14 results about "Xanthomonas campestris" patented technology

The present application relates to a kind of parabacillus (Bacillus paralicheniformis) Bacillus paralicheniformis ) HMF14, the preservation number is CGMCC No.30480.The parabacillus HMF14 of the present application can resist salt and alkali, can simultaneously antagonize fusarium oxysporum, microdochium nivale var. avenae and xanthomonas campestris pv. tridii, also has toxic effect to pratylenchus penetrans and tetranychus cinnabarinus, can effectively prevent and treat wheat take-all disease and wheat black foot disease.

This invention belongs to the field of genetic engineering and biotechnology, specifically relating to a β-glucosidase PO3 for the conversion of rare ginsenosides, its preparation method, and its applications. This invention obtains β-glucosidase PO3 by PCR amplification using genomic DNA of *Xanthomonas pseudoepiplo* TY3-10 as a template. The results of the examples show that this enzyme can not only convert total ginsenosides into rare ginsenosides F2, C-O, C-Mx1, and C-Mc1, but also convert ginsenoside Rb1 into F2, ginsenoside Rb2 into C-O, ginsenoside Rb3 into C-Mx1, and ginsenoside Rc into C-Mc1; it also exhibits excellent catalytic performance and high expression efficiency, possessing extremely high application value and commercial prospects.

This invention discloses a metal ion-driven meliosteum peptide nanoassembly, its preparation method, and its applications, relating to the field of agricultural biotechnology. The meliosteum peptide nanoassembly is formed by the self-assembly of a hexahistidine-tagged meliosteum peptide with divalent metal ions through coordination. The average particle size of the meliosteum peptide nanoassembly is 20 nm–30 nm. The meliosteum peptide nanoassembly obtained by this invention through the self-assembly of a hexahistidine-tagged meliosteum peptide with zinc ions exhibits excellent antibacterial, anti-biofilm activity, stability, and biocompatibility, and can be used to control plant bacterial diseases caused by Xanthomonas and other fungi.

The invention discloses construction and application of an engineering strain capable of normally growing at 35 DEG C and realizing high yield of xanthan gum. The method comprises the following steps: by taking xanthomonas campestris XC1 as an initial strain, carrying out ARTP mutagenesis treatment, and screening a mutant strain which can normally grow under the condition of 35 DEG C; the mutant strain is subjected to multiple rounds of superposition transformation, and multiple physiological restrictions under high temperature stress are systematically broken by sequentially strengthening a protein folding system (grpE-ATGA-dnaK), activating outer membrane stress regulation (rpoE), constructing an anti-oxidation barrier (katB) and strengthening a cell membrane structure (polCDE); the finally obtained engineering strain shows the performance far better than that of an initial strain under the condition of 35 DEG C; the biomass of the strain is increased by 207.7% compared with that of an initial strain XC1, and the yield of xanthan gum is increased by 75.5% and reaches 25.8 g / L, which is consistent with the yield of xanthan gum under the fermentation condition of 28 DEG C.

The application discloses a polypeptide for presenting a heterologous protein to an outer membrane vesicle and application thereof. Specifically disclosed are a polypeptide with an amino acid sequence of SEQ ID No. 1 and application thereof in presenting a heterologous protein to an outer membrane vesicle of a host bacterium. The application successfully expresses the heterologous protein in OMVs of Xanthomonas campestris by connecting the polypeptide with the heterologous protein. Xanthomonas campestris is a plant pathogenic bacterium, has low immunogenicity to human bodies, has a unique advantage for biomedical application of OMVs, and realizes presentation of the heterologous protein in Xanthomonas campestris OMVs for the first time, thereby providing a solution for future plant biotechnology, development and application of a vaccine adjuvant and the like. The polypeptide of the application realizes presentation of the heterologous protein by using only 25 amino acids, and can minimally affect the conformation and activity of the presented protein.

