1300 results about "Staphylococcus bacteria" patented technology

The invention discloses bacillus velezensis as well as fermentation product filtrate and a biological product thereof, and belongs to the technical field of microorganisms, a bacillus velezensis BTN-HB-F4 strain is separated and purified from prinsepia utilis royle in Harba snow mountain in Yunnan province, the fermentation product filtrate of the bacillus velezensis has excellent HAS inhibition capacity and remarkable DPPH and ABTS free radical scavenging capacity, and the biological product of the bacillus velezensis has the advantages that the biological product can be used for preparing biological products of the prinsepia utilis royle; a good anti-oxidation effect can be realized; secondly, under various concentrations, fermentation product filtrate can remarkably reduce the proportion of SA-beta-Gal positive cells, gene expression of matrix metalloproteinase 3 can be remarkably inhibited, and a good anti-aging effect is achieved; in addition, fermentation product filtrate can remarkably inhibit generation of zebra fish melanin and has a good whitening effect; the secretion amount of cell inflammatory factors IL-6 and TNF-alpha can be remarkably inhibited, and a good relieving effect is achieved; finally, the fermentation product filtrate can effectively inhibit the growth of pathogenic bacteria staphylococcus aureus, and has the potential of regulating the skin micro-ecology.

The invention provides an antibacterial peptide cAMP048 sourced from microorganisms in a deep sea environment and application of the antibacterial peptide cAMP048, and belongs to the technical field of peptide inhibitors. The antibacterial peptide cAMP048 has an amino acid sequence as shown in SEQ ID NO: 1 (Sequence Identity Number 1). The antibacterial peptide can inhibit the growth of acinetobacter baumannii at 64 mu M, inhibit the growth of multi-drug-resistant acinetobacter baumannii and multi-drug-resistant enterococcus faecium at 128 mu M and inhibit the growth of escherichia coli, staphylococcus aureus and multi-drug-resistant klebsiella pneumoniae at 256 mu M. The antibacterial peptide can be used for preparing products with antibacterial and / or bactericidal performance, and can be applied to preparation of antibacterial and / or bactericidal products. Such as food, skin care products or medical supplies.

The invention relates to a composite hydrogel dressing for promoting wound healing as well as preparation and application thereof, and belongs to the technical field of biological medicines. The hydrogel disclosed by the invention is prepared by mixing gelatin, oxidized dextran and metal ion modified metal-organic framework compounds (MOF-Ag and MOF-Cu). In the mixed solution, amino groups in the gelatin and aldehyde groups of the oxidized dextran are subjected to a Schiff base cross-linking reaction, and the composite hydrogel is formed after standing. The loaded MOF-Ag has antibacterial and anti-biofilm effects on staphylococcus aureus and escherichia coli, and the loaded MOF-Cu has similar superoxide dismutase activity and similar glutathione peroxidase activity, so that excessive active oxygen can be removed, and inflammatory response can be relieved. The obtained hydrogel has good biocompatibility, proper mechanical properties and biological adhesion, stable rheological properties, excellent antibacterial and anti-inflammatory functions and a certain hemostatic function.

The invention relates to lactobacillus gasseri FMTA1125B with the effect of preventing, relieving or treating vaginitis and application of the lactobacillus gasseri FMTA1125B. The lactobacillus gasseri FMTA1125B is classified and named as lactobacillus gasseri, and the preservation number of the lactobacillus gasseri FMTA1125B is CGMCC No.34292. The lactobacillus gasseri FMTA1125B has the effects of preventing, relieving or treating vaginitis and the application of the lactobacillus gasseri FMTA1125B. The bacterium can produce beneficial components such as lactic acid, gamma-aminobutyric acid and hydrogen peroxide through metabolism so as to regulate the genital tract environment and improve the health degree; and the traditional Chinese medicine composition has relatively good bacteriostasis and sterilization effects on common vaginal pathogenic bacteria such as escherichia coli, staphylococcus aureus, gardnerella vaginalis, candida albicans, neisseria gonorrhoeae and the like. Therefore, the lactobacillus gasseri FMTA1125B strain can be used for preparing a preparation with the effect of improving related genital tract diseases such as vaginitis.

