32 results about "Sensitive cell" patented technology

Each smallest repeating unit (SRU) in a colour filter array has a first sub-unit (FSU) and a second sub-unit (SSU). For a first sub-image, first image data is read out from photo-sensitive cells that correspond to FSUs of SRUs lying in a row (R1-R3) or column, and by skipping reading out from photo-sensitive cells that correspond to SSUs of a first predefined percent (FPP) of the SRUs, while reading out the first image data from photo-sensitive cells that correspond to SSUs of a remainder of the SRUs. For a second sub-image, second image data is read out from photo-sensitive cells that correspond to FSUs of the FPP, whilst skipping reading out from photo-sensitive cells that correspond to FSUs of the remainder, and by skipping reading out from photo-sensitive cells that correspond to the SSUs of the SRUs. Sub-pixel shift is performed between the sub-images. Image(s) are then generated from the sub-images.

The invention relates to a novel ferrocenyl hydrophobic label degradation agent targeting GPX4 as well as a preparation method and application of the novel ferrocenyl hydrophobic label degradation agent. The GPX4 protein degradation agent has a chemical structure as shown in a formula (I). Pharmacological experiments show that the derivative or salt disclosed by the invention can be used for remarkably degrading GPX4 protein in ferroptosis sensitive cells (such as HT1080, OS-RC-2, 4T1 and 786-O), and has very strong anti-proliferative activity on the four cell strains. The invention also provides a preparation method of the derivative and the pharmaceutically acceptable salt thereof, and an application of the derivative and the pharmaceutically acceptable salt thereof as a GPX4 degradation agent. Formula (I).

The invention belongs to the technical field of biology, and particularly relates to preparation of DMSO-free cryopreservation liquid and application of the DMSO-free cryopreservation liquid in cell cryopreservation. The invention provides a DMSO (dimethyl sulfoxide)-free serum-free cell cryopreservation solution. Each 100 mL of the cell cryopreservation solution contains 1-100 mg of thermo-sensitive nanogel, 0.1-10 mg of trehalose, 10-100 [mu] L of glycerol and a basic culture medium. The nanogel (preferably pNIPAM) forms a protective layer at low temperature to inhibit ice crystal damage, and is rapidly dissociated during rewarming; and trehalose-glycerol synergistically stabilizes cell membranes. The cryopreservation liquid does not need programmed cooling, does not need centrifugation after resuscitation, is suitable for sensitive cells such as CAR-T cells and stem cells, has the resuscitation survival rate of more than 90%, and completely avoids the toxicity of DMSO and the risk of serum pollution.

The application discloses a PIP-seq technology-based adjustable osmotic pressure single-cell sequencing kit and a detection method, and through a wrapping buffer with adjustable osmotic pressure and a non-toxic digestion system, high-throughput sequencing of marine high-osmotic living cells and freshwater low-osmotic living cells without chemical fixation is realized for the first time, and the retention rate of sensitive cell groups (such as stinging cells and freshwater ion-regulating cells) is doubled. By only changing the buffer formula, marine and freshwater samples can be seamlessly switched without changing the core reaction process, and the kit has strong versatility, and successfully solves the osmotic pressure barrier problem that has long plagued the field of aquatic biology.

The application belongs to the technical field of cell culture, and particularly relates to a temperature-sensitive cell three-dimensional culture support, which comprises temperature-sensitive hydroxypropyl chitin, the molecular weight of the temperature-sensitive hydroxypropyl chitin is 15,000-300,000 Da, and the degree of deacetylation (DD) is 35%-65%. The support material is temperature-sensitive hydroxypropyl chitin prepared by derivatization of chitosan of a natural source through a specific process, and can be used for three-dimensional culture of cells. The temperature-sensitive hydroxypropyl chitin is used for three-dimensional culture of cells, and can significantly reduce the culture cost, improve the convenience and the viability of the cultured cells.

This provides a continuous culture process for culturing populations of shear-sensitive cells. [Solution] A continuous culture process for culturing a population of cells consisting of shear-sensitive cells, comprising controlling the gas outflow rate of a bioreactor system so that the gas outflow rate does not exceed 20 m / s, wherein the bioreactor system includes at least 25 L of culture medium, and the population of cells is 20 × 10 6 From cells / mL to 15 × 10 7 The process is characterized by achieving a steady-state viable cell concentration within the range of cells / mL, and the gas efflux rate being controlled at least until the bioreactor system reaches steady-state conditions.

