106 results about "Hippocampal neuron" patented technology

The invention discloses an SLAM method based on a rodent model and an RTAB-Map closed-loop detection algorithm. Spatial positioning can be performed by means of hippocampal neurons of the rodent model. A new bionic navigation model is constructed according to an existing closed-loop detection RTAB-MAP algorithm. On the condition that high real-time performance of the system is ensured, correction of a long-term accumulated error in a macroenvironment can be realized, and furthermore low navigation stability caused by indoor light change to a certain extent.

The invention discloses lactobacillus plantarum DP189 and application thereof, and relates to the field of functional food microorganisms. The strain is preserved in China Center for Type Culture Collection on March 25 , 2019 with the preservation number of CCTCC NO:M2019199. By means of the strain, the learning and memory capability of a depressed rat can be improved; the level of brain-derived neurotrophic factors of the depressed rat can be improved; the injury to hippocampal neurons and apoptosis of neuron cells of the depressed rat are reduced; the content of apoptosis proteins JUK2 and Bcl-2 of brain tissue of the depressed rat is lowered; the abundance of verrucomicrobiaceae of the intestinal tract is lowered; the abundance of bacterium lacticum of the intestinal tract is improved, the alpha-diversity of the intestinal floras is improved, and the intestinal flora disturbance of the depressed rat is alleviated. In this way, the lactobacillus plantarum DP189 has good effects of preventing and treating depression / anxiety, infantile autism and inflammatory enteritis. The strain has the anti-depression function, and can serve as a functional probiotic bacterium for preventingand treating the depression, infantile autism and inflammatory enteritis, and a new idea is provided for clinical intervention with and control over depression and other relevant mental diseases.

The invention discloses culture solution for primary culture of newly born rat hippocampal neuron and a method for primarily culturing the rat hippocampal neuron. The culture solution is formed by adding 1,6 diphosphonic acid fructose, fructose and nutrition additive into base culture solution Neurobasalmedium. The method for primarily culturing the rat hippocampal neuron comprises the following steps: 1 preparing hippocampal neuron culture solution; 2 drawing materials and digesting the materials; 3 preparing single cell suspension; and 4 conducting inoculating and cultivating. The method for primarily culturing the rat hippocampal neuron is simple, convenient and capable of obtaining hippocampal neuron with good growth state and high purity by adopting the culture solution and has important application value and economical value.

The invention belongs to the field of cell biology and relates to a method and reagent for the separation and culture of neurons. The method and the reagent are used for solving problems in the prior art, the current in-vitro hippocampal neuron separation and culture methods are improved, and the problem that the proliferation and activity of hippocampal neurons during primary culture can not be maintained is solved. According to the method and the reagent, a relatively mature in-vitro hippocampal neuron culture method is established, the number of obtained nerve cells is sufficient, the growth status is relatively good, and a large number of high-activity hippocampal nerve cells can be separated from mammalian hippocampal tissue, so that the requirements for the primary culture of the hippocampal cells are met, and the demands on experiments of cell biology during neuroscience research can be met.

The present invention provides a novel class of fluorogenic probes for reactive oxygen species. Exemplary probes of the invention utilize a boronate deprotection mechanism to provide high selectivity and optical dynamic range for detecting H2O2 in aqueous solution over similar reactive oxygen species (ROS) including superoxide, nitric oxide, tert-butyl hydroperoxide, and hydroxyl radical; Peroxyresorufin-1 (PR1), Peroxyfluor-1 (PF1), and Peroxyxanthone-1 (PX1) are first-generation probes that respond to H2O2 by an increase in red, green, and blue fluorescence, respectively. The boronate dyes are cell-permeable and can detect micromolar changes in H2O2 concentrations in living cells, including hippocampal neurons, using confocal and two-photon microscopy. The unique combination of ROS selectivity, membrane permeability, and a range of available excitation / emission colors establishes the potential value of PR1, PF1, PX1, and related probes for interrogating the physiology and pathology of cellular H2O2.

