21 results about "Transcriptional Regulatory Elements" patented technology

This application relates to the field of molecular biology, and particularly to a customized and universal amino acid sensor and its application. This application discloses an amino acid sensor, which includes a constitutive promoter, the coding gene of the natural transcriptional regulatory element amino acid attenuator of Escherichia coli, a reporter gene, and a terminator connected in sequence. Through element transformation, signal mechanism innovation, and reaction system optimization, this application has constructed a new generation of amino acid detection technology system, achieving breakthrough improvements in core indicators such as detection sensitivity, specificity, and application scenarios, providing an innovative technical solution for the field of biosensing.

Nucleic acid molecules comprising at least one transcription regulatory element operably linked to an open reading frame, wherein the open reading frame encodes a single RNA transcript encoding GCaMP7f, a ribosomal skipping peptide, and a fusion protein of a nuclear protein and photoactivatable red fluorescent protein are provided. Expression vectors and cells comprising the nucleic acid molecules are also provided, as are methods of using the nucleic acid molecules for simultaneous labeling and measuring calcium and analyzing a target cell.

The invention discloses a regulatory element for enhancing the activity of a promoter and a method for obtaining a high-activity promoter through transformation, and relates to the technical field of gene engineering. The technical problem to be solved by the invention is how to obtain a modified promoter with improved promoter activity. In order to solve the technical problem, the invention provides transcriptional regulation elements from the 1st site to the 1200th site of a nucleotide sequence such as SEQ ID NO: 1. By connecting the transcriptional regulation element to the upstream of a 35S core promoter, a high-activity promoter with the promoter activity 5-6 times that of the 35S promoter can be obtained. According to the research technical scheme, a new insight is provided for analyzing the functional architecture of the corn cis-regulatory element, and a theoretical basis is laid for the rational design of crop synthesis promoters.

The disclosure features Rep / Helper plasmids comprising a cytomegalovirus (CMV) promoter, an E2A gene, an E4 gene, a VA gene and a rep gene, or a cytomegalovirus (CMV) promoter, an E2A gene, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), an E4 gene, a VA gene and a rep gene, or an E2A gene, an E4 gene, a VA gene, a Kozak consensus sequence and a rep gene, where the plasmid does not comprise a nucleic acid sequence encoding a cap gene.

To provide a safe and effective mutated woodchuck post-transcriptional regulatory element (WPRE) for use in a retroviral vector.SOLUTION: Provided is a vector comprising a mutated woodchuck post-transcriptional regulatory element (WPRE), wherein the vector does not include an X protein promoter, and the mutated WPRE does not include an X protein open reading frame (ORF).SELECTED DRAWING: None

The present disclosure relates to transcriptional regulatory elements comprising an enhancer and a promoter, and polynucleotides, vectors and cells comprising the same. Also provided are uses of the transcriptional regulatory elements for inducing cardiac-specific gene expression, and methods of treating or preventing a cardiomyopathy comprising administering a vector comprising the transcriptional regulatory elements.

The present disclosure provides inducible constructs comprising transcription initiation and / or transcriptional regulatory elements. These constructs can be used to drive gene expression in response to specific cellular conditions (e.g., in the context of antigenic stimulation). In some embodiments, the constructs are incorporated as part of regulatory circuits useful for controlling the expression and regulation of cells expressing the engineered receptor.

The invention discloses a method for screening a transcriptional regulatory element and application thereof, relates to the technical field of genes, and provides a method for predicting a collaborative transcriptional regulatory element based on combination of single-cell multi-omics and experimental exploration. The potential synergistic effect between transcriptional regulation elements, chromatin remodeling complex interaction and other cross-level regulation events can be revealed, and a method is provided for recognition of disease key regulation nodes. On the other hand, the application systematically reveals key transcriptional regulatory elements involved in disease responses based on single cell multi-omics sequencing techniques. Through integration of single cell chromatin accessibility analysis and transcriptome sequencing data, interaction among a core transcription factor, an epigenetic regulation element and a signal transduction pathway for driving disease generation is clarified, and the mononuclear cell specific epigenetic-transcriptional regulation network is successfully constructed.

