The invention discloses a primer probe combination for simultaneously detecting AMB, VZV and HSV-1 as well as a detection method, application and a product of the primer probe combination, and belongs to the technical field of molecular biological detection. The technical problems to be solved are that an existing
eye infection detection technology cannot synchronously detect AMB, VZV and HSV-1 in a
single tube, or only covers viruses and misses AMB, or needs multi-tube operation, is fuzzy in identification, insufficient in sensitivity, difficult to adapt to eye trace samples, and cannot meet clinical rapid and accurate diagnosis requirements. According to the technical scheme, the method is characterized in that specific primer probes aiming at 18S rRNA genes of AMB, ORF62 genes of VZV and UL30 genes of HSV-1 are provided and matched with
internal quality control primer probes, four different
fluorescence labels are adopted, a single-tube multiple real-time
fluorescent PCR detection method is established, a matched kit is prepared, and
synchronous detection and identification of three pathogens are achieved. The kit is high in sensitivity and strong in specificity and anti-interference capability.