22 results about "Rat brain" patented technology

The invention provides a pharmaceutical composition containing polypeptide and application of the pharmaceutical composition to preparation of a medicine for preventing and treating Parkinson's disease, and belongs to the technical field of polypeptide and biology. The amino acid sequence of the polypeptide is PCVLYDLREKM, the damage of 6-OHDA induced BZ555 nematode dopaminergic neurons can be obviously relieved, mouse dyskinesia caused by a Parkinson's disease model can be obviously improved, the spontaneous standing frequency can be improved, the movement speed in an open field can be increased, and meanwhile the IL-1beta and IL-18 levels caused by the mouse Parkinson's disease model can be reduced; the polypeptide provided by the invention can significantly reduce MAO-B level while significantly increasing DA level in nigra of brain tissues of rats with Parkinson's disease, which prompts that the polypeptide provided by the invention has a significant therapeutic effect on Parkinson's disease, and has a wide market prospect in many fields such as health food and medicine.

The utility model relates to an electrode for collecting mouse brainolfactory globules, which comprises an electrode body, a heat shrink tube, a circuit board, a connector and a protective sleeve, the electrode body is provided with 16 groups of electrodes, the 16 groups of electrodes form a cluster, each group of electrodes has four wires, a lead is arranged in the circuit board and is connected with the electrode body, and the connector is connected with the protective sleeve. The tail end of the circuit board is provided with a connector which is connected with a 64-pin plug, the other end of the 64-pin plug is connected with detection equipment, a heat shrink tube is arranged between the electrode body and the circuit board, and a protective sleeve is sleeved outside the electrode body. According to the utility model, biological signals of mouse brainolfactory globules can be effectively collected, and the mouse brainolfactory globules can be used for special purposes through data comparison. When in use, the electrode body is in contact with the mouse olfactory bulb to collect biological signals, so that researchers are helped to give mouse instructions, a special purpose is achieved, and the researchers are helped to overcome some hidden fields. The structure is simple, and operation is convenient.

The application discloses a small animal electric treadmill device with electromagnetic shielding, which comprises a treadmill box body made of aluminum alloy material, wherein a runway box body is arranged at the front part, a controller box body is arranged at the rear part, and the runway box body and the controller box body are separated by an insulating material plate, the application effectively reduces electromagnetic interference in the process of collecting rat brain electricity, the designed electric stimulation module can reduce the damage to the limbs (feet) of the rat and the influence on the collection of brain electricity, two motors are used to drive left and right runways respectively, the comparison experiment of different speed requirements can be realized, the setting of the runway slope can increase the running difficulty and meet various training modes, the built-in camera can record the state of the rat when running and the posture of each part, and the design of the running belt, the runway and the cover plate can prevent the damage to the rat in the experiment.

The invention discloses an experimental method for relieving ferroptosis of cerebral apoplexy rats through tVNS, and relates to the technical field of nerve regulation and control. Comprising the following steps: S1, constructing a rat MCAO model, and constructing an SD rat right cerebral ischemia reperfusion model by adopting a suture method; s2, experiment grouping: randomly dividing experimental animals into (1) a false operation group: an SHAM group; (2) a cerebral ischemia reperfusion group: an MCAO group; (3) an ear vagus nerve electrical stimulation group after cerebral ischemia reperfusion: a tVNS group; and S3, performing ear vagus nerve electrical stimulation intervention. Ferroptosis related proteins such as GPX4, ACSL4 and FTH1 are detected through Western blot, it is proved that tVNS can improve stroke rat brain tissue ferroptosis, and clear molecular mechanism support is provided for intervention; the content of reduced glutathione and the content of malondialdehyde are respectively detected by using a GSH and MDA content detection kit, and the effectiveness of tVNS in the aspect of relieving ferroptosis is further verified.

The application belongs to the technical field of medicine, and particularly relates to a muscone derivative, a preparation method and application thereof. The muscone derivative cyclopentadecanedione monoxime has obvious advantages over muscone and other derivatives in improving the MCAO rat mNSS score, microcirculation blood flow, reducing the cerebral infarction rate, the cortical and hippocampal neuron necrosis rate, and the brain water content, and increasing the SOD and CAT contents of the rat brain tissue which are abnormally reduced due to ischemia, and is a potential effective drug for cerebral ischemia-reperfusion injury.

