39 results about "Gene module" patented technology

The invention discloses a key gene identification method related to tobacco nitrogen response, which comprises the following steps: S1, setting four nitrogen fertilizer gradient treatments on the basis of same phosphorus and potassium fertilization by adopting a field experiment of a completely random block; s2, randomly taking the 6th to 8th leaves of the five plants in each area, and dividing a sample into two parts: quickly freezing one part with liquid nitrogen, and storing at-80 DEG C for RNA (Ribonucleic Acid) extraction and transcriptome sequencing; one part is used for measuring the nitrogen content, and after baking and drying treatment, a KjeltecTM8100 automatic nitrogen analyzer is used for measuring; s3, nitrogen content determination: determining the nitrogen content in the treated sample by using an automatic nitrogen analyzer KjeltecTM8100; according to the method, high-throughput transcriptome sequencing and weighted gene co-expression network analysis (WGCNA) are combined, so that not only can gene expression maps of flue-cured tobacco leaves treated by different nitrogen fertilizers be comprehensively captured, but also gene modules with similar expression modes can be mined from a global perspective.

The invention provides a gene module analysis method and device, equipment and a storage medium, and relates to the technical field of space transcriptomics. The method comprises the steps of obtaining space-time group chip expression data, performing linearization processing, and performing sliding window cutting to obtain a plurality of window units arranged according to a space sequence; calculating gene function activeness and constructing a matrix; clustering and grouping are carried out to obtain window groups; performing difference analysis screening on the window groups to obtain a difference expression gene set; and performing gene module analysis to obtain a functional gene module. According to the method, through an integrated processing flow of structure linearization, sliding window cutting, activeness quantification, clustering analysis and module identification, fine extraction and function reconstruction of spatial expression information in the curled tissue are realized under the condition that an additional algorithm or external data is not introduced; and the practicability and research value of the spatial omics data in a complex organization structure are improved.

The invention belongs to the technical field of biology, and particularly relates to application of a GmNIP5-GmPKM20 gene module in regulation and control of nodulation of leguminous plants, the GmNIP5-GmPKM20 gene module is one or more of GmNIP5 and genes interacting with the GmNIP5, by analyzing the influence of the transgenic GmNIP5 and the genes GmPKM20 interacting with the GmNIP5 on soybean nodulation, it is clear that the GmNIP5 promotes infection of nodule bacteria and formation of root nodules, and the GmNIP5-GmPKM20 gene module can be used for regulating and controlling nodulation of leguminous plants. GmPKM20 inhibits symbiotic nodulation signals of soybeans, and it is verified that GmPKM20 and GmPKM20 can directly interact with each other.

The invention belongs to the field of disease evolution trajectory prediction in precision medicine, and discloses a Parkinson's disease evolution prediction modeling method based on high-order gene communication tensor. According to the method, firstly, based on an intercellular communication mechanism, a high-order gene module communication tensor is constructed as spatial topological data, so that an information interaction mode between gene modules is described; secondly, a self-adaptive Transform module is designed to extract gene expression features and high-order space topology features at the same time; and finally, integrating multi-view interaction information between the high-order gene module communication tensor and the whole blood transcriptome data through a multi-view collaborative fusion mechanism to realize effective fusion and utilization of features. Experimental results show that compared with a traditional genetic map, the high-order space representation provided by the invention has finer resolution capability, a plurality of evaluation indexes are remarkably improved, and a new technical means is provided for accurate staging of Parkinson's disease.

The invention relates to the technical field of biology, in particular to a gene co-expression network-based sepsis marker screening method and application. The screening method comprises the following steps: screening differential expression genes; performing hierarchical clustering analysis on each differential expression gene to combine each differential expression gene into a plurality of target gene modules; and screening out hub genes from each target gene module. According to the method, gene expression data and clinical feature data are combined, the accuracy of screening sepsis marker genes is improved, possible abnormal genes irrelevant to the pathophysiological process of sepsis are avoided, gene modules are divided more finely through primary clustering analysis and secondary clustering analysis, GO function analysis is combined, and the accuracy of screening the sepsis marker genes is improved. According to an analysis result, selection of a GO term subset is optimized, and a step length set by primary clustering is corrected, so that the fine granularity and accuracy of analysis are improved, division of gene modules is optimized, and more refined treatment and screening of markers of sepsis are realized.

