53 results about "Chlamydia" patented technology

The invention discloses a primer group and a kit for simultaneously detecting nucleic acids of various respiratory pathogens based on twenty-six PCR (Polymerase Chain Reaction). The kit comprises primer pool freeze-dried powder of influenza A virus (FluA), influenza B virus (FluB), respiratory syncytial virus (RSV), human parainfluenza virus (hPIV), mycoplasma pneumoniae (MP), novel coronavirus (SARS-CoV-2), human respiratory adenovirus (HADV), human respiratory nose / enterovirus (HRV / HEV), bordetella pertussis (BP), human coronavirus (HCoV), human bocavirus (HBoV) and chlamydia pneumoniae (CP), PCR (polymerase chain reaction) freeze-dried powder and a buffer solution. According to the kit, primer system optimization is carried out on various pathogens, brand new primers and probe pools are designed, clinical sensitivity and specificity can be achieved, and meanwhile, the kit is matched with a fully-closed full-automatic detection card box integrating sample treatment and amplification and is suitable for rapid detection of respiratory tract pathogens.

The invention relates to a composition and a kit for detecting chlamydia pneumonia, mycoplasma pneumonia and respiratory adenovirus, the composition comprises a primer pair and a probe corresponding to the primer pair, the primer pair comprises a primer pair 1 aiming at the chlamydia pneumonia, a primer pair 2 aiming at the mycoplasma pneumonia and a primer pair 3 aiming at the respiratory adenovirus; the probes comprise a specific probe 1 aiming at chlamydia pneumonia, a specific probe 2 aiming at mycoplasma pneumonia and a specific probe 3 aiming at respiratory adenovirus. According to the invention, the optimal primer pair and probe combination is screened out from the designed six groups of specific primer pairs and probes, so that the requirement of high sensitivity is met, the detection result is highly accurate, the detection sensitivity can reach 1copies / reaction, and the anti-interference capability is relatively strong. The PCR amplification reagent in the kit is presented in the form of a freeze-dried product, can be stored at room temperature, and has stable performance within 12 months, and the detection sensitivity and precision meet the detection requirements.

The invention discloses a kit for rapidly detecting seven genital tract pathogens by a one-tube method. The kit comprises a primer and a probe aiming at ureaplasma urealyticum, a primer and a probe aiming at ureaplasma parvum, a primer and a probe aiming at chlamydia trachomatis, a primer and a probe aiming at gonococcus, a primer and a probe aiming at mycoplasma genitalium, a primer and a probe aiming at mycoplasma hominis and a primer and a probe aiming at trichomonas vaginalis. In addition, the invention also discloses a method for rapidly detecting seven common genital tract pathogens by a one-tube method. According to the method, the detection sensitivity is greatly improved, the operation is simple, and seven genital tract pathogens can be simultaneously detected in a short time.

The application provides a primer probe composition and application thereof in detection of urogenital tract pathogens, and relates to the technical field of biology. The primer probe composition comprises a chlamydia trachomatis primer probe pair with sequences shown in SEQ ID NO:1-6; a gonococcus primer probe pair with sequences shown in SEQ ID NO:7-9; and a mycoplasma genitalium primer probe pair with sequences shown in SEQ ID NO:10-12. The primer probe pairs in the primer probe composition do not have significant cross interference, the lower limit of detection can be as low as 20 copies / reaction, 200 copies / mL, and the problem that the lower limit of detection of the urogenital tract pathogens in the prior art needs to be reduced is solved.

Described herein are compositions and methods for treating or preventing skin or ocular surface disorders, such as those associated with bacterial and / or parasitic infections. Bacterial and / or parasitic infections include mites (e.g., Demodex), helminths, protozoa (e.g., amebas (e.g., Acanthamoeba or Cryptosporidiosis)), onchocerciasis, lice, scabies, nematodes, or chlamydia. The compositions and methods include compounds that exhibit immunological activity, keratolytic activity, antihistamine activity, anti-inflammatory activity, and / or other desirable activities. Topical administration of the compositions to the eyelid margin or surrounding area provides therapeutic benefit to patients with ocular surface disorders.

