13 results about "Pig model" patented technology

The application discloses a pig body size automatic measurement method, device and equipment, including: constructing a virtual pig model including a digital skeleton system and a measurement marker system, wherein the digital skeleton system includes a plurality of joint points representing key physiological structures of pigs, and preset measurement marker points in the measurement marker system are dynamically bound with joint points in the digital skeleton system; three-dimensional point cloud data of a target pig at different angles is acquired, and preprocessing including registration fusion and denoising is performed on the three-dimensional point cloud data to obtain target point cloud; the posture, size and position of the virtual pig model are adjusted through a preset optimization algorithm based on the target point cloud, and under the condition of meeting the physical constraint, the surface of the virtual pig model is fitted with the target point cloud to obtain a registration model; and spatial geometric calculation is performed according to three-dimensional coordinates of the registration model to obtain a body size parameter measurement result of the target pig.

The application discloses a pig disease diagnosis and treatment teaching training method, device and equipment based on AR, which comprises the following steps: loading a three-dimensional sick pig model corresponding to the current teaching scene through an AR device, and presenting the current symptom performance including visual elements and sound at the corresponding part of the three-dimensional sick pig model; receiving the diagnosis and treatment decision input by a user according to the current symptom performance; analyzing the diagnosis and treatment decision based on a preset pharmacology-pathology response model, updating the symptom state of the three-dimensional sick pig model according to the analysis result, and presenting the updated symptom state in real time as corresponding AR visual effects; when the user confirms the completion of the diagnosis and treatment operation based on the updated symptom state, comprehensively scoring the drug selection accuracy, dosage rationality, processing timeliness and diagnosis and treatment process integrity of the user in the diagnosis and treatment process, and generating a teaching evaluation report. The interactive training scheme can simulate a complete diagnosis and treatment process, support autonomous decision-making and dynamically feedback the disease outcome, and improves the teaching interactivity.

The application belongs to the field of biological medicine, and particularly relates to a Bama pig model for preclinical research of a weight loss drug COST and application. Healthy Bama pigs are first adaptively fed with ordinary feed, and then fed with high-fat feed to model, and the Bama pigs are detected for obesity-related indexes at a fixed time period every week; the high-fat feed comprises ordinary feed, beef tallow, artificial butter, sucrose, casein, experimental animal premix, microcrystalline cellulose, calcium bicarbonate, gypenoside, peppermint leaf, maslinic acid, ursolic acid, atractylodes, nutmeg. The application can quickly construct a Bama pig model with stable phenotype, and provides a reliable model for preclinical research of a new drug for treating obesity, and provides guarantee for research on the mechanism of obesity, screening, development and mechanism research of a new drug for treating obesity and the like.

The application provides a multifunctional nano-coated stent for preventing and treating in-stent restenosis and a preparation method and application thereof. Based on the multifunctional nano interface of EGCG-Cys, the liposome targeting VCAM-1 is used to deliver MnO2 nanoscale enzyme and FGF21 growth factor, so that the triple treatment effects of active oxygen scavenging, immune regulation and endothelial regeneration can be realized at the vascular lesion site. The EC-MFVL-WE43 stent creates a new paradigm for the design of interventional instruments, realizes the spatiotemporal precise release of drugs through the oxidative stress triggered degradation mechanism, and verifies the excellent anti-restenosis and anti-thrombosis performance in mouse, rabbit and pig models. Mechanism research shows that the nano-coated stent can reprogram macrophages to M2 repair type polarization, and effectively inhibit the abnormal proliferation of smooth muscle cells, providing a biological activity solution to break the vicious cycle of "inflammation-oxidation-restenosis".

The invention discloses an application of IL10 gene editing in an inflammatory bowel disease pig model. The gene editing is selected from gene knockout, gene knockdown, gene interference or gene point mutation capable of inactivating or reducing IL10 gene expression. It is proved for the first time that the IL10 <- / -> pigs obtained through gene editing can spontaneously suffer from enteritis in a conventional feeding environment, and a research tool closer to human physiological characteristics is provided; the IL10 < + / -> pig has no obvious inflammatory bowel disease phenotype in a conventional environment, the cost of model cultivation and propagation can be effectively reduced, a large animal model of the inflammatory bowel disease can be obtained through conditions induction and the like when needed, and different research requirements can be flexibly met; the IL10 < + / -> pig can be used as a model for researching IBD pathogenesis inducements and related mechanisms of people with low IL10 expression.

The invention provides a method for preparing an inflammation-enhanced atherosclerosis pig model. The method comprises the following steps: providing NLRP3R259W mutation and LDLR knockout double-gene modified pig cells; and regenerating the provided animal cells into a pig. The invention also provides a group of sgRNA sequences designed for editing the pig NLRP3 and / or LDLR gene loci. The inflammation-enhanced atherosclerosis pig model provided by the invention promotes research on correlation between atherosclerosis and inflammation.

The invention discloses a preparation method and application of a recombinant pig for expressing a human-derived HLA-B * 15: 02 gene. The invention provides a recombinant pig which is obtained by inserting a human HLA-B * 15: 02 gene into genome DNA (Deoxyribose Nucleic Acid) of the pig. The recombinant pig can be used as a pig model for screening drugs causing immune-mediated adverse reactions. The invention provides application of the recombinant pig in preparation of a pig model. The pig model is used for mechanism research of the immune-mediated adverse drug reaction or used for assisting establishment of a diagnostic standard of the immune-mediated adverse drug reaction or used for screening markers of the immune-mediated adverse drug reaction or used for research and development of drugs with the immune-mediated adverse drug reaction as indications. The invention has application and popularization values for related research and clinic of immune-mediated adverse reactions.

