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Tissue engineering vessel and preparation method and application thereof

A technology of tissue engineering and blood vessels, applied in the field of tissue engineering blood vessels and its preparation, can solve the problems of uneven distribution of extravascular cells, unsuitability for cell migration and proliferation, and poor performance, and achieve rich collagen fibers, moderate hardness, and excellent The effect of the characteristic

Inactive Publication Date: 2013-08-07
SHANGHAI CHILDRENS MEDICAL CENT AFFILIATED TO SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

In order to simulate living blood vessels, the wall thickness of the directly electrospun tubular scaffold material is basically the same as that of the living blood vessels, which is generally thicker, which is not suitable for cell migration and proliferation, and because the cells are directly planted on the outer surface of the scaffold material, resulting in the construction of Inhomogeneous distribution of cells inside and outside tissue engineered blood vessels and poor performance

Method used

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  • Tissue engineering vessel and preparation method and application thereof
  • Tissue engineering vessel and preparation method and application thereof
  • Tissue engineering vessel and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Example 1 Preparation of tissue engineered blood vessels of the present invention (1)

[0032] 1 Materials and methods

[0033] 1.1 Preparation of gelatin / polycaprolactone electrospun membrane

[0034] Weigh 0.5g of gelatin and 0.5g of polycaprolactone with an electronic analytical balance, dissolve them in 10ml of trifluoroethanol, stir for 24 hours until completely dissolved, and obtain gelatin / polycaprolactone with a concentration of 10% (g / ml) for spinning solution, add 10 μl acetic acid to the solution, and stir for 10-20 min until the solution turns from cloudy to clear. Select a 10ml syringe and a needle with an inner diameter of 1.2mm to extract the gelatin / polycaprolactone spinning solution, fix it on the electrospinning device, adjust the electrostatic voltage to 10kv, the acceptance distance to 12cm, and the injection rate to 2ml / h. Spinning, using aluminum foil as a receiving device, spinning for 3 hours to obtain a gelatin / polycaprolactone composite nan...

Embodiment 2

[0054] Example 2 Preparation of tissue engineered blood vessels of the present invention (2)

[0055] The preparation method is the same as in Example 1, except that the prepared rectangular gelatin / polycaprolactone electrospun membrane has a width of 2 cm and a length of 13 cm; The urinary catheter is curled into a tubular shape by the "egg roll" method, and the number of coils is: 13cm / (6×3.14)=6.9 circles. Results: The prepared tissue-engineered blood vessels were the same as those in Example 1, with clear borders, good elasticity, moderate hardness, smooth inner walls, and independent support into tubes when placed flat; HE sections showed dense tissue structures and uniform cell distribution, Masson Staining showed that collagen fibers were abundant in the constructed tissue engineering blood vessels.

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Abstract

The invention relates to a tissue engineering vessel and a preparation method and an application thereof. The tissue engineering vessel uses a tubular gelatin / polycaprolactone electricity texture membrane as a support, and smooth muscle cells are evenly distributed in the support. The preparation method includes: a) spreading disinfected gelatin / polycaprolactone materials in a culture dish; b) collecting umbilical artery smooth muscle cells, evenly inoculating the umbilical artery smooth muscle cells on the surface of the gelatin / polycaprolactone electricity texture membrane to culture over the night, and forming a cell material composite electricity texture membrane; and c) twisting the cell material composite electricity texture membrane on a polyvinyl chloride (PVC) tube for culturing, and finally forming the tubular tissue engineering vessel. Cells of the tissue engineering vessel prepared by the method are evenly distributed, simultaneously the tissue engineering vessel has the advantages of being good in elasticity, moderate in hardness and compact in tissue structure, having smooth inner walls and abundant collagenous fibers, and being clear in self tissue boundary and the like. The characteristics of the tissue engineering vessel are close to that of living body vessels.

Description

technical field [0001] The invention relates to the technical field of tissue engineering, in particular to a tissue engineering blood vessel and its preparation method and application. Background technique [0002] Vascular disease is one of the diseases with the highest mortality rate in my country at present, and vascular transplantation is its main surgical treatment. In vascular transplantation, it is necessary to use tissue engineered blood vessels to replace diseased blood vessels. Tissue engineered blood vessels use tissue engineering methods to compound blood vessel seed cells (endothelial cells, smooth muscle cells) with related extracellular matrix (scaffold materials) to construct tissue engineered blood vessels that are close to living blood vessels in terms of shape and function. Tissue-engineered blood vessels with excellent performance should have the advantages of clear boundaries with their own tissues, certain elasticity and hardness, dense tissue structu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61F2/06
Inventor 付炜冯蓓殷猛王伟
Owner SHANGHAI CHILDRENS MEDICAL CENT AFFILIATED TO SHANGHAI JIAOTONG UNIV SCHOOL OF MEDICINE
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