The invention provides a test strip for rapidly detecting methicillin-resistant staphylococcus aureus and application of the test strip, and belongs to the technical field of biochemical detection. The test strip comprises a bottom plate, a sample pad, a combination pad, a detection pad and a water absorption pad, the two ends of the detection pad are in lap joint with the combination pad and the water absorption pad respectively, the detection pad is located on the lower portion, the sample pad is in lap joint with the combination pad, and the combination pad is located on the lower portion; a detection line and a quality control line are arranged on the detection pad, the detection line is arranged on one side close to the combination pad, and the quality control line is arranged on one side close to the water absorption pad; the detection pad is coated with a MoS2 nanosheet, the combination pad is coated with a penicillin binding protein 2a polyclonal antibody labeled by horse radish peroxidase, and the detection line is coated with a penicillin binding protein 2a monoclonal antibody; the quality control line is coated with goat anti-rabbit IgG (immunoglobulin G). The kit is high in sensitivity and wide in linear range, has good specificity, repeatability and stability, and can effectively detect MRSA in a clinical sample.

The invention discloses an antibacterial peptide and application thereof. The amino acid sequence of the antibacterial peptide disclosed by the invention is KKKEHRLLHRVLFHRL. The minimum inhibitory concentration of the antibacterial peptide to staphylococcus aureus is 4.5 mu g / mL, and the minimum bactericidal concentration of the antibacterial peptide to staphylococcus aureus is 72.7 mu g / mL. The antibacterial peptide disclosed by the invention has remarkable antibacterial activity on staphylococcus aureus and is expected to be widely applied as a novel bacteriostatic agent for preventing and treating staphylococcus aureus.

The invention relates to a nutritional composition. The nutritional composition comprises medium-chain and long-chain fatty acid triglyceride and nervonic acid in a mass ratio of (3: 1)-(1500: 1), and has a synergistic effect in the aspects of preventing or relieving diarrhea, inhibiting staphylococcus aureus in vivo or in vitro and / or preventing or treating inflammation. The invention also relates to a food product comprising said nutritional composition. The invention also relates to application of the nutritional composition in preparation of products with one or more purposes selected from 1) to 3): 1) preventing or relieving diarrhea; 2) inhibiting staphylococcus aureus in vivo or in vitro; and 3) preventing or treating inflammation.

The invention discloses staphylococcus xylosus with a lipid degradation function and application of the staphylococcus xylosus in fermented food, and belongs to the field of microorganisms. The invention provides staphylococcus xylosus S3, the staphylococcus xylosus S3 has the capability of degrading lipid, and when the staphylococcus xylosus S3 is fermented in surimi for 48 hours, the acid value and the TBARS value of the fermented surimi can be effectively improved. Therefore, the staphylococcus xylosus S3 can adjust the lipid hydrolysis degree in the fermented surimi to an ideal range, and has broad application prospects in the food industry.

The invention discloses a Staphylococcus edaphicus H3, the preservation number of the Staphylococcus edaphicus H3 in Guangdong Microbial Culture Collection Center is GDMCC No: 64958, the strain H3 is applied to fermentation of meat products without nitrite, the Staphylococcus edaphicus H3 can promote color development of the meat products, and the Staphylococcus edaphicus H3 has relatively strong oxidation resistance, can effectively inhibit lipid peroxidation, and can be used for preparing the meat products. The staphylococcus aureus H3 can produce polysaccharide, the polysaccharide also plays an anti-oxidation role in a meat product processing process and is beneficial to maintenance of meat color, the strain H3 is inoculated to ferment sausages, the nitrite content in the sausages is obviously reduced, the hardness, cohesiveness, elasticity and chewiness of the sausages are improved, the taste of the sausages is richer, the delicate flavor is increased, and the sausages are more delicious. The strain H3 has no blood coagulation and hemolysis effects, is safe and reliable as a food additive, and is suitable for industrial production and market popularization and application.

The invention belongs to the technical field of medicines, and particularly relates to application of sesquiterpenoids and / or pharmaceutically acceptable salts thereof in preparation of medicines for treating or preventing infectious diseases caused by staphylococcus aureus. The invention relates to the field of medicines, in particular to application in preparation of medicines for treating or preventing infectious diseases caused by drug-sensitive staphylococcus aureus or drug-resistant staphylococcus aureus. The structure of the sesquiterpenoids is shown as a formula I in the specification. The sesquiterpenoids applied in the invention are derived from rattan chrysanthemum, the source is wide, and the production cost is low. The sesquiterpenoids shown in the formula I have a very good activity inhibition effect on staphylococcus aureus for the first time, and the sesquiterpenoids have a very good activity inhibition effect on staphylococcus aureus with drug sensitivity, drug medium or drug resistance.

