36 results about "Alternaria solani" patented technology

The invention belongs to the technical field of gene engineering, and relates to application of a potato late blight bacterium response gene StRTP5 in regulation and control of potato disease resistance. The invention provides application of a potato late blight response gene StRTP5 in regulation and control of potato disease resistance, the potato late blight response gene StRTP5 comprises a gene StRTP5a and a gene StRTP5b, the gene StRTP5a and the gene StRTP5b negatively regulate and control resistance of potatoes to phytophthora infestans and alternaria solani, and the gene StRTP5a and the gene StRTP5b have potential to be used for creating potato broad-spectrum disease-resistant materials.

The invention belongs to the technical field of microorganisms, and discloses bacillus velezensis and application thereof. The strain is separated from saline-alkali soil in the Uygur autonomous region in Xinjiang and is preserved in Guangdong Microbial Culture Collection Center, and the preservation number is GDMCC No.65485. By utilizing a response surface optimization method, the cellulase activity reaches 92.376 U / mL and is improved by 95.6% compared with that of a non-optimized cellulase activity; the growth inhibition rate on alternaria solani, alternaria alternate, fusarium oxysporum and botrytis cinerea hyphae is 88.47-91.08%, and the metabolite (especially an ethyl acetate extract) can significantly inhibit Korla pear diseases caused by alternaria alternate. The strain is resistant to high and low temperature, acid and alkali and salt stress, can degrade straw (the degradation rate is 21.9%), and provides a high-quality strain resource for resource utilization of agricultural wastes and biological control of plant fungal diseases.

The invention provides a vanillin compound spliced by polyheterocycles. The polyheterocycles are one of a benzimidazole ring, a thiadiazole ring, an oxadiazole ring and a thiazole ring; the vanillin compound spliced by polyheterocycles is one of vanillin derivatives containing a benzimidazole ring, a thiadiazole ring, an oxadiazole ring and a thiazole ring, and the chemical general formula is shown in the specification. The polyheterocycle spliced vanillin compound is prepared through a condensation reaction and a condensation-cyclization reaction, and the compound has good antibacterial activity on valsa mali, red kidney bean root rot, fusarium graminearum, millet pyricularia grisea, botrytis cinerea, alternaria solani and watermelon fusarium oxysporum. The compound can be used for preparing a novel efficient antibacterial agent and is applied to prevention and treatment of the pathogenic bacteria.

A microbial composite strain H2, antagonistic to multiple potato pathogens, and its application and preparation method, relates to the field of biological control. The microbial composite strain H2 is a mixture of Bacillus subtilis, Bacillus atrophaeus, and Bacillus Widmannii. It exhibits strong inhibitory effects against potato early blight pathogens Alternaria solani and Alternaria alternata; wilt / dry rot pathogens Fusarium solani, Fusarium oxysporum, and Fusarium equiseti; and leaf / stem spot pathogen Vitis vinifera, with inhibition rates exceeding 46%. The highest inhibition rate against Alternaria alternata reached 75.97%. H2 also exhibits significant growth-promoting and yield-increasing effects, providing a high-quality strain for the comprehensive control of soil-borne potato diseases and promising prospects for field application.

The invention relates to the technical field of natural medicinal chemistry, and discloses a compound M-1 with phytopathogen resisting activity and application thereof. The invention provides a novel compound and a fermentation liquor separated from a Ralstonia MWSTR-5 strain, and by measuring the inhibitory activity of the compound M-1 on fusarium oxysporum and alternaria solani, the compound M-1 is found to have relatively strong phytopathogen resistance activity and is a good natural bactericide.

This invention belongs to the field of biodetection technology, specifically relating to a primer set, kit, and application for detecting *Alternaria solani* brown spot disease. The base sequences of the primer set are shown in SEQ ID NO.1~SEQ ID NO.5. Based on the primer set, this invention utilizes loop-mediated isothermal amplification technology for the rapid detection of *Alternaria solani*, accurately detecting the fungus from the complex pathogenic environment in diseased plant tissues. The rapid detection system described in this invention exhibits high specificity and sensitivity, with a detection limit of 0.246 fg / μL for *Alternaria solani* DNA. Using the rapid detection system provided by this invention, *Alternaria solani* can be detected rapidly, conveniently, accurately, and sensitively from field samples within 45 minutes, which is simpler and more efficient than traditional symptom identification methods and ordinary molecular detection methods.

