16 results about "Memory Dysfunction" patented technology

The invention discloses construction and application of a Primrose syndrome non-human animal model, and belongs to the technical field of animal models and disease research. According to the invention, through a CRISPR / Cas9 technology, a codon CAC for coding 596th histidine in a No.14 exon of a mouse Zbtb20 gene is subjected to site-directed mutagenesis into CGC for coding arginine, and a hybrid mouse model for simulating pathogenic point mutation (p.H596R) of human Primrose syndrome is constructed. The model can stably reproduce key clinical phenotypes of the Primrose syndrome, including overgrowth after adult, serum IGF-1 rise, memory dysfunction and anxiety behaviors, and shows abnormal hippocampal neuronal development and synaptic transfer related pathways and the like. The invention provides an important experimental tool for deeply revealing the pathological mechanism of Primrose syndrome, developing drug screening and treatment intervention research and the like.

The application discloses application of a Fructus Mume pill in preparation of a medicine for treating diabetic encephalopathy, and finds that there is a clear correlation between the Fructus Mume pill and the diabetic encephalopathy. Research results show that the Fructus Mume pill can improve the abnormal metabolic state of a diabetic model mouse, reduce learning and memory dysfunction and cognitive impairment under a diabetic state, and has a good prevention and treatment effect on the diabetic encephalopathy. The application clearly defines the pharmacological effect of the Fructus Mume pill in preventing and treating the diabetic encephalopathy and a possible action mechanism, and provides an experimental basis for TCM intervention on the diabetic encephalopathy. The application provides a new train of thought for research and development of a medicine related to the diabetic encephalopathy, and has a good application prospect.

The application discloses application of TESC as an Alzheimer's disease prevention and treatment target and relates to the field of biological medicines. A TESC overexpression (TESC-OE) hippocampal neuron cell line is constructed through lentivirus transfection, and the result shows that TESC-OE has the ability to resist A beta-induced neuron apoptosis and synapse damage. A TESC overexpression model in the hippocampus of a mouse is constructed through stereotactic injection of an adeno-associated virus, and the result shows that the hippocampal gray matter volume in the brain of the TESC-OE mouse is significantly larger than that of a wild type mouse. A beta stereotactic modeling is performed on the basis of TESC overexpression in the hippocampus, and the result shows that the TESC-OE mouse can significantly resist A beta-induced hippocampal atrophy, learning and memory dysfunction and impaired synaptic plasticity. Immunoblotting analysis reveals the molecular mechanism that TESC can increase the expression of anti-apoptotic genes in the hippocampus and reduce the expression of pro-apoptotic genes to exert a neuroprotective effect.

The application discloses a polypeptide targeting MST1 and p53 combination and application thereof in treatment of Alzheimer's disease, and relates to the field of biological medicine. The polypeptide provided by the embodiment of the application can block the neurodegenerative process from the upstream by targeting the MST1 kinase, has not only a direct neuroprotective effect, but also can improve synaptic plasticity, and is expected to realize substantial intervention on the disease process, rather than being limited to relieving symptoms. As a polypeptide drug, the polypeptide has a small molecular weight, better potential tissue penetration, lower immunogenicity, better safety and tolerance. The polypeptide can more effectively intervene in the AD disease process, improve cognitive impairment and memory dysfunction of patients, and provides a new way for AD treatment.

This invention discloses a method for constructing a zebrafish nervous system inflammation model and its applications, belonging to the field of animal neuroinflammation model technology. The method provided by this invention can construct a zebrafish nervous system inflammation model and has the advantages of short modeling time, high success rate, and simplicity. The zebrafish nervous system inflammation model constructed by this invention can be used to develop a drug screening platform for the effects of neuroinflammation, neurodegenerative diseases, and memory impairment, screening for potentially effective drugs by observing behavioral changes in zebrafish. It can also utilize the genetic background of zebrafish to study the molecular mechanisms of memory impairment, providing a theoretical basis for future treatments. It has applications in medicine, drug development, and the screening of natural active ingredients.

The application discloses an auxiliary diagnosis device for cognitive memory dysfunction based on a memory curve and belongs to the technical field of memory cognition auxiliary diagnosis. The auxiliary diagnosis device comprises a data preprocessing unit, a brain network feature acquisition unit, an activity signal feature extraction unit of a specified brain region, a memory model generation unit based on a memory curve and a cognitive memory dysfunction detection unit. The brain network feature acquisition unit is used for constructing a corresponding brain network based on preprocessed magnetic resonance imaging data and performing brain network feature extraction, and the extracted activity signal features are combined to serve as the input of the cognitive memory dysfunction detection unit, so that the auxiliary diagnosis result is output based on a preset detection model. The auxiliary diagnosis combines the auxiliary diagnosis result and a brain network topology map to generate a corresponding memory model and visually output and display. The application can be used for improving the accuracy of cognitive memory dysfunction auxiliary diagnosis and realizing the visual display of the memory forgetting process of a detection object.