Disclosed is an imaging system including an image sensor with a plurality of photo-sensitive cells arranged on a photo-sensitive surface of the image sensor; a light valve device with an array of light valves, wherein the array of light valves is arranged on an optical path of the photo-sensitive surface of the image sensor; and at least one processor configured to: control an exposure of photo-sensitive cells of at least a first region in the photo-sensitive surface by utilising respective light valves of said array; read out image data from the photo-sensitive cells of the image sensor; and process the image data to generate an image.

The invention belongs to the technical field of biology, and discloses an influenza B virus sensitizing cell strain as well as a construction method and application thereof. The influenza B virus sensitizing cell strain STX-BB2 can be used for stably expressing influenza virus receptor ST6GAL1 protein in a human source and controllably inducing and expressing influenza B virus PB2 protein by using doxycycline; the preservation number of the cell strain STX-BB2 is GDMCC (China General Microbiological Culture Collection Center) NO: 67100; the cell strain can enhance the expression ability of a host receptor and the virus replication key gene level at the same time; the infection efficiency and the replication titer of the influenza B virus in MDCK cells can be improved; the method can be widely applied to influenza B virus separation, mechanism research, vaccine seed virus amplification and drug screening model construction.

According to one embodiment, a nucleic acid construct is used for detecting an estrogen-sensitive cell. The nucleic acid construct includes an enhancer sequence, a promoter sequence and a reporter gene. The enhancer sequence includes at least an ERE sequence and at least a XRE sequence. The promoter sequence is ligated to downstream of the enhancer sequence. The reporter gene is ligated to downstream of the promoter sequence.

The present disclosure provides, among other things, continuous culture methods for producing a cell product, e.g., a recombinant protein, e.g., a glycoprotein, e.g., an antibody agent or a fusion protein. In some instances, methods herein allow large-scale production of a recombinant protein using continuous culture methods. The present disclosure identifies and addresses a problem with current continuous cell culture techniques in that at large-scale culture of certain cells have insufficient viable cell concentrations and impaired cell viability. The present disclosure provides, in part, methods and systems for large-scale continuous culture of shear-sensitive cells.

This invention relates to the field of biomedicine, specifically to a synergistic and targeted epigenetic pharmaceutical composition for the treatment of lung cancer. In studies of drug-resistant lung cancer cells, it was found that overexpression of the NSUN7 gene in drug-resistant lung cancer cells can inhibit MZF1. L The expression of MZF1 also promotes S When the expression of NSUN7 gene was suppressed, no promotion of MZF1 was found. L The expression of MZF1 can be suppressed at the same time. S The expression of NSUN7 gene was inhibited, meaning no opposite result was obtained. However, when NSUN7 gene expression was inhibited and EGFR-TKI inhibitors were administered to drug-resistant lung cancer cells, MZF1 cells in drug-resistant lung cancer cells showed a positive result. L Upregulation, MZF1 S Decreased expression of NSUN7 leads to the transformation of drug-resistant lung cancer cells into drug-sensitive cells. Therefore, interfering with NSUN7 in combination with epigenetic drugs to regulate the alternative splicing of methylation-sensitive transcription factors can increase the specificity of regulatory targets and synergistically enhance the in vitro and in vivo antitumor effects.

The present invention relates to a cell for measuring botulinum toxin activity that overexpresses SEPTIN2 or Thioredoxin (TXN), and a method for measuring botulinum toxin activity, comprising the steps of treating the cell for measuring botulinum toxin activity with botulinum toxin and culturing it, lysing the cultured cells to collect a cell lysate, and measuring the amount of SNAP-25 cleavage products in the cell lysate.

The invention provides a tick-borne encephalitis virus monoclonal antibody and application thereof, and relates to the field of biotechnology and virology. A mouse monoclonal antibody mAb-16 with specific neutralizing activity is screened, the monoclonal antibody mAb-16 mainly aims at envelope protein E of tick-borne encephalitis virus, the monoclonal antibody mAb-16 can specifically recognize and neutralize domain III in E protein EDIII of tick-borne encephalitis virus, and a biological membrane layer interference experiment shows that the monoclonal antibody mAb-16 has relatively strong interaction with the domain III of the E protein. The median inhibition concentration of the monoclonal antibody mAb-16 on primary viruses cultured by sensitive cells is 92.80 ng / mL; in a lethal mouse infection model, a high-dose group has complete protection activity and can be used for preparing tick-borne encephalitis virus detection reagents or related drugs for neutralizing and inhibiting tick-borne encephalitis viruses, so that prevention and treatment of tick-borne encephalitis viruses are facilitated.

