77 results about "Chlamydiae" patented technology

Methods are disclosed for sterilizing tissue to reduce the level of one or more active biological contaminants or pathogens therein, such as viruses, bacteria, (including inter- and intracellular bacteria, such as mycoplasmas, ureaplasmas, nanobacteria, chlamydia, rickettsias), yeasts, molds, fungi, spores, prions or similar agents responsible, alone or in combination, for TSEs and/or single or multicellular parasites. The methods involve sterilizing one or more tissues with irradiation.

The invention relates to a fluorescence PCR (polymerase chain reaction) detection method for diagnosing infection of Chlamydia trachomatis (CT), neisseria gonorrhoeae (NG) and ureaplasma urealyticum (UU), which belongs to the field of nucleic acid in vitro diagnosis. The method comprises a polymerase chain reaction (PCR) system based on fluorescence PCR technology, contains a forward primer and areverse primer aiming at the CT/the NG/the UU and a fluorescent probe, and can detects DNA of three pathogens such as the CT, the NG, the UU and the like simultaneously in a reaction tub under suitable PCR condition. The method can diagnosing the infection of the CT/the NG/the UU in a clinical sample simply, conveniently and rapidly, has high sensitivity and specificity, and has important clinical value to early control and prevention of relevant genitourinary tract infections, blocking of an infection sources, and infection reduction of related pathogen.

The invention discloses the Chlamydia PMPE and PMPI polypeptide, polypeptides derived therefrp, (PMP-derived polypeptides), nucleotide sequences encoding said polypeptides, antibodies that specifically bind the PMP polypeptides and PMP-derived polypeptides and T-cells specific for PMP polypeptides and PMP-derived polypeptides. Also disclosed are prophylactic and therapeutic compositions, including immunogenic compositions, e.g., vaccines, comprising PMP polypeptides or PMP-derived polypeptides or antibodies thereto. The invention additionally discloses methods of inducing in animals an immune response to Chlamydia cells, Chlamydia elementary bodies, and/or cells expressing Chlamydial proteins, e.g., cell infected with Chlamydia.

The present invention relates to a staining kit and its preparation method, staining method and its application in several detection items for detecting canceration cell, red blood cell, campylobacteriosis, vaginal cleaning degree, estrogen level, garlandosus, trichomoniasis, mycosis, diplococcus gonorrhoeae and chlamydi trachomatis on a cervical smear. Said invented kit is formed from fixatino fluid, staining solution and differentiatino solution. Its staining method includes the steps of fixation, staining, differentiation and water-washing. Its film-making speed is quick, only has need oftwo main, and its detection accuracy rate is high, can be up to 98%.

The present invention relates to secreted Chlamydia polypeptides, which may be expressed by a Gram-negative bacterial strain and secreted by the type III secretion pathway of said bacterial strain. The present invention also relates to polynucleotides coding for these polypeptides, as well as to the therapeutic and vaccination uses of these secreted Chlamydia polypeptides.

The invention discloses a chlamydia pneumoniae IgM (immunoglobulin M) colloidal golden method kit which comprises recombinant chlamydia pneumoniae antigens enveloped on a nitrocellulose membrane detection line, goat anti-rat IgG (immunoglobulin G) antibodies enveloped on a quality control line and rat anti-human IgM monoclonal antibodies which have colloidal gold labels and are enveloped on a gold label pad, the concentration of the chlamydia pneumoniae antigens ranges from 1mg/ml to 2mg/ml and is measured by SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis), the concentration of the goat anti-rat IgG (immunoglobulin G) antibodies ranges from 1mg/ml to 3mg/ml, and the concentration of the rat anti-human IgM monoclonal antibodies ranges from 5g/mL to 30g/mL and is measured by the SDS-PAGE. The chlamydia pneumoniae IgM colloidal golden method kit has the advantages that the kid is speedy, simple, convenient and accurate and is high in sensitivity, and a judgment result can be read after integral operation time of dozens of minutes; and a colloidal gold quick detecting paper strip is made of the multi-epitope recombinant antigens and is simple and convenient to operate, low in cost, good in specificity and high in sensitivity, can be used for single-component detection and is popularized easily, and detection and control effects for chlamydia pneumoniae IgM are obvious.

