41 results about "Trimethoprim" patented technology

The invention discloses a trimethoprim hapten as well as synthesis and application thereof. The structure of the trimethoprim hapten is as shown in a formula I. The trimethoprim hapten provided by the invention can fully expose the antigenic determinant of trimethoprim and maintain the spatial conformation of trimethoprim, has good immunogenicity, and is mild in synthesis conditions, few in reaction steps, high in yield and capable of reducing the cost of raw materials. The trimethoprim colloidal gold detection product provided by the invention has the characteristics of strong specificity and high sensitivity, can realize accurate and rapid detection of trimethoprim in a sample, and has no cross reaction with other drugs. And the formula I of the # imgabs0 # is shown in the specification.

The invention provides a preparation method of a compound sulfonamide medicinal preparation. The compound sulfonamide medicinal preparation contains a premix prepared from sulfadiazine, sulfamethoxazole, trimethoprim, sodium bicarbonate and a slow-release coating medium. Compared with sulfadiazine, sulfamethoxazole and trimethoprim preparations, the sodium bicarbonate coated compound is high in bioavailability, and after the sodium bicarbonate coated compound is added, caking in urine can be remarkably reduced, and the stability of the preparation is not affected.

This invention provides an anti-trimethoprim single-chain antibody for detecting trimethoprim-like drug residues, its preparation method, and its application. The amino acid sequences of the single-chain antibody are shown in SEQ ID3, SEQ ID4, and SEQ ID6. Compared with traditional monoclonal antibodies, the single-chain antibody has lower production costs, is simpler to prepare, and is easier to manipulate at the gene level. This invention utilizes trimethoprim-specific monoclonal hybridoma cells and employs phage display technology to screen for high-affinity single-chain antibodies. A single-chain antibody mutant with even higher affinity was successfully obtained through computer simulation combined with site-directed mutagenesis. Based on this mutant, an ic-ELISA was established for the detection of trimethoprim residues in animal-derived foods, meeting the needs of practical applications.

The present application belongs to the technical field of wastewater treatment, and particularly relates to a seawater breeding wastewater antibiotic removal method based on oyster shell composite filler. Fine gravel, oyster shell and functional mineral are compounded in a fixed volume ratio of 2:1:1, and are laid in a structure with a limited particle size range and homogeneity without stratification. A salinity-directed salt-tolerant domestication process and a tidal intermittent operation mode of 48h hydraulic retention + 24h emptying and reoxygenation are matched, and salt-tolerant functional bacterial flora of Proteobacteria, Paracoccus and Shigella are enriched. The present application can adapt to a wide range of salinity conditions of 10‰-30‰, relieve high salt inhibition and ion competition, simultaneously and efficiently remove three typical antibiotics of conventional pollutants, sulfamethoxazole, trimethoprim and enrofloxacin, and reduce resistance genes. The sulfamethoxazole removal rate can reach 93.94% under the condition of 30‰ high salinity, the system runs stably and has strong adaptability, and has certain engineering application value and large-scale popularization potential in the field of seawater breeding wastewater treatment.

The invention belongs to the field of analysis and detection, and particularly relates to a sulfamethoxazole and trimethoprim combined trace detection method and application to drug monitoring after hematopoietic stem cell transplantation patient treatment. The detection method comprises the following steps: (1) pre-treating a sample: treating the sample by adopting a releasing agent containing an internal standard substance which comprises an isotope labeled compound; (2) carrying out qualitative and quantitative analysis by adopting liquid chromatography-tandem mass spectrometry, wherein a mobile phase A is an aqueous solution of formic acid; b is methanol. According to the invention, a micro method for detecting the blood concentration of sulfamethoxazole / trimethoprim in a hematopoietic stem cell transplanted child patient by a liquid chromatography-tandem mass spectrometry technology is established; combined determination of sulfamethoxazole and trimethoprim is achieved through a mass spectrum electrospray positive ion multi-reaction monitoring mode, selectivity, residue, accuracy, precision, stability and matrix effect are comprehensively evaluated, and the method is verified completely, stable and reliable.