The invention belongs to the technical field of molecular breeding, and particularly relates to application of an E1-SOC1 gene model in cultivation of wide-adaptability soybean varieties. Research finds that three genes (E1 and homologous genes E1La and E1Lb) of an E1 family and two genes (SOC1a and SOC1b) of an SOC1 family are knocked out from soybeans at the same time by utilizing a gene editing technology, and the obtained homozygous five mutants show wide adaptability when planted under field conditions of different latitude regions; and the method has certain wide adaptability in the aspects of plant height, section number, flowering period, mature period and yield. It is indicated that the E1-SOC1 gene module (E1, E1La, E1Lb, SOC1a and SOC1b genes) is expected to be applied to molecular design breeding for breeding wide-adaptability soybean models, and has important application prospects in soybean molecular breeding research.

The invention discloses an identification method, device and system for a high gene module scoring area, and relates to the technical field of space transcriptomics. The identification method comprises the following steps: acquiring space transcriptome data, dividing cells into a plurality of unit grids according to space coordinate information of the cells, and determining corresponding grid space coordinates for each unit grid; calculating a module score value of each cell and determining a high-score cell set; calculating an integrated gene set scoring index; and carrying out region division on the unit grid and determining a high gene module scoring region. According to the method, a plurality of genes are analyzed as a functional whole, the limitation that traditional single-gene analysis cannot reveal collaborative regulation is overcome, spatial quantization of a gene set synergistic effect is realized, and the problem that a gene set enrichment region cannot be positioned in the prior art is solved.

The invention belongs to the technical field of cell data processing, and relates to a single cell gene characterization system and method based on deep learning. The system comprises a gene information input module, a data processing module, a context sensing interpolation module, a gene feature fusion module, a deep representation learning and joint optimization module and a cell typing and differentiation track inference module. The input module integrates a data source; the processing module preprocesses and enhances the original expression matrix; the interpolation module performs weighted interpolation by using a conditional Gaussian graph model; the gene feature fusion module generates a gene module activity matrix through 1 * 1 convolution and the like; the fusion module fuses the sample meta-information with the active matrix, the sequencing depth and the gene feature vector; the depth representation module captures high-dimensional dependence through a multi-layer Transform network, and generates cell high-order potential representation; the typing module outputs a cell population division and differentiation trajectory based on the deduced pseudo-time trajectory. The method has the beneficial effects that cell expression characteristics are recovered under high sparseness and noise, and a new way is provided for cell type identification and the like.

Provided herein are stoichiometric Gas Vesicle Expression Systems (GVES), sets of polynucleotide constructs, and related vectors, cells, compositions, and methods configured for robust expression of Gas Vesicle Gene Clusters (GVGCs) in mammalian cells, particularly primary and immune cells. The GVES comprises distinct gene modules for the primary structural protein (gvpA / B) and assembly factors (AF1, AF2), operably configured to achieve a stoichiometric expression ratio, quantified by Dosage Index (DI), wherein the gvpA / B module is expressed at least 2-fold higher than the AF1 and AF2 modules.

The application discloses a method for constructing a gene regulation network based on meta-analysis, and aims to solve the problem that a gene regulation network has a large error and a low accuracy because different research data are not completely homogeneous when the gene regulation network is expanded by combining data of multiple studies to enlarge a sample size, and the method comprises the following steps: performing meta-analysis on each transcriptomic gene expression dataset obtained to obtain a significant differential gene list; obtaining a transcription factor gene list according to a species to which the transcriptomic gene expression data belong, and generating a core gene list according to the transcription factor gene list and the significant differential gene; calculating a Pearson correlation coefficient of each core gene and each significant differential gene, and constructing a co-expression network according to the Pearson correlation coefficient; clustering the co-expression network to obtain a plurality of differential gene modules with high correlation of the core genes; and combining biological knowledge and a structural equation model to construct a corresponding gene regulation network according to each module. The application belongs to the field of gene regulation networks.

Provided herein are stoichiometric Gas Vesicle Expression Systems (GVES), sets of polynucleotide constructs, and related vectors, cells, compositions, and methods configured for robust expression of Gas Vesicle Gene Clusters (GVGCs) in mammalian cells, particularly primary and immune cells. The GVES comprises distinct gene modules for the primary structural protein (gvpA / B) and assembly factors (AF1, AF2), operably configured to achieve a stoichiometric expression ratio, quantified by Dosage Index (DI), wherein the gvpA / B module is expressed at least 2-fold higher than the AF1 and AF2 modules.

