128 results about "Rat brain" patented technology

The invention discloses a robot simulated navigation method based on rat brain-hippocampal navigation, and belongs to the technical field of robot motion navigation. The method is characterized by that a robot CPU (Central Processing Unit) predefines a grid cell layer cell layer G and a place cell layer P by imitating rat brain and hippocampal tissues, motion information including a motion direction phi and a velocity v is collected when a robot explores an environment, the collected motion information is input into the grid cell layer, the motion information is integrated by using a shock interference model in the grid cell layer to form a grid cell layer response, a connection weight value W of the grid cell layer cell layer G and a place cell layer P is calculated by using a neural network algorithm to form a location cell-grid cell response network, and the robot perception for a space namely is stored in the form of a neural network response. The robot controls a motion decision module to implement motion decision-making according to space information stored in a cognitive map, and the navigation method disclosed by the invention independently obtains a better spatial cognitive effect during an exploration motion of the robot, and can be applied to industrial robots, service robots and the like.

The invention discloses an in-vitro separation culture method for hippocampal neurons of an adult rat. The in-vitro separation culture method comprises the following steps of coating a culture plate; separating a rat brain; separating a hippocampus; preparing a cell suspension; further purifying the neurons; carrying out cell inoculation; and carrying out in-vitro primary culture. The invention aims at providing a high-purity and high-activity acquiring (separating and purifying) method for hippocampal neurons and an establishing method for a flow culture system suitable for long-term in-vitro culture of hippocampal neurons of the adult rat.

The invention discloses an experiment method for studying the effect of tenuigenin on synaptic transmission of rat hippocampal neuron. The method adopts a micro control instrument, a microscopic, and a recording electrode to monitor the spontaneous current state of neuron in tenuigenin with different concentrations, and then the monitor current data is analyzed so as to obtain the influence mechanism and effect of tenuigenin on rat hippocampal neuron. The method adopts a whole-cell patch-clamp technology to research the effect of tenuigenin on spontaneous postsynaptic current in the rat brain hippocampal CA1 area so as to disclose the regulating effect and mechanism of tenuigenin on neuron synaptic transmission, thus the requirements of scientific research and drug development are fulfilled, and at the same time a basis is provided for the further research on the signal transmission mechanism of neuron. The method can be widely used in the pharmaceutical theory researches on neuron, the experiment method and equipment design are scientific and reasonable, and requirements of scientific research can be met.

The invention discloses a micro-fluidic chip for in-vivo on-line simultaneous detection of ascorbic acid and magnesium ion and a preparation method and applications thereof. The micro-fluidic chip comprises: a template with a micro channel and a conductive glass substrate which are connected closely; working electrodes which are disposed at positions corresponding to the micro channel on the substrate in a direction of the micro channel, and are arranged in turn with an amount being the same as the amount of components to be detected; a liquid introducing channel and an inlet are disposed at one port of the micro channel on the template, and a liquid extracting channel and an outlet are disposed at the other port; a counter electrode is disposed at the port of the micro channel where the liquid outlet is disposed; on the template, a reference electrode is disposed at a position corresponding to the micro channel between the main liquid introducing channel and the liquid extracting channel. With the micro-fluidic chip, cross interference between ascorbic acid and magnesium ion during the simultaneous detection is eliminated; furthermore, a micro-dialysis in-vivo on-line detection system is combined so as to realize the simultaneous on-line electrochemical detection of ascorbic acid and magnesium ion in rat brains.

The invention relates to a rat brain section microscopic image segmentation method based on a markov random field theory. Gaussian mixed distribution is trained through the existing tagged image and used in characteristic field modeling, image characteristics are simulated correctly, great guiding significance for random field modeling is obtained, convergence times of an iterative algorithm are greatly reduced, and the accuracy of a segmentation result is improved. In addition, in order to solve the problem that local neighborhood characteristics of a traditional 8 neighborhood pixel model traced image are too rough, the method introduces a pixel gray value and a distance between pixels into a Potts model, a new potential-energy function is defined, image local information is described correctly, and the accuracy of a segmentation result is improved.

