53 results about "Alternaria solani" patented technology

The invention belongs to the technical field of gene engineering, and relates to application of a potato late blight bacterium response gene StRTP5 in regulation and control of potato disease resistance. The invention provides application of a potato late blight response gene StRTP5 in regulation and control of potato disease resistance, the potato late blight response gene StRTP5 comprises a gene StRTP5a and a gene StRTP5b, the gene StRTP5a and the gene StRTP5b negatively regulate and control resistance of potatoes to phytophthora infestans and alternaria solani, and the gene StRTP5a and the gene StRTP5b have potential to be used for creating potato broad-spectrum disease-resistant materials.

The invention belongs to the field of microorganisms, and particularly relates to bacillus velezensis with high stress resistance, bacterial powder, a biocontrol microbial inoculum and preparation and application thereof. The bacillus velezensis is bacillus velezensis Shannan.BV80-12 and is preserved in the China Center for Type Culture Collection, and the preservation number of the bacillus velezensis is CCTCC (China Center for Type Culture Collection) NO: M 2024386. The bacillus velezensis Shannan.BV80-12 provided by the invention has relatively strong tolerance to drought, and has the capability of producing indoleacetic acid (IAA). Meanwhile, the bacterial strain also has a broad-spectrum antibacterial ability, and has a relatively strong inhibition effect on fusarium graminearum, alternaria alternata, magnaporthe oryzae, bipolaris maydis, exserohilum turcicum, sclerotinia sclerotiorum, physalospora piricola, alternaria solani and citrus brown rot pathogen.

The present invention relates to methods for controlling or preventing infestation of vegetable, tomato and potato plants by phytopathogenic microorganisms selected from Sphaerotheca fuliginea, Leveillula taurica, Sclerotinia sclerotiorum, Cercospora, Fusarium oxysporum, Fusarium solani, Helminthosporium solani, Phoma tuberosa, Rhizoctonia solani, Phytophthora infestans, Verticillium dahlia, Didymella bryoniae, Botrytis cinerea and Alternaria solani, comprising applying to a crop of plants, the locus thereof, or propagation material thereof, a compound according to formula (I) wherein R1, R2, R3, R4, R5, Y, A, B are as defined herein.

The invention belongs to the technical field of microorganisms, and discloses bacillus velezensis and application thereof. The strain is separated from saline-alkali soil in the Uygur autonomous region in Xinjiang and is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No.65485. By utilizing a response surface optimization method, the cellulase activity reaches 92.376 U / mL and is improved by 95.6% compared with that of a non-optimized cellulase activity; the growth inhibition rate on alternaria solani, alternaria alternate, fusarium oxysporum and botrytis cinerea hyphae is 88.47-91.08%, and the metabolite (especially an ethyl acetate extract) can significantly inhibit Korla pear diseases caused by alternaria alternate. The strain is resistant to high and low temperature, acid and alkali and salt stress, can degrade straw (the degradation rate is 21.9%), and provides a high-quality strain resource for resource utilization of agricultural wastes and biological control of plant fungal diseases.

The invention provides a vanillin compound spliced by polyheterocycles. The polyheterocycles are one of a benzimidazole ring, a thiadiazole ring, an oxadiazole ring and a thiazole ring; the vanillin compound spliced by polyheterocycles is one of vanillin derivatives containing a benzimidazole ring, a thiadiazole ring, an oxadiazole ring and a thiazole ring, and the chemical general formula is shown in the specification. The polyheterocycle spliced vanillin compound is prepared through a condensation reaction and a condensation-cyclization reaction, and the compound has good antibacterial activity on valsa mali, red kidney bean root rot, fusarium graminearum, millet pyricularia grisea, botrytis cinerea, alternaria solani and watermelon fusarium oxysporum. The compound can be used for preparing a novel efficient antibacterial agent and is applied to prevention and treatment of the pathogenic bacteria.

A microbial composite strain H2, antagonistic to multiple potato pathogens, and its application and preparation method, relates to the field of biological control. The microbial composite strain H2 is a mixture of Bacillus subtilis, Bacillus atrophaeus, and Bacillus Widmannii. It exhibits strong inhibitory effects against potato early blight pathogens Alternaria solani and Alternaria alternata; wilt / dry rot pathogens Fusarium solani, Fusarium oxysporum, and Fusarium equiseti; and leaf / stem spot pathogen Vitis vinifera, with inhibition rates exceeding 46%. The highest inhibition rate against Alternaria alternata reached 75.97%. H2 also exhibits significant growth-promoting and yield-increasing effects, providing a high-quality strain for the comprehensive control of soil-borne potato diseases and promising prospects for field application.

