30 results about "Trimethoprim" patented technology

The invention discloses a trimethoprim hapten as well as synthesis and application thereof. The structure of the trimethoprim hapten is as shown in a formula I. The trimethoprim hapten provided by the invention can fully expose the antigenic determinant of trimethoprim and maintain the spatial conformation of trimethoprim, has good immunogenicity, and is mild in synthesis conditions, few in reaction steps, high in yield and capable of reducing the cost of raw materials. The trimethoprim colloidal gold detection product provided by the invention has the characteristics of strong specificity and high sensitivity, can realize accurate and rapid detection of trimethoprim in a sample, and has no cross reaction with other drugs. And the formula I of the # imgabs0 # is shown in the specification.

The invention provides a preparation method of a compound sulfonamide medicinal preparation. The compound sulfonamide medicinal preparation contains a premix prepared from sulfadiazine, sulfamethoxazole, trimethoprim, sodium bicarbonate and a slow-release coating medium. Compared with sulfadiazine, sulfamethoxazole and trimethoprim preparations, the sodium bicarbonate coated compound is high in bioavailability, and after the sodium bicarbonate coated compound is added, caking in urine can be remarkably reduced, and the stability of the preparation is not affected.

This invention provides an anti-trimethoprim single-chain antibody for detecting trimethoprim-like drug residues, its preparation method, and its application. The amino acid sequences of the single-chain antibody are shown in SEQ ID3, SEQ ID4, and SEQ ID6. Compared with traditional monoclonal antibodies, the single-chain antibody has lower production costs, is simpler to prepare, and is easier to manipulate at the gene level. This invention utilizes trimethoprim-specific monoclonal hybridoma cells and employs phage display technology to screen for high-affinity single-chain antibodies. A single-chain antibody mutant with even higher affinity was successfully obtained through computer simulation combined with site-directed mutagenesis. Based on this mutant, an ic-ELISA was established for the detection of trimethoprim residues in animal-derived foods, meeting the needs of practical applications.

The present application belongs to the technical field of wastewater treatment, and particularly relates to a seawater breeding wastewater antibiotic removal method based on oyster shell composite filler. Fine gravel, oyster shell and functional mineral are compounded in a fixed volume ratio of 2:1:1, and are laid in a structure with a limited particle size range and homogeneity without stratification. A salinity-directed salt-tolerant domestication process and a tidal intermittent operation mode of 48h hydraulic retention + 24h emptying and reoxygenation are matched, and salt-tolerant functional bacterial flora of Proteobacteria, Paracoccus and Shigella are enriched. The present application can adapt to a wide range of salinity conditions of 10‰-30‰, relieve high salt inhibition and ion competition, simultaneously and efficiently remove three typical antibiotics of conventional pollutants, sulfamethoxazole, trimethoprim and enrofloxacin, and reduce resistance genes. The sulfamethoxazole removal rate can reach 93.94% under the condition of 30‰ high salinity, the system runs stably and has strong adaptability, and has certain engineering application value and large-scale popularization potential in the field of seawater breeding wastewater treatment.

The invention discloses a test strip for detecting fluoroquinolones, sulfonamides, tetracyclines and trimethoprim drug residues in aquatic products and a test method, and belongs to the technical field of drug detection.The preparation method comprises the steps that trimethoprim hapten is synthesized; synthesizing a quinolone hapten, a sulfanilamide hapten and a tetracycline hapten; preparing an immunogen and a coating antigen; preparing a monoclonal antibody; preparing a gold-labeled antibody; and pre-treating and assembling. According to the invention, an integrated detection scheme of four types of medicines is constructed, so that residual detection of fluoroquinolones, sulfonamides, tetracyclines and trimethoprim in aquatic products can be synchronously completed, and different detection tools or batch detection for single type of medicines is not needed. In an actual detection scene of aquatic products, repeated steps of sample treatment and detection operation can be greatly reduced, waste of aquatic product samples caused by repeated sampling is avoided, meanwhile, the overall detection period is shortened, and the rapid quality control requirement of the aquatic products from fishing / breeding to circulation links is met.