The present invention relates to a pyrazine macrolide compound and the use thereof. Specifically disclosed is a compound as represented by formula I or a pharmaceutically acceptable salt thereof. The compound of the present invention exhibits activity against bacteria, mycoplasmas, or chlamydias, and is useful for preventing and / or treating diseases caused by bacteria, mycoplasmas, or chlamydias. Further, the compound of the present application can achieve an antibacterial activity comparable to or better than that of azithromycin or solithromycin at a lower dosage. Furthermore, the compound of the present application achieves a superior antibacterial effect on Gram-positive bacteria. Still furthermore, the compound of the present application exhibits a better inhibitory activity against mycoplasmas and a lower hepatotoxicity.

The invention relates to the technical field of molecular biology, and discloses an LAMP (loop-mediated isothermal amplification) primer group and a kit for detecting three items (Uu, CT and NG) of venereal diseases. The LAMP primer group disclosed by the invention comprises a first primer group for targeting a ureaplasma urealyticum UreB gene, a second primer group for targeting a chlamydia trachomatis gyrA gene and a third primer group for targeting a gonococcus glnA gene. The LAMP primer group provided by the invention has good thermal stability, is suitable for an LAMP amplification detection system at a high temperature of 70 DEG C, reduces non-specific binding, and has good detection sensitivity and specificity. The LAMP primer group and the kit provided by the invention provide an innovative solution for accurate diagnosis of sexually transmitted diseases.

The present application belongs to the field of molecular biology detection, and particularly relates to a composition, method and use for simultaneous detection and differentiation of swine fever virus, psittacosis chlamydia, porcine reproductive and respiratory syndrome virus and porcine circovirus type II. The composition for simultaneous detection provided by the present application can realize rapid detection of four pathogen target genes in one tube of reaction solution by using multiplex fluorescence RPA detection technology, and has the advantages of high throughput, short time and low cost. On one hand, the present application can accurately detect pathogens and analyze mixed infection of pathogens, so as to achieve the effect of precise medication, reduce the use of antibiotics, save costs, and reduce the cost and increase the benefit of enterprises, and on the other hand, compared with fluorescence PCR, the detection time is greatly shortened, and the purpose of rapid detection is achieved.

The present application relates to the field of biochemistry, and discloses a multiple primer set and a kit for detecting pathogens of acute infection of the lower respiratory tract, including primer pairs for detecting Mycoplasma pneumoniae, primer pairs for detecting Chlamydia pneumoniae, primer pairs for detecting Staphylococcus aureus, primer pairs for detecting Haemophilus influenzae, primer pairs for detecting Streptococcus pneumoniae, and primer pairs for detecting Klebsiella pneumoniae. The multiple primer set for detecting pathogens of acute infection of the lower respiratory tract of the present application can realize simultaneous amplification and detection of 6 targets, and is suitable for screening of clinical cases of acute lower respiratory tract infection.

The application discloses a parrot-origin reovirus RT-qPCR specific detection primer and a detection method. The primer probe composition takes a S1 gene conservative region of the parrot-origin reovirus as an amplification target, and comprises a specific upstream primer, a downstream primer and a fluorescent probe. The nucleotide sequence of the upstream primer is shown as SEQ ID NO:1, the nucleotide sequence of the downstream primer PSRV-S1-R is shown as SEQ ID NO:2, and the nucleotide sequence of the fluorescent probe PSRV-S1-Probe is shown as SEQ ID NO:3. The application designs specific primers and a TaqMan probe for the parrot-origin reovirus, has an amplification signal for the PSRV, has no cross reaction with common pathogens such as APV, BFDV, FAdV, ARV, NDRV and parrot fever chlamydia, has good specificity, is accurate in quantification, has good repeatability and stability, and is simple in operation and high in flux.

The present invention belongs to the technical field of immunology, and specifically relates to a subunit vaccine for animal abortion chlamydia, and a preparation method and application thereof. The subunit vaccine of the present invention comprises a chlamydia antigen and an adjuvant; the chlamydia antigen comprises one or more combinations of MOMP, OmcB, incA and CPAF. The subunit vaccine of the present invention has good immune effect, high safety, can produce a high level of antibodies, and can prevent and / or treat diseases caused by chlamydia, such as abortion.