The invention discloses a hydrazide compound taking syringic acid as a lead as well as a preparation method and application of the hydrazide compound, and a biological activity test result shows that all the compounds have excellent and broad-spectrum antibacterial activity on tested plant pathogenic fungi. Wherein the inhibition effect of most target compounds on plant pathogenic fungi is superior to that of positive control boscalid, the compound 11 has the best antibacterial effect on rhizoctonia solani, fusarium graminearum, alternaria solani, colletotrichum frutescens and colletotrichum sorghum, and EC50 values of the compound 11 are respectively 0.258 [mu] g / mL, 0.679 [mu] g / mL, 0.424 [mu] g / mL, 0.384 [mu] g / mL and 0.975 [mu] g / mL; for colletotrichum gloeosporioides, the compound 12 has the best antibacterial effect, and the EC50 value of the compound 12 is 0.989 mu g / mL; and secondly, a compound 11 with an EC50 value of 1.248 [mu] g / mL. The hydrazide compound prepared by taking syringic acid as a lead has excellent, broad-spectrum and efficient plant pathogenic microorganism resisting activity, and can be used for preventing and treating plant diseases caused by plant pathogenic microorganisms.

The invention relates to the technical field of natural medicinal chemistry, and discloses a compound M-1 with phytopathogen resisting activity and application thereof. The invention provides a novel compound and a fermentation liquor separated from a Ralstonia MWSTR-5 strain, and by measuring the inhibitory activity of the compound M-1 on fusarium oxysporum and alternaria solani, the compound M-1 is found to have relatively strong phytopathogen resistance activity and is a good natural bactericide.

The invention discloses a dehydroabietyl quaternary ammonium salt of which the chemical structural formula is shown in the following formula. The dehydroabietyl quaternary ammonium salt can be used for effectively inhibiting the growth of valsa mali, Phytophthora capsici, Fusarium graminearum, Fusarium oxysporum f.sp.niveum and Alternaria solani, and the dehydroabietyl quaternary ammonium salt can be used for effectively inhibiting the growth of valsa mali, Phytophthora capsici, Fusarium graminearum, Fusarium oxysporum f.sp.niveum and Alternaria solani. The method has potential value in preparation of green pesticide bactericides, and can reduce adverse effects of the bactericides on the environment and human beings; # imgabs0 #

This invention belongs to the field of biodetection technology, specifically relating to a primer set, kit, and application for detecting *Alternaria solani* brown spot disease. The base sequences of the primer set are shown in SEQ ID NO.1~SEQ ID NO.5. Based on the primer set, this invention utilizes loop-mediated isothermal amplification technology for the rapid detection of *Alternaria solani*, accurately detecting the fungus from the complex pathogenic environment in diseased plant tissues. The rapid detection system described in this invention exhibits high specificity and sensitivity, with a detection limit of 0.246 fg / μL for *Alternaria solani* DNA. Using the rapid detection system provided by this invention, *Alternaria solani* can be detected rapidly, conveniently, accurately, and sensitively from field samples within 45 minutes, which is simpler and more efficient than traditional symptom identification methods and ordinary molecular detection methods.

The invention discloses a novel methoxyimino acetate derivative containing a chloroacetamide structure as well as a preparation method and application of the novel methoxyimino acetate derivative. According to the invention, a methoxyimino acetate skeleton is used as a leading structure, and different pharmacophores are introduced on the basis of the leading structure, so that a series of novel methoxyimino acetate skeleton derivatives containing a chloroacetamide structure are synthesized. In-vitro tests of the compounds show that the compounds have excellent inhibition effects on plant pathogenic fungi such as fusarium graminearum, eggplant verticillium wilt, mango anthracnose pathogen, alternaria solani, sclerotinia sclerotiorum, muskmelon fusarium wilt pathogen and the like; the methoxyl acrylate derivative pesticide with high activity and novel action mechanism is expected to be developed.

