52 results about "Sensitive cell" patented technology

Each smallest repeating unit (SRU) in a colour filter array has a first sub-unit (FSU) and a second sub-unit (SSU). For a first sub-image, first image data is read out from photo-sensitive cells that correspond to FSUs of SRUs lying in a row (R1-R3) or column, and by skipping reading out from photo-sensitive cells that correspond to SSUs of a first predefined percent (FPP) of the SRUs, while reading out the first image data from photo-sensitive cells that correspond to SSUs of a remainder of the SRUs. For a second sub-image, second image data is read out from photo-sensitive cells that correspond to FSUs of the FPP, whilst skipping reading out from photo-sensitive cells that correspond to FSUs of the remainder, and by skipping reading out from photo-sensitive cells that correspond to the SSUs of the SRUs. Sub-pixel shift is performed between the sub-images. Image(s) are then generated from the sub-images.

The invention provides a visual stimulation method and device based on non-imaging visual response, equipment and a program product, and relates to the technical field of electronics. Visual stimulation signals capable of triggering non-visual light stimulation are creatively applied to two visual channels of a user respectively in a time asynchronous mode. The visual channel which preferentially senses the stimulation signal enables the stimulation signal to directly stimulate the corresponding sensing channel through the photosensitive sensing cell due to non-imaging visual response. The image content of the stimulation signal needs to be perceived by the brain only after the stimulation signal is received in the other visual channel. Therefore, based on the visual stimulation method, the non-visual light stimulation and the visual image stimulation generated by the light stimulation signal are not synchronous in time, so that the mutual influence of the two processes is reduced, the stimulation effect of the non-visual light stimulation is improved, and the shaping capability of the non-visual light stimulation on a corresponding perception path is enhanced.

Disclosed is an imaging system with image sensor and processor(s). The image sensor has a plurality of photo-sensitive cells and a colour filter array. The processor(s) is configured to read out unprocessed colour data from those photo-sensitive cells that correspond to the colour filters of the at least three different colours; generate coarse depth data by processing data read out from at least one of: those photo-sensitive cells that correspond to the colour filters that allow the at least one infrared wavelength to pass through the PDAF photo-sensitive cells; perform interpolation and demosaicking on the unprocessed colour data and the coarse depth data, using at least one neural network, to generate a full-resolution colour data and a full-resolution depth data; and reproject the full-resolution colour data from a given camera pose to a given eye pose of a user, by utilising the full-resolution depth data.

Disclosed is an imaging system including an image sensor with a plurality of photo-sensitive cells arranged on a photo-sensitive surface of the image sensor; a light valve device with an array of light valves, wherein the array of light valves is arranged on an optical path of the photo-sensitive surface of the image sensor; and at least one processor configured to: control an exposure of photo-sensitive cells of at least a first region in the photo-sensitive surface by utilising respective light valves of said array; read out image data from the photo-sensitive cells of the image sensor; and process the image data to generate an image.

A chromatic confocal measuring device for measuring the distance to an object (40; 50) contains a spectrograph (46) which converts charge carriers released in light-sensitive cells (49; 49') and having two different amplifications into electric voltages (V1, V2) such that two voltages are output (V1, V2) each time a light-sensitive cell (49; 49') is exposed. An evaluation unit (30) is configured to calculate a distance value for each exposure cycle taking into consideration the two voltages (V1, V2) generated by the light-sensitive cells (49; 49').

Image data from an image sensor is read out, wherein when reading out, processor(s) is / are configured to employ subsampling by: reading out the image data from photo-sensitive cells that correspond to a first set of lines in a colour filter array (CFA), wherein a given line in the first set has colour filters of each of at least three different colours, the given line being a row (R1-R8) or a column (C1-C8) of the CFA; and skipping read out from photo-sensitive cells that correspond to a second set of lines in the CFA, wherein the first set of lines has one of: odd lines in the CFA, even lines in the CFA, while the second set of lines has another of: the odd lines, the even lines. The image data is processed to generate an image.

