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Nanosilver-containing antibacterial and antifungal granules and methods for preparing and using the same

a technology of nanosilver and antibacterial granules, which is applied in the directions of plant/algae/fungi/lichens ingredients, biocide, unknown materials, etc., can solve the problems of limited use of silver ions in solution, inability to ensure contact between microbial cells and silver ions, and inability to acquire resistance to metals

Inactive Publication Date: 2002-05-02
LEGEND WIN FINANCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0021] The nanosilver solution is prepared by the following step-wise procedure: (1) dissolving silver nitrate (AgNO.sub.3) crystal in a concentrated ammonia water solution; (2) adding glucose or ascorbic acid (as reducing agent) to the solution; and (3) adding an oxidizing agent to the solution. The preferred oxidizing agent is hydrogen peroxide (H.sub.2O.sub.2). Optionally, NaOH and ethanol can be added to the nanosilver solution to adjust the pH and improve the solubility of the nanosilver solution, respectively.

Problems solved by technology

Bacteria treated by these metals do not acquire resistance to the metals.
However, the contact between microbial cells and silver ions is not ensured as the silver ions quickly become eluted in the solution.
Silver ions in solution are difficult to handle and therefore of limited use.
Such solid supports using synthetic polymer materials have been not widely adapted for medical and healthcare purposes.
These materials also do not have sufficiently high antibacterial activity due to the lack of sufficient surface contact with the bacteria.
Additionally, the bactericidal activity of these materials rapidly diminishes as the silver ions become separated from the solid supports, thus, these materials do not show bactericidal activity over a prolonged period of time.
Lastly, the processes for making these materials are complicated and time-consuming.
However, the nitrates irritate the skin.

Method used

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  • Nanosilver-containing antibacterial and antifungal granules and methods for preparing and using the same
  • Nanosilver-containing antibacterial and antifungal granules and methods for preparing and using the same
  • Nanosilver-containing antibacterial and antifungal granules and methods for preparing and using the same

Examples

Experimental program
Comparison scheme
Effect test

example 2

Antibacterial Effect of the Solution Containing the NAGs

[0064] The antibacterial effect of the NAGs in the solution was tested as follows:

[0065] A. Preparation of the NAGs solution

[0066] Five (5) g of the NAGs were suspended in 500 ml of water and stayed in the solution for 24 hrs to obtain an NAGs-containing solution.

[0067] B. Preparation of bacteria

[0068] M-H liquid and solid culture media were used for activation and culture of the bacteria. Staphylococcus aureus stock M120, Escherichia coli stock E109, and Pseudomonas aeruginosa stock PS208 were used for the testing. These stocks were provided by the Microbiology Teaching and Research Section at Henan Medical University.

[0069] The frozen stocks of bacteria were seeded onto solid culture media. Single colonies were picked and inoculated respectively in 2 ml liquid culture medium and cultured at 37.degree. C. for eight (8) hours. Then, the liquid culture medium was diluted at 1:1000 and stored in refrigerator (around 0.degree. C.)...

example 3

Time Course of Antibacterial Effects of the NAGs

[0074] A time course study of the antibacterial effects of the NAGs in a solution was conducted as follows:

[0075] A. Preparation of bacterial stocks

[0076] M-H liquid and solid culture media were used for activation and culture of the bacteria. Staphylococcus aureus stock M120, Escherichia coli stock E109, and Pseudomonas aeruginosa stock PS208 were provided by the Microbiology Teaching and Research Section at Henan Medical University. The stocks were cultured in an incubator at 37.degree. C. for 24 hours.

[0077] The frozen stocks of bacteria were seeded to a culture plate. Single colonies were chosen and inoculated in 2 ml liquid culture medium, respectively, where they were cultured at 37.degree. C. for eight (8) hours. Then, the liquid culture medium was diluted at 1:1000 and stored in the refrigerator (around 0.degree. C.) until ready for use. The solution contained about 10.sup.5 CFU / ml bacterial stock.

[0078] B. The effect of the NA...

example 4

Antibacterial Effect of the NAGs on Toothbrush

[0085] The antibacterial effect of the NAGs was tested on toothbrush as follows:

[0086] A. Preparation of bacteria

[0087] M-H liquid and solid culture media were used for activation and culture of the bacteria. Staphylococcus aureus stock M120 was provided by the Microbiology Teaching and Research Section at Henan Medical University. The stock was cultured in an incubator at 37.degree. C. for 24 hours.

[0088] The frozen stock of bacteria was seeded to a culture plate. A single colony was chosen and inoculated in 2 ml liquid culture medium, respectively, where the bacteria were cultured at 37.degree. C. for eight (8) hours. Then, the liquid culture medium was diluted at 1:1000 and stored in the refrigerator (around 0.degree. C.) until ready for use. The solution contained about 10.sup.5 CFU / ml bacterial stock.

[0089] B. Treatment of toothbrush

[0090] The bactericidal activity of the NAGs on toothbrush was tested by two (2) methods:

[0091] (1). ...

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Abstract

The present invention relates to nanosilver-containing antibacterial and antifungal granules ("NAGs"). The NAGs have longlasting inhibitory effect on a broad-spectrum of bacteria and fungi, which include, but are not limited to, Escherichia coli, Methicillin resistant Staphylococcus aureus, Chlamydia trachomatis, Providencia stuartii, Vibrio vulnificus, Pneumobacillus, Nitrate-negative bacillus, Staphylococcus aureus, Candida albicans, Bacillus cloacae, Bacillus allantoides, Morgan's bacillus (Salmonella morgani), Pseudomonas maltophila, Pseudomonas aeruginosa, Neisseria gonorrhoeae, Bacillus subtilis, Bacillus foecalis alkaligenes, Streptococcus hemolyticus B, Citrobacter, and Salmonella paratyphi C. The NAGs contain ground stalk marrow of the plant Juncus effuses L. which has been dispersed with nanosilver particles. The nanosilver particles are about 1-100 mn in diameter. Each of the nanosilver particles contain a metallic silver core which is surrounded by silver oxide. The present invention also provides a process for making the NAGs. The NAGs can be used in a variety of healthcare and industrial products. Examples of the healthcare products include, but are not limited to, ointments or lotions to treat skin trauma, soaking solutions or cleansing solutions for dental or women hygiene, medications for treating gastrointestinal bacteria infections, sexual related diseases, and eye diseases. Examples of industrial products include, but are not limited to, food preservatives, water disinfectants, paper disinfectants, construction filling materials (to prevent mold formation).

Description

[0001] This application claims the priority of U.S. Provisional Application No. 60 / 230,925, filed on Sep. 13, 2000, which is herein incorporated by reference.[0002] The present invention relates to nanosilver particles-containing antibacterial and antifungal granules (NAGs). The nanosilver particles are attached to the surfaces and pores of stalk marrow of Juncus effuses L, which acts as an inert carrier for nanosilver. Each of the nanosilver particles contains a metallic silver core which is surrounded by silver oxide. The size of the nanosilver particle is between 1-100 nm in diameter. The present invention also relates to methods for preparing the NAGs and for using the NAGs. The NAGs can be used in a variety of healthcare, medicinal and industrial products.DESCRIPTION OF THE RELATED ART[0003] Metals including silver, copper, mercury, and zinc are known for anti-bacterial properties. Bacteria treated by these metals do not acquire resistance to the metals. Therefore, the bacteric...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K33/38
CPCA61K33/38Y02A50/30
Inventor YAN, JIXIONGCHENG, JIACHONG
Owner LEGEND WIN FINANCE
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