38 results about "Keratitis" patented technology

The invention relates to eye drops containing exosomes and a preparation method and application thereof, and belongs to the technical field of eye drop preparation, the eye drops comprise exosome freeze-dried powder, the exosome freeze-dried powder is prepared from the exosomes obtained after mesenchymal stem cells are treated with TNF-alpha and IFN-alpha, and a herba houttuyniae extract, the eye drops provided by the invention can inhibit the phenotype of macrophage M1 and promote the M1 to be converted into the phenotype of M2; human corneal cell proliferation, migration and damage repair are promoted; mouse cornea injury repair is accelerated; the eye drops can be used for treating xerophthalmia, conjunctivitis and keratitis, and can also be used for preventing eye diseases and relieving asthenopia; the eye drops disclosed by the invention contain the exosome obtained after the mesenchymal stem cells are treated by the TNF-alpha and the IFN-alpha, so that the anti-inflammatory ability is stronger; the mesenchymal stem cell exosome and the herba houttuyniae extract are stored in a freeze-dried form, so that the activity is stable, and the storage time is long; in addition, the cordate houttuynia is cooperated with the exosome, so that the effect playing time can be prolonged, and the use frequency is reduced.

The invention belongs to the technical field of microorganisms, and particularly relates to bifidobacterium animalis for relieving eye allergy and application of the bifidobacterium animalis. The invention provides an application of bifidobacterium animalis subsp. Lactis in preparation of a product for relieving eye allergy, the bifidobacterium animalis subsp. Lactis is Def +, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.35798. The Bifidobacterium animalis subsp. Lactis Def < + > provided by the invention can relieve the eye itching symptom, relieve red and swollen eyelids, reduce conjunctiva and cornea injury, relieve eye discomfort, relieve globular conjunctivitis cell infiltration, improve corneal epithelium thickening lesion, improve the anti-inflammatory ability and improve allergic conjunctivitis, keratitis and blepharitis marginata in a targeted manner. The eye drops are high in safety and resistant to gastrointestinal fluid, and a safe and long-acting scheme is provided for eye allergic diseases.

The application discloses an ophthalmic disease image classification system based on a big data model, which comprises a data set construction module, an image segmentation module and a model training module.The data set construction module is used for collecting eye images of patients with different ophthalmic diseases in an ophthalmic clinic and performing image marking processing to construct training and test data sets.The image segmentation module adopts YOLOv7 to perform image segmentation on the images taken by the smart phone to obtain eye images containing only the part below the eyebrows and above the zygomatic arch.The model training module adopts a five-fold cross-validation method to train the model on the images, and applies data enhancement technology, white balance adjustment and a transfer learning algorithm.The application relates to the technical field of ophthalmic disease image classification.The ophthalmic disease image classification system based on the big data model has high classification accuracy for diseases such as cataracts, keratitis and pterygium in the development stage test set and different clinical evaluation stages.

The utility model discloses a portable eyeshade capable of preventing exposed keratitis, which comprises a main body unit and a treatment unit, the main body unit comprises an eyeshade body, an elastic band is fixed on the eyeshade body, two through grooves are arranged in the eyeshade body, and the eyeshade body is provided with an adjusting component for adjusting whether shading is carried out or not. The eyeshade comprises two vaseline moisturizing dressings fixed on an eyeshade body, and sterile hydrophilic dressings are fixed on one sides of the vaseline moisturizing dressings. The medicine bag is placed in the storage cavities in the vaseline moisturizing dressing and the sterile hydrophilic dressing, then the vaseline moisturizing dressing is turned over so that the vaseline moisturizing dressing can block the through groove, the medicine bag can treat the eye, the vaseline moisturizing dressing is fixed by embedding the positioning column into the cylinder, namely, the medicine bag is fixed, and the medicine bag can be used for treating the eye. After the shifting rod moves upwards to enable the positioning column to move out of the cylinder body, the vaseline moisturizing dressing can be turned over from the through groove, and the eyes of the patient can be observed through the through groove.