The invention relates to the technical field of biology, in particular to a method for verifying influence of quercetin on HIE newborn rat lymphatic systems and neurological functions. The method comprises the following steps: a, acquiring a newborn rat for an experiment, and establishing an HIE model; b, preparing a quercetin working solution; c, after a rat in the HIE model is selected and injected with a quercetin working solution, nerve function evaluation is completed, then the brain of the rat is taken out to be sliced, TTC dyeing is completed, and the infarct area percentage is calculated; and performing brain water content detection on the rats to complete verification of influence of quercetin on lymphatic system functions in HIE. The method provided by the invention has the advantages that the steps are simple, and the influence of the quercetin on the lymphatic system and the neurological function of the HIE newborn rat can be more intuitively verified.

The invention belongs to the technical field of precise driving and positioning, and relates to a high-precision piezoelectric micro-driving device for synchronously recording rat multi-brain-region neural signals, which mainly comprises a piezoelectric stack driving element, a thermal shrinkage and cold expansion material clamping element, a rectangular flexible hinge, a neural electrode and a packaging shell. The driving element can provide a stepping displacement output power source for the neural electrode, the clamping elements are embedded into an upper PCB and a lower PCB in pairs and can clamp and release the neural electrode, and the flexible hinge can regulate and control the stepping displacement output characteristic of the neural electrode. The piezoelectric micro-driving device independently translates a plurality of neural electrodes by cooperatively controlling a driving element and a clamping element under the excitation of a time sequence signal, and is accurately implanted into a target brain region of a rat to synchronously record neural signals of multiple brain regions. The device is simple and compact in structure, small and light, easy to integrate and miniaturize, capable of being used for accurate, independent and rapid micron-level positioning of multiple neural electrodes, and beneficial for achieving high-precision, multi-brain-area and large-scale synchronous recording of rat electroencephalogram signals.

The invention relates to the technical field of neuroscience, in particular to a craniotomy method for obtaining complete rat brain tissue, which comprises two parts of early-stage preparation and skull opening. The early-stage preparation comprises anesthesia, scalp incision and fascia cleaning. The skull opening sequence is sagittal seam cracking, top seam cracking and partial skull opening in sequence. Refined operation design is performed according to skull anatomy, so that the method is remarkably superior to the prior art in the aspects of integrity, operation efficiency and applicability of obtained brain tissues, and reliable technical support is provided for neuroscience research.

The application belongs to the technical field of animal model construction, and discloses a method for establishing a tumor-bearing animal model for an OCT system, wherein a SD rat supratentorial brain parenchymal glioma model, a SD rat chiasma glioma model and a SD rat brain stem glioma model are constructed by using a C6 cell line; a rat sciatic nerve sheath tumor model and a rat trigeminal nerve RT4 tumor model are constructed by using an RT4 cell line to obtain a SD rat RT4 tumor cell animal model; and a BALB / c nude mouse U87MG tumor cell animal model and a BALB / c nude mouse IOMM-Lee tumor cell animal model are constructed by using a U87MG cell line and an IOMM-Lee cell line, respectively. The application successfully constructs a rat brain glioma model, a chiasma glioma model, a trigeminal nerve sheath tumor model, a sciatic nerve sheath tumor model, a nude mouse brain glioma model and a nude mouse sciatic nerve meningioma model.

The invention discloses application of a compound high spot-nourishing tablet in preparation of a medicine for treating cerebral ischemia-reperfusion injury. A rat cerebral ischemia reperfusion injury model is established through middle cerebral artery embolism, and serum, pathology and infarction areas of rats are observed and detected. Results show that the neurological score of the model group ZeaLonga is significantly increased, pathological changes are large, proinflammatory factors TNF-alpha, IL-6 and IL-1beta are significantly increased, oxidation promoting MDA is significantly increased, and antioxidant SOD expression is significantly increased. And compared with a model group, the weight, the neurological score and the survival rate are obviously improved. The results of HE staining, TTC staining and TUNEL staining show that the nerve cell morphology, arrangement, infarct area and cell apoptosis condition of each administration group are obviously improved. The application provided by the invention can improve the behavior of rats, reduce the infarct area, protect nerve cells and reduce the apoptosis of ischemic regions; by reducing inflammation and oxidation in rat brain tissues, damage of inflammation and oxidative stress to the rat brain tissues is reduced.

The invention belongs to the technical field of neuroscience research, and particularly relates to a mouse brain embedding box for neuroscience research and a low-temperature storage device thereof.The mouse brain embedding box comprises a storage cabinet, a separation assembly arranged in the storage cabinet, a refrigeration assembly arranged in the storage cabinet, and a plurality of placement assemblies arranged in the storage cabinet; the multiple placement assemblies are located at the tops of the multiple second partition plates correspondingly, and multiple gas storage assemblies are arranged in the storage cabinet. According to the invention, the embedding box is independently stored in the storage drawer, overall exposure is avoided when the single embedding box is stored and taken, cold air leakage is effectively reduced, cold air is stored in the air storage box through the air storage assembly when the storage drawer is pulled out, and cold air in the air storage box is sent back to the storage drawer through the air storage assembly when the storage drawer retracts; cold air can be recycled, and the effect of reducing cold air waste is achieved; the problem that unnecessary energy waste is likely to be caused in the process of taking the embedding box of an existing refrigeration device is solved.

