466 results about "Staphylococcus bacteria" patented technology

The invention discloses an antibacterial peptide based on D-type amino acid and application of the antibacterial peptide, and the antibacterial peptide is characterized in that the amino acid sequence is w-r-r-w-r-r-w-w-r-k-r (dTrp-dArg-dArg-dTrp-dArg-dArg-dTrp-dTrp-dArg-dLys-dArg). The antibacterial peptide can be synthesized through an Fmoc solid-phase chemical method, the synthesis difficulty is low, and the in-vivo antibacterial activity is good. The antibacterial peptide especially has broad-spectrum killing activity on carbapenem-resistant pseudomonas aeruginosa, pseudomonas aeruginosa, klebsiella pneumoniae, escherichia coli, methicillin-resistant staphylococcus aureus and staphylococcus aureus, is low in hemolytic toxicity, can reach half or more of cell survival rate under medium and low concentration, almost has no toxic effect on cells, and can be used for preparing the antibacterial peptide with a broad-spectrum killing activity on carbapenem-resistant pseudomonas aeruginosa, klebsiella pneumoniae, escherichia coli, methicillin-resistant staphylococcus aureus and staphylococcus aureus. The stability is good, the operability is high, and the cost is low.

The invention discloses a staphylococcus aureus biosensor based on a nucleic acid aptamer and a preparation method of the staphylococcus aureus biosensor. Three fluorine atoms (F) are introduced into the 5'end, the 3 'end and the middle position of an aptamer 2' F3-(5 '3'm DNA) sequence (SEQ ID NO: 1) for modification, so that the binding force of the aptamer and graphene is remarkably enhanced, the signal stability and the anti-interference capability of the biosensor are remarkably improved, non-specific fluorescence leakage can be effectively blocked, and reliable guarantee is provided for complex sample detection. According to the present invention, with the cooperation of the graphene oxide quenching substrate, the high-sensitivity detection of the staphylococcus aureus is achieved, the detection limit of the sensor within 5 min can achieve 5 cells / mL, the sensitivity is high, the specificity on the complex sample is more than 95.4%, and the sensor is suitable for the food safety rapid screening and the clinical diagnosis.

The invention belongs to the technical field of antibacterial peptides, and particularly relates to an LL-37 derived antibacterial peptide and application thereof. According to the invention, 17-29aa of the LL-37 antibacterial peptide is used as a core fragment and is connected with an amino acid sequence shown as SEQ ID NO: 1 or SEQ ID NO: 2 through an amido bond, the antibacterial activity of the obtained antibacterial peptide is obviously higher than that of the LL-37 antibacterial peptide, and the antibacterial peptide shows an obvious bactericidal effect on clinical drug-resistant bacteria such as acinetobacter baumannii, pseudomonas aeruginosa, escherichia coli, staphylococcus aureus, klebsiella pneumoniae and the like; meanwhile, the cytotoxicity and hemolytic activity are weak, drug resistance is not prone to being generated, bacteria can be rapidly killed in vivo through multiple mechanisms, no obvious cytotoxicity exists, the bacterium load in tissue is remarkably reduced, and the lifetime of a bacterium-infected mouse is prolonged.

Method and kit for topical treatment of a skin infection, in particular of superficial skin infections, caused by Staphylococcus aureus. The method comprises the steps of applying a photosensitizer to at least a part of the skin affected by the infection, and subjecting said part of the skin to first photons with a majority energy between 2.8 eV and 3.5 eV at a first energy level; and second photons with a majority energy between 1.24 eV and 1.65 eV at a second energy level, the ratio between the first and the second energy levels being in the range from about 10:1 to 2:1. By the method efficient treatment of Staphylococcus aureus infections can be achieved without the administration of topical antibiotics.

The invention relates to the technical field of PA resin, and particularly discloses glass fiber modified antibacterial PA resin suitable for children products and a preparation method of the antibacterial PA resin. The invention discloses glass fiber modified antibacterial PA resin suitable for children products. The glass fiber modified antibacterial PA resin comprises PA resin, glass fibers, talcum powder, nano calcium carbonate, a composite antibacterial agent, a compatilizer, an antioxidant and calcium stearate, the composite antibacterial agent comprises chitosan, an antibacterial peptide microcapsule and a porous silver-zinc-loaded antibacterial agent; the antibacterial peptide microcapsules are sodium alginate embedded antibacterial peptides. The antibacterial PA resin obtained in the invention has the highest bacteriostasis rates of 99.97%, 99.74% and 99.95% for staphylococcus aureus, escherichia coli and candida albicans respectively, the antibacterial property of the PA resin is improved, the compressive strength and notch impact strength are 170.6 MPa and 17.3 kJ / m < 2 > respectively, and high mechanical strength is maintained on the basis of high antibacterial property.