The present invention provides gene therapies for the treatment of Infantile Neuroaxonal Dystrophy. The invention provides a viral vector comprising a viral capsid and an expression cassette comprising a nucleic acid encoding the PLA2G6 gene. The expression cassette comprises, in order, a SYN1 promoter or an EF1a promoter, a nucleic acid sequence encoding a PLA2G6 gene, and a poly(A) signal. Advantageously, the expression cassette does not comprise a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) between the nucleic acid sequence encoding a PLA2G6 gene and the poly(A) signal.

The invention belongs to the technical field of gene therapy, and particularly relates to a recombinant expression system for expressing human GLB1 protein, a protein expression cassette, a recombinant adeno-associated virus vector and application of the recombinant adeno-associated virus vector in prevention and treatment of GM1 ganglioside storage diseases. In order to solve the problem that in an existing GLB1 gene expression system, due to the fact that a transcriptional regulation element combination is not systematically optimized, the expression efficiency in a central nervous system is insufficient, the codon-optimized human GLB1 gene and various different promoter-intron combinations are subjected to systematic pairing and functional verification; it is found that a recombinant expression system formed by a CB7 promoter-chimeric intron 1-codon optimized human GLB1 gene (SEQ ID NO: 2) is packaged into a recombinant adeno-associated virus AAV9.CB7. HGLB1co.bGH, the effect of treating the GM1 ganglioside storage disease is most remarkable, and the GM1 ganglioside storage disease is expected to be efficiently and safely prevented and radically treated.

To provide regulatory elements, as well as vectors containing the same that may be used to stimulate transcription of a gene of interest in certain tissue types.SOLUTION: Transcription regulatory elements may be operably linked to a transgene, such as acid α-glucosidase (GAA), so as to promote expression of the GAA transgene in a cell, such as a muscle cell, liver cell, or neuron. The transcription regulatory elements described herein may be operably linked to a therapeutic transgene and used for the treatment of various disorders, such as lysosomal storage diseases, and particularly Pompe disease.SELECTED DRAWING: Figure 1

The invention relates to heterozygous regulatory elements. Specifically, the present invention relates to hybrid transcriptional regulatory elements, in particular hybrid promoters, that drive gene expression, designed by fusion of at least two transcriptional regulatory elements having different tissue selectivity, for example two promoters that drive expression in different tissues in a tissue-selective manner.

The present disclosure relates to transcription regulatory elements for gene therapy, and recombinant adeno-associated viral vectors comprising the same. The present disclosure also relates to the use of the transcription regulatory elements and recombinant adeno-associated viral vectors in therapy.

The invention innovatively provides a novel cellulose response type posttranscriptional regulatory element based on RNA conformation change. The regulation element comprises a core hairpin structural domain and a downstream terminator structural domain which are connected in series; the nucleotide sequence of the core hairpin structural domain is as shown in SEQ ID NO: 1, and the nucleotide sequence of the downstream terminator structural domain is as shown in SEQ ID NO: 2. The cellulose response type post-transcriptional regulation element disclosed by the invention has high-temperature applicability, can ensure the stability and responsiveness of a hairpin structure and a terminator at high temperature at the optimal growth temperature (55-60 DEG C) of clostridium thermocellum, and solves the core problem of scarcity of inducible tools in high-temperature chassis bacteria. On the basis, the invention further provides an engineering strain, the problem that the thermophilic bacterium clostridium thermocellum lacks a substrate induction type expression tool is solved, precise coupling of gene expression and a cellulose substrate is achieved, and the saccharification efficiency of lignocellulose and the metabolic engineering application potential can be remarkably improved.

The invention belongs to the technical field of genetic engineering, particularly relates to creation and application of a ZmRap2.7 regulatory region edition-based corn early blossoming and yield conservation material, and more particularly relates to a method for regulating and controlling corn early blossoming and yield conservation, a biological material used by the method and application of the biological material. The technical problem to be solved by the invention is how to prepare the corn with early flowering and yield conservation. In order to solve the technical problem, the invention provides a method for preparing the corn with the early blossoming and yield keeping functions, the method comprises a step of performing gene editing on a transcription regulation element of a ZmRap2.7 gene in target corn to obtain the corn with the early blossoming and yield keeping functions, and the transcription regulation element comprises a Vgt1 enhancer or / and a ZmRap2.7 promoter. According to the application, the expression of the ZmRap2.7 gene is specifically regulated and controlled by precisely regulating and controlling the Vgt1 enhancer or the ZmRap2.7 promoter, and the goal of early flowering and yield conservation in agricultural production is achieved.