The invention discloses a series connection synthesis method of 3, 4, 5-trimethoxy benzaldehyde. The 3, 4, 5-trimethoxybenzaldehyde is an important medicine and fine chemical engineering intermediate, and can be used for synthesizing a sulfanilamide synergist trimethoprim and an anti-cancer drug etoposide. According to the invention, 3, 5-dibromo-4-hydroxybenzaldehyde is taken as a raw material, a sodium methoxide-methanol solution is taken as a methoxylation reagent, dimethyl carbonate is taken as a methylation reagent, and tandem synthesis of 3, 4, 5-trimethoxybenzaldehyde is realized by adding a plurality of catalysts under normal pressure. The method does not need to separate a reaction intermediate or use a high-pressure reaction container, and has the advantages of simplicity, convenience and safety in operation, low reagent toxicity, higher yield, short reaction time and the like.

The invention discloses an external antibacterial medicine composition as well as a preparation method and application thereof, and belongs to the technical field of antibacterial medicines. The external antibacterial pharmaceutical composition provided by the invention comprises a medicinal active component and a pharmaceutically acceptable carrier, wherein the medicinal active component comprises polymyxin B sulfate, trimethoprim sulfate and lidocaine; every 10 g of the antibacterial pharmaceutical composition for external use is prepared from the following components in parts by weight: 40000 to 60000 U of polymyxin B sulfate, 0.08 to 0.16 g of trimethoprim sulfate and 0.30 to 0.35 g of lidocaine. The pharmaceutical composition disclosed by the invention has broad-spectrum inhibition on multiple drug-resistant bacteria such as common gram-negative drug-resistant bacteria and acinetobacter baumannii on a wound surface, synchronously plays a synergistic role in relieving inflammatory response of the wound surface and relieving local pain, and remarkably improves the medication safety and the comprehensive treatment effect while ensuring excellent antibacterial activity.

This application relates to the field of biotechnology, and particularly to primer pairs, primer-probe compositions, PCR premixes, kits, and methods for detecting multiple respiratory pathogens. Nucleic acid sequences are shown in primer pairs as indicated by SEQ ID NO: 1-2, 4-5, 7-8, 10-11, 13-14, 16-17, 19-20, 22-23, 25-26, 28-29, 31-32, 34-35, 37-38, and 40-41. For target respiratory pathogens, this application designs specific amplification primer pairs, paired with suitable probes, enabling the simultaneous detection of eight targets. Furthermore, the detection process avoids interference from heme, trimethoprim, sulfamethoxazole, amphotericin B, itraconazole, fluconazole, azithromycin, adrenaline, and lidocaine hydrochloride in the test sample. It exhibits good anti-interference properties and shows no cross-reactivity with *Rhodococcus equi*, *Candida tropicalis*, and *Candida krusei*.

The present disclosure provides a system for treating melioidosis, comprising a first antimicrobial agent comprising trimethoprim at a subinhibitory concentration and a second antimicrobial agent comprising a FolE2 enzyme inhibitor selected from dehydrocostus lactone, parthenolide, or β-lapachone, wherein the combination of the first antimicrobial agent and the second antimicrobial agent exhibits chemical synthetic lethality against Burkholderia pseudomallei while having minimal effect on commensal bacteria. The subinhibitory concentration of trimethoprim ranges between 5 μM and 30 μM. The FolE2 enzyme inhibitor acts as a mechanism-based inhibitor that covalently modifies a catalytic cysteine residue (Cys154) of the FolE2 enzyme. The combination exhibits greater than 90% growth suppression against Burkholderia pseudomallei. Methods for treating melioidosis and identifying antimicrobial drug targets using chemical synthetic lethality screening approaches are also provided.