The invention belongs to the technical field of molecular traceability of plant pathogenic bacteria, and discloses a molecular traceability method of potato stripe bacteria, which comprises eight specific SNP (Single Nucleotide Polymorphism) marker combinations located in the 80226-81462 interval of a CaLso genome, a nested PCR (Polymerase Chain Reaction) primer group for detecting the marker combinations, and a molecular traceability kit containing the primer group. The invention relates to a potato stripe pathogen molecule traceability method. The method comprises the following steps: sample DNA extraction, qPCR positive screening, nested PCR amplification, SNP site sequencing comparison and genotype analysis, so as to realize accurate determination of CaLso geographical source and haplotype. The method can be applied to CaLso quarantine and intrusion risk prevention and control of commercial crop seeds of Solanaceae or Umbelliferae imported from ports, provides technical support for monitoring pathogen propagation paths and formulating targeted prevention and control strategies, and is high in practicability.

The invention belongs to the technical field of biological detection, and particularly relates to a primer group and a kit for detecting new plum brown spot and application of the primer group and the kit, and base sequences of the primer group are shown as SEQ ID NO.1-SEQ ID NO.5. Based on the primer group, the loop-mediated isothermal amplification technology is used for rapid detection of the alternaria nepalensis, and the alternaria nepalensis can be accurately detected from a complex pathogenic bacterium environment in diseased plant tissues. The rapid detection system disclosed by the invention is good in specificity and high in sensitivity, and the detection limit of the DNA of the alternaria nepalensis is 0.246 fg / mu L. By utilizing the rapid detection system provided by the invention, the alternaria nepalensis can be rapidly, simply, accurately and sensitively detected from a field sample within 45 minutes, and compared with a traditional symptom recognition method and a common molecular detection method, the rapid detection system is simpler and more efficient.

The application discloses a kind of hydrazine compounds with eugenol as lead and its preparation method and application, and the biological activity test result shows that all compounds are excellent, broad-spectrum antibacterial activity to the plant pathogenic fungi tested.The inhibition effect of most target compounds on plant pathogenic fungi is better than that of positive control rynaxapyr, and the antibacterial effect of compound 11 on rhizoctonia solani, fusarium graminearum, alternaria solani, phoma exigua var. horii and phoma sorghicola is the best, and the EC 50 Values are 0.258 μg / mL, 0.679 μg / mL, 0.424 μg / mL, 0.384 μg / mL and 0.975 μg / mL respectively; for the gossypium gossypium, the antibacterial effect of compound 12 is the best, and the EC 50 Value is 0.989 μg / mL; followed by compound 11, and the EC 50 Value is 1.248 μg / mL.The hydrazine compounds prepared with eugenol as lead have excellent, broad-spectrum and high-efficiency anti-plant pathogenic microorganism activity, and can be used for preventing and treating plant diseases caused by plant pathogenic microorganisms.

The application discloses a coumarin derivative, a preparation method and application thereof, and has a structure as shown in formula (I) and / or (II). The method for preparing the coumarin derivative is simple and efficient, and the application is that the prepared coumarin derivative has good in-vitro inhibitory activity on four common plant pathogenic fungi of Botrytis cinerea, Fusarium oxysporum, Alternaria alternata and Alternaria solani. The compound has good application in a plant-derived fungus inhibitor, is more environmentally friendly, and meets the green chemistry concept.