The application discloses application of a 5D006949-1D001661-3C003541 gene module in regulating oat crude protein content and belongs to the technical field of plant molecular biology. The nucleotide sequence of 5D006949 is shown in SEQ ID NO. 1, and the amino acid sequence is shown in SEQ ID NO. 2; the nucleotide sequence of 1D001661 is shown in SEQ ID NO. 3, and the amino acid sequence is shown in SEQ ID NO. 4; and the nucleotide sequence of 3C003541 is shown in SEQ ID NO. 5, and the amino acid sequence is shown in SEQ ID NO. 6. By analyzing the coding sequences of the genes in the module, VIGS strains and overexpression transgenic oat strains are constructed to evaluate the functions of the genes in the gene module in regulating the crude protein content of oats. The results show that the gene module can regulate the crude protein content of oats, thereby providing a reference basis for genetically improving cultivated oats by using genetic engineering technology and providing a new target for cultivating new oat varieties with high yield and high quality.

The invention relates to the technical field of gene engineering, in particular to a bidirectional promoter Para *, an araa * expression system, application and an engineering strain. The araa * expression system comprises the bidirectional promoter Para *, a repressor protein araR gene module is arranged on one side of the bidirectional promoter Para *, and a fluorescent protein gene module is arranged on the other side of the bidirectional promoter Para *. Wherein the repressor protein araR gene module comprises a repressor protein araR gene and a first RBS sequence corresponding to the repressor protein araR gene; and the fluorescent protein gene module comprises a fluorescent protein gene and a second RBS sequence corresponding to the fluorescent protein gene. By characterizing and designing an exogenous inducible promoter, an ara * expression system capable of realizing inducible expression in xanthomonas campestris is obtained, and a basic element is provided for construction of a xanthomonas campestris genetic engineering strain.

The invention discloses a Chinese rose HSF gene and application thereof, the Chinese rose HSF gene comprises an RcHSFA2 gene or an RcHSFC1 gene, and the nucleotide sequences of the RcHSFA2 gene or the RcHSFC1 gene are respectively shown as SEQ ID No.1 and SEQ ID No.3; the amino acid sequences are respectively as shown in SEQ ID No.2 and SEQ ID No.4; the two genes are subjected to high-temperature stress induced expression, and the protein RcHSFA2 and the protein RcHSFC1 interact with each other; the two genes are respectively and independently overexpressed in tobacco, so that the heat resistance of the transgenic tobacco is remarkably improved; the two genes are respectively and independently overexpressed in the Chinese rose petals, so that the heat resistance of the transgenic Chinese rose petals is also remarkably improved; according to the present invention, the RcHSFA2-RcHSFC1 gene module is co-expressed in the Chinese rose petal so as to significantly improve the heat resistance of the Chinese rose compared to the single overexpression of RcHSFA2 or RcHSFC1;

The invention relates to the technical field of plant genetic breeding, in particular to a directional pyramiding breeding method for improving the pungency degree and color value of processed chilies. Comprising the steps of parent screening (molecular marker identification of high-pungency and high-color-value germplasm based on capsaicin synthesis key genes Pun1 and Capana06g001204 and capsorubin regulatory genes CaBBX20 and CCS), hybridization polymerization (multi-generation backcross and molecular marker-assisted selection integration of pungency degree / color value genes), ploidy breeding accelerated homozygosis (a microspore culture combined chromosome doubling technology), and cross breeding of the high-pungency and high-color-value germplasm. And performing phenotype verification (detecting the content of capsaicin substances by high performance liquid chromatography and determining the color value by spectrophotometry). According to the method, gene modules for controlling the pungency degree and the color value are directionally polymerized, a special dry processing variety with the capsaicin content being larger than or equal to 3% (the corresponding pungency degree being larger than or equal to 30,000 SHU) and the color value being larger than or equal to 300 ASTA is bred, the breeding period is shortened to 1 / 3 of that of a traditional method, and the requirement of the pepper processing industry for high-quality raw materials is met. The method is suitable for large-scale pepper breeding, and is particularly suitable for planting in high-altitude and arid regions.