The invention relates to a novel ferrocenyl hydrophobic label degradation agent targeting GPX4 as well as a preparation method and application of the novel ferrocenyl hydrophobic label degradation agent. The GPX4 protein degradation agent has a chemical structure as shown in a formula (I). Pharmacological experiments show that the derivative or salt disclosed by the invention can be used for remarkably degrading GPX4 protein in ferroptosis sensitive cells (such as HT1080, OS-RC-2, 4T1 and 786-O), and has very strong anti-proliferative activity on the four cell strains. The invention also provides a preparation method of the derivative and the pharmaceutically acceptable salt thereof, and an application of the derivative and the pharmaceutically acceptable salt thereof as a GPX4 degradation agent. Formula (I).

The present invention relates to a proficiency testing article for Chlamydia trachomatis detection, its preparation method, and laboratory application. The proficiency testing article is obtained by culturing CT serotype E in a CT-sensitive cell line and includes a lyophilized preparation and a reconstituted solution. The article simulates clinical CT samples and serves as a proficiency testing article for clinical CT infection diagnosis using colloidal gold, latex, and nucleic acid detection methods. The article is used for external quality assessment, internal quality control, and kit performance evaluation in clinical laboratories.

Disclosed is system with imaging unit(s) and processor(s), imaging unit(s) with image sensor of photo-sensitive cells; and light valve device having array of light valves. The processor(s) is configured to: control light valves to allow light to pass therethrough, while controlling remaining light valves in the array to stop allowing the light to pass therethrough, thereby casting shadows of remaining light valves on first part and second part in first set and second set of photo-sensitive cells, respectively; read out image signals from photo-sensitive cells; select pairs of photo-sensitive cells from first set and second set; for given pair, determine phase difference between image signals read out from photo-sensitive cells of first set and second set; and adjust focus distance of the imaging unit(s), based on phase differences.

The invention belongs to the technical field of biology, and particularly relates to preparation of DMSO-free cryopreservation liquid and application of the DMSO-free cryopreservation liquid in cell cryopreservation. The invention provides a DMSO (dimethyl sulfoxide)-free serum-free cell cryopreservation solution. Each 100 mL of the cell cryopreservation solution contains 1-100 mg of thermo-sensitive nanogel, 0.1-10 mg of trehalose, 10-100 [mu] L of glycerol and a basic culture medium. The nanogel (preferably pNIPAM) forms a protective layer at low temperature to inhibit ice crystal damage, and is rapidly dissociated during rewarming; and trehalose-glycerol synergistically stabilizes cell membranes. The cryopreservation liquid does not need programmed cooling, does not need centrifugation after resuscitation, is suitable for sensitive cells such as CAR-T cells and stem cells, has the resuscitation survival rate of more than 90%, and completely avoids the toxicity of DMSO and the risk of serum pollution.

The application discloses a PIP-seq technology-based adjustable osmotic pressure single-cell sequencing kit and a detection method, and through a wrapping buffer with adjustable osmotic pressure and a non-toxic digestion system, high-throughput sequencing of marine high-osmotic living cells and freshwater low-osmotic living cells without chemical fixation is realized for the first time, and the retention rate of sensitive cell groups (such as stinging cells and freshwater ion-regulating cells) is doubled. By only changing the buffer formula, marine and freshwater samples can be seamlessly switched without changing the core reaction process, and the kit has strong versatility, and successfully solves the osmotic pressure barrier problem that has long plagued the field of aquatic biology.

As botulinum toxin has recently been attempted as a therapeutic agent for various indications, the demand for botulinum toxin in the medical and cosmetic fields has rapidly increased. However, there is no stable and highly reproducible cell-based assay method for measuring the potency of botulinum toxin. Since botulinum toxin is a very potent neurotoxin protein, the development of highly specific and sensitive cells is particularly required for accurate cell-based measurement of the potency of botulinum toxin. The present invention is directed to a neuronal cell line with altered gene expression. The transformed cell line according to the present invention has significantly increased sensitivity to botulinum toxin, and thus is expected to be very effectively used for cell-based determination of botulinum toxin activity or cell-based detection of botulinum toxin.