The present invention relates to an immortalised human neural precursor cell line, NGC-407. The cell line has been established from human foetal tissue. The cell line has been immortalised using a retroviral vector containing the v-myc oncogene. The cell line is a neural progenitor cell line capable of differentiating into to astrocytes and neurons including dopaminergic neurons. NGC-407 cells are capable of migrating to glioblastoma tumours implanted into rat brains and form gap junctions with the tumour cells. NGC-407 cells expressing a suicide gene can be be used for delivering activated prodrugs in the form of activated nucleoside analogs to tumours.

The invention discloses a micro slow positioning injection device for rat brain experiments. The micro slow positioning injection device comprises a support, a needle pushing mechanism and a needle core pushing mechanism; the support is formed by a bottom plate, retractable supporting columns and a top plate, a fixed platform is also slidably arranged on the bottom plate, and a clamping mechanismis slidably arranged on the fixed platform; the needle pushing mechanism comprises a screw rod, two screw sleeves, two connection rods, a first connection plate and a fixation mechanism, wherein the screw rod is fixedly arranged below the top plate, the screw sleeves are symmetrically arranged on the screw rod, the connection rods are hinged to the screw sleeves, the first connection plate is hinged to both the connection rods, and the fixation mechanism is arranged at the middle of the first connection plate and used for fixing a needle cylinder of an injector; the screw rod is composed of two integrated segments, but the spiral directions of screw threads of the two segments are reverse; the needle core pushing mechanism comprises a pushing boosting cylinder which penetrates through thetop plate and is slidably connected with the top plate, a toothed plate which is fixedly arranged at the top of the pushing boosting cylinder, and a gear which meshes with the toothed plate and can conduct self-locking, the gear is slidably arranged on the top plate, and a first handle and scale lines are arranged on the gear. The micro slow positioning injection device effectively avoids experiment errors caused by long-time manual fixation by a tester, and is reasonable in structure and convenient to operate.

The invention relates to a primary culture method of elderly rat brain vascular endothelial cells. The primary culture method comprises the following steps of firstly, taking out a grey matter part of an elderly rat brain tissue, and making a sample tissue; secondly, putting into a culture dish with a separating medium of fetal calf serum, and carrying out blowing, dispersing and biological enzyme digestion so as to prepare dispersed cells; thirdly, centrifuging so as to prepare a blood capillary; fourthly, re-suspending the blood capillary in the separation medium, and digesting to prepare blood capillary suspension; fifthly, centrifuging, re-suspending the sediment in the separation medium, and carrying out Percoll concentration gradient centrifugation so as to prepare the brain vascular endothelial cells; and sixthly, putting in a culture medium, culturing and carrying out digestion passage or freezing and storing. The primary culture method adopts low-concentration pancreatin or PBS (Phosphate Buffer Solution) digestion cells with 1m M EDTA (Ethylene Diamine Tetraacetic Acid), the cells can be subjected to three times of digestion passage but the characteristics of the brain vascular endothelial cells are still maintained, and the purity and the characteristics of the brain vascular endothelial cells are still maintained after being subjected to freezing and recovery, as a result, a great deal of time and money are saved.

The invention provides a bionic positioning method based on rat brain hippocampus space cells. By means of the method, a robot can achieve the positioning function. The method belongs to the field ofbionics. Firstly, a robot explores the space environment, speed information and direction information are collected through a photoelectric encoder, and a speed cell model and a head orientation cellmodel process the linear speed and the angular speed respectively; then, a bidirectional connection network between the grid cells and the position cells is established, on one hand, the grid cells receive excitability input of an upstream cortex, inhibition input between the grid cells and feedback input of the position cells, and on the other hand, projection information of the grid cells drivesthe position cells to discharge; and finally, an environmental expression calculation model of the position cells is constructed, the correlation of the connection strength between the position cellsand the input cells is calculated, the position cells with the highest correlation with the input cells are selected as final winning cells, the discharge information of the cells is associated withthe position points, and the associated information is stored.