The present application relates to the technical field of cell culture, and discloses a preparation method of a temperature-sensitive cell culture device, which relates to a mold and a culture sheet. One side of the culture sheet is coated with a temperature-sensitive liquid layer. The mold is provided with a mold cavity, and the mold cavity is provided with a groove for accommodating the culture sheet. The preparation method comprises the following steps: placing the culture sheet in the groove, with the side coated with the temperature-sensitive liquid layer facing the bottom of the groove; injecting a molten material into the mold cavity, and the molten material forms the cell culture device after cooling; and when the cell culture device is formed, the culture sheet is located at the bottom of the concave cavity of the cell culture device and is combined with the cell culture device to form an integrated structure. The temperature-sensitive cell culture device is prepared by this method, and no gap is generated between the culture sheet and the cell culture device, the combination effect is good, and the cell culture device and the culture sheet do not need to be subjected to adhesive or hot-pressing treatment, so that the combination efficiency is high.

The application discloses a micro-fluidic cell chip and a virus separation and culture method based on the cell chip. The micro-fluidic cell chip comprises a metal frame, a lower cover plate, a chip frame, a core chip and an upper cover plate, the core chip is provided with a plurality of cell culture cavities, a main flow channel is arranged on the surface of the core chip, and the main flow channel is communicated with the plurality of cell culture cavities. The virus separation and culture method based on the micro-fluidic cell chip adopts a plurality of cell lines for co-culture, realizes the sequential incubation of one sample to be analyzed and different cell lines, can significantly save clinical samples, maximally improves sample utilization, simultaneously reduces the consumption of culture reagents, shortens the separation and identification time, and theoretically realizes the culture of at least ten different cell lines at the same time due to the design of the micro-fluidic cell chip provided by the application, significantly increases the throughput of virus sensitive host cell screening, and realizes the high-throughput, automatic and rapid screening of virus sensitive cell lines.

An apparatus for culturing cells in connection with a fluid is provided. The apparatus includes a bioreactor including a bed for culturing cells, an agitator for circulating the fluid within the bioreactor, and a controller adapted to control the agitator to operate at a first speed during a first predetermined amount of time and a second speed thereafter. A bioreactor having a fixed bed including a coating to promote cell adhesion and growth is also provided. Related methods are also disclosed.

The invention discloses a pH-sensitive cell membrane liposome fused drug-loaded nano-particle CCM-LP-coated crown-ether as well as a preparation method and application thereof. The pH-sensitive cell membrane liposome fused drug-loaded nano-particle CCM-LP-coated crown-ether is used for targeted regulation of a lung cancer immune microenvironment. The nanoparticle is composed of crown ether, a pH sensitive liposome and a tumor cell membrane, the size is 120 + / -41.2 nm, and surface negative charges of-42.8 + / -5.88 mV are beneficial to prolonging the circulation time, enhancing permeability and retention effect and enhancing accumulation in tumors. The preparation method of the nano-particles comprises the following steps: extracting tumor cell membranes, synthesizing pH-sensitive lipidosome, wrapping drugs, performing mixed ultrasonic treatment and performing dialysis. The CCM-LP (at) crown-ether prepared by the invention has the following advantages: the CCM-LP (at) crown-ether is sensitive to pH and can be used for targeting and specifically removing potassium ions in tumors; m2-type macrophages are induced to be polarized to M1-type macrophages, so that the in-vitro phagocytosis and apoptosis induction capabilities of the macrophages are improved; the biological safety is good; intravenous injection can increase the proportion of M1 / M2, reduce bone marrow-derived suppressor cell infiltration, promote the function and number of CD8 + T cells, recover anti-tumor immunity and effectively inhibit tumor growth.

The invention provides a bioreactor. The bioreactor comprises a tank body assembly; at least one lifting stirring device is arranged on the tank body assembly; the lifting stirring device comprises a mounting frame, a stirring device and a stirring device, wherein the mounting frame is arranged on the tank body assembly; the stirring assembly is arranged on the mounting frame, and the stirring end of the stirring assembly extends into the tank body assembly. According to the invention, traditional rotary stirring is replaced by lifting type stirring, and the stirring disc passively rotates in liquid flow, so that a strong shear field generated by high-speed mechanical stirring is avoided. The inclined hole design enables liquid flow to smoothly pass through, further disperses energy, is especially suitable for cells sensitive to shearing, can reduce cell death or morphological damage caused by shearing, and improves product yield.