The invention relates to an enzyme response antibacterial carbon dot and a preparation method and application thereof.The carbon dot takes a copper-doped carbon dot as a core and is covalently grafted with a hyaluronic acid shell layer through amidation reaction to form a composite nano material Cu-CDs (at) HA, specifically, soluble copper salt and folic acid are subjected to a hydrothermal reaction to obtain the copper-doped carbon dot, carboxyl is activated through EDC / NHS in a buffer system, and the carbon dot is prepared. And performing grafting reaction with hyaluronic acid to obtain a target product. The hyaluronidase-containing antibacterial composition can be specifically degraded and release antibacterial components under the action of hyaluronidase, shows good bacterial responsive bactericidal performance, has the minimum inhibitory concentrations of 8 g / mL and 16 g / mL for staphylococcus aureus and escherichia coli respectively, and has peroxidase-like activity and free radical scavenging capacity. The antibacterial carbon dots can be used for preparing intelligent wound dressings, antibacterial coatings and other biomedical materials, and have the advantages of responsive drug release, efficient antibacterial property and promotion of wound repair.

The invention belongs to the technical field of functional textile materials, and discloses a modified spandex-based high-elastic antibacterial knitted fabric and a preparation method thereof.The fabric is of a double-layer composite knitted structure and comprises an inner high-elastic breathable layer and an outer antibacterial compact layer; the inner layer is formed by blending modified spandex yarns and modified viscose fibers, and the outer layer is formed by blending modified spandex yarns and superfine polyester fibers. The modified spandex yarn is prepared by introducing a hydrophilic antibacterial monomer compound into a spandex base material and matching with a cross-linking agent for graft modification, the hydrophilic antibacterial monomer compound is formed by compounding guanidyl acrylate and acryloyloxy eugenol, and stable fixation of antibacterial groups is achieved from the molecular level. Meanwhile, the molecular chain structure is regulated and controlled to keep high elasticity. The elongation at break of the fabric is greater than or equal to 300%, the elastic recovery rate is greater than or equal to 95%, and the antibacterial rate on escherichia coli and staphylococcus aureus is greater than or equal to 99%.

The invention discloses a production method of a cool-feeling antibacterial PA66 chinlon filament, and belongs to the technical field of chemical fiber manufacturing. According to the method, PA66 slices are used as raw materials and matched with jade cool-feeling master batches and a septol antibacterial oil agent (the effective component is OPHB), and the PA66 polyamide filament with the cool-feeling antibacterial effect is prepared through the process steps of slice and master batch mixing, melt spinning, side blowing cooling, oiling, drafting and winding and the like. The cool-feeling antibacterial PA66 chinlon filament produced by the invention not only has excellent physical properties (the fineness is 40-50 dtex, the breaking strength is greater than or equal to 3.5 cN / dtex, and the elongation at break is 36-46%), but also has long-acting antibacterial property (the antibacterial rate to escherichia coli and staphylococcus aureus is greater than or equal to 99%, and the antibacterial rate after 50 times of washing is still greater than or equal to 98%) and stable cool feeling (the cool feeling value Q-max is greater than or equal to 0.2 W / (m.K)), and is safe, non-toxic, environment-friendly, simple in process and low in cost. The method is suitable for the field of close-fitting textiles such as warp knitting, weft knitting and hosiery knitting, and the industrial applicability is high.

Provided herein are methods and systems for use in determining risk of a Staphylococcus infection, predicting efficacy of a Staphylococcus vaccine, and prognosing clinical outcomes on a subject exposed to Staphylococcus. Methods of treating subject identified as having or at risk of a Staphylococcus infection are further provided.

