37 results about "Chlamydia" patented technology

The application provides a primer probe composition and application thereof in detection of urogenital tract pathogens, and relates to the technical field of biology. The primer probe composition comprises a chlamydia trachomatis primer probe pair with sequences shown in SEQ ID NO:1-6; a gonococcus primer probe pair with sequences shown in SEQ ID NO:7-9; and a mycoplasma genitalium primer probe pair with sequences shown in SEQ ID NO:10-12. The primer probe pairs in the primer probe composition do not have significant cross interference, the lower limit of detection can be as low as 20 copies / reaction, 200 copies / mL, and the problem that the lower limit of detection of the urogenital tract pathogens in the prior art needs to be reduced is solved.

Described herein are compositions and methods for treating or preventing skin or ocular surface disorders, such as those associated with bacterial and / or parasitic infections. Bacterial and / or parasitic infections include mites (e.g., Demodex), helminths, protozoa (e.g., amebas (e.g., Acanthamoeba or Cryptosporidiosis)), onchocerciasis, lice, scabies, nematodes, or chlamydia. The compositions and methods include compounds that exhibit immunological activity, keratolytic activity, antihistamine activity, anti-inflammatory activity, and / or other desirable activities. Topical administration of the compositions to the eyelid margin or surrounding area provides therapeutic benefit to patients with ocular surface disorders.

The present invention relates to a pyrazine macrolide compound and the use thereof. Specifically disclosed is a compound as represented by formula I or a pharmaceutically acceptable salt thereof. The compound of the present invention exhibits activity against bacteria, mycoplasmas, or chlamydias, and is useful for preventing and / or treating diseases caused by bacteria, mycoplasmas, or chlamydias. Further, the compound of the present application can achieve an antibacterial activity comparable to or better than that of azithromycin or solithromycin at a lower dosage. Furthermore, the compound of the present application achieves a superior antibacterial effect on Gram-positive bacteria. Still furthermore, the compound of the present application exhibits a better inhibitory activity against mycoplasmas and a lower hepatotoxicity.

The invention relates to the technical field of molecular biology, and discloses an LAMP (loop-mediated isothermal amplification) primer group and a kit for detecting three items (Uu, CT and NG) of venereal diseases. The LAMP primer group disclosed by the invention comprises a first primer group for targeting a ureaplasma urealyticum UreB gene, a second primer group for targeting a chlamydia trachomatis gyrA gene and a third primer group for targeting a gonococcus glnA gene. The LAMP primer group provided by the invention has good thermal stability, is suitable for an LAMP amplification detection system at a high temperature of 70 DEG C, reduces non-specific binding, and has good detection sensitivity and specificity. The LAMP primer group and the kit provided by the invention provide an innovative solution for accurate diagnosis of sexually transmitted diseases.

The present application belongs to the field of molecular biology detection, and particularly relates to a composition, method and use for simultaneous detection and differentiation of swine fever virus, psittacosis chlamydia, porcine reproductive and respiratory syndrome virus and porcine circovirus type II. The composition for simultaneous detection provided by the present application can realize rapid detection of four pathogen target genes in one tube of reaction solution by using multiplex fluorescence RPA detection technology, and has the advantages of high throughput, short time and low cost. On one hand, the present application can accurately detect pathogens and analyze mixed infection of pathogens, so as to achieve the effect of precise medication, reduce the use of antibiotics, save costs, and reduce the cost and increase the benefit of enterprises, and on the other hand, compared with fluorescence PCR, the detection time is greatly shortened, and the purpose of rapid detection is achieved.

The application discloses a parrot-origin reovirus RT-qPCR specific detection primer and a detection method. The primer probe composition takes a S1 gene conservative region of the parrot-origin reovirus as an amplification target, and comprises a specific upstream primer, a downstream primer and a fluorescent probe. The nucleotide sequence of the upstream primer is shown as SEQ ID NO:1, the nucleotide sequence of the downstream primer PSRV-S1-R is shown as SEQ ID NO:2, and the nucleotide sequence of the fluorescent probe PSRV-S1-Probe is shown as SEQ ID NO:3. The application designs specific primers and a TaqMan probe for the parrot-origin reovirus, has an amplification signal for the PSRV, has no cross reaction with common pathogens such as APV, BFDV, FAdV, ARV, NDRV and parrot fever chlamydia, has good specificity, is accurate in quantification, has good repeatability and stability, and is simple in operation and high in flux.