The invention discloses a test strip for detecting fluoroquinolones, sulfonamides, tetracyclines and trimethoprim drug residues in aquatic products and a test method, and belongs to the technical field of drug detection.The preparation method comprises the steps that trimethoprim hapten is synthesized; synthesizing a quinolone hapten, a sulfanilamide hapten and a tetracycline hapten; preparing an immunogen and a coating antigen; preparing a monoclonal antibody; preparing a gold-labeled antibody; and pre-treating and assembling. According to the invention, an integrated detection scheme of four types of medicines is constructed, so that residual detection of fluoroquinolones, sulfonamides, tetracyclines and trimethoprim in aquatic products can be synchronously completed, and different detection tools or batch detection for single type of medicines is not needed. In an actual detection scene of aquatic products, repeated steps of sample treatment and detection operation can be greatly reduced, waste of aquatic product samples caused by repeated sampling is avoided, meanwhile, the overall detection period is shortened, and the rapid quality control requirement of the aquatic products from fishing / breeding to circulation links is met.

The invention discloses a series connection synthesis method of 3, 4, 5-trimethoxy benzaldehyde. The 3, 4, 5-trimethoxybenzaldehyde is an important medicine and fine chemical engineering intermediate, and can be used for synthesizing a sulfanilamide synergist trimethoprim and an anti-cancer drug etoposide. According to the invention, 3, 5-dibromo-4-hydroxybenzaldehyde is taken as a raw material, a sodium methoxide-methanol solution is taken as a methoxylation reagent, dimethyl carbonate is taken as a methylation reagent, and tandem synthesis of 3, 4, 5-trimethoxybenzaldehyde is realized by adding a plurality of catalysts under normal pressure. The method does not need to separate a reaction intermediate or use a high-pressure reaction container, and has the advantages of simplicity, convenience and safety in operation, low reagent toxicity, higher yield, short reaction time and the like.

The invention provides a method and a kit for detecting blood concentration of compound sulfamethoxazole and metabolite thereof. According to the method, a methanol precipitator is combined with Oasis PRiME HLB reversed-phase solid-phase extraction, the centrifugal process of plasma treatment is reduced, traditional activation and balance steps are omitted, time is saved, meanwhile, the method is more environmentally friendly, and the extraction recovery rate of a to-be-detected substance can be improved; gradient elution is replaced by isocratic elution, the balance returning time is avoided, the overall operation time is shorter, the separation application is not influenced, the operation time is only 6 minutes, all the to-be-measured substances can be accurately and simultaneously measured only by 1 microliter, and the peak appearance time is 2 minutes. According to the invention, trimethoprim, sulfamethoxazole and metabolites thereof sulfamethoxazole-N-acetyl and 4-nitro sulfamethoxazole in human plasma can be monitored at the same time by using a treatment method and a computer method.

The invention discloses an external antibacterial medicine composition as well as a preparation method and application thereof, and belongs to the technical field of antibacterial medicines. The external antibacterial pharmaceutical composition provided by the invention comprises a medicinal active component and a pharmaceutically acceptable carrier, wherein the medicinal active component comprises polymyxin B sulfate, trimethoprim sulfate and lidocaine; every 10 g of the antibacterial pharmaceutical composition for external use is prepared from the following components in parts by weight: 40000 to 60000 U of polymyxin B sulfate, 0.08 to 0.16 g of trimethoprim sulfate and 0.30 to 0.35 g of lidocaine. The pharmaceutical composition disclosed by the invention has broad-spectrum inhibition on multiple drug-resistant bacteria such as common gram-negative drug-resistant bacteria and acinetobacter baumannii on a wound surface, synchronously plays a synergistic role in relieving inflammatory response of the wound surface and relieving local pain, and remarkably improves the medication safety and the comprehensive treatment effect while ensuring excellent antibacterial activity.

