49 results about "Keratitis" patented technology

The invention relates to eye drops containing exosomes and a preparation method and application thereof, and belongs to the technical field of eye drop preparation, the eye drops comprise exosome freeze-dried powder, the exosome freeze-dried powder is prepared from the exosomes obtained after mesenchymal stem cells are treated with TNF-alpha and IFN-alpha, and a herba houttuyniae extract, the eye drops provided by the invention can inhibit the phenotype of macrophage M1 and promote the M1 to be converted into the phenotype of M2; human corneal cell proliferation, migration and damage repair are promoted; mouse cornea injury repair is accelerated; the eye drops can be used for treating xerophthalmia, conjunctivitis and keratitis, and can also be used for preventing eye diseases and relieving asthenopia; the eye drops disclosed by the invention contain the exosome obtained after the mesenchymal stem cells are treated by the TNF-alpha and the IFN-alpha, so that the anti-inflammatory ability is stronger; the mesenchymal stem cell exosome and the herba houttuyniae extract are stored in a freeze-dried form, so that the activity is stable, and the storage time is long; in addition, the cordate houttuynia is cooperated with the exosome, so that the effect playing time can be prolonged, and the use frequency is reduced.

The invention belongs to the technical field of microorganisms, and particularly relates to bifidobacterium animalis for relieving eye allergy and application of the bifidobacterium animalis. The invention provides an application of bifidobacterium animalis subsp. Lactis in preparation of a product for relieving eye allergy, the bifidobacterium animalis subsp. Lactis is Def +, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.35798. The Bifidobacterium animalis subsp. Lactis Def < + > provided by the invention can relieve the eye itching symptom, relieve red and swollen eyelids, reduce conjunctiva and cornea injury, relieve eye discomfort, relieve globular conjunctivitis cell infiltration, improve corneal epithelium thickening lesion, improve the anti-inflammatory ability and improve allergic conjunctivitis, keratitis and blepharitis marginata in a targeted manner. The eye drops are high in safety and resistant to gastrointestinal fluid, and a safe and long-acting scheme is provided for eye allergic diseases.

The invention discloses a semi-supervised keratitis IVCM image classification method, system and device based on a median enhanced attention mechanism and a medium, and the method comprises the steps: carrying out the class prediction of IVCM images with labels, weak enhancement and no labels after strong enhancement, and obtaining corresponding class score vectors; according to the category score vector with the label, obtaining label loss; according to the category score vector without the label after weak enhancement and a confidence coefficient threshold value of the current training round, defining a pseudo label, and obtaining a maximum category probability of the pseudo label; calculating no-label loss according to the category score vector of the no-label after strong enhancement and the maximum category probability of the pseudo-label; training the MEWRN network in combination with the label loss and the label-free loss; systems, devices, and media for implementing the method; the accuracy of the classification model is improved by adopting the label-free data and the label data; a median-enhanced attention mechanism is introduced into the MEWRN, so that the extraction effect of image features is enhanced, and accurate classification is realized.

The application discloses an ophthalmic disease image classification system based on a big data model, which comprises a data set construction module, an image segmentation module and a model training module.The data set construction module is used for collecting eye images of patients with different ophthalmic diseases in an ophthalmic clinic and performing image marking processing to construct training and test data sets.The image segmentation module adopts YOLOv7 to perform image segmentation on the images taken by the smart phone to obtain eye images containing only the part below the eyebrows and above the zygomatic arch.The model training module adopts a five-fold cross-validation method to train the model on the images, and applies data enhancement technology, white balance adjustment and a transfer learning algorithm.The application relates to the technical field of ophthalmic disease image classification.The ophthalmic disease image classification system based on the big data model has high classification accuracy for diseases such as cataracts, keratitis and pterygium in the development stage test set and different clinical evaluation stages.