A chromatic-confocal measuring device for measuring the distance to an object (40; 50) contains a spectrograph (46) that converts charge carriers released in light-sensitive cells (49; 49') into electrical voltages (V1, V2) with two different amplifications, so that two voltages (V1, V2) are output for each exposure of a light-sensitive cell (49; 49'). An evaluation unit (30) is configured to calculate a distance value for each exposure cycle, taking into account the two voltages (V1, V2) generated by the light-sensitive cells (49; 49').

A functionalized object includes at least one mechanical wave sensor providing the object with a vibration and deformation detection capability. The mechanical wave sensor comprises: a sensitive cell having a thickness less than or equal to 50 microns, and comprising an active layer made of a monocrystalline or polycrystalline piezoelectric material and two electrodes, which are in contact with the active layer and accessible at a first surface of the sensitive cell, and a support layer secured to the second surface of the sensitive cell and secured to the object. The functionalized object comprises at least two electrically conductive strips disposed on the first surface of the sensitive cell and on a surface of the object, each strip connecting an electrode to an electrical contact pad. A method is used for producing such a functionalized object.

The application belongs to the technical field of cell culture, and particularly relates to a temperature-sensitive cell three-dimensional culture support, which comprises temperature-sensitive hydroxypropyl chitin, the molecular weight of the temperature-sensitive hydroxypropyl chitin is 15,000-300,000 Da, and the degree of deacetylation (DD) is 35%-65%. The support material is temperature-sensitive hydroxypropyl chitin prepared by derivatization of chitosan of a natural source through a specific process, and can be used for three-dimensional culture of cells. The temperature-sensitive hydroxypropyl chitin is used for three-dimensional culture of cells, and can significantly reduce the culture cost, improve the convenience and the viability of the cultured cells.

This provides a continuous culture process for culturing populations of shear-sensitive cells. [Solution] A continuous culture process for culturing a population of cells consisting of shear-sensitive cells, comprising controlling the gas outflow rate of a bioreactor system so that the gas outflow rate does not exceed 20 m / s, wherein the bioreactor system includes at least 25 L of culture medium, and the population of cells is 20 × 10 6 From cells / mL to 15 × 10 7 The process is characterized by achieving a steady-state viable cell concentration within the range of cells / mL, and the gas efflux rate being controlled at least until the bioreactor system reaches steady-state conditions.

Disclosed is system with imaging unit(s) and processor(s), imaging unit(s) with image sensor of photo-sensitive cells; and light valve device having array of light valves. The processor(s) is configured to: control light valves to allow light to pass therethrough, while controlling remaining light valves in the array to stop allowing the light to pass therethrough, thereby casting shadows of remaining light valves on first part and second part in first set and second set of photo-sensitive cells, respectively; read out image signals from photo-sensitive cells; select pairs of photo-sensitive cells from first set and second set; for given pair, determine phase difference between image signals read out from photo-sensitive cells of first set and second set; and adjust focus distance of the imaging unit(s), based on phase differences.

Disclosed is an imaging system including an image sensor with a plurality of photo-sensitive cells arranged on a photo-sensitive surface of the image sensor; a light valve device with an array of light valves, wherein the array of light valves is arranged on an optical path of the photo-sensitive surface of the image sensor; and at least one processor configured to: control an exposure of photo-sensitive cells of at least a first region in the photo-sensitive surface by utilising respective light valves of said array; read out image data from the photo-sensitive cells of the image sensor; and process the image data to generate an image.

