30 results about "Chlamydia psittaci" patented technology

The invention provides a primer for detection of the genotype of chlamydia psittaci, a probe combination and application. According to the allelism and equivalence property between the serotype and ompA genotype of the chlamydia psittaci (C.psittaci), by analyzing and screening gene sequences of VD1-VD4 areas of the ompA genotype of the chlamydia psittaci, a specificity probe and primer of the C.psittaci genetype are designed. The invention further provides a genotype identification chip of an ompA gene specificity area manufactured by the probe. A novel micro-array chip method for quickly andeffectively detecting the C.psittaci genotype with high flux is good in sensibility and specificity, a novel practical and effective detection method is provided for diagnosis, analysis and researchon the genotype of the chlamydia psittaci, and an effective means is provided for diagnosis of the chlamydia psittaci, epidemiological investigation and prevention and control over infectious diseases.

The invention relates to a detection kit, and specifically discloses a kit for detecting avian chlamydia psittaci by enzyme linked immunosorbent assay. A genetic engineering expression recombinant protein MOMP is used as an antigen for wrapping a solid-phase carrier for enzyme linked immunosorbent assay adsorption detection. By the adoption of genetic engineering expression high-purity recombinant antigen as a wrapping antigen, the kit is high in specificity and high in sensitivity. After wrapping the solid-phase carrier, the antigen is protected with an antigen protector, so that the storage time of the antigen is prolonged. Furthermore, an enzyme linked antibody used in the kit is horse radish peroxidase-marked rabbit-anti bird IgG and can be widely used for detecting the universality of poultry such as chickens, ducks and gooses.

The invention discloses a coexpression vector of Chlamydophila abortus and Chlamydophila psittaci protective antigen MOMP and MIP, a construction method and an expression method thereof. By means of a molecular cloning technique, the coding genes of Chlamydophila abortus's main outer membrane protein (MOMPa) and macrophage infection potentiator (MIPa) and Chlamydophila psittaci's main outer membrane protein (MOMPp) and macrophage infectivity potentiator (MIPp) are respectively connected to the dual expression vector pETDuet-1 and pRSFDuet-1 so as to construct the coexpression vector. The coexpression vector provided by the invention carries four protein genes of MOMP and MIP of Chlamydophila abortus and Chlamydophila psittaci, can realize expression in one host bacterium, and can be used for preparing polyvalent chlamydial genetic engineering subunit vaccines and preventing and controlling multi-serotype animal infectious diseases, is in favor of improving the technological level for controlling animal chlamydial diseases in China, thus ensuring the sound development of livestock raising industry, increasing the income of farmers and herdsmen, and guaranteeing public health and animal product safety.

The invention relates to the construction of a recombinant brucella brucella of the outer membrane protein MOMP gene of chlamydia psittaci (Cps) and a vaccine thereof. In the present invention, the attenuated Brucella bovis attenuated strain RA343 is used as the parent strain, and the sucrose suicide plasmid is used as the carrier, and the MOMP gene of the outer membrane protein of Chlamydia psittaci containing a specific promoter sequence is seamlessly inserted into the In the Brucella genome, a recombinant Brucella RA343‑MOMP strain capable of highly expressing the outer membrane protein MOMP gene of Chlamydia psittaci was successfully constructed. The recombinant strain not only retains the rough characteristics of the original parent strain RA343, but also has good immune protection against brucellosis (brucellosis), and after the recombinant strain immunizes animals, it can produce MOMP antibodies against Chlamydia psittaci, thereby achieving Immunoprotection against Chlamydia psittaci. The vaccine prepared by using the recombinant vaccine strain can simultaneously realize double immune protection against brucellosis and Chlamydia psittaci after immunizing animals.

The present invention relates to means and methods to protect against disease caused by bacteria belonging to the genus Chlamydia. In particular, the present invention relates to isolated B- and T-cell epitopes derived from the major outer membrane protein of Chlamydia psittaci which can be used against an infection with a species of the genus Chlamydia. More in particular, the invention provides a vaccine which can be used against chlamydiosis caused by Chlamydia psittaci in birds and man. In addition, the invention relates to a diagnostic method to diagnose the latter infections.

The invention discloses animal chlamydia TaqMan-MGB probe multiplex real-time fluorescent quantitative PCR (polymerase chain reaction) detection primers, kit and method. The multiplex TaqMan-MGB probe real-time fluorescent PCR method can perform quick detection on Ch. Abortus, Ch.pecorum and Ch.psittaci. On the basis of the conserved region of the three chlamydia target sequences MOMP, the detection method designs three pairs of primers and three TaqMan-MHB probes. The method can quickly, efficiently, specifically and sensitively detect the target sequences by a two-step amplification process under simple reaction conditions, is simple to operate, does not need any expensive instrument or reagent, and has the advantages of no technical requirements for operating personnel, low detection cost and short detection time. The method can avoid cross contamination which can possibly occur due to agarose electrophoresis, thereby enhancing the detection accuracy and reliability.

The invention discloses a method for detecting infectious disease pathogens possibly existing in a biological sample. The infectious disease pathogens comprise chlamydia psittaci, pneumocystis carinii, leptospira, Q fever rickettsiosis and brucellosis. The method comprises the following steps: expanding nucleic acid fragments of the biological sample, and detecting the nucleic acid fragments by using a probe. The invention also provides primers used for expanding and the probe used for detection. The invention also provides a kit comprising the primers. The method has the advantages of high sensitivity, strong specificity, simple operation and wide sample range, can simultaneously detect various infectious disease pathogens, and is suitable for early diagnosis of respiratory infectious diseases.