This application relates to the field of biotechnology, and particularly to primer pairs, primer-probe compositions, PCR premixes, kits, and methods for detecting multiple respiratory pathogens. Nucleic acid sequences are shown in primer pairs as indicated by SEQ ID NO: 1-2, 4-5, 7-8, 10-11, 13-14, 16-17, 19-20, 22-23, 25-26, 28-29, 31-32, 34-35, 37-38, and 40-41. For target respiratory pathogens, this application designs specific amplification primer pairs, paired with suitable probes, enabling the simultaneous detection of eight targets. Furthermore, the detection process avoids interference from heme, trimethoprim, sulfamethoxazole, amphotericin B, itraconazole, fluconazole, azithromycin, adrenaline, and lidocaine hydrochloride in the test sample. It exhibits good anti-interference properties and shows no cross-reactivity with *Rhodococcus equi*, *Candida tropicalis*, and *Candida krusei*.

The invention discloses an African swine fever virus replication-deficient strain as well as a preparation method and application thereof, and belongs to the technical field of African swine fever viruses. The African swine fever virus replication-deficient strain is obtained by fusing escherichia coli dihydrofolate reductase on p54 protein of African swine fever virus, the nucleotide sequence of the escherichia coli dihydrofolate reductase is as shown in SEQ ID No.1, and the African swine fever virus is an ASFV CN / GS 2018 strain. The African swine fever virus replication-deficient strain is obtained by fusing an unstable structural domain onto a p54 protein, degradation of the p54 protein is regulated and controlled by using a micromolecular ligand trimethoprim, so that replication and proliferation of the virus are regulated and controlled, and the strain has important application values in the aspects of reducing virus toxicity, enhancing vaccine safety, promoting vaccine research and development and the like.

The present disclosure provides a system for treating melioidosis, comprising a first antimicrobial agent comprising trimethoprim at a subinhibitory concentration and a second antimicrobial agent comprising a FolE2 enzyme inhibitor selected from dehydrocostus lactone, parthenolide, or β-lapachone, wherein the combination of the first antimicrobial agent and the second antimicrobial agent exhibits chemical synthetic lethality against Burkholderia pseudomallei while having minimal effect on commensal bacteria. The subinhibitory concentration of trimethoprim ranges between 5 μM and 30 μM. The FolE2 enzyme inhibitor acts as a mechanism-based inhibitor that covalently modifies a catalytic cysteine residue (Cys154) of the FolE2 enzyme. The combination exhibits greater than 90% growth suppression against Burkholderia pseudomallei. Methods for treating melioidosis and identifying antimicrobial drug targets using chemical synthetic lethality screening approaches are also provided.

The invention discloses a trimethoprim / polyacrylic resin solid dispersant as well as a preparation method and application thereof, and belongs to the technical field of veterinary drug preparations. The trimethoprim / polyacrylic resin solid dispersant comprises the following raw materials: trimethoprim and polyacrylic resin, wherein the mass ratio of the trimethoprim to the polyacrylic resin is 0.02 to 0.3; and dissolving the raw materials with an organic solvent, and dialyzing or evaporating to remove the solvent to prepare the trimethoprim / polyacrylic resin solid dispersant. The prepared solid dispersing agent is high in drug loading rate and good in stability, and the formed solid dispersing agent obviously improves the glass transition temperature of trimethoprim. Besides, the preparation method is convenient to operate, simple and easy to implement, and the obtained solid dispersing agent can be compounded with the sulfonamides antibacterial drugs to serve as a synergist of the sulfonamides antibacterial drugs, so that the antibacterial effect of the sulfonamides antibacterial drugs is improved.