The present invention relates to the use of a combination of a saccharide and glycerol for skin protection against undesirable bacteria. The present invention is particularly useful in the formulation of compositions that act as prebiotics for commensal skin bacteria such as S. epidermidis to produce metabolites such as lactic acid, which, through the present invention, has been shown to inhibit the growth of undesirable bacteria such as E. coli and S. aureus, among others. The present invention is especially useful because it provides both short-term (i.e., rapid) and long-term (or sustained) protection.

The invention belongs to the technical field of pharmacy, and mainly relates to a traditional Chinese medicine composition ointment for treating bacterial fungal infection and dermatitis and a preparation method thereof. The traditional Chinese medicinal materials such as the sophora flavescens, the blackberry lily, the cortex dictamni, the gallnut, the fructus cnidii, the aloe, the cortex pseudolaricis, the fructus kochiae, the cortex phellodendri, the typhonium giganteum, the radix stemonae, the hedyotis diffusa, the spina gleditsiae and the rhizoma smilacis glabrae which are subjected to biological fermentation can inhibit the growth of fungi and also can relieve itching and relieve swelling; the traditional Chinese medicine has small side effects; ti / ZIF-8 is also added, so that the antibacterial effect is more obvious; the traditional Chinese medicine composition can be used for treating acute and chronic dermatitis, eczema, dermatophytosis, tinea manuum, foot sweat, fungal infection, skin itch, papule, plaque papule, erythroderma and intractable skin stubborn dermatitis caused by various bacteria and fungi, red swelling and itching caused by mosquito bite, and inhibiting candida albicans, staphylococcus aureus and the like. In general, the method has a very wide application prospect in the field.

An active ingredient combination.

The invention belongs to the technical field of microbial protection and functional metal coating preparation, and particularly relates to a preparation method of a metal-based graphene oxide and zirconium oxide composite antibacterial coating on a flexible metal foil through magnetic field assisted jet electrodeposition. According to the method, a metal ion electroplating solution containing graphene oxide and zirconium oxide serves as a raw material on a flexible metal substrate, ferromagnetic particles are introduced to serve as a carrier, one-time forming is carried out under the action of a magnetic field through the scanning jet electrodeposition technology, and efficient fixing and uniform distribution of graphene oxide and zirconium oxide in a metal coating are achieved. The prepared coating is compact in structure and high in binding force, after the coating is in contact culture with escherichia coli and staphylococcus aureus for half an hour, the antibacterial rate reaches 94% or above, the antibacterial rate reaches 97% or above, and the coating has excellent stability and reliability. The method disclosed by the invention is simple and convenient in process and high in adaptability, can form a high-performance antibacterial coating on a flexible substrate, is suitable for the antifouling and anticorrosion fields of public health facilities, marine protection and engineering equipment, and has a wide industrial application prospect.

The invention belongs to the technical field of oral care, and discloses a rhodiola rosea-containing composite antibacterial oral bacteriostatic paste, which is prepared by compounding rhodiola rosea, tea polyphenol and other five-compound antibacterial components with sorbitol and other auxiliary materials according to an accurate proportion. According to the invention, a triple system of'instant sterilization-lasting bacteriostasis-ecological restoration 'is constructed, third-party detection shows that the inhibition rate on oral pathogens such as escherichia coli, staphylococcus aureus and the like reaches 99.9% or above, and multiple effects of resisting bacteria, diminishing inflammation, protecting gingiva, preventing caries and the like are achieved. The problems that an existing product is single in antibacterial effect, narrow in antibacterial spectrum and low in rhodiola rosea utilization rate are solved, no heavy metal is detected, stability is excellent, irritation is low, and the rhodiola rosea antibacterial agent is suitable for long-term use and has remarkable innovativeness and industrial value.

The application provides a kind of zinc oxide nanoparticles / quaternary amine modified polystyrene nanospheres and its preparation method and application.The application prepares carboxyl functionalized PS / PVP nanospheres with high monodispersity by environmentally friendly water dispersion polymerization method;ZnONPs are successfully loaded after complexing zinc ions with the surface PVP pyrrolidone ring of PS / PVP nanospheres and reducing with reducing agent, and the surface of nanospheres is modified with quaternary amine groups by amidation and further quaternary amination of the surface carboxyl groups of PS / PVP nanospheres.The preparation method is simple, and the reaction condition is easy to realize.The material obtained by the method of the application is polystyrene nanosphere surface loaded with zinc oxide nanoparticles and grafted with quaternary amine groups;it is applied to antibacterial, and zinc oxide nanoparticles and quaternary amine groups play a synergistic effect, so that the material shows high antibacterial activity and can effectively inhibit the proliferation of escherichia coli and staphylococcus aureus.