The application discloses a photosensitive cell trigger type waterproof GPS positioning device for marine measurement, which comprises a floating bed, a mounting table is arranged at the top of the floating bed, and the mounting table is connected with the floating bed through a damping assembly. The device is floated on the sea surface through the floating bed, the damping assembly is added to reduce the amplitude of excessive vibration of the device caused by sea surface fluctuation, the mounting table is more stable, the components on the device are further protected, the angle of the photosensitive cell panel is changed through the adjusting mechanism, the sunlight can always irradiate the light surface of the photosensitive cell panel, the working efficiency of the photosensitive cell panel is improved, the storage of electric quantity is facilitated through the storage battery, the GPS locator in the device cannot work due to the fact that the photosensitive cell panel cannot work on cloudy days, the GPS locator is installed and fixed through the clamping assembly, and the GPS locator is further waterproofed through the waterproof shell.

In order to improve imaging, in particular for low light intensities, an image recording device, in particular formed as an endoscope, is proposed, which comprises a single photon sensitive detector (SPSD) in addition to an image sensor, which uses photodiodes as light-sensitive cells, in order to respectively detect light from a common object area. With the help of the SPSD, additional image information can be obtained from the object area, to improve the image data recorded with the image sensor or to enhance it with additional image information, in particular with regard to a further spectral range, which is captured with the SPSD.

This invention belongs to the field of fish disease resistance gene technology / virology, specifically involving the application of Endoglin as a receptor for grass carp reovirus type II. The in vitro purified recombinant structural proteins VP4 and VP56 of GCRV-II can specifically bind to Endoglin protein; knockdown in sensitive cell lines and overexpression in insensitive cell lines... Endoglin Genes can affect viral load. Furthermore, using rare gudgeon as a model, gene editing technology was employed to... Endoglin Gene knockout was performed, and the results showed that heterozygotes with the knockout of this host protein significantly inhibited GCRV-II infection in rare gudgeon. These results demonstrate that Endoglin is a key receptor for GCRV-II virus infection in grass carp, providing a foundation for disease-resistant breeding in grass carp and offering important reference value for disease-resistant breeding in other farmed fish species.

This invention discloses a novel use of androstrol-4,6,8(9),13(14)-tetraen-3,11,16-trione, namely its application as a fluorescence imaging reagent. The androstrol-4,6,8(9),13(14)-tetraen-3,11,16-trione solution was co-incubated with human epidermal cells (HaCat) and acute lymphoblastic leukemia cells (BALL-1), respectively, achieving real-time, high-resolution fluorescence imaging of normal and tumor cells. This invention also achieved rapid, real-time in vivo fluorescence imaging in Balb / c nude mice. The compound of this invention is an androstrol natural product derived from *Smilax china*. Its application in cell fluorescence imaging, bioanalysis, and other fields has advantages such as rapid and sensitive cell staining, low cytotoxicity, strong fluorescence signal, and in vivo imaging capabilities, demonstrating practical application value.

A digital radiographic detector includes a planar multilayer core having a two-dimensional array of photo-sensitive cells. An enclosure comprises first and second shells connected together using a rail configured to be secured to at least one of the first and second shells. The rail is attached to at least one of the shells using screws.

The utility model relates to a cell concentration device, and the rotating assembly drives the rotation of the horizontally-arranged rotating support through a driving unit, the centrifugal assembly comprises a centrifugal main pipe penetrating the rotating support and first and second centrifugal branch pipes built-in, and the first and second pump bodies control the feed flow rate and the discharge flow rate respectively, the first and second centrifugal branch pipes are fixed by the rotatable centrifugal main pipe, continuous feeding and discharging are realized, the problem that the traditional intermittent centrifugation must stop fluid operation is solved, the horizontal rotating frame is adopted to cooperate with independent flow rate control, the damage of shear force to sensitive cells is effectively reduced, the double-pump cooperative control of the first and second pump bodies can realize dynamic adjustment of the concentration multiple, and the requirement of different application scenarios is met.

The application discloses a GPX4 protein degrading agent based on a hydrophobic label, a preparation method and application thereof, and belongs to the technical field of medicines, wherein a structural general formula of the GPX4 protein degrading agent based on the hydrophobic label and pharmaceutically acceptable salts thereof is shown as (I). Pharmacological experiments show that the compound has strong anti-proliferation activity on ferroptosis-sensitive cells HT1080, and can effectively down-regulate the GPX4 protein level in the HT1080 cells. The compound has application value of the GPX4 degrading agent, can be applied to preparation of an anti-tumor drug, and has a good market application prospect.