Compositions and methods for detecting Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) are provided. The present invention also provides methods and compositions for screening for infection/inflammation based on genomic copy number. Described herein is a method that entails assaying a sample obtained from the urogenital tract of the mammal for an indicator of genomic copy number, wherein a genomic copy number level that is higher than a control genomic copy number level is indicative of the presence of infection or inflammation of the urogenital tract. Also described in a kit of the invention that includes a primer and/or probe for detecting or sequencing an indicator of genomic copy number, wherein the indicator of genomic copy number comprises a nucleic acid sequence that is expected to be present in the genome of the mammal in one or two copies; and a primer and/or probe for detecting or sequencing a nucleic acid sequence that is indicative of a pathogen that infects the urogenital tract or a miRNA correlated with inflammation.

The invention discloses a bivalent inactivated vaccine for goat mycoplasma pneumonia and chlamydia psittaci and a preparation method thereof. The active ingredients of the bivalent inactivated vaccinecomprise inactivated Mycoplasma capricolum subsp. Capripneumoniae, inactivated mycoplasma ovipneumoniae and inactivated MOMP protein of chlamydia psittaci. The amino acid sequence of the MOMP proteinof the chlamydia psittaci is shown as a sequence 2 in a sequence table. The mycoplasma ovipneumoniae is mycoplasma ovipneumoniae SH-01 CGMCC No.16503. The Mycoplasma capricolum subsp. Capripneumoniaeis Mycoplasma capricolum subsp. Capripneumoniae GT-01 CGMCC No.16504. Experiments prove that the bivalent inactivated vaccine has good safety in immunizing experimental animals and target animals, and can effectively prevent goat mycoplasma pneumonia and chlamydia psittaci from infecting the experimental animals and the target animals. The vaccine has important significance for preventing and controlling prevalence and propagation of mycoplasma pneumonia and chlamydia psittaci of goats. The vaccine has important application value.

The invention relates to the technical field of medical biology, and especially relates to a pathogen nucleic acid-drug resistant gene detection kit and an application technology thereof. The kit designed by the invention includes the following two kinds of compositions: a PCR reaction buffer solution, an enzyme mixed solution, and a chlamydia trachomatis/mycoplasma urealytium/mycoplasma hominis/drug resistant gene multiple reaction solution; or a PCR reaction buffer solution, an enzyme mixed solution, a chlamydia trachomatis/mycoplasma urealytium/mycoplasma hominis multiple reaction solution, and a drug resistant gene reaction liquid. The kit adopts a multi-fluorescence quantitative PCR technology, can simultaneously detect multiple target genes in a same PCR reaction tube, and provides a powerful technical support for simultaneous detection of pathogens and drug resistant genes. A method has the characteristics of less required amount on samples, low cost, simple and convenient operation, high sensitivity and good specificity, and has extremely great social and economic significances.

The invention relates to test paper for detecting chlamydia trachomatis infection, and the test paper comprises a baseplate, a sample pad, a conjugate pad, a nitrocellulose membrane and an absorption pad, wherein a detection line and a quality control line are arranged on the nitrocellulose membrane, the detection line on the nitrocellulose membrane is coated by mouse anti-human monoclonal protein antibody IgG (antibody II) of major outer membrane protein antigen of chlamydia trachomatis in the concentration of greater than or equal to 1mg/mL, and the quality control line is coated by goat anti-mouse IgG polyclonal antibody in the concentration of greater than or equal to 1.5mg/mL; the conjugate pad is coated by mouse anti-human monoclonal protein antibody IgG (antibody I) of the MOMP (major outer membrane protein) antigen of the chlamydia trachomatis, which is labeled by nano-gold and is in the concentration of not less than 6 mu g/mL; the sensitivity of detecting the MOMP of the chlamydia trachomatis of the test paper is 0.1ng/ml, the detection value range is 0-200ng/ml and the specificity is 98.5%; and the detection can be completed within 5 minutes.

