63 results about "Sodium butyrate" patented technology

The invention discloses a milk-flavor jelly-shaped intelligent response type suckling pig creep compound feed and a preparation method, relates to the technical field of animal feeds, and aims to solve the problems that a traditional feed is poor in palatability, nutrition release and physiological needs are mismatched, thermosensitive components are damaged by a high-temperature process and the like. And accurate release of nutrients is realized. The feed comprises an energy raw material, a protein raw material, intelligent response components (pH-sensitive sodium alginate microsphere loaded sodium butyrate / glutamine, temperature-sensitive xanthan gum-locust bean gum compound gel coated antibacterial peptide, and enzyme-sensitive lysine-lysine peptide bond cross-linked layer embedded glucose compound enzyme), a flavor system (vanillin microcapsules) and a konjac gum jelly matrix. A pH-temperature-enzyme triple response mechanism is adopted to adapt to the gastric acid gradient, body temperature fluctuation and pancreatin activity of suckling pigs; milk flavor slow release and nutrition release are in space-time synergy, and the ingestion frequency is increased by 40%; the konjac glucomannan matrix reduces dust, and the gel strength is greater than or equal to 750g / cm.

The invention discloses application of sodium butyrate in preparation of a medicine for relieving brain injury caused by gas explosion, the sodium butyrate regulates nerve cell ferroptosis through a JNK / P38 MAPK pathway to relieve the brain injury caused by gas explosion, firstly, an animal model of rat brain injury caused by gas explosion is established, and the relation between NaB and brain tissue neuron injury is intervened and observed by applying NaB; secondly, a shock wave physiotherapy instrument is used for impacting a co-culture system of three cells of CTX, H19-7 and GMI-R to simulate explosion to construct an in-vitro experimental model, P38, ERK and Fer-1 inhibitors are adopted for intervention, iron metabolism, ferroptosis and MAPK signal channel factor changes are observed from the cell and molecular level, a mechanism for inhibiting ferroptosis through a related cascade signal channel mediated by NaB-mediated intestinal-brain axis regulation and control is clarified, and the effect of inhibiting ferroptosis is achieved. And a theoretical basis is provided for further explaining the action mechanism of the gas explosion brain injury.

The invention discloses application of sodium butyrate to prevention and treatment of dairy cow mastitis, and belongs to the technical field of biology. The invention can provide an application of sodium butyrate in preventing and treating cow mastitis, in particular an application in preparing a preparation for preventing and treating cow mastitis induced by lipopolysaccharide (LPS) in escherichia coli. According to the application of the sodium butyrate in the aspect of preventing and treating the dairy cow mastitis, expression of key factors such as mammary gland tight junction protein is up-regulated under stimulation of lipopolysaccharide (LPS), and the sodium butyrate can effectively inhibit apoptosis and enhance the barrier function of mammary gland acinus, so that the effect of preventing and treating the dairy cow mastitis is achieved.

The invention discloses a culture method for enhancing the body color of Dongmaotai, and relates to the technical field of aquaculture. The method comprises the following steps: (1) illumination induction: alternately irradiating by adopting a light source white-red-blue-yellow, (2) feeding with a toner: feeding with basal feed at the same time, and (3) water flow stimulation: regulating physiological metabolism, nutrition absorption and environmental adaptability to realize the purpose of improving the quality of the feed by regulating the physiological metabolism, nutrition absorption and environmental adaptability. The method is beneficial to enhancing the body color of the east astroplatia and improving the economic value of the east astroplatia.

The invention discloses a porcine beta-defensin-3 gene core promoter as well as a construction method and application thereof, and belongs to the technical field of gene engineering. The sequence of the core promoter is as shown in SEQ ID No.1, and the core promoter has remarkable promoter activity in porcine small intestine epithelial cells and can be regulated and controlled by nutrient substances sodium butyrate and glutamine. The invention also discloses a construction method for specifically amplifying the core promoter, which comprises the following steps: carrying out PCR (Polymerase Chain Reaction) amplification by taking porcine small intestine epithelial cell DNA (Deoxyribonucleic Acid) as a template, constructing a pMD-18T-pBD-3-P recombinant plasmid by taking upstream and downstream primers as shown in SEQ No.2-SEQ No.3, and amplifying by taking the recombinant plasmid as a template to obtain a core promoter fragment. The core promoter disclosed by the invention provides an excellent experimental system for researching a transcription regulation mechanism of the porcine beta-defensin-3 gene, the nutritional response characteristic of the core promoter provides a new platform for researching nutrition-immune interaction, and the core promoter has a wide application prospect in the field of healthy breeding of livestock and poultry.

