9 results about "Minimal promoter" patented technology

The present disclosure provides high-performance immune-activation reporter cell line set. A set of nucleic acid constructs comprising one or more response elements operatively linked to a TATA box motif promoter or a minimal IL-2 promoter are provided. A set of reporter cell lines comprising a polynucleotide sequence encoding a reporter protein under the control of one or more response elements operatively linked to a TATA box motif promoter or a minimal IL-2 promoter is also provided. The provided set of reporter cells enable sensitive and signal-enhanced bioassay performance to reflect and evaluate adaptive immune cell activation / inhibition regulated by the immune receptors CD3, co-stimulatory CD28 and related signaling pathways, and avoid responding bias triggered by particular transcription factor. A controllable engineer method for a functional cell line is further provided.

The present invention provides viral vectors for use in the treatment and prevention of diseases associated with Schwann cells by delivering polynucleotides specifically to Schwann cells and achieving Schwann cell specific expression. The present invention has particular application in treatment and prevention of Charcot-Marie-Tooth disease and other demyelinating neuropathies. The preferred vectors are adeno-associated vectors (AAV) having a Schwann cell-specific promoter from the Myelin Protein Zero (Mpz, P0) or a minimal Mpz promoter.

The invention provides a generated superhigh-activity plant core promoter library. The superhigh-activity plant core promoter library comprises promoters with nucleotide sequences as shown in SEQ ID No.1-SEQ ID No.7; the invention also provides an application thereof. The method has the following advantages that the sequence characteristics of the plant core promoters are deeply excavated by utilizing an artificial intelligence technology, and the plant core promoters with ultra-high activity are designed and screened to form the promoter library. A corn protoplast system verifies that the activities of the seven plant core promoters in STARR-seq detection are all superior to those of a natural strong promoter UBI. In a dual luciferase (LUC) experiment, the activities of seven promoters all reach more than 16 times of that of a 35S minimum promoter, and the activities of two promoters are still higher than that of UBI. Therefore, the promoter library can effectively solve the problem that the activity of existing natural elements is limited, and the actual requirements of plant synthetic biology on ultrahigh-activity regulatory elements are met.

A construct comprising a promoter specific for non-pigmented ciliary epithelial cells (NPCECs) is provided. In particular, the construct comprises a BEST2 minimal promoter that confers NPCEC-specific expression. The BEST2 minimal promoter may be operably linked to an expressible sequence such as a gene encoding a polypeptide of interest, a regulatory RNA sequence, a reporter gene, and the like. Such constructs may be provided in an expression vector, for example, with the BEST2 minimal promoter operably linked to an expressible sequence or in a host cell genetically modified with such an expression vector.

The invention provides a recombinant biological carrier for detecting the activation efficacy of an aromatic hydrocarbon receptor and application, and belongs to the technical field of environmental protection. The invention provides a promoter for promoting expression of a reporter gene. The promoter comprises a plurality of core sequences of dioxin reaction elements and a minimum promoter. According to the invention, expression of MaxGFP is started by using the promoter, and an expression cassette and a recombinant biological carrier of the green fluorescent protein MaxGFP are constructed. The human cells are selected as the chassis cells, so that the subsequent detection application has species specificity, and the detection method is simple and convenient to operate, low in single detection cost and suitable for large-scale detection and human health risk assessment.

The invention discloses a cell sensor as well as a preparation method and application thereof. The present invention provides a cell sensor comprising a skin cell comprising a reporter gene operably linked to an exogenous chemical response element (XRE) and a minimum promoter (minP); wherein the reporter gene is expressed or activated when an AhR signal channel of the skin cell is activated, and a detectable signal is generated. The cell sensor provided by the invention can be used for rapidly screening the health risk of cosmetics, prompting the health risk caused by long-term exposure of various chemicals and commercial products, providing supplement for the existing method, and providing technical means and data support for perfecting related detection. The method is used for screening potential health risks of substances, such as cosmetics, in direct contact with skin, and can also rapidly indicate the toxicity of environmental pollutants and compounds.

The application provides a generated super-high-activity plant core promoter library, comprising promoters with the nucleotide sequences shown in SEQ ID No. 1-SEQ ID No. 7; and provides an application thereof. The application has the following advantages: the sequence characteristics of the plant core promoter are deeply mined by using artificial intelligence technology, and the super-high-activity plant core promoter is designed and screened to form a promoter library. The activity of the 7 plant core promoters in the application is better than that of the natural strong promoter UBI in the STARR-seq detection verified by the corn protoplast system. In the double luciferase (LUC) experiment, the activity of the 7 promoters reaches more than 16 times of that of the 35S minimum promoter, and the activity of 2 promoters is still higher than that of UBI. This shows that the promoter library can effectively solve the problem of limited activity of existing natural elements, and meet the actual needs of plant synthetic biology for super-high-activity regulatory elements.

The invention relates to the field of biological medicine, and discloses application of urine-derived stem cells in ED treatment. According to the scheme, a 9-copy hypoxia response element, a minimum promoter, a vascular endothelial growth factor and a nerve growth factor gene are introduced into urine-derived stem cells by utilizing a lentiviral vector, and a hypoxia response type gene modified cell system is constructed. Through local injection of the cavernous body, the cells accurately start factor expression in an anoxic microenvironment. According to the invention, deep coupling of vascular regeneration and neural restoration can be realized, expression can be automatically reduced along with rising of tissue oxygen content, excessive hyperplasia complications can be avoided, and closed-loop intelligent treatment of pathological induction-precise intervention can be realized.

Nucleic acid molecules configured to specifically express a sequence of interest in a cancer cell, comprising: a transcription factor binding region comprising 3-10 repeats of a transcription factor binding site, a minimal promoter and the sequence of interest operatively linked to the transcription factor binding region and minimal promoter, wherein the transcription factor is selected from SOX9, GATA10, TCF7L1, LEF1, MYBL2 and MYB are provided. Nucleic acid molecule systems and compositions comprising the nucleic acid molecules are also provided as are methods of treating cancer.