14 results about "Cellular distribution" patented technology

The invention discloses a method and a device for detecting CAR (Chimeric Antigen Receptor) cells. The method comprises the following steps: taking a genome of a sample to be detected as a template, carrying out fluorescent quantitative PCR reaction by using a primer and a probe aiming at the sequence of a CD8 alpha transmembrane region-41 BB costimulatory molecule in a CAR gene to obtain a CT value, and calculating the copy number of the CAR gene according to the CT value and a standard curve to obtain the CAR cell distribution. Specific primers and probes are designed for CAR cells containing CD8 alpha transmembrane region-41BB costimulatory molecules, a fluorescent quantitative PCR method for detecting the copy number of CAR genes is developed, then the CAR cells are quantified, and it is proved that the method has wide applicability through multi-angle verification analysis and limitation of detection standards.

The invention relates to the technical field of stirring paddles, in particular to a uniform mixing bioreactor stirring paddle which comprises a column body, the rotating shaft is rotationally connected into the column body; through the innovative composite design of a double-layer differential transmission mechanism and vibration stirring, three-dimensional chaotic mixing is realized, and the mixing efficiency and quality are remarkably improved; the upper-layer first rotating mechanism drives a plurality of first stirring paddles to rotate at a set speed through a gear train formed by a first gear ring, a first gear and a second gear ring; the second rotating mechanism at the lower layer drives a second stirring paddle through another independent gear system, slides in a corrugated groove with a specific angle through a T-shaped block, and generates periodic radial vibration in combination with an elastic component; the rotation-vibration composite motion breaks the flow field symmetry of traditional one-way stirring, can effectively eliminate a mixing dead zone, enables nutrient substances, dissolved oxygen and cells to be highly uniformly distributed, and is especially suitable for a high-density and high-viscosity culture system.

The invention relates to the technical field of biology, in particular to a cryopreserved stem cell diluent and application thereof.The cryopreserved stem cell diluent further comprises cysteine and polyvinylpyrrolidone besides a compound electrolyte injection, the concentration of cysteine in the cryopreserved stem cell diluent is 1-10 mM, and the concentration of polyvinylpyrrolidone in the cryopreserved stem cell diluent is 1-10 mM. The concentration of the polyvinylpyrrolidone in the cryopreserved stem cell diluent is 0.02 to 0.1 g / mL. The cryopreserved stem cell diluent can improve the cell activity and cell distribution after the cryopreserved cell preparation is recovered, and can monitor the application of cells in rat living body imaging.

This invention discloses a method for analyzing the distribution of plasmablasts and Treg cells based on tissue section images, belonging to the field of plasmablast and Treg cell distribution analysis technology. The method includes the following steps: labeling plasmablasts and Treg cells in tissue section images; statistically analyzing the distribution ratio of plasmablasts and Treg cells; calculating the mixed parameters of plasmablasts and Treg cells in the tissue section images; obtaining tissue section images with different prognostic effects as sample images and extracting mixed sample parameters; analyzing the correlation between mixed parameters and prognostic effects based on the mixed sample parameters; and determining whether the prognosis of mice is good based on the distribution of plasmablasts and Treg cells and the correlation. This invention addresses the problem that existing plasmablast and Treg cell distribution analysis techniques lack comprehensive and in-depth analysis of the distribution of plasmablasts and Treg cells, leading to biased assessments of prognostic effects.

This application relates to a method, apparatus, computer equipment, and storage medium for selecting red blood cell detection regions. The method involves inputting a cell image into a pre-trained deep neural network to predict cell distribution and generate a first heatmap. Regions with more uniform cell distribution correspond to higher heatmap values. The first heatmap is then filtered using N preset filter boxes of different sizes to obtain N second heatmaps. The coordinates of the highest heatmap value in each second heatmap are extracted, and a selection box of the same size as the corresponding filter box is generated centered on the coordinates of the highest heatmap value on the cell image. Cell edges within each selection box are extracted, and the circular features of cells are detected based on these edges. Cells within the selection boxes are then screened based on these circular features, and the number of red blood cells within each selection box is counted. The selection box with the closest red blood cell count to the target count is selected as the red blood cell detection region, effectively improving the efficiency of red blood cell analysis and detection.

The invention provides an AI-based anemia risk analysis method and system, a terminal and a storage medium, and relates to the technical field of medical image analysis, and the method comprises the steps: obtaining a plurality of to-be-analyzed single erythrocyte images; inputting each single red blood cell image into a local feature extraction network to obtain local features of each single red blood cell image so as to construct a feature matrix; the feature matrix is input into a risk analysis model, the risk analysis model comprises a global feature extraction network and a prediction network, and the global feature extraction network is used for calculating the attention weight of a single red blood cell through a self-attention mechanism according to the feature matrix, performing weighted aggregation on the local features based on the attention weight, and outputting global features; and the prediction network is used for outputting an anemia prediction probability according to the global features. According to the method, the context dependency relationship of the red blood cell population can be effectively modeled, the multi-cell distribution characteristics are integrated, and the requirement of large-scale blood form image automatic anemia analysis is met.

System and method for exercising cells and / or tissue in a sealed bioreactor. System includes a cartridge having a stretching means and a means to retain cells on a scaffold mounted within the cartridge during seeding. System includes a bioreactor including a sealed enclosure, the sealed enclosure housing the scaffold and the cartridge, the bioreactor enabling uniform seeding of the scaffold with the cells, the seeding occurring within the sealed enclosure to protect from environmental contamination. The bioreactor system provides a means to supply fresh media to the cells and / or tissue, and a means to remove waste products.

