71 results about "Sensitive cell" patented technology

Each smallest repeating unit (SRU) in a colour filter array has a first sub-unit (FSU) and a second sub-unit (SSU). For a first sub-image, first image data is read out from photo-sensitive cells that correspond to FSUs of SRUs lying in a row (R1-R3) or column, and by skipping reading out from photo-sensitive cells that correspond to SSUs of a first predefined percent (FPP) of the SRUs, while reading out the first image data from photo-sensitive cells that correspond to SSUs of a remainder of the SRUs. For a second sub-image, second image data is read out from photo-sensitive cells that correspond to FSUs of the FPP, whilst skipping reading out from photo-sensitive cells that correspond to FSUs of the remainder, and by skipping reading out from photo-sensitive cells that correspond to the SSUs of the SRUs. Sub-pixel shift is performed between the sub-images. Image(s) are then generated from the sub-images.

The invention provides an uncooled infrared detector reading circuit, an infrared detector and a control method of the infrared detector. The control method is applied to the uncooled infrared detector, the uncooled infrared detector comprises an uncooled infrared focal plane array with multiple rows and columns of infrared sensitive pixels, and the control method comprises the following steps: controlling the uncooled infrared detector to execute a first working state; in the first working state, part of the infrared sensitive pixels are gated so that signals can be read out; controlling the non-refrigeration infrared detector to be switched to a second working state; in the second working state, at least part of the infrared sensitive pixels are gated so that signals can be read out; wherein the number of the infrared sensitive pixels gated in the second working state is greater than the number of the infrared sensitive pixels gated in the first working state.

The invention provides a visual stimulation method and device based on non-imaging visual response, equipment and a program product, and relates to the technical field of electronics. Visual stimulation signals capable of triggering non-visual light stimulation are creatively applied to two visual channels of a user respectively in a time asynchronous mode. The visual channel which preferentially senses the stimulation signal enables the stimulation signal to directly stimulate the corresponding sensing channel through the photosensitive sensing cell due to non-imaging visual response. The image content of the stimulation signal needs to be perceived by the brain only after the stimulation signal is received in the other visual channel. Therefore, based on the visual stimulation method, the non-visual light stimulation and the visual image stimulation generated by the light stimulation signal are not synchronous in time, so that the mutual influence of the two processes is reduced, the stimulation effect of the non-visual light stimulation is improved, and the shaping capability of the non-visual light stimulation on a corresponding perception path is enhanced.

Disclosed is an imaging system with image sensor and processor(s). The image sensor has a plurality of photo-sensitive cells and a colour filter array. The processor(s) is configured to read out unprocessed colour data from those photo-sensitive cells that correspond to the colour filters of the at least three different colours; generate coarse depth data by processing data read out from at least one of: those photo-sensitive cells that correspond to the colour filters that allow the at least one infrared wavelength to pass through the PDAF photo-sensitive cells; perform interpolation and demosaicking on the unprocessed colour data and the coarse depth data, using at least one neural network, to generate a full-resolution colour data and a full-resolution depth data; and reproject the full-resolution colour data from a given camera pose to a given eye pose of a user, by utilising the full-resolution depth data.

Disclosed is an imaging system including an image sensor with a plurality of photo-sensitive cells arranged on a photo-sensitive surface of the image sensor; a light valve device with an array of light valves, wherein the array of light valves is arranged on an optical path of the photo-sensitive surface of the image sensor; and at least one processor configured to: control an exposure of photo-sensitive cells of at least a first region in the photo-sensitive surface by utilising respective light valves of said array; read out image data from the photo-sensitive cells of the image sensor; and process the image data to generate an image.

A chromatic confocal measuring device for measuring the distance to an object (40; 50) contains a spectrograph (46) which converts charge carriers released in light-sensitive cells (49; 49') and having two different amplifications into electric voltages (V1, V2) such that two voltages are output (V1, V2) each time a light-sensitive cell (49; 49') is exposed. An evaluation unit (30) is configured to calculate a distance value for each exposure cycle taking into consideration the two voltages (V1, V2) generated by the light-sensitive cells (49; 49').

Image data from an image sensor is read out, wherein when reading out, processor(s) is / are configured to employ subsampling by: reading out the image data from photo-sensitive cells that correspond to a first set of lines in a colour filter array (CFA), wherein a given line in the first set has colour filters of each of at least three different colours, the given line being a row (R1-R8) or a column (C1-C8) of the CFA; and skipping read out from photo-sensitive cells that correspond to a second set of lines in the CFA, wherein the first set of lines has one of: odd lines in the CFA, even lines in the CFA, while the second set of lines has another of: the odd lines, the even lines. The image data is processed to generate an image.

The invention relates to a novel ferrocenyl hydrophobic label degradation agent targeting GPX4 as well as a preparation method and application of the novel ferrocenyl hydrophobic label degradation agent. The GPX4 protein degradation agent has a chemical structure as shown in a formula (I). Pharmacological experiments show that the derivative or salt disclosed by the invention can be used for remarkably degrading GPX4 protein in ferroptosis sensitive cells (such as HT1080, OS-RC-2, 4T1 and 786-O), and has very strong anti-proliferative activity on the four cell strains. The invention also provides a preparation method of the derivative and the pharmaceutically acceptable salt thereof, and an application of the derivative and the pharmaceutically acceptable salt thereof as a GPX4 degradation agent. Formula (I).

The present invention relates to a proficiency testing article for Chlamydia trachomatis detection, its preparation method, and laboratory application. The proficiency testing article is obtained by culturing CT serotype E in a CT-sensitive cell line and includes a lyophilized preparation and a reconstituted solution. The article simulates clinical CT samples and serves as a proficiency testing article for clinical CT infection diagnosis using colloidal gold, latex, and nucleic acid detection methods. The article is used for external quality assessment, internal quality control, and kit performance evaluation in clinical laboratories.

Disclosed is system with imaging unit(s) and processor(s), imaging unit(s) with image sensor of photo-sensitive cells; and light valve device having array of light valves. The processor(s) is configured to: control light valves to allow light to pass therethrough, while controlling remaining light valves in the array to stop allowing the light to pass therethrough, thereby casting shadows of remaining light valves on first part and second part in first set and second set of photo-sensitive cells, respectively; read out image signals from photo-sensitive cells; select pairs of photo-sensitive cells from first set and second set; for given pair, determine phase difference between image signals read out from photo-sensitive cells of first set and second set; and adjust focus distance of the imaging unit(s), based on phase differences.

The invention belongs to the technical field of biology, and particularly relates to preparation of DMSO-free cryopreservation liquid and application of the DMSO-free cryopreservation liquid in cell cryopreservation. The invention provides a DMSO (dimethyl sulfoxide)-free serum-free cell cryopreservation solution. Each 100 mL of the cell cryopreservation solution contains 1-100 mg of thermo-sensitive nanogel, 0.1-10 mg of trehalose, 10-100 [mu] L of glycerol and a basic culture medium. The nanogel (preferably pNIPAM) forms a protective layer at low temperature to inhibit ice crystal damage, and is rapidly dissociated during rewarming; and trehalose-glycerol synergistically stabilizes cell membranes. The cryopreservation liquid does not need programmed cooling, does not need centrifugation after resuscitation, is suitable for sensitive cells such as CAR-T cells and stem cells, has the resuscitation survival rate of more than 90%, and completely avoids the toxicity of DMSO and the risk of serum pollution.

The invention relates to an ultraviolet damage sensitive cell model as well as a preparation method and application thereof. The cell model is a cell with UVSSA gene function deletion. According to the invention, UVSSA genes in human epidermal Hacat cells are knocked out by utilizing a CRISPR-Cas9 gene editing technology, and a cell model sensitive to medium-wave-band ultraviolet (UVB) is constructed and is successfully used for screening active raw materials with a light protection effect.

The application discloses a PIP-seq technology-based adjustable osmotic pressure single-cell sequencing kit and a detection method, and through a wrapping buffer with adjustable osmotic pressure and a non-toxic digestion system, high-throughput sequencing of marine high-osmotic living cells and freshwater low-osmotic living cells without chemical fixation is realized for the first time, and the retention rate of sensitive cell groups (such as stinging cells and freshwater ion-regulating cells) is doubled. By only changing the buffer formula, marine and freshwater samples can be seamlessly switched without changing the core reaction process, and the kit has strong versatility, and successfully solves the osmotic pressure barrier problem that has long plagued the field of aquatic biology.

As botulinum toxin has recently been attempted as a therapeutic agent for various indications, the demand for botulinum toxin in the medical and cosmetic fields has rapidly increased. However, there is no stable and highly reproducible cell-based assay method for measuring the potency of botulinum toxin. Since botulinum toxin is a very potent neurotoxin protein, the development of highly specific and sensitive cells is particularly required for accurate cell-based measurement of the potency of botulinum toxin. The present invention is directed to a neuronal cell line with altered gene expression. The transformed cell line according to the present invention has significantly increased sensitivity to botulinum toxin, and thus is expected to be very effectively used for cell-based determination of botulinum toxin activity or cell-based detection of botulinum toxin.

A chromatic-confocal measuring device for measuring the distance to an object (40; 50) contains a spectrograph (46) that converts charge carriers released in light-sensitive cells (49; 49') into electrical voltages (V1, V2) with two different amplifications, so that two voltages (V1, V2) are output for each exposure of a light-sensitive cell (49; 49'). An evaluation unit (30) is configured to calculate a distance value for each exposure cycle, taking into account the two voltages (V1, V2) generated by the light-sensitive cells (49; 49').

A functionalized object includes at least one mechanical wave sensor providing the object with a vibration and deformation detection capability. The mechanical wave sensor comprises: a sensitive cell having a thickness less than or equal to 50 microns, and comprising an active layer made of a monocrystalline or polycrystalline piezoelectric material and two electrodes, which are in contact with the active layer and accessible at a first surface of the sensitive cell, and a support layer secured to the second surface of the sensitive cell and secured to the object. The functionalized object comprises at least two electrically conductive strips disposed on the first surface of the sensitive cell and on a surface of the object, each strip connecting an electrode to an electrical contact pad. A method is used for producing such a functionalized object.

The application belongs to the technical field of cell culture, and particularly relates to a temperature-sensitive cell three-dimensional culture support, which comprises temperature-sensitive hydroxypropyl chitin, the molecular weight of the temperature-sensitive hydroxypropyl chitin is 15,000-300,000 Da, and the degree of deacetylation (DD) is 35%-65%. The support material is temperature-sensitive hydroxypropyl chitin prepared by derivatization of chitosan of a natural source through a specific process, and can be used for three-dimensional culture of cells. The temperature-sensitive hydroxypropyl chitin is used for three-dimensional culture of cells, and can significantly reduce the culture cost, improve the convenience and the viability of the cultured cells.

This provides a continuous culture process for culturing populations of shear-sensitive cells. [Solution] A continuous culture process for culturing a population of cells consisting of shear-sensitive cells, comprising controlling the gas outflow rate of a bioreactor system so that the gas outflow rate does not exceed 20 m / s, wherein the bioreactor system includes at least 25 L of culture medium, and the population of cells is 20 × 10 6 From cells / mL to 15 × 10 7 The process is characterized by achieving a steady-state viable cell concentration within the range of cells / mL, and the gas efflux rate being controlled at least until the bioreactor system reaches steady-state conditions.

Disclosed is system with imaging unit(s) and processor(s), imaging unit(s) with image sensor of photo-sensitive cells; and light valve device having array of light valves. The processor(s) is configured to: control light valves to allow light to pass therethrough, while controlling remaining light valves in the array to stop allowing the light to pass therethrough, thereby casting shadows of remaining light valves on first part and second part in first set and second set of photo-sensitive cells, respectively; read out image signals from photo-sensitive cells; select pairs of photo-sensitive cells from first set and second set; for given pair, determine phase difference between image signals read out from photo-sensitive cells of first set and second set; and adjust focus distance of the imaging unit(s), based on phase differences.

Disclosed is an imaging system including an image sensor with a plurality of photo-sensitive cells arranged on a photo-sensitive surface of the image sensor; a light valve device with an array of light valves, wherein the array of light valves is arranged on an optical path of the photo-sensitive surface of the image sensor; and at least one processor configured to: control an exposure of photo-sensitive cells of at least a first region in the photo-sensitive surface by utilising respective light valves of said array; read out image data from the photo-sensitive cells of the image sensor; and process the image data to generate an image.

The invention discloses a hydroxybutyl / N-carboxymethyl chitosan derivative as well as a preparation method and application thereof, and belongs to the field of biomedical materials. The chitosan derivative can be obtained by firstly carrying out carboxymethylation modification on chitosan and then carrying out hydroxybutylation modification. Or the chitosan is firstly subjected to hydroxybutylation modification and then subjected to carboxymethyl modification. The hydroxybutyl / N-carboxymethyl chitosan derivative disclosed by the invention can be compatible with an osmotic pressure regulator of a strong-polarity ionic compound to obtain a temperature-sensitive solution with good performance, the solution is uniform and transparent at low temperature, the gel is also uniform at body temperature, the transparency is very high, the gel strength is relatively large, the problems of whitening and fragmentation do not exist, and the application prospect is wide. And the method can be well matched with the human physiological environment. The material can be used as a temperature-sensitive cell carrier, a tissue engineering scaffold material, a drug controlled release material and a body surface superficial layer and deep layer tissue or in-vivo implant material.

The invention discloses a carboxyethyl hydroxybutyl chitosan derivative as well as a preparation method and application thereof, and belongs to the field of biomedical materials. The preparation method comprises the following steps: performing carboxyethylation modification on chitosan to obtain carboxyethyl chitosan, and performing hydroxybutylation modification to obtain the carboxyethyl chitosan; or the chitosan is firstly subjected to hydroxybutylation modification to obtain hydroxybutyl chitosan, and then the hydroxybutyl chitosan is subjected to carboxyethyl group modification to obtain the product; or chitosan is subjected to hydroxybutylation and carboxyethylation modification at the same time to obtain the product. The carboxyethyl hydroxybutyl derivative disclosed by the invention can be compatible with an osmotic pressure regulator of an ionic compound with strong polarity to obtain a temperature-sensitive solution with good performance, the solution is uniform and transparent at low temperature, the gel is also uniform at body temperature, the transparency is very high, the gel strength is relatively large, the problems of whitening and fragmentation do not exist, and the stability is good. And the method can be well matched with the human physiological environment. The material can be used as a temperature-sensitive cell carrier, a tissue engineering scaffold material and a superficial and deep body surface tissue or in-vivo implant material.

The invention belongs to the technical field of biology, and discloses an influenza B virus sensitizing cell strain as well as a construction method and application thereof. The influenza B virus sensitizing cell strain STX-BB2 can be used for stably expressing influenza virus receptor ST6GAL1 protein in a human source and controllably inducing and expressing influenza B virus PB2 protein by using doxycycline; the preservation number of the cell strain STX-BB2 is GDMCC (China General Microbiological Culture Collection Center) NO: 67100; the cell strain can enhance the expression ability of a host receptor and the virus replication key gene level at the same time; the infection efficiency and the replication titer of the influenza B virus in MDCK cells can be improved; the method can be widely applied to influenza B virus separation, mechanism research, vaccine seed virus amplification and drug screening model construction.

The invention provides a stable passage cell line SSFin cell derived from fin ray tissue of sebastes schlegeli, and the stable passage cell line SSFin cell can be used for related research on disease-resistant immunity of sebastes schlegeli. The preservation number of the sebastes schlegeli fin ray tissue cell line SSFin provided by the invention is CCTCC (China Center For Type Culture Collection) NO: C202371. The stable passage cell line derived from fin ray tissue of sebastes schlegeli provided by the invention has sensitivity to pathogenic bacteria infection, and can be used as a sensitive cell for aquatic etiology and immunology research.

According to one embodiment, a nucleic acid construct is used for detecting an estrogen-sensitive cell. The nucleic acid construct includes an enhancer sequence, a promoter sequence and a reporter gene. The enhancer sequence includes at least an ERE sequence and at least a XRE sequence. The promoter sequence is ligated to downstream of the enhancer sequence. The reporter gene is ligated to downstream of the promoter sequence.

The present disclosure provides, among other things, continuous culture methods for producing a cell product, e.g., a recombinant protein, e.g., a glycoprotein, e.g., an antibody agent or a fusion protein. In some instances, methods herein allow large-scale production of a recombinant protein using continuous culture methods. The present disclosure identifies and addresses a problem with current continuous cell culture techniques in that at large-scale culture of certain cells have insufficient viable cell concentrations and impaired cell viability. The present disclosure provides, in part, methods and systems for large-scale continuous culture of shear-sensitive cells.

The invention discloses a chlorinated trimethyl amino hydroxypropyl hydroxybutyl chitosan derivative as well as a preparation method and application thereof, and belongs to the field of biomedical materials. According to the preparation method, chitosan is subjected to trimethyl amino hydroxypropyl group chloride modification so as to obtain trimethyl amino hydroxypropyl chitosan chloride, and then hydroxybutylation modification is carried out so as to obtain the modified chitosan; or the chitosan is firstly subjected to hydroxybutylation modification and then subjected to chlorinated trimethylamino hydroxypropyl group modification, and the chitosan is obtained; or chitosan is subjected to hydroxybutyl and chlorinated trimethylamino hydroxypropyl group modification at the same time to obtain the product. The chitosan derivative disclosed by the invention is a positively charged high-molecular polymer and non-amphoteric polyelectrolyte, does not have a self-compounding phenomenon caused by positive and negative charge attraction, and can be compatible with an osmotic pressure regulator of a strong-polarity ionic compound to obtain a temperature-sensitive solution with good performance. The material can be used as a temperature-sensitive cell carrier, a tissue engineering scaffold material, a drug controlled release material and a body surface superficial layer and deep layer tissue or in-vivo implant material.

This invention relates to the field of biomedicine, specifically to a synergistic and targeted epigenetic pharmaceutical composition for the treatment of lung cancer. In studies of drug-resistant lung cancer cells, it was found that overexpression of the NSUN7 gene in drug-resistant lung cancer cells can inhibit MZF1. L The expression of MZF1 also promotes S When the expression of NSUN7 gene was suppressed, no promotion of MZF1 was found. L The expression of MZF1 can be suppressed at the same time. S The expression of NSUN7 gene was inhibited, meaning no opposite result was obtained. However, when NSUN7 gene expression was inhibited and EGFR-TKI inhibitors were administered to drug-resistant lung cancer cells, MZF1 cells in drug-resistant lung cancer cells showed a positive result. L Upregulation, MZF1 S Decreased expression of NSUN7 leads to the transformation of drug-resistant lung cancer cells into drug-sensitive cells. Therefore, interfering with NSUN7 in combination with epigenetic drugs to regulate the alternative splicing of methylation-sensitive transcription factors can increase the specificity of regulatory targets and synergistically enhance the in vitro and in vivo antitumor effects.

The utility model relates to the technical field of cell culture, in particular to a cell fluid infusion device which comprises a working table, a mounting table is fixed on the top surface of the working table, and a heat preservation device is fixed at the top of the mounting table. According to the utility model, the loss of fluid infusion temperature can be reduced, the stability of cell culture is facilitated, and the accuracy of experiments, especially the culture experiments of temperature-sensitive cells, is ensured.

The present invention relates to a cell for measuring botulinum toxin activity that overexpresses SEPTIN2 or Thioredoxin (TXN), and a method for measuring botulinum toxin activity, comprising the steps of treating the cell for measuring botulinum toxin activity with botulinum toxin and culturing it, lysing the cultured cells to collect a cell lysate, and measuring the amount of SNAP-25 cleavage products in the cell lysate.