The invention discloses an acetylisovaleryltylosin tartrate derivative shown in a general formula I or a pharmaceutically acceptable salt thereof, and preparation and anti-infection application thereof. The compound not only can inhibit infection of bacteria, mycoplasma and chlamydia, but also has the effects of inhibiting PRRSV virus replication and reducing virus titer, and can be used for preventing and treating PRRSV in the pig breeding industry and reducing the morbidity and case fatality rate of PRRSV.

The present invention describes an efficient vaccine against a Chlamydia trachomatis (Ct). The vaccine is based on recombinant fusion molecules that are capable of generating a high titered neutralizing antibody response that is protective against various Ct serovars. Our invention furthermore describe the combination of these antibody promoting fragments with Ct antigens that are targets for T cells with the aim to provide a vaccine that activate both arms of the immune system.

The invention relates to the technical field of pathogenic microorganism infection detection, and provides a respiratory flora marker for assisting in predicting chlamydia psittaci infection and application of the respiratory flora marker. The respiratory tract flora markers comprise Candida glabrata, Lactobacillus paracasei, and Lactobacillus rhamnosus, and the respiratory tract flora markers comprise Candida glabrata, Lactobacillus paracasei, Lactobacillus rhamnosus, Lactobacillus paracasei, Lactobacillus rhamnosus, Lactobacillus paracasei, Lactobacillus rhamnosus, Lactobacillus paracasei, Lactobacillus paracasei, and Lactobacillus rhamnosus. The respiratory flora marker for aided prediction of chlamydia psittaci infection is applied to preparation of a kit for aided diagnosis of severe pneumonia caused by chlamydia psittaci infection, has high sensitivity and high specificity, reduces misdiagnosis and missed diagnosis, can effectively evaluate the risk of severe illness of a patient suffering from chlamydia psittaci infection, and has a good application prospect in the treatment of severe pneumonia caused by chlamydia psittaci infection. Therefore, medical intervention can be carried out as soon as possible, and great clinical application value is achieved.

Contemplated compositions, devices, and methods comprise immunodominant antigens from selected human pathogens (Burkholderia pseudomallei, Borrelia burgdorferi, Brucella melitensis, Chlamydia muridarum, Coxiella burnetii, Francisella tularensis, human Herpes virus 1 and 2, Mycobacterium tuberculosis, Plasmodium falciparum, and Vaccinia virus) can be used as a vaccine, as diagnostic markers, and as therapeutic agents. In particularly preferred aspects, the antigens have quantified and known relative reactivities with respect to sera of a population infected with the pathogen, and have a known association with a disease parameter.

Disclosed are the H. zea nudivirus sequence, two additional related sequences from H. virescens and H. armigera nudiviruses for development of genetically modified nudiviruses capable of being sexually transmitted by an insect useful for controlling pest populations, methods for preparing egg populations for controlling pest populations and methods for controlling pest populations utilizing such egg populations. Such genetically modified nudiviruses are capable of causing sterility in a target population of insects. Previously disclosed were insects infected with the previously disclosed genetically modified H. zea nudivirus, methods of making genetically modified nudiviruses, and methods of using genetically modified nudiviruses to control an insect pest population.

The present disclosure provides a set of primers and optional probes for identifying the presence of α-coronavirus, β-coronavirus, SARS-CoV-2, adenovirus, Chlamydia pneumoniae, Influenza A, Influenza B, metapneumovirus, rhinovirus / enterovirus, mycoplasma, Bordetella spp., parainfluenza, and respiratory syncytial virus (RSV), which can be included with (e.g., in at kit) or in a cartridge for automated detection of these pathogens by nucleic acid amplification. This disclosure also provides related detection methods, as well as cartridges, systems, and kits useful in such methods.

The invention relates to the technical field of molecular biological detection. The invention provides a primer pair and a probe for detecting chlamydia abortus, and further provides a kit which comprises the primer pair and the probe and is used for quantitatively detecting the chlamydia abortus through micro-droplet digital PCR, and a non-diagnostic method for quantitatively detecting the chlamydia abortus based on the micro-droplet digital PCR. And negative and positive results are judged according to the fluorescence signal condition of the microdroplet in the amplification product. According to the technical scheme, rapid and effective identification of the chlamydia abortus disease can be achieved, cross reaction with other common pathogens can be avoided while the chlamydia abortus is detected, and the method has good specificity, good repeatability and high sensitivity.

