30 results about "Chlamydia psittaci" patented technology

The invention relates to a detection kit, and specifically discloses a kit for detecting avian chlamydia psittaci by enzyme linked immunosorbent assay. A genetic engineering expression recombinant protein MOMP is used as an antigen for wrapping a solid-phase carrier for enzyme linked immunosorbent assay adsorption detection. By the adoption of genetic engineering expression high-purity recombinant antigen as a wrapping antigen, the kit is high in specificity and high in sensitivity. After wrapping the solid-phase carrier, the antigen is protected with an antigen protector, so that the storage time of the antigen is prolonged. Furthermore, an enzyme linked antibody used in the kit is horse radish peroxidase-marked rabbit-anti bird IgG and can be widely used for detecting the universality of poultry such as chickens, ducks and gooses.

The present invention relates to a kit for detecting microorganisms, particularly a kit for detection of abortion chlamydia psittaci for pigs, sheep and cattle detection, and a preparation method and a use method thereof. The kit for detection of abortion chlamydia psittaci of the present invention comprises two pairs of specific primers composed of an outer primer and an inner primer, wherein the sequence of an upstream outer primer F3 is SEQ ID No. 1; the sequence of a downstream outer primer B3 is SEQ ID No. 2; the sequence of an upstream inner primer FIP is SEQ ID No. 3; and the sequence of an downstream inner primer BIP is SEQ ID No. 4. The kit of the invention has high sensitivity and high specificity, is simple and convenient in the detection method, and can be applicable to detection of pigs, sheep and cattle.

The invention relates to an inactivated vaccine of cow chlamydia, its preparation and the related inspection method during the vaccine preparation. The preparing process comprises diluting the Chlamydia psittaci SX 5 or NX with microcosmic salt buffering liquid or physiological saline, vaccinating to healthy chick embryo hatched at 37 deg. C for 6-7 days, harvesting vitelline membrane and allantois liquid of dead chick embryo after 72 hours as antigens, triturating the antigens, diluting and charging formaldehyde for deactivation, mixing the deactivated antigens with oil adjuvant by the proportion of 1:1, stirring homogeneously, carrying out homogeneous emulsion to obtain the vaccine.

The invention discloses animal chlamydia TaqMan-MGB probe multiplex real-time fluorescent quantitative PCR (polymerase chain reaction) detection primers, kit and method. The multiplex TaqMan-MGB probe real-time fluorescent PCR method can perform quick detection on Ch. Abortus, Ch.pecorum and Ch.psittaci. On the basis of the conserved region of the three chlamydia target sequences MOMP, the detection method designs three pairs of primers and three TaqMan-MHB probes. The method can quickly, efficiently, specifically and sensitively detect the target sequences by a two-step amplification process under simple reaction conditions, is simple to operate, does not need any expensive instrument or reagent, and has the advantages of no technical requirements for operating personnel, low detection cost and short detection time. The method can avoid cross contamination which can possibly occur due to agarose electrophoresis, thereby enhancing the detection accuracy and reliability.

The invention relates to a bovine abortus Chlamydia psittaci subunit vaccine and a preparation method thereof. The vaccine is the subunit vaccine formed by Chlamydophila abortus outer membrane protein A (OmpA), and the preparation method of the vaccine comprises the steps of extracting bacterial total DNA to be served as a template from the Chlamydophila abortus, designing two pairs of specific primers and conducting nested polymerase chain reaction and amplification to obtain the complete gene of the Chlamydophila abortus outer membrane protein A (OmpA), and cloning the gene to a prokaryotic expression vector to obtain a recombinant expression vector; using the recombinant expression vector to transform colon bacillus competent cells, extracting recombinant plasmid and screening out positive clone by PCR and enzyme cutting identification; and transferring the recombinant plasmid into the colon bacillus and conducting induction expression to obtain the antigen protein used for the vaccine.

The invention provides a traditional Chinese medicine composition for treating chlamydia psittaci disease, which comprises radix scutellariae, rhizoma blechni, fructus mume, fructus forsythiae, rhizoma polygoni cuspidati, rhizoma bletillae, ginseng, rhizoma atractylodis macrocephalae, radix sophorae flavescentis, radix pulsatillae, caulis spatholobi and radix puerariae. The traditional Chinese medicine composition provided by the invention has the advantages of obvious curative effect, high cure rate, rare recurrence and the like in treating the chicken suffering chlamydia psittaci disease.

The invention provides an avian chlamydophila psittaci outer membrane protein N-PmpD, a preparation method and the application. The protein N-PmpD refers to: 1) protein composed by an amino acid sequence showed in SEQ ID No.2, or 2) protein derived from 1) and with equal activity by substituting, missing or adding one or several amino acid in the amino acid sequence showed in SEQ ID No.2. The invention further discloses a gene for coding the protein. Experiments show that the protein N-PmpD has good immunogenicity, can serve as an immunogen to enhance resistance of birds to infection of avian chlamydophila psittaci, and is good in immune effect.