This invention discloses the application of ferrous ions in the preparation of products for treating bacterial infections, belonging to the field of pharmaceutical technology. Antibacterial activity tests of ferrous ions against Staphylococcus aureus showed strong bactericidal effects, with a survival rate of less than 0.001% against Staphylococcus aureus and less than 0.01% against methicillin-resistant Staphylococcus aureus (MRSA). The use of ferrous ion-containing hydrogels in the treatment of keratitis and skin wound infections significantly reduced the risk of MRSA infection in the lungs and effectively prevented the proliferation and spread of microorganisms in the body, revealing that drugs containing ferrous compounds can be used to treat Staphylococcus aureus infections, including MRSA.

The invention discloses an ophthalmology image classification method and device based on global local feature extraction, equipment and a medium, and relates to the technical field of medical image analysis, and the method comprises the steps: obtaining a to-be-classified high-resolution cornea image; inputting the to-be-classified high-resolution cornea image into a preset keratitis image classification model so as to perform fungal image classification on the to-be-classified high-resolution cornea image through the preset keratitis image classification model, and outputting a corresponding aspergillus keratitis image classification result or fusarium keratitis image classification result; wherein the preset keratitis image classification model is an image classification model which learns multi-scale fungus feature extraction capability, fuses image global features and image local features and performs image classification. And fine classification of the two types of fungi is realized through the model.

This invention belongs to the field of molecular diagnostics, specifically relating to biomarkers for the diagnosis of herpes simplex keratitis (HSK) and their applications. This invention is the first to demonstrate that C1QA and FCERT1G are specific and reliable inflammation-related biomarkers for HSK. Diagnostic and assessment methods based on these biomarkers can provide crucial molecular evidence for the early detection, accurate diagnosis, objective severity grading, and personalized treatment of HSK. Simultaneously, this invention reveals the potential roles of FCERT1G and C1QA in HSK and provides new ideas for targeted therapy of this disease, particularly the application of goserelin as an inflammation modulator, offering a new direction for HSK treatment.

The invention provides an acanthamoeba protozoa nano antibody, which is characterized in that the acanthamoeba protozoa nano antibody comprises complementary determining regions CDR1, CDR2 and CDR3, the amino acid sequence of the CDR1 is as shown in SEQ ID NO.1, the amino acid sequence of the CDR2 is as shown in SEQ ID NO.2, and the amino acid sequence of the CDR3 is as shown in SEQ ID NO.3. The nano antibody aiming at the acanthamoeba protozoa is obtained by screening on the basis of an alpaca natural antibody library, and the nano antibody is excellent in solubility, stability, resistance to aggregation, refolding performance and expression yield, low in expression cost and capable of being widely applied and popularized to pathogen detection of tap water, swimming pools, contact lens boxes and clinical patient specimens. Meanwhile, a targeted drug developed based on the antibody makes up the blank of treatment of amebic keratitis at present, and has important clinical value and market potential.

The invention provides a training method of a keratitis grading model fusing focus and complication image blocks, and relates to the field of artificial intelligence, and the method comprises the steps: collecting a plurality of slit lamp image samples; for each slit lamp image sample, identifying a cornea region and a conjunctiva region in the slit lamp image sample by using an image positioning algorithm; randomly cutting at least one first image block in each of upper, lower, left and right boundary areas of the conjunctiva area; randomly cutting at least one second image block in the cornea area; inputting the first image blocks and the second image blocks into a DenseNet121 model in sequence to obtain image features of the image blocks; cascading the image features to obtain a first cascading feature; performing dimension reduction on the first cascade feature by using a PCA algorithm to obtain a first dimension reduction feature; and performing iterative training on the cornea classifier based on the first dimension reduction feature until the cornea classifier converges. According to the method, the performance of the keratitis grading model and the keratitis grading accuracy are improved.

The present invention discloses a preparation method and application of a metal ion coordination gel, wherein the gel material comprises: H-(RADA)4 and (RADA)4 gel stock solution, through histidine (His) and Cu 2+ The resulting complex solution, H-(RADA)4-Cu, is then mixed evenly with the (RADA)4 solution and added to a PBS buffer solution to form a stable gel. This gel exhibits excellent biocompatibility, promotes corneal epithelial cell repair, inhibits the growth of Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli), and is effective in treating keratitis.