This invention discloses a multiplex qPCR kit for the simultaneous detection of six respiratory pathogens. The multiplex qPCR kit of this invention can simultaneously detect six common respiratory pathogens: adenovirus, human bocavirus, respiratory syncytial virus, mycoplasma pneumoniae, rhinovirus, and chlamydia pneumoniae. It has the advantages of high specificity, high sensitivity, good repeatability, and is unaffected by interfering substances. Furthermore, the kit is simple to operate and quick to use for detecting adenovirus, human bocavirus, respiratory syncytial virus, mycoplasma pneumoniae, rhinovirus, or chlamydia pneumoniae. It can simultaneously detect these six different common respiratory pathogens using a two-tube reaction system, and the detection results are accurate. It is suitable for rapid clinical detection of respiratory pathogens, which helps in timely and accurate treatment of the infecting pathogens.

Vaccine compositions comprising a plurality of immunogens attached to a carrier for inducing an immune response against a pathogen selected from the Anaplasma, Babesia, Borrelia, and Chlamydia genera. Vaccine compositions comprising multivalent carriers and related methods using the vaccine compositions in various therapeutic and prophylactic applications for inducing an immune response against, treating, or preventing an infection caused by the Anaplasma, Babesia, Borrelia, or Chlamydia pathogens, and the related diseases thereof.

Provided is the determination of the three-dimensional structure of Chlamydia major outer membrane protein and applications and uses thereof, including for vaccine development, drug development, and diagnostics.

The invention is applicable to the technical field of biological detection, and provides a primer probe group, system and method for simultaneously detecting mycoplasma pneumoniae and chlamydia pneumoniae, specific primers and probes are designed aiming at conserved regions of 16S rRNA gene of MP and MOMP gene of CP, the two probes are respectively marked with FAM and ROX fluorophores, and the specific primers and probes can be used for simultaneously detecting mycoplasma pneumoniae and chlamydia pneumoniae. Parallel detection of MP and CP nucleic acids can be realized in a single reaction tube. The detection system provided by the invention comprises the PCR enhancer, so that the detection sensitivity and the amplification efficiency are effectively improved. The method further comprises an optimized nucleic acid extraction step and a reaction procedure, has the advantages of being easy and convenient to operate, rapid in detection, high in sensitivity and high in specificity and is suitable for early-stage rapid diagnosis of clinical respiratory tract samples.

The invention discloses a primer probe composition, a kit and a method for detecting 16 pathogenic microorganisms. The 16 pathogenic microorganisms comprise total bacterial colonies, total vibrio, helicobacter pylori, aeromonas hydrophila, mycobacterium tuberculosis, vibrio cholerae, yersinia pestis, pseudomonas aeruginosa, staphylococcus aureus, mycoplasma, chlamydia, rickettsia and toxoplasma gondii. The sequences of screened primer probes are shown as SEQ ID NO.1-SEQ ID NO.48, the sensitivity is highest, the specificity is good, the primer probes serve as a quadruple detection reagent, an optimized lysis solution and a fluorescent PCR amplification program are combined, the extraction effect and the sensitivity are guaranteed, the method is combined with a nucleic acid extraction and detection integrated device, the detection efficiency is high, and the detection cost is low. Full automation is achieved from nucleic acid extraction to qPCR detection, only 60 min is needed from extraction to qPCR detection, and the detection time is shortened.

The present invention relates to an aminopyridone macrolide compound and the use thereof. Specifically disclosed is a compound represented by formula I or a pharmaceutically acceptable salt thereof. The compound of the present invention exhibits antibacterial, antimycoplasmal or antichlamydial activity, and can be used for preventing and / or treating related diseases caused by bacteria, mycoplasmas or chlamydias. Further, the compound of the present application can achieve an antibacterial activity comparable to or better than that of azithromycin or solithromycin at a lower dosage. Still further, the compound of the present application exhibits a superior antibacterial effect on Gram-positive bacteria. Even further, the compound of the present application exhibits a better inhibitory activity against mycoplasmas and a lower hepatotoxicity.