The invention discloses an acetylisovaleryltylosin tartrate derivative shown in a general formula I or a pharmaceutically acceptable salt thereof, and preparation and anti-infection application thereof. The compound not only can inhibit infection of bacteria, mycoplasma and chlamydia, but also has the effects of inhibiting PRRSV virus replication and reducing virus titer, and can be used for preventing and treating PRRSV in the pig breeding industry and reducing the morbidity and case fatality rate of PRRSV.

The present invention describes an efficient vaccine against a Chlamydia trachomatis (Ct). The vaccine is based on recombinant fusion molecules that are capable of generating a high titered neutralizing antibody response that is protective against various Ct serovars. Our invention furthermore describe the combination of these antibody promoting fragments with Ct antigens that are targets for T cells with the aim to provide a vaccine that activate both arms of the immune system.

The invention relates to the technical field of pathogenic microorganism infection detection, and provides a respiratory flora marker for assisting in predicting chlamydia psittaci infection and application of the respiratory flora marker. The respiratory tract flora markers comprise Candida glabrata, Lactobacillus paracasei, and Lactobacillus rhamnosus, and the respiratory tract flora markers comprise Candida glabrata, Lactobacillus paracasei, Lactobacillus rhamnosus, Lactobacillus paracasei, Lactobacillus rhamnosus, Lactobacillus paracasei, Lactobacillus rhamnosus, Lactobacillus paracasei, Lactobacillus paracasei, and Lactobacillus rhamnosus. The respiratory flora marker for aided prediction of chlamydia psittaci infection is applied to preparation of a kit for aided diagnosis of severe pneumonia caused by chlamydia psittaci infection, has high sensitivity and high specificity, reduces misdiagnosis and missed diagnosis, can effectively evaluate the risk of severe illness of a patient suffering from chlamydia psittaci infection, and has a good application prospect in the treatment of severe pneumonia caused by chlamydia psittaci infection. Therefore, medical intervention can be carried out as soon as possible, and great clinical application value is achieved.

Contemplated compositions, devices, and methods comprise immunodominant antigens from selected human pathogens (Burkholderia pseudomallei, Borrelia burgdorferi, Brucella melitensis, Chlamydia muridarum, Coxiella burnetii, Francisella tularensis, human Herpes virus 1 and 2, Mycobacterium tuberculosis, Plasmodium falciparum, and Vaccinia virus) can be used as a vaccine, as diagnostic markers, and as therapeutic agents. In particularly preferred aspects, the antigens have quantified and known relative reactivities with respect to sera of a population infected with the pathogen, and have a known association with a disease parameter.

Disclosed are the H. zea nudivirus sequence, two additional related sequences from H. virescens and H. armigera nudiviruses for development of genetically modified nudiviruses capable of being sexually transmitted by an insect useful for controlling pest populations, methods for preparing egg populations for controlling pest populations and methods for controlling pest populations utilizing such egg populations. Such genetically modified nudiviruses are capable of causing sterility in a target population of insects. Previously disclosed were insects infected with the previously disclosed genetically modified H. zea nudivirus, methods of making genetically modified nudiviruses, and methods of using genetically modified nudiviruses to control an insect pest population.

This invention discloses a multiplex qPCR kit for the simultaneous detection of six respiratory pathogens. The multiplex qPCR kit of this invention can simultaneously detect six common respiratory pathogens: adenovirus, human bocavirus, respiratory syncytial virus, mycoplasma pneumoniae, rhinovirus, and chlamydia pneumoniae. It has the advantages of high specificity, high sensitivity, good repeatability, and is unaffected by interfering substances. Furthermore, the kit is simple to operate and quick to use for detecting adenovirus, human bocavirus, respiratory syncytial virus, mycoplasma pneumoniae, rhinovirus, or chlamydia pneumoniae. It can simultaneously detect these six different common respiratory pathogens using a two-tube reaction system, and the detection results are accurate. It is suitable for rapid clinical detection of respiratory pathogens, which helps in timely and accurate treatment of the infecting pathogens.

Vaccine compositions comprising a plurality of immunogens attached to a carrier for inducing an immune response against a pathogen selected from the Anaplasma, Babesia, Borrelia, and Chlamydia genera. Vaccine compositions comprising multivalent carriers and related methods using the vaccine compositions in various therapeutic and prophylactic applications for inducing an immune response against, treating, or preventing an infection caused by the Anaplasma, Babesia, Borrelia, or Chlamydia pathogens, and the related diseases thereof.

Provided is the determination of the three-dimensional structure of Chlamydia major outer membrane protein and applications and uses thereof, including for vaccine development, drug development, and diagnostics.

