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Chlamydia psittaci is a lethal intracellular bacterial species that may cause endemic avian chlamydiosis, epizootic outbreaks in mammals, and respiratory psittacosis in humans. Potential hosts include feral birds and domesticated poultry, as well as cattle, pigs, sheep, and horses. C. psittaci is transmitted by inhalation, contact, or ingestion among birds and to mammals. Psittacosis in birds and in humans often starts with flu-like symptoms and becomes a life-threatening pneumonia. Many strains remain quiescent in birds until activated by stress. Birds are excellent, highly mobile vectors for the distribution of chlamydia infection, because they feed on, and have access to, the detritus of infected animals of all sorts.
Multiplex detection of lower respiratory tract pathogens under the principle of probe and reagent kit for flow amplification
The application belongs to the field of biological medicine, and particularly relates to a multiplex detection lower respiratory tract pathogen counterflow amplification primer set, probe and kit. The counterflow amplification primer set comprises a Legionella pneumophila primer pair, an adenovirus primer pair and a Chlamydophila psittaci primer pair. The primer sequences in the Legionella pneumophila primer pair, the adenovirus primer pair and the Chlamydophila psittaci primer pair each comprise a target nucleic acid complementary sequence and an interface sequence. The interface sequence is located at the 5' end of the target nucleic acid sequence and is not complementary to the target nucleic acid. The target nucleic acid complementary sequence has a folding sequence, and the folding sequence is complementary to the interface sequence, so that the primer sequence has a curved hairpin structure. A primer probe is designed, and a respiratory tract pathogenic bacteria multiplex detection kit is prepared. The multiplex detection can avoid non-specific amplification and improve the detection sensitivity.
Owner:INNOVITA BIOLOGICAL TECH CO LTD +2