The utility model discloses a body fixing device for a rat brain stereotaxic apparatus, and relates to the technical field of animal experiments, the body fixing device comprises a bottom plate, the top end of the bottom plate is fixedly connected with a fixing frame, the top end of the fixing frame is rotatably connected with a fixing mechanism, and the fixing mechanism comprises a motor; the device comprises a fixing frame, two fixing blocks are fixedly connected to the top end of the fixing frame, the two fixing blocks are jointly and rotationally connected with a long rod in a sleeving mode, first gears are fixedly connected to the two ends of the two long rods correspondingly, second gears are connected to the outer surfaces of the two first gears in an engaged mode correspondingly, and the opposite ends of the two second gears are jointly and fixedly connected with a connecting rod; the output end of the motor is fixedly connected with one of the second gears, a cover plate is fixedly connected to the outer surface of the long rod, and fixing grooves are formed in the bottom end of the cover plate and the top end of the fixing frame, the device can limit movement of a rat by arranging the fixing mechanism, and therefore the positioning accuracy can be effectively improved.

The invention discloses application of clomastine in treatment of traumatic brain injury. By constructing a rat TBI model, detecting related pathway protein change conditions in rat brain injury models of different days through WB, and detecting demyelination conditions through an electron microscope, it is proved that clomastine can improve endoplasmic reticulum stress and iron content change conditions, has a neuroprotective effect, and can be used for treating neurological diseases. And a foundation is laid for developing a new medicine for treating traumatic brain injury.

The invention provides a method for simultaneously detecting nicotine and metabolites, neurotransmitters and lipids thereof in a brain tissue sample, which comprises the following steps: injecting nicotine into the abdominal cavity of a rat to obtain the brain tissue of the rat, and treating to prepare a brain tissue slice; then putting the brain tissue slice into a mass spectrum imaging instrument for detection; and analyzing the spatial distribution characteristics of nicotine and metabolites, neurotransmitters and lipids thereof in the brain after the rat intraperitoneal injection of nicotine on the basis of the obtained rat brain mass spectrum imaging graph. Wherein the spray voltage of the mass spectrum imaging instrument is 3.5-6.0 kV, and the spray solvent is formed by mixing acetonitrile, isopropanol and water according to the volume ratio of (5-7): (1-3): (1-3). The simultaneous detection method can accurately obtain spatial distribution and metabolism information of the three substances in the brain tissue, has the characteristics of simplicity and convenience in operation, good accuracy, high precision and the like, and provides an accurate and effective analysis method for spatial distribution and metabolism research of nicotine and metabolites thereof, neurotransmitters and lipids in the brain tissue.

The invention relates to the field of biomedicine, and discloses a capillary segment extraction method and application thereof. The method comprises a step of digesting rat brain tissues and a step of separating brain microvascular segments. The method provided by the invention is simple to operate, can be directly realized on wild animals, and does not depend on animal endogenous fluorescent labeling. According to the method, the complete vascular tissue can be obtained, meanwhile, the activity of the cells is reserved, the method is the first method capable of separating the complete vascular tree from the brain tissue and then further researching the functions of all the cells on the vascular tissue, and the method has important significance on research of the brain microvascular segment.

The invention relates to the field of experimental instruments, and discloses a mouse brain bilateral optical fiber implantation device which comprises an operation platform and further comprises an optical fiber clamping mechanism and a mouse head restraining mechanism which are installed on the operation platform, the optical fiber clamping mechanism is used for clamping and fixing an optical fiber, and the mouse head restraining mechanism is used for fixing the head of a mouse; the optical fiber clamping mechanism comprises a lifting platform installed on an operation platform; the vertical sliding block is arranged on the lifting platform, and the lifting platform is connected with the vertical sliding block through a driving component. An optical fiber can be directly and rigidly clamped through the optical fiber fixator of the optical fiber clamp, the horizontal position and the inclination angle of the optical fiber can be locked through the connection sliding block self-locking piece and the connection sleeve fastening piece of the dial, and it is ensured that the optical fiber is kept in a stable posture in the whole implanting process; the outer surface of the horizontal guide rod is marked with a symmetrical dial, and the horizontal distance between the optical fibers on the two sides can be accurately controlled.