The invention belongs to the technical field of biological agents, and particularly relates to trichoderma harzianum, a biocontrol agent, a bio-organic fertilizer, a method for preventing and treating plant diseases and application. The invention provides a trichoderma harzianum P11 with the preservation number of CCTCC NO: M 2024648. The trichoderma harzianum P11 can improve the relative expression quantity of disease-resistant positive correlation genes PnCAD, PnSN1 and PnDEFL1 in plants and improve the activity of disease-resistant related enzyme activities SOD, POD and CAT, and the trichoderma harzianum P11 can damage cell membranes of pathogenic bacteria and inhibit the growth of the disease pathogenic bacteria. The trichoderma harzianum P11 has good rhizosphere colonization capability. The result of the embodiment shows that the trichoderma harzianum P11 can reduce the morbidity of panax notoginseng field root rot, and can also antagonize ginseng alternaria alternata, magnaporthe oryzae, polygonatum kingianum colletotrichum, geotrichum candidum, alternaria solani, cylindrosporium destroyed, phytophthora capsici and conidiospora spiraea.

The application discloses a bacillus velezensis BZWQ55, a microbial agent and application thereof, and belongs to the field of microorganisms. The bacillus velezensis BZWQ55 is screened from rhizosphere soil of potato scab and has a preservation number of CGMCC No. 36805. Experimental results show that the bacillus velezensis BZWQ55 has the abilities of producing cellulase, protease, amylase and iron carrier, and has the abilities of phosphorus and potassium solubilization and nitrogen fixation, and can effectively promote plant growth; the bacillus velezensis BZWQ55 can also stably inhibit the growth of Fusarium solani, Fusarium oxysporum, Alternaria solani, Streptomyces scabrisporus, Rhizoctonia solani and Neocosmospora vasinfecta, and has a significant prevention effect on multiple soil-borne diseases such as potato scab, sweet potato root rot, potato scab and peanut fruit rot. The application provides a new strain resource for biological control of plant soil-borne diseases and promotion of plant growth.

This invention discloses a transcription factor gene of Minjiang lily. LrbHLH1 Its nucleotide sequence is as described in SEQ ID NO: 1, encoding a protein with the amino acid sequence shown in SEQ ID NO: 2. This invention has confirmed this through reverse genetics technology. LrbHLH1 The gene has the function of enhancing plant resistance to fungi, and the present invention will... LrbHLH1 Genes were constructed into plant expression vectors and overexpressed in tobacco. The transgenic tobacco plants exhibited strong resistance to fungal infection, demonstrating the effectiveness of overexpression. LrbHLH1 The genetically modified tobacco plants exhibit high levels of resistance to infection by *Alternaria alternata* and *Alternaria solani*.

The invention discloses an application of Cu-NPs as a bacteriostatic agent of alternaria solani. The concentration of the Cu-NPs is 100 g * mL <-1 >. The Cu-NPs is low in production cost, safe and simple in application and operation method, experiments prove that the Cu-NPs have broad-spectrum inhibitory activity on plant pathogenic fungi, the antibacterial effect of the Cu-NPs at the concentration of 100 g.mL <-1 > is optimal, and the antibacterial effect of the Cu-NPs is superior to that of broad-spectrum bactericide thiophanate methyl. The antibacterial rate of the Cu-NPs solution with the concentration of 100 g.mL <-1 > on tomato early blight pathogenic bacteria alternaria solani reaches 85%.

The invention discloses bacillus velezensis SGTSH 1011, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No.34265. The invention further discloses a microbial pesticide preparation which comprises the bacillus velezensis SGTSH 1011 parent drug, a dispersing agent, an emulsifying agent, a stabilizing agent, a thickening agent and a dispersing medium. The invention also discloses application of the microbial pesticide preparation in prevention and treatment of sunflower sclerotiniose. The strain has remarkable inhibition activity on sunflower sclerotinia sclerotiorum pathogenic bacteria sclerotinia sclerotiorum, the inhibition rate reaches up to 85.68%, meanwhile, the strain shows a broad-spectrum antibacterial effect on various plant pathogenic fungi such as sorghum colletotrichum, fusarium oxysporum, fusarium graminearum and alternaria solani, and good biocontrol application potential is achieved. By optimizing a preparation formula, the technical problem that a living microbial preparation is easy to inactivate in the storage and transportation processes is solved.