The invention provides a preparation method and application of a microorganism for producing 1-phenethyl isoquinoline. Specifically, the invention provides an engineering microorganism for producing 1-phenethyl isoquinoline, and the engineering microorganism contains exogenous and / or endogenous gene modules, such as gene modules of TyrH, PCD, DHPR, DODC, 4CL, TAL, CCR, AER, NCS and the like. The invention also provides a carrier, a kit and a fermentation system for producing the 1-phenethyl isoquinoline, a method for constructing the engineering microorganism and a method for producing the 1-phenethyl isoquinoline. According to the invention, a heterologous biosynthesis approach is successfully established, efficient de novo synthesis of the 1-phenethyl isoquinoline is realized by utilizing a simple carbon source, the process flow is simplified, the yield of the 1-phenethyl isoquinoline is increased, and a foundation is laid for large-scale preparation of PIA natural products.

The application relates to the field of bioinformatics and clinical auxiliary decision-making technology, in particular to a high-dimensional weighted membranous nephropathy gene co-expression network construction method, which integrates biomolecular interaction, disease pathways and health baseline data to construct an initial topological matrix, uses a Riemann manifold optimization algorithm to inject high-dimensional transcription sequencing data into the matrix and update edge weights, generates a high-dimensional weighted network of a target biological sample, accurately locks an abnormal gene module by calculating the topological residual error of the network and the health baseline and the dynamic offset of the eigenvector centrality, compares module features with a target point database to screen candidate drug targets, and stores network weight eigenvalues into a vector database, so that gene co-expression network construction and drug target screening based on biological priori and data driving are realized; and the application accurately retains weak but key local nonlinear cascade signals.

The invention relates to a single cell data enhanced representation method and device and a storage medium. The method comprises the following steps: acquiring an original counting matrix and a gene module data set of single cell gene expression; performing gene feature conversion based on the original counting matrix to obtain a gene feature matrix; determining a cell module activity matrix based on the gene feature matrix, the original counting matrix and the gene module data set; processing the gene feature matrix through a gene encoder to generate a gene implicit vector, and processing the cell module activity matrix through a module encoder to generate a module implicit vector; performing interactive fusion by taking the gene implicit vector and the module implicit vector as input of a cross attention fusion model and taking the weighted sum of the first loss item and the second loss item as an optimization target to obtain an output fusion implicit vector; and projecting the fusion implicit vector to a target embedding space to generate single cell enhanced representation. Implicit vectors are integrated through a cross attention fusion model, and the interpretability of unicellular organisms is improved.

The invention provides an H3K27M mutant type diffuse midline glioma accurate drug screening method and a prognosis model, and the method comprises the following steps: (1) analyzing differential genes of H3K27M mutant type and wild type diffuse midline glioma, identifying gene modules related to H3K27M mutation, analyzing intersections of the differential genes and the gene modules, and screening the intersections of the differential genes and the gene modules; obtaining a robust differential gene and analyzing an enrichment pathway of the robust differential gene; (2) screening drugs in the targeted H3K27M key differential pathway: screening the drugs in the drugs capable of interfering with the enriched pathway obtained in the step (1); (3) screening drugs for targeted H3K27M robust differential genes: analyzing prognosis-related genes in the robust differential genes obtained in the step (1), and carrying out drug screening in drugs capable of intervening in the prognosis-related robust differential genes; and (4) screening drugs for targeted H3K27M key characteristic genes: analyzing the genes related to poor prognosis, and screening the drugs in the drugs influencing the expression of the genes related to poor prognosis. According to the invention, a new thought is provided for screening the diffuse midline glioma drugs.

The invention provides a circadian rhythm evaluation method and system oriented to cardiovascular states, and belongs to the field of transcriptional gene analysis, and the method comprises the steps: obtaining transcriptome data at a single time point; gene expression data are positioned and extracted from the transcriptome data through a preset rhythm homeostasis gene module, and the rhythm homeostasis gene module is constructed through a weighted gene co-expression network analysis algorithm; and calculating the collaboration, the volatility and the network concentration degree of the gene expression data based on a dynamic network biomarker algorithm, and determining the circadian rhythm disorder index of the to-be-evaluated user according to the collaboration, the volatility and the network concentration degree. According to the method, collaborative expression characteristics are systematically captured by using a rhythm homeostasis gene module, dependence on a small number of core clock gene signals is avoided, and quantitative calculation is carried out on the collaboration, volatility and network concentration ratio of gene expression data in the module based on a dynamic network biomarker algorithm; and an objective and quantifiable molecular evaluation index is provided for an individual rhythm state.