The application discloses a suspension for improving DON-caused piglet intestinal toxicity and a preparation method and application thereof, and belongs to the technical field of feed additives, and comprises the following raw materials: hesperidin 40-60 mg / mL, sodium butyrate 50-150 mg / mL and PR-39 antibacterial peptide 10-15 mg / mL. The suspension provided by the application can also regulate intestinal flora balance, promote the proliferation of beneficial bacteria such as lactobacillus, and inhibit the growth of harmful bacteria. The effect of the suspension provided by the application is significantly better than that of single component or partial combination, and the suspension provides an efficient and safe nutritional intervention strategy for preventing and treating animal body damage caused by feed mycotoxin pollution.

The invention provides a high-density suspension transfection method for efficiently obtaining a lentiviral vector. The method comprises the following steps: accurately regulating and controlling the density of HEK293T cells to 5.5-6.5 * 10 < 6 > cells / ml, mixing GOI, pL-Gagpol, pL-Rev and pL-VSVG plasmids according to a molar ratio of 4: 2: 1: 0.6, and forming a transfection compound according to a ratio of PEI to total plasmids of 2.5: 1; and after transfecting for 24 hours, adding 5 mM of sodium butyrate, and continuously culturing for 72 hours to obtain the virus. According to the method, the traditional density limitation is broken through, the transduction titer is increased by 3 times when the density is 6 * 10 < 6 > cells / ml, special equipment is not needed, and the production cost is greatly reduced. The produced lentiviral vector is suitable for the field of gene therapy such as CAR-T cell therapy, the titer of the lentiviral vector is larger than or equal to 2.5 * 10 < 7 > TU / ml, and refractory target cells can be efficiently transduced.

Provided are pharmaceutical compositions comprising a combination therapy having therapeutically effective amounts of sodium 4-phenyIbutylate and tauroursodeoxycholic acid for the treatment of an ocular condition in a subject. Also provided herein are methods using the combination therapy, and a pharmaceutical composition thereof, for preventing or treating an ocular condition in a subject.

The invention discloses a nano antibacterial dressing and a preparation method thereof, and belongs to the technical field of pharmaceutical preparations. According to the nano-antibacterial dressing, mupirocin serves as an antibacterial component, chitosan, zein, tragacanth, sodium hyaluronate, sodium butyrate, a cross-linking agent, a humectant and other components are supplemented, and the nano-antibacterial dressing has efficient antibacterial performance, outstanding stability and simple preparation process and has important application value in wound healing and infection prevention and control.

A lentiviral vector production system comprises (a) a lentiviral culture supplement to control cell growth, (b) a transfection reagent comprising DHDMS, DOPE, and cholesterol to increase transfection efficiency, (c) a lentiviral production enhancer comprising sodium propionate, sodium butyrate, and caffeine to boost lentiviral production, wherein the lentiviral vector production system is serum-free. A method of lentiviral vector production comprises using the lentiviral production system. Another method for lentiviral vector production comprises (a) culturing eukaryotic cells in a serum-free medium, (b) providing a lentiviral culture supplement to control cell growth, (c) transfecting the cells with a lentiviral vector using a transfection reagent comprising DHDMS, DOPE, and cholesterol to increase transfection efficiency, and (d) providing a lentiviral production using a lentiviral production enhancer comprising sodium propionate, sodium butyrate capable of boosting lentiviral production.

The application discloses application of sodium butyrate in preparation of a medicine for preventing and treating grass carp hemorrhagic disease, wherein a structural formula of the sodium butyate is molecular formula C4H7NaO2, and a CAS number is 156-54-7; and the application further provides a medicine composition which comprises an effective dose of the sodium butyrate as an active ingredient, and further comprises a pharmaceutically acceptable carrier or excipient, and the dosage form comprises tablets, granules, powders, powders, suspensions, oral liquids, and the concentration of the sodium butyrate is 0.1-20 mM. Through a cell experiment, it is found that the sodium butyrate can significantly inhibit replication of grass carp reovirus in grass carp kidney cells, and improve expression of immune genes of the grass carp kidney cells, and it is proved that the sodium butyrate has a high efficient inhibiting effect on infection of the grass carp reovirus, and has the advantages of high safety and wide application prospect for preparing the medicine for preventing and treating the grass carp hemorrhagic disease caused by the grass carp reovirus.