The invention belongs to the technical field of microorganisms, and particularly relates to a lactobacillus polyactis composition for improving skin as well as a derivative and application of the lactobacillus polyactis composition. The composition comprises a bacterial strain, a pure culture or an inactivated bacterium body of bifidobacterium bifidum Bbifid-1 (CCTCC NO: M2023349), bifidobacterium adolescentis Badol-1 (CCTCC NO: M 20242593), phytobacterium plantarum Lplan-4 (CCTCC NO: M 20242594) and lactobacillus acidophilus (BA05). The invention further relates to a preparation method of the composition. The lactobacillus plantarum derivative composition prepared through treatment modes of splitting decomposition, extraction, fermentation and the like can be used as an active ingredient of a medicine or a cosmetic, has remarkable effects of resisting oxidation and bacteria, especially resisting inflammation and inhibiting skin lipid accumulation aiming at propionibacterium acnes and staphylococcus aureus, is beneficial to fading skin red and swollen, avoids hair follicle blockage, and has a good application prospect. The obvious effect on the acne is realized.

The invention discloses a hydrogel dressing with a double-slow-release effect as well as a preparation method and application of the hydrogel dressing. The preparation method of the hydrogel dressing comprises the following steps: firstly, preparing methyl acrylate modified hyaluronic acid capable of being photo-crosslinked, then preparing carboxymethyl chitosan nanoparticles loaded with chlorogenic acid, and finally, mixing the methyl acrylate modified hyaluronic acid and the carboxymethyl chitosan nanoparticles loaded with chlorogenic acid to obtain the hydrogel dressing. The hydrogel dressing embedded with the nanoparticles is obtained in a photo-crosslinking mode. The hydrogel dressing provided by the invention not only has good biocompatibility and mechanical properties, but also plays an anti-oxidation role by removing free radicals in a body, and can effectively kill bacteria such as escherichia coli and staphylococcus aureus to play an antibacterial role. The hydrogel dressing can also reduce excessive inflammatory response in the wound healing process by influencing macrophage polarization, promote chronic wound healing and relieve pain of patients.

The invention relates to the technical field of hydrogel, in particular to a preparation method and application of EGCG-mediated hydrogel, a lysozyme solution is heated under the acidic condition to form lysozyme amyloid fibrils LAFs, EGCG is dissolved in a buffer solution and mixed with the LAFs solution obtained in the step 1 in proportion to form an LAFs-EGCG compound, and the EGCG-mediated hydrogel is prepared. The chitosan CS is dissolved in an acetic acid buffer solution, glutaraldehyde is added to serve as a cross-linking agent, the mixture is mixed with the LAFs-EGCG compound obtained in the step 2 and stirred, the composite hydrogel is formed, the concentration of EGCG is 0.2-1.0 wt%, the concentration of LAFs is 5 wt%, the concentration of chitosan is 2 wt%, the pH value of the lysozyme solution in the step 1 is 2.0, the heating temperature is 77 DEG C, the heating time is 10 hours, the concentration of EGCG in the step 2 is 0.4-0.8 wt%, and the pH value of the lysozyme solution in the step 2 is 1.5-2.5. In the step 3, the concentration of glutaraldehyde is 2%, the storage modulus G'of the hydrogel is 325-480 Pa, the hardness is 2176.71 g, and the bacteriostasis rate on staphylococcus aureus and salmonella exceeds 95%.

The invention provides a nutritional composition and application of the nutritional composition. The nutritional composition comprises medium-chain and long-chain fatty acid triglyceride (MLCT) and milk fat globule membrane (MFGM) in a mass ratio of 2: 1 to 80: 1, and the combination of the two has the effects of synergistically improving diarrhea, inhibiting staphylococcus aureus in vivo or in vitro and / or regulating intestinal flora.

The invention discloses an antibacterial peptide based on D-type amino acid and application of the antibacterial peptide, and the antibacterial peptide is characterized in that the amino acid sequence is w-r-r-w-r-r-w-w-r-k-r (dTrp-dArg-dArg-dTrp-dArg-dArg-dTrp-dTrp-dArg-dLys-dArg). The antibacterial peptide can be synthesized through an Fmoc solid-phase chemical method, the synthesis difficulty is low, and the in-vivo antibacterial activity is good. The antibacterial peptide especially has broad-spectrum killing activity on carbapenem-resistant pseudomonas aeruginosa, pseudomonas aeruginosa, klebsiella pneumoniae, escherichia coli, methicillin-resistant staphylococcus aureus and staphylococcus aureus, is low in hemolytic toxicity, can reach half or more of cell survival rate under medium and low concentration, almost has no toxic effect on cells, and can be used for preparing the antibacterial peptide with a broad-spectrum killing activity on carbapenem-resistant pseudomonas aeruginosa, klebsiella pneumoniae, escherichia coli, methicillin-resistant staphylococcus aureus and staphylococcus aureus. The stability is good, the operability is high, and the cost is low.