The invention provides bacillus vallismortis TCS004 with a preservation number of CGMCC (China General Microbiological Culture Collection Center) NO.25850. The invention discloses an application of the bacillus vallismortis TCS004 in preventing and treating plant pathogenic fungi or plant pathogenic bacteria, or an application of the bacillus vallismortis TCS004 in preparing a microbial preparation for preventing and treating the plant pathogenic fungi or the plant pathogenic bacteria. The bacterial strain has a very high inhibition effect on plant pathogenic fungi such as lasiodiplodia mali, macrostem botryosphaeria verticillata, citrus interspersonia, streptosclerotinia sclerotiorum, alternaria alternata, colletotrichum frutescens, muscardine fungus, alternaria solani, fusarium solani, botrytis cinerea, fusarium equisetum, curvularia, alternaria alternata and the like; and the compound also has relatively good bacteriostatic activity on plant pathogenic bacteria xanthomonas oryzae and the like. The invention further provides a formula of a fermentation culture medium of the bacillus vallismortis TCS004 and fermentation process conditions of the bacillus vallismortis TCS004.

The invention discloses a coumarin derivative containing a Schiff base structure as well as a preparation method and application of the coumarin derivative in resisting plant pathogenic fungi, and belongs to the field of medicinal chemistry. The coumarin derivative containing the Schiff base structure is generated by taking coumarin as a parent and reacting with the Schiff base containing an azomethyl characteristic functional group, and is used for preventing plant fungal infectious diseases. The coumarin derivative containing the Schiff base structure, provided by the invention, has a good inhibition effect on botrytis cinerea, alternaria solani, fusarium oxysporum and alternaria alternata, and can be used as a lead compound for creating a novel agricultural bactericide.

The application discloses a benzopentaheterocyclic compound containing a hydrazine structure and an application thereof. The compound, stereoisomers of the compound, or a salt of the compound, or a solvate of the compound has good inhibiting effects on plant pathogenic bacteria and fungi. The compound has good inhibiting effects on fungi such as Colletotrichum gloeosporioides, Colletotrichum theae-sinensis, Mycosphaerella pachyrrhizi, Alternaria solani, Phytophthora capsici and the like, and pathogenic bacteria such as Xanthomonas oryzae, Xanthomonas citri and the like. Some of the compounds show excellent inhibiting activities on Colletotrichum theae-sinensis, with an EC 50 of 11.85 μg / mL. Some of the compounds show excellent inhibiting activities on Mycosphaerella pachyrrhizi, with an EC 50 of 2.88-16.41 μg / mL. Some of the compounds show excellent inhibiting activities on Xanthomonas citri, with a primary screening activity of 13.69-27.03 μg / mL, which is superior to a commercial drug, copper oxychloride. Some of the compounds show excellent inhibiting activities on Xanthomonas campestris, with a primary screening activity of 25.07-43.78 μg / mL, which is superior to the commercial drug, copper oxychloride. The application provides an important scientific basis for the research and development of new pesticides.

This invention discloses a class of imidazo[1,2-]azoline containing a hydrazone structure. a Preparation methods and applications of pyridine derivatives, this invention uses imidazo[1,2- a Using the pyridine skeleton as the parent structure, a series of imidazo[1,2-] hydrazone fragments were introduced to design and synthesize a series of imidazo[1,2-] hydrazone-containing structures. a Pyridine derivatives. In vitro activity results showed that these compounds had excellent inhibitory activity against plant pathogenic fungi (such as *Botrytis cinerea*, *Colletotrichum gloeosporioides*, *Colletotrichum sorghum*, *Colletotrichum tea*, *Alternaria alternata*, and *Rhizoctonia solani*), and some of the target compounds also showed good inhibitory activity against plant pathogenic bacteria.

