38 results about "Acinus" patented technology

The invention discloses a multiple stain reagent and a detection method for identifying a breast myoepithelial lesion. The multiple stain reagent comprises 20% of mouse anti-human CK8/18 immunohistochemical monoclonal antibody, 20% of ready-to-use calmodulin mouse anti-human monoclonal antibody, 20% of ready-to-use cytokeratin mouse anti-human monoclonal antibody, 20% of ready-to-use P63 protein mouse anti-human monoclonal antibody, and 20% of actin HHF-35 anti-human monoclonal antibody. When a breast myoepithelial cell is marked, the myoepithelial cell has strong specificity and sensibility, and different target antigens in the same target cell can be identified, so that a cell nucleus, a cell membrane and a cytoplasm can be stained synchronously, the reactivity and the sensibility of the myoepithelial cell are markedly improved, basic structures of a breast acinus and a catheter can be indicated more objectively, and a good assistance effect is exerted on the diagnosis of the breast lesion.

The invention provides application of novel glucagon peptide 1 fatty acid modified or unmodified dimer different in configuration in pancreas protection or hypoglycemic effect during treatment of II-type diabetes. The dimer is formed by connecting two identical GLP-1 monomers containing cysteine through disulfide bond formed by cysteine oxidation. Hypoglycemic duration of GLP-1 dimer is remarkablyprolonged without lowering activity of H-shaped GLP-1 homodimer (the disulfide bond is formed inside peptide chain), in-vivo continuous activity of GLP-1 analogue dimer can last for 19d while in-vivoactivity of liraglutide which is positive control drug is 3d, or in-vivo activity duration is remarkably prolonged when compared with long-acting GLP1 similar peptide which has already been reportedat present, so that technical progress of long-acting GLP1 drug is greatly promoted while convenience is brought to clinical application and popularization of the same. U-shaped homodimer (the disulfide bond is formed at the terminal C of the peptide chain) does not have impact on blood glucose but can obviously protect exocrine portion cells like pancreatic acini and catheters, thereby having a pancreas protecting function.

To provide a cell culture method capable of sustaining in-vivo functions for a long time by suppressing dedifferentiation. A cell culture method in accordance with the present invention is to culture cells in a multi-layered state in a minute partitioned space and to obtain a tissue structure having a function resembling an in-vivo function. When the cells are mammary gland epithelial cells, a hollow acinus-like structure can be formed. The minute partitioned space is particularly preferably a micro container in a cell culture container having a plurality of such micro containers on the surface.

The invention discloses a method for building a dairy cattle breast acinus lactation model in vitro, and relates to a method for building a lactation model in vitro. The method solves the problem of low authenticity of in-vivo environment caused by taking a mammary epithelial cell as a research carrier in the existing in-vitro study of the lactation function of the dairy cattle breast. The method comprises the following steps: 1, preparing a tissue culture plate of matrix gel; 2, digesting primary cultured dairy cattle mammary epithelial cell gestated for 4 months by adopting pancreatin and EDTA, and collecting cells after centrifugalization; 3, preparing cell suspension and diluting; and 4, adding the cell suspension into the tissue culture plate of the matrix gel, culturing for 15 days in an incubator to finish the building. The artificial building process of the dairy cattle breast acinus lactation model built by the invention is a simulation process of in-vitro simulation of a galactemia protective screen; and a three-dimensional acinus structure thereof can truly simulate in-vivo situation, has the same lactation function and biological characteristics as the breast acinus in a ruminant, and has high authenticity of in-vivo environment simulation.

The invention discloses application of berberine or other pharmaceutically acceptable salts in preparing a M3 receptor selective inhibitor medicine. The medicine is used for treating acute pancreatitis, lung cancers and colon cancers, self-immunity sialadenitis, asthma, respiratory tract inflammation, lung diseases and cardiovascular diseases, relieving panic, or treating excessive activity of bladders, and is used for inhibiting the enhancing of Ca<2+> signals in pancreatic acinar cells caused by M3 receptor-mediated acetyl choline and L-arginine.

