12 results about "Cucurbitaceae" patented technology

The invention relates to an improved embryo rescue technology for cucumber haploid breeding, and aims to solve the problem of low embryo rescue operation efficiency in a technology of inducing haploid by radiation pollen. By using a male parent material carrying a green fluorescent protein (GFP) marker, the haploid screening process in the seedling stage is simplified, and efficient and simple large-scale haploid cultivation is realized in combination with an optimized rapid embryo rescue method. The method comprises the specific steps of radiation pollen preparation, pollination and subsequent embryo rescue processes, particularly emphasizes the technical key points of disinfection, seed taking, cleaning and inoculated culture, and ensures that the operation speed and the success rate are improved while the pollution risk is strictly controlled. Compared with a traditional method, the method has the advantages that the obtaining efficiency of haploid cucumber plants is remarkably improved, the method is suitable for large-scale haploid breeding of cucurbitaceae crops, breeding of new cucumber varieties is accelerated, and agricultural yield increase and income increase of farmers are promoted.

The present application relates to the technical field of plant induction regeneration, and particularly relates to a method for improving artificial propagation efficiency of Trichosanthes cucumerina var. villosa, which uses Trichosanthes cucumerina var. villosa stem segments as explants and optimizes the proliferation medium, fixes the excellent traits of the female parent to the greatest extent through direct organogenesis, avoids the variation possibly caused by indirect organogenesis, and has a proliferation cycle of 40 days and a propagation coefficient of more than 14.0; the method meets the needs of large-scale production of high-quality Trichosanthes cucumerina var. villosa seedlings, solves the bud tip necrosis phenomenon commonly existing in in vitro rapid propagation of cucurbitaceae plants, improves the quality of seedlings, and improves the economic benefits; the present application has important significance and value for artificial rapid propagation of Trichosanthes cucumerina var. villosa, and lays a technical foundation for protecting the wild resources and developing artificial planting.

The invention provides a CsBCH1 gene for regulating formation of light yellow petals of cucumber and an InDel marker and application of the CsBCH1 gene, the CsBCH1 gene is a key gene for regulating formation of light yellow petals of cucumber, the function relates to petal carotenoid metabolism, and a new target is provided for research on flower color of cucurbitaceae. The co-dominant InDel marker in the gene can directly and accurately distinguish a homozygous wild type, a homozygous mutant type and a heterozygous type, and the detection method only needs conventional PCR and electrophoresis, and is simple, convenient, rapid and low in cost.

The invention belongs to the technical field of gene engineering, and particularly discloses application of an epidermal hair regulation and control gene in regulation and control of bemisia tabaci resistance. Based on different infection and damage degrees of bemisia tabaci on 384 parts of upland cotton in a cotton field and by utilizing the data to carry out whole genome association analysis, the inventor preliminarily determines that the GhHD1 finally affects the infection degree of the bemisia tabaci on plants by affecting the number of epidermal hairs. Gene knockout and overexpression experiments are further combined to prove that the GhHD1 can obviously increase the number of epidermal hairs of cotton, but obviously enhance the infectivity to bemisia tabaci. Besides, related verification is carried out in representative crops tomato and cucumber of solanaceae and cucurbitaceae, the number of epidermal hairs is further indicated to be positively correlated with the infectivity of bemisia tabaci, and the mechanism in plants is indicated to be conservative. By combining the results, a good gene genetic resource foundation can be laid for cultivating crops of new bemisia tabaci resistance varieties by utilizing the characteristics of plants.

The present application belongs to the technical field of plant molecular biology and plant genetic engineering, and particularly relates to the regulation of McCAB1 gene on the peel color of Momordica charantia. The present application finds that the McCAB1 gene from the green Momordica charantia B07 has a regulation on the fruit color of Momordica charantia. After the McCAB1 gene is heterologously overexpressed, the peel color of Momordica charantia is obviously deepened and green, and the chlorophyll content is also significantly improved. The present application firstly determines that the McCAB1 gene from the green Momordica charantia B07 is a key gene for the regulation of Momordica charantia fruit color, fills the research gap in the field of molecular regulation of Momordica charantia fruit color, and provides a core target for analyzing the formation mechanism of Momordica charantia fruit color. In addition, the McCAB1 gene can be used for the identification of Momordica charantia peel color or directed for the breeding of Momordica charantia fruit appearance quality, helps to cultivate Momordica charantia varieties with excellent color and luster to meet the market demand, and also provides a reference for the improvement of fruit color of Cucurbitaceae crops, and has a wide application prospect.

The invention discloses a method for detecting cucumber green mottle mosaic virus (CGMMV) in soil aiming at the detection technical requirements of important plant pathogens in the soil. The method comprises the following steps: sampling to-be-detected field soil, adding clear water with the same weight, stirring until soil particles are uniform, standing, filtering supernatant liquid, adding polyethylene glycol (the molecular weight is 6000-8000, and the final concentration is 6-10%) and sodium chloride (the final concentration is 1-2%), carrying out ice bath treatment for 2 hours to overnight, centrifuging 12000 g of the solution for 1 hour to settle virus particles, and dissolving and precipitating with distilled water to obtain virus-enriched liquid; the CGMMV can be directly detected by using various conventional detection methods, including an RT-PCR (Reverse Transcription-Polymerase Chain Reaction) method, a fluorescent quantitative PCR method, a colloidal gold immunochromatography method, enzyme-linked immunosorbent assay (ELISA) and the like. According to the method, on the premise of ensuring the reliability and the accuracy, the detection steps are simplified, and the cost and the time are saved. The method is applied to detection of the CGMMV in soil of a cucurbitaceae crop planting field, can effectively guide disease prevention and control in production, and prevents diffusion and harm of the CGMMV.