The invention provides an engineering bacterium for producing vitamin B6 as well as a construction method and application of the engineering bacterium. Specifically, the engineering bacterium contains an exogenous 5 '-phosphopyridoxal synthetase expression cassette, the expression cassette contains a PdxST gene module or a PdxTS gene module, and the PdxST gene module and the PdxTS gene module respectively and independently contain the following genes: a PdxS gene and a PdxT gene; wherein the PdxS gene and the PdxT gene are respectively and independently derived from microorganisms selected from the following group: streptococcus pneumoniae, staphylococcus aureus, bifidobacterium animalis, haematococcus acidilactici, nocoryococcus martensii or a combination thereof. The engineering bacterium greatly improves the production efficiency of the vitamin B6, is suitable for large-scale industrial production, and has great application and popularization values.

Provided herein are methods of treating cancer, inflammatory diseases, and autoimmune diseases, and methods of modulating related phenotypes and expression levels, by targeting interactions between E3 ligases, E3-like proteins, and interacting partners thereof. Methods of identifying modulators of such interactions are also provided. Also provided herein are altered cell therapies comprising at least two members of a co-functional gene module.

The invention discloses an application of an overexpressed BnaAHL19 gene in regulating and controlling the flowering time of brassica napus. A DNA (deoxyribonucleic acid) nucleotide sequence of the BnaAHL19 gene is shown as SEQ ID NO.1, and a coded amino acid sequence is shown as SEQ ID NO.2. The invention further comprises a module containing the BnaAHL19 gene to the BnaELF-BnaAHL19-BnaFLC gene, and application of the BnaELF-BnaAHL19-BnaFLC gene module to regulation and control of flowering time of the brassica napus, the BnaAHL19 and the interactive protein BnaELF thereof can cooperatively activate expression of a downstream gene BnaFLC, and the BnaAHL19 and the interactive protein BnaELF thereof can be used for regulating and controlling flowering time of the brassica napus. The function excavation of the BnaELF-BnaAHL19-BnaFLC module has important significance on promoting the genetic improvement and breeding of the brassica napus in the growth period.

The application discloses a tylosin A high-yield engineering strain and application thereof, and belongs to the field of genetic engineering and fermentation technology. The strain is obtained by integrating a carbon mycose synthesis gene module (M14) overexpression cassette driven by a constitutive strong promoter into a genome and knocking out a negative regulation gene tylQ , so that the yield is greatly improved. Experiments show that the yield of the engineering strain in FFM culture medium reaches 1183 mg / L, which is 10 times that of a wild type; the yield can reach 1491.5 mg / L after adding alpha-ketoglutaric acid, and the genetic stability is good. The 5L fed-batch fermentation titer reaches 5.63 g / L. The application provides an efficient and stable industrial strain for large-scale production of tylosin A.

The present application relates to the field of disease prediction in bioinformatics, and particularly relates to a cognitive disease classification method based on gene communication mode and multi-view learning. The present application uses a gene network reasoning algorithm to operate on the screened sample-gene expression matrix to obtain a gene regulatory network; combines the importance score of the gene regulatory network and the gene module information to obtain a gene module communication tensor; respectively extracts features from the gene module communication tensor and the sample-gene expression matrix, and puts the extracted features into a Transformer encoder and an interleaved decoder for feature fusion to obtain odd layer and even layer features, splices the features of the two layers and puts them into a multi-layer perceptron to finally classify Alzheimer's disease into different stages. The present application provides a neural network framework for double-view learning of gene communication analysis of Alzheimer's disease, fuses two-view information of gene module communication tensor and transcriptome, and improves the accuracy of brain disease stage classification.