The utility model discloses a portable eyeshade capable of preventing exposed keratitis, which comprises a main body unit and a treatment unit, the main body unit comprises an eyeshade body, an elastic band is fixed on the eyeshade body, two through grooves are arranged in the eyeshade body, and the eyeshade body is provided with an adjusting component for adjusting whether shading is carried out or not. The eyeshade comprises two vaseline moisturizing dressings fixed on an eyeshade body, and sterile hydrophilic dressings are fixed on one sides of the vaseline moisturizing dressings. The medicine bag is placed in the storage cavities in the vaseline moisturizing dressing and the sterile hydrophilic dressing, then the vaseline moisturizing dressing is turned over so that the vaseline moisturizing dressing can block the through groove, the medicine bag can treat the eye, the vaseline moisturizing dressing is fixed by embedding the positioning column into the cylinder, namely, the medicine bag is fixed, and the medicine bag can be used for treating the eye. After the shifting rod moves upwards to enable the positioning column to move out of the cylinder body, the vaseline moisturizing dressing can be turned over from the through groove, and the eyes of the patient can be observed through the through groove.

This invention discloses the application of ferrous ions in the preparation of products for treating bacterial infections, belonging to the field of pharmaceutical technology. Antibacterial activity tests of ferrous ions against Staphylococcus aureus showed strong bactericidal effects, with a survival rate of less than 0.001% against Staphylococcus aureus and less than 0.01% against methicillin-resistant Staphylococcus aureus (MRSA). The use of ferrous ion-containing hydrogels in the treatment of keratitis and skin wound infections significantly reduced the risk of MRSA infection in the lungs and effectively prevented the proliferation and spread of microorganisms in the body, revealing that drugs containing ferrous compounds can be used to treat Staphylococcus aureus infections, including MRSA.

The invention discloses an ophthalmology image classification method and device based on global local feature extraction, equipment and a medium, and relates to the technical field of medical image analysis, and the method comprises the steps: obtaining a to-be-classified high-resolution cornea image; inputting the to-be-classified high-resolution cornea image into a preset keratitis image classification model so as to perform fungal image classification on the to-be-classified high-resolution cornea image through the preset keratitis image classification model, and outputting a corresponding aspergillus keratitis image classification result or fusarium keratitis image classification result; wherein the preset keratitis image classification model is an image classification model which learns multi-scale fungus feature extraction capability, fuses image global features and image local features and performs image classification. And fine classification of the two types of fungi is realized through the model.

This invention belongs to the field of molecular diagnostics, specifically relating to biomarkers for the diagnosis of herpes simplex keratitis (HSK) and their applications. This invention is the first to demonstrate that C1QA and FCERT1G are specific and reliable inflammation-related biomarkers for HSK. Diagnostic and assessment methods based on these biomarkers can provide crucial molecular evidence for the early detection, accurate diagnosis, objective severity grading, and personalized treatment of HSK. Simultaneously, this invention reveals the potential roles of FCERT1G and C1QA in HSK and provides new ideas for targeted therapy of this disease, particularly the application of goserelin as an inflammation modulator, offering a new direction for HSK treatment.

To provide a treatment and a diagnosis of autoantibody-mediated eye diseases.SOLUTION: The present disclosure provides an ophthalmic formulation comprising one or more pharmaceutically acceptable excipients; a pharmaceutically active compound that is capable of reducing the amount or deleterious actions of autoantibodies on the ocular surface, such as IgG. In particular, the present disclosure provides an ophthalmic formulation where the pharmaceutically active compound is capable of treating a clinical condition selected from the group consisting of inflammatory, infectious and immunological ocular surface or intraocular disease that can cause symptoms of ocular discomfort, keratitis, dry eye disease, symblepheron formation, fornix foreshortening, eyelid margin / conjunctival keratinization, subconjunctival fibrosis, retinal gliosis and glaucoma.SELECTED DRAWING: None

