the complete fermentation process. Proportioned according to the formula, the product is obtained by aseptic filling after ozone sterilization through even mixing. The black tea drink is used for eliminating the symptoms of being susceptible to fatigue due to low immunity and the like, can effectively remove the freeradicals in human body, resist oxidation, improve the beautifying function and keep youthfulness, delay senility and relieve the discomfort which is caused by electromagnetic wave and the like by long-term drinking, and does not have side effects. The black tea drink has the advantages of simple production process, less equipment investment, low product cost, and can be suitable for large-scale industrialization and specialized production.