The invention discloses an antibacterial finishing agent containing quaternary ammonium salt chitosan and tannic acid as well as a preparation method and application of the antibacterial finishing agent. The antibacterial finishing agent is mainly a mixed solution containing quaternary ammonium salt chitosan, tannic acid and glacial acetic acid. The preparation method comprises the following steps: dispersing chitosan in deionized water, then adding 2, 3-epoxypropyltrimethylammonium chloride, heating and stirring, reacting, and purifying and drying to obtain quaternary ammonium salt chitosan; and dissolving quaternary ammonium salt chitosan in an acetic acid solution, and adding tannic acid to obtain the antibacterial finishing agent. In the application, the fabric is soaked in the antibacterial finishing agent, then soaked in deionized water for washing and finally dried, the finished and modified fabric is high in antibacterial property, the antibacterial rate on escherichia coli is 99.2%, and the antibacterial rate on staphylococcus aureus is close to 100%.

The invention discloses preparation and application of an anti-staphylococcus aureus IsdB antibody. The antibody comprises a heavy chain variable region and a light chain variable region, the heavy chain variable region comprises HCDR1, HCDR2 and HCDR3 in the heavy chain variable region as shown in SEQ ID NO: 8; the light chain variable region comprises LCDR1, LCDR2 and LCDR3 in the light chain variable region as shown in SEQ ID NO: 16. The invention also provides nucleic acid for coding the antibody, a vector containing the nucleic acid and a cell containing the vector. In addition, experiments prove that the antibody can inhibit systemic infection of MRSA and resist invasion of MRSA to pneumonia, and has a good clinical application prospect.

The invention provides a thiazolidinone compound and application thereof in preparation of drugs for resisting staphylococcus aureus infection. The molecular structural formula of the thiazolidinone compound is shown as a formula (1), a formula (2), a formula (3) or a formula (4). The technical scheme of the invention discloses several thiazolidinone compounds, which not only have an excellent bactericidal effect on staphylococcus aureus and can significantly inhibit the formation of a biofilm of staphylococcus aureus, but also have low cytotoxicity and hemolytic activity. According to the technical scheme, a certain foundation is laid for research and development of novel staphylococcus aureus biofilm infection resisting drugs, and the potential of thiazolidone derivatives for developing novel antibacterial agents for staphylococcus aureus related infection can be deeply known possibly.

The invention relates to a double-probe detection reagent for detecting methicillin-resistant staphylococcus aureus and a preparation method, and belongs to the technical field of food safety. The double-probe detection reagent is composed of a magnetic separation probe reagent solution and a fluorescent nano-enzyme probe reagent solution; the magnetic separation probe reagent liquid is used for specifically binding methicillin-resistant staphylococcus aureus and carrying out separation and enrichment treatment on a detection sample; the blue fluorescent nano-enzyme probe reagent solution is used for specifically combining, separating and enriching the methicillin-resistant staphylococcus aureus and generating a concentration dependent colorimetric signal and a fluorescent signal of the methicillin-resistant staphylococcus aureus. The magnetic separation probe has high specific surface area and super-strong magnetism, the capture efficiency is enhanced, and the magnetic attraction time is shortened; according to the invention, mutual verification is carried out between dual-mode signals, an internal proofreading effect is achieved, and the accuracy of a detection result is improved; the detection time of the method is within 1 h, the detection time is short, the detection sensitivity is high, and the method has good specificity for common type strains.

The invention relates to a norhalal-enoxacin conjugate as well as a preparation method and application thereof. The norhalal-enoxacin conjugate comprises a norhalal-enoxacin coupling compound, or a stereoisomer, a prodrug, a crystal form, a pharmaceutically acceptable salt, a pharmaceutically acceptable ester or a pharmaceutically acceptable solvate selected from the compound. Compared with enoxacin, kanamycin, streptomycin, lincomycin and azithromycin, the norharmann-enoxacin conjugate disclosed by the invention has a smaller MIC (Minimum Inhibitory Concentration) value, which shows that the norharmann-enoxacin conjugate has a better anti-staphylococcus aureus effect.