A degerming dustproof a multifunctional electronic mask is characterized in that an outer layer body 1, an inner layer body 2, an isolation layer 3, an air hole 4, an air hole 5, an air hole 6, a negative electrode 7, an adsorption body 8, a positive electrode 9, an adsorption body 10, a supporting body 11, ear bands 12, a power supply 13 and a zipper 14 are connected into a whole to form the degerming and dustproof multifunctional electronic mask. The degerming dustproof multifunctional electronic mask which is wide in degerming range and various in dustproof variety. Degerming comprises non-cell microorganisms (various viruses); prokaryotic cell type microorganisms (various bacteria, mycoplasma, chlamydia, rickettsia, spirochete and actinomycetes); eukaryotic cell type microorganisms (fungi) and various bacterial spores. Dust prevention includes a variety of oily, non-oily contaminants in air from particles greater than 5 microns to particles less than 1 micron. The degerming dustproof multifunctional electronic mask is suitable for medical treatment and public health, production labor, household protection, fitness and physical exercise. The degerming dustproof multifunctional electronic mask is wide in manufacturing material and low in production cost, the mask body is made of elastic non-breathable ultrathin insulating material fibers, fabrics and films, the electrodes aremade of metal sheets, metal coating films or conductive carbon fibers, and the adsorption body is made of paper fibers (used in dry seasons in winter) or chemical fibers (used in rainy seasons in summer). The power supply is a general mobile phone lithium battery and a high-voltage electron generator.

The invention discloses a traditional Chinese medicine composition gelata for treating gynecological inflammations. The traditional Chinese medicine composition gelata for treating gynecological inflammations comprises a gel matrix and medicinal raw materials of 1750 to 2250 parts by weight of cortex ailanthi, 1250 to 1750 parts by weight of sophora flavescens, 1250 to 1750 parts by weight of frankincense, 40 to 60 parts by weight of borneol and 1250 to 1750 parts of tree peony bark. The traditional Chinese medicine composition gelata for treating gynecological inflammations overcomes defects of chemical drugs, can produce multiple treatment effects of clearing heat, removing dampness, reducing leucorrhea, relieving itching, removing slough and promoting tissue regeneration, wherein the multiple treatment effects are superior to treatment effects produced by chemical drugs, and can be utilized for treating gynecological inflammations caused by pathogens such as bacteria, fungi, protozoa, chlamydiae and mycoplasmas, wherein the gynecological inflammations comprise internal damp-heat downward flow-caused vaginal discharge diseases such as bacterial vaginosis, vulvovaginal candidasis and the like.

The present invention provides variant LcrE sequences and/or combinations of variant LcrE sequences across the Chlamydia trachomatis serovars. Such changes in LcrE genotypes across the Chlamydia trachomatis serovars are likely to correspond with changes in LcrE phenotypes, in particular, with changes in immunogenicity. The present invention also provides peptides surrounding or associated with the amino acid substitutions, in particular, peptides surrounding or associated with high frequency mutated amino acid positions or hypervariable regions, which are likely to be B and/or T cell epitopic regions capable of eliciting Chlamydia specific cell mediated immune responses. The variant LcrE sequences and/or combinations of the variant LcrE sequences and/or epitope regions associated with the variant LcrE sequences are useful as immunogens and/or in the preparation of immunogenic compositions for preventing and/or treating and/or, diagnosing Chlamydia infections.

The invention provides a silver ion antibacterial gel. The silver ion antibacterial gel is prepared from, by mass, 0.5-0.6 part of soluble silver salt, 7-8 parts of carbomer, 50-150 parts of propylene glycol, 50-150 parts of glycerin, 15-20 parts of menthol, 15-20 parts of radix sophorae flavescentis, 3-15 parts of peach kernels and 10-20 parts of herba taraxaci. The silver ion antibacterial gel is reasonable in formula and easy to prepare and has a strong killing effect on pathogenic microorganisms such as bacteria, fungi, candida, viruses, mycoplasmas and chlamydiae. The silver ion antibacterial gel is suitable for gynecological inflammations and vaginal infections caused by the various pathogenic microorganisms.