The application belongs to the field of feed additives, and particularly discloses application of sodium butyrate in preparation of a feed additive against chicken white dysentery Salmonella, and sodium butyrate is added in chicken basic feed as the feed additive against chicken white dysentery Salmonella; the addition of sodium butyrate in chicken basic feed can significantly reduce the bacterial load of chicken white dysentery Salmonella infection in chicken liver and spleen, significantly increase the ratio of villus length to crypt depth of the cecum of chicken infected with chicken white dysentery Salmonella, and improve the intestinal absorption capacity; the content of CD3+ T cells, TCR+ T cells, macrophages and B cells in the liver of chicken infected with chicken white dysentery Salmonella can be significantly reduced; and then the body weight of chicken infected with chicken white dysentery Salmonella is significantly increased, and the survival rate of chicken infected with chicken white dysentery Salmonella is increased. In summary, the addition of sodium butyrate in chicken basic feed can enhance the resistance to chicken white dysentery Salmonella infection.

The application belongs to the technical field of feed additives, discloses a compound functional additive for relieving liver oxidative stress and endoplasmic reticulum stress of mandarin fish, and specifically discloses a feed additive which comprises the following components: sodium octanoate, sodium butyrate and rice husk powder. The additive provided by the application can effectively relieve liver oxidative stress and endoplasmic reticulum stress of mandarin fish, reduce the content of malondialdehyde, improve the ability of mandarin fish to resist oxidative enzyme activity, and is beneficial to the health of fish body.

The invention relates to the technical field of medicines, in particular to combined application of semeglutide and sodium butyrate in preparation of a medicine for reducing weight. Experimental results show that when the semeglutide and the sodium butyrate are combined for use, the appetite inhibition effect can be synergistically enhanced, and the body weight and the fat weight of a mouse are remarkably reduced.

The application discloses a kind of low morbidity feed for California bass antibiotic-free breeding and preparation method thereof, belong to aquatic feed field.The feed is composed of 60~80 parts of basic feed component, 10~20 parts of fermented plant protein, 2~6 parts of functional compound additives.Fermented plant protein is prepared by compound fermentation of butyric acid clostridium and bacillus subtilis from soybean meal, bran, rice bran, brown sugar and the like.Functional compound additives include pre-embedded material and microcapsule coated sodium butyrate, and the pre-embedded material is prepared by embedding treatment of astaxanthin, lutein and rosemary extract with β-cyclodextrin and gum arabic.The application significantly reduces the incidence of intestinal inflammation of California bass through the synergistic effect of fermented plant protein, pre-embedded antioxidant combination and microcapsule sodium butyrate, enhances antioxidant capacity, improves body color, improves the stability of active ingredients and feeding rate, and realizes low morbidity under antibiotic-free breeding conditions.

The application discloses a culture method for enhancing the body color of Eaststar Prawn and relates to the technical field of aquaculture. The method comprises the following steps: (1) light induction: white-red-blue-yellow light sources are alternately used for irradiation, (2) color enhancer feeding: the color enhancer is fed simultaneously with the basic feed, and the components of the color enhancer include Camellia nitidissima polysaccharide, Sargassum fusiforme polysaccharide, fucoxanthin, scallop peptide, silymarin, guanidino acetic acid and sodium butyrate, and (3) water flow stimulation. Through the regulation of physiological metabolism, nutrient absorption and environmental adaptability, the method jointly acts on the body color performance of Eaststar Prawn, is favorable for enhancing the body color of Eaststar Prawn and improving the economic value of Eaststar Prawn.

The invention discloses an efficient adenovirus packaging method, and belongs to the technical field of genetic engineering. The method comprises the following steps: culturing and packaging cells; aAV carrier plasmids, AAV packaging plasmids and adenovirus helper plasmids are mixed according to an optimized molar ratio (preferably 1: 1: 1.2) with high molecular weight polyethyleneimine (PEI) according to an optimized mass ratio (preferably 3: 1) to form a transfection compound for transfecting cells; after transfection, adding sodium butyrate and reducing the culture temperature to synergistically promote virus packaging and accumulation. According to the method, transfection key parameters and culture conditions are systematically optimized, the AAV packaging efficiency and the total virus yield are remarkably improved, the production cost is reduced, and the method is stable in process, easy to amplify and suitable for large-scale production of AAV gene therapy drugs.

The invention discloses an application of butyric acid or a derivative thereof in preparation of a zearalenone reproductive toxicity resisting preparation. Also disclosed is an antitoxic feed composition comprising a specific base ration and a butyric acid derivative. In-vivo and in-vitro tests and molecular mechanism researches prove that butyric acid and derivatives thereof (such as tributyrin and sodium butyrate) can effectively relieve zearalenone (ZEN)-induced animal ovary injury. The action mechanism of the traditional Chinese medicine composition is that follicular atresia is reduced, the balance of reproductive hormones (such as GnRH, E2, AMH and P4) is recovered, and clinical symptoms caused by ZEN poisoning such as vulva swelling and the like are relieved by activating a BMPs / SMAD signal channel, especially up-regulating SMAD4 protein expression and inhibiting oxidative stress (ROS) and Caspase-3 mediated granular cell apoptosis.

