104 results about "Alternaria solani" patented technology

The invention discloses a preparation method and applications of wild jujube endophytic bacillus amyloliquefaciens new strain SZ23 and fermentation broth. The bacillus amyloliquefaciens new strain SZ23 is preserved at China General Microbiological Culture Collection Center on July, 25th, 2014, and preservation number is CGMCC No.9481. Morphological characteristics, physiological and biochemical indexes, and 16S rDNA sequencing results of bacillus amyloliquefaciens new strain SZ23 are detailedly disclosed by the invention. It is shown by bacteriostasis experiments that, the fermentation broth of bacillus amyloliquefaciens new strain SZ23 possesses excellent bacteriostatic activity on alternaria kikuchiana, fusarium oxyspirum F sp niveum, fusarium oxysporum Schl.f.sp vasinfectum(Atk.)Snyd.& Hans, alternaria solani, fusarium wilt of cucumber, cotton rhizoctonia solani kuhn, fusarium graminearum schw, and botrytis cirerea Pers et Tris; bacteriostasis rate ranges from 55.6% to 92.2%; and it is confirmed that wild jujube endophytic bacillus amyloliquefaciens new strain SZ23 possesses excellent broad-spectrum antibacterial activity. It is confirmed by practical experiments that control rate of the fermentation broth of new strain SZ23 on alternaria kikuchiana is more than 75%.

The invention discloses a bacillus velezensis strain CSQXDZ26, which is bacillus velezensis (Bacillus velezensis) with the preservation number of CGMCC NO. 17935. The bacillus velezensis CSQXDZ26 hasremarkable antagonistic activity on bacterial plant diseases such as Xanthomonas oryzae pv. Oryzae, Xanthomonas oryzae pv. oryzicola, and xanthomonasaxonopodis pv. glycines AND THE LIKE; meanwhile, the bacillus velezensis CSQXDZ26 also has antibacterial activity on fungal plant diseases such as dithiorella gregaria, Fusarium graminearum, Fusarium oxysporum f.sp.Cucumerinum, Fusarium oxysporum f.sp. momordicae, Alternaria solani or Alternaria alternate, and also has excellent inhibition effect on common food spoilage bacteria such as bacillus subtilis, proteusbacillus vulgaris and staphylococcus aureus.

The invention discloses a bacillus atrophaeus strain DPPG-28 and an application in crop disease control, and belongs to the field of agricultural biotechnology. The strain is separated from soil of drip irrigation fields with damping off in tomato processing production areas, and has an accession number of CGMCC No. 9394. The strain can be used for biological control of crop pathogens such as rhizoctonia solani, pythium aphanidermatum, alternaria solani, phytophthora capsici, tomato scab fungi, and the like; the control effects on tomato damping off, capsicum early blight, and cotton damping off reach respectively 74.17%, 66.83%, and 70.12%; the strain can significantly improve the crop germination rate, plant height, fresh weight, and dry weight at the seedling stage. The strain of the invention has high-efficient wide-spectrum germicidal activity, and has wide application prospects in biocontrol agents.

The invention discloses aspergillus tubingensis with disease prevention and growth promoting functions as well as preparation and an application of aspergillus tubingensis metabolites. The bacterial strain No. of aspergillus tubingensis is QF05, and the registration No. of aspergillus tubingensis in CGMCC (China general microbiological culture collection center) is CGMCC No.13563. Aspergillus tubingensis QF05 has an inhibition function on botrytis cinerea, colletotrichum capsici, fusarium wilt of cucumbers, alternaria solani and pseudomonas syringae pv.lachrymans. Sterile fermentation filtrate of aspergillus tubingensis QF05 can remarkably increase the germination rate of pepper seeds and promote elongation of radicle. Aspergillus tubingensis QF05 metabolites containing bacteria effectively promote growth of true leaves of cucumbers and effectively increase the plant height and the yield of the cucumbers.

The invention relates to a polygonum aviculare botanical bactericide and a preparation method thereof, belonging to the technical field of pesticides. The invention aims at disclosing a compound botanical bactericide formula and a preparation method thereof. The polygonum aviculare botanical bactericide is mainly prepared from polygonum aviculare, radix scutellariae, black nightshade herb, radix gentianae, rhizoma atractylodis and the like. The preparation method comprises the steps of 1, drying and crushing the raw materials, then sieving and mixing the raw materials; 2, performing ultrasonic treatment on the mixture after being subjected to cold soak in ethanol, filtering to obtain a filtered liquid, evaporating and condensing until the filtered liquid becomes paste. The polygonum aviculare botanical bactericide has a relatively good bacteriostasis function on multiple pathogenic fungal plant pathogens such as rhizoctonia solani, pythium blight pathogen, fusarium graminearum, alternaria solani and carbon-color helminthosporium carposaprum, has the effects obviously better than the chemical disinfectant thiram. The raw materials adopted in the invention are purely natural plants, so that the preparation cost is low, and small-size industrial production can be carried out; furthermore, as the plants are Chinese herbal medicines, the polygonum aviculare botanical bactericide can be degraded rapidly after the use, is safe for people and live stock, good in environmental compatibility and free from secondary pollution to the environment, and meets the current concept of green food and environment sustainable development strategy.

