Method for establishing human brain gene expression space-time norm

A technology of gene expression and method establishment, which is applied in the field of establishment of spatiotemporal norms of gene expression in the human brain, and can solve problems such as difficult comprehensive utilization and scattered database data resources

Active Publication Date: 2019-10-18
INST OF PSYCHOLOGY CHINESE ACADEMY OF SCI
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Problems solved by technology

[0004] In order to solve the existing large amount of high-quality and different types of human brain gene expression data, the present invention optimizes the problem that existing database data resources are scattered and difficult to comprehensively utilize by integrating the normal human brain gene expression data of different develop

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  • Method for establishing human brain gene expression space-time norm
  • Method for establishing human brain gene expression space-time norm
  • Method for establishing human brain gene expression space-time norm

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Embodiment Construction

[0046] The technical solutions of the present invention will be clearly and completely described below in conjunction with the accompanying drawings. Apparently, the described embodiments are some, but not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0047] Such as figure 1 As shown, the present invention provides a method for establishing a spatiotemporal norm of human brain gene expression, comprising the steps of:

[0048] 【S1】Collect the raw data of the brain, analyze and calculate, and obtain the standardized expression data set of all genes in each sample;

[0049] There are three sources of brain raw data collected in the present invention. One is the need to analyze the original data: first download the original data from the NCBI high-throughput next-genera...

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Abstract

The invention discloses a method for establishing a human brain gene expression space-time norm. The method comprises the following steps: collecting brain space-time original data to obtain a gene standardized expression data set; constructing a two-dimensional space-time framework of a brain region and a development stage, and enabling all gene standardized expression data to correspond to the constructed space-time framework according to the brain region and development stage information to obtain a single-gene space-time expression mode; analyzing the obtained single-gene space-time expression matrix data by adopting a gene co-expression network analysis method to obtain a plurality of gene modules, and averaging the gene expression quantities of the gene modules to obtain a gene module space-time expression mode; analyzing the normal human nerve cell expression data, and performing the enrichment analysis of characteristic genes of nerve cells and the obtained gene modules to obtain a cell expression mode of the gene module. According to the method, all reference data sets are finally presented in the same two-dimensional space-time framework, so that the data sets from different sources are integrated, and researchers can intuitively supplement and compare the content of the space-time framework.

Description

technical field [0001] The invention relates to the technical field of human brain modeling, in particular to a method for establishing a spatiotemporal norm of human brain gene expression. Background technique [0002] The development of the human brain occurs over a long period of time, with extremely complex and precise regulation. From embryonic stage to adulthood and various stages of aging, the human brain undergoes long-term development, refinement and maturation, undergoing molecular, connection and structural changes. Provide insights into susceptibility to certain brain disorders such as neurodegenerative and psychiatric disorders by studying gene expression patterns in regions of normal brain function, developmental stages, and different neural cell types. [0003] A large amount of high-quality gene expression data of different types in the human brain is available. Considering the difference in gene length and sequencing depth in RNA-seq data analysis, it is a...

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Application Information

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IPC IPC(8): G16B25/00G16B5/00G16B40/00G16B50/30
CPCG16B25/00G16B5/00G16B40/00G16B50/30
Inventor 王晶郭黎媛林葳
Owner INST OF PSYCHOLOGY CHINESE ACADEMY OF SCI
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