The present invention relates to an in-vitro transduction enhancer and a method for detecting infection efficacy, and particularly, provided is an in-vitro transduction enhancer which comprises one or more of neuraminidase (NA), sodium butyrate (NaBu) and Poly-Lysine. It is found for the first time that the enhancer can greatly reduce a usage amount of viruses or vectors (such as viral vectors) and has a better efficacy detection effect, so that a technical bottleneck is broken through, and a technical obstacle in the art is solved.

The invention discloses a protamine mediated fluorescence resonance energy transfer (FRET) sensing system and application thereof. According to the protamine, a tetraphenylethylene-derived anionic molecule (4, 4 '-(((1, 2-stilbene-1, 2-disubunit) bis (4, 1-phenyl)) bis (aza-disubunit)) bis (4-sodium oxobutyrate)) is electrostatically enriched on the surface of the protamine, and a tetraphenylethylene group emits fluorescence after being aggregated and acts as an energy donor; furthermore, cationic dye (4-(4-dimethylaminostyryl)-1-methyl iodide pyridine) is added as an energy receptor, and a fluorescence resonance energy transfer (FRET) system is constructed. Through construction of the FRET system, long-wavelength fluorescence emission can be obtained, high-sensitivity detection of trypsin and screening of enzyme preparations are achieved, and the FRET system is expected to be applied to complex media.

The application discloses a pig beta-defensin-3 gene core promoter and a construction method and application thereof, and belongs to the technical field of genetic engineering. The sequence of the core promoter is shown as SEQ ID No. 1, the core promoter has significant promoter activity in pig small intestinal epithelial cells, and can be regulated by nutrients such as sodium butyrate and glutamine. The application also discloses a construction method for specifically amplifying the core promoter, which comprises the following steps: performing PCR amplification by taking pig small intestinal epithelial cell DNA as a template, taking the upstream primer and the downstream primer shown as SEQ No. 2-SEQ No. 3 as the primer, constructing a pMD-18T-pBD-3-P recombinant plasmid, and taking the recombinant plasmid as a template to amplify a core promoter fragment. The core promoter provides an excellent experimental system for studying the transcriptional regulation mechanism of the pig beta-defensin-3 gene, the nutrient response characteristics provide a new platform for studying the interaction between nutrition and immunity, and the core promoter has a wide application prospect in the field of healthy breeding of livestock and poultry.

The present invention provides compositions for use in the prevention and / or treatment of infections caused by rhinovirus. [Solution] A composition comprising sodium butyrate for the prevention and / or treatment of viral infections caused by rhinovirus. The composition may further contain vitamin D. The composition may be administered in the form of tablets, capsules, or inhalers. Alternatively, it may be provided as a dietary supplement.

The invention provides a pet intestinal health nutritional supplement as well as a preparation method and application thereof, aiming at the defect that the existing pet feed lacks an intestinal directional regulation function, a gel-fiber two-phase network is innovatively constructed to realize partitioned controlled release of active ingredients, and a colloid is formed by xylooligosaccharide / flaxseed gum, so that the health of the intestinal health nutritional supplement is realized. Embedding probiotics, sodium butyrate and glutamine; a fiber network is formed by spruce fibers and apple fibers, glucose oxidase, Chinese herbal medicine extracts and ROS response type anti-inflammatory nanoparticles are physically adsorbed, oligochitosan / rosmarinic acid-Fe < 2 + > serves as a packing material of the nanoparticles, lysozyme serves as a core material, and chelating bond breakage is triggered in the high ROS environment at the inflammation site to achieve targeted drug release. According to the pet intestinal health nutritional supplement, through a three-stage delivery mechanism of stomach protection, small intestine slow release and colon targeting, the pet intestinal environment is adjusted, and the improvement effect on acute or chronic intestinal inflammation is improved.

This invention provides a stem cell preparation for skin repair and its application, belonging to the field of biomedical technology. The stem cell preparation is obtained by inducing stem cells in a composite culture medium containing sodium butyrate, L-carnosine, and quercetin, followed by short-term pulsed hypoxia treatment, ultrasonic disruption, deep freeze-thaw rehydration, and vacuum freeze-drying to obtain a lyophilized stem cell powder. Experimental results show that the stem cell preparation of this invention significantly promotes the proliferation of human skin fibroblasts and significantly promotes the migration of human keratinocytes. In a mouse full-thickness skin defect model, local application after reconstitution in PBS buffer containing 0.1% sodium hyaluronate (concentration ≥100 μg / mL) significantly accelerates wound closure. In summary, the preparation of this invention exhibits high activity, strong stability, and broad prospects for clinical translation.

