The invention discloses a
gene sequence with a
molecular switch, a
plasmid and an immune
cell, and belongs to the technical field of
molecular biology. The
gene sequence comprises an excitation component, a connection region and a response component, wherein the excitation component comprises a
target binding region and a transcription regulation region; the response component comprises an expression region and a
promoter region matched with the transcription regulation region; the
target binding region of the excitation component is used for expressing a
target binding receptor; the transcription regulation region is used for expressing a transcription regulation factor; after the target binding
receptor is used for being bound with a target, the transcription regulation factor is separated from the target binding
receptor, is bound to the
promoter region, and activates a
promoter to start expression of the
gene sequence in the expression region; and the connection region connects the excitation component and the response component in the same
gene sequence. The preparation flow is simplified, the
transfection efficiency is improved, and industrial preparation of
virus particles and CAR cells is facilitated.