The invention relates to an amplification detection kit, in particular to an LAMP primer and a loop-mediated isothermal amplification detection kit for pets suffering from human-animal comorbid chlamydia psittaci disease. The invention researches and develops a loop-mediated isothermal amplification detection method for the human-animal comorbid chlamydia psittaci disease of pets. According to the method, on the basis of the loop-mediated isothermal amplification principle, universal amplification primers are designed for the chlamydia psittaci ompA gene, a reaction system is constructed by adjusting the ratio of reaction reagents and a primer group, the reaction time is optimized, only a constant-temperature heating instrument or a water bath kettle is needed, professionals are not needed, the result can be judged according to the reaction color development difference in 30 minutes. 9 types of chlamydia psittaci including genotypes A, B, C, D, E, F, E/B, M56 and WC can be detected. The experiment time is greatly shortened, the experiment development conditions are reduced, rapid diagnosis of pet epidemic diseases by grass-roots units is realized, and the purpose of rapid diagnosis of the human-animal comorbid chlamydia is achieved.

The invention relates to construction of a rough Brucella strain of a recombinant chlamydia psittaci outer membrane protein MOMP gene and a vaccine thereof. A rough bovine Brucella attenuated strain RA343 is taken as a parent strain, a sucrose suicide plasmid is taken as a vector, the Chlamydia psittaci outer membrane protein MOMP gene containing a specific promoter sequence is inserted into a Brucella genome without trace after codon optimization, and the recombinant Brucella strain RA343-MOMP capable of efficiently expressing the Chlamydia psittaci outer membrane protein gene is successfullyconstructed. The recombinant strain not only retains the rough type characteristic of an original parent strain RA343 and has good immunoprotection on brucellosis (brucellosis). In addition, an MOMPantibody against chlamydia psittaci can be generated after the recombinant strain is used for immunizing animals, and accordingly immunoprotection on chlamydia psittaci is achieved. The vaccine prepared by the recombinant vaccine strain can simultaneously realize double immune protection on brucellosis and chlamydia psittaci after being used for immunizing animals.

The present invention describes novel methods for the specific detection and identification of Chlamydophila psittaci genotypes. According to one embodiment the method makes use of quantitative PCR with internal probes and optionally competitor probes which increase specificity. The invention also describes a strain of Cp. psittaci with a novel genotype EB and methods to distinguish said novel genotype from previously identified genotypes.

The invention provides a primer for detection of the genotype of chlamydia psittaci, a probe combination and application. According to the allelism and equivalence property between the serotype and ompA genotype of the chlamydia psittaci (C.psittaci), by analyzing and screening gene sequences of VD1-VD4 areas of the ompA genotype of the chlamydia psittaci, a specificity probe and primer of the C.psittaci genetype are designed. The invention further provides a genotype identification chip of an ompA gene specificity area manufactured by the probe. A novel micro-array chip method for quickly andeffectively detecting the C.psittaci genotype with high flux is good in sensibility and specificity, a novel practical and effective detection method is provided for diagnosis, analysis and researchon the genotype of the chlamydia psittaci, and an effective means is provided for diagnosis of the chlamydia psittaci, epidemiological investigation and prevention and control over infectious diseases.

The present invention relates to means and methods to protect against disease caused by bacteria belonging to the genus Chlamydia. In particular, the present invention relates to isolated B- and T-cell epitopes derived from the major outer membrane protein of Chlamydia psittaci which can be used against an infection with a species of the genus Chlamydia. More in particular, the invention provides a vaccine which can be used against chlamydiosis caused by Chlamydia psittaci in birds and man. In addition, the invention relates to a diagnostic method to diagnose the latter infections.

The invention provides a preparation method of an avian chlamydia psittaci polymorphic outer membrane protein (PmpG) protein and an application thereof. The method includes: 1) chlamydia psittaci PmpG protein which comprises primers, vectors, expression conditions and a protein renaturation technology for 17G, 19G, 20G and 21G protein expression are provided; 2) the prepared Pmp20G protein is used as a coating antigen to prepare an ELISA diagnostic kit, the kit has good specificity, sensitivity and repeatability, the coincidence rate of the kit reaches 98.1% compared with a foreign commercial kit, and the kit has no cross reaction with other related respiratory pathogens; 3) the four protein compositions of chlamydia psittaci Pmp17G, 19G, 20G and 21G are used as antigens, and the prepared vaccine has the characteristics of small dosage, high synergistic effect, high immune protection efficacy and the like. and 4) a composition of four proteins of chlamydia psittaci Pmp17G, 19G, 20G and 21G is taken as an antigen, chitosan gel is taken as an adjuvant, and good respiratory mucosa immunity can be generated through an aerial fog immune way, so that infection of the chlamydia psittaci is prevented and treated, and spreading of the chlamydia psittaci from livestock and poultry to people is blocked.

The invention discloses an emodin methyl ether microemulsion and an application thereof. The emodin methyl ether microemulsion is a microemulsion system composed of an organic solvent and a surfactant. Preferably, the formula of the microemulsion system comprises one or more of N, N-dimethylformamide or N, N-dimethylacetamide, ethanol or Tween-20. In-vitro experiments prove that the physcion can be used for killing clinical drug-resistant bacillus cereus, enterococcus faecalis, bacillus gallisepticum, ornithobacterium rhinotracheitis, haemophilus paragallinarum, klebsiella, chlamydia psittaci, mycoplasma gallisepticum and mycoplasma synoviae. Meanwhile, animal experiments prove that the emodin methyl ether microemulsion is suitable for preventing and treating respiratory tract diseases caused by avian rhinotracheitis, haemophilus paragallinarum, klebsiella, chlamydia psittaci and mycoplasma gallisepticum clinically, liver rupture caused by bacillus cereus, enterococcus faecalis and chicken bacilli, and the like. The bovine mastitis and endometritis caused by klebsiella and bacillus cereus can be treated.