The invention discloses an in-vitro separation culture method for hippocampal neurons of an adult rat. The in-vitro separation culture method comprises the following steps of coating a culture plate; separating a rat brain; separating a hippocampus; preparing a cell suspension; further purifying the neurons; carrying out cell inoculation; and carrying out in-vitro primary culture. The invention aims at providing a high-purity and high-activity acquiring (separating and purifying) method for hippocampal neurons and an establishing method for a flow culture system suitable for long-term in-vitro culture of hippocampal neurons of the adult rat.

The invention belongs to the field of cell biology and relates to a method and reagent for the separation and culture of neurons. The method and the reagent are used for solving problems in the prior art, the current in-vitro hippocampal neuron separation and culture methods are improved, and the problem that the proliferation and activity of hippocampal neurons during primary culture can not be maintained is solved. According to the method and the reagent, a relatively mature in-vitro hippocampal neuron culture method is established, the number of obtained nerve cells is sufficient, the growth status is relatively good, and a large number of high-activity hippocampal nerve cells can be separated from mammalian hippocampal tissue, so that the requirements for the primary culture of the hippocampal cells are met, and the demands on experiments of cell biology during neuroscience research can be met.

The invention discloses an experiment method for studying the effect of tenuigenin on synaptic transmission of rat hippocampal neuron. The method adopts a micro control instrument, a microscopic, and a recording electrode to monitor the spontaneous current state of neuron in tenuigenin with different concentrations, and then the monitor current data is analyzed so as to obtain the influence mechanism and effect of tenuigenin on rat hippocampal neuron. The method adopts a whole-cell patch-clamp technology to research the effect of tenuigenin on spontaneous postsynaptic current in the rat brain hippocampal CA1 area so as to disclose the regulating effect and mechanism of tenuigenin on neuron synaptic transmission, thus the requirements of scientific research and drug development are fulfilled, and at the same time a basis is provided for the further research on the signal transmission mechanism of neuron. The method can be widely used in the pharmaceutical theory researches on neuron, the experiment method and equipment design are scientific and reasonable, and requirements of scientific research can be met.

The present invention describes a dissociated cell culture system comprising cells of the hippocampus, one of the brain areas affected by Alzheimer's Disease (AD) or amyloid beta-related diseases. This culture system comprises hippocampal neuronal and glial cells from animal models of AD, particularly, but not limited to, double transgenic mice expressing both the human APP mutation (K670N:M671L) (mAPP), and the human PS1 mutation (M146L) (mPS1), and serves as a powerful tool for the screening and testing of compounds and substances, e.g., drugs, for their ability to affect, treat, or prevent AD or β-amyloid-related diseases. The effects of a test substance on the cells in this culture system can be quantitatively assessed to determine if the test substance affects the cells biochemically and / or electrophysiologically, and / or optically, and / or immunocytochemically. The present in vitro culture system is advantageous for AD drug screening, because it is rapid and efficient. By contrast, even in the fastest animal model of AD, pathology does not start before the end of the second month. If such in vivo animal models are used, it is necessary to wait at least the two month time duration or longer to test for drug efficacy for AD treatment or prevention. At the same time the present invention provides a tool for production of amyloid-beta that can be used for electrophysiological, behavioral, and toxicological studies.

The invention discloses application of Opuntia ficus-indica Milpa Alta polysaccharides to the prevention and treatment of chronic neurodegenerative disease. The Opuntia ficus-indica Milpa Alta polysaccharides can improve the neurobehavioral disorder of rats, reduce the volume and cortices of cerebral infarction and the loss of hippocampal neurons, can reduce reactive oxygen species (ROS) in cells, has a good neuroprotection effect, can be taken as a medicine for preventing and treating chronic neurodegenerative disease of a central nervous system, and is used for preventing and treating chronic neurodegenerative disease of the central nervous system. The invention also provides an optimized process for extracting the Opuntia ficus-indica Milpa Alta polysaccharides. The extraction process has the characteristics of high polysaccharide yield and the like, is convenient, simple, economical and applicable, and is suitable for large-batch extraction.

The invention belongs to the field of cell biology and relates to a hippocampal neuron separation and primary culture method and reagent. The existing hippocampal neuron separation and primary culture method is improved aiming at the problems of the prior art, and the problem that the proliferation property and the activity of primary culture of the hippocampal neuron cannot be kept is solved. A relatively mature method for culturing hippocampal neurons in vitro is established, the obtained nerve cells are sufficient, the growth condition is good, a number of hippocampal neurons with high activity can be separated from hippocampus of mammals, the requirement of primary hippocampus cell culture is met, and the requirement of cell biology experiments in neuroscience research is met.