本发明提供了蛋白突变体、包含该蛋白突变体的黄原胶生产菌株及应用。该黄原胶生产菌株包括一株高产黄原胶的黄单胞菌(Xanthomonas campestris)，菌株命名为黄单胞菌HXC001a，保藏于中国微生物菌种保藏管理委员会普通微生物中心，保藏编号为CGMCC No.38053。该菌株的基因组发生突变，导致编码的Q8P6W9蛋白存在T1158P突变。发酵实验表明，该菌株的黄原胶产量可达38.6 g / L，较出发菌株提高36.9%以上，且该高产表型具有良好的遗传稳定性。本发明提供的黄单胞菌可显著提高黄原胶产量，适用于工业化生产黄原胶，具有极高的应用价值。

The application discloses a Paraconiothyrium cyclothyrioides PF432 and a preparation method and application of metabolites thereof. The strain is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC M 20252943. Through liquid fermentation and chromatographic separation, 32 compounds, i.e. compounds 1-21, 22-29 and 30-32, are obtained from fermentation products of ME culture medium, PD culture medium and Gao's No. 1 culture medium of the strain, and the structures are identified through NMR, MS and single crystal X-ray diffraction analysis. Active tests show that the compounds have significant inhibiting effects on Xanthomonas oryzae pv. oryzae and Xanthomonas campestris pv. oryzae, wherein the in-vivo prevention and treatment effects of compounds 10, 15 and 16 on Xanthomonas oryzae pv. oryzae at a concentration of 500 ng / mL are better than that of positive control milbemycins. The application provides new candidate compounds for green prevention and control of plant bacterial diseases.

The application discloses a strain of Cladosporium oxysporum P1 and application of the strain in degrading polybutylene adipate terephthalate plastic, and the strain is named as Cladosporium oxysporum in classification. (Cladosporium oxysporum ), and the strain is named as P1, has been preserved in the China Center for Type Culture Collection, and the strain preservation number is CCTCC M 2026159, and the preservation date is January 16, 2026. The Cladosporium oxysporum P1 used in the application is obtained by separation and purification in farmland soil, can grow and degrade PBAT containing a benzene ring structure under normal temperature conditions in a PDB flask, and the degradation rate of the Cladosporium oxysporum P1 to PBAT mulch reaches 88.41% within 9 days. The Cladosporium oxysporum P1 can efficiently degrade PBAT containing a benzene ring aromatic structure, and overcomes the technical difficulty that polyester containing an aromatic ring is difficult to biodegrade.

This invention discloses a sugarcane white streak disease resistance gene. ShPR10 The cloning and application of its promoters belong to the field of bioengineering technology. ShPR10 The nucleotide sequence of the gene is shown in SEQ ID NO: 1, and the amino acid sequence of the protein it encodes is shown in SEQ ID NO: 2. This invention discovers... ShPR10 The gene can respond to Xanthomonas aureus infection stress, during Xanthomonas aureus infection. ShPR10 Gene expression levels increased significantly. Further investigation using a transgenic Arabidopsis system confirmed this. ShPR10 Genes can positively regulate plant disease resistance, that is, by overexpressing genes in plants. ShPR10 ShPR10 ShPR10 ShPR10 ShPR10 ShPR10 ShPR10 ShPR This invention aims to improve plant resistance to pathogenic tomato species of *Pseudomonas syringae*. It provides a theoretical basis for elucidating the stress response mechanism of sugarcane to *Xanthomonas syringae* infection and also offers valuable genetic resources for disease-resistant sugarcane breeding.