The present disclosure provides an ophthalmic formulation comprising: one or more pharmaceutically acceptable excipients; a pharmaceutically active compound capable of reducing the amount or deleterious effects of autoantibodies on the ocular surface, such as IgG; in particular, the present disclosure provides an ophthalmic formulation wherein the pharmaceutically active compound is capable of treating a clinical condition selected from the group consisting of inflammatory, infectious and immunological ocular surface or intraocular diseases that can cause symptoms of ocular discomfort, keratitis, dry eye disease, formation of symblepharon, shortening of the anterior chamber depth, lid margin / conjunctival keratinization, subconjunctival fibrosis, retinal gliosis and glaucoma.

According to the system and method for detecting corneal pathogens, in the system and method, the adsorption capacity is enhanced through ionic liquid modified MXene, silk fibroin in silk fibroin composite hydrogel has good biocompatibility, pathogens on the surface of the cornea can be accurately extracted after compounding, the bacterial adsorption efficiency reaches 92.3 + / -2.7%, and the bacterial adsorption efficiency reaches 92.3 + / -2.7%. The adsorption efficiency of fungi reaches 85.4 + / -3.8%, the adsorption efficiency of viruses reaches 88.6 + / -3.5%, high adsorption efficiency is achieved, and non-invasive sampling is achieved. The combined use of the micro-fluidic chip and the LAMP technology can accurately identify the nucleic acid sequence of a target pathogen, the detected pathogen can be judged only according to the detected fluorescence signal or color change, the pathogen detection can be rapidly completed within 1 h, the detection sensitivity reaches 101-102 CFU / mL, high-sensitivity, rapid and non-invasive detection is realized, and the detection cost is reduced. The kit is suitable for early diagnosis of keratitis.

The present invention discloses the use of ferrous ions in the preparation of products for treating bacterial infections, belonging to the field of medical technology. Ferrous ions were used in antibacterial activity tests on Staphylococcus aureus, demonstrating a strong bactericidal effect. When sterilizing Staphylococcus aureus, the survival rate was less than 0.001%, and when sterilizing methicillin-resistant Staphylococcus aureus (MRSA), the survival rate was less than 0.01%. The use of ferrous ion-containing hydrogels in the treatment of keratitis and skin wound infections significantly reduced the risk of MRSA lung infection and effectively and timely prevented the reproduction and spread of microorganisms in the body. This indicates that drugs containing ferrous compounds can be used to treat Staphylococcus aureus infections, including MRSA.

The present invention relates to a method for classifying keratitis activity using artificial intelligence, comprising: an image receiving step of receiving an anterior segment image of a subject; a preprocessing step of obtaining a pupil region image from the received anterior segment image; and a training step of training an artificial intelligence model using the pupil region image labeled with the activity level of keratitis.

The invention provides contact lens lubricating liquid containing lysozyme and recombinant collagen as well as a preparation method and application of the contact lens lubricating liquid, and belongs to the technical field of eye care products. The contact lens lubricating liquid provided by the invention comprises the following components: 0.01%-0.5% of lysozyme, 0.1%-2.0% of recombinant collagen, 0.5%-1.2% of an osmotic pressure regulator, 0.01%-0.1% of a viscosity regulator and 0.1%-2.0% of a pH buffer agent. The lysozyme and the recombinant collagen are taken as core active components, and the lysozyme and the recombinant collagen have a synergistic effect, so that the gel has the functions of resisting bacteria, preserving moisture, repairing cornea and improving wearing comfort. The invention further provides a preparation method of the contact lens lubricating liquid and application of the contact lens lubricating liquid in preparation of products for relieving xerophthalmia and preventing contact lens related keratitis.