The present application relates to the technical field of biological detection, in particular to a composition and application thereof. The present application provides a composition and application thereof in a process of coating a magnetic bead with a natural antigen of Chlamydia pneumoniae, and a Chlamydia pneumoniae IgG antibody detection kit (magnetic microparticle chemiluminescence method). In the process, a surfactant and a reducing agent are added in the antigen coating process to improve the contrast of the detection results, a heat sealing process for 3 days is adopted to reduce the result range between different samples, and CB alkaline buffer is used as a sealing liquid buffer to improve the aggregation problem of the magnetic beads.

Described herein are compositions and methods for the treatment or prevention of dermal or ocular surface disorders, such as those associated with bacterial and / or parasitic infections. Bacterial and / or parasitic infections include mites (e.g., demodex), worms, protozoa (e.g., amoeba (e.g., acanthamoeba or cryptosporidiosis)), onchocerciasis, lice, scabies, nematodes, or chlamydia. Said compositions and methods comprise compounds which demonstrate immunological, keratolytic, anti-histamine, anti-inflammatory, and / or other desirable activities. Topical administration of said compositions to the eyelid margin or surrounding areas provides therapeutic benefit to patients suffering from ocular surface disorders.

The present disclosure provides a set of primers and optional probes for identifying the presence of alpha-coronavirus, beta-coronavirus, SARS-CoV-2, adenovirus, chlamydia pneumoniae, influenza A, influenza B, metapneumovirus, rhinovirus / enterovirus, mycoplasma, Bordetella, parainfluenza, and respiratory syncytial virus (RSV), the present invention relates to nucleic acid probes, which may be included with a cartridge (e.g., in a kit) or in a cartridge, for automated detection of these pathogens by nucleic acid amplification. The disclosure also provides related detection methods, as well as kits, systems, and kits useful in such methods.

The present application discloses a Pickering emulsion, its preparation method and application. The Pickering emulsion comprises an oil-in-water emulsion composed of graphene oxide quantum dots, an oil phase and an aqueous phase. The preparation method comprises the following steps: mixing a graphene oxide quantum dot solution, an oil phase and water to obtain a mixed solution, and emulsifying the mixed solution under ultrasonic conditions to obtain the Pickering emulsion. The Pickering emulsion uses graphene oxide quantum dots as an emulsifier. Due to the high surface area, biodegradability and biocompatibility of the graphene oxide quantum dots, the Pickering emulsion shows a high immune enhancement efficiency, can slowly release antigens in vivo and in vitro, has biocompatibility, and does not cause damage to the heart, liver and kidney functions of mice. It can be used as a candidate adjuvant with good immune enhancement and biocompatibility. The preparation method of the Pickering emulsion in the present invention is simple and easy to implement, and has low cost. The present invention also discloses the application of the above Pickering emulsion as an adjuvant for a Chlamydia trachomatis vaccine.

The present invention describes an efficient vaccine against a Chlamydia trachomatis (Ct). The vaccine is based on recombinant fusion molecules that are capable of generating a high titered neutralizing antibody response that is protective against various Ct serovars. Our invention furthermore describe the combination of these antibody promoting fragments with Ct antigens that are targets for T cells with the aim to provide a vaccine that activate both arms of the immune system.

HIV pre-exposure prophylaxis (PrEP) has been available for nearly a decade. Patients can take a pill comprising of multiple antiviral agents that, if exposed to HIV, will prevent a permanent viral infection with an extremely high success rate. However, the use of PrEP antivirals has been linked to higher rates of STIs including HSV-1, HSV-2, Infectious Mononucleosis, chlamydia, gonorrhea, trichomanias, and syphilis. In particular, Herpes Simplex Viruses 1 and 2 can cause symptoms including oral or genital sores, while mono, caused by the Epstein-Barr Virus, can cause prolonged sickness. This disclosure utilizes two or more antiviral agents as a multi-use therapy for both suppressing a chronic herpesvirus infection while preventing an HIV infection. With this therapy, the odds of both acquiring HIV or spreading a herpesvirus are greatly reduced during human contact.