The invention belongs to the technical field of biology, and discloses an influenza B virus sensitizing cell strain as well as a construction method and application thereof. The influenza B virus sensitizing cell strain STX-BB2 can be used for stably expressing influenza virus receptor ST6GAL1 protein in a human source and controllably inducing and expressing influenza B virus PB2 protein by using doxycycline; the preservation number of the cell strain STX-BB2 is GDMCC (China General Microbiological Culture Collection Center) NO: 67100; the cell strain can enhance the expression ability of a host receptor and the virus replication key gene level at the same time; the infection efficiency and the replication titer of the influenza B virus in MDCK cells can be improved; the method can be widely applied to influenza B virus separation, mechanism research, vaccine seed virus amplification and drug screening model construction.

The invention provides a stable passage cell line SSFin cell derived from fin ray tissue of sebastes schlegeli, and the stable passage cell line SSFin cell can be used for related research on disease-resistant immunity of sebastes schlegeli. The preservation number of the sebastes schlegeli fin ray tissue cell line SSFin provided by the invention is CCTCC (China Center For Type Culture Collection) NO: C202371. The stable passage cell line derived from fin ray tissue of sebastes schlegeli provided by the invention has sensitivity to pathogenic bacteria infection, and can be used as a sensitive cell for aquatic etiology and immunology research.

According to one embodiment, a nucleic acid construct is used for detecting an estrogen-sensitive cell. The nucleic acid construct includes an enhancer sequence, a promoter sequence and a reporter gene. The enhancer sequence includes at least an ERE sequence and at least a XRE sequence. The promoter sequence is ligated to downstream of the enhancer sequence. The reporter gene is ligated to downstream of the promoter sequence.

The present disclosure provides, among other things, continuous culture methods for producing a cell product, e.g., a recombinant protein, e.g., a glycoprotein, e.g., an antibody agent or a fusion protein. In some instances, methods herein allow large-scale production of a recombinant protein using continuous culture methods. The present disclosure identifies and addresses a problem with current continuous cell culture techniques in that at large-scale culture of certain cells have insufficient viable cell concentrations and impaired cell viability. The present disclosure provides, in part, methods and systems for large-scale continuous culture of shear-sensitive cells.

This invention relates to the field of biomedicine, specifically to a synergistic and targeted epigenetic pharmaceutical composition for the treatment of lung cancer. In studies of drug-resistant lung cancer cells, it was found that overexpression of the NSUN7 gene in drug-resistant lung cancer cells can inhibit MZF1. L The expression of MZF1 also promotes S When the expression of NSUN7 gene was suppressed, no promotion of MZF1 was found. L The expression of MZF1 can be suppressed at the same time. S The expression of NSUN7 gene was inhibited, meaning no opposite result was obtained. However, when NSUN7 gene expression was inhibited and EGFR-TKI inhibitors were administered to drug-resistant lung cancer cells, MZF1 cells in drug-resistant lung cancer cells showed a positive result. L Upregulation, MZF1 S Decreased expression of NSUN7 leads to the transformation of drug-resistant lung cancer cells into drug-sensitive cells. Therefore, interfering with NSUN7 in combination with epigenetic drugs to regulate the alternative splicing of methylation-sensitive transcription factors can increase the specificity of regulatory targets and synergistically enhance the in vitro and in vivo antitumor effects.

The utility model relates to the technical field of cell culture, in particular to a cell fluid infusion device which comprises a working table, a mounting table is fixed on the top surface of the working table, and a heat preservation device is fixed at the top of the mounting table. According to the utility model, the loss of fluid infusion temperature can be reduced, the stability of cell culture is facilitated, and the accuracy of experiments, especially the culture experiments of temperature-sensitive cells, is ensured.

The present invention relates to a cell for measuring botulinum toxin activity that overexpresses SEPTIN2 or Thioredoxin (TXN), and a method for measuring botulinum toxin activity, comprising the steps of treating the cell for measuring botulinum toxin activity with botulinum toxin and culturing it, lysing the cultured cells to collect a cell lysate, and measuring the amount of SNAP-25 cleavage products in the cell lysate.