The invention discloses a method for improving pig somatic cell nucleus embryo transfer efficiency, and belongs to the technical field of pig somatic cell nucleus transfer. The method comprises the following steps: treating a reconstructed embryo subjected to pig somatic cell nuclear transfer by using sodium butyrate to improve the acetylation level of the reconstructed embryo, or overexpressing KDM2A to reduce the methylation level of the reconstructed embryo so as to improve the efficiency of transferring the embryo by using the pig somatic cell nuclear transfer. According to the present invention, the research results show that the high enrichment of the ZGA gene promoter region H3K36me2 and the enrichment reduction of the far-end cis-regulatory element H3K27ac result in the existing unrepresented epigenetic disorder, such that the SCNT efficiency is limited; therefore, the H3K36me2 is deleted through the demethylase KDM2A, or the HDAC for inhibiting the deacetylation of the H3K27ac in a NaB-mediated manner is used, so that the development arrest of the SCNT embryo is relieved, and the blastocyst forming efficiency is improved.

The invention discloses application of sodium butyrate in preparation of a medicine for preventing or treating sepsis-related acute kidney injury, and relates to the technical field of pharmacy. The invention relates to an application of sodium butyrate in preparation of a medicine for preventing or treating sepsis-related acute kidney injury. The invention finds and verifies that sodium butyrate improves the kidney function of sepsis-related acute kidney injury, alleviates cell inflammatory response and oxidative injury and inhibits ferroptosis. The sodium butyrate inhibits ferroptosis by increasing the expression of VDR and GPX4 proteins.

The invention belongs to the technical field of feed product preparation, and particularly relates to a composite functional feed additive and application thereof. The feed contains a compound functional feed additive, and the compound functional feed additive comprises the following components: 1-2 parts of Vc-phosphate, 1-2 parts of taurine, 1-2 parts of betaine, 0.4-0.5 part of bile acid, 0.4-0.5 part of mannan oligosaccharide, 0.1-0.2 part of beta-glucan, 0.4-0.5 part of saccharomyces cerevisiae cell wall, 1-2 parts of sodium butyrate and 8-10 parts of a compound traditional Chinese medicine preparation. The compound traditional Chinese medicine preparation contains scutellaria baicalensis, rheum officinale, golden cypress, astragalus membranaceus, folium isatidis, houttuynia cordata, isatis root, radix paeoniae alba, radix bupleuri and gynostemma pentaphylla. Through combined use of the functional feed additive, the feed utilization efficiency of the largemouth bass and the ictalurus punctatus juvenile fish can be effectively improved, and the physique is enhanced, so that the growth performance is improved, and the culture benefit is improved.

The current invention is directed to a method for ameliorating or treating a metabolic syndrome-related disease or disorder in a subject in need thereof, including administering to the subject a therapeutically effective amount of a composition including L-arginine and sodium butyrate.

The invention belongs to the technical field of feed additives, and discloses a compound functional feed additive for pelteobagrus fulvidraco as well as a preparation method and application of the compound functional feed additive. The invention creatively provides a multi-target synergistic compound functional feed additive for pelteobagrus fulvidraco by scientifically compounding the antibacterial peptide, the eucommia ulmoides extract, the eucalyptus essential oil and the sodium butyrate and supplementing a carrier. The composite functional feed additive is not simply superposed in functions of all the components, but systematically improves the health level and disease resistance of the pelteobagrus fulvidraco from multiple aspects of pathogen inhibition, immunoregulation, oxidative stress resistance, intestinal barrier repair and the like through a synergistic effect, and can replace or reduce the use of antibiotics.

The invention belongs to the technical field of aquaculture, and particularly relates to an aquatic population breeding and seedling raising method based on water temperature-hormone cooperative regulation and control and application of the aquatic population breeding and seedling raising method based on water temperature-hormone cooperative regulation and control. The growth regulator is used for enhancing the reproduction promoting effect of luteinizing hormone releasing hormone or analogues thereof; propagation promotion refers to promotion of oocyte growth, promotion of yolk accumulation, shortening of ovary development cycle and improvement of oviposition rate. The invention discovers and verifies the fish breeding promoting effect of the butyric acid and the sodium salt thereof for the first time, wherein the butyric acid and the sodium salt thereof can enhance luteinizing hormone release hormone.