The invention discloses a microbial compound bacterium H2 for antagonizing multiple pathogenic fungi of potatoes and application and a preparation method of the microbial compound bacterium H2, and relates to the field of biological control, and the microbial compound bacterium H2 is formed by mixing bacillus subtillis, bacillus atrophaeus and bacillus vedmannii. The compound has a relatively strong inhibition effect on potato early blight pathogenic fungi alternaria solani and alternaria alternata, fusarium solani, fusarium oxysporum and fusarium equisetum pathogenic fungi of fusarium wilt / dry rot, and leaf / stem leaf spot pathogenic fungi grape stem blight, and the inhibition rates are all higher than 46%. Wherein the inhibition rate on alternaria alternata is the highest and reaches 75.97%. Meanwhile, H2 has good growth-promoting and yield-increasing effects, a high-quality strain is provided for comprehensively preventing and treating potato soil-borne diseases, and the field application prospect is good.

The invention belongs to the technical field of molecular traceability of plant pathogenic bacteria, and discloses a molecular traceability method of potato stripe bacteria, which comprises eight specific SNP (Single Nucleotide Polymorphism) marker combinations located in the 80226-81462 interval of a CaLso genome, a nested PCR (Polymerase Chain Reaction) primer group for detecting the marker combinations, and a molecular traceability kit containing the primer group. The invention relates to a potato stripe pathogen molecule traceability method. The method comprises the following steps: sample DNA extraction, qPCR positive screening, nested PCR amplification, SNP site sequencing comparison and genotype analysis, so as to realize accurate determination of CaLso geographical source and haplotype. The method can be applied to CaLso quarantine and intrusion risk prevention and control of commercial crop seeds of Solanaceae or Umbelliferae imported from ports, provides technical support for monitoring pathogen propagation paths and formulating targeted prevention and control strategies, and is high in practicability.

The invention belongs to the technical field of biological detection, and particularly relates to a primer group and a kit for detecting new plum brown spot and application of the primer group and the kit, and base sequences of the primer group are shown as SEQ ID NO.1-SEQ ID NO.5. Based on the primer group, the loop-mediated isothermal amplification technology is used for rapid detection of the alternaria nepalensis, and the alternaria nepalensis can be accurately detected from a complex pathogenic bacterium environment in diseased plant tissues. The rapid detection system disclosed by the invention is good in specificity and high in sensitivity, and the detection limit of the DNA of the alternaria nepalensis is 0.246 fg / mu L. By utilizing the rapid detection system provided by the invention, the alternaria nepalensis can be rapidly, simply, accurately and sensitively detected from a field sample within 45 minutes, and compared with a traditional symptom recognition method and a common molecular detection method, the rapid detection system is simpler and more efficient.

The application discloses a kind of hydrazine compounds with eugenol as lead and its preparation method and application, and the biological activity test result shows that all compounds are excellent, broad-spectrum antibacterial activity to the plant pathogenic fungi tested.The inhibition effect of most target compounds on plant pathogenic fungi is better than that of positive control rynaxapyr, and the antibacterial effect of compound 11 on rhizoctonia solani, fusarium graminearum, alternaria solani, phoma exigua var. horii and phoma sorghicola is the best, and the EC 50 Values are 0.258 μg / mL, 0.679 μg / mL, 0.424 μg / mL, 0.384 μg / mL and 0.975 μg / mL respectively; for the gossypium gossypium, the antibacterial effect of compound 12 is the best, and the EC 50 Value is 0.989 μg / mL; followed by compound 11, and the EC 50 Value is 1.248 μg / mL.The hydrazine compounds prepared with eugenol as lead have excellent, broad-spectrum and high-efficiency anti-plant pathogenic microorganism activity, and can be used for preventing and treating plant diseases caused by plant pathogenic microorganisms.

The application discloses a coumarin derivative, a preparation method and application thereof, and has a structure as shown in formula (I) and / or (II). The method for preparing the coumarin derivative is simple and efficient, and the application is that the prepared coumarin derivative has good in-vitro inhibitory activity on four common plant pathogenic fungi of Botrytis cinerea, Fusarium oxysporum, Alternaria alternata and Alternaria solani. The compound has good application in a plant-derived fungus inhibitor, is more environmentally friendly, and meets the green chemistry concept.