The invention discloses a method for removing pollutants in sewage. The method comprises the following steps: S1, mixing sewage, a ferric iron-containing compound and peracetic acid to obtain a mixed solution; s2, irradiating the mixed solution with 222-230nm ultraviolet light to react to remove pollutants; the pollutants comprise at least one of carbamazepine, sulfamethoxazole, benzodiazole and trimethoprim. According to the method, far ultraviolet light (FUV, 222-230 nm) is introduced to strengthen Fe (III) to activate PAA, so that the problem of insufficient reaction activity of a Fe (III) / PAA system is solved.

This invention provides a sulfathiazole-trimethoprim drug crystal salt, its preparation method, and its application, belonging to the field of drug crystal salt technology. The sulfathiazole-trimethoprim drug crystal salt provided by this invention is orthorhombic with a space group of [space group missing]. P bca; cell parameters are: a =17.3213(3)Å, b =12.2894(2)Å, c =23.7549(3)Å, α =90°, β =90°, c =90°; wherein the molar ratio of sulfathiazole to trimethoprim is 1:1, and there is no crystallizing solvent. The sulfathiazole-trimethoprim drug crystal salt provided by this invention has high solubility in water, which is beneficial to improving absorption and metabolism in humans and animals, and has excellent antibacterial effect.

The application discloses a test paper strip and a test method for detecting fluoroquinolones, sulfonamides, tetracyclines and trimethoprim drug residues in aquatic products, and belongs to the technical field of drug detection. A preparation method comprises the following steps: synthesizing a trimethoprim hapten; synthesizing quinolone, sulfonamide and tetracycline haptens; preparing an immunogen and a coating agent; preparing a monoclonal antibody; preparing a gold-labeled antibody; and pretreating and assembling. The application can simultaneously complete the detection of fluoroquinolones, sulfonamides, tetracyclines and trimethoprim residues in aquatic products by constructing a four-class drug integrated detection scheme, and does not need to use different detection tools or batch detection for a single drug. In the actual detection scene of aquatic products, the repeated steps of sample processing and detection operation can be greatly reduced, the waste of aquatic product samples caused by multiple sampling can be avoided, the overall detection period can be shortened, and the rapid quality control demand of aquatic products from fishing / cultivation to circulation can be met.

The invention discloses a culture medium for detecting streptococcus agalactiae in bovine mastitis and a test piece thereof, the culture medium comprises a nutritional ingredient mixture, a composite inhibitor, a composite chromogenic enzyme substrate and cold water gel, the composite inhibitor is aztreonam, nalidixic acid, potassium clavulanate and trimethoprim; the compound chromogenic enzyme substrate is 5-bromine-6-chloro-3-indole-phosphoric acid p-toluidine, 5-bromine-4-chloro-3-indole-beta-D-glucuronide, 5-bromine-6-chloro-3-indole-beta-D-glucopyranoside, and 5-bromine-6-chloro-3-indole-beta-D-galactoside, and the compound chromogenic enzyme substrate is a compound chromogenic enzyme. The composite inhibitor in the streptococcus agalactiae test piece can effectively inhibit growth of non-target bacteria, and the composite chromogenic enzyme substrate has higher selectivity on target bacteria, so that the test piece has the characteristics of high specificity, high sensitivity, short detection period, easiness in observation and low detection cost; the method has huge development potential and wide application prospect in pasture detection.

The present invention provides a trimethoprim derivative, a preparation method thereof, a label protein based on the trimethoprim derivative and an application thereof. The preparation method comprises reacting a compound A#imgabs0# with a substituted amine compound H 2 N‑CH 2 ‑(CH 2 ) n ‑R, wherein n is selected from any natural number of 1 to 5, and R is selected from halogen, and an O-alkylation reaction is performed to obtain compound B#imgabs1# Compound B is subjected to a reductive amination reaction to introduce a benzene ring into compound B to obtain compound C#imgabs2# R2 is an amino protecting group; Compound C is subjected to a coupling reaction with an acrylic acid compound to obtain a trimethoprim derivative TMP3‑NH 2 #imgabs3# Labeling based on this trimethoprim derivative helps to increase the labeling rate.