The invention relates to a gene module construction method and device, computer equipment and a storage medium. The method comprises the steps that gene sequencing is conducted according to pathological sample queue data, a transcription data set is obtained, the transcription data set comprises single cell data and space-time transcriptome data, transcriptome preprocessing is conducted on the space-time transcriptome data, and a transcriptome gene expression matrix is obtained; carrying out deconvolution processing according to the type of the single cell data and the transcriptome gene expression matrix to obtain embedded matrix data, carrying out gene module construction according to the embedded matrix data and a preset target gene construction model to obtain a gene module of each sample slice, carrying out similarity clustering on the gene modules to obtain a gene weight list, and storing the gene weight list in a database; and integrating the gene modules according to the gene weight list to obtain a gene hypermodule. Therefore, by adopting the method, the compatibility of the multi-modal data can be improved, the batch effect can be reduced, and the gene module construction accuracy can be improved.

The invention discloses a cancer cell mitochondrial biomarker and a screening method based on a machine algorithm, and when the cancer cell is colorectal cancer, the mitochondrial biomarker comprises OXCT1, CLPB, SLC25A12, MRPL51, SFXN1, GATM and TRMT10C genes. The screening method comprises the following steps: (1) standardizing an expression data set of cancer cells to be screened, and then obtaining the expression quantity of mitochondrial related genes in the expression data of the cancer cells; (2) difference analysis: carrying out correlation analysis on cancer cell mitochondrial genes or proteins subjected to differential expression, and selecting gene modules which simultaneously meet differential genes and belong to mitochondrial related genes; (3) functional enrichment analysis; and (4) respectively screening by selecting at least two machine algorithms to respectively obtain a characteristic gene or protein list, and determining a core intersection target. The biomarker disclosed by the invention is high in accuracy, specificity and sensitivity. The method is efficient and comprehensive.

The invention discloses an application of a 5D006949-1D001661-3C003541 gene module in regulating and controlling the content of crude protein of oat, and belongs to the technical field of plant molecular biology. Wherein the nucleotide sequence of the 5D006949 is as shown in SEQ ID NO. 1, and the amino acid sequence of the 5D006949 is as shown in SEQ ID NO. 2; the nucleotide sequence of the 1D001661 is as shown in SEQ ID NO.3, and the amino acid sequence of the 1D001661 is as shown in SEQ ID NO.4; the nucleotide sequence of the 3C003541 is as shown in SEQ ID NO.5, and the amino acid sequence of the 3C003541 is as shown in SEQ ID NO.6. By analyzing the coding sequence of the gene in the module, a VIGS strain and an overexpression transgenic oat strain are constructed to evaluate the function of the gene in the gene module for regulating and controlling the crude protein content of the oat, and the result shows that the gene module can regulate and control the crude protein content of the oat, so that the content of the crude protein in the oat can be regulated and controlled. A reference basis is provided for genetic improvement of cultivated oats by using a genetic engineering technology, and a new target is provided for cultivation of a new variety of high-yield and high-quality oats.

The invention provides an intelligent forest tree breeding method based on multi-modal data and a large model, and relates to the technical field of forest tree genetic breeding. The method comprises the steps of obtaining and integrating genotype data, phenotype data, multi-omics data and environment time sequence data of a target tree species, and performing preprocessing and feature fusion to form a multi-modal data set for analysis; on the basis of the multi-modal data set, key genes or gene modules for regulating and controlling target characters are mined by adopting a genotype-environment interaction algorithm and multi-omics coupling analysis; constructing a dynamic model fusing genotypes, environmental factors and time dimensions, and predicting breeding values of individuals or combinations on a whole genome level based on mined key genes or gene module information; and based on a prediction result, performing multi-objective optimization and intelligent screening at three levels of parents, combinations and offspring, and outputting a breeding decision scheme. According to the method, a complete, efficient and accurate forest tree intelligent breeding solution is formed.

This invention belongs to the fields of bioinformatics and computational biology, and specifically relates to a method, system, and storage medium for gene functional module mining based on tumor subtype supervision. The method includes the following steps: preprocessing breast cancer data and screening a unified gene set; calculating the difference in gene correlation coefficients before and after adding tumor samples in normal samples to construct a gene differential expression network; constructing a subtype-level gene graph structure through gene correlations, inputting it into a graph neural network to obtain an updated gene embedding representation; performing end-to-end training based on the gene embedding representation using differentiable minimum cut loss and subtype-supervised joint loss; and fusing the clustering results of samples within a subtype through majority voting to identify subtype-specific functional gene modules. This invention achieves the joint mining of cancer shared gene modules and subtype-specific gene modules, effectively improving the accuracy and biological interpretability of functional module identification.