The invention discloses a steam eye patch and a preparation method thereof, the steam eye patch comprises an eye patch and a traditional Chinese medicine matrix liquid, the traditional Chinese medicine matrix liquid comprises the following components in parts by mass: 20-50 parts of wild chrysanthemum, 20-50 parts of golden cypress, 10-50 parts of coptis chinensis, 1-5 parts of salvia miltiorrhiza, 1-5 parts of ginseng, 1-5 parts of dendrobe, 0.00001-1 part of natural borneol, 0.00001-1 part of menthol and 0.5-2 parts of xanthan gum. The eye pad prepared by the invention combines the dual effects of physical hot compress and traditional Chinese medicine components, and various experiments prove that the eye pad can relieve meibomian gland atrophy and blockage, relieve conjunctivitis, keratitis and other inflammatory reactions, improve tear secretion and reduce xerophthalmia, and is very suitable for eye care.

The present invention discloses a pH response contact lens and a preparation mold thereof, and relates to the technical field of contact lens preparation, the pH response contact lens comprises a drug storage layer lens and a covering layer lens, the covering layer lens covers the drug storage layer lens and is located on the inner side of the drug storage layer lens, the covering layer lens is automatically dissolved or degraded when the pH value of tear is higher than a specific threshold value, and at the moment, liquid medicine stored in the medicine storage layer lens flows out. Through mutual cooperation of the medicine storage layer lenses and the covering layer lenses, the covering layer lenses are automatically dissolved when the eyes of a human body are inflamed and the pH value is higher than a normal range, liquid medicine stored in the medicine storage layer lenses automatically flows out, and the precise treatment function of keratitis is achieved.

The invention belongs to the technical field of medical instruments, and particularly discloses a keratitis tear detection kit which comprises a shell, the shell is of a cylindrical structure with an opening in the lower end, through grooves are symmetrically formed in the outer wall of the shell, a center shaft is slidably arranged at the center of the upper end of the shell in a penetrating mode, and a downward-pressing pushing assembly is arranged on the lower wall of the center shaft and the lower wall of the shell. The upper end of the center shaft and the upper wall of the shell are provided with pressing assemblies, the through groove of the shell is provided with an eyelid opening assembly, the lower end of the center shaft and the lower portion of the inner wall of the shell are provided with an eye anastomosis mechanism, the eye anastomosis mechanism comprises an anastomosis linkage assembly, an eye anastomosis head and detection test paper, and the eye anastomosis head is arranged at the lower end of the center shaft. The anastomosis linkage assembly is arranged on the inner wall of the eye anastomosis head and the inner wall of the shell, and the detection test paper is installed on the eye anastomosis head. By pressing the pressing head downwards, the upper eyelids and the lower eyelids of a patient can be kept in an open state, meanwhile, the detection test paper is matched with the eyeballs of the patient in shape, and collection and detection of tears are better completed.

This invention provides a contact lens lubricant containing lysozyme and recombinant collagen, its preparation method, and its application, belonging to the technical field of eye care products. The contact lens lubricant provided by this invention comprises the following components: 0.01%–0.5% lysozyme, 0.1%–2.0% recombinant collagen, 0.5%–1.2% osmotic pressure regulator, 0.01%–0.1% viscosity regulator, and 0.1%–2.0% pH buffer. This invention uses lysozyme and recombinant collagen as the core active ingredients; their synergistic effect provides antibacterial, moisturizing, corneal repair, and improved wearing comfort functions. This invention also provides a method for preparing the above-mentioned contact lens lubricant and its application in the preparation of products for relieving dry eye syndrome and preventing contact lens-related keratitis.

The application relates to the field of ophthalmic drugs, and particularly discloses an eye drop, which comprises the following components in mass fraction: 0.003%-0.2% of chlorhexidine gluconate; and 0.025%-0.05% of chloramphenicol. Since acanthamoeba usually feeds on bacteria, the chlorhexidine gluconate is used as a main component to kill acanthamoeba in the application, and the antibiotic chloramphenicol is added, so that the keratitis caused by bacterial infection can be avoided, the ocular surface bacteria can be killed, the nutrition source of acanthamoeba can be reduced, and the treatment of acanthamoeba keratitis can be synergistically strengthened.

The present invention relates to a stable formulation based on polyhexamethylene biguanide (PHMB) suitable for ocular level administration, a process for its preparation and a relative dosage regimen effective in the treatment of Acanthamoeba keratitis and in particular in the eradication of cysts.