The invention provides a sulfathiazole-trimethoprim medicine crystal salt as well as a preparation method and application thereof, and belongs to the technical field of medicine crystal salts. The sulfathiazole-trimethoprim drug crystal salt provided by the invention is an orthorhombic crystal system, and the space group is Pbca; the cell parameters are as follows: a is equal to 17.3213 (3), b is equal to 12.2894 (2), c is equal to 23.7549 (3), alpha is equal to 90 degrees, beta is equal to 90 degrees, and gamma is equal to 90 degrees; wherein the molar ratio of sulfathiazole to trimethoprim is 1: 1, and no crystalline solvent exists. The sulfathiazole-trimethoprim drug crystal salt provided by the invention has high solubility in water, is beneficial to improvement of absorption and metabolism conditions in human bodies and animal bodies, and has an excellent antibacterial effect.

The present invention provides a novel pharmaceutical composition of polymyxin B / trimethoprim and rifampicin, as well as a preparation method and topical ocular application thereof (e.g., treatment of ocular infections).

This application provides a *Staphylococcus pseudoci* AT8 strain for degrading antibiotics and its application. The *Staphylococcus pseudoci* is a species of Staphylococcus (…). Staphylococcus The *Staphylococcus pseudocarpa* species, having a 16S rDNA sequence as shown in SEQ ID NO:1, is named... Staphylococcus pseudoxylosus AT8, deposited on November 20, 2025 at the Guangdong Provincial Center for Microbial Culture Collection, accession number: GDMCC No: 67323. This application pertains to *Staphylococcus pseudocarpa*. Staphylococcus pseudoxylosus AT8 can achieve a degradation rate of over 90% for trimethoprim and tilmicosin, and over 78% for enrofloxacin. Furthermore, this application enriches the antibiotic-degrading bacterial strain library, promotes the control of new antibiotic pollutants in livestock and poultry manure and farmland soil, and facilitates the resource-based treatment and safe utilization of solid waste, demonstrating broad application prospects.

The invention relates to a method for synthesizing a diaveridine intermediate, which comprises the following steps: (a) adding dimethyl sulfoxide, potassium hydroxide and 3-dimethylaminopropionitrile into a reaction container, and heating to 65-75 DEG C; then adding veratraldehyde, and stirring to react for 2-3 hours to obtain a first reaction solution; and (b) adding ethanol and aniline into the first reaction solution, then dropwise adding an acid-containing solution, carrying out heat preservation reaction at 75-85 DEG C for 2-3 hours, cooling, crystallizing, and drying to obtain the diaveridine intermediate. Therefore, the side reaction for synthesizing the diaveridine intermediate can be inhibited, so that the reaction conditions are simplified, and the yield of the product is improved.

Described herein are compositions, methods, and kits for detecting antibiotic resistance genes in a sample, such as a wound swab. One embodiment described herein is primer pairs and probes for individual or multiplex polymerase chain reaction (PCR) based assays for the detection of one or more antibiotic resistance gene targets comprising resistance to molecularly characterized extended-spectrum β-lactamases (MESBLs); extended-spectrum β-lactamases (ESBLs); carbapenemase; AmpC β-lactamase; β-lactamase; lincosamide, macrolide, streptogramin; trimethoprim; macrolides; methicillin; colistin; sulfonamide; tetracycline; vancomycin; nitroimidazole; quinolone; or aminoglycoside.

The present invention discloses a herbicide for controlling weeds in quinoa fields and its application, belonging to the technical field of pesticides. The herbicide of the present invention comprises trimethoprim-butyl and trimethosulfuron as active ingredients; the mass ratio of trimethoprim-butyl to trimethosulfuron is 5:1 to 60:1. The herbicide of the present invention is highly safe for quinoa and does not cause phytotoxicity. The combination of trimethoprim-butyl and trimethosulfuron exhibits a significant synergistic effect, exhibiting a significant combined synergistic effect against common weeds in quinoa fields. The herbicidal composition of the present invention expands the weed control spectrum and has excellent control effects against common weeds in quinoa fields.

