195 results about "Tetrandrine" patented technology

The invention belongs to the field of pharmaceutical manufacture, and relates to the structural modification of tetrandrine and application of multidrug resistance of reversal tumor cells, in particular to a 5-substituted tetrandrine compound and the application thereof in preparing anticarcinogen sensitizer. The invention discloses the 5-substituted tetrandrine compound, a pharmaceutically acceptable salt thereof, a preparation method thereof, a medicine combination, and the application of the above in an anticarcinogen sensitizer. In the compound, R is a substituted aryl group, a 5-element or 6-element heterocyclic ring, a cyano group, an amino group, an azide group, a carboxylic group and ester. The 5-substituted tetrandrine compound and the pharmaceutically acceptable salt thereof have favorable reversing activity to the multidrug resistance of tumor cells and can be used for preparing the anticarcinogen sensitizer.

The invention discloses novel application of tetrandrine in preparation of a drug for prevention, alleviation and/or treatment of depression. A result of a test shows that tetrandrine has obvious anti-depression curative effects, can produce effects fast and has few toxic and side effects, and thus is a safe, efficient and stable anti-depression drug of which a preparation process is simple. Therefore, tetrandrine is suitable for industrialized production and is easy to be popularized. The invention provides a novel drug source for prevention and treatment of depression and its concurrent diseases, disorder or illnesses.

The invention relates to a medicament composition containing tetrandrine, tetrandrine derivatives and a histone deacetylase (HDAC) inhibitor and application thereof in preparing medicaments for treating colon cancer, liver cancer, lung cancer, stomach cancer, encephaloma, sarcoma, pancreatic cancer, ovarian cancer, breast cancer, prostatic cancer or glioma. The medicament composition has remarkable synergistic effect, improves the curative effect of the medicament, decreases the administration dosage and reduces the occurrence of side effects.

The present invention belongs to the field of plant effective component extraction technology. In particular, it relates to a preparation method of hanfangchin A and hanfangchin B. Said method includes the following steps: (1), preparing material: pulverizing or slicing raw material; (2), extraction: adding quicklime into raw material, added amount is 6-10% of raw material weight, then using polar organic solvent to make thermal reflux extraction of extract, recovering solvent, making concentrate undergo the processes of standing still, crystallization and filtration so as to obtain precipitate; (3), crystallization: using acetone to dissolve precipitate with thermal reflux, concentrating the dissolved solution to proper extent, standing still and crystallizing; (4), crude product preparation: using cold benzene to dissolve crystal to obtain insoluble substance and benzene dissolved solution, said insoluble substance is hanfangchin B crude product, the concentrated paste to benzene dissolved solution is hanfangchin A crude product.

The present invention provides novel methods of preventing and treating prostate cancer with tetrandrine, a bisphehol-isoquinoline alkaloid isolated from the Chinese herb Stephania tetrandra. The present invention also provides the use of tetrandrine for the manufacture of a pharmaceutical composition for treating prostate cancer. The tetrandrine can be used as any isomer of tetrandrine such as the (R,R), (S,S), (R,S), and (S,R) isomers and any combination of these isomers. The present invention also provides methods of inhibiting the growth of prostate cancer cells in vitro by contacting the cells with tetrandrine. The present invention also provides methods of inducing apoptosis in prostate cancer cells in vitro by contacting the cells with tetrandrine.

The invention relates to an extraction method of tetrandrine and demethyltetrandrine. The extraction method comprises the steps of: uniformly mixing Stephania tetrandra powder and quick lime powder, carrying out alcohol extraction to obtain total alkaloid extract, adjusting the pH value to 2-4, extracting, and standing for layering; carrying out alkalization and reduction on the extract liquid to obtain extract, dissolving the extract in acetone, filtering, concentrating and crystallizing to obtain tetrandrine crude product, and recrystallizing the tetrandrine crude product to obtain a tetrandrine product; and adjusting pH value of the acidic aqueous solution obtained after standing to 9-11, extracting to obtain extract, dissolving the extract in acetone, filtering, concentrating and crystallizing to obtain demethyltetrandrine crude product, and recrystallizing the demethyltetrandrine crude product to obtain a demethyltetrandrine product. Compared with the prior art, the extraction method has the advantages that: the characteristics of the solubility difference between free tetrandrine and free demethyltetrandrine and demethyltetrandrine salts generated by reaction between acid and each of tetrandrine and demethyltetrandrine is utilized, tetrandrine and demethyltetrandrine can be separately obtained by adjusting the pH value; the operation controllability is good, the component loss is low, the extractant can be reused, benzene is not used in extraction process, and the production cost is low; the extraction method is suitable for industrial production; tetrandrine and demethyltetrandrine products can be simultaneously obtained; and the raw material utilization rate is effectively improved and the production cost is lowered.

