Exfoliative cells preserving fluid

A technology of exfoliated cells and preservation solution, which is applied in the field of exfoliated cell preservation solution, can solve the problems of unsatisfactory treatment, short storage time of cells, unsatisfactory treatment of specimen impurities, etc., and achieve the effect of good transparency, easy promotion, and intact cell shape

Inactive Publication Date: 2013-05-29
刘召宏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are also some subsequent patents for cell preservation solutions, but they all have defects such as short cell storage time, unsatisf

Method used

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  • Exfoliative cells preserving fluid
  • Exfoliative cells preserving fluid
  • Exfoliative cells preserving fluid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: The cells used in this example are cervical exfoliated cells, and the preparation method is centrifugal sedimentation method. The ratio of the exfoliated cell preservation solution in this example is as follows:

[0027] Components in weight percentage 0.7% sodium chloride, 0.25% trisodium phosphate, 7.5% EDTA di-sodium, EDTA dipotassium, one of heparin, 1% penicillin, 3% paraformaldehyde, 3% coumarin, 0.1% methylcysteine, 0.3% glacial acetic acid, 2.5% carbolic acid, 0.2% Tween-20.

[0028] Components in percent by volume: 60% pH buffer (pH7), 16% absolute ethanol, 12.5% ​​methanol, 3% acetone, wherein the pH buffer is one of PBS, Tris-HCl, and PIPES.

Embodiment 2

[0029] Embodiment 2: The cells used in this example are exfoliated cells of the cervix, and the preparation method is a membrane filtration method. The ratio of the exfoliated cell preservation solution in this embodiment is as follows:

[0030] Components in weight percent: 0.9% sodium chloride, 0.1% trisodium phosphate, 10% edetate disodium, edetate dipotassium, one of heparin, 1% streptomycin, 4% more Polyoxymethylene, 4% coumarin, 0.5% dithiothreitol, 0.5% bromo-16 alkyltrimethylamine, 1% glycerol, 0.5% octadecyltrimethylammonium chloride.

[0031] Components in volume percentage: 50% pH buffer (pH6.8), 20% absolute ethanol, 15% methanol, 4% acetone, wherein the pH buffer is one of PBS, Tris-HCl, and PIPES.

Embodiment 3

[0032] Embodiment 3: The cells used in this example are exfoliated cells of the cervix, and the preparation method is the natural sedimentation method. The ratio of the exfoliated cell preservation solution in this embodiment is as follows:

[0033] Components in weight percent: 0.5% sodium chloride, O. 5% trisodium phosphate, 5% edetate disodium, edetate dipotassium, one of heparin, 1% penicillin, 1% paraformaldehyde, 4% coumarin, O.3% di Hydroxydithiothreitol, 0.1% bromo-16 alkyltrimethylamine, 5% carbolic acid, 0.5% lauryldimethylamine oxide.

[0034] Components in volume percentage: 70% pH buffer (pH7.4), 12% absolute ethanol, 10% methanol, 2% acetone, wherein the pH buffer is one of PBS, Tris-HCl, and PIPES.

[0035] The test results show that the exfoliated cell preservation solution of Example 1, Example 2 and Example 3 has high cell preservation rate, very uniform cell distribution, intact cell shape, clear boundary between cytoplasm and nucleus, distinct layers, and ...

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Abstract

The invention discloses an exfoliative cells preserving fluid which is prepared from a pH buffering agent, an osmotic pressure maintenance agent, preservatives, a fixing agent for maintaining cellular morphology, an anticoagulant, a mucus softener, an antimicrobial reagent, a cleaning agent, a humectant and red blood cell destroying components. The components in the preserving fluid are reasonable in proportioning, and the exfoliative cells can be preserved at a long time under normal temperature, wherein the longest time can reach 2 years; mucus can be sufficiently dissolved and the red cells can be partially destroyed; a film production effect is good, cellular distribution is very even, cellular morphology is perfect, cytoplasm and cell nucleus demarcation is distinct, gradation is clear, and cytoplasm and cell nucleus transparency is very good, and the exfoliative cells preserving fluid can be simultaneously used for the HPV-DNA (human papillomavirus-deoxyribonucleic acid), Chlamydia and immunohistochemical test; the special liquid base preserving fluid used for the cytologic examination of other parts can be provided, an imported product can be completely replaced, and the cellular constituent with diagnostic significance can be sufficiently preserved; and the exfoliative cells preserving fluid is low in configuration cost and easy to popularize.

Description

technical field [0001] The invention relates to an exfoliated cell preservation solution. Background technique [0002] Human living cells have physiological functions, biological activities, and inherent cell morphology and structure, and have diagnostic value in many aspects. However, if it is placed away from the original living environment, the physiological function, biological activity, and inherent cell shape and structure of the cells will change. The conventional method is to cryopreserve the exfoliated cells, and cryopreservation requires special cryopreservation. The equipment is relatively expensive, and, due to the danger of cell damage during the freezing and thawing process, the cells will mutate or deform, which will affect the follow-up work. [0003] As a screening method for cervical lesions, many factors involved in the traditional Pap smear process, such as sampling, smear quality, and cell separation, will seriously reduce the positive rate of cells. ...

Claims

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Application Information

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IPC IPC(8): A01N1/00
Inventor 刘召宏
Owner 刘召宏
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