The present application relates to an engineered gene transcriptional repression tool targeting inhibin subunit βE (INHBE) and the use thereof, and specifically relates to a complex comprising a first fusion and a second fusion, wherein: 1) one of the first fusion and the second fusion comprises a DNA methylation domain and at least one recruitment domain A, and the other fusion comprises a transcriptional repressor domain and at least one recruitment domain A'; and 2) the first fusion or the second fusion comprises a nucleic acid-binding domain. Moreover, the recruitment domain A and the recruitment domain A' are capable of interacting with each other, such that one of the first fusion and the second fusion or a part thereof can be recruited to the vicinity of the other fusion; and the nucleic acid-binding domain is capable of specifically binding to an INHBE gene transcriptional regulatory element.

Provided herein are polynucleotides comprising at least two coding sequences for expression of coding sequences of interest in a cell and two transcriptional regulatory elements. The transcriptional regulatory elements may be tissue specific. Also provided are vectors, recombinant viral genomes, and recombinant virus compositions comprising said polynucleotides, as well as associated methods.

The application discloses a kind of microorganism manufacturing yield increasing methods based on gene dynamic down-regulation algorithm, it is related to the cross technical field of synthetic biology, metabolic engineering and computational biology, for rationally designing high-yield, robust microbial cell factory.The core of the method is to combine the static gene knockout target prediction, the data information collection of transcriptional regulatory element driven by large language model and the global metabolic correlation analysis based on graph theory, to realize the whole process rationalization design from "who to regulate" to "what to use", finally output the strain modification scheme that gene knockout (knockout) is converted into gene down-regulation (knockdown), to realize the efficient coupling of cell growth and product synthesis.

In the present invention, there is discovered an AAV vector that enables microglia-specific gene expression for a long period of time and can be used for the treatment of brain diseases. Specifically, it is found that a target gene can be expressed in a microglia-specific manner by using the following vector: an adeno-associated virus (AAV) vector for gene expression in a microglia, the vector including an expression unit that comprises an Iba1 (ionized calcium-binding adaptor molecule 1) promoter, a sequence complementary to miR-9, a sequence complementary to miR-129, and a Woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) gene, wherein the sequence complementary to miR-9 and the sequence complementary to miR-129 are located upstream of the WPRE gene, or the sequence complementary to miR-9 and the sequence complementary to miR-129 are located both upstream and downstream of the WPRE gene.

The invention discloses construction and application of an L-glutamine transcriptional regulation element, and belongs to the technical field of biosensors. The biosensor responding to the concentration of L-glutamine can be used for transcriptional regulation of a target gene, and belongs to the technical field of biochemical engineering. According to the invention, an L-glutamine binding protein and a regulatory protein BsGlnR are constructed on a pCDF series plasmid to obtain the L-glutamine transcriptional regulatory element. Through point mutation of a target DNA sequence and optimization of the structure of the regulatory protein BsGlnR, the inhibition capability of the transcription regulatory element is improved, and the transcription regulatory element has a very wide application prospect in the field of dynamic regulation of high-yield strain precursors.

The application discloses a bovine muscle development related enhancer Enh483 and application thereof in promoting bovine myoblast proliferation and / or differentiation. The application predicts and annotates the enhancer by IGV visual analysis of chromatin open regions by using existing buffalo myoblast proliferation period and differentiation period ATAC-Seq data and Chip-seq data, screens out a candidate enhancer Enh483 related to muscle development, and detects the enhancer activity by a dual luciferase vector report system. After the Enh483 activity is inhibited, the buffalo myoblast proliferation and differentiation are inhibited, which indicates that the Enh483 can promote the buffalo myoblast proliferation and / or differentiation. The application provides an important theoretical basis for the mechanism of the transcriptional regulatory elements such as enhancers in muscle development, and provides data support for buffalo breeding and strain selection.