5 results about "Cellular distribution" patented technology

This invention discloses a method for analyzing the distribution of plasmablasts and Treg cells based on tissue section images, belonging to the field of plasmablast and Treg cell distribution analysis technology. The method includes the following steps: labeling plasmablasts and Treg cells in tissue section images; statistically analyzing the distribution ratio of plasmablasts and Treg cells; calculating the mixed parameters of plasmablasts and Treg cells in the tissue section images; obtaining tissue section images with different prognostic effects as sample images and extracting mixed sample parameters; analyzing the correlation between mixed parameters and prognostic effects based on the mixed sample parameters; and determining whether the prognosis of mice is good based on the distribution of plasmablasts and Treg cells and the correlation. This invention addresses the problem that existing plasmablast and Treg cell distribution analysis techniques lack comprehensive and in-depth analysis of the distribution of plasmablasts and Treg cells, leading to biased assessments of prognostic effects.

This invention relates to the field of image analysis technology, specifically to a method for assessing the distribution of cardiac mesothelial cells based on immunofluorescence images. The method includes: acquiring multiple immunofluorescence images of cardiac mesothelial cells at different antibody concentrations within the assessment group; determining initial cardiac mesothelial cell regions based on the staining status of the isotype control group and the differences in staining depth among multiple staining regions in the immunofluorescence images; analyzing non-specific staining based on changes in the staining area and irregular cell boundaries of the initial cardiac mesothelial cell regions, and adjusting the antibody concentration accordingly; determining multiple target cardiac mesothelial cell regions, and obtaining the location information of the cardiac mesothelial cells based on the degree of overlap and similarity between these regions; and analyzing the spacing distribution of cardiac mesothelial cells and their distribution in the immunofluorescence images based on the location information to obtain the uniformity of the cardiac mesothelial cell distribution.

The present application relates to the technical field of medical equipment monitoring, and particularly relates to a wireless temperature and humidity monitoring system and method for medical refrigerators. The system of the present application comprises a distributed wireless sensing module, an edge data processing module, a cloud monitoring platform, an intelligent early warning module and a blockchain storage module. The distributed wireless sensing terminal adopts a cellular distribution mode, integrates a high-precision temperature and humidity sensor, a laser scattering compensation component and an RFID-WiFi dual-mode communication unit, realizes global accurate collection and reliable transmission; the edge data processing module generates a three-dimensional temperature and humidity distribution model through space-time calibration and temperature field reconstruction, and predicts potential equipment failures; the intelligent early warning module performs hierarchical early warning and links the standby refrigeration unit; the blockchain storage module stores all data on the chain to ensure that the data cannot be tampered with. The present application is suitable for intelligent temperature and humidity monitoring and compliance control in medical material storage scenes such as vaccines, blood products and biological reagents.

This invention discloses a benzo[selenazole] derivative, its preparation method, and its application in the preparation of RNA fluorescent probes. The benzo[selenazole] derivative provided by this invention has the following structural formula: [structural formula would be inserted here]. This benzo[selenazole] derivative is a typical fluorescently activated probe, exhibiting excellent selective recognition and binding ability for RNA molecules. It demonstrates excellent resistance to biological background interference and exhibits high binding selectivity and recognition specificity for RNA in both live and fixed cell systems. It possesses advantages such as high signal-to-noise ratio and excellent fluorescence photostability, making it suitable for real-time, dynamic fluorescence imaging detection of intracellular RNA. It can achieve in-situ visualization and tracing of changes in subcellular RNA distribution and content. The synthesis method of the benzo[selenazole] derivative provided by this invention has a clear process flow, mild and stable reaction conditions, requires no demanding equipment or special reagents, and uses commercially available and readily available conventional chemicals as raw materials. The operation is simple and easy to perform, with strong overall process operability, making it suitable for large-scale production applications.

The invention is to provide a method of profiling a sample comprising a plurality of cells, the method comprising: flowing cells from the sample through a first array of pillars to obtain one or more distribution profiles of cells sorted by the first array; flowing cells from the sample through a second array of pillars that is different from the first array of pillars to obtain on one or more distribution profiles of cells sorted by the second array; and deriving a biophysical signature of the sample based on at least the one or more distribution profiles of the cells sorted by the first array and / or the one or more distribution profiles of the cells sorted by the second array. The method further comprises determining a health status of a subject based on the biophysical signature of the sample. The invention is also to provide a sample profiling system. In various embodiments, the distribution profile of cells in the output regions is indicative of one or more biophysical properties of the cells, which may include the size and deformability of the cells. The pillars in the first array and the second array may have a shape selected from the group consisting of a substantially L shape and a substantially inverse L shape, mirror reflections thereof or combinations thereof.