The invention discloses a phenylaminothiazole derivative as shown in a formula I and application of the phenylaminothiazole derivative to prevention and treatment of plant diseases. The phenylaminothiazole derivative has the advantages of simple and easily available raw materials, simple preparation method, convenient post-treatment and high product yield, and the compound as shown in the formula I has broad-spectrum and efficient anti-plant fungus activity. The bactericidal composition especially has a good bactericidal effect on rhizoctonia solani, sclerotinia sclerotiorum, alternaria solani, botryosphaeria dothidea, apple black beancurd skin fungi, rhizoctonia cerealis, fusarium graminearum and colletotrichum gloeosporioides. # imgabs0 #

The invention belongs to the technical field of biological agents, and particularly relates to trichoderma harzianum, a biocontrol agent, a bio-organic fertilizer, a method for preventing and treating plant diseases and application. The invention provides a trichoderma harzianum P11 with the preservation number of CCTCC NO: M 2024648. The trichoderma harzianum P11 can improve the relative expression quantity of disease-resistant positive correlation genes PnCAD, PnSN1 and PnDEFL1 in plants and improve the activity of disease-resistant related enzyme activities SOD, POD and CAT, and the trichoderma harzianum P11 can damage cell membranes of pathogenic bacteria and inhibit the growth of the disease pathogenic bacteria. The trichoderma harzianum P11 has good rhizosphere colonization capability. The result of the embodiment shows that the trichoderma harzianum P11 can reduce the morbidity of panax notoginseng field root rot, and can also antagonize ginseng alternaria alternata, magnaporthe oryzae, polygonatum kingianum colletotrichum, geotrichum candidum, alternaria solani, cylindrosporium destroyed, phytophthora capsici and conidiospora spiraea.

The application discloses a bacillus velezensis BZWQ55, a microbial agent and application thereof, and belongs to the field of microorganisms. The bacillus velezensis BZWQ55 is screened from rhizosphere soil of potato scab and has a preservation number of CGMCC No. 36805. Experimental results show that the bacillus velezensis BZWQ55 has the abilities of producing cellulase, protease, amylase and iron carrier, and has the abilities of phosphorus and potassium solubilization and nitrogen fixation, and can effectively promote plant growth; the bacillus velezensis BZWQ55 can also stably inhibit the growth of Fusarium solani, Fusarium oxysporum, Alternaria solani, Streptomyces scabrisporus, Rhizoctonia solani and Neocosmospora vasinfecta, and has a significant prevention effect on multiple soil-borne diseases such as potato scab, sweet potato root rot, potato scab and peanut fruit rot. The application provides a new strain resource for biological control of plant soil-borne diseases and promotion of plant growth.

The invention discloses application of imidazo [1, 2-a] pyridine derivatives in prevention and treatment of plant diseases, and a series of imidazo [1, 2-a] pyridine skeleton-containing derivatives are synthesized. The compound has excellent inhibitory activity on botryosphaeria dothidea, colletotrichum gloeosporioides, colletotrichum kaoliforme, colletotrichum frutescens, alternaria solani, fusarium oxysporum, pepper fusarium wilt and rhizoctonia solani. Wherein the EC50 value of a part of the compounds for resisting colletotrichum frutescens, colletotrichum gloeosporioides and capsicum oxysporum is close to that of the positive control drug, namely, partheneine, the EC50 value of the part of the compounds for resisting fusarium oxysporum is superior to that of the positive control drug, namely, partheneine, and the compound also shows good inhibitory activity on plant pathogenic bacteria. In conclusion, the compound disclosed by the invention can be used for preparing a plant fungicide, and has potential application value for plant fungal or bacterial diseases.

This invention discloses a transcription factor gene of Minjiang lily. LrbHLH1 Its nucleotide sequence is as described in SEQ ID NO: 1, encoding a protein with the amino acid sequence shown in SEQ ID NO: 2. This invention has confirmed this through reverse genetics technology. LrbHLH1 The gene has the function of enhancing plant resistance to fungi, and the present invention will... LrbHLH1 Genes were constructed into plant expression vectors and overexpressed in tobacco. The transgenic tobacco plants exhibited strong resistance to fungal infection, demonstrating the effectiveness of overexpression. LrbHLH1 The genetically modified tobacco plants exhibit high levels of resistance to infection by *Alternaria alternata* and *Alternaria solani*.