This invention discloses a method for preparing and genetically transforming *Cyclophorus fragrans* protoplasts. The genetic transformation method includes the following steps: A1. Adding STC buffer to *Cyclophorus fragrans* protoplasts and adjusting the concentration to obtain a protoplast solution; A2. Mixing exogenous plasmid DNA containing the target gene with the protoplast solution, adding PTC buffer, and allowing it to stand at room temperature to allow the plasmid DNA to integrate into the *Cyclophorus fragrans* genome; A3. Adding TB3 liquid medium for regeneration culture; A4. Adding first transformant selection medium and second transformant selection medium to the regeneration culture mixture, respectively, and culturing under dark conditions to obtain *Cyclophorus fragrans* transformants. This invention, by optimizing the enzymatic digestion system and transformation and screening conditions, achieves a protoplast yield of 3.84 × 10⁻⁶. 6 With a transformation efficiency of 32 transformants / μg DNA, and being simple to operate and highly reproducible, this method provides key technical support for molecular biological research on *Ceratophyllum demersum*.

This invention relates to the field of biodegradation, specifically to a liquefied Serratia marcescens and its application in the degradation of atrazine. The liquefied Serratia marcescens was deposited on January 12, 2026, at the China General Microbiological Culture Collection Center (CGMCC), with accession number CGMCC No. 37176. This invention discloses the utilization of... Serratia liquefaciens A1 degrades atrazine. This bacterium exhibits a degradation rate of up to 99.19% for atrazine in water and up to 98.50% for atrazine in soil. The strain of this invention demonstrates strong biodegradation ability for atrazine, with rapid degradation and high efficiency. It can be used to prepare formulations for degrading atrazine or for remediating atrazine pollution in the environment. The strain provided by this invention enriches the strain resource library of atrazine-degrading bacteria, offering a new option for the degradation of triazine herbicides and the bioremediation of polluted environments.

Provided herein is a method of producing xanthan gum from ዉ local isolate of Xanthomonas campestris ርርቪ1. It provides a non-cloned strain, namely Xanthomonas Campestris cdt1, which [5 suitable for efficient fermentation production in the lab and large-scale xanthan gum production. According to the invention, by isolating Xanthomonas Campestris ርርቪ1 and the application method thereof disclosed by the invention, xanthan gum can ከ6 efficiently fermented and produced via steps that may include activation culturing liquid seed culturing,fermentation culturing, and xanthan gum precipitating. The strain disclosed by the invention can be used for efficiently fermenting and producing xanthan gum by taking sucrose and glucose as carbon sources and peptone, yeast extract, ammonium sulfate, and ammonium chloride as nitrogen sources

The application discloses a Pseudomonas sp. and application thereof in degradation of nicotine, and the strain is Pseudomonas sp. J40, which is classified and named as Pseudoxanthomonas Pseudomonas sp. and is preserved in the China General Microbiological Culture Collection Center on November 20, 2025, with a biological preservation number of CGMCC NO. 36700. The Pseudomonas sp. J40 is obtained through strain activation, seed liquid preparation and fermentation liquid preparation, and is applied to fermentation of cigar tobacco leaves, so that the nicotine content of the cigar tobacco leaves can be effectively reduced, the quality of the cigar tobacco leaves, especially the domestic cigar tobacco leaves, can be improved, the process is simple, and the reaction condition is mild. After the fermentation treatment, the strength and the peculiar smell of the domestic cigar tobacco leaves are obviously reduced, the tobacco smoke is soft and delicate, the irritability is weakened, and the aftertaste is clean.

The application discloses a SNP marker and a KASP primer group for detecting the resistance of tobacco wild fire bacteria No. 0 physiological race and application thereof. The application discloses a SNP marker for detecting the resistance of tobacco wild fire bacteria No. 0 physiological race, wherein the SNP marker is located at the position of 111102247 of chromosome No. 22 of a tobacco genome, and the nucleotide type is A or G. The application further discloses a KASP primer group for detecting the SNP marker and application thereof. The SNP marker of the application is a long-flower tobacco resistance site, and 80%-90% of non-target materials can be eliminated through seedling stage molecular screening in the breeding process, so that the labor and material costs of field management and phenotype identification are significantly reduced, and the SNP marker has a wide application prospect.