The invention discloses applications of opuntia ficus-indica milpa alta polysaccharides in improving rat neurobehavioral impairment, reducing cerebral infarction volume and hippocampal neuron loss and reducing ros (reactive oxygen species) generation in cells. The opuntia ficus-indica milpa alta polysaccharides have a good nerve protection effect, can be used as a medicament for preventing and treating central nervous system chronic neurodegenerative diseases. The invention also provides an optimized extraction process of the opuntia ficus-indica milpa alta polysaccharides. The extraction process has the characteristics of convenience, simplicity, economical efficiency, feasibility, high yield of polysaccharides, suitability for large-scale extraction and the like.

The invention discloses a method for culturing primary hippocampal neurons, wherein the method comprises the following steps: (1) digesting a hippocampal tissue; (2) blowing and dispersing the digested hippocampal tissue so as to obtain a cell suspension; (3) inoculating the cell suspension and culturing the hippocampal tissue for the first time for 2-4h so as to promote cell attachment; (4) changing a first culture solution inside a culture dish with a second culture solution, and culturing for the second time for 24h; (5) adding 1-2ug / ml of cytarabine to the culture dish and culturing for the third time for 24h; and (6) changing half volume of solution inside the culture dish by virtue of the second culture solution, culturing for the fourth time for at least 3 days and meanwhile changing half volume of solution twice every week so as to obtain the primary hippocampal neurons. By virtue of the method, primary hippocampal neurons can be rapidly and efficiently prepared and acquired; and the acquired primary hippocampal neurons are quite suitable to act as a cell model in a patch clamp experiment.

The invention belongs to the technical field of biotechnology and especially relates to a method for establishing a cell model for diabetes complicated with depression. The method comprises the following steps: adding glucose with a final concentration of 50 to 200 mmol / L and corticosterone with a concentration of 100 to 400 [mu]mol / L into a medium for culturing hippocampal neurons and carrying out intervention culture on the hippocampal neurons for 18 to 24 h so as to obtain the cell model. The cell model is evaluated through general morphological observation, HE dyeing, glucose consumption detection, intracellular neurotransmitter detection, MTT-process detection, flow cytometry, Hoechst fluorescent staining and TUNEL staining; and evaluation results show that the cell model has good effect and can be used for pathological and physiological research on diabetes complicated with depression, research on pathogenesis, in-vitro drug screening or drug evaluation.

The invention relates to the technical field of cell biology, in particular to a separation, purification and culture method of naked mole rat hippocampal neurons. The hippocampal neurons are separated from newly-born naked mole rat cerebral cortices and purified and cultured by the aid of multiple culture media comprehensively, and a reasonable culture method suitable for poikilothermal rodent mammal naked mole rat hippocampal neurons is found out. With the adoption of the method, a large number of naked mole rat hippocampal neurons with normal functional activity can be acquired conveniently, efficiently and economically, the cells can still keep biological characteristic in the in-vitro state in the in-vitro environment through culture under the low-oxygen condition, so that special physiological functions of the naked mole rat hippocampal neurons are further researched in pure in-vitro cell culture models directly and conveniently, and the important theoretical basis is provided for exploration of the biological mechanism and application to related clinical fields.

The invention discloses application of gentiopicroside (Gent) used as a protection medicine for preventing and treating epilepsy and epileptic cerebral injury. The application has the advantages that as shown by experimental results, the seizure attack latent periods of mice can be obviously prolonged when the gentiopicroside is used in safe dose ranges, the death rate can be reduced, and injury and death of hippocampal neurons of the mice after the mice are subjected to epileptic injury can be recovered; certain dose-efficacy correlation is shown and states that effects of delaying epileptic seizure, improving epileptiform activities and repairing injured cerebral nerves can be realized by the gentiopicroside.

The invention discloses an application of aphanalide I in preparation of neuroprotective medicines, and belongs to the field of medicines. An in vitro test proves that the aphanalide I has a significant effect of resisting Abeta25-35-induced apoptosis on hippocampal neuron under the damage condition; the effect is similar to that of a positive medicine ginkgo leaf extract EGb761; and the aphanalide I can be further researched and developed to prepare the neuroprotective medicines.