The invention discloses a trimethoprim / polyacrylic resin solid dispersant as well as a preparation method and application thereof, and belongs to the technical field of veterinary drug preparations. The trimethoprim / polyacrylic resin solid dispersant comprises the following raw materials: trimethoprim and polyacrylic resin, wherein the mass ratio of the trimethoprim to the polyacrylic resin is 0.02 to 0.3; and dissolving the raw materials with an organic solvent, and dialyzing or evaporating to remove the solvent to prepare the trimethoprim / polyacrylic resin solid dispersant. The prepared solid dispersing agent is high in drug loading rate and good in stability, and the formed solid dispersing agent obviously improves the glass transition temperature of trimethoprim. Besides, the preparation method is convenient to operate, simple and easy to implement, and the obtained solid dispersing agent can be compounded with the sulfonamides antibacterial drugs to serve as a synergist of the sulfonamides antibacterial drugs, so that the antibacterial effect of the sulfonamides antibacterial drugs is improved.

The invention discloses a method for removing pollutants in sewage. The method comprises the following steps: S1, mixing sewage, a ferric iron-containing compound and peracetic acid to obtain a mixed solution; s2, irradiating the mixed solution with 222-230nm ultraviolet light to react to remove pollutants; the pollutants comprise at least one of carbamazepine, sulfamethoxazole, benzodiazole and trimethoprim. According to the method, far ultraviolet light (FUV, 222-230 nm) is introduced to strengthen Fe (III) to activate PAA, so that the problem of insufficient reaction activity of a Fe (III) / PAA system is solved.

This invention provides a sulfathiazole-trimethoprim drug crystal salt, its preparation method, and its application, belonging to the field of drug crystal salt technology. The sulfathiazole-trimethoprim drug crystal salt provided by this invention is orthorhombic with a space group of [space group missing]. P bca; cell parameters are: a =17.3213(3)Å, b =12.2894(2)Å, c =23.7549(3)Å, α =90°, β =90°, c =90°; wherein the molar ratio of sulfathiazole to trimethoprim is 1:1, and there is no crystallizing solvent. The sulfathiazole-trimethoprim drug crystal salt provided by this invention has high solubility in water, which is beneficial to improving absorption and metabolism in humans and animals, and has excellent antibacterial effect.

The application discloses a test paper strip and a test method for detecting fluoroquinolones, sulfonamides, tetracyclines and trimethoprim drug residues in aquatic products, and belongs to the technical field of drug detection. A preparation method comprises the following steps: synthesizing a trimethoprim hapten; synthesizing quinolone, sulfonamide and tetracycline haptens; preparing an immunogen and a coating agent; preparing a monoclonal antibody; preparing a gold-labeled antibody; and pretreating and assembling. The application can simultaneously complete the detection of fluoroquinolones, sulfonamides, tetracyclines and trimethoprim residues in aquatic products by constructing a four-class drug integrated detection scheme, and does not need to use different detection tools or batch detection for a single drug. In the actual detection scene of aquatic products, the repeated steps of sample processing and detection operation can be greatly reduced, the waste of aquatic product samples caused by multiple sampling can be avoided, the overall detection period can be shortened, and the rapid quality control demand of aquatic products from fishing / cultivation to circulation can be met.

The invention provides a sulfathiazole-trimethoprim medicine crystal salt as well as a preparation method and application thereof, and belongs to the technical field of medicine crystal salts. The sulfathiazole-trimethoprim drug crystal salt provided by the invention is an orthorhombic crystal system, and the space group is Pbca; the cell parameters are as follows: a is equal to 17.3213 (3), b is equal to 12.2894 (2), c is equal to 23.7549 (3), alpha is equal to 90 degrees, beta is equal to 90 degrees, and gamma is equal to 90 degrees; wherein the molar ratio of sulfathiazole to trimethoprim is 1: 1, and no crystalline solvent exists. The sulfathiazole-trimethoprim drug crystal salt provided by the invention has high solubility in water, is beneficial to improvement of absorption and metabolism conditions in human bodies and animal bodies, and has an excellent antibacterial effect.

The present invention provides a novel pharmaceutical composition of polymyxin B / trimethoprim and rifampicin, as well as a preparation method and topical ocular application thereof (e.g., treatment of ocular infections).