The invention relates to the technical field of biological medicine, in particular to an antibody aiming at staphylococcus aureus enterotoxin B and application thereof. A heavy chain variable region of the antibody provided by the invention comprises CDR sequences as shown in SEQ ID NO.1-3, and a light chain variable region of the antibody comprises CDR sequences as shown in SEQ ID NO.9-11. The antibody has high affinity, can specifically bind to staphylococcus aureus SEB, can block binding of SEB and MHC II / TCR, significantly inhibits cytokine storm, shows a dose-dependent protection effect in an MRSA systemic infection model, and can be used for preparing an MRSA systemic infection model. The monoclonal antibody can be used for treating, preventing or diagnosing the infection of the staphylococcus aureus, provides a solution of non-antibiotic therapy for SEB poisoning and drug-resistant staphylococcus aureus infection, and has important clinical and public health values.

The invention provides a kit for rapidly detecting MRSA and drug-resistant gene nucleic acid POCT of the MRSA based on a phage lyase mutant. The kit comprises nucleic acid extraction and purification and triple PCR rapid amplification detection reagents. The staphylococcus aureus bacteriophage lyase mutant nucleic acid release liquid containing the amino acid sequence as shown in SEQ ID NO: 1 is adopted, wall breaking can be rapidly conducted on staphylococcus aureus, and nucleic acid is released. The effect of the nucleic acid release liquid acting for 5 minutes is equivalent to or even better than that of the lysozyme acting for 60 minutes; according to the present invention, with the combination of the MRSA and drug-resistant gene triple PCR rapid amplification detection reagent, the total detection time is only 35 min, the time is saved by 55 min or more than 55 min compared with the lysozyme method, and the MRSA and drug-resistant gene nucleic acid POCT rapid detection can be achieved;

The present invention discloses a fermented mucus lactobacillus and its application. The present invention provides a fermented mucus lactobacillus A10 with high extracellular polysaccharide production, and its deposit number is CCTCC NO: M20242082. The fermented mucus lactobacillus A10 has strong bile salt and gastrointestinal digestion tolerance, and has obvious antibacterial effects on Escherichia coli and Staphylococcus aureus. The extracellular polysaccharide synthesized by the fermented mucus lactobacillus A10 is a Wzy type extracellular polysaccharide, which is a heteropolysaccharide composed of rhamnose, galactose, glucose, mannose, and glucuronic acid. It has good thermal stability and antioxidant activity, and can significantly scavenge free radicals. The fermented mucus lactobacillus A10 extracellular polysaccharide provided by the present invention can exert antioxidant activity by activating the Keap1-Nrf2 / ARE signaling pathway, and is expected to be developed into a natural antioxidant that interacts with Keap1-Nrf2 in a targeted manner.

The invention discloses a staphylococcus aureus biosensor based on a nucleic acid aptamer and a preparation method of the staphylococcus aureus biosensor. Three fluorine atoms (F) are introduced into the 5'end, the 3 'end and the middle position of an aptamer 2' F3-(5 '3'm DNA) sequence (SEQ ID NO: 1) for modification, so that the binding force of the aptamer and graphene is remarkably enhanced, the signal stability and the anti-interference capability of the biosensor are remarkably improved, non-specific fluorescence leakage can be effectively blocked, and reliable guarantee is provided for complex sample detection. According to the present invention, with the cooperation of the graphene oxide quenching substrate, the high-sensitivity detection of the staphylococcus aureus is achieved, the detection limit of the sensor within 5 min can achieve 5 cells / mL, the sensitivity is high, the specificity on the complex sample is more than 95.4%, and the sensor is suitable for the food safety rapid screening and the clinical diagnosis.

The invention belongs to the technical field of microorganisms, and discloses a Lactobacillus paracasei B20 for inhibiting melanin from a plateau area and application of the Lactobacillus paracasei B20, the Lactobacillus paracasei B20 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation number is CGMCC No.32434, and the preservation date is October 31, 2024. Metabolite of the lactobacillus paracasei B20 comprises phenylalanine, p-hydroxycinnamic acid and the like, has strong tyrosinase inhibition effect and anti-ultraviolet ability, and can significantly reduce generation of melanin in skin. In addition, the lactobacillus paracasei B20 also has relatively strong oxidation resistance, has good tolerance in artificial gastric juice and artificial intestinal juice, and can smoothly reach the intestinal tract of a human body. In addition, the lactobacillus paracasei B20 has a relatively obvious growth inhibition effect on skin conditioned pathogens such as staphylococcus aureus and enterococcus faecalis.