The application discloses application of a taxifolin in treatment of Alzheimer's disease, SCA intervention can significantly improve cognitive memory dysfunction and protect brain tissue structure integrity; SCA can reduce abnormal deposition of A beta in the brain, reduce the number and distribution range of A beta positive plaque and alleviate AD core pathological damage; SCA can improve central nervous system pathological damage and cognitive dysfunction by regulating intestinal microecology. The application has important scientific significance and clinical transformation value for developing a safe and efficient new method for intervention of Alzheimer's disease.

The invention discloses application of bifidobacterium longum in preparation of a product for relieving cognitive impairment caused by methylmalonemia, and belongs to the technical field of microorganisms. The Bifidobacterium longum CCFM752 is preserved in the Guangdong Province Microbial Culture Collection Center, the preservation number is GDMCC (China General Microbiological Culture Collection Center) No.61157, and the preservation date is August 21, 2020. The Bifidobacterium longum CCFM752 disclosed by the invention has the advantages that the Bifidobacterium longum CCFM752 can be used for preparing the biological feed additive; the effect of the bifidobacterium longum CCFM752 for relieving the cognitive impairment of the MMA disease is comprehensively evaluated through an MMA model rat, and the effect is specifically reflected as follows: (a) the cognitive and memory impairment is relieved; (b) relieving activation of small glial cells in the hippocampus; (c) reducing the malondialdehyde level in serum and / or tissue to a certain extent; and (d) improving the level of glutathione peroxidase in serum and / or tissues to a certain extent.

The invention discloses an application of lactobacillus plantarum in preparation of a product for relieving individual oxidative stress and cognitive impairment caused by methylmalonic acidmia, the lactobacillus plantarum is preserved in Guangdong Microbial Culture Collection Center, the preservation number is GDMCC No.61163, and the preservation date is August 21, 2020. The lactobacillus plantarum can be applied to preparation of a product for relieving individual oxidative stress and cognitive impairment caused by methylmalonic acidmia. The relieving effect of lactobacillus plantarum CCFM1149 intervention on oxidative damage and cognitive impairment caused by MMA diseases is comprehensively evaluated through MMA model rats, and the relieving effect is specifically reflected as follows: (a) cognitive and memory impairment is relieved; (b) relieving activation of small glial cells in the hippocampus; (c) reducing malondialdehyde levels in serum and / or tissue; and (d) increasing the level of glutathione peroxidase in serum and / or tissues.

A cognitive dysfunction diagnosis device (1) includes: an acquisition unit (41) that acquires a distribution map that shows a distribution of a viewpoint of a subject with respect to a diagnosis image; and a diagnosis unit (39) that diagnoses the diagnosis image that includes a first image and an evaluation image in chronological order, the first image has a first region that includes a memorization image that is visually highlighted and a plurality of second regions that include a general image that is not highlighted, the evaluation image has a third region and the plurality of second regions, the third region includes an evaluation image, the evaluation image is an image that is similar to the memorization image and is displayed without visual highlighting at the same position as the memorization image, the diagnosis unit (39) calculates a fixation rate of the third region in the distribution map with respect to the evaluation image, and in a case where the fixation rate is lower than a threshold value, it is diagnosed that there is a suspicion of memory impairment.

The disclosure discloses Lactobacillus sp. BB1 enhancing memory ability, a fermented food and application thereof, and belongs to the technical field of microorganisms. The Lactobacillus sp. BB1 disclosed by the disclosure can be used for preparing functional foods, health-care products and drugs that enhance the memory ability of a healthy individual and improve memory impairment. The disclosure further provides the fermented food. The fermented food is prepared by fermentation with Lactobacillus sp. BB1, comprises a solid food, a liquid food and a semisolid food, and has a wide application prospect.