The invention belongs to the chemistry of natural product field, which more particularly relates to a preparation technique of tetrandrine alkaloid. Dilute acid solution is used for percolating tetrandrine meal, leachate is absorbed by macroporous resin column, after being washed, alcohol solution with ammonia/amine is used for eluting, and then total alkaloids is get by concentration, finally tetrandrine alkaloid with high purity is obtained by crystallization. The tetrandrine alkaloid has the advantages of easy operation, less hazardous waste and residue, low cost, high yield, and getting all the tetrandrine alkaloid for once, etc. The method is a simple, economic, fast and strong practical processing method which comprehensively utilizes the tetrandrine alkaloid.

The present invention discloses an application of tetrandrine in hepatic stellate cell migration inhibition drug preparation. According to the present invention, a PDGF-induced human hepatic stellate cell line (LX-2) migration screening platform is designed, and results show that PDGF-induced hepatic stellate cell (HSC) migration can be inhibited with 10<-4>-10<-7> mol/L tetrandrine.

The invention relates to the technical field of medicines and particularly relates to a polyurethane foam dressing and a preparation method thereof. The polyurethane foam dressing is prepared from thefollowing raw materials: waterborne polyurethane prepolymers, water-soluble chitosan, tetrandrine, tamoxifen, isocyanate, a surfactant, a catalyst, a foaming agent and purified water. In the polyurethane foam dressing provided by the invention, the waterborne polyurethane prepolymers and the isocyanate are taken as the raw materials, the surfactant, the catalyst and the foaming agent are added, three active ingredients of the water-soluble chitosan, the tetrandrine and the tamoxifen are added, and all the ingredients have the synergistic interaction, so that the prepared polyurethane foam dressing has an obvious effect of wound healing, the wound healing speed is obviously improved, and the wound healing time is shortened to 5-6d.

The invention relates to a composite containing tetrandrine, and discloses a composite injection containing the tetrandrine and a preparation method thereof; the composite injection consists of the tetrandrine, latent solvent, stabilizing agent and sodium chloride; wherein, the content of the tetrandrine is 2-60%, the content of the latent solvent is 2-40%, the content of the stabilizing agent is 1-8%, and the content of the sodium chloride is 9-90%; the proportion among the tetrandrine, the latent solvent and the stabilizing agent is 1:0.5:0.01-1:1:1. The method is that: right amount of water used for injecting is added with 2-60% of latent solvent, 1-8% of stabilizing agent and 9-90% of sodium chloride for dissolving; the 2-60% of tetrandrine is added to be stirred and dissolved, and then added with water to full dose; after being mixed, the content and pH value of the tetrandrine is measured, and then poured into a bottle after being filtered; after sterilization, lamp check and quality control are carried out, the injection is prepared. The composite injection is easy to store, has little irritation and good stability, can overcome the problems of easy precipitation of the tetrandrine and clinical pain.

The invention discloses a tetrandrine liquid crystal nanoparticle preparation for eyes and a preparation method thereof. The method includes the steps of firstly, adding lipid material, stabilizer and tetrandrine to solvent absolute ethyl alcohol to be heated and dissolved, and conducting decompression rotary steaming to thoroughly remove ethyl alcohol to obtain a uniform oil phase; secondly, adding penetration enhancer and positive ion material to distilled water to be stirred and completely dissolved to obtain a water phase; thirdly, adding the water phase to the oil phase to be shorn, dispersed and cooled to the room temperature to obtain an initial emulsion; fourthly, conducting ultrasound treatment on the initial emulsion to obtain the tetrandrine liquid crystal nanoparticle preparation for eyes. The preparation has huge film surface area, strong medicine carrying capacity, good biological compatibility, high biological adhesiveness and high long-term stability, the number of dosage times can be decreased, and the dependence of patients on medicine is improved. The preparation has the effects on packaging and protecting medicine, increasing dissolvability, improving stability and promoting medicine absorption.

The invention belongs to the technical field of pharmacy and particularly relates to an erythrocyte membrane-encapsulated tetrandrine PLGA nanoparticle and a preparation method and application thereof. The nanoparticle structurally includes an erythrocyte membrane, PLGA and tetrandrine, the tetrandrine is combined with PLGA to form a tetrandrine-PLGA nanocore, and the erythrocyte membrane is encapsulated outside the tetrandrine-PLGA nanocore. The preparation method is simple and efficient. The nano preparation improves the biocompatibility of a whole drug delivery system, long circulation andsustained release of the tetrandrine in the human body are achieved, toxic and side effects caused by too high blood medicine peak concentration of common injections are avoided, and the nanoparticlehas a good application prospect.

