3 results about "Ocular Infections" patented technology

This invention, entitled "Primer and Probe Composition, Kit, and Application for Detecting Ocular Infectious Pathogens," belongs to the field of molecular biology detection technology. The technical problems it aims to solve are cumbersome single-detection procedures, cross-reactivity in multiple detections, and insufficient sensitivity. The key technical solution is to provide a primer and probe composition for detecting ocular infectious pathogens. This composition includes primers and probes for detecting the CMV UL123 gene, the AdV Hexon gene, and the EBV EBNA-1 gene, along with internal quality control primers and probes, and employs four different fluorescent labels. This invention also provides a kit containing the above primer and probe composition. The kit enables simultaneous qualitative and quantitative detection of three pathogens: CMV, AdV, and EBV, with high accuracy and sensitivity, suitable for early diagnosis, disease monitoring, and prognostic assessment of ocular infections.

This invention discloses an injectable AuPd-Fe single-atom nanozyme composite drug-derived antibacterial hydrogel, its preparation method, and its applications, belonging to the field of biomedical nanomaterials technology. This invention uses an iron-based single-atom nanozyme with an irregular octahedral carbon framework structure as the core catalytic carrier, and in situ loads ultra-small AuPd alloy nanoparticles onto its surface to obtain the AuPd-Fe single-atom nanozyme. Then, using the AuPd-Fe single-atom nanozyme as the active center and oleanolic acid drug-derived hydrogel as the delivery carrier, the AuPd-Fe single-atom nanozyme is uniformly dispersed in the three-dimensional network of the OA hydrogel through physical embedding and hydrogen bonding, resulting in the injectable AuPd-Fe single-atom nanozyme composite drug-derived antibacterial hydrogel. The provided antibacterial hydrogel exhibits excellent ocular surface biocompatibility, no significant corneal toxicity, and no systemic organ damage. It also shows significant clinical application potential for blinding ocular infections such as drug-resistant bacterial keratitis and corneal ulcers.

This invention, entitled "A Primer-Probe Combination for Simultaneous Detection of AMB, VZV, and HSV-1, and its Detection Method, Application, and Product," belongs to the field of molecular biology detection technology. The technical problem it aims to solve is that existing ocular infection detection technologies cannot simultaneously detect AMB, VZV, and HSV-1 in a single tube, or only cover the virus and miss AMB, or require multiple tubes, have unclear identification, insufficient sensitivity, and are difficult to adapt to small ocular samples, failing to meet the needs of rapid and accurate clinical diagnosis. The key technical solution is to provide specific primers and probes targeting the 18S rRNA gene of AMB, the ORF62 gene of VZV, and the UL30 gene of HSV-1, combined with internal quality control primers and probes, using four different fluorescent labels to establish a single-tube multiplex real-time fluorescent PCR detection method, and preparing a matching kit to achieve simultaneous detection and identification of the three pathogens. This invention features high sensitivity, specificity, and strong anti-interference ability.