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8 results about "Chlamydiae" patented technology

The Chlamydiae are bacterial phylum and class whose members are a group of obligate intracellular bacteria, whose members are remarkably diverse, ranging from pathogens of humans and animals to symbionts of ubiquitous protozoa. They are ovoid in shape and stain Gram-negative. Historically it was believed that all Chlamydiae species had a peptidoglycan-free cell wall, but recent work demonstrates a detectable presence of peptidoglycan, as well as other important proteins. Many species belonging to this order are susceptible to antimicrobial agents. All known Chlamydiae only grow by infecting eukaryotic host cells. They are as small as or smaller than many viruses. They are dependent on replication inside the host cells, thus some species are termed obligate intracellular pathogens and others are symbionts of ubiquitous protozoa. Most intracellular Chlamydiae are located in an inclusion body or vacuole. Outside cells, they survive only as an extracellular infectious form. Chlamydiae can grow only where their host cells grow, and develop according to a characteristic biphasic developmental cycle. Therefore, Chlamydiae cannot be propagated in bacterial culture media in the clinical laboratory. Chlamydiae are most successfully isolated while still inside their host cells. Chlamydiae is the most common bacterial STD in the United States and 2.86 million chlamydiae infections are reported annually.

Primer probe group and kit for joint detection of mycoplasma genitalium, ureaplasma urealyticum and chlamydia trachomatis

The invention discloses a primer probe group and a kit for joint detection of mycoplasma genitalium, ureaplasma urealyticum and chlamydia trachomatis, and belongs to the technical field of molecular biological detection. The primer probe group comprises a primer probe group A for directly detecting a mycoplasma genitalium mgpB gene, a primer probe group B for directly detecting a ureaplasma urealyticum ureB gene and a primer probe group C for directly detecting a chlamydia trachomatis ompA gene; the kit comprises a PCR (Polymerase Chain Reaction) reaction solution and the primer probe group. Nucleic acid extraction is not needed, the detection cost is greatly reduced, the experiment time is shortened, meanwhile, the specificity and accuracy are still guaranteed, meanwhile, the sensitivity is improved, and the method has wide application prospects in the fields of non-gonococcal genital tract infection main pathogen detection, infertility and prenatal and postnatal care screening and the like. And the kit has important significance on clinical diagnosis, early treatment and prevention of diseases, prevalence and the like.
Owner:ZHEJIANG ANJI JIJIAN MEDICAL TECH CO LTD

Kit for rapidly and jointly detecting multiple pathogen nucleic acids related to dyspnea pregnancy outcome and application of kit

PendingCN121272078AMicrobiological testing/measurementMicroorganism based processesNucleic acid amplification techniquePregnancy
The invention belongs to the technical field of in-vitro diagnostic reagents for rapid detection of human pathogenic microorganisms, and particularly relates to a kit for rapid combined detection of multiple pathogen nucleic acids related to a dyspnea pregnancy outcome and application of the kit. The pathogen is selected from one or more of escherichia coli, candida albicans, candida glabrata, group B streptococcus, streptococcus gonorrhoeae, chlamydia trachomatis or ureaplasma urealyticum, and the kit comprises a pathogen and lactobacillus rapid detection micro-fluidic chip. A primer group for detecting the pathogen and the lactic acid bacillus nucleic acid and an array consisting of an amplification control and a blank control are fixed on the micro-fluidic chip. According to the present invention, the microfluidic chip rapid detection technology and the nucleic acid amplification technology are adopted to achieve the purposes of rapid detection and multiple detection so as to rapidly and conveniently determine the poor prognosis risk of the pregnant woman at present, timely take the specific measures, and avoid the occurrence of the poor prognosis.
Owner:PEKING UNIVERSITY THIRD HOSPITAL (THE THIRD CLINICAL MEDICAL SCHOOL OF PEKING UNIVERSITY) +1

Microflora prediction model for detecting premature delivery risk and kit and application thereof

PendingCN121975957Agood forecastMicrobiological testing/measurementMicroorganism based processesHemolytic streptococcusEnterobacter
The invention relates to a flora prediction model for detecting premature delivery risk and a kit and application thereof. Through early-stage mNGS data collection and analysis, premature related strains are obtained, and through a large number of experimental screening and optimization, a set of optimal primer probe group is finally determined. The invention relates to a premature delivery risk calculation method, which comprises the following steps of: selecting 12 floras, namely lactobacillus crispatus, lactobacillus gasseri, lactobacillus inertus, lactobacillus jensenii, escherichia coli, enterococcus faecalis, enterobacter aerogenes, group B hemolytic streptococcus, ureaplasma parvum, chlamydia trachomatis, diplococcus gonorrhoeae and gardnerella vaginalis, and establishing a fluorescent quantitative PCR (Polymerase Chain Reaction) method and a premature delivery risk calculation model aiming at the detection of the 12 floras. Compared with an existing detection method, the method is simple, convenient, rapid, high in sensitivity and high in specificity, the false positive and false negative risk is reduced, and the purpose of batch detection is achieved. Clinically, the change of microbial flora in the reproductive system of a pregnant woman can be rapidly detected, corresponding treatment measures are taken, the risk of PTB occurrence is reduced, and the kit has a good application prospect.
Owner:SHANGHAI FIRST MATERNITY & INFANT HOSPITAL