The invention provides a Bacillus mojavensis QLY002 strain. The Bacillus mojavensis QLY002 strain has a preservation number of CGMCC No. 8905. The invention also provides a microbial inoculum of the Bacillus mojavensis QLY002 strain and its preparation method and use in potato pathogen inhibition and potato growth promotion. The Bacillus mojavensis QLY002 strain and the microbial inoculum can be used as microbe pesticides and fertilizer, can inhibit Phoma foveata, Alternaria solani, Fusarium oxysporum and Colletotrichum coccodes, have an antibacterial rate more than 61%, have potato growth promotion effects, can be used as potato special-purpose microbial pesticide and microbial fertilizer, solve the problem that the existing chemical pesticide has poor environmental friendliness, easily causes pesticide resistance and has poor safety, and have the advantages of good environmental friendliness, no pesticide resistance and good safety.

The invention discloses application of sirolimus in inhibition of plant alternaria solani and further discloses a fungicide. The fungicide contains rapamycin or a mixture of the rapamycin and a chemical fungicide. A TOR protein inhibitor rapamycin used in clinical medicine is used for prevention and control research of plant early blight for the first time, it is found through the research that the rapamycin and the mixture of the rapamycin and the chemical fungicide can effectively inhibit the plant alternaria solani, a chemical rapamycin pesticide can play a synergistic effect to inhibit the plant alternaria solani, a very significative base is provided for research of a novel drug for preventing and controlling the early blight, the novel fungicide is wide in application prospect and market prospect, a new choice is provided for early blight prevention and control, the using amount of the chemical pesticide can be remarkably decreased, and the fungicide has the great significance on reduction of pesticide usage amount and delay of the resistance of insect pests and also has the great application value on maintenance of ecosystem balance and sustainable development.

The invention belongs to a method for preparing inula japonica plant extract and applications of the inula japonica extract. The plant material (root, stem, leaves, inflorescence and whole grass) of the inula japonica (including Inula japonica and Inula britannica) is crushed and extracted by a solvent which comprises 95% of alcohol; and the extract solution is concentrated to have the same weight with the inula japonica crushed matters, thus obtaining inula japonica extract. The inula japonica extract has obvious inhibition effects to cucumber botrytis cinerea, alternaria solani, cladosporium fulvum, colletotrichum lagenarium, sphaerotheca fuliginea, pseudoperonospora cubensis, potato phytophthora infestans, tomato phytophthora infestans and rhizoctonia cerialis; wherein the inflorescence extract has strong antibacterial activity. The inula japonica extract extracted by alcohol can be matched with suitable solvent and pesticide adjuvant to prepare britannica lactone emulsifiable solution and britannica lactione micro-emulsion of botanical fungicide. The inula japonica extract has obvious effects for preventing cucumber downy mildew, sphaerotheca fuliginea, tomato gray mold and tomato leaf mould, is especially suitable for prevention and control on the fungi diseases to economical crops such as vegetables and the like, and is a botanical fungicide with nuisanceless.

The invention discloses a plant growth-promoting rhizobacteria strain which is separated from dung of bos grunniens in Qinghai Province and preserved in the General Microorganisms Collection Center of China Committee for Culture Collection of Microorganisms with a preservation name of Bacillus subtilis QM34 and a preservation number of CGMCC No. 2669 on September 22, 2008; the strain QM34 has antagonistic action on 12 pathogenic bacteria of soil-born plant fungus; and the fermentation solution of the strain QM34 and the cell-free nutrient solution of the fermentation solution of the strain QM34 has obvious prevention and treatment effects on alternaria solani, significantly promotes the growth of tomato plants and can be colonized in a large quantity according to the tomato plant rhizosphere. Therefore, the rhizobacteria strain is a broad-spectrum growth-promoting rhizobacteria strain used or preventing and treating alternaria solani and most popular among people.