The invention provides an acanthamoeba protozoa nano antibody, which is characterized in that the acanthamoeba protozoa nano antibody comprises complementary determining regions CDR1, CDR2 and CDR3, the amino acid sequence of the CDR1 is as shown in SEQ ID NO.1, the amino acid sequence of the CDR2 is as shown in SEQ ID NO.2, and the amino acid sequence of the CDR3 is as shown in SEQ ID NO.3. The nano antibody aiming at the acanthamoeba protozoa is obtained by screening on the basis of an alpaca natural antibody library, and the nano antibody is excellent in solubility, stability, resistance to aggregation, refolding performance and expression yield, low in expression cost and capable of being widely applied and popularized to pathogen detection of tap water, swimming pools, contact lens boxes and clinical patient specimens. Meanwhile, a targeted drug developed based on the antibody makes up the blank of treatment of amebic keratitis at present, and has important clinical value and market potential.

The invention provides a training method of a keratitis grading model fusing focus and complication image blocks, and relates to the field of artificial intelligence, and the method comprises the steps: collecting a plurality of slit lamp image samples; for each slit lamp image sample, identifying a cornea region and a conjunctiva region in the slit lamp image sample by using an image positioning algorithm; randomly cutting at least one first image block in each of upper, lower, left and right boundary areas of the conjunctiva area; randomly cutting at least one second image block in the cornea area; inputting the first image blocks and the second image blocks into a DenseNet121 model in sequence to obtain image features of the image blocks; cascading the image features to obtain a first cascading feature; performing dimension reduction on the first cascade feature by using a PCA algorithm to obtain a first dimension reduction feature; and performing iterative training on the cornea classifier based on the first dimension reduction feature until the cornea classifier converges. According to the method, the performance of the keratitis grading model and the keratitis grading accuracy are improved.

The present invention discloses a preparation method and application of a metal ion coordination gel, wherein the gel material comprises: H-(RADA)4 and (RADA)4 gel stock solution, through histidine (His) and Cu 2+ The resulting complex solution, H-(RADA)4-Cu, is then mixed evenly with the (RADA)4 solution and added to a PBS buffer solution to form a stable gel. This gel exhibits excellent biocompatibility, promotes corneal epithelial cell repair, inhibits the growth of Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli), and is effective in treating keratitis.

The present invention contemplates a topical ophthalmic system for treating a mammalian eye infected with a gram-positive bacteria. The system includes an ophthalmic composition comprising a halogenated fluorescein or a pharma- ceutically acceptable salt or ester thereof dissolved or dispersed in an aqueous ophthalmic carrier and present in a concentration effective for treating antibacterial keratitis of about 0.2 μg / mL to about 50 μg / mL. The ophthalmic composition has a pH value of about 6.5 to about 7.6, a viscosity of about 10 to about 300 cps, and an osmolality of about 270 mOsm / kg to about 340 mOsm / kg. The ophthalmic composition is present in a container opaque to actinic radiation. Also contemplated is a method of treating a mammal suffering from an ophthalmic gram-positive bacteria infection by administering the ophthalmic composition to the infected eye and maintaining the treated eye substantially free of actinic radiation for about 3 to about 12 hours.

The present disclosure provides an ophthalmic formulation comprising: one or more pharmaceutically acceptable excipients; a pharmaceutically active compound capable of reducing the amount or deleterious effects of autoantibodies on the ocular surface, such as IgG; in particular, the present disclosure provides an ophthalmic formulation wherein the pharmaceutically active compound is capable of treating a clinical condition selected from the group consisting of inflammatory, infectious and immunological ocular surface or intraocular diseases that can cause symptoms of ocular discomfort, keratitis, dry eye disease, formation of symblepharon, shortening of the anterior chamber depth, lid margin / conjunctival keratinization, subconjunctival fibrosis, retinal gliosis and glaucoma.

