10 results about "Rat brain" patented technology

The invention provides a pharmaceutical composition containing polypeptide and application of the pharmaceutical composition to preparation of a medicine for preventing and treating Parkinson's disease, and belongs to the technical field of polypeptide and biology. The amino acid sequence of the polypeptide is PCVLYDLREKM, the damage of 6-OHDA induced BZ555 nematode dopaminergic neurons can be obviously relieved, mouse dyskinesia caused by a Parkinson's disease model can be obviously improved, the spontaneous standing frequency can be improved, the movement speed in an open field can be increased, and meanwhile the IL-1beta and IL-18 levels caused by the mouse Parkinson's disease model can be reduced; the polypeptide provided by the invention can significantly reduce MAO-B level while significantly increasing DA level in nigra of brain tissues of rats with Parkinson's disease, which prompts that the polypeptide provided by the invention has a significant therapeutic effect on Parkinson's disease, and has a wide market prospect in many fields such as health food and medicine.

The application discloses a small animal electric treadmill device with electromagnetic shielding, which comprises a treadmill box body made of aluminum alloy material, wherein a runway box body is arranged at the front part, a controller box body is arranged at the rear part, and the runway box body and the controller box body are separated by an insulating material plate, the application effectively reduces electromagnetic interference in the process of collecting rat brain electricity, the designed electric stimulation module can reduce the damage to the limbs (feet) of the rat and the influence on the collection of brain electricity, two motors are used to drive left and right runways respectively, the comparison experiment of different speed requirements can be realized, the setting of the runway slope can increase the running difficulty and meet various training modes, the built-in camera can record the state of the rat when running and the posture of each part, and the design of the running belt, the runway and the cover plate can prevent the damage to the rat in the experiment.

The application belongs to the technical field of medicine, and particularly relates to a muscone derivative, a preparation method and application thereof. The muscone derivative cyclopentadecanedione monoxime has obvious advantages over muscone and other derivatives in improving the MCAO rat mNSS score, microcirculation blood flow, reducing the cerebral infarction rate, the cortical and hippocampal neuron necrosis rate, and the brain water content, and increasing the SOD and CAT contents of the rat brain tissue which are abnormally reduced due to ischemia, and is a potential effective drug for cerebral ischemia-reperfusion injury.

The invention discloses application of clomastine in treatment of traumatic brain injury. By constructing a rat TBI model, detecting related pathway protein change conditions in rat brain injury models of different days through WB, and detecting demyelination conditions through an electron microscope, it is proved that clomastine can improve endoplasmic reticulum stress and iron content change conditions, has a neuroprotective effect, and can be used for treating neurological diseases. And a foundation is laid for developing a new medicine for treating traumatic brain injury.

The invention provides a method for simultaneously detecting nicotine and metabolites, neurotransmitters and lipids thereof in a brain tissue sample, which comprises the following steps: injecting nicotine into the abdominal cavity of a rat to obtain the brain tissue of the rat, and treating to prepare a brain tissue slice; then putting the brain tissue slice into a mass spectrum imaging instrument for detection; and analyzing the spatial distribution characteristics of nicotine and metabolites, neurotransmitters and lipids thereof in the brain after the rat intraperitoneal injection of nicotine on the basis of the obtained rat brain mass spectrum imaging graph. Wherein the spray voltage of the mass spectrum imaging instrument is 3.5-6.0 kV, and the spray solvent is formed by mixing acetonitrile, isopropanol and water according to the volume ratio of (5-7): (1-3): (1-3). The simultaneous detection method can accurately obtain spatial distribution and metabolism information of the three substances in the brain tissue, has the characteristics of simplicity and convenience in operation, good accuracy, high precision and the like, and provides an accurate and effective analysis method for spatial distribution and metabolism research of nicotine and metabolites thereof, neurotransmitters and lipids in the brain tissue.

The invention relates to the field of biomedicine, and discloses a capillary segment extraction method and application thereof. The method comprises a step of digesting rat brain tissues and a step of separating brain microvascular segments. The method provided by the invention is simple to operate, can be directly realized on wild animals, and does not depend on animal endogenous fluorescent labeling. According to the method, the complete vascular tissue can be obtained, meanwhile, the activity of the cells is reserved, the method is the first method capable of separating the complete vascular tree from the brain tissue and then further researching the functions of all the cells on the vascular tissue, and the method has important significance on research of the brain microvascular segment.

The application discloses a kind of brain tissue and blood plasma double matrix amino acid class matter pretreatment and detection method, the method includes the following steps: standard preparation;Differential sample preparation and homogenization;Double protein precipitation and preliminary purification;Solid phase extraction (SPE) depth purification and enrichment;Microenvironment control's pre-column derivatization;HPLC-FLD chromatographic separation and detection;Result calculation.The application is by optimizing sample pretreatment process, introduce SPE depth purification and pre-column derivatization technology under specific pH condition, effectively solve the problem of brain tissue and blood plasma matrix complex, many interferents and amino acid derivatization efficiency low.This method has the advantages of simple steps, high accuracy, good sensitivity and excellent reproducibility, can realize the rapid, synchronous detection of 13 kinds of amino acid class matters in rat brain tissue and plasma, provides reliable technical means for neurotransmitter and related metabolite research.

The invention belongs to the technical field of biological pharmacy, and provides application of glycyrrhizin in preparation of a medicine for treating white matter injury. Animal experiment results show that the HMGB1 inhibitor Gly can obviously improve rat WMI pathological changes, reduce rat brain white matter region HMGB1 expression, obviously reduce the number of rat brain white matter region NG2 + Olig2 + double positive cells and improve rat WMI afterbrain white matter region OLs differentiation disorder; the myelination disorder of the white matter region of the rat after WMI can be improved, and the number of myelination axons of the rat is obviously increased; and the learning ability and the spatial memory ability of the rats are improved. It is clear that Gly can inhibit expression of HMGB1, and an effective technical means is provided for treatment of white matter damage.

The utility model discloses a mouse head fixing device for mouse brain separation, and relates to the technical field of biological research. Comprising an operation plate, a liquid groove is formed in the operation plate, two pairs of supporting legs are arranged on the lower wall face of the operation plate, a U-shaped cavity and an open groove are formed in the operation plate, two-way lead screws are rotationally connected into the two side wall faces of the U-shaped cavity, a sliding rod is connected between the two side wall faces of the U-shaped cavity, and a rotary knob is rotationally connected into the operation plate. The rotary knob is connected with the two-way lead screw, the two-way lead screw is in threaded connection with a pair of moving plates, and the pair of moving plates are both installed on the sliding rod in a sliding mode. According to the auxiliary craniotomy device, a structure for assisting craniotomy is designed, and the auxiliary craniotomy device can be provided with a structure for clamping a fur layer, so that the influence of the fur layer on craniotomy is avoided, the operation of manually fixing the fur layer is reduced, an operator can finish craniotomy more intensively, and the integrity of rat brain separation is improved.

The invention discloses a standardized mouse brain region Western blot accurate sampling device and method. The device comprises a metal table, a slicing region and a segmentation region, a cavity is formed in the metal table, and refrigerant is injected into the cavity; a groove is formed in the slicing area; the segmentation area comprises an orthogonal cutting unit and a skew cutting unit, and the orthogonal cutting unit and the skew cutting unit are used for realizing accurate cutting of the brain area of the target mouse. The method comprises the steps of target positioning, low-temperature oxygen-enriched cerebrospinal fluid perfusion, brain tissue treatment, cutting, storage and the like. Through cooperation of the device and the method, standardized, anti-degradation and high-precision material taking is realized, and the experiment repeatability and the result reliability are improved.