The invention discloses a multifunctional insecticide fertilizer for tomatoes and a preparation method of the multifunctional insecticide fertilizer. The multifunctional insecticide fertilizer is prepared from the following raw materials in parts by weight: 31-31.62 parts of furfural residue, 13-13.4 parts of urea, 21-21.6 parts of potassium sulfate, 23-23.5 parts of monoammonium phosphate, 1.8-2 parts of zinc sulfate, 1.8-2 parts of polyvinyl alcohol, 1.8-2 parts of polyacrylamide, 2.1-3.2 parts of bactericide, 0.2-0.4 part of citric acid, 0.06-0.08 part of compound sodium nitrophenolate and 0.16-0.2 part of EDTA. The multifunctional insecticide fertilizer for tomatoes disclosed by the invention is reasonably and especially designed according to the plentiful-lack indexes of nutrients of the soil in Hexi corridor in Gansu Province, and the absorption ratio of the tomatoes on nutrient elements; a series of problems that a greenhouse tomato field in Hexi corridor in Gansu Province is unbalanced in ratio of macroelements to microelements, low in organic matter content, and poor in water retaining capacity, and generates soil hardening, alternaria solani, late blight, gray mold, viral disease, bacterial wilt, septoria lycopersici and the like are solved, and the multifunctional insecticide fertilizer integrates nutrition, disease prevention, soil improvement and water retention.

The invention discloses an alpha-amino phosphonate compound with a 2-amino-1, 3, 4-thiadiazole structure and a preparation method and application of alpha-amino phosphonate compound. The alpha-amino phosphonate compound has the sterilizing effect of alpha-amino phosphonate compounds and has a pesticide effect by introducing the 2-amino-1, 3, 4-thiadiazole group, and the alpha-amino phosphonate compound is wide in application range and good in sterilizing and pesticide effect by combining the alpha-amino phosphonate compound with the 2-amino-1, 3, 4-thiadiazole group. The preparation method is few in step, simple to operate, high in yield and productivity, capable of saving a large amount of time and cost in actual production and good in economic benefit. The 2-amino-1, 3, 4-thiadiazole has an inhibition effect on the growth and proliferation of alternaria solani and on the growth of mythimna separata and can be used as the pesticide intermediate to apply to the preparation of different agricultural fungicide.

The invention provides an alternaria solani PCR detection specific primer which comprises an upstream primer ASF: 5'-ACCACAAGGACCAACCCATA-3' and a downstream primer ASR: 5'-CCTACCTGATCCGAGGTCAA-3'. The invention further provides an alternaria solani PCR rapid detection method by using the primer, according to the method, a to-be-tested sample genome is taken as a template, the primer is used for PCR amplification, and a 409 bp amplified fragment can be detected by using gel electrophoresis after amplification is finished. The primer has high specificity, the sensitivity is high, the operation process is easy and convenient to operate, the detection requirement for alternaria solani on agricultural production can be met, and the defects that the operation is tedious, consumed time is long, the accuracy is low and the like in a conventional detection method are overcome.

The invention discloses a 4-trifluoromethyl-7-hydroxycoumarin derivative as well as a preparation method and application thereof. The yield of the preparation method is high. The invention also discloses application of the 4-trifluoromethyl-7-hydroxycoumarin derivative to an agricultural anti-fungal aspect. An inventor detects the toxicity effect on rice sheath blight disease, colletotrichum lagenarium, fusarium graminearum, strawberry botrytis cinerea, apple spot germs and alternaria solani by a growth rate method; certain lateral reactivity is realized on the 4-trifluoromethyl-7-hydroxycoumarin derivative; the activity of individual compounds is superior to that of contrast medicine; the effect of the compound in the aspect of inhibiting the rice sheath blight disease is prominent; or the compound can become a lead compound molecule for developing and creating new pesticide.

The invention belongs to the technical field of kind and molecule identification of plant pathogenic fungi, and aims to provide a detection kit for simply, rapidly and accurately identifying Alternaria solani Sorauer in potato blade tissues based on a polymerase chain reaction (PCR), and a detection method thereof. The detection kit comprises a pair of self-designed specific PCR primers and Mix, and the sequences of the specific primers comprise AsF:5'-CCCGCAAGGGGAGACAAA-3' and AsR:5'-CACCTCCCGGGGTGGCCA-3'. The detection method comprises the following steps: extracting blade or mycelium DNA, and carrying out PCR amplification by using the specific primer pair comprising AsF and AsF; carrying out agarose gel electrophoresis to analyze whether a 358bp strip specifically appears or not; and determining that whether a tested sample is Alternaria solani Sorauer according to the determined result of the above amplification strip. The method effectively solves the problems of Alternaria solani Sorauer field early-stage diagnosis and difficult kind identification caused by no spore production of isolates, realizes the early-stage diagnosis and detection of early blight, and provides technical support for scientific research people to molecularly identify the Alternaria solani isolates not producing spores.