The application belongs to the field of traditional Chinese medicines, and discloses a traditional Chinese medicine supramolecular nanoparticle, which is prepared from Uncaria as a raw medicinal material, is extracted by heating reflux with 68-72% ethanol, is filtered, and is dried after being dialyzed after being dispersed in pure water, centrifuged, resuspended, and centrifuged again. The traditional Chinese medicine supramolecular nanoparticle has uniform particle size, a narrow particle size distribution, and high stability. The nanoparticle has obvious anti-nerve oxidation and obvious nerve protection effects according to in-vitro cell activity detection. The nanoparticle has good in-vivo nerve protection effect, can improve memory impairment and cognitive impairment, and can prevent hippocampal neuron reduction. Compared with Uncaria effective parts, the effective administration dose of the traditional Chinese medicine supramolecular nanoparticle is obviously reduced. The application also discloses a use of the traditional Chinese medicine supramolecular nanoparticle in preparation of a medicine for treating nervous system diseases.

The present application relates to the technical field of traditional Chinese medicine. The present application provides a traditional Chinese medicine composition for preventing and / or treating Alzheimer's disease, which comprises red ginseng and prepared radix falcaliae multiflorae. The present application also provides a preparation method of the traditional Chinese medicine composition for preventing and / or treating Alzheimer's disease. The traditional Chinese medicine composition has a protective effect on HT22 cells induced by A beta 1-42, can improve the anti-aging effect of HT22 cells, can significantly improve the learning and memory impairment of dementia model mice, can prolong the lifespan of N2 wild type Caenorhabditis elegans, can significantly inhibit the production and deposition of beta-amyloid protein closely related to Alzheimer's disease, can significantly activate the expression of hippocampus and cortex SIRT1, can significantly inhibit the expression of hippocampus and cortex aging marker genes of mice, can inhibit brain aging, and thus can effectively prevent and / or treat Alzheimer's disease.

This invention provides the application of metformin in the preparation of a drug for treating learning and memory deficits caused by schizophrenia with normal glucose and lipid metabolism, belonging to the field of pharmaceutical technology. This invention discovers that metformin, under conditions of normal glucose and lipid metabolism, can effectively reduce neuronal apoptosis and neuroinflammatory damage in the prefrontal cortex of the brain in schizophrenia patients, thereby improving their learning and memory deficits. This invention further provides that the molecular target of metformin in repairing prefrontal cortex damage in schizophrenia is CTSS, and that it can downregulate the expression level of CTSS in the prefrontal cortex. The metformin described in this invention represents a repurposing of an existing drug, shortening drug development time and costs, and providing an effective drug treatment for learning and memory deficits caused by schizophrenia with normal glucose and lipid metabolism.

The invention discloses application of a maravil and CCL5 neutralizing antibody to treatment of sepsis-related encephalopathy, and belongs to the field of medicinal chemistry. In-vivo experiments prove the novel application of the CCR5 antagonist malaviif and the CCL5 neutralizing antibody in treatment of sepsis-related encephalopathy (SAE), the survival rate of sepsis mice can be remarkably increased to 60% or above from about 30%, and anxiety-like behaviors and learning and memory dysfunctions of the mice can be effectively improved; according to the core mechanism, by inhibiting CCL5-CCR5 signal channels, pathological damage of hippocampal neurons is relieved, the level of key neuroinflammatory factors (IL-6, IL-1beta, CD68 and HMBG-1) is reduced, and by protecting dendritic spine density and synaptic related protein expression, abnormal synaptic phagocytosis of microglia is inhibited, so that comprehensive protection on the structures and functions of nerve synapses is achieved, and the application prospect is wide. And a verified brand-new target spot and an effective strategy are provided for clinical SAE treatment.

The invention discloses an application of a scopolamine model in an acetylcholin esterase inhibitor, and relates to the technical field of medicines.The application comprises the steps that S1, the learning memory of the scopolamine model is improved through a compound; s2, the inhibition effect of the compound on acetylcholin esterase is verified, and the compound is specifically (5S)-1-(3, 4-dihydroxyphenyl)-5-hydroxy-7-(4-hydroxy-3-methoxyphenyl) heptyl-3-ketone. A plurality of groups of experiments are designed to prove that the compound can remarkably improve the learning and memory dysfunction of AD model mice induced by scopolamine and has dose dependence, an enzyme activity inhibition experiment shows that the compound has strong inhibitory activity on AChE, the half inhibitory concentration (IC50) of the compound reaches 5.5 nM and is more remarkable than that of a clinical first-line anti-AD drug donepezil (IC50 = 12.2 nM), and the compound can be applied to preparation of anti-AD drugs. As the AChE activity increase is a key factor of cholinergic system injury in the AD pathological process, the compound is speculated to reduce hydrolysis of acetylcholine by inhibiting the AChE activity, and further improve the learning and memory ability of model animals.