The invention provides a mutant STRA8 gene and a preparation method of an ovum occurrence retardation animal model. Compared with a wild type pig STRA8 gene, the pig STRA8 mutant gene has the insertion of c.403404insA and the deletion of c.441455del and c.516522del on one allele of a fifth exon, and has the deletion of c.404438del and c.521delT on the other allele of the fifth exon. The invention further provides a preparation method of the recombinant cell and the pig model with the ovum retardation. The STRA8 mutant gene provided by the invention can be used for preparing a female infertility animal model, so that a support is provided for researching a mechanism of female infertility, and meanwhile, the development of agriculture and animal husbandry is promoted.

The invention relates to the technical field of animal model construction, and particularly discloses a pregnancy intrauterine cochlea drug delivery animal model and a construction method and application thereof, and the construction method of the animal model provided by the invention comprises the following steps: establishing a pregnant mouse and Bama miniature pig model; under the guidance of an ultrasonic imaging system, in-vitro visual guidance is carried out on an intrauterine embryo cochlea injection path during pregnancy, an injection device is inserted into an embryo cochlea area, and drugs such as a gene carrier are injected into a target area through an ultrasonic guidance injection device, so that the intrauterine cochlea fixed-point administration animal model during pregnancy is obtained. According to the invention, the drug delivery of the embryo cochlea area is realized in a safer, faster and less-damage manner by combining the ultrasonic guidance technology. The animal model can be used for establishing drug therapy, gene knockout, supplementation and overexpression at the intrauterine embryo stage during pregnancy and simulating a technical scene for preclinical research of prenatal gene therapy of hereditary hearing loss.

The invention relates to the field of molecular biology, in particular to sgRNA for preparing an animal model of which a GJB2 gene is systematically knocked out. The technical problem to be solved by the invention is to provide a technology for establishing a systematic GJB2 gene knockout animal model for the field. According to the technical scheme for solving the technical problem, the sgRNA capable of preparing the animal model of which the GJB2 gene is systematically knocked out is provided. The sgRNA and Cas9mRNA disclosed by the invention are simultaneously introduced into a pig fertilized egg or an early development embryo through microinjection, so that an animal model of which the GJB2 gene is systematically knocked out can be prepared. The method can more accurately simulate a genetic mutation mode of GJB2 gene function deletion non-syndromic deafness with the highest morbidity in Chinese congenital deafness patient groups, and provides key support for researching a deafness causing mechanism of GJB2 gene deletion and researching and developing new technologies, new schemes or new equipment for treating and intervening GJB2 deletion deafness.

The invention provides a mutant STRA8 gene and a preparation method of a spermatogenesis arrest animal model. The mutated STRA8 gene and the spermatogenesis arrest animal model are both obtained by adopting gRNA (guide ribonucleic acid) for knocking out the pig STRA8 gene, and the gRNA comprises two different gRNA nucleotide fragments, namely gRNA1 and gRNA2. Compared with a wild type pig STRA8 gene, the mutant pig STRA8 gene has the deletion of 117bp on a No. 5 exon, in particular the deletion of c.404520del. The STRA8 mutant gene and the preparation method of the pig model with spermatogenesis arrest can be used for preparing a male sterility animal model, so that support is provided for researching the mechanism of male infertility, and meanwhile, the development of breeding and breeding technologies of agriculture and animal husbandry is promoted.

The present application relates to the field of animal models, in particular to a pulmonary vascular remodeling experimental pig model and a construction method thereof.The pulmonary vascular remodeling experimental pig model provided by the present application is obtained by injecting anhydrous ethanol into an experimental pig.Anhydrous ethanol is easily metabolized in vivo, which can avoid damage to other organs in the heart-lung circulation while inducing pulmonary hypertension, to some extent, eliminating the interference caused by the original lung injury caused by the injection of hydralazine.Secondly, through long-term induction of anhydrous ethanol, histological changes occur in the pulmonary artery, which not only helps to study the hemodynamics of pulmonary hypertension, but also further explores the mechanism of pulmonary hypertension from the organic changes of pulmonary artery remodeling.Finally, compared with small experimental animals, large experimental animals not only have better tolerance, but also provide convenience for related research of interventional medicine due to their larger vessel diameter.

The invention discloses a pig body size automatic measurement method, device and equipment, and the method comprises the steps: constructing a virtual pig model comprising a digital skeleton system and a measurement marking system, the digital skeleton system comprises a plurality of joint points representing the key physiological structure of a pig, and the measurement marking system comprises a plurality of joint points representing the key physiological structure of the pig; a preset measurement mark point in the measurement mark system is dynamically bound with a joint point in the digital skeleton system; obtaining three-dimensional point cloud data of a target pig only at different visual angles, and performing preprocessing including registration fusion and denoising on the three-dimensional point cloud data to obtain a target point cloud; taking the target point cloud as a reference, adjusting the posture, the size and the position of the virtual pig model through a preset optimization algorithm, and fitting the surface of the virtual pig model with the target point cloud under the condition of meeting the physical constraint to obtain a registration model; and carrying out space geometric calculation according to the three-dimensional coordinates of the registration model to obtain a body size parameter measurement result of the target pig.