The utility model relates to fermentation tank technical field especially a kind of micro-bubble gas-lifting type fermentation tank without stirring paddle, it includes: tank body and the flow guide pipe that with the tank body form liquid communication;The flow guide pipe is connected by connecting assembly with the tank body, air inlet pipe is placed in the inside of the flow guide pipe, the upper end of the air inlet pipe extends to the top outside of the tank body, and is connected gas source;Wherein, the air inlet pipe is also installed with micro-bubble generator at the inner end of the flow guide pipe, the fermentation tank has no high-speed rotating mechanical component, fluid motion relies on the mild gas lift to drive circulation, and the main shear source is bubble tail vortex and liquid surface bubble breakage, but its intensity is far lower than the shear generated by stirring paddle, suitable for shear sensitive cell, such as mammalian cell, hybridoma cell, stem cell, insect cell, certain fragile microorganism and plant cell culture.

The application belongs to the technical field of fish disease resistance genes or the field of viruses, and discloses application of Tie1 in serving as a receptor of grass carp reovirus type II. The applicant finds that knocking down and overexpressing a Tie1 gene in a sensitive cell line affects the load of grass carp reovirus type II. Experiments prove that fish Tie1 has the functions of promoting virus adhesion and invasion, and knocking down the gene can effectively reduce virus adhesion to host cells and inhibit GCRV-II infection. Therefore, the Tie1 gene can serve as a new target for preventing and treating GCRV-II infection, be used for preparing a preparation for preventing or treating anti-GCRV-II infection, or be used for a cell model for resisting GCRV-II infection or gene editing of a new grass carp variety.

The invention belongs to the technical field of biology, and discloses an influenza virus sensitizing cell strain as well as a construction method and application thereof. The influenza virus sensibilization cell strain STX-TMP2 can stably express a human-derived influenza virus receptor ST6GAL1 and utilizes doxycycline to controllably induce a TMPRSS2 protein. The invention also discloses a preparation method of the influenza virus sensibilization cell strain STX-TMP2. The preservation number of the cell strain STX-TMP2 is GDMCC (China General Microbiological Culture Collection Center) NO: 67099; the cell strain has the following advantages: low toxicity: low background expression is realized through a Tet-on system, cytotoxicity caused by continuous high expression is significantly reduced, and long-term stable passage of the cell strain is ensured; controllability: the doxycycline can regulate and control the expression level and flexibly adapt to the production requirements of influenza virus vaccine strains; high efficiency: in an induction state, the cells have enough receptor expression and serine protease activity at the same time, and the amplification efficiency of the codon reprogramming virus with relatively low replication capacity is remarkably improved; wide applicability: the method is not only suitable for production of influenza virus vaccine strains with codon reprogramming, but also can be popularized to various production systems of attenuated influenza vaccines with relatively low replication ability.

The invention discloses a pharmaceutical composition for enhancing the immunotherapy effect of colon cancer, and relates to the field of biological medicines. The engineered exosome is co-modified by iRGD peptide and pH-sensitive cell-penetrating peptide, mannose molecules and granulocyte-macrophage colony stimulating factors are displayed on the membrane surface of the human umbilical cord blood mesenchymal stem cell-derived exosome, and circ-STAG3L3 specific small interfering RNA, indoleamine 2, 3, 4, 5-tetrahydro-3H-indoleamine 2, 4-tetrahydro-3H-indoleamine 2, 4-tetrahydro-3H-indoleamine 2, 4-tetrahydro-3H-indoleamine 2, 4-tetrahydro-3H-indoleamine 2, 4-tetrahydro-3H-indoleamine 2, 4-tetrahydro-3H-indoleamine 2, 4- a 1, 3-dioxygenase 1 inhibitor and a mitochondrial function enhancer; according to the pharmaceutical composition, siRNA of targeting circ-STAG3L3, an IDO1 inhibitor and a mitochondrial function enhancer are jointly carried in an intelligent engineering exosome, so that a core immunosuppressive factor is effectively knocked down, more importantly, a compensatory immune escape pathway possibly activated by a tumor microenvironment is synchronously blocked, and the mitochondrial function of depleted CD8 + T cells is directly repaired.

An apparatus for culturing cells in connection with a fluid is provided. The apparatus includes a bioreactor including a bed for culturing cells, an agitator for circulating the fluid within the bioreactor, and a controller adapted to control the agitator to operate at a first speed during a first predetermined amount of time and a second speed thereafter. A bioreactor having a fixed bed including a coating to promote cell adhesion and growth is also provided. Related methods are also disclosed.