According to the system and method for detecting corneal pathogens, in the system and method, the adsorption capacity is enhanced through ionic liquid modified MXene, silk fibroin in silk fibroin composite hydrogel has good biocompatibility, pathogens on the surface of the cornea can be accurately extracted after compounding, the bacterial adsorption efficiency reaches 92.3 + / -2.7%, and the bacterial adsorption efficiency reaches 92.3 + / -2.7%. The adsorption efficiency of fungi reaches 85.4 + / -3.8%, the adsorption efficiency of viruses reaches 88.6 + / -3.5%, high adsorption efficiency is achieved, and non-invasive sampling is achieved. The combined use of the micro-fluidic chip and the LAMP technology can accurately identify the nucleic acid sequence of a target pathogen, the detected pathogen can be judged only according to the detected fluorescence signal or color change, the pathogen detection can be rapidly completed within 1 h, the detection sensitivity reaches 101-102 CFU / mL, high-sensitivity, rapid and non-invasive detection is realized, and the detection cost is reduced. The kit is suitable for early diagnosis of keratitis.

Methods for sensitive and reliable qualitative detection and identification of the common pathogens associated with ocular infections, including uveitis, endophthalmitis and keratitis.

The presently disclosed subject matter is directed to compositions and methods for treating CaMKK2-mediated ophthalmic diseases, including but not limited to 1) ocular surface inflammatory diseases (OSIDs), including but not limited to ocular graft versus host disease, ocular cicatricial pemphigoid, vernal keratoconjunctivitis, allergic eye disease, meibomian gland dysfunction, aqueous tear deficiency (common dry eye disease), corneal scarring, and conjunctival scarring and fibrosis; 2) uveitis and other inflammatory diseases of the eye, including but not limited to keratitis, scleritis, iritis, iridocyclitis, intermediate uveitis, pars planitis, posterior uveitis, choroiditis, chorioretinitis, retinitis, or panuveitis of noninfectious, infectious, or idiopathic etiologies; and 3) “back of the eye” retinal diseases, which include dry age-related macular degeneration, neovascular age-related macular degeneration, diabetic retinopathy, retinal vascular diseases (e.g. retinal vein occlusion, retinal artery occlusion), and retinal degenerations and dystrophies, in a subject. Particularly, the disclosed compounds exhibit improvements over STO-609, a well characterized specific inhibitor of CaMKK2. The disclosed compounds exhibit enhanced aqueous solubility and formulation, as well as elimination of non-binding isomers during production. The disclosed inhibitor compounds can be used to effectively treat ophthalmic diseases, cancers, appetite disorders, systemic inflammatory diseases, and the like.

The utility model relates to the technical field of ophthalmology nursing, and belongs to an eyewash device for keratitis nursing, which comprises a main body, an eyewash basin is arranged in the main body, an injection pipe is arranged in the eyewash basin, and the injection pipe is connected with an external water pump and a heating instrument through pipelines; the injection pipe is used for flushing eyes; a flushing assembly is arranged in the eye washing basin and used for flushing the eye washing basin. A driving assembly is arranged in the main body and is in transmission fit with the flushing assembly to enable the flushing assembly to rotate; a wastewater recycling assembly is arranged in the main body. Cleaning water flow is heated through the heating instrument, the comfort degree of cleaning the eyes is improved, and the situation that the water temperature is too high or too low to stimulate the eyes is prevented; the washing assembly is driven to rotate through the driving assembly, the washing assembly is used for washing the inner wall of the eye washing basin, and secreta washing liquid is prevented from remaining on the inner wall of the eye washing basin to generate cross infection.

The present invention discloses the use of ferrous ions in the preparation of products for treating bacterial infections, belonging to the field of medical technology. Ferrous ions were used in antibacterial activity tests on Staphylococcus aureus, demonstrating a strong bactericidal effect. When sterilizing Staphylococcus aureus, the survival rate was less than 0.001%, and when sterilizing methicillin-resistant Staphylococcus aureus (MRSA), the survival rate was less than 0.01%. The use of ferrous ion-containing hydrogels in the treatment of keratitis and skin wound infections significantly reduced the risk of MRSA lung infection and effectively and timely prevented the reproduction and spread of microorganisms in the body. This indicates that drugs containing ferrous compounds can be used to treat Staphylococcus aureus infections, including MRSA.