The invention relates to the technical field of cardioplegic solutions, in particular to a cardioplegic solution containing lidocaine and adenosine and a preparation method thereof. Every 1,000ml of organ preservation solution comprises the following components by weight: 5.0-7.0g of sodium chloride, 2.0-3.0g of potassium chloride, 0.3-0.5g of magnesium sulfate, 0.05-0.1g of calcium chloride, 5-8g of glucose, 0.3-0.6g of lidocaine, 0.1-0.2g of adenosine, 0.01-0.02g of astragaloside, 0.05-0.08g of coenzyme Q10, 0.005-0.01g of tetrandrine, 0.005-0.01g of higenamine, 5-8g of histidine and the balance of double distilled water.

The invention discloses a hanfangchin a nanoemulsion injection and a preparation method thereof, containing the hanfangchin a, surfactant, cosurfactant, oil phase and water phase. The nanoemulsion of the invention has the advantages of simple preparation method, transparent appearance, 10-100nm of particle diameter and good stability, thereby remarkably improving solubility of the hanfangchin a, so as to improve bioavailability of drugs, and changing intracorporal process of drugs, so as to increase targeting to liver, reduce toxic and side effect and improve treatment effect.

The invention discloses a tetrandrine derivative with antitumor activity and a preparation method thereof. The structural formula of the compound is shown in the specification. The preparation method comprises the following steps: dissolving bisbenzylisoquinoline compound serving as raw material in certain solvent; adding an alkali reagent and a reactant Y, and mixing; reacting for 0.1 to 72 hours under temperature of -20 to 300 DEG C; neutralizing the reacted solution with acid, and then separating and purifying to prepare the objective product. The tetrandrine derivative prepared by the invention can well inhibit proliferation of human hepatoma cell line HepG2 and pancreatic cancer cell SW1990, and can be used for preparing antitumor medicines.

The invention discloses tetrandrine derivatives and a preparation method thereof. The structural general formula of the compounds is as shown in the specification. The method comprises the following steps: dissolving bisbenzylisoquinoline compounds used as raw materials in a certain solvent, adding a reactant Y, mixing, reacting for 0.1-72 hours at a temperature ranging from minus 20 to 300 DEG C and under a neutral or alkaline condition, adding an acid to a solution obtained after reacting and neutralizing, and then carrying out separation and purification to obtain target products. The tetrandrine derivatives prepared by the method have an outstanding inhibition effect on the proliferation of the human hepatocarcinoma cell line HepG2 and the human colon cancer cells HT29, and thus can be applied to preparing anti-tumor medicines.

The invention relates to bisbenzylisoquinoline quaternary ammonium salt as well as a preparation method and an application thereof in preparing an antitumor drug. A structure of the bisbenzylisoquinoline quaternary ammonium salt is shown in a general formula (I) described in the specification and a substituent group is shown in the specification. The bisbenzylisoquinoline quaternary ammonium salt disclosed by the invention is applied to preparation of an antitumor drug. The compound disclosed by the invention, which has a bisbenzylisoquinoline parent nucleus structure and has the characteristics of ester quaternary ammonium salt, has achieved a pancreatic cancer cell SW1990 proliferation inhibiting rate above 50% and a hepatoma cell line HepG2 inhibiting rate above 50% as well when the compound is 0.5ug/ml, and activity of the compound is much higher than that of an existing tetrandrine compound.

The invention relates to the field of cosmetics, in particular to multi-effect plant cleanser. The multi-effect plant cleanser is mainly prepared from ginkgo biloba extracts, tetrandrine extracts and second components. The second components are oat extracts or willow bark extracts. The multi-effect plant cleanser is prepared from, by weight, 2-6 parts of ginkgo biloba extracts, 1-3 parts of tetrandrine extracts and 2-6 parts of the second components. Through many times of experiments, it is found that the multi-effect plant cleanser is prepared from specific ginkgo biloba extracts, tetrandrine extracts and oat extracts or willow bark extracts, the components are used in cooperation, deep-layer dirt gathered in pores can be effectively decomposed, the effects of removing comedo and closed comedo, shrinking pores, softening and awakening skin, adjusting sebum, diminishing inflammation and relieving acne, enhancing skin immunity and the like are achieved, the skin is made soft, smooth, tender, white and clear, and use is safe.