The invention provides a tick-borne encephalitis virus monoclonal antibody and application thereof, and relates to the field of biotechnology and virology. A mouse monoclonal antibody mAb-16 with specific neutralizing activity is screened, the monoclonal antibody mAb-16 mainly aims at envelope protein E of tick-borne encephalitis virus, the monoclonal antibody mAb-16 can specifically recognize and neutralize domain III in E protein EDIII of tick-borne encephalitis virus, and a biological membrane layer interference experiment shows that the monoclonal antibody mAb-16 has relatively strong interaction with the domain III of the E protein. The median inhibition concentration of the monoclonal antibody mAb-16 on primary viruses cultured by sensitive cells is 92.80 ng / mL; in a lethal mouse infection model, a high-dose group has complete protection activity and can be used for preparing tick-borne encephalitis virus detection reagents or related drugs for neutralizing and inhibiting tick-borne encephalitis viruses, so that prevention and treatment of tick-borne encephalitis viruses are facilitated.

The present application relates to the technical field of cell culture, and discloses a preparation method of a temperature-sensitive cell culture device, which relates to a mold and a culture sheet. One side of the culture sheet is coated with a temperature-sensitive liquid layer. The mold is provided with a mold cavity, and the mold cavity is provided with a groove for accommodating the culture sheet. The preparation method comprises the following steps: placing the culture sheet in the groove, with the side coated with the temperature-sensitive liquid layer facing the bottom of the groove; injecting a molten material into the mold cavity, and the molten material forms the cell culture device after cooling; and when the cell culture device is formed, the culture sheet is located at the bottom of the concave cavity of the cell culture device and is combined with the cell culture device to form an integrated structure. The temperature-sensitive cell culture device is prepared by this method, and no gap is generated between the culture sheet and the cell culture device, the combination effect is good, and the cell culture device and the culture sheet do not need to be subjected to adhesive or hot-pressing treatment, so that the combination efficiency is high.

The invention discloses an acylated anion group substituted hydroxybutyl chitosan derivative as well as a preparation method and application thereof, and belongs to the field of biomedical materials. The acylated anion group substituted hydroxybutyl chitosan derivative has the following structure: # imgabs0 #, wherein R1, R2, R3 and R4 are one of the following groups: H, an acyl group, hydroxybutyl, polyhydroxybutyl and an anion substituent group. The hydroxybutyl chitosan derivative disclosed by the invention can form a solution with pure water, or is compatible with polyhydric alcohols such as glycerol, mannitol and the like, and even is compatible with a strong-polarity ionic compound, so that a temperature-sensitive solution with good performance can be obtained; the temperature-sensitive cell carrier has good compatibility with a cell culture solution, can enable cells to survive and promote cell proliferation, and is a temperature-sensitive cell carrier and tissue engineering scaffold material with a better effect.

The application discloses a micro-fluidic cell chip and a virus separation and culture method based on the cell chip. The micro-fluidic cell chip comprises a metal frame, a lower cover plate, a chip frame, a core chip and an upper cover plate, the core chip is provided with a plurality of cell culture cavities, a main flow channel is arranged on the surface of the core chip, and the main flow channel is communicated with the plurality of cell culture cavities. The virus separation and culture method based on the micro-fluidic cell chip adopts a plurality of cell lines for co-culture, realizes the sequential incubation of one sample to be analyzed and different cell lines, can significantly save clinical samples, maximally improves sample utilization, simultaneously reduces the consumption of culture reagents, shortens the separation and identification time, and theoretically realizes the culture of at least ten different cell lines at the same time due to the design of the micro-fluidic cell chip provided by the application, significantly increases the throughput of virus sensitive host cell screening, and realizes the high-throughput, automatic and rapid screening of virus sensitive cell lines.

An apparatus for culturing cells in connection with a fluid is provided. The apparatus includes a bioreactor including a bed for culturing cells, an agitator for circulating the fluid within the bioreactor, and a controller adapted to control the agitator to operate at a first speed during a first predetermined amount of time and a second speed thereafter. A bioreactor having a fixed bed including a coating to promote cell adhesion and growth is also provided. Related methods are also disclosed.