A bacterial strain antagonistic to multiple pathogenic fungi of potato and its application, which relate to the field of biological control, the strain is Bacillus atrophaeus Ba45, which is deposited in the General Microbiology Center of China Culture Collection Administration, with the deposit address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, the deposit date: November 11, 2024, and the deposit number is CGMCC No. 32577. Bacillus atrophaeus Ba45 is used to prepare a bacterial agent antagonistic to potato pathogenic fungi. The Ba45 of the present invention greatly improves the comprehensive inhibition rate of Alternaria solani, Alternaria alternata, Fusarium solani, Fusarium oxysporum, Fusarium equisetum and grape stem blight. Therefore, Ba45 provides a more resistant high-quality strain for the comprehensive prevention and control of potato soil-borne diseases, and has good application prospects.

The present invention provides a Brevibacillus formosus strain SXM-17, its derivative products and applications in preventing and controlling crop diseases, belonging to the technical field of functional microorganisms. In the present invention, a strain SXM-17 is isolated and purified from wheat plants, and the preservation number is CGMCC No. 30198, which has good inhibitory effects on Gibberella zeae, Physalospora piricola, Botrytis cinerea, Alternaria solani, Rhizoctonia solani and Gaeumannomyces graminis var. tritici, etc. Experiments show that the culture solution, bacterial suspension and supernatant of the strain SXM-17 all have good control effects. In addition, the strain SXM-17 also has a growth-promoting effect on crops planted in natural planting environments and salinized environments. The strain SXM-17 provided by the present invention enriches the resource library of biocontrol strains against Gibberella zeae and the resources of green and efficient biological control microorganisms.

The invention discloses an application of Cu-NPs as a bacteriostatic agent of alternaria solani. The concentration of the Cu-NPs is 100 g * mL <-1 >. The Cu-NPs is low in production cost, safe and simple in application and operation method, experiments prove that the Cu-NPs have broad-spectrum inhibitory activity on plant pathogenic fungi, the antibacterial effect of the Cu-NPs at the concentration of 100 g.mL <-1 > is optimal, and the antibacterial effect of the Cu-NPs is superior to that of broad-spectrum bactericide thiophanate methyl. The antibacterial rate of the Cu-NPs solution with the concentration of 100 g.mL <-1 > on tomato early blight pathogenic bacteria alternaria solani reaches 85%.

The invention discloses bacillus velezensis SGTSH 1011, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No.34265. The invention further discloses a microbial pesticide preparation which comprises the bacillus velezensis SGTSH 1011 parent drug, a dispersing agent, an emulsifying agent, a stabilizing agent, a thickening agent and a dispersing medium. The invention also discloses application of the microbial pesticide preparation in prevention and treatment of sunflower sclerotiniose. The strain has remarkable inhibition activity on sunflower sclerotinia sclerotiorum pathogenic bacteria sclerotinia sclerotiorum, the inhibition rate reaches up to 85.68%, meanwhile, the strain shows a broad-spectrum antibacterial effect on various plant pathogenic fungi such as sorghum colletotrichum, fusarium oxysporum, fusarium graminearum and alternaria solani, and good biocontrol application potential is achieved. By optimizing a preparation formula, the technical problem that a living microbial preparation is easy to inactivate in the storage and transportation processes is solved.

The invention provides bacillus vallismortis TCS004 with a preservation number of CGMCC (China General Microbiological Culture Collection Center) NO.25850. The invention discloses an application of the bacillus vallismortis TCS004 in preventing and treating plant pathogenic fungi or plant pathogenic bacteria, or an application of the bacillus vallismortis TCS004 in preparing a microbial preparation for preventing and treating the plant pathogenic fungi or the plant pathogenic bacteria. The bacterial strain has a very high inhibition effect on plant pathogenic fungi such as lasiodiplodia mali, macrostem botryosphaeria verticillata, citrus interspersonia, streptosclerotinia sclerotiorum, alternaria alternata, colletotrichum frutescens, muscardine fungus, alternaria solani, fusarium solani, botrytis cinerea, fusarium equisetum, curvularia, alternaria alternata and the like; and the compound also has relatively good bacteriostatic activity on plant pathogenic bacteria xanthomonas oryzae and the like. The invention further provides a formula of a fermentation culture medium of the bacillus vallismortis TCS004 and fermentation process conditions of the bacillus vallismortis TCS004.

