109 results about "Neuronal protection" patented technology

The invention reveals that endogenous cholesterol metabolic product cholestane-3 beta, 5 alpha, 6 beta-triol (YC-5) has protection function for neuronal damage caused by cerebral ischemia, ischemia ofspinal cord, epileptic seizure and convulsion and relates to an application of YC-5 in preparation of neuronal protection medicine. The YC-5 can be prepared from cholesterol through two steps. The primitive cell culture in vitro and spinal cord ischemia animal model proves that YC-5 has protection function for neuronal damage caused by cerebral ischemia, ischemia of spinal cord, epileptic seizureand convulsion. YC-5 in effective dose has no toxic and side reactions. YC-5 is an effective neural protectant which aims to multiple molecular mechanism and cures cerebral ischemia damage, spinal cord ischemia damage, epileptic seizure and convulsion.

The invention provides compounds of Formula (I):

and pharmaceutically acceptable salts, solvates and stereoisomers thereof, wherein A, B, E, G, W, X, Y, Z, o, and R₁ are as disclosed herein (“Compound(s) of the Invention”), which are useful as cardio-protective and/or neuro-protective agents. The invention also provides pharmaceutical compositions comprising a Compound of the Invention and methods for treating, preventing and/or managing a vascular, cardiovascular or neurological disease or disorder, comprising administering to a patient in need thereof a Compound of the Invention.

Methods are disclosed for transducing neurons with heterologous genes using retrograde viral transport. The methods disclosed employ substantially non-toxic vectors, such as adeno-associated virus vectors, that are capable of retrograde axonal transport to introduce and express genes in the neurons. This method has applications in the mapping of neural pathways, in stimulating or inhibiting the growth of neurons, and in the treatment of various neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, and amyotrophic lateral sclerosis.

The invention relates to the field of traditional Chinese medicine extraction and separation, in particular to two indole new alkaloid compounds extracted, separated and identified from purslane, andan extraction and separation method and application thereof.The molecular formulas of the two new indole alkaloid compounds separated from purslane medicinal materials are respectively C17H13NO4 and C18H15NO5, and are respectively named as oleraindole A and oleraindole B.The invention also provides theextraction and separation method of the two new alkaloid compounds, which comprises the followingsteps of: sequentially adopting water decoction and extraction, silica gel column chromatography, polyamide column chromatography, ODS medium pressure column, and Sephadex LH-20 purification and liquid phase separation to preparethe two new alkaloid compounds. The structure of thetwo new alkaloid compounds is determined by ultraviolet, infrared, mass spectrum, hydrogen spectrum, carbon spectrum and two-dimensional nuclear magnetic spectrum analysis.The two indole new alkaloid compounds extracted from the purslane have anti-inflammatory, anti-tumor and nerve protection effects, and the salt orderivative of thetwo indole new alkaloid compounds can be used as a leading substance for synthesizing other compounds, as well as raw materials for new drug development and pharmacological activityresearch, and can be used for preparing anti-inflammatory, anti-tumor and nerve protection drugs.

The invention relates to the technical field of medicines, and relates to a steroid compound caulerpenone which is extracted and separated from C. racemesa and is novel in branch, a preparation method thereof and application to prepare nerve protective agents and medicines preventing Alzheimer's disease. In-vitro SH-SY5Y cellular-damage nerve protection effect experiment proves that the compound has obvious protective effect on SH-SY5Y cell with damaged beta-amyloid protein 25-35(Abeta-25-35) and is applicable to prepare nerve protective agents and medicines preventing Alzheimer's disease.

The invention provides a preparation method and application of a nerve growth factor controlled-release nano-carrier. A nerve growth factor and polylactic acid-hydroxyacetic acid copolymer are prepared into spherical nano-particles by an emulsion evaporation solvent method. A nerve growth factor nano controlled-release system packaged with the polylactic acid-hydroxyacetic acid copolymer is of a core-shell structure, wherein the core is the nerve growth factor, and the shell is uniform and compact polylactic acid-hydroxyacetic acid copolymer. According to the invention, proble that the existing nerve growth factor has a short body distribution half-life period and biological activity half-life period, can be metabolized quickly and degraded by enzyme easily and extremely unstable in an aqueous solution is solved. The method disclosed by the invention is simple to operat, and controllable in process. Moreover, secondary development on range of application of the nerve growth factor is carried out, the medicinal range of the nerve growth factor is expanded, and the never growth factor can be applied to preparation of a drug for treating the coronary heart disease with diabetes on the basis of the nerve protection effect of the nerve growth factor nano controlled-release system.

Disclosed are means of inducing neuroregeneration and/or neuroprotection in patients with damage to the nervous system. In one embodiment, Noble Gas containing compositions are administered to a patient suffering from a neurological injury, said therapy is administered alone, or in combination with other therapies useful in the induction of neuronal protection/stimulation of neurogenesis. In one specific embodiment, patients are treated with Noble Gas compositions to restore neural function subsequent to radiation therapy or chemotherapy for neoplasia of the brain. In another embodiment, Noble Gas compositions are administered prior to, concurrent with or after radiation and chemotherapy in order to induce a protective effect on non-malignant cells without substantially interfering with efficacy of radiation and chemotherapy.

The invention relates to a compound pharmaceutical composition containing midazolam and a neuro protective agent and particularly relates to a pharmaceutical composition. The compound medicament composition provided by the invention comprises an effective amount of midazolam or pharmaceutically acceptable salt thereof for treatment and/or prevention, the neuro protective agent and an optional andpharmaceutically acceptable carrier. The invention also relates to an application of a combination of midazolam or the pharmaceutically acceptable salt thereof and the neuro protective agent in the aspect of preparing medicaments for resisting anxiety, sedating and hypnotizing, resisting convulsion, resisting epilepsy and treating central muscular flaccidity and the like. The invention further relates to a method for preparing the pharmaceutical composition. By virtue of combining midazolam with the neuro protective agent such as vitamin B12, not only can the medical effect of midazolam be effectively exerted, but also cerebral nerve functions can be protected, and the pharmaceutical composition can be conveniently administrated and can take effect rapidly.

Provided are compounds, pharmaceutical compositions, cosmetic and dermatological compositions or nutritional supplement compositions, comprising omega-3 very-long-chain polyunsaturated fatty acids (n-3 VLC-PUFAs) and/or their endogenous hydroxylated derivatives thereof, known as elovanoids. This disclosure provides methods for neuroprotection, organ and tissue protection or restoration, prevention or slowing down of aging-related diseases and conditions, and sustainment of function during the aging process.

The invention relates to the field of traditional Chinese medicine extraction and separation, in particular to novel alkaloid compounds which are extracted, separated and identified from portulaca oleracea, and an extraction and separation method of the novel alkaloid compounds. The molecular formulae of the novel alkaloid compounds are C17H26N2O3 and C17H26N2O4 in sequence, and the novel alkaloid compounds are named as oleraciamide A and oleraciamide B. The invention further provides the extraction and separation method of the novel alkaloid compounds. The extraction and separation method comprises the following steps: performing water boiling extraction in sequence, performing ethyl acetate extraction, performing silica-gel column chromatography, performing ODS medium-pressure column and Sephadex LH-20 purification, and performing liquid-phase separation preparation, thereby successfully extracting and separating the two novel alkaloid compounds. The novel alkaloid compounds have the functions of inflammation resistance, tumor resistance and nerve protection, and the novel alkaloid compounds and salts or derivatives of the novel alkaloid compounds provided by the invention can be used as synthesis primers of other compounds, and raw materials for novel medicine development and pharmacological activity study, and can be used for preparing medicines or health products with functions of inflammation resistance, tumor resistance and nerve protection.

The invention discloses an effective part of a traditional Chinese medicine for treating Alzheimer disease and a preparation method and an application thereof. The effective part of the traditional Chinese medicine for treating Alzheimer disease is prepared by a method consisting of the following steps of: taking 6 parts of gambir plant, 4 parts of radix bupleuri, 6 parts of angelica sinensis, 6 parts of ligusticum wallichii, 8 parts of rhizoma atractylodis, 8 parts of poria cocos and 3 parts of liquorice; adding alcohol or water of which the weight is 5-15 times more than the total weight of all medicine materials, and extracting for 2-4 times by refluxing, wherein each extraction is performed for 1.5-4 hours; combining extracting solutions, concentrating to be without the smell of alcohol, and thus obtaining the concentrated solution; and putting the concentrated solution on a macroporous resin column, eluting in gradient by using ethyl alcohol solutions with the ethyl alcohol concentrations of 0-95%, collecting ethyl alcohol eluent with the concentration of 30%-50%, recovering solvent, and thus obtaining the effective part of the traditional Chinese medicine. According to the preparation method of the effective part of the traditional Chinese medicine for treating Alzheimer disease, a certain separating means is utilized to separate the effective part from liver restricting powder, and a modern pharmacological experiment method is used for proving that the neuroprotective effect of the effective part is obviously superior to that of the extract of the liver restricting powder, and thus the preparation method can be used for preparing medicines for resisting Alzheimer disease.

The invention discloses an indole N-glycoside compound, an extraction method, and an application in preparing drugs for preventing and treating nervous system diseases. The indole N-glycoside compoundhas a structure represented by a formula (I) in the specification. The indole N-glycoside compound of the invention can effectively protect PC12 cell damages caused by CoCl2, and the result suggeststhat the compound of the invention can be used as a neuroprotective drug.

The invention discloses an N-(4-benzylpiperidyl)-feruloylagmatine compound, a preparation method and application thereof. The compound has remarkable inhibition activity on self aggregation of butyryl cholinesterase and Abeta1-42, also has very strong antioxidant activity and has an obvious nerve protection function for hydrogen peroxide induced PC12 cell injury, thereby showing that the compound (I) is a multi-target inhibitor. Further in vivo experiments reflect that the compound has a favorable effect for treating the Alzheimer's disease, is low in toxicity and has good clinical application prospects.

The invention provides a cell model for screening drugs with neuroprotection activity, and application and a use method thereof. By using an NGF promoter as a target spot, the cell model established by the invention can be used for systematically, efficiently and extensively screening drugs with neuroprotection activity.

The invention relates to the field of biological pharmacy, and discloses a preparation method and application of a cyclosporine A-loaded efficient brain-targeted drug delivery system. The drug delivery system is composed of CsA and Fn; by utilizing the characteristic that ferritin is denatured in acetone and can be renatured in water, the CsA is wrapped in a cage structure (CsA(at)Fn) of the Fn through a solvent evaporation method; the CsA(at)Fn can be mediated by transferrin receptors on the surfaces of brain capillary endothelial cells to penetrate through a blood brain barrier and be accumulated in an ischemic area; on one hand, the CsA(at)Fn van protect the integrity of the blood brain barrier and reduce accumulation of inflammatory factors in a cerebral ischemic area; and on the other hand, the CsA(at)Fn taken by neuronal cells can inhibit the opening of mitochondrial permeability conversion pores of the neuronal cells, reduce the release of mitochondrial ROS and cytochrome C, inhibit the apoptosis of the neuronal cells and play a role in neuroprotection.

The invention specifically discloses a method for extracting, purifying and simultaneously preparing four high-purity ingredients of echinacoside, verbascoside, isoacteoside and tubuloside from herbacistanche and an application of the four single ingredients and compositions of the four single ingredients in different proportions in neuroprotection and bone protection. The method comprises stepsas follows: extraction of medicinal materials, alcohol precipitation with an extracting solution, macroporous resin purification and refining in combination with an HPLC-DAD/MS component automatic collecting system, that is, the medicinal materials of herba cistanche are extracted with a hot reflux method; preliminary purification is performed in an alcohol precipitation manner; the four ingredients are further purified with a macroporous resin purification method, and phenylethyl alcohol glycoside parts (with the content larger than or equal to 50% and meeting the requirements of five traditional Chinese medicine parts of novel medicines) of the required four ingredients are obtained; and finally, four ingredient monomers are efficiently prepared in a high-purity manner by use of the HPLC-DAD/MS component automatic collecting system and have the purity higher than 98%; and besides, the neuroprotection and bone protection functions of the four single ingredients and compositions of thefour single ingredients in different proportions is studied.