The invention discloses a construction method and system of a herpes simplex keratitis infection model. The method comprises the following steps: fixing a mouse in a mild anesthesia state and enabling eyeballs of the mouse to protrude as much as possible, sucking an HSV-1 virus suspension by using a microsyringe, obliquely inserting a needle from the cornea edge close to the sclera, keeping the inclined surface of the needle upward, slowly penetrating through the cornea epithelium to enter a target position of a matrix layer, and finally obtaining the HSV-1 virus suspension. The needle head is tightly attached to the cornea plane in the injection process; after injection is completed, the needle is slowly withdrawn; in the needle inserting and withdrawing processes, real-time observation is carried out through a stereoscopic microscope to ensure that the needle tip is kept away from the crystalline lens. According to the method, the stable and reliable HSK model can be established under the low virus titer (1 * 10 < 5 > PFU), meanwhile, experimental interference caused by mechanical damage is avoided, the specificity and repeatability of the model are improved, and a higher-quality experimental platform is provided for HSK pathogenesis research and drug screening.

The invention relates to the technical field of keratitis detection systems, in particular to a detection system for rapidly diagnosing keratitis. The invention provides a detection system for rapidly diagnosing keratitis, which comprises a kit and an excitation light source, the kit comprises three independently packaged reagent single tubes; the three reagent single tubes comprise a first reagent single tube, a second reagent single tube and a third reagent single tube which are arranged in sequence according to a medication sequence; a reagent in the third reagent single tube comprises 6PD-DPAN; reagents in the first reagent single tube and the second reagent single tube are independently DO-DPAS or M1-DPAN, and the reagents in the first reagent single tube and the second reagent single tube are different; and the lambda ex of the excitation light source is equal to 200 to 420 nm. The detection system can simply, conveniently, quickly and accurately detect the infection type of keratitis.

The invention discloses an ophthalmology image classification method and device based on contrast loss learning. The method comprises the following steps: acquiring a cornea image to be classified; inputting the cornea image to be classified into the classification model to obtain a classification result of the cornea image to be classified; the classification result at least comprises fusarium keratitis and aspergillus keratitis; the classification model is obtained by training based on a contrast loss function and a sample cornea image pair; the sample cornea image pair is extracted from a clean sample image in advance; the clean sample image is obtained by performing noise processing on the sample image in advance. And similar samples are pulled close and different samples are pushed away by comparing a loss function, so that the distance between the similar samples is reduced, and the model is helped to learn the relationship between the samples. Therefore, the network can more accurately pay attention to the focus area, so that the classification accuracy is improved.

An ophthalmic liposomal product for use in a curative or preventive treatment of disorders or diseases of the eye and periocular area, comprises, or consists of, S75 Phospholipids, lactoferrin, Vitamin E TPGS, Stearylamine, EDTA and a viscosifier selected from the Poloxamer 407 / 188 family, is sterilizable by filtration at 0.2, is stable to aging, with pH between 7.0 and 7.5 and is provided with antibacterial activity which falls under efficacy criteria A, as prescribed by the European Pharmacopoeia F.I.XII. The lactoferrin liposomes with an amount of Stearylamine not exceeding 0.0030% of the total weight and 0.07-0.15% of Vitamin E TPGS % are obtained by extrusion at a pressure between 700 and 800 bar. The lactoferrin liposomes are present at 0.5% by weight on the total weight and the ratio between S75 phospholipids and lactoferrin is 1.5 / 0.5 = 3, sufficient for the lactoferrin to be totally encapsulated. The liposomes of lactoferrin, Stearylamine and Vitamin E TPGS have an electrical potential, measured in distilled water, using a particle analyzer by means of a light scattering technique (DLS), less than 0 mV so as to synergistically strengthen the abilities thereof in preventing and / or treating eye disorders, in particular those in which there is an inflammatory condition such as dry eye syndrome and the related complications, such as chalazion, stye (chronic chalazion), conjunctivitis, blepharitis and keratitis and reducing the possible aging of the lactoferrin protein.