This application provides a *Staphylococcus pseudoci* AT8 strain for degrading antibiotics and its application. The *Staphylococcus pseudoci* is a species of Staphylococcus (…). Staphylococcus The *Staphylococcus pseudocarpa* species, having a 16S rDNA sequence as shown in SEQ ID NO:1, is named... Staphylococcus pseudoxylosus AT8, deposited on November 20, 2025 at the Guangdong Provincial Center for Microbial Culture Collection, accession number: GDMCC No: 67323. This application pertains to *Staphylococcus pseudocarpa*. Staphylococcus pseudoxylosus AT8 can achieve a degradation rate of over 90% for trimethoprim and tilmicosin, and over 78% for enrofloxacin. Furthermore, this application enriches the antibiotic-degrading bacterial strain library, promotes the control of new antibiotic pollutants in livestock and poultry manure and farmland soil, and facilitates the resource-based treatment and safe utilization of solid waste, demonstrating broad application prospects.

The invention relates to a method for synthesizing a diaveridine intermediate, which comprises the following steps: (a) adding dimethyl sulfoxide, potassium hydroxide and 3-dimethylaminopropionitrile into a reaction container, and heating to 65-75 DEG C; then adding veratraldehyde, and stirring to react for 2-3 hours to obtain a first reaction solution; and (b) adding ethanol and aniline into the first reaction solution, then dropwise adding an acid-containing solution, carrying out heat preservation reaction at 75-85 DEG C for 2-3 hours, cooling, crystallizing, and drying to obtain the diaveridine intermediate. Therefore, the side reaction for synthesizing the diaveridine intermediate can be inhibited, so that the reaction conditions are simplified, and the yield of the product is improved.

Described herein are compositions, methods, and kits for detecting antibiotic resistance genes in a sample, such as a wound swab. One embodiment described herein is primer pairs and probes for individual or multiplex polymerase chain reaction (PCR) based assays for the detection of one or more antibiotic resistance gene targets comprising resistance to molecularly characterized extended-spectrum β-lactamases (MESBLs); extended-spectrum β-lactamases (ESBLs); carbapenemase; AmpC β-lactamase; β-lactamase; lincosamide, macrolide, streptogramin; trimethoprim; macrolides; methicillin; colistin; sulfonamide; tetracycline; vancomycin; nitroimidazole; quinolone; or aminoglycoside.

The present invention discloses a herbicide for controlling weeds in quinoa fields and its application, belonging to the technical field of pesticides. The herbicide of the present invention comprises trimethoprim-butyl and trimethosulfuron as active ingredients; the mass ratio of trimethoprim-butyl to trimethosulfuron is 5:1 to 60:1. The herbicide of the present invention is highly safe for quinoa and does not cause phytotoxicity. The combination of trimethoprim-butyl and trimethosulfuron exhibits a significant synergistic effect, exhibiting a significant combined synergistic effect against common weeds in quinoa fields. The herbicidal composition of the present invention expands the weed control spectrum and has excellent control effects against common weeds in quinoa fields.

The invention provides novel pharmaceutical compositions of polymyxin B / trimethoprim and rifampin, as well as methods of their preparation and topical ophthalmic uses (e.g., treatment of ophthalmic infections).

The invention provides a method for preparing a trimethoprim key intermediate, which comprises the following steps: (a) adding an aprotic solvent, methoxypropionitrile and an alkali-alcohol mixed solution into a reaction container, then adding 3, 4, 5-trimethoxybenzaldehyde, heating and stirring for reaction; (b) adding aniline into a product obtained in the step (a), dropwise adding an acid solution to adjust the pH value to 2-3, and continuously heating to react until the TLC detection reaction is finished; (c) cooling and crystallizing the product in the step (b), filtering to obtain a filter cake, and drying to obtain a trimethoprim key intermediate; the chemical structural formula of the trimethoprim key intermediate is shown in the specification. The key intermediate which can be directly used for the next step can be obtained by a one-step method without treatment and at a high conversion rate.