We provide new monoclonal antibody inhibitors of coagulases, staphylocoagulase (“SC”) and von Willebrand factor-binding protein (“vWbp”) for treatment of S. aureus. The monoclonal antibodies are useful in targeting the SC N-terminus of SC and vWbp (respectively) and inhibiting prothrombin activation. The monoclonal antibodies are able to bind to and interfere with, modulate, and / or inhibit the binding interactions between the coagulase protein and its ligan protein prothrombin the activation of prothrombin.

This invention discloses a method for preparing a chitosan antibacterial film. First, berberine aqueous solution and tannic acid aqueous solution are prepared separately. Then, the berberine aqueous solution is added dropwise to the tannic acid aqueous solution to form a mixed solution. The mixed solution is stirred and heated to obtain a suspension. The suspension is freeze-dried to obtain berberine-tannic acid nanoparticles. Next, a chitosan acetic acid aqueous solution is mixed with the berberine-tannic acid nanoparticles, and a film is formed to obtain a chitosan antibacterial film loaded with berberine-tannic acid nanoparticles. The prepared chitosan antibacterial film has low water content and swelling degree, strong ultraviolet shielding ability, high transparency, and strong inhibitory activity against Staphylococcus aureus and Salmonella typhimurium, which can better meet the needs of food preservation.

The application belongs to the technical field of microorganisms and particularly relates to a Bifidobacterium adolescentis beneficial to skin, a derivative of the Bifidobacterium adolescentis and application of the Bifidobacterium adolescentis and the derivative. The derivative of the Bifidobacterium adolescentis has significant antioxidant and anti-inflammatory effects, can inhibit pathogenic bacteria of acne and other common skin infections such as Propionibacterium acnes, Staphylococcus aureus and Malassezia furfur, can eliminate pathogenic bacteria, can inhibit skin lipid accumulation, can avoid follicle blockage, and can improve skin problems in multiple directions, especially acne skin problems.

The application discloses a silver / schiff base antibacterial material, a preparation method and application, and relates to the field of antibacterial materials.The silver / schiff base antibacterial material, the preparation method and the application are characterized in that, based on the large electron cloud density of the carbon-nitrogen double bond of the schiff base, silver ions in a silver nitrate solution are uniformly adsorbed on a schiff base base through the electrostatic adsorption principle; under the reduction of a reducing agent, the silver ions are reduced to nanosilver ions, and a silver / schiff base antibacterial material is obtained, in which nanosilver ions in the form of nanospheres are uniformly dispersed on the schiff base in the form of a thin sheet layer, thereby greatly improving the dispersibility of the nanosilver ions.The preparation method is simple, low in cost, and capable of batch production, and has no cytotoxicity, and has high antibacterial effects on streptococcus, pseudomonas aeruginosa and staphylococcus aureus, and has a good application prospect.

The present invention relates to the field of biological fermentation application technology, and in particular, to use of a milk peptide fermentation product in regulating skin microecological balance. The milk peptide fermentation product selectively regulates the growth of Staphylococcus epidermidis and inhibits the growth of Staphylococcus aureus and Escherichia coli. It was discovered for the first time that the milk peptide fermentation product has a selective bacteriostatic effect on skin microorganisms. The experimental results show that the milk peptide fermentation product has an inhibitory effect on Escherichia coli and Staphylococcus aureus, but has no effect on the normal growth of Staphylococcus epidermidis. This characteristic is obviously different from common broad-spectrum chemical bacteriostatic agents, and the use of the milk peptide fermentation product is more conducive to maintaining the balance of skin microbiota.

A general formula for lysins based on which the activity and functionality are further screened and 24 lysins are identified and further investigated, nine of which are characterized. These lysins exhibit potent bactericidal activity, capable of lysing multiple species of Staphylococcus, Streptococcus, and / or Enterococcus; demonstrate robust environmental resistance and stability, retaining activity under varying temperatures, NaCl concentrations, pH levels, and in immune serum; and effectively treat localized and systemic infections. These lysins and their variants are promising as antimicrobial agents for eradicating bacteria, preventing bacterial infections, or treating bacterial infections (caused by genera Staphylococcus, Streptococcus, and / or Enterococcus).