The application provides a rat brain natriuretic peptide (BNP) detection kit and application thereof. A rat BNP monoclonal antibody specific to rat BNP is prepared and obtained by taking rat BNP as an immunogen, the rat BNP monoclonal antibody has good specificity and affinity, and after the rat BNP monoclonal antibody is prepared into an ELISA detection kit, the rat BNP monoclonal antibody can be effectively used for content determination of rat BNP, and has good application prospect.

The application discloses a kind of brain tissue and blood plasma double matrix amino acid class matter pretreatment and detection method, the method includes the following steps: standard preparation;Differential sample preparation and homogenization;Double protein precipitation and preliminary purification;Solid phase extraction (SPE) depth purification and enrichment;Microenvironment control's pre-column derivatization;HPLC-FLD chromatographic separation and detection;Result calculation.The application is by optimizing sample pretreatment process, introduce SPE depth purification and pre-column derivatization technology under specific pH condition, effectively solve the problem of brain tissue and blood plasma matrix complex, many interferents and amino acid derivatization efficiency low.This method has the advantages of simple steps, high accuracy, good sensitivity and excellent reproducibility, can realize the rapid, synchronous detection of 13 kinds of amino acid class matters in rat brain tissue and plasma, provides reliable technical means for neurotransmitter and related metabolite research.

The invention belongs to the technical field of biological pharmacy, and provides application of glycyrrhizin in preparation of a medicine for treating white matter injury. Animal experiment results show that the HMGB1 inhibitor Gly can obviously improve rat WMI pathological changes, reduce rat brain white matter region HMGB1 expression, obviously reduce the number of rat brain white matter region NG2 + Olig2 + double positive cells and improve rat WMI afterbrain white matter region OLs differentiation disorder; the myelination disorder of the white matter region of the rat after WMI can be improved, and the number of myelination axons of the rat is obviously increased; and the learning ability and the spatial memory ability of the rats are improved. It is clear that Gly can inhibit expression of HMGB1, and an effective technical means is provided for treatment of white matter damage.

The invention discloses an application of an agilawood extract in preparation of a medicine for resisting cerebral arterial thrombosis, and belongs to the technical field of traditional Chinese medicines. A cerebral arterial thrombosis rat model is constructed by utilizing a 2-VO method, the action mechanism of the agilawood extract on cerebral arterial thrombosis (IS) is researched by analyzing the physiological status, behavioral performance and brain tissue pathological change of rats, the treatment effect of agilawood on cerebral arterial thrombosis is further evaluated, and a theoretical basis is provided for the action mechanism of agilawood. Results show that the agilawood extract can improve physiological status, balance ability and neurological function damage of IS rats; hE staining results show that the agilawood extract can improve pathological states such as abnormal neuron arrangement and neuronal nucleus fixation and contraction of IS rat brain hippocampus. And a theoretical basis is laid for treating behavioral diseases of cerebral arterial thrombosis patients and improving the life quality of the cerebral arterial thrombosis patients.

The utility model discloses a mouse head fixing device for mouse brain separation, and relates to the technical field of biological research. Comprising an operation plate, a liquid groove is formed in the operation plate, two pairs of supporting legs are arranged on the lower wall face of the operation plate, a U-shaped cavity and an open groove are formed in the operation plate, two-way lead screws are rotationally connected into the two side wall faces of the U-shaped cavity, a sliding rod is connected between the two side wall faces of the U-shaped cavity, and a rotary knob is rotationally connected into the operation plate. The rotary knob is connected with the two-way lead screw, the two-way lead screw is in threaded connection with a pair of moving plates, and the pair of moving plates are both installed on the sliding rod in a sliding mode. According to the auxiliary craniotomy device, a structure for assisting craniotomy is designed, and the auxiliary craniotomy device can be provided with a structure for clamping a fur layer, so that the influence of the fur layer on craniotomy is avoided, the operation of manually fixing the fur layer is reduced, an operator can finish craniotomy more intensively, and the integrity of rat brain separation is improved.

The invention discloses a standardized mouse brain region Western blot accurate sampling device and method. The device comprises a metal table, a slicing region and a segmentation region, a cavity is formed in the metal table, and refrigerant is injected into the cavity; a groove is formed in the slicing area; the segmentation area comprises an orthogonal cutting unit and a skew cutting unit, and the orthogonal cutting unit and the skew cutting unit are used for realizing accurate cutting of the brain area of the target mouse. The method comprises the steps of target positioning, low-temperature oxygen-enriched cerebrospinal fluid perfusion, brain tissue treatment, cutting, storage and the like. Through cooperation of the device and the method, standardized, anti-degradation and high-precision material taking is realized, and the experiment repeatability and the result reliability are improved.