The invention provides a kit for rapidly detecting urogenital tract pathogens. The kit is characterized by comprising PCR reaction liquid, Taq/UDG enzyme mixture liquid, positive control and negativecontrol; the PCR reaction liquid comprises 10*PCR buffer, a primer and a probe of chlamydia trachomatis CT, a primer and a probe of gonococcus NG, a primer and a probe of ureaplasma urealyticum UU, aprimer and a probe of human beta-globin HBB, 25 mM MgCl<2>, dNTPs (dATP, dGTP, dCTP, dTTP, dUTP mixtures), and sterile distilled water. The kit in the invention has the advantages that: sample nucleicacid does not need to be extracted; detection is carried out after direct treatment; operation is simple, convenient and rapid; a single tube can detect multiple pathogens at the same time; the kit is economic and high-efficiency; high-flux sample detection can be carried out; the detection efficiency is increased; and the kit is good in specificity, high in pollution and interference resistance,good in result repeatability, and relatively precise.

The invention belongs to the field of medical detection equipment, and provides a combined device for synchronously detecting IgM antibodies of influenza A and B viruses and chlamydia pneumoniae and mycoplasma pneumoniae. The device comprises a double-channel clamping shell, a test strip FluA & B and a test strip CP & MP which are arranged in the double-channel clamping shell in parallel; influenza virus A detection lines arranged on a nitrocellulose membrane of the test strip FluA & B at intervals are coated with high-specificity influenza virus A antigens, influenza virus B detection linesare coated with high-specificity influenza virus B antigens, and first quality control lines are coated with quality control line coating receptors; chlamydia pneumoniae detection lines (CP) arranged on a nitrocellulose membrane of the test strip CP & MP at intervals are coated with high-specificity chlamydia pneumoniae antigens, mycoplasma pneumoniae detection lines (MP) are coated with high-specificity mycoplasma pneumoniae antigens, and second quality control lines are coated with quality control line coating receptors.

The invention discloses a bactericidal instrument panel or camera window and a manufacturing method for the wearable instrument panel or camera window. The bactericidal instrument panel or camera window comprises a substrate, wherein the outer surface of the substrate is sequentially provided with a first film layer, a second film layer and a third film layer from inside to outside; the first film layer is a nano silver layer with a thickness of 5-20nm; and the second film layer is a hard-hardness layer with a thickness of 10-50nm. According to the technical scheme, the first film layer is the nano sliver layer which has strong inhibiting and killing effects on ten pathogenic microorganisms such as escherichia coli, gonococci, chlamydia trachomatis and the like without generating drug tolerance; the thickness of the first film layer is 5-20nm, so that enough bactericidal ability of the instrument panel or camera window is guaranteed; the second film layer is the high-hardness layer, so that the instrument panel or camera window has the wearable effect; and the thickness of the second film layer is 10-50nm, so that enough wear resistance of the instrument panel or camera window is guaranteed. The bactericidal instrument panel or camera window is reasonable design and high in practicability.

The invention relates to application of a Chinese medicinal composition in preparing a gel for treating gynecological inflammation, wherein, the Chinese medicinal composition is prepared by the following bulk medicines by weight: 2000 parts of cortex ailanthi, 1500 parts of sophora flavescens, 1500 parts of frankincense, 50 parts of borneol, and 1500 parts of cortex moutan. The gel prepared by the Chinese medicinal composition in the invention can overcome many defects of chemical medicines, exert the incomparable extensive curative effects (such as heat clearing and dehumidifying, leucorrhea stopping and itching relieving, necrotic tissue removing and granulation promoting) of chemical medicines, and can be widely applied in treating gynecological inflammation (such as damp-heat pouring downward leukorrhagia including bacterial vaginosis, vulvovaginal candidiasis, etc.) caused by bacteria, fungi, protozoa, chlamydiae, mycoplasmas and other pathogens.