The invention is applicable to the technical field of biological detection, and provides a primer probe group, system and method for simultaneously detecting mycoplasma pneumoniae and chlamydia pneumoniae, specific primers and probes are designed aiming at conserved regions of 16S rRNA gene of MP and MOMP gene of CP, the two probes are respectively marked with FAM and ROX fluorophores, and the specific primers and probes can be used for simultaneously detecting mycoplasma pneumoniae and chlamydia pneumoniae. Parallel detection of MP and CP nucleic acids can be realized in a single reaction tube. The detection system provided by the invention comprises the PCR enhancer, so that the detection sensitivity and the amplification efficiency are effectively improved. The method further comprises an optimized nucleic acid extraction step and a reaction procedure, has the advantages of being easy and convenient to operate, rapid in detection, high in sensitivity and high in specificity and is suitable for early-stage rapid diagnosis of clinical respiratory tract samples.

The present invention relates to an aminopyridone macrolide compound and the use thereof. Specifically disclosed is a compound represented by formula I or a pharmaceutically acceptable salt thereof. The compound of the present invention exhibits antibacterial, antimycoplasmal or antichlamydial activity, and can be used for preventing and / or treating related diseases caused by bacteria, mycoplasmas or chlamydias. Further, the compound of the present application can achieve an antibacterial activity comparable to or better than that of azithromycin or solithromycin at a lower dosage. Still further, the compound of the present application exhibits a superior antibacterial effect on Gram-positive bacteria. Even further, the compound of the present application exhibits a better inhibitory activity against mycoplasmas and a lower hepatotoxicity.

The present application relates to the technical field of biological detection, in particular to a composition and application thereof. The present application provides a composition and application thereof in a process of coating a magnetic bead with a natural antigen of Chlamydia pneumoniae, and a Chlamydia pneumoniae IgG antibody detection kit (magnetic microparticle chemiluminescence method). In the process, a surfactant and a reducing agent are added in the antigen coating process to improve the contrast of the detection results, a heat sealing process for 3 days is adopted to reduce the result range between different samples, and CB alkaline buffer is used as a sealing liquid buffer to improve the aggregation problem of the magnetic beads.

Described herein are compositions and methods for the treatment or prevention of dermal or ocular surface disorders, such as those associated with bacterial and / or parasitic infections. Bacterial and / or parasitic infections include mites (e.g., demodex), worms, protozoa (e.g., amoeba (e.g., acanthamoeba or cryptosporidiosis)), onchocerciasis, lice, scabies, nematodes, or chlamydia. Said compositions and methods comprise compounds which demonstrate immunological, keratolytic, anti-histamine, anti-inflammatory, and / or other desirable activities. Topical administration of said compositions to the eyelid margin or surrounding areas provides therapeutic benefit to patients suffering from ocular surface disorders.

The present disclosure provides a set of primers and optional probes for identifying the presence of alpha-coronavirus, beta-coronavirus, SARS-CoV-2, adenovirus, chlamydia pneumoniae, influenza A, influenza B, metapneumovirus, rhinovirus / enterovirus, mycoplasma, Bordetella, parainfluenza, and respiratory syncytial virus (RSV), the present invention relates to nucleic acid probes, which may be included with a cartridge (e.g., in a kit) or in a cartridge, for automated detection of these pathogens by nucleic acid amplification. The disclosure also provides related detection methods, as well as kits, systems, and kits useful in such methods.

The present invention describes an efficient vaccine against a Chlamydia trachomatis (Ct). The vaccine is based on recombinant fusion molecules that are capable of generating a high titered neutralizing antibody response that is protective against various Ct serovars. Our invention furthermore describe the combination of these antibody promoting fragments with Ct antigens that are targets for T cells with the aim to provide a vaccine that activate both arms of the immune system.

The present invention provides novel probes for use in LAMP detection methods. The probe comprises a single fluorophore label bound to an internal cytosine residue of the probe. The probes are particularly useful in the detection of Chlamydia and gonorrhea infections in patients.

The invention relates to a pyridone macrolide compound and application thereof. Specifically disclosed is a compound represented by formula I-1 or I-1 'or a pharmaceutically acceptable salt thereof. The compound disclosed by the invention has the activity of resisting bacteria, mycoplasma or chlamydia, and can be used for preventing and / or treating related diseases caused by bacteria, mycoplasma or chlamydia; furthermore, the antibacterial activity equivalent to or better than that of azithromycin or solificin can be achieved by using a lower dosage of the compound provided by the invention; furthermore, the compound provided by the invention has a better antibacterial effect on gram-positive bacteria; furthermore, the compound provided by the invention has better inhibitory activity and lower hepatotoxicity to mycoplasma.

