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30 results about "Chlamydia psittaci" patented technology
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Chlamydia psittaci is a lethal intracellular bacterial species that may cause endemic avian chlamydiosis, epizootic outbreaks in mammals, and respiratory psittacosis in humans. Potential hosts include feral birds and domesticated poultry, as well as cattle, pigs, sheep, and horses. C. psittaci is transmitted by inhalation, contact, or ingestion among birds and to mammals. Psittacosis in birds and in humans often starts with flu-like symptoms and becomes a life-threatening pneumonia. Many strains remain quiescent in birds until activated by stress. Birds are excellent, highly mobile vectors for the distribution of chlamydia infection, because they feed on, and have access to, the detritus of infected animals of all sorts.
Kit for detecting avian chlamydia psittaci by enzyme linked immunosorbent assay
ActiveCN105606826ALong storage timeStrong specificityBiological material analysisBiological testingAntigenImmuno detection
The invention relates to a detection kit, and specifically discloses a kit for detecting avian chlamydia psittaci by enzyme linked immunosorbent assay. A genetic engineering expression recombinant protein MOMP is used as an antigen for wrapping a solid-phase carrier for enzyme linked immunosorbent assay adsorption detection. By the adoption of genetic engineering expression high-purity recombinant antigen as a wrapping antigen, the kit is high in specificity and high in sensitivity. After wrapping the solid-phase carrier, the antigen is protected with an antigen protector, so that the storage time of the antigen is prolonged. Furthermore, an enzyme linked antibody used in the kit is horse radish peroxidase-marked rabbit-anti bird IgG and can be widely used for detecting the universality of poultry such as chickens, ducks and gooses.
Owner:CHINA AGRI UNIV
Kit for detection of abortion chlamydia psittaci and method
InactiveCN102787162AIncreased sensitivityStrong specificityMicrobiological testing/measurementMicroorganismChlamydia psittaci
The present invention relates to a kit for detecting microorganisms, particularly a kit for detection of abortion chlamydia psittaci for pigs, sheep and cattle detection, and a preparation method and a use method thereof. The kit for detection of abortion chlamydia psittaci of the present invention comprises two pairs of specific primers composed of an outer primer and an inner primer, wherein the sequence of an upstream outer primer F3 is SEQ ID No. 1; the sequence of a downstream outer primer B3 is SEQ ID No. 2; the sequence of an upstream inner primer FIP is SEQ ID No. 3; and the sequence of an downstream inner primer BIP is SEQ ID No. 4. The kit of the invention has high sensitivity and high specificity, is simple and convenient in the detection method, and can be applicable to detection of pigs, sheep and cattle.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Primer group and kit for rapidly identifying respiratory tract microorganisms based on nanopore sequencing and application of primer group
ActiveCN112501268APrecise positioningImprove accuracyMicrobiological testing/measurementAgainst vector-borne diseasesPneumonitisEnterococcus
The invention discloses a primer group for detecting respiratory tract microorganisms based on a nanopore sequencing method. The nucleotide sequences of the primer group are as shown in SEQ ID NO:1-20. The microorganisms are streptococcus pneumoniae, staphylococcus aureus (resisting to methicillin), klebsiella pneumoniae, pseudomonas aeruginosa, acinetobacter baumannii, stenotrophomonas maltophilia, candida albicans, haemophilus influenzae, legionella pneumophila, enterococcus faecium, chlamydia psittaci, cryptococcus gattii, aspergillus fumigatus and pneumocystis jiroveci. According to the invention, sequencing optimization is carried out on different types of samples of the respiratory tract microorganisms, so that the detection method, the kit and the like are suitable for various typesof respiratory tract samples; and a targeted amplification method is adopted, so that the detection requirements of sputum, alveolar lavage and other sample types can be met. In addition, parallel sequencing can be performed, so that the flux of the detected samples is increased, the sequencing time is shortened, and the contradiction between the flux of detected pathogenic species and the cost and time is further relieved.
Owner:GUANGZHOU DARUI BIOTECH
Inactivated vaccine of cow chlamydia, its preparation and inspection method
ActiveCN1698892AFight infectionInfection fromChlamydiaceae ingredientsAntiinfectivesMicrocosmic saltOil adjuvant
The invention relates to an inactivated vaccine of cow chlamydia, its preparation and the related inspection method during the vaccine preparation. The preparing process comprises diluting the Chlamydia psittaci SX 5 or NX with microcosmic salt buffering liquid or physiological saline, vaccinating to healthy chick embryo hatched at 37 deg. C for 6-7 days, harvesting vitelline membrane and allantois liquid of dead chick embryo after 72 hours as antigens, triturating the antigens, diluting and charging formaldehyde for deactivation, mixing the deactivated antigens with oil adjuvant by the proportion of 1:1, stirring homogeneously, carrying out homogeneous emulsion to obtain the vaccine.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Establishment method for Taqman MGB probe real-time fluorescence quantitative PCR detection system taking outer membrane protein as target gene
InactiveCN101812504AMicrobiological testing/measurementMicroorganism based processesFluorescenceGel electrophoresis
The invention relates to a method for rapid detection and diagnosis of Chlamydophila psittaci by using real-time PCR which is one of emerging molecular biotechnology. Based on the conserved domain of the variable domain III (VD3) of the main outer membrane protein (MOMP) gene of the Chlamydophila psittaci, the detection system of the invention designs a pair of primers and a relevant Taqman MGB probe. The system can detect Chlamydophila psittaci genes existing in bird dung and the sensitivity of the system is obviously higher than the sensitivity of common PCR. Since the Real-time PCR requires no post-PCR processing, the cross contamination possibly caused by agarose gel electrophoresis is avoided and thereby the accuracy and the credibility of the detection are improved.
Owner:KUNMING UNIV OF SCI & TECH
Animal chlamydia TaqMan-MGB probe multiