60 results about "MPTP" patented technology

Behavioral pharmacological data with the compound of formula (I), a novel and selective 5HT2A/2C receptor inverse agonist, demonstrate in vivo efficacy in models of psychosis and dyskinesias. This includes activity in reversing MK-801 induced locomotor behaviors, suggesting that this compound may be an efficacious anti-psychotic, and activity in an MPTP primate model of dyskinesias, suggesting efficacy as an anti-dyskinesia agent. These data support the hypothesis that 5HT2A/2C receptor inverse agonism may confer antipsychotic and anti-dyskinetic efficacy in humans, and indicate a use of the compound of formula (I) and related agents as novel therapeutics for Parkinson's Disease, related human neurodegenerative diseases, and psychosis.

The invention provides a metal-organic framework-superoxide dismutase assembly, a preparation method and an application thereof in preparation of drugs for treating Parkinson, and belongs to the technical field of biology. The framework makes use of biomimetic mineralization assembly strategy to immobilize SOD enzyme molecules into a metal-organic framework ZIF-8, coordination bonds are formed between SOD amino and zinc ions of ZIF-8, so that metal organic framework-SOD enzyme assembly (SOD@ZIF-8) are constructed based on enzymes. The temperature stability of SOD enzyme molecules and pH tolerance are improved through the frame work, by treating the SOD@ZIF-8 assembly as a therapeutic drug, at the cellular level, active oxygen produced by stimulation of MPP+ can be removed effectively, so as to relieve apoptosis caused by active oxygen; at the animal level, after intravenous chemotherapy by the assembly, the barrier in sports ability of Parkinson model mouse constructed by MPTP can be relieved, the expression level of tyrosine hydroxylase in substantia nigra is improved, the therapeutic effect is good.

This disclosure relates to a composition for prevention or treatment of neurodegenerative disease comprising longan arillus extract, or combined extract comprising longan arillus. The composition exhibits remarkably excellent prevention or treatment effect of neurodegenerative disease by containing longan arillus extract or combined extract comprising longan arillus, and particularly, it may effectively prevent or treat neurodegenerative disease by significantly protecting dopaminergic neurons from neurotoxicity due to MPTP selectively acting on dopaminergic nervous system and neurotoxicity due to aggregation of alpha-synuclein proteins.

The invention discloses an application of imatinib mesylate in preparation of drugs for resisting Parkinson's disease (PD). The application of imatinib mesylate (STI571) in preparation of drugs for treating neurodegenerative diseases belongs to the protective range of the invention, and the neurodegenerative diseases can be PD. An experiment proves that by continuous administration of the STI571, the dyskinesia induced by MPTP can be obviously improved, and the cognitive dysfunction of mice can be improved. The STI571 can obviously improve the dopaminergic neuron loss induced by the MPTP, can inhibit the rat CA1 and DG (diacylglycerol) region nerve cell apoptosis reaction induced by rotenone, can obviously inhibit the primary substantia nigra neuron apoptosis and Lewy protein expression induced by rotenone, and can inhibit the phosphorylation activation of PKC (Protein Kinase C) and Akt. The STI571 used as a specific inhibitor of c-Ab1 can resist the formation of PD, can improve the behavioral abnormity and cognitive dysfunction of PD, can be used as a new candidate drug for resisting neurodegenerative diseases such as PD and the like, and has favorable application prospects; and the clinical indications of the STI571 are greatly expanded.

The present invention provides an application of metformin in preparations of drugs for treatment of Parkinson's disease (PD). Experiment results show that: with the metformin, behavioral disorders of PD model mice are significantly improved; dopaminergic neuron loss of mice midbrain substantia nigra compact part (SNc) and ventral tegmental area (VTA) due to MPTP/p is reduced; levels of dopamine (DA) and metabolites thereof in corpus striatum are improved; excessive proliferation activation of astrocyte is inhibited; and reduction of dopaminergic neuron cell line SH-SY5Y cell viability, and increases of lactate dehydrogenase (LDH) generation and reactive oxygen species (ROS) generation are inhibited, wherein the reason of the reduction of dopaminergic neuron cell line SH-SY5Y cell viability, and the increases of lactate dehydrogenase (LDH) generation and reactive oxygen species (ROS) generation is MPP<+>. In addition, the results show that the metformin provides pharmacological effects for treatment of PD. According to the application of the present invention, the metformin is adopted as an active ingredient to prepare drugs for treatment of PD.

The invention provides a preparation method for an acute general Parkinson's disease monkey model. The preparation method comprises the following step of: continuously applying 0.36 mg/kg of MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) to a monkey for five times once every two days in an intravenous injection way. The invention provides a method for screening a medicament for treating the acute Parkinson's disease. By performing continuous peripheral vein injection of MPTP, an acute general Parkinson's disease monkey model with symptoms similar to those of an advanced-stage Parkinson's disease patient can be established. The model has stable symptoms within 1-4 months after establishment, and recovers rapidly later.

The invention relates to a substance content determination method and an application thereof. The substance is prepared from 225 parts of astragalus membranaceus, 100 parts of leech, 90 parts of ligusticum wallichii, 90 parts of angelica sinensis, 90 parts of safflower carthamus, 113 parts of peach kernel, 90 parts of radix paeoniae rubra, 90 parts of elecampane, 90 parts of rhizoma acori graminei, 60 parts of lumbricus, 90 parts of parasitic loranthus and 35 parts of acanthopanax extract. The content detection method has characteristics of simple and rapid operation and good repeatability andaccuracy. The method is advantaged in that pharmacological experiments of the substance show that the substance can significantly improve the mouse behavior coordination ability, significantly shorten the mouse pole climbing time, enhance the mouse grabbing force and the mouse swimming time, and significantly relieve MPTP-induced neuron damage, the substance content determination method can effectively control the inherent quality of the drug, and the new treatment application of the substance enlarges the clinical medication disease treatment range of the drug.

The invention belongs to the field of drugs or health products and particularly relates to an application of NADPH in positional drug-induced mitochondrial toxicity. The research finds that the NADPH has a protective effect on mitochondria damage caused by MPTP and rotenone, plays a neuroprotective effect through the protective effect, but has no protective effect on CCCP-caused mitochondria damage, so that the NADPH can be applied to control of neurodegenerative diseases, such as ageing, sub-health and huntington's chorea caused by mitochondrial dysfunction, vascular dementia, Lewy body dementia with frontal dementia, amyotrophic lateral sclerosis and multiple sclerosis.

The invention provides a preparation method for a hemi-parkinsonism monkey model. The method comprises the following steps of: applying 20 ml of MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) of which the dosage is 0.45-0.9 mg/kg to a monkey at the uniform speed 3ml/min. The invention further provides application of an animal model to screening of a medicament for treating the hemi-parkinsonism. The invention further provides a method for screening a medicament for treating the hemi-parkinsonism. The hemi-parkinsonism monkey model established with the method has the advantages of remarkable and typical symptoms and high stability after modeling; and an experimental animal survives easily, and is easy to breed. The stable time of the model is relatively long, and multiple stages of medicament treatment evaluation can be performed. Moreover, the model can undergo own control in the entire disease process, the using quantity of the model is saved, the requirement of the animal experiment ethnics is met, and the model is more economical and reasonable.

The invention belongs to the technical field of life science and particularly relates to a method for establishing a rhesus monkey model with a chronic process Parkinson's disease (PD). By using the interventional mode that MPTP is injected for a long time starting from a smallest dose which is gradually increased according to the difference in animal sensitivity mainly by means of intramuscular injection assisted with intravenous injection, a rhesus monkey is slowly stimulated to generate all clinical symptoms of the PD. The success rate of the animal model established through the method for establishing the rhesus monkey model with the chronic process PD is 100%, lewy bodies are observed in the made animal model, and the method is used for evaluating the effect of new PD drugs and screening the new PD drugs.

The invention relates to a compound with anti-parkinsonism pharmacological activity, which is called baicalin, with a systematic name that: the anti-PD pharmacological activity of baicalin acting on two kinds of animals with parkinsonism disease shows that the baicalin can protect the nigrostriatal dopaminergic neurons of animals of a PD model. The research results comprise that: (1) researches are carried out to check whether the baicalin can protect mice with parkinsonism disease induced by 1-methyl-4-phenyl1, 2, 3, 6-tetrahydropyridine (MPTP); and (2) researches are carried out to check whether the baicalin can protect rats with parkinsonism disease induced by rotenone; and the results of the researches shows that the baicalin can protect the nigrostriatal dopaminergic neurons of animals of the PD model, can reduce the loss of the nigrostriatal dopaminergic neurons, promote the growth of dopaminergic neuron protuberances, prevent the reduction of striatum dopamine, decrease the percentage of praxeological change occurring on animals, reduce total praxeological credits and improve the praxeological symptom of PD rats. The pharmacological activity of the baicalin suggests that the baicalin has great application significance in stopping the development of Parkinsonism disease.