The invention discloses a coumarin derivative containing a Schiff base structure as well as a preparation method and application of the coumarin derivative in resisting plant pathogenic fungi, and belongs to the field of medicinal chemistry. The coumarin derivative containing the Schiff base structure is generated by taking coumarin as a parent and reacting with the Schiff base containing an azomethyl characteristic functional group, and is used for preventing plant fungal infectious diseases. The coumarin derivative containing the Schiff base structure, provided by the invention, has a good inhibition effect on botrytis cinerea, alternaria solani, fusarium oxysporum and alternaria alternata, and can be used as a lead compound for creating a novel agricultural bactericide.

The application discloses a benzopentaheterocyclic compound containing a hydrazine structure and an application thereof. The compound, stereoisomers of the compound, or a salt of the compound, or a solvate of the compound has good inhibiting effects on plant pathogenic bacteria and fungi. The compound has good inhibiting effects on fungi such as Colletotrichum gloeosporioides, Colletotrichum theae-sinensis, Mycosphaerella pachyrrhizi, Alternaria solani, Phytophthora capsici and the like, and pathogenic bacteria such as Xanthomonas oryzae, Xanthomonas citri and the like. Some of the compounds show excellent inhibiting activities on Colletotrichum theae-sinensis, with an EC 50 of 11.85 μg / mL. Some of the compounds show excellent inhibiting activities on Mycosphaerella pachyrrhizi, with an EC 50 of 2.88-16.41 μg / mL. Some of the compounds show excellent inhibiting activities on Xanthomonas citri, with a primary screening activity of 13.69-27.03 μg / mL, which is superior to a commercial drug, copper oxychloride. Some of the compounds show excellent inhibiting activities on Xanthomonas campestris, with a primary screening activity of 25.07-43.78 μg / mL, which is superior to the commercial drug, copper oxychloride. The application provides an important scientific basis for the research and development of new pesticides.

The invention relates to a preparation method of a supernatant for biological control, which comprises the following steps: fermenting and centrifuging streptomyces fusiformis, and taking the supernatant; the strain name of the streptomyces fusiformis is streptomyces fusiformis JN4-2, and the preservation number of the streptomyces fusiformis JN4-2 is CGMCC (China General Microbiological Culture Collection Center) No. 28157. The supernate can be used for treating Korean pine rhizoctonia solani, red adzuki bean colletotrichum gloeosporioides, symbiotic microporus, apple shell monochromatic alternaria solani, exserohilum turcicum, tobacco target leaf spot, fusarium moniliforme, muskmelon vine cutting pathogen, pepper colletotrichum gloeosporioides, dahurian larch shoot pathogen, blueberry canker pathogen, poplar skin rot pathogen and valsa mali. The biological control agent can be used for biologically controlling pathogenic bacteria such as poplar canker pathogen, eggplant brown rot pathogen, pythium aphanidermatum, alternaria alternata, blueberry branch blight pathogen, apple fruit rot pathogen, cucumber sclerotinia sclerotiorum and the like, and has the advantages of good biocontrol effect, wide antibacterial spectrum, wide application range and the like.

The invention discloses an imidazo pyrimidone-containing derivative and a preparation method and application thereof, and a series of imidazo pyrimidone-containing skeleton derivatives are synthesized by taking an imidazo pyrimidone-containing skeleton as a pilot structure and grafting different pharmacophores on the basis of the pilot structure. In-vitro tests of the compounds show that the compounds have effects on plant pathogenic bacteria such as Xanthomonas oryzae pv. Oryzae, Xanthomonas citri, Xanthomonas oryzae pv. Oryzae and Xanthomonas syringae pv. Actinidia; the compound has an excellent inhibition effect on plant pathogenic fungi such as rhizoctonia solani, colletotrichum chinense, alternaria solani and the like. Especially, the EC50 of most of the halogen-containing compounds to Xanthomonas oryzae pv. Oryzae and Xanthomonas citri pv. Citri is within 10 [mu] g / mL, especially the EC50 of the compound 14 to Xanthomonas oryzae pv. Oryzae is 3.86 [mu] g / mL, the EC50 of the compound 14 to Xanthomonas citri pv. Citri is 4.04 [mu] g / mL, and the halogen-containing compounds have good antibacterial activity.