This invention discloses the application of ferrous ions in the preparation of products for treating bacterial infections, belonging to the field of pharmaceutical technology. Antibacterial activity tests of ferrous ions against Staphylococcus aureus showed strong bactericidal effects, with a survival rate of less than 0.001% against Staphylococcus aureus and less than 0.01% against methicillin-resistant Staphylococcus aureus (MRSA). The use of ferrous ion-containing hydrogels in the treatment of keratitis and skin wound infections significantly reduced the risk of MRSA infection in the lungs and effectively prevented the proliferation and spread of microorganisms in the body, revealing that drugs containing ferrous compounds can be used to treat Staphylococcus aureus infections, including MRSA.

The invention relates to an antibacterial gel and a preparation method thereof. The gel mainly comprises silver ions, avocado oil, a radix puerariae extract, wheat germ oil, emulsifying wax, a folium artemisiae argyi extract, glycerol, a styrene / butadiene copolymer, purified water and hydroxyethyl cellulose. Extracts containing specific small-molecule polypeptides are extracted from Artemisia rubescens and Radix Puerariae through a specific extraction method, and the polypeptides are Artemisia rubescens polypeptides (SEQ ID No: 1) and Radix Puerariae polypeptides (SEQ ID No: 2) respectively. The extracts have a synergistic effect with silver ions, so that the antibacterial effect of the gel is remarkably improved, and particularly, the gel has a remarkable inhibiting effect on staphylococcus aureus, escherichia coli and candida albicans. The bacteriostatic gel is simple in preparation method, convenient to use and suitable for bacteriostasis of female vaginal mucosa and maintenance of vaginal micro-ecological internal environment.

The invention is applicable to the technical field of genetic engineering, and provides a vaccine for preventing and treating staphylococcus aureus mastitis and application of the vaccine. A recombinant bacillus subtilis construction method comprises the following steps: taking a DNA sequence as shown in SEQ ID NO.3 as a template, and performing PCR amplification to obtain a PCR product sequence; connecting the PCR product to a pHT43 vector, and converting the pHT43 vector into a competent cell DH5alpha, so as to obtain a recombinant plasmid pHT43-cCsa; the method comprises the following steps: transforming a recombinant plasmid pHT43-cCsa into a competent cell of bacillus subtilis WB800N, taking pHT43-F and pHT43-R as primers, and screening a positive transformant through a PCR (Polymerase Chain Reaction) technology; the recombinant bacillus subtilis for expressing the staphylococcus aureus Csa protein is constructed by utilizing a homologous recombination technology, so that pathological injury caused by staphylococcus aureus infection can be reduced, and staphylococcus aureus mastitis of dairy cattle can be prevented.

The invention belongs to the technical field of functional materials, and discloses an amidoxime-based chitosan microporous membrane adsorption material as well as a preparation method and application thereof. According to the amidoxime-based chitosan microporous membrane adsorption material, a chitosan microporous membrane is used as a matrix, and amidoxime groups are grafted on the matrix; the adsorption material has a microporous structure, the average pore size is 0.05-0.3 mu m, and the bacteriostasis rate on escherichia coli and staphylococcus aureus is not lower than 90%. The amidoxime group introduced in the invention has specific chelating ability to uranyl ions. Even in a simulated salt lake brine environment in which high-concentration competitive cations exist, the membrane adsorption material can still maintain high-selectivity adsorption on uranium, the extraction rate can reach 86% or above, and extremely high ion interference resistance is shown. The broad-spectrum antibacterial property of the chitosan base material is creatively utilized, so that the antibacterial rates of the obtained membrane adsorption material on escherichia coli and staphylococcus aureus exceed 90%.