The invention discloses an application of dioscin in preparation of a Parkinson's disease protection drug. MTT and morphological observation prove that the dioscin has an obvious protection effect onPC12 cells induced by 6-OHDA in vitro. Meanwhile, the dioscin has an obvious down-regulation effect on the remarkable increase of the expression level of GFAP and IBA-1 caused by MPTP induction of PDmouse, which indicates that the dioscin can inhibit the activation of microglial cells and astrocytes in brain tissues. According to clinical medication requirements, the dioscin can be used as a single effective component to be prepared into a medicine, and can also be combined with other medicines to be prepared into a compound preparation. The dioscin has the advantages of being safe, free of toxic or side effect, effective, economical, practical and the like.

The present invention relates to a composition for preventing, treating, or alleviating ischemia-reperfusion injury, containing bile acid. According to the present invention, bile acid increases intranuclear beta-catenin levels, interferes with the opening of a mitochondria permeable transition pore (mPTP), and has excellent effects, in ischemia-reperfusion injury animal models, of alleviating tissue injury and reducing the size of infarcts, thereby being usable in the prevention, treatment, or alleviation of ischemia-reperfusion injury.

The invention discloses a traditional Chinese medicine composition for treatment of Parkinson's disease, and belongs to the field of traditional Chinese medicine. The traditional Chinese medicine composition for treatment of Parkinson's disease is prepared from radix bupleuri, prepared rehmannia root, radix paeoniae alba and liquorice according to a certain weight part proportion. An MPTP induced mice subacute Parkinson's disease model proves that the traditional Chinese medicine composition has the effect of improving symptoms of mice Parkinson's disease.

The invention discloses a function application of SH2B adapter protein 1 in treating Parkinson's disease. Through neuron-specific over-expression of the SH2B1, dopaminergic neuron loss of substantia nigra pars compacta in an MPTP-induced mouse can be obviously improved, and the level of dopamine in striatum can be increased; and the proliferation and the activation of astrocytes and microglia of the substantia nigra pars compacta can be inhibited. Based upon the research results, it is indicated that the SH2B1 can be used for preparing medicines for treating the Parkinson's disease. Accordingto the invention, the therapeutic effect of the SH2B1 in the Parkinson's disease is verified for the first time; therefore, the SH2B1 is huge in market value and social benefit.

The invention discloses a traditional Chinese medicine composition for treating neurodegenerative diseases, which consists of the following traditional Chinese medicines in parts by weight: 2-60 parts of Smilax smilax, 1-30 parts of Gastrodia elata, and 1-30 parts of Angelica sinensis. The traditional Chinese medicine composition can obviously promote the proliferation of nerve cells and inhibit the death of nerve cells, and the combination of traditional Chinese medicines can play a synergistic effect compared with a single traditional Chinese medicine; it can be used in the Parkinson's disease model caused by MPTP in vitro. Reduce nerve cell death and restore the normality of mice, and be used in the Parkinson's disease model caused by rat 6-hydroxydopa, which can restore function and reduce brain necrosis area; the traditional Chinese medicine composition of the present invention can regenerate and replace dead Nerve area, to treat or prevent neurodegenerative diseases, reduce the activation of microglial cells in diseased tissue, and then protect nerve tissue, thereby reducing or alleviating, or even improving neuromotor function and improving a series of symptoms .

The invention provides application of isocorynoxeine to preparation of medicines with a neuroprotective effect. The medicines with the neuroprotective effect, provided by the invention, can effectively rescue the toxic effect of neurotoxin MPTP on dopaminergic neurons and have a protective effect on the dopaminergic neurons; compared with the common effects of lowering the blood pressure, expanding blood vessels and slowing the heart rate of the isocorynoxeine, the invention develops novel application of the isocorynoxeine, and a theoretical basis and an experimental basis are provided for further studying the neuropharmacology of a Chinese medicinal herb uncaria; furthermore, the isocorynoxeine provides a new idea and possibility for development of a Parkinson's disease treating medicine,an antidepressant medicine and an Alzheimer's disease treating medicine; especially, as the Parkinson's disease treating medicine, the isocorynoxeine has a particularly outstanding effect.

The invention belongs to the technical field of new application of plumbagin, and provides application of plumbagin in preparation of a medicine for preventing or treating Parkinson's disease. The toxic effect of neurotoxin MPP +/MPTP on Parkinson's disease and the toxic effect of plumbagin on antagonism of MPP <+>/MPTP on Parkinson's disease are observed through cell and animal experiments. Researches show that plumbagin can inhibit SH-SY5Y and PC12 nerve cells and dopaminergic neuron death and dyskinesia caused by neurotoxin MPP <+>/MPTP. Therefore, the plumbagin has good application prospects and huge new medicine development value in the aspects of Parkinson's disease prevention and treatment and neuroprotection.