The invention provides a composition, a kit and a detection method for detecting staphylococcus aureus or a drug-resistant gene thereof based on RPA-CRISPR / Cas12a and application of the composition, the kit and the detection method, and belongs to the technical field of gene detection. A CRISPR / Cas12a system is combined with recombinase polymerase amplification (RPA), an RPA-CRISPR / Cas12a detection method for rapidly detecting the staphylococcus aureus in the wastewater and the drug-resistant gene of the staphylococcus aureus is established, a specific primer and crRNA are designed, the high-specificity nucleic acid cutting capability of a gene editing enzyme CRISPR / CAS system and the isothermal rapid amplification advantage of a nucleic acid isothermal amplification technology are fully fused, and the detection method has the advantages of high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity, high specificity The fluorescent probe has high-specificity sequence recognition capability, realizes efficient detection of target nucleic acid, is strong in detection specificity, high in sensitivity and simple and convenient to operate, does not need complex equipment and professionals, and is suitable for rapid detection in an on-site environment with limited resources.

The invention discloses application of macadamia nut green peel in preparation of an antibacterial agent, and belongs to the technical field of application of antibacterial agents. The invention finds that the macadamia nut green peel has an antibacterial effect for the first time, and the preparation method of the antibacterial agent comprises the following steps: cleaning the macadamia nut green peel, drying, crushing, sieving, collecting powder on the sieve, adding distilled water, carrying out a hydrothermal reaction, carrying out suction filtration after the reaction is finished, and collecting filtrate, so as to obtain the antibacterial agent. The antibacterial agent prepared from the macadamia nut green peel can effectively inhibit escherichia coli and staphylococcus aureus, and a new way is provided for resource utilization of the macadamia nut green peel.

The invention relates to the technical field of polyether-ether-ketone composite materials, in particular to a conjugated polythiophene-polyether-ether-ketone-based high-antibacterial coating and a preparation method thereof.The preparation method comprises the steps that hydroxyl modified boron nitride with photocatalytic capacity and aminated PEEK are subjected to melt blending to generate chemical bonding, and a BN-OH / PEEK-NH2 composite fine powder material is formed; then carrying out oxidative polymerization on the BN-OH / PEEK-NH2 composite fine powder and a thiophene monomer under the action of an oxidizing agent, carrying out in-situ synthesis to obtain a polythiophene / BN-OH / PEEK-NH2 conjugated composite material, and subsequently spraying the polythiophene / BN-OH / PEEK-NH2 conjugated composite material to the surface of a base material to prepare a composite coating with excellent antibacterial property, high thermal stability and low friction coefficient; the antibacterial rate of the coating on escherichia coli and staphylococcus aureus under illumination reaches 90% or above, and the coating is suitable for the medical antibacterial field.

The present invention discloses a broad-spectrum antibacterial peptide, and the amino acid sequence of the antibacterial peptide is GECEGNNRPVKKPQPRKKLPRFKKC-NH2. Based on melittin, the present invention designs antibacterial peptides by predicting based on a deep learning model, and obtains antibacterial peptides with broad-spectrum bacteriostasis, thermal stability and low hemolytic property, which have good inhibitory effects on skin pathogenic bacteria such as Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Propionibacterium acnes. Peptides predicted as AMP amino acid sequences by the deep learning model antibacterial peptide prediction tool amPEPpy are selected for screening, and by using a biosynthesis technique, they are expressed, extracted and purified in Escherichia coli, reducing the workload and screening period of screening, and increasing the probability of screening out broad-spectrum and thermally stable biosynthetic antibacterial peptides.

The invention relates to the technical field of nano-particles, and particularly provides a preparation method of luteolin-loaded mesoporous polydopamine nano-particles, which mainly comprises the following steps: adding mesoporous polydopamine nano-particles into a luteolin solution, stirring until luteolin is adsorbed onto mesoporous polydopamine, centrifuging, washing, and drying to obtain luteolin-loaded mesoporous polydopamine nano-particles. The luteolin-loaded mesoporous polydopamine nano-particles are obtained by adding the luteolin into the mesoporous polydopamine nano-particles; according to the luteolin-loaded mesoporous polydopamine nano-particles, the photo-thermal sterilization effect of the mesoporous polydopamine nano-particles is combined with the antibacterial effect of luteolin, so that the illumination sterilization effect at low temperature is achieved, and the luteolin-loaded mesoporous polydopamine nano-particles have an excellent antibacterial rate on staphylococcus aureus and escherichia coli.