The invention discloses a multi-type veterinary drug mixed standard substance as well as a preparation method and application thereof, and the preparation method comprises the following steps: (1) screening veterinary drug candidates: selecting representative veterinary drugs which are wide in application range, diversified in detection method and easy to cause harm to human bodies, comprising difloxacin, florfenicol, sulfadimidine, trimethoprim, thiamphenicol and fenvalerate. And (2) mixing the selected veterinary drugs in proportion: mixing the difloxacin, the florfenicol, the sulfadimidine, the trimethoprim, the thiamphenicol and the fenvalerate in proportion. The multi-type veterinary drug mixed standard substance developed by the invention has a certain quantity value, can be used for simultaneously measuring multiple veterinary drugs, and can be stably stored at-20 DEG C for 6 months. According to the multi-type veterinary drug mixed standard substance, the detection accuracy of various veterinary drugs under different platforms can be improved; the method can be applied to the detection process of various veterinary drugs, and the accuracy and efficiency of veterinary drug residue detection are improved.

The invention provides novel pharmaceutical compositions of polymyxin B / trimethoprim and rifampin, as well as methods of their preparation and topical ophthalmic uses (e.g., treatment of ophthalmic infections).

The invention provides a method for preparing a trimethoprim key intermediate, which comprises the following steps: (a) adding an aprotic solvent, methoxypropionitrile and an alkali-alcohol mixed solution into a reaction container, then adding 3, 4, 5-trimethoxybenzaldehyde, heating and stirring for reaction; (b) adding aniline into a product obtained in the step (a), dropwise adding an acid solution to adjust the pH value to 2-3, and continuously heating to react until the TLC detection reaction is finished; (c) cooling and crystallizing the product in the step (b), filtering to obtain a filter cake, and drying to obtain a trimethoprim key intermediate; the chemical structural formula of the trimethoprim key intermediate is shown in the specification. The key intermediate which can be directly used for the next step can be obtained by a one-step method without treatment and at a high conversion rate.

The invention discloses a bimetallic nanoparticle composite graphene portable electrochemical sensor prepared based on a step-by-step laser induction method, and belongs to the field of electrochemical sensing. Sulfamethoxazole (SMX) and trimethoprim (TMP) are selected as research objects, polyimide (PI) is taken as a substrate material, alloy nanoparticles of nickel and copper are introduced into laser induced graphene (LIG) through a two-step laser method, a NiCuNPs / LIG electrode material is prepared, and the electrochemical sensing technology is combined, so that the electrochemical sensing performance of the electrochemical sensor is improved. The laser-induced graphene electrochemical sensor which has excellent sensitivity and excellent selectivity and is used for detecting sulfamethoxazole and trimethoprim is constructed. According to the present invention, the portable analysis detection of the sulfamethoxazole content and the trimethoprim content is achieved, and the novel method for detecting the interaction between the sulfonamide antibiotics and the electrochemical sensor by using the portable element provides the reliable technical support for the detection of the sulfonamide antibiotics under the instant analysis environmental condition, such as the field, the emergency or the special scene.

The invention provides novel pharmaceutical compositions of polymyxin B / trimethoprim and rifampin, as well as methods of their preparation and topical ophthalmic uses (e.g., treatment of ophthalmic infections).

The present invention provides a medium for detecting Bacillus cereus groups, which can be easily produced, has good growth of Bacillus cereus regardless of temperature conditions, and has excellent selectivity, and a method for detecting Bacillus cereus groups using the medium. The medium for detecting Bacillus cereus groups contains a phosphatidylinositol-specific phospholipase C substrate and trimethoprim, and the phosphatidylinositol-specific phospholipase C substrate has a color-developing or fluorescent free radical that can be detected.