This invention relates to the field of image analysis technology, specifically to a method for assessing the distribution of cardiac mesothelial cells based on immunofluorescence images. The method includes: acquiring multiple immunofluorescence images of cardiac mesothelial cells at different antibody concentrations within the assessment group; determining initial cardiac mesothelial cell regions based on the staining status of the isotype control group and the differences in staining depth among multiple staining regions in the immunofluorescence images; analyzing non-specific staining based on changes in the staining area and irregular cell boundaries of the initial cardiac mesothelial cell regions, and adjusting the antibody concentration accordingly; determining multiple target cardiac mesothelial cell regions, and obtaining the location information of the cardiac mesothelial cells based on the degree of overlap and similarity between these regions; and analyzing the spacing distribution of cardiac mesothelial cells and their distribution in the immunofluorescence images based on the location information to obtain the uniformity of the cardiac mesothelial cell distribution.

The present application relates to the technical field of medical equipment monitoring, and particularly relates to a wireless temperature and humidity monitoring system and method for medical refrigerators. The system of the present application comprises a distributed wireless sensing module, an edge data processing module, a cloud monitoring platform, an intelligent early warning module and a blockchain storage module. The distributed wireless sensing terminal adopts a cellular distribution mode, integrates a high-precision temperature and humidity sensor, a laser scattering compensation component and an RFID-WiFi dual-mode communication unit, realizes global accurate collection and reliable transmission; the edge data processing module generates a three-dimensional temperature and humidity distribution model through space-time calibration and temperature field reconstruction, and predicts potential equipment failures; the intelligent early warning module performs hierarchical early warning and links the standby refrigeration unit; the blockchain storage module stores all data on the chain to ensure that the data cannot be tampered with. The present application is suitable for intelligent temperature and humidity monitoring and compliance control in medical material storage scenes such as vaccines, blood products and biological reagents.

This invention discloses a benzo[selenazole] derivative, its preparation method, and its application in the preparation of RNA fluorescent probes. The benzo[selenazole] derivative provided by this invention has the following structural formula: [structural formula would be inserted here]. This benzo[selenazole] derivative is a typical fluorescently activated probe, exhibiting excellent selective recognition and binding ability for RNA molecules. It demonstrates excellent resistance to biological background interference and exhibits high binding selectivity and recognition specificity for RNA in both live and fixed cell systems. It possesses advantages such as high signal-to-noise ratio and excellent fluorescence photostability, making it suitable for real-time, dynamic fluorescence imaging detection of intracellular RNA. It can achieve in-situ visualization and tracing of changes in subcellular RNA distribution and content. The synthesis method of the benzo[selenazole] derivative provided by this invention has a clear process flow, mild and stable reaction conditions, requires no demanding equipment or special reagents, and uses commercially available and readily available conventional chemicals as raw materials. The operation is simple and easy to perform, with strong overall process operability, making it suitable for large-scale production applications.

The invention is to provide a method of profiling a sample comprising a plurality of cells, the method comprising: flowing cells from the sample through a first array of pillars to obtain one or more distribution profiles of cells sorted by the first array; flowing cells from the sample through a second array of pillars that is different from the first array of pillars to obtain on one or more distribution profiles of cells sorted by the second array; and deriving a biophysical signature of the sample based on at least the one or more distribution profiles of the cells sorted by the first array and / or the one or more distribution profiles of the cells sorted by the second array. The method further comprises determining a health status of a subject based on the biophysical signature of the sample. The invention is also to provide a sample profiling system. In various embodiments, the distribution profile of cells in the output regions is indicative of one or more biophysical properties of the cells, which may include the size and deformability of the cells. The pillars in the first array and the second array may have a shape selected from the group consisting of a substantially L shape and a substantially inverse L shape, mirror reflections thereof or combinations thereof.

The invention belongs to the technical field of digital pathological image analysis, and particularly relates to a cell distribution three-dimensional reconstruction method of a planar HE staining pathological image, which comprises the following steps: acquiring a single two-dimensional HE staining pathological image under a 40-time visual field of an optical microscope, and preprocessing the single two-dimensional HE staining pathological image to obtain preprocessed data; acquiring cell characteristics of each cell nucleus in the preprocessed data; constructing a cell Z-axis distribution probability model by adopting an EM algorithm, and generating an initial Z-axis coordinate of each cell nucleus in the preprocessed data by adopting a Z-axis distribution sampling method; aiming at each cell nucleus in the preprocessed data, carrying out cell initialization according to cell characteristics and initial Z-axis coordinates; executing a PSO-EM hybrid algorithm based on the initialized cells, and generating a three-dimensional center coordinate and a radius of each cell; according to the method, the problem of cell space information loss of the two-dimensional plane pathological image is solved.

The invention discloses a cleavage-stage embryo medical image quality detection method and system, and relates to the technical field of medical image detection.The cleavage-stage embryo medical image quality detection method comprises the following steps that S1, a cleavage-stage embryo is placed in a matched agarose culture dish, and the refractive index of the agarose culture dish is consistent with that of an embryo culture solution; s2, constructing a 4D imaging model, performing continuous dynamic imaging on the cleavage stage embryo, and collecting 4D original image data of the cleavage stage embryo within a preset time range; s3, constructing a preprocessing model, carrying out refraction correction on the 4D original image data, identifying an embryo contour and cell distribution, judging an embryo body position, and forming preprocessed image data; the agarose culture dish with the refractive index consistent with that of the embryo culture solution is adopted and matched with the exclusive refraction correction sub-module for light refraction deviation compensation, refraction deviation is eliminated from the source and post-processing two dimensions, the problems of image blurring and distortion are effectively solved, and the basic quality of images is improved.

A composition comprising edible hydrogel microcarriers-comprising cells, wherein said cells are distributed throughout said hydrogel microcarriers such that said hydrogel microcarriers are devoid of a cell-free core. Also provided are methods or producing and using same.