The application relates to a microbial compound bacterium H1 antagonizing multiple potato pathogenic fungi and an application and a preparation method thereof, and relates to the field of biological bacterial agents. The microbial compound bacterium H1 is mixed by bacillus subtilis, bacillus atrophaeus and bacillus cereus. The microbial compound bacterium H1 is used for preparing a bacterium agent antagonizing potato pathogenic fungi. The H1 has a strong inhibiting effect on the early blight pathogenic fungi alternaria solani and alternaria alternata, the dry rot pathogenic fungi fusarium solani, fusarium oxysporum and fusarium equiseti, and the leaf / stem spot pathogenic fungi botryodiplodia theobromae, and the inhibiting rates are all higher than 45%. The inhibiting rate on the botryodiplodia theobromae is the highest, reaching 76.92%. The H1 provides a high-quality strain for comprehensively preventing and treating potato soil-borne diseases, and has a good field application prospect.

The invention discloses a chalcone compound, and particularly discloses a structure of the compound. The invention also provides a preparation method and application thereof. A biological activity test result shows that the compound has relatively good inhibitory activity on six plant fungal diseases including lycium barbarum root rot bacteria, apple alternaria leaf spot bacteria, botrytis cinerea, celery leaf spot bacteria, valsa mali and potato black scurf bacteria, and the inhibitory activity of part of the compound on the bacteria is superior to that of azoxystrobin; the compound has a prospect of becoming a novel bactericide.

The invention discloses an enterobacter cancerogenic EcCB2 strain and an application thereof. According to the present invention, the enterobacter cancerogenus EcCB2 strain is separated from lasioderma serricorne, and is preserved in Guangdong Province Microbial Culture Collection Center on May 31, 2024, and the preservation number is GDMCC No: 64709; the EcCB2 strain not only can effectively kill vegetable pests such as beet armyworms, spodoptera litura, prodenia litura, plutella xylostella and cabbage caterpillars which are mainly harmful to brassicaceous vegetables, but also can remarkably inhibit the growth of plant pathogenic fungi such as fusarium oxysporum, sclerotinia sclerotiorum, colletotrichum hijinensis and alternaria solani; the compound can be used for preventing and treating plant diseases caused by plant pathogenic fungi and cruciferous vegetable insect pests.

The application discloses pyridine coumarin derivatives, a preparation method and application thereof. The pyridine coumarin derivative is selected from the following structural general formula: the pyridine coumarin derivative is independently designed and synthesized, and the experimental results show that the pyridine coumarin derivative has good antifungal activity on botrytis cinerea, alternaria solani, fusarium oxysporum and alternaria alternata, which expands a new field for prevention and treatment of various plant fungal diseases, has wide development space and good development and application prospect, and has good application value.

The invention discloses a novel meroterpenoid compound as well as a preparation method and application thereof. The structural formula of the compound is shown as a formula I. The compound is separated from an Alternaria sp. XZ146 solid fermentation extract for the first time, and the antiviral activity of the compound is reported for the first time. Experimental results show that the compound in the formula I can be used for preparing an antiviral preparation, especially an anti-influenza A preparation.

The application discloses a strain of Pantoea dispersa for preventing and treating Chinese rose gray mold and prolonging the flowering period of Chinese rose, and application thereof. The strain is Pantoea dispersa SJJ17CGMCC NO.30658. The strain can antagonize plant pathogens such as Colletotrichum gloeosporioides, Alternaria solani, Fusarium proliferatum and Fusarium oxysporum. The antagonistic substance produced by the strain has certain resistance to high temperature and ultraviolet rays. The strain can also produce siderophores and has the characteristics of dissolving inorganic phosphorus. The application provides an excellent biocontrol strain for preventing and treating Chinese rose gray mold, and has great production significance for green prevention and control technology of Chinese rose diseases and sustainable development of the Chinese rose industry.