In alternative embodiments, provided are pharmaceutical compositions and methods for treatment, amelioration and prevention of infection-associated blood vessel diseases, and also for the treatment, amelioration and prevention of non-vessel diseases affected by infective agents which can be treated by these compositions. In alternative embodiments, one common pathogen targeted by compositions and methods as provided herein is Chlamydia and Chlamydophila species, including pneumoniae, trachomatis and psittaci species which infect humans, including Chlamydophila penumoniae which also infects humans. In alternative embodiments, pathogens targeted and infections (diseases) treated ameliorated, or prevented by compositions and methods as provided herein include Mycoplasma, Listeria, Leptospirosis, Q fever or Coxiella burnetii infection, Lyme disease or Lyme borreliosis or any Borrelia infection, and Bartonella or of the family Bartonellaceae, including cat scratch disease.

The invention discloses a new disinfection material for a wet wipe and a preparation method of the new disinfection material. The wet disinfection wipe is made of a fiber tower by adsorbing the disinfection material. The disinfection material is prepared from the raw materials in parts by weight as follows: 3-8 parts of tea polyphenol, 20-35 parts of herba lobeliae chinensis extracts, 10-20 parts of inula japonica extracts, 10-20 parts of incense tube herb extracts, 2-6 parts of turpentine, 3-5 parts of limonene, 10-20 parts of pseudechinolaena polystachya and 60-100 parts of a 70% ethanol aqueous solution. The new disinfection material for the wet wipe adopts a simple preparation method, is convenient to use, can kill a variety of pathogenic bacteria such as staphylococcus aureus, viruses, spirochete, rickettsia, chlamydiae, mycoplasmas, fungi, actinomycetes and the like and has high killing efficiency.

The invention provides a chlamydia pneumoniae quantum dot immunochromatographic assay detection card and a preparing method and application thereof. The detection card comprises a bottom board, a sample pad, a water absorbing pad, a combining pad and a detection layer. Chlamydia pneumoniae resisting nanometer probes labeled with quantum dots envelop combining pad. The detection layer is formed by a solid-phase nitrocellulose membrane with a detection line and a quality control line. Anti-mouse chlamydia pneumoniae 98KD membrane protein polyclonal antibodies envelop the detection line. Anti-rabbit IgG is encapsulated in the quality control line. The detection layer is bonded to the bottom board. The combining pad and the water absorbing pad are arranged on the upper portions of the two ends of the detection layer respectively, partially overlapped with the detection layer and then bonded to the detection layer and the bottom board. The sample pad is arranged on the combining pad, partially overlapped with the combining pad and then bonded to the combining pad and the bottom board. The chlamydia pneumoniae quantum dot immunochromatographic assay detection card has the advantages of being easy and convenient to operate, fast in detection, capable of achieving quantification, high in sensitivity and the like.

The invention belongs to the field of immunology, and more specifically discloses a hybridoma cell strain, and an anti-chlamydia abortus monoclonal antibody secreted by the hybridoma cell strain. According to a preparation method, purified prokaryotically expressed chlamydia abortus major outer membrane protein (MOMP) is used for immunization of mouse as an antigen, indirect ELISA is adopted for screening of positive clones, and the hybridoma cell CA1-MM 01 capable of secreting the monoclonal antibodies aiming at chlamydia abortus major outer membrane protein is obtained. The identification ofthe monoclonal antibody in the aspects of the stability of the hybridoma cell after continuous continuous passage culture and cryopreservation, and the subtype and titer of the monoclonal antibody iscarried out, it is shown by results that the capacity of the hybridoma cell in secretion of antibodies is excellent and stable, the monoclonal antibody titer is high. It is shown that the obtained monoclonal antibody possesses extremely high specificity and combination capacity with chlamydia abortus.