2 results about "Hydralazine" patented technology

The application belongs to the fields of medicine and chemistry, and particularly relates to a preparation method of a dihydralazine impurity, S1: o-phenylenedinitrile is added into a reaction bottle, a solvent and a strong base are further added, and the reaction is stirred under heating and reflux; after the reaction is completed, the pH of the reaction system is adjusted by using an acid solvent, and then compound 2 is obtained through crystallization, filtration and drying, S2: compound 2 obtained in S1 and a solvent are added into a reaction bottle, hydrazine hydrate is added, after the reaction is completed, a part of the solvent is removed through concentration under reduced pressure, and compound 3 is obtained through crystallization, filtration and drying, S3: compound 3 obtained in S2 is added into a reaction bottle, a chloro reagent is added, and the reaction is completed under heating and reflux; after the solvent is removed through concentration, hydrazine hydrate is added. The method provides a preparation process of the dihydralazine impurity 1-amino-4-hydrazyl-phthalazine, and the process is simple, the reaction condition is mild, and the preparation can be completed by a laboratory.

The present application relates to the field of animal models, in particular to a pulmonary vascular remodeling experimental pig model and a construction method thereof.The pulmonary vascular remodeling experimental pig model provided by the present application is obtained by injecting anhydrous ethanol into an experimental pig.Anhydrous ethanol is easily metabolized in vivo, which can avoid damage to other organs in the heart-lung circulation while inducing pulmonary hypertension, to some extent, eliminating the interference caused by the original lung injury caused by the injection of hydralazine.Secondly, through long-term induction of anhydrous ethanol, histological changes occur in the pulmonary artery, which not only helps to study the hemodynamics of pulmonary hypertension, but also further explores the mechanism of pulmonary hypertension from the organic changes of pulmonary artery remodeling.Finally, compared with small experimental animals, large experimental animals not only have better tolerance, but also provide convenience for related research of interventional medicine due to their larger vessel diameter.