The present disclosure provides an ophthalmic formulation comprising one or more pharmaceutically acceptable excipients; a pharmaceutically active compound that is capable of reducing the amount or deleterious actions of autoantibodies on the ocular surface, such as IgG; In particular, the present disclosure provides an ophthalmic formulation where the pharmaceutically active compound is capable of treating a clinical condition selected from the group consisting of inflammatory, infectious and immunological ocular surface or intraocular disease that can cause symptoms of ocular discomfort, keratitis, dry eye disease, symblepheron formation, fornix foreshortening, eyelid margin / conjunctival keratinization, subconjunctival fibrosis, retinal gliosis and glaucoma.

The invention discloses an AI-based eye postoperative nursing method and system, and relates to the technical field of intelligent medical treatment, and the method comprises the steps: obtaining eye postoperative image data of a patient; analyzing an oculentum covering state of the ocular surface by using an artificial intelligence model and identifying an oculentum loss position; according to the loss position of the oculentum, the multi-point oculentum supplementing mechanism is automatically controlled to carry out accurate dispensing; according to the eyelash density, the trichiasis condition and the eyelid shape of a patient, the opening and closing states of the air holes are automatically adjusted, and sodium hyaluronate atomization is started for moisturizing; a patient is supported to preset a moisturizing mode, and a moisturizing enhancement program matched with the current eye environment is automatically executed when it is recognized that the oculentum is lost. Eye ointment loss is accurately recognized through near-infrared imaging and AI segmentation, supplementary application is conducted according to needs, the moisturizing environment is dynamically adjusted in combination with individualized anatomical characteristics, the liquid medicine deposition efficiency is remarkably improved, and dissipation is reduced; and a preset mode and a time-limited enhanced reinspection mechanism are combined, so that the exposure keratitis risk is remarkably reduced.

The invention provides a composition containing neural stem cells, which comprises the following components in parts by mass: 30-50 parts of neural stem cell suspension, each milliliter of which contains 1.5 * 10 < 6 > to 4.5 * 10 < 6 > neural stem cells; 5 to 10 parts by mass of trehalose-citrate derivative freeze-dried powder; 8-15 parts by mass of a carboxymethyl chemical fibronectin fragment buffer solution; 10-20 parts by mass of a hyaluronic acid-dopamine copolymer aqueous solution; and 0.5-1 part by mass of corneal nerve repairing polypeptide. All the components in the composition disclosed by the invention construct a bionic microenvironment through a synergistic effect. The pH adjusting function of the trehalose-citrate derivative, the physical barrier effect of the hyaluronic acid-dopamine copolymer and the biological guiding function of the carboxymethyl chemical fibronectin fragment jointly maintain the active state of the neural stem cells, and the survival rate and function expression of the neural stem cells in the corneal nerve repairing process are improved.

The application discloses an ophthalmic disease image classification system based on a big data model, which comprises a data set construction module, an image segmentation module and a model training module.The data set construction module is used for collecting eye images of patients with different ophthalmic diseases in an ophthalmic clinic and performing image marking processing to construct training and test data sets.The image segmentation module adopts YOLOv7 to perform image segmentation on the images taken by the smart phone to obtain eye images containing only the part below the eyebrows and above the zygomatic arch.The model training module adopts a five-fold cross-validation method to train the model on the images, and applies data enhancement technology, white balance adjustment and a transfer learning algorithm.The application relates to the technical field of ophthalmic disease image classification.The ophthalmic disease image classification system based on the big data model has high classification accuracy for diseases such as cataracts, keratitis and pterygium in the development stage test set and different clinical evaluation stages.

The invention relates to a drug-resistant bacterium resisting contact lens for treating keratitis as well as a preparation method and application thereof. The drug-resistant bacterium resisting contact lens is made of poly (hydroxyethyl methacrylate); the drug-polymer film is packaged in the drug-resistant bacterium resisting contact lens, or the drug-polymer film is attached to the concave surface of the drug-resistant bacterium resisting contact lens; the drug-polymer film is prepared from the following raw materials: ethyl cellulose, dibutyl sebacate and an antibacterial drug. The invention provides a contact lens delivery system capable of slowly and continuously releasing medicines, which can effectively make up for the defect that the traditional administration method cannot guarantee the continuous treatment effect, and has higher bioavailability.