The invention discloses a high-strength nano-composite antibacterial fabric which comprises a base fabric and a modified and sol-gel nano layer, the modified and sol-gel nano layer and the base fabric are compounded and smeared to form the nano-composite antibacterial fabric, and the modified and sol-gel nano layer is coated with the modified and sol-gel nano layer to form the high-strength nano-composite antibacterial fabric. The modified sol-gel nano layer comprises titanium dioxide sol, nano zinc oxide and a silver antibacterial agent, the base layer fabric is prepared from the following raw materials in percentage by weight: 10% of chitin fiber, 50% of Coolplus fiber, 40% of cotton fiber and 16.7 tex of yarn linear density, and the base layer fabric is prepared from the following raw materials in percentage by weight: 10% of chitosan fiber, 50% of Coolplus fiber, 40% of cotton fiber and 16.7 tex of yarn linear density. Compared with the prior art, the fabric has the advantages that a layer of uniform and firm porous nano antibacterial film is formed on the surface of the fabric, so that staphylococcus aureus and pathogenic escherichia coli can be effectively killed; the double-layer fabric formed by combining the chitin fibers, the Coolplus fibers and the cotton fibers has good antibacterial, bacteriostatic, rapid moisture absorption and moisture conduction functions.

The application belongs to the field of antibacterial two-dimensional nanomaterial Mxene catalysts, and particularly relates to a monolayer W@MoS2 nanosheet as well as a preparation method and application thereof. The method comprises the following steps: 1) dissolving water-soluble molybdate and water-soluble tungstate in water to obtain a mixed solution; 2) adding a sulfur source and an organic solvent to the mixed solution, and then performing reaction after mixing, and drying to obtain a powder. The monolayer W@MoS2 nanosheet has a unique two-dimensional planar structure, a large specific surface area, and good optical and electronic properties, and has high light-heat absorption in the near-infrared two region, i.e., the light-heat conversion efficiency is improved. Meanwhile, the peroxidase-like activity of the monolayer W@MoS2 nanosheet material realizes efficient light-heat amplification, can treat methicillin-resistant Staphylococcus aureus infection, and avoids heat therapy damage to normal tissues.

The invention discloses an antibacterial hydrogel dressing taking spunlace non-woven fabric as a carrier and a preparation method of the antibacterial hydrogel dressing, and relates to the technical field of biomedical polymers. The antibacterial hydrogel dressing is composed of a spunlace non-woven fabric carrier layer and an antibacterial hydrogel functional layer loaded on the surface and in pores of the spunlace non-woven fabric carrier layer, and the preparation method comprises the following steps: sterilizing spunlace non-woven fabric to obtain a spunlace non-woven fabric carrier; adding the antibacterial component into the aqueous solution of the hydrophilic polymer base material to form a hydrogel precursor solution; and immersing the spunlace non-woven fabric carrier into the hydrogel precursor solution, and curing to obtain the antibacterial hydrogel dressing. The antibacterial hydrogel dressing prepared by the preparation method disclosed by the invention has the flexible breathable characteristic of spunlace non-woven fabric and the high swelling and antibacterial functions of hydrogel, has an excellent bactericidal effect on escherichia coli and staphylococcus aureus, can effectively promote healing of infected wounds, is suitable for clinical nursing of burn and traumatic skin injuries, and has wide application prospects. Wide clinical application prospects are realized.

The application belongs to the field of cosmetics, and particularly relates to a bacillus fermentation product for repairing and moisturizing, and a preparation method and application thereof. The preparation method of the bacillus fermentation product for repairing and moisturizing comprises the following steps: S1, culturing bacillus in a culture medium to obtain bacillus spores; S2, treating the bacillus spores obtained in step S1 with lysozyme and protease, and then treating the bacillus spores with an ultrahigh-pressure homogenizer to obtain a spore lysis product; and S3, centrifuging, concentrating and heating the spore lysis product obtained in step S2 to obtain the bacillus fermentation product for repairing and moisturizing. The spore lysis product is applied to the field of cosmetic raw material preparation, and the bacillus fermentation product obtained by the application has excellent repairing and moisturizing effects, can inhibit staphylococcus aureus, and has the effect of balancing skin microecology.

Described herein is a synergistic combination comprising a quorum-sensing inhibitor and / or postbiotic metabolite and an antibiotic. Typically, the postbiotic metabolite comprises at least one peptide. Related compositions, uses, and methods are also described, including methods for resensitizing resistant bacteria to an antibiotic, and methods of treating antibiotic-resistant infections, such as methicillin-resistant Staphylococcus aureus (MRSA).