The invention relates to a Trichoderma longibrachiatum strain TV26, a fungicide containing the Trichoderma longibrachiatum strain TV26 and application of the The trichoderma longibrachiatum strain TV26 has an efficient and lasting antagonistic effect on cucurbitaceae, and can play a key role in biological prevention and control of cucumber spot disease. Furthermore, by researching endogenous metabolites of the strain, it is found that the volatile metabolite pinobiose of the strain serves as a synergist, the colonization ability of the strain on cucumber leaves is remarkably improved, and therefore the strain can be used for developing a more efficient compound microbial agent. The bacterial strain and the compound bacterial agent have the characteristics of high efficiency, durability, safety and environmental protection when being used for antagonizing cucurbitaceae and preventing and treating cucumber spot disease caused by cucurbitaceae, so that the bacterial strain and the compound bacterial agent have relatively high industrial value and application prospect.

The application relates to a Burkholderia gladioli NDG19 and an application of the Burkholderia gladioli NDG19 in disease resistance, and belongs to the technical field of microorganisms. The Burkholderia gladioli NDG19 is preserved in the Guangdong Provincial Microbial Culture Collection Center on July 31, 2023, the strain preservation number is 63701, and the preservation address is No. 59 Building, 5th Floor, Guangzhou, Guangzhou, China. The Burkholderia gladioli NDG19 provided in the application can effectively inhibit the growth and reproduction of Fusarium oxysporum and Alternaria tenuis, and can effectively prevent and treat plant fusarium wilt such as sharp banana fusarium wilt and melon root rot of cucurbitaceae crops, solves the problems of environmental pollution and food safety caused by chemical fungicides, the strain has the functions of iron carrier production, phosphorus dissolution and potassium dissolution, can effectively improve the utilization rate of phosphorus and potassium in soil as a biological bacterial fertilizer, promotes the growth of plants, is safe and environment-friendly, and has a wide application prospect.

The present invention relates to a modified ABCB9 gene encoding a protein conferring resistance to CMV in a plant of the Cucurbitaceae, such as a cucumber plant, in which the protein is expressed, where the gene has a) a nucleotide sequence which encodes a protein with SEQ ID NO: 4; b) a nucleotide sequence with SEQ ID NO: 3; c) a nucleotide sequence encoding a protein derived by substitution, deletion and / or addition of one or more amino acids of the protein with SEQ ID NO: 4; d) a nucleotide sequence that encodes a protein with an amino acid sequence, which is at least 85% identical to SEQ ID NO: 4; e) a nucleotide sequence which is at least 85% identical to SEQ ID NO: 3; or f) a nucleotide sequence according to c) or d) wherein the protein has a methionine (M) on position 428 of SEQ ID NO: 4, or on a position corresponding thereto.

This utility model discloses an automatic crop harvesting device, belonging to the field of automated crop harvesting technology. The utility model includes a carrier and a support plate mounted on the carrier's bearing surface. The carrier is a motorized vehicle. A support and a drive assembly are mounted on the support plate to move the support along the length of the support plate. A picking component and a collecting component are connected to the mounting surface of the support. The picking component cuts the crop's rootstock; the collecting component below the picking component collects the cut fruit. In practical use, the support plate provides an installation platform for the support and drive assembly. The drive assembly enables the support to move along the length of the support plate. The picking component is responsible for cutting the rootstock of the cucurbitaceous crop, separating the fruit from the plant. The collecting component collects the fruit promptly after cutting. With the cooperation of the carrier, picking and collecting are integrated into one unit. This solves the technical problem of low picking and collecting efficiency of cucurbitaceous crops in the prior art.

The application discloses a melon auxin receptor gene CmTIR1, a coded protein thereof, a recombinant expression vector, a genetic transformant, a method for regulating plant height and application in molecular breeding, and belongs to the technical field of plant genetic engineering. The melon auxin receptor gene CmTIR1 is MELO3C015898, the nucleotide sequence of which is shown as SEQ ID No. 1, and the amino acid sequence of the coded protein is shown as SEQ ID No. 2. After the CmTIR1 gene is knocked out by using the CRISPR / Cas9 technology, the melon plant height is significantly reduced, and the number of internodes is reduced; and overexpression of the gene is characterized in that the plant height is significantly increased. The application first discloses the core role of the CmTIR1 gene in the regulation of melon plant height, provides a new gene resource and a theoretical basis for the plant type improvement and molecular breeding of melon and other cucurbitaceae crops, and has practical application value and wide application prospect.

The application discloses a kind of cucurbitaceae sphingomonas SPZ1 and its complex microbial inoculant in application of promoting peanut plant photosynthetic physiology and nitrogen metabolism, belong to the field of microbial technology.The active bacteria of the present application is first composed of plant Arthrobacter ARZ1 (Arthrobacter oryzae) and cucurbitaceae sphingomonas SPZ1 (Sphingomonas melonis) complex microbial inoculant is applied to peanut planting field, the two bacteria in the complex microbial inoculant have obvious improvement on the maximum photochemical efficiency of peanut plant and leaf chlorophyll SPAD value through synergistic effect, especially can improve the activity of whole peanut leaf nitrogen metabolism enzyme (nitrate reductase and glutamine synthetase), so as to be applied to promote peanut plant photosynthetic physiology and nitrogen metabolism.