The invention discloses applications of natural compound Murrayone as an agricultural bactericide. Natural compound Murrayone is taken as an agricultural bactericide used for preventing and treating agricultural fungal diseases, is capable of preventing and treating Sclerotinia sclerotiorum, Thanatephorus cucumeris, Botrytis cinerea, Fusarium graminearum, Colletotrichum gloesporioides, and Alternaria solani effectively, and possesses excellent treatment effects on fungal diseases such as Sclerotinia sclerotiorum, Thanatephorus cucumeris, and Colletotrichum gloesporioides. Agricultural bactericides prepared by taking natural compound Murrayone as an active ingredient possesses excellent curative effects, broad spectrum activity, and low toxicity, and are especially beneficial for environmental protection; no residue is left; and no pollution is caused.

The invention relates to the field of microbial technology and especially relates to a use of Streptomyces sioyaensis metabolite in prevention and control of Colletotrichum lindemuthianum. Llex cornuta endophyte separated from the llex cornuta living bodies in Hangzhou city of Zhejiang province by an endophyte microorganism separation technology is identified as Streptomyces sioyaensis by microbial taxonomy and is named as Streptomyces sioyaensis 107. The Streptomyces sioyaensis 107 is preserved in the China general microbiological culture collection center on December 12, 2012 and has a preservation number of CGMCC No. 6984. Through liquid fermentation, the Streptomyces sioyaensis 107 can produce active metabolites having effects of inhibiting plant fungal disease pathogenic bacteria such as Colletotrichum lindemuthianum and Alternaria solani and is an important microbe resource in agriculture.

The invention discloses a strain of streptomyces hydrogenans and application thereof in biological control. The preservation number of the streptomyces hydrogenans is CGMCC No. 17449. The applicationof the streptomyces hydrogenans specifically refers to application in control over alternaria solani, and includes the steps that streptomyces hydrogenans thalli are activated, fermentation culture iscarried out until the thalli grow to the logarithmic phase, fermentation is stopped, and the obtained fermentation product is a biocontrol agent; the obtained biocontrol agent is diluted, and root dipping is carried out with the diluent when plant seedlings are transplanted and/or root irrigation is carried out with the diluent when seedlings grow to the 4-5 leaf stage. The application of the streptomyces hydrogenans can replace chemical pesticides to effectively control alternaria solani diseases. The biocontrol agent contains growth-promoting components and beneficial microorganisms, and can effectively protect and promote the root growth and enhance the resistance of the root system to adverse environments and harmful bacteria; the agent also contains components capable of inhibiting and killing alternaria solani and can effectively control plant fungal diseases caused by alternaria solani.

The invention discloses a group of microsatellite primers for diversity analysis of early blight pathogen alternaria solani, and provides a method for performing diversity and population genetic structure analysis by using the group of primers. The group of primers is screened and designed from a genome library. The group of primers is stable in PCR (polymerase chain reaction) amplification products and favorable in polymorphism, and can be used for diversity and population genetic structure analysis.

The invention provides loop-mediated isothermal amplification primers for detecting Solanum lycopersicum L Alternaria solani, and applications thereof, wherein the primers comprise four specific primers F3, B3, FIP and BIP4, and the nucleotide sequences represented by SEQ ID NO.1-4. According to the present invention, the problems of long period, time consumption, labor consumption, complexity, poor specificity, and requirement of thermal cycle instrument and other expensive equipment by PCR detection technology in the detection method for Solanum lycopersicum L Alternaria solani in the priorart can be solved with the primers; and the detection system of the present invention can rapidly, efficiently, highly-specific and highly-sensitive detect Solanum lycopersicum L Alternaria solani under the LAMP amplification condition, is suitable for the on-site detection of pathogenic bacteria and diseases, is suitable for large-range promotion and application, and can provide the reliable technical and theoretical basis for the prevention and treatment of Solanum lycopersicum L early blight.

The invention relates to the field of microbial technology and especially relates to a use of Streptomyces sioyaensis metabolite in prevention and control of Alternaria solani. Llex cornuta endophyte separated from the llex cornuta living bodies in Hangzhou of Zhejiang province by an endophyte microorganism separation technology is identified as Streptomyces sioyaensis by microbial taxonomy and is named as Streptomyces sioyaensis 107. The Streptomyces sioyaensis 107 is preserved in the China general microbiological culture collection center on December 12, 2012 and has a preservation number of CGMCC No. 6984. Through liquid fermentation, the Streptomyces sioyaensis 107 can produce active metabolites having effects of inhibiting plant fungal disease pathogenic bacteria such as Colletotrichum lindemuthianum and Alternaria solani and is an important microbe resource in agriculture.

The invention belongs to the field of pesticides and discloses a benzimidazolyl containing amide derivative, a preparation method therefor and an application of the benzimidazolyl containing amide derivative. The derivative has a structure represented by a formula (I) shown in the description. The compound disclosed by the invention is simple and convenient in preparation method, has a relativelygood inhibiting action on Gibberella zeae, Botrytis cinerea, Rhizoctonia solani and Alternaria solani and can be applied to control on fungal diseases of plants.