The invention provides an application of terazosin in a medicine for treating radioactive cognitive dysfunction. The terazosin is a pharmaceutical salt formed with terazosin as an active ingredient ora solvate thereof. The invention has the beneficial effects that whether the terazosin component can improve cognitive function damage and hippocampal neuron damage caused by whole brain irradiationis observed through experiments, and the terazosin component has certain neuroprotective effect in the occurrence and development process of radiation cognitive function damage.

Provided is a culture medium for the primary culture of hippocampus neurons of a neonatal rat. The culture medium is formed by adding 1,6-diphosphate fructose, fructose and nutritional supplements to a basic medium Neurobasal. Also provided is a method for the primary culture of hippocampus neurons of a rat using the abovementioned culture medium, comprising the steps of: (1) preparing the culture medium of hippocampus neurons; (2) sampling and digesting; (3) preparing a single cell suspension; (4) inoculating and culturing. Hippocampus neurons with good growing status and higher purity can be obtained using the abovementioned culture medium.

The invention discloses a medicinal and edible dual-purpose composition for preventing and treating vascular dementia and a preparation method thereof, belonging to the fields of food therapy and preparation of health products and traditional Chinese medicines. An optimal dosage form of a composition product is a soft capsule dosage form, and the medicinal and edible dual-purpose composition comprises the following components in parts by weight: 10-18 parts of a seabuckthorn seed procyanidine extract, 4-11 parts of a seabuckthorn flavone extract, 8-18 parts of a longstamen onion bulb extract and 63-72 parts of seabuckthorn seed oil. A preparation method of a soft capsule comprises the following steps: firstly preparing inclusions in the capsule into oil powder suspensions, and then preparing the oil powder suspensions into the soft capsule dosage form. The components of the medicinal and edible dual-purpose composition are all medicinal and edible dual-purpose varieties, are safe, and can be taken for a long time. The medicinal and edible dual-purpose composition disclosed by the invention can be used for effectively reducing the platelet aggregation rate and blood viscosity and increasing the cerebral blood flow, has extremely strong free radical removal and anti-oxidation effects, can be used for reducing the generation rate of MDA, repairing and protecting cranial nerve injury, and inhibiting hippocampal neuronal apoptosis, and plays roles in effectively preventing vascular dementia and treating vascular dementia.

The invention discloses an anti-aging black ginger extract and a preparation method thereof. The preparation method of the anti-aging black ginger extract comprises the steps that: an effective part of a black ginger is used as a raw material, the effective part of the black ginger is subjected to liquid nitrogen refrigeration, and then the effective part of the black ginger is crushed into powderto obtain black ginger powder; then the black ginger powder is used for extracting to obtain the anti-aging black ginger extract. The anti-aging black ginger extract obtained by the invention is a well-loved long-lived food material, can prevent the production of glycosylation products by inhibiting protein non-enzymatic saccharification reaction, thereby reducing the incidence of age-related chronic diseases and protein aging. In addition, the anti-aging black ginger extract also has a significant improvement effect on learning and memory disorders, enhances brain free radical scavenging ability, and improves the degeneration and shedding of hippocampal neurons.

The invention discloses a novel limonoid as well as a preparation method and medical application thereof, and belongs to the technical field of medicines. The novel limonoid is reported for the first time, is novel in structure and can be obtained by extraction, separation and purification of dry fructus aurantii immaturus. In-vitro tests show that the novel limonoid has a neuroprotection function on A beta(25-35)-induced hippocampal neuron injuries, also has the function of relieving A beta(25-35)-induced hippocampal neuron toxic injuries and can be used for developing neuroprotection medicines.

The invention belongs to the field of cell biology and relates to a method and reagent for the separation and culture of neurons. The method and the reagent are used for solving problems in the prior art, the current in-vitro hippocampal neuron separation and culture methods are improved, and the problem that the proliferation and activity of cortex nerve cells during primary culture can not be maintained is solved. According to the method and the reagent, a relatively mature in-vitro cortex nerve cell culture method is established, the number of obtained nerve cells is sufficient, the growth status is relatively good, and a large number of high-activity cortex nerve cells can be separated from mammalian hippocampal tissue, so that the requirements for the primary culture of the cortex nerve cells are met, and the demands on experiments of cell biology during neuroscience research can be met.

