11 results about "Chlamydia psittaci" patented technology

The invention relates to the technical field of biology, in particular to a primer probe combination product for respiratory tract infection detection. A plurality of specific primers and self-quenching probes are integrated in a single reaction system; simultaneous, rapid and rapid detection on 15 respiratory pathogens (including influenza A virus, influenza B virus, respiratory syncytial virus, severe acute respiratory syndrome coronavirus 2, mycoplasma pneumoniae, parainfluenza virus, rhinovirus, enterovirus, coronavirus, adenovirus, metapneumovirus, bordetella pertussis, bocavirus, chlamydia pneumoniae and chlamydia psittaci) is realized. The detection is accurate.

The invention provides a weak-effect PAM-based variable-temperature one-step method detection system, an establishment method thereof and application of the weak-effect PAM-based variable-temperature one-step method detection system in chlamydia psittaci detection, and belongs to the technical field of detection. The sequences of the chlamydia psittaci RPA primer pair are as shown in SEQ ID NO. 1 and SEQ ID NO. 2, and the sequence of the crRNA molecule is as shown in SEQ ID NO. 3. Based on in-depth study on PAM recognition characteristics of AapCas12b protein, the PAM restriction of the AapCas12b protein has remarkable temperature dependence, namely, the restriction is relatively weak under a high-temperature condition and is remarkably enhanced under a low-temperature condition. The important discovery provides a theoretical basis for developing a novel detection method.

The invention relates to a primer set for whole genome sequencing of chlamydia psittaci, the primer set comprises a primer subset p1 and a primer subset p2, the primer subset p1 comprises primers with sequences as shown in SEQ ID NO: 1-240, and the primer subset p2 comprises primers with sequences as shown in SEQ ID NO: 241-480. The primer group disclosed by the invention not only can accurately obtain the whole genome sequence of chlamydia psittaci, but also is adaptive to the sequencing modes of all second-generation and third-generation sequencing platforms.

The invention discloses a chlamydia psittaci cgMLST molecular typing method, and belongs to the field of microbial molecular typing and tracing. According to the invention, an operation technology for carrying out molecular typing on chlamydia psittaci by using a core genome multi-site sequence typing system (cgMLST) is established for the first time. The chlamydia psittaci cgMLST system is established through the processes of collecting chlamydia psittaci genomes in a disclosed genome database, obtaining core genomes through quality control screening and generic genome analysis, screening core genes suitable for typing under multiple screening conditions and the like. And comparing with a phylogenetic analysis result based on core genome SNP difference to confirm the resolution and reliability of the typing system. According to the typing technology, the core genome of the chlamydia psittaci is determined for the first time, and the typing technology can be used for molecular typing and epidemiological monitoring of the chlamydia psittaci.

The invention belongs to the technical field of prevention and control of pigeon chlamydia disease, and particularly relates to pigeon source chlamydia psittaci and application thereof. The invention provides a strain of pigeon-derived chlamydia psittaci, and the collection number of the pigeon-derived chlamydia psittaci is CGMCC (China General Microbiological Culture Collection Center) No. 46418. The invention also provides a vaccine which contains the pigeon source chlamydia psittaci. According to the vaccine, the pigeons inoculated with the vaccine can effectively resist infection of chlamydia psittaci, the relative protection rate of the pigeons is 78.3%, and the vaccine has a good application prospect in the large-scale pigeon breeding industry.

The invention discloses a primer probe combination and application thereof in simultaneous identification of chlamydia psittaci and chlamydia abortus, and relates to the technical field of animal molecular biology test methods and test reagents. The method comprises the following steps: firstly, designing a universal primer probe for a common conserved region of chlamydia parrot (15 genotypes) and chlamydia abortus ompA genes, then designing an identification primer probe for conserved but different regions of ompA genes of two chlamydia, respectively marking FAM and VIC fluorophores by the two probes, then screening out an optimal primer probe combination and optimal PCR amplification reaction conditions, and finally identifying the ompA genes of the chlamydia parrot (15 genotypes) and the ompA genes of the chlamydia abortus. Finally, the sensitivity, specificity, repeatability and the like of the primer probe combination are verified through experiments. By using the detection method disclosed by the invention, the chlamydia psittaci and the chlamydia abortus can be identified at the same time, all genotypes of chlamydia psittaci can be detected, and the effect is obviously superior to that of the existing known detection method.