The invention discloses a bimetallic nanoparticle composite graphene portable electrochemical sensor prepared based on a step-by-step laser induction method, and belongs to the field of electrochemical sensing. Sulfamethoxazole (SMX) and trimethoprim (TMP) are selected as research objects, polyimide (PI) is taken as a substrate material, alloy nanoparticles of nickel and copper are introduced into laser induced graphene (LIG) through a two-step laser method, a NiCuNPs / LIG electrode material is prepared, and the electrochemical sensing technology is combined, so that the electrochemical sensing performance of the electrochemical sensor is improved. The laser-induced graphene electrochemical sensor which has excellent sensitivity and excellent selectivity and is used for detecting sulfamethoxazole and trimethoprim is constructed. According to the present invention, the portable analysis detection of the sulfamethoxazole content and the trimethoprim content is achieved, and the novel method for detecting the interaction between the sulfonamide antibiotics and the electrochemical sensor by using the portable element provides the reliable technical support for the detection of the sulfonamide antibiotics under the instant analysis environmental condition, such as the field, the emergency or the special scene.

This invention discloses a method for detecting the bioavailability of antibiotics in soil using continuous solvent extraction-solid phase extraction-liquid chromatography-tandem mass spectrometry. The method first uses continuous solvent extraction to extract the bioactive and extractable antibiotic components from the soil. Then, a solid phase extraction column is used for sample purification. Finally, multiple reaction monitoring (MRM) mode of liquid chromatography-tandem mass spectrometry is used to determine the concentrations of various antibiotics, including chlortetracycline, doxycycline, oxytetracycline, tetracycline, sulfadiazine, sulfadiazine, sulfamethoxazole, ciprofloxacin, enrofloxacin, norfloxacin, ofloxacin, azithromycin, erythromycin, roxithromycin, tylosin, trimethoprim, and lincomycin. This invention combines continuous solvent extraction with solid phase extraction, coupled with liquid chromatography-tandem mass spectrometry detection, to achieve accurate evaluation of trace antibiotic bioavailability under complex matrix conditions.

Formulations and methods for treatment of coccidia infections in an animal. The method includes obtaining a formulation of Statin (e.g., Atorvastatin), Indomethacin, Doxycycline, Cimetidine, Lincomycin, Sulfamethoxazole, Trimethoprim, Diclazuril, Salinomycin, Budesonide, Amprolium, Azithromycin, and / or any combination thereof; and providing the formulation to an animal.

The invention relates to the technical field of biology, in particular to a primer pair, a primer probe composition, a PCR premix, a kit and a method for detecting multiple respiratory pathogens. The invention discloses a primer pair with nucleic acid sequences as shown in SEQ ID NO: 1-2, 4-5, 7-8, 10-11, 13-14, 16-17, 19-20, 22-23, 25-26, 28-29, 31-32, 34-35, 37-38 and 40-41. Aiming at a target respiratory pathogen, a specific amplification primer pair is designed and is matched with a proper probe, so that eight-target joint detection can be realized, and the influence of heme, trimethoprim, sulfamethoxazole, amphotericin B, itraconazole, fluconazole, azithromycin, epinephrine and lidocaine hydrochloride in a detection sample can be avoided in the detection process; the anti-interference performance is good, and cross reaction with rhodococcus equi, candida tropicalis and candida krusei does not exist.

The invention belongs to the technical field of medicine crystallization, and particularly relates to trimethoprim-gentisic acid medicine organic salt and a preparation method and application thereof.The trimethoprim-gentisic acid medicine organic salt single crystal is obtained through a solvent slow volatilization method, and the trimethoprim-gentisic acid medicine organic salt single crystal is obtained through a chemical synthesis method. Trimethoprim-gentisic acid medicine organic salt powder is obtained through grinding or suspension, and the trimethoprim-gentisic acid medicine organic salt powder has excellent wet stability, thermal stability and light stability and better antibacterial effect and antioxidant effect; the solubility difference between trimethoprim and gentisic acid is reduced due to the formation of the medicine organic salt, synchronous release is realized, and synergistic effect is facilitated; the preparation method has the advantages of being simple and convenient to operate, green, safe and the like, and can replace trimethoprim medicine to be applied to treatment of inflammatory diseases caused by bacteria.