The invention discloses a substituted quinoline compound and a preparation method and application thereof, the substituted quinoline compound with bactericidal activity and herbicidal activity is successfully synthesized by a multi-step reaction method taking THF as a solvent, sodium hydride as a reducing agent and polyphosphoric acid as a cyclizing agent, the synthesized compound is confirmed by characterization means such as NMR (nuclear magnetic resonance) and the like, and the substituted quinoline compound has a good application prospect. 16 target compounds are subjected to bactericidal activity and herbicidal activity tests. Experimental results show that part of the compounds have a remarkable inhibiting effect on various phytopathogens, and particularly have a good sterilizing effect on alternaria solani, magnaporthe oryzae and fusarium graminearum at the concentration of 50 ppm. In addition, part of the compounds also have a certain weeding effect on dicotyledonous plants such as oilseed rape, and a new pesticide development direction is provided. The invention has a wide application prospect, and especially has important potential in agricultural pest control.

The present application belongs to the technical field of agricultural compositions, and particularly relates to a composition containing amicarbalide and copper sulfate, a pesticide preparation and application thereof. The active ingredient of the composition is amicarbalide and copper sulfate, and the mass ratio of the two is 7-9:10-19. Under the specific ratio, the two show a significant synergistic control effect on tomato early blight caused by Alternaria solani. The present application also provides a stable pesticide preparation containing the active ingredient, which contains disodium ethylenediaminetetraacetate, an antifreezing agent, a thickening agent, a surfactant, an acid-base regulator and water. The preparation method of first complexing copper sulfate with disodium ethylenediaminetetraacetate to form a mother liquor effectively solves the stability problem of directly mixing amicarbalide with copper ions. Potting and field tests show that the control effect of the pesticide preparation of the present application on tomato early blight is significantly better than that of each single agent, and a new scheme of high efficiency and low resistance risk is provided for the control of tomato early blight.

This invention discloses a multifunctional stem-coating medicated fertilizer solution, its preparation method, and its application, belonging to the field of bio-organic fertilizer technology. The multifunctional stem-coating medicated fertilizer solution of this invention comprises the following raw materials: wood vinegar, microbial inoculants, earthworm protein peptides, *Alternaria solani* extract, potassium humate, lecithin, sodium carboxymethyl cellulose, chitin, urea, potassium dihydrogen phosphate, a chelated trace element mixture, and water. The microbial inoculants include *Bacillus laterosporus*, *Burkholderia ulmoides*, and *Trichoderma pleurotus*. This invention, through the scientific compounding of three specific strains, works synergistically with the other raw materials in the formula, solving the problems of limited disease resistance and low fertilizer utilization rates in existing apple tree stem-coating agents. While effectively controlling apple tree rot, it also significantly promotes fruit enlargement, increases yield and quality, improves tree vigor, and is environmentally friendly, with no adverse effects on the environment or fruit, showing broad application prospects.

This invention discloses a Cyclophilin antibacterial protein gene from the Minjiang lily. LrCYP1 Its nucleotide sequence is as described in SEQ ID NO: 1, encoding a protein with the amino acid sequence shown in SEQ ID NO: 2. This invention confirms through functional genomics-related research. LrCYP1 The gene has the function of enhancing plant antifungal resistance, and the antifungal properties of this invention... LrCYP1 Genes were constructed into plant expression vectors and overexpressed in tobacco. The transgenic tobacco plants exhibited strong resistance to fungal infection, demonstrating the effectiveness of overexpression. LrCYP1 The genetically modified tobacco plants exhibit high levels of resistance to Alternaria alternata and Pseudomonas spp.

The application discloses a chalcone isomerase gene of Lilium regale LrCHI1 The nucleotide sequence is shown as SEQ ID NO:1, and the protein encoded by the amino acid sequence shown as SEQ ID NO:2. LrCHI1 The application proves that the gene has the function of improving the plant resistance to fungi through reverse genetics technology research. LrCHI1 The gene is constructed into a plant expression vector and is introduced into tobacco to be overexpressed, and the transgenic tobacco plant has strong anti-invasion ability to fungi, and experimental results show that the transgenic tobacco with overexpression has high-level resistance to the invasion of Phoma exigua and Alternaria solani. LrCHI1 ​