The invention provides a primer and a probe group for detecting sheep abortion-causing bacteria and application of the primer and the probe group, and belongs to the technical field of biological detection. The primer and probe group is characterized in that the sequence of the primer and probe group for detecting the listeria is shown as SEQ ID NO.1-3, and the sequence of the probe group for detecting the listeria is shown as SEQ ID NO.2-3; according to the primer for detecting the salmonella abortus, the sequence of the probe group is as shown in SEQ ID NO.4-6; the sequences of the primer and the probe group aiming at Brucella detection are shown as SEQ ID NO.7-9; the sequence of a primer and a probe group for detecting chlamydia psittaci is as shown in SEQ ID NO.10-12. The invention further discloses a kit for detecting chlamydia psittaci. The method has the characteristics of visualization, high sensitivity, strong specificity, simplicity and convenience in operation, short time consumption and the like, realizes rapid differential diagnosis of four sheep bacterial abortion diseases through one-time PCR amplification, and has important advantages in the field of sheep disease diagnosis and identification.

The invention belongs to the field of prevention and control of psittaci diseases, and relates to an LAMP primer combination for detecting chlamydia psittaci and application of the LAMP primer combination. The invention provides an LAMP (loop-mediated isothermal amplification) primer combination for detecting chlamydia psittaci, and the LAMP primer combination comprises the following primers: FIP: 5 '-GTACATGTTTCAAGTTTATTGCCGATGCTAACAAATTTCAGAACAATTACAACAACAATTACAACAACGATTCCGC-3', BIP: 5 '-ACTTGGAAGCTATCTGTAGGTAAACAAGGAGA-3', F3: 5 '-GTATCTGCAACGGATCTG-3' and B3: 5 '-TCCTGTTAACGATTCCGC-3'. The primer combination can be used for LAMP detection of chlamydia psittaci, has the advantages of high sensitivity, strong specificity and

The invention discloses a chlamydia psittaci liquid-phase probe targeted capturing and library building method and a kit thereof, and belongs to the technical field of virus detection, a specific probe is designed to enrich pathogen DNA in a targeted manner, a sample treatment process is optimized, targeted enrichment and high-throughput sequencing are combined, and the method has the advantages of wide coverage, good flexibility, high sensitivity, high accuracy and the like. The method has the characteristics of high sensitivity, high specificity and the like, and can be used for quantitative, qualitative and deep analysis and other multi-dimensional information output. By designing the specific probe and optimizing the sample treatment and library building process, the sensitivity, specificity and efficiency of chlamydia psittaci detection are remarkably improved, and the blank in the aspects of low-load detection, whole-genome analysis and high-throughput adaptation in the prior art is filled; the method has important application value in the fields of clinical diagnosis, poultry epidemic disease prevention and control and public health safety.

The invention belongs to the field of biomedical treatment, and particularly relates to a convection amplification primer group, a probe and a kit for multiple detection of lower respiratory pathogens. The convection amplification primer pair comprises a legionella pneumophila primer pair, an adenovirus primer pair and a chlamydia psittaci primer pair, primer sequences in the legionella pneumophila primer pair, the adenovirus primer pair and the chlamydia psittaci primer pair respectively comprise a target nucleic acid complementary sequence and an interface sequence, and the interface sequence is located at the 5'end of the target nucleic acid sequence; the nucleic acid is not complementary with the target nucleic acid; the target nucleic acid complementary sequence has a folded sequence, and the folded sequence is complementary to the interface sequence, so that the primer sequence has a bent hairpin structure. Meanwhile, a primer probe is designed, and a respiratory tract pathogenic bacteria multiple detection kit is prepared. The multiple detection can avoid non-specific amplification and improve the detection sensitivity.

The application belongs to the field of biological medicine, and particularly relates to a multiplex detection lower respiratory tract pathogen counterflow amplification primer set, probe and kit. The counterflow amplification primer set comprises a Legionella pneumophila primer pair, an adenovirus primer pair and a Chlamydophila psittaci primer pair. The primer sequences in the Legionella pneumophila primer pair, the adenovirus primer pair and the Chlamydophila psittaci primer pair each comprise a target nucleic acid complementary sequence and an interface sequence. The interface sequence is located at the 5' end of the target nucleic acid sequence and is not complementary to the target nucleic acid. The target nucleic acid complementary sequence has a folding sequence, and the folding sequence is complementary to the interface sequence, so that the primer sequence has a curved hairpin structure. A primer probe is designed, and a respiratory tract pathogenic bacteria multiplex detection kit is prepared. The multiplex detection can avoid non-specific amplification and improve the detection sensitivity.