The application discloses a method for rapid proliferation and genetic transformation regeneration of Radix Morindae Officinalis embryogenic callus and belongs to the technical field of plant tissue culture. The method comprises the following steps: firstly, inoculating Radix Morindae Officinalis embryogenic callus into a liquid culture medium to carry out suspension culture to obtain Radix Morindae Officinalis embryogenic callus cell groups; then adding the callus cell groups into a bacterial infection liquid to carry out infection; and then transferring the callus cell groups to a differentiation culture medium to carry out culture to obtain Radix Morindae Officinalis transgenic regenerated plants. The suspension culture medium provided by the application can realize large-scale and rapid proliferation of Radix Morindae Officinalis embryogenic callus, and the embryogenic callus cell groups obtained by the proliferation have a good growth state. In the process of establishing the genetic transformation system of Radix Morindae Officinalis, the method of directly carrying out differentiation culture after co-culture is adopted, the differentiation culture medium does not add resistance screening, and an antibacterial agent, i.e., trimethoprim, is added, so that the transgenic plants are regenerated with high efficiency.

The invention provides PEG-PLGA (polyethylene glycol-poly (lactic-co-glycolic acid)) nano drug-loaded particles for enhancing oral absorption of trimethoprim and a preparation method of the PEG-PLGA nano drug-loaded particles, and belongs to the technical field of nano drug-loaded particles. According to the invention, TMP is encapsulated in the degradable PEG-PLGA nano-particles through a water-in-oil emulsion solvent evaporation method, so that the solubility and bioavailability of the degradable PEG-PLGA nano-particles are improved. The nano drug-loaded particles obtained by the method disclosed by the invention have high drug loading capacity (LC) of 34.0 + / -1.6% and encapsulation efficiency (EE) of 88.2 + / -4.3%, the particle size is uniform (245 + / -40nm), and the PDI is as low as 0.103 + / -0.019. The nano drug-loaded particle provided by the invention not only solves the inherent defects (such as low solubility and rapid removal) of TMP, but also remarkably improves the oral bioavailability of the nano drug-loaded particle. The nano drug-loaded particle has the potential of a traditional preparation alternative scheme, and is expected to reduce the drug administration frequency and improve the bacterial infection treatment effect.

The invention discloses application of trichoderma harzianum and metabolite in aspects of plant root promotion and adversity stress resistance, and the preparation method comprises the following steps: inoculating the trichoderma harzianum into a PDB culture medium for culture, and inoculating a culture solution into a rice + catecholamine + trimethoprim culture medium for fermentation. The composition can stimulate the growth of plant root systems, has a relatively obvious promoting effect on crop germination potential and germination rate, promotes the growth of seedlings at the overground part of the crop, accelerates the growth of the top end of the crop, deepens the green color of leaves, enhances the saline-alkali stress resistance of rice, enhances the adversity stress resistance of virus resistance, high temperature resistance, low temperature resistance, drought resistance and waterlogging resistance of the crop, and improves the yield of the rice. Therefore, crops are resistant to adversity and root, and strong induced resistance and immune induced resistance are achieved. The trichoderma harzianum and metabolites prepared by the invention enhance the vitality of crops, are environment-friendly and pollution-free, and have wide application prospects.

The invention discloses a preparation process of an instant trimethoprim solid dispersion with the content of 35% or 40%, the formula of the instant trimethoprim solid dispersion comprises trimethoprim (TMP), a cosolvent and a dispersing agent according to a certain proportion, during preparation, various materials are weighed according to the proportion of the formula, the trimethoprim and the cosolvent are uniformly mixed, and the mixture is put into a mold for molding, so that the instant trimethoprim solid dispersion with the content of 35% or 40% is obtained. And adding purified water, stirring and dissolving the filler, adding the dispersing agent, adding purified water to prepare the material into a solution with the solid content of 15-20%, and drying by adopting a spray dryer to prepare the trimethoprim solid dispersion. The content of trimethoprim in the trimethoprim solid dispersion product is not lower than 35%, the solubility (the content of trimethoprim in the solution) is not lower than 18 mg / mL, and the solubility of trimethoprim is improved under the condition that the content of trimethoprim is guaranteed.