The present invention relates to a method for classifying keratitis activity using artificial intelligence, comprising: an image receiving step of receiving an anterior segment image of a subject; a preprocessing step of obtaining a pupil region image from the received anterior segment image; and a training step of training an artificial intelligence model using the pupil region image labeled with the activity level of keratitis.

The invention provides contact lens lubricating liquid containing lysozyme and recombinant collagen as well as a preparation method and application of the contact lens lubricating liquid, and belongs to the technical field of eye care products. The contact lens lubricating liquid provided by the invention comprises the following components: 0.01%-0.5% of lysozyme, 0.1%-2.0% of recombinant collagen, 0.5%-1.2% of an osmotic pressure regulator, 0.01%-0.1% of a viscosity regulator and 0.1%-2.0% of a pH buffer agent. The lysozyme and the recombinant collagen are taken as core active components, and the lysozyme and the recombinant collagen have a synergistic effect, so that the gel has the functions of resisting bacteria, preserving moisture, repairing cornea and improving wearing comfort. The invention further provides a preparation method of the contact lens lubricating liquid and application of the contact lens lubricating liquid in preparation of products for relieving xerophthalmia and preventing contact lens related keratitis.

The invention discloses a steam eye patch and a preparation method thereof, the steam eye patch comprises an eye patch and a traditional Chinese medicine matrix liquid, the traditional Chinese medicine matrix liquid comprises the following components in parts by mass: 20-50 parts of wild chrysanthemum, 20-50 parts of golden cypress, 10-50 parts of coptis chinensis, 1-5 parts of salvia miltiorrhiza, 1-5 parts of ginseng, 1-5 parts of dendrobe, 0.00001-1 part of natural borneol, 0.00001-1 part of menthol and 0.5-2 parts of xanthan gum. The eye pad prepared by the invention combines the dual effects of physical hot compress and traditional Chinese medicine components, and various experiments prove that the eye pad can relieve meibomian gland atrophy and blockage, relieve conjunctivitis, keratitis and other inflammatory reactions, improve tear secretion and reduce xerophthalmia, and is very suitable for eye care.

The present invention discloses a pH response contact lens and a preparation mold thereof, and relates to the technical field of contact lens preparation, the pH response contact lens comprises a drug storage layer lens and a covering layer lens, the covering layer lens covers the drug storage layer lens and is located on the inner side of the drug storage layer lens, the covering layer lens is automatically dissolved or degraded when the pH value of tear is higher than a specific threshold value, and at the moment, liquid medicine stored in the medicine storage layer lens flows out. Through mutual cooperation of the medicine storage layer lenses and the covering layer lenses, the covering layer lenses are automatically dissolved when the eyes of a human body are inflamed and the pH value is higher than a normal range, liquid medicine stored in the medicine storage layer lenses automatically flows out, and the precise treatment function of keratitis is achieved.

The invention belongs to the technical field of medical instruments, and particularly discloses a keratitis tear detection kit which comprises a shell, the shell is of a cylindrical structure with an opening in the lower end, through grooves are symmetrically formed in the outer wall of the shell, a center shaft is slidably arranged at the center of the upper end of the shell in a penetrating mode, and a downward-pressing pushing assembly is arranged on the lower wall of the center shaft and the lower wall of the shell. The upper end of the center shaft and the upper wall of the shell are provided with pressing assemblies, the through groove of the shell is provided with an eyelid opening assembly, the lower end of the center shaft and the lower portion of the inner wall of the shell are provided with an eye anastomosis mechanism, the eye anastomosis mechanism comprises an anastomosis linkage assembly, an eye anastomosis head and detection test paper, and the eye anastomosis head is arranged at the lower end of the center shaft. The anastomosis linkage assembly is arranged on the inner wall of the eye anastomosis head and the inner wall of the shell, and the detection test paper is installed on the eye anastomosis head. By pressing the pressing head downwards, the upper eyelids and the lower eyelids of a patient can be kept in an open state, meanwhile, the detection test paper is matched with the eyeballs of the patient in shape, and collection and detection of tears are better completed.