The invention provides a preparation method of alkaloid in stephania tetrandra. The alkaloid contains cissamine extract and mixed extract of tetrandrine and hanfangichin. The preparation method comprises the following steps of: soaking stephania tetrandra dry powder with ammonia water; drying and then adding 75-90 percent low-carbon alcohol to reflux and extract; concentrating extract until no alcohol smell exists; adjusting the extract to be acidic with diluted hydrochloric acid; filtering off precipitate; adding alkali into the acid solution to precipitate; washing precipitate; (1) combining the washed solution and alkali liquor part, concentrating through a nanofiltration membrane and drying concentrated solution to obtain cissamine extract; and (2) adding acetone into the precipitate to dissolve, filtering off insoluble, adding water into acetone solution to settle out, filtering the precipitate and drying to obtain the mixed extract of tetrandrine and hanfangichin. Two alkaloid extracts are simultaneously prepared by using the preparation method; and the preparation method has the advantages of high raw material utilization rate, safe preparation process, low cost, short period and contribution to industrial large-scale production.

The invention discloses new application of tetrandrine in fungal bio-film resistance, and relates to application of tetrandrine in the field of fungal bio-film resisting treatment medicaments. The tetrandrine compound has the following chemical structural formula; and experimental results show that the tetrandrine compound has high fungal bio-film resisting activity in vitro. Experimental results show that the tetrandrine can effectively inhibit growth and proliferation of the fungal bio-films in vitro and effectively inhibit mature bio-films.

The invention relates to a method for preparing tetrandrine alkaloids by coupling in-situ conversion-adsorptive separation technology, and the preparation process comprises: 1) tetrandrine raw drug is ground and soaked into ethanol water solution for heat preservation and agitation. After the mixed solution is filtered, filtrate is processed by reduced pressure distillation, and ethanol is recovered; acetic acid water solution is added into the processed filtrate which is then filtered, ammonia water is used for adjusting the pH value of the filtrate to be within the range of 10-12, and precipitate is separated out; the obtained precipitate is dissolved into the acetic acid water solution and centrifugated, and supernate is collected and called as adsorption solution for short; 2) after the adsorption solution with the quantity being 3-5 times of bed volume is absorbed by resin column, a resin bed layer is washed by a great quantity of deionized water, and then the tetrandrine alkaloids extract is obtained by the steps such as using the ammonia water to pass through the resin column, etc. In the preparation process, noxious solvent is not used, and other solvents are consumed in a small quantity; meanwhile, resin is not needed to be processed by acid-alkali regeneration after the extraction period of every time, so that the operation technique is greatly simplified, the resin can be reused, a great deal of resources can be saved, the extraction efficiency is improved, and the method is suitable for industrialized large-scale production. The prepared tetrandrine alkaloids have the purity being higher than 70% and the yield being higher than 95%.

The invention relates to a method for extracting tetrandrine. The method comprises the following steps of: adding alkali into high-concentration ethanol for extracting, dissolving with an acid, separating with D732 macroporous cation exchange resin, extracting with toluene, and alternately crystallizing with acetone ethanol to obtain the product. The method of the invention for producing tetrandrine has the advantages of simple operation, low cost and high purity of the product.

The invention relates to an application of tetrandrine/dipotassium glycyrrhizinate in preparing an anti-allergy drug and belongs to the field of drug compositions. With the adoption of the application, as the tetrandrine is assisted and matched with the dipotassium glycyrrhizinate, the antiallergic efficacy can be obviously improved; and cosmetics and skin care products prepared by adopting the drug composition are good in the allergy resistance and are suitable for persons with a sensitive skin.

The invention relates to a method for separating and preparing tetrandrine and hanfangchin through ion exchange and column chromatography on silica gel. Fourstamen stephania root is dried root of a menispermaceae stephania plant stephania tetrandra, an effective ingredient of the fourstamen stephania root is sinomenine, main effective ingredients of sinomenine are tetrandrine and hanfangchin, and the sinomenine has anti-myocardial ischemia, anti-arrhythmia, blood pressure lowering, anti-inflammatory, anti-silicosis, anti-cancer and analgesic effects. When in drug use, the purity requirement of the tetrandrine and hanfangchin reaches more than 99%, and extraction and purification are important reasons limiting the drug use of the tetrandrine and hanfangchin. At present, a technology for extracting and purifying the tetrandrine and hanfangchin applies massive toxic and expensive chloroform and strong base, and the chloroform has great toxic effect on human and the environment and is also strong corrosive on equipment for enlarged production; besides, sample purity is not high, and a technological process is complex. At the current situation, the invention develops a method for obtaining high-purity tetrandrine and hanfangchin through rapid separation and purification by applying ion exchange resin and a C18 bonded silica gel column chromatography and also discloses a method for recrystallizing and purifying the tetrandrine and hanfangchin by utilizing ethyl alcohol. The method provided by the invention has the advantages that a preparation technology does not apply any toxic reagent, column chromatography filler can be recycled, and ethyl alcohol can be recycled.