The invention discloses a composition with a Staphylococcus aureus resisting effect. The composition consists of general flavone of sophora flavescens and general flavone of liquorice. The general flavone parts of sophora flavescens and liquorice are prepared through extraction, extraction, enrichment and the like, and are compounded. The results of in-vitro research shows that the composition directly inhibits the growth of gram-positive bacteria such as Staphylococcus aureus and can reduce the formation of biofilms and the content of staphyloxanthin; the results of in-vivo studies prove thatthe composition can obviously reduce mammary gland alveolar hyperplasia and alveolar inflammatory cell infiltration of mice caused by Staphylococcus aureus infections, and significantly reduce the bacterium carrying amount of mouse mammary gland tissues of each model group and the content of IL-1 beta, IL-2 and TNF-alpha inflammatory factors in the mammary gland tissues. According to the invention, the comprehensive utilization of medicinal materials including sophora flavescens and liquorice and the quality and efficiency improvement of the industry can be realized; the waste is turned intothe wealth; and the composition has good economic effects and social benefits, and accords with the sustainable development strategy in China.

The invention discloses application of formononetin in preparation of a medicine for preventing and treating acute pancreatitis diseases, and belongs to the field of biological medicines. According to the invention, by utilizing the formononetin, pancreatic edema is relieved, the content of serum amylase and serum lipase is reduced, acinus cell injury and inflammation infiltration are relieved, colon goblet cell injury and inflammation infiltration caused by acute pancreatitis are relieved and release of proinflammatory cytokines in pancreas and colon is reduced. According to the invention, the formononetin is used for preparing the medicine and the medicine composition for treating the acute pancreatitis and the related diseases of the acute pancreatitis, and the application field of the formononetin is widened.

The invention discloses an application of flavonoids in coreopsis tinctoria and pharmaceutical compositions thereof in preparing medicines for treating acute pancreatitis, and provides an applicationof flavonoids in preparing medicines for treating acute pancreatitis. The flavonoids can significantly alleviate the necrosis of pancreatic acinar cells and protect the local and systemic symptoms oftwo types of acute pancreatitis. The application will fill up the medicinal value of the coreopsis tinctoria and the flavonoids obtained therefrom through separation in pancreatitis protection, provides new diagnosis and treatment modes and application prospects for clinics and provides a new choice for clinically screening and/or preparing the medicines for treating acute pancreatitis.

The invention discloses miRNA for preventing and/or treating acute pancreatitis, and pharmaceutical application of the MiRNA, and belongs to the technical field of biological medicines. The invention provides application of a miR-133a agonist in preparation of a medicine for inhibiting the acute pancreatitis, and makes clear that the miR-133a agonist is miRNA of which the nucleotide sequence is shown as SEQ ID NO: 1. Through simulation of a clinically related acute pancreatitis model, the miR-133a agonist is proved to be capable of remarkably improving occurrence and development of the above AP model, and can be used for clinically preventing/ treating the AP. Meanwhile, the invention also proves through an in-vitro acinus cell AP model that the protection effect of the miR-133a agonist for the acute pancreatitis is that macrophages carry out targeted "removal" on the damaged acinus cells to realize the functions of alleviating the AP inflammatory response and reducing AP severity. The invention provides a new drug source for prevention, diagnosis, detection, protection, treatment and research of pancreatitis diseases, can be easily popularized and applied clinically, and can generate a huge clinical application prospect and social benefit in a relatively short time.

The invention discloses a traditional Chinese medicine ointment for treating mastitis. The traditional Chinese medicine ointment comprises the following raw materials in parts by weight: 4g of menthol, 50g of herba violae, 50g of herba taraxaci and 2g of borneol. According to the application and the method of the traditional Chinese medicine ointment for treating the mastitis, a damage condition of mammary tissue is evaluated by mammary tissue paraffin sectioning and H-E staining methods, and the activity of MPO (myeloperoxidase) of mammary tissue and the expression level of inflammatory factors in the mammary tissue are detected, which proves that the traditional Chinese medicine ointment can relieve damage to the mammary tissue of a mouse; inhibits mammary gland tissue inflammatory cell infiltration and inflammatory factor expression and relieves the atrophy of mammary gland acinus, thereby effectively showing that the traditional Chinese medicine ointment can relieve the inflammatory reaction of mammary gland and has a good treatment effect on LPS-induced mouse mastitis.

The invention discloses application of Bifidobacteria pseudococcus tenophorae, Bifidobacteria or Bifidobacteria animations in the aspect of preventing or treating acute pancreatitis, and particularly discloses application of Bifidobacteria pseudococcus tenophorae, Bifidobacteria animations or Bifidobacteria animations in the aspect of preventing or treating acute pancreatitis. The invention provides an application of Bifidobacterium pseudocausate, Bifidobacterium adolescence or Bifidobacterium aniis in a product for preventing or treating acute pancreatitis, and the application of the Bifidobacterium pseudocausate, the Bifidobacterium adolescence or the Bifidobacterium aniis in a product for preventing or treating acute pancreatitis. The invention further provides a preparation method of the Bifidobacterium pseudocausate, the Bifidobacterium adolescence and the Bifidobacterium aniis. The strain can relieve alveolar cell injury and inflammation infiltration, reduce the concentration of amylase in serum and reduce the death rate of acute pancreatitis, is used for preparing probiotic medicines, pharmaceutical compositions, health-care foods, functional foods and feeds for treating acute pancreatitis, and has a wide application prospect.