This invention provides a contact lens lubricant containing lysozyme and recombinant collagen, its preparation method, and its application, belonging to the technical field of eye care products. The contact lens lubricant provided by this invention comprises the following components: 0.01%–0.5% lysozyme, 0.1%–2.0% recombinant collagen, 0.5%–1.2% osmotic pressure regulator, 0.01%–0.1% viscosity regulator, and 0.1%–2.0% pH buffer. This invention uses lysozyme and recombinant collagen as the core active ingredients; their synergistic effect provides antibacterial, moisturizing, corneal repair, and improved wearing comfort functions. This invention also provides a method for preparing the above-mentioned contact lens lubricant and its application in the preparation of products for relieving dry eye syndrome and preventing contact lens-related keratitis.

The invention relates to the technical field of ophthalmic material preparation, and particularly discloses a collagen-containing composite material and application thereof in preparation of a corneal injury repair material. The collagen-containing composite material is prepared from collagen, polysaccharide and a plant extract, wherein the weight ratio of the collagen to the polysaccharide to the plant extract is 100: (5-15): (1-5). Researches show that the collagen-containing composite material disclosed by the invention has the effect of resisting pseudomonas aeruginosa; therefore, the corneal injury repair material prepared from the collagen-containing composite material can be used for preventing the occurrence of pseudomonas aeruginosa keratitis after being used for corneal surgical repair; the important application value is realized.

The application relates to the field of ophthalmic drugs, and particularly discloses an eye drop, which comprises the following components in mass fraction: 0.003%-0.2% of chlorhexidine gluconate; and 0.025%-0.05% of chloramphenicol. Since acanthamoeba usually feeds on bacteria, the chlorhexidine gluconate is used as a main component to kill acanthamoeba in the application, and the antibiotic chloramphenicol is added, so that the keratitis caused by bacterial infection can be avoided, the ocular surface bacteria can be killed, the nutrition source of acanthamoeba can be reduced, and the treatment of acanthamoeba keratitis can be synergistically strengthened.

The present invention relates to a stable formulation based on polyhexamethylene biguanide (PHMB) suitable for ocular level administration, a process for its preparation and a relative dosage regimen effective in the treatment of Acanthamoeba keratitis and in particular in the eradication of cysts.

The invention discloses a construction method and system of a herpes simplex keratitis infection model. The method comprises the following steps: fixing a mouse in a mild anesthesia state and enabling eyeballs of the mouse to protrude as much as possible, sucking an HSV-1 virus suspension by using a microsyringe, obliquely inserting a needle from the cornea edge close to the sclera, keeping the inclined surface of the needle upward, slowly penetrating through the cornea epithelium to enter a target position of a matrix layer, and finally obtaining the HSV-1 virus suspension. The needle head is tightly attached to the cornea plane in the injection process; after injection is completed, the needle is slowly withdrawn; in the needle inserting and withdrawing processes, real-time observation is carried out through a stereoscopic microscope to ensure that the needle tip is kept away from the crystalline lens. According to the method, the stable and reliable HSK model can be established under the low virus titer (1 * 10 < 5 > PFU), meanwhile, experimental interference caused by mechanical damage is avoided, the specificity and repeatability of the model are improved, and a higher-quality experimental platform is provided for HSK pathogenesis research and drug screening.

Disclosed is the use of a mesencephalic astrocyte-derived neurotrophic factor (Manf) in the preparation of a drug for treating herpes simplex keratitis (HSK). Manf has an anti-herpes simplex virus type I (HSV-1) effect and can inhibit corneal vascular proliferation, corneal inflammatory response, and corneal nerve regression caused by HSV-1 infection. Manf can be used to prepare drugs for treating the HSK.