Provided is the determination of the three-dimensional structure of Chlamydia major outer membrane protein and applications and uses thereof, including for vaccine development, drug development, and diagnostics.

The invention provides a primer group and a kit for detecting four bacterial abortion diseases of sheep, belongs to the technical field of biological detection, establishes a multiplex PCR (polymerase chain reaction) detection method for sheep brucellosis, sheep abortion salmonellosis, listeriosis and chlamydia disease and a kit thereof, and has the characteristics of high sensitivity, strong specificity, simplicity and convenience in operation, short time consumption and the like. The rapid differential diagnosis of four sheep bacterial abortion diseases can be realized through one-time PCR amplification, and the method has important advantages in the field of sheep disease diagnosis and identification.

The invention discloses a cervical cancer plasma circulating microbial marker combination, a reagent, a kit, a risk assessment model and application thereof, and belongs to the cross technical field of liquid biopsy and microbiomics. The cervical cancer plasma circulating microbial marker combination disclosed by the invention is prepared from prevotella, stenosiella, chlamydia trachomatis, peptostreptococcus, fusobacterium, campylobacter and haemophilus. Cervical cancer patients and healthy people are verified by using the cervical cancer plasma circulating microbial marker combination disclosed by the invention, the AUC value is 0.939, the sensitivity is 81.58%, the specificity is 90.00%, the accuracy is 85.29%, the positive predictive value is 91.18%, and the negative predictive value is 79.41%, so that the cervical cancer plasma circulating microbial marker combination disclosed by the invention can be used for effectively distinguishing the cervical cancer patients from the healthy people; the method is suitable for early screening of cervical cancer and has remarkable clinical screening application value.

The invention discloses a method for researching the relation between intestinal colonization and oviduct edema after chlamydia genital tract infection and application, and belongs to the technical field of biological medicine. The invention provides a complete and standardized research system which can be used for researching the relationship between intestinal colonization and oviduct edema after chlamydia genital tract infection; the provided method reveals for the first time that chlamydia genital tract infection is colonized in intestinal tracts and has an inevitable relation with the formation of lasting and stable oedema of fallopian tubes, and a new direction is provided for prevention and treatment of oedema of fallopian tubes induced by chlamydia genital tract infection; therefore, the method provided by the invention can be used for researching the relationship between chlamydia intestinal colonization and oviduct edema, and also has a reference value for other similar researches; the invention also provides a group of preparations which can be used for diagnosis and / or risk prediction and / or prevention and / or treatment of fallopian tube edema induced by chlamydia genital tract infection, and the preparations are symbolic flora obtained by identification with the method provided by the invention.

Described herein are compositions and methods for the treatment or prevention of dermal or ocular surface disorders, such as those associated with bacterial and / or parasitic infections. Bacterial and / or parasitic infections include mites (e.g., demodex), worms, protozoa (e.g., amoeba (e.g., acanthamoeba or cryptosporidiosis)), onchocerciasis, lice, scabies, nematodes, or chlamydia. Said compositions and methods comprise compounds which demonstrate immunological, keratolytic, anti-histamine, anti-inflammatory, and / or other desirable activities. Topical administration of said compositions to the eyelid margin or surrounding areas provides therapeutic benefit to patients suffering from ocular surface disorders.

The invention provides a mycoplasma pneumoniae / chlamydia combined detection method, which efficiently enriches target genes through cascade reaction of RPA (recombinase polymerase amplification) pre-amplification (37 DEG C + / -1 DEG C, 5 minutes) and LAMP (loop-mediated isothermal amplification) targeted amplification (40 DEG C + / -2 DEG C, 15 minutes). A dUTP / UDG anti-pollution system and a closed tube operation process are adopted, an amplification product is directly mixed with a double-labeled colloidal gold probe, and double pathogens are synchronously detected through an immunochromatography test strip. The test strip is provided with two independent detection lines, FAM and digoxin reporter molecules are captured respectively, detection is completed within 25 minutes, sensitivity is high, cross contamination is avoided, and the test strip is suitable for rapid diagnosis of basic respiratory pathogens.

Using a novel dependence on plasmid-mediated expression (DOPE) technology, conditional depletion of GrgA, a Chlamydia-specific protein, has been demonstrated to result in greatly reduced reticulate body (RB) proliferation rate and near complete lack of elementary body (EB) formation, thus disrupting the normal chlamydial developmental cycle. This conditional GrgA-deficient Chlamydia allows study of chlamydial growth and developmental regulation and can be used as the basis of an attenuated, maturation-defective but immunogenic bacteria for use as an avirulent vaccine against Chlamydia.