9 results about "Transcription regulator" patented technology

The present invention relates to a gene variant of the transcriptional regulator LysG and a method for producing L-citrulline or L-arginine using same. The gene variant of the transcriptional regulator LysG according to the present invention has protein activity that is altered due to the mutation of one or more bases in the base sequence of a gene encoding the transcriptional regulator LysG, and thus, it is possible to effectively produce L-citrulline or L-arginine from a recombinant microorganism comprising the variant.

Genetically modified cells that are compatible with multiple subjects, e.g., universal donor cells, and methods of generating said genetic modified cells are provided herein. The universal donor cells comprise at least one genetic modification within or near a gene that encodes one or more MHC-I or MHC-II human leukocyte antigens or a component or a transcriptional regulator of a MHC-I or MHC-II complex, wherein genetic modification comprises an insertion of a polynucleotide encoding a tolerogenic factor and / or survival factor. The universal donor cells may further comprise at least one genetic modification within or near a gene that encodes a survival factor, wherein said genetic modification comprises an insertion of a polynucleotide encoding a second tolerogenic factor and / or a different survival factor.

The present invention relates to transcriptional regulator polypeptides, polynucleotides encoding the transcriptional regulator polypeptides, and to nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing a polypeptide of interest in host cells overexpressing the transcriptional regulators, to methods of increasing the oxygen uptake rate and / or decreasing viscosity of the culture broth in a fungal host cell cultivation process, to methods of producing a transcriptional regulator polypeptide, to the use of a transcriptional regulator polypeptide, and to the production of fungal biomass.

The present disclosure generally relates to multi-chain chimeric polypeptides or receptors having distinct polypeptide chains that associate post-translationally to enable the simultaneous activation of the signaling domain and the release of a transcriptional regulator upon binding of a ligand. The disclosure also provides nucleic acid constructs, recombinant cells, vectors, pharmaceutical compositions, and methods of treatment including the multi-chain chimeric polypeptides of the disclosure. The disclosure further provides methods for simultaneously inducing T cell signaling and gene regulation in a T cell.

Genetically modified cells that are compatible with multiple subjects, e.g., universal donor cells, and methods of generating said genetic modified cells are provided herein. The universal donor cells comprise at least one genetic modification within or near at least one gene that encodes one or more MHC-I or MHC-II human leukocyte antigens or component or transcriptional regulator of the MHC-I or MHC-II complex, at least one genetic modification that increases the expression of at least one polynucleotide that encodes a tolerogenic factor, and optionally at least one genetic modification that increases or decreases the expression of at least one gene that encodes a survival factor.

This invention provides a combined module and its application for enhancing the symbiotic nitrogen fixation ability of rhizobia, belonging to the field of synthetic biology. The combined module for enhancing the symbiotic nitrogen fixation ability of rhizobia includes sequentially connected inducible promoter elements Pi that specifically respond to symbiotic nitrogen fixation signals and elements from... Sinorhizobium Fred transcriptional regulatory factor encoding genes tetR The present invention inserts the combined module into the initial vector to construct a recombinant expression vector, and then uses the triparental conjugation method to transfer the recombinant expression vector into different soybean rhizobium chassis to obtain two nitrogen-fixing recombinant engineered strains. Compared with wild-type rhizobium, the recombinant engineered strains of the present invention can restore the effect of glyphosate stress on the symbiotic nitrogen-fixing ability of rhizobium.

The present application relates to a microorganism having weakened activity of a LacI DNA-binding transcriptional regulator protein and a method for producing L-glutamic acid using the same. The Corynebacterium microorganism of the present application having weakened activity of a LacI DNA-binding transcriptional regulator protein has significantly increased L-glutamic acid producing ability, and its use makes it possible to produce L-glutamic acid at a higher yield than existing microorganisms.

The application discloses an Lrp mutant and application thereof. The application relates to an Lrp mutant and application thereof in the field of biotechnology. The leucine-responsive transcriptional regulator mutant provided by the application is any one of the following: A1) a protein obtained by mutating a histidine at the 107th position of a protein with an amino acid sequence of SEQ ID No: 1 into tyrosine; A2) a protein obtained by substituting, deleting and / or adding amino acid residues of the protein of A1) and having 75% or more identity with the protein shown in A1) and the same function; and A3) a fusion protein obtained by connecting a protein tag to the N terminal or / and C terminal of A1) or A2). The yield of L-valine of the engineering strain is improved through site-directed mutation, and the application has industrial application value.

Provided is a method for increasing the productivity of secondary metabolites such as antibiotics in a microorganism. In particular, provided is a method to improve the productivity of secondary metabolites in a microorganism by inactivating or reducing the expression of FadR transcription regulator and Srb RNA binding protein of said microorganism.