The invention relates to application of lactobacillus plantarum DP189 in preparation of medicine for preventing and / or treating Alzheimer's disease, and belongs to the field of biology, the lactobacillus plantarum DP189 is deposited in China Center for Type Culture Collection on March 25, 2019, and has a deposit number of CCTCC NO: M2019199. The lactobacillus plantarum DP189 is prepared into bacterial suspension with number of viable bacteria greater than or equal to 1 * 10 < 9 > CFU / ml. The lactobacillus plantarum DP189 can improve learning and memory ability, improve cognitive impairment, relieve damage degree of hippocampal neurons, reduce a level of malonaldehyde in serum, improve a level of superoxide dismutase in serum and improve levels of glutathione and glutathione peroxidase in serum; reduces levels of interleukin-1beta and tumor necrosis factor-alpha in serum, increases a level of interleukin-6 in serum, increases a level of dopamine in serum, and increases a level of 5-hydroxytryptamine in serum.

The invention discloses applications of L-serine or medicinal salts thereof in preparing medicaments used for preventing and treating brain injuries and nerve tissue injuries. The research finds that the L-serine is capable of significantly improving the neural functional grade of a mouse with traumatic brain injuries, reducing brain tissue loss after being injured, alleviating hippocampal neuron neuronal degeneration and necrosis significantly, and improving learning and memory functions. The applications of the L-serine as a nerve protection medicine in treating acute injuries caused by the brain injuries are disclosed for the first time.

The invention discloses the use of the G-CSF dimer in the preparation of a medicament for the treatment of neurodegenerative diseases. Use of the G-CSF dimer of the present invention can significantly increase the number of dopaminergic neuron in the substantia nigra in PD model animals and enhance the function of dopaminergic neurons. In addition, the G-CSF dimer can significantly reduce apoptosis of neuron in hippocampus and improve learning and memory ability of AD model rats. Serum half-life of the G-CSF dimer of the invention is prolonged and the loss of neurons is effectively prevented, providing a better therapeutic effect in treatment of neurodegenerative disease.

The invention discloses a traditional Chinese medicine composition and a preparation method and application thereof. The traditional Chinese medicine composition is prepared from rhizoma coptidis, cortex phellodendri, fructus gardeniae, radix salviae miltiorrhizae, turmeric root and rhizoma acori tatarinowii. The traditional Chinese medicine composition can significantly relieve the learning and memory dysfunction of 5 multiplied by FAD transgenic mice, reduce the expression of Abeta40 / 42 in hippocampus, increase the expression levels of presynaptic and postsynaptic protein in the hippocampusof 5 multiplied by FAD transgenic mice, relieve excitatory synaptic transmission disorder in the hippocampus of 5 multiplied by FAD mice, and meanwhile inhibit apoptosis of hippocampal neurons and proliferation of microglia in 5 multiplied by FAD mice; and in addition, the traditional Chinese medicine composition can inhibit expression of serum and hippocampal inflammatory factors in 5 multipliedby FAD mice, and therefore, a reliable theoretical basis is provided for the preparation of medicines from the traditional Chinese medicine composition for treatment or adjuvant treatment of Alzheimer's disease or preparation of health care products related to Alzheimer's disease from the traditional Chinese medicine composition.

The invention discloses an application of Matrine in preparation of medicine for treating cerebral arterial thrombosis. The experiment result shows that when the Matrine is used within a safe dose range and the dosage is 30 mg / kg bodyweight, certain dosage-efficacy correlation is shown, the volume of cerebral infarction in an ischemia area and the hippocampal neuron apoptosis rate can be reduced, autonomic dysfunction of the focal cerebral ischemia model of a mouse is remarkably relieved, the pathological injury of brain tissue is relieved, the activity of antioxidant enzyme in the brain tissue is improved, and the content of malonaldehyde is reduced. The Matrine has the functions of controlling incidence of cerebral arterial thrombosis, reducing disability and death caused by brain stroke and promoting neural functional recovery.