Primer composition for detecting chlamydia trachomatis, gonococcus and ureaplasma urealyticum and use thereof

The application provides a primer composition for detecting chlamydia trachomatis, gonococcus and ureaplasma urealyticum and application thereof, and relates to the technical field of nucleic acid determination or detection methods.The primer composition provided by the application comprises a first primer and a second primer for detecting chlamydia trachomatis, a third primer and a fourth primer for detecting gonococcus and a fifth primer and a sixth primer for detecting ureaplasma urealyticum.The primer composition provided by the application can realize the detection of the above-mentioned three pathogens, and has the characteristics of good specificity, high sensitivity and good repeatability.Based on the primer composition, the application further combines an immunochromatography test strip detection technology, so that rapid and efficient detection of chlamydia trachomatis, gonococcus and ureaplasma urealyticum can be realized, the operation is simple and relatively portable, and the primer composition can be used for home self-detection.
Owner:PEKING UNIVERSITY FIRST HOSPITAL (PEKING UNIVERSITY FIRST CLINICAL MEDICAL COLLEGE)

Primer composition, kit for efficiently detecting common pathogenic bacteria of human and application thereof

The application provides a primer composition, a kit and application thereof for efficiently detecting common pathogenic bacteria of human, relates to the technical field of microorganism gene detection, and aims at solving the defects of low detection efficiency, poor specificity, low sensitivity of traditional PCR technology, and non-specific amplification of traditional LAMP detection technology. The application designs primers according to known sequences of six common pathogenic bacteria of human, i.e., Candida albicans, Chlamydia trachomatis, Escherichia coli, group B streptococcus, Mycoplasma hominis and Streptococcus pneumoniae, and provides a kit with a micro-fluidic chip as a carrier and application thereof, which are used for detecting common pathogenic bacteria of human, have high specificity and high sensitivity, reduce detection cost and detection time, and can realize on-site rapid detection of common pathogenic bacteria of human.
Owner:NINGBO AI GENE TECH CO LTD

Chlamydia trachomatis sample collection device

The invention discloses a chlamydia trachomatis sample collection device, and relates to the technical field of sample collection, the chlamydia trachomatis sample collection device comprises a shell and a built-in groove, the top of the shell is provided with a top plate, the top plate is internally provided with a driving assembly for providing driving force, and the driving assembly comprises an auxiliary plate, a toothed plate and a threaded rod. According to the chlamydia trachomatis sample collecting device, a threaded rod of the driving assembly drives a toothed plate to move, the auxiliary assembly and the flaring assembly can be synchronously linked, the toothed plate is meshed to drive a driving gear to rotate, on one hand, a threaded column is driven to push a swab to move towards a urethral orifice, on the other hand, two sets of racks are driven to move reversely, and a flaring piece completes urethral orifice flaring; the flaring and collecting actions are synchronously carried out, step-by-step operation is not needed, the overall time consumption of sample collection is shortened, the problem of collection failure caused by body position change or instrument displacement in the step-by-step operation process is solved, and the efficiency and success rate of chlamydia trachomatis sample collection are effectively improved.
Owner:SHANGHAI CHEMTRON BIOTECH

Oligonucleotides for detecting Chlamydia pneumoniae

To provide oligonucleotides for the detection of Chlamydia pneumoniae. [Solution] A probe for detecting the tyrp gene of Chlamydia pneumoniae is disclosed. The probe preferably has the following base sequence (A) or (B): (A) an oligonucleotide containing a base sequence S1 of at least 10 consecutive bases in a specific base sequence or a base sequence complementary thereto, or a base sequence S2 in which 1 to 3 bases are substituted, deleted, inserted, or added in base sequence S1. (B) only the 5' end or 3' end of the oligonucleotide of (A) is labeled.
Owner:TOYOBO CO LTD +1

Nucleic acid mixed detection method for genital tract chlamydia trachomatis infection

The invention provides a nucleic acid mixed detection method for genital tract chlamydia trachomatis infection, and belongs to the technical field of pathogen detection. The method comprises the following steps: mixing a to-be-detected sample and dividing the sample into six mixed sample pools, and detecting the mixed sample to obtain a positive mixed sample and / or a negative mixed sample; and performing single sample detection on a single sample corresponding to the positive mixed sample. According to the method, 6 mixing (5: 1) is taken as the optimal mixing detection proportion, the sensitivity of mixing detection is 96.70%, and the specificity is 100%; after 9 samples are detected again, the sensitivity is improved to 98.40%, the total coincidence rate is 98.40% (95% CI: 96.5%-100%), and the accuracy requirement of chlamydia trachomatis qualitative detection registration is basically met. The mixed detection technology can increase the number of screened people and the number of positive examples by three times, can save 1 / 3 of reagent cost, and has certain hygienic economic significance.
Owner:SHENZHEN NANSHAN DISTRICT CHRONIC DISEASE CONTROL CENT (SHENZHEN NANSHAN DISTRICT MENTAL HEALTH CENT)