The invention provides a construction method and application of an acute pancreatitis cell model, and belongs to the field of cell models. It is found for the first time that glucose treatment can significantly increase the number of pancreatic acinus necrosis cells and significantly reduce the half-maximum effect time, and an acute pancreatitis cell model is successfully constructed. It is found for the first time that glucose combined fatty acid treatment can further increase and induce the pancreatic acinus cell necrosis degree, and an acute pancreatitis cell model is successfully constructed. The adopted raw materials are easy to obtain and low in price, and the modeling cost is reduced; according to the invention, the acute pancreatitis cell model can be successfully constructed in a short time, and the modeling time is shortened. The modeling method is convenient and fast, easy to operate, low in cost and good in repeatability. The acute pancreatitis cell model constructed by the invention not only can be used for screening acute pancreatitis treatment drugs, but also can provide a good platform for the occurrence and development mechanism of acute pancreatitis, and has a wide application prospect.

The invention discloses application of Akkermansia muciniphila in the aspect of treating chronic pancreatitis, and belongs to the application field of probiotics. The invention provides the application of an Akkermansia muciniphila BAA-835 strain in preparation of a product for treating chronic pancreatitis, and the strain can relieve pancreatic atrophy symptoms, acinus cell injury and inflammation infiltration, reduce release of proinflammatory cytokines and infiltration of macrophages, relieve pancreatic fibrosis degree in an active state or an inactive state, improve pancreatic fibrosis resistance and improve pancreatic fibrosis resistance. The compound can be used for preparing medicines, pharmaceutical compositions, functional foods and feeds for treating chronic pancreatitis, and has a wide application prospect.

The invention discloses a novel electro-spinning nano-stent available for tracheal epithelial implantation of a rat and a preparation method of the novel nano-stent, wherein the component materials comprise lactic acid-glycollic acid copolymer and silk fibroin in a mass ratio of (1-3):(7-9); and lactic acid-glycollic acid copolymer and silk fibroin are mixed to prepare a nanoscale reticular fiber plane stent. The novel nano-stent available for epithelial implantation provided by the invention is beneficial to the adhesion and proliferation of cells, can be used for repairing tracheal epithelium, alimentary canal epithelium, digestive gland acinus, catheter and the like, can be particularly used for repairing trachea defects, and provides meaningful references for promoting the repairing of tracheal mucosa and the clinical reconstruction of trachea defects, and reducing postoperative complications of trachea reconstruction.

The invention provides a simulated major salivary gland model device and a preparation method thereof based on the salivary gland image data of real people. The device is used for training a salivary gland endoscopic operation, and the mastery of operation skills by a doctor is facilitated. The device comprises a major salivary gland model constructed based on the salivary gland image data of the real people and a plurality of pathological models constructed based on common clinical diseases. The major salivary gland model comprises a three-level catheter system with a dendritic structure and acini which are connected to the end of the catheter system. The ends of the third-level catheters of the catheter system extend into the acinus. The third-level catheters converge to second-level catheters which converge to a first-level catheter. Each of the pathological models is provided with a main catheter channel which is matched with the first-level catheter of the major salivary gland model and can be connected to the major salivary gland model in a replaced way respectively. When two models are connected to be a whole body, the main catheter channels of the pathological models are connected to the first-level catheters of the major salivary gland models.

The invention relates to the technical field of traditional Chinese medicine preparations, in particular to a medicine, Yutusu, for treating hyperplasia of mammary glands (known as lump in breast in traditional Chinese medicine) and a preparation method and application thereof. The medicine is prepared from an ethanol extract of Angelica keiskei, a water extract of malt and a water extract of hawthorn. By comparing the treatment of model rats with hyperplasia of mammary glands, the compound medicine "Yutusu" is found to be effective in: (1), reducing the serum estradiol content and increasingthe level of progesterone hormone in serum; (2), reducing the number of lobular hyperplasia in breast tissue; and (3), reducing the number of occlusive obstructed acini in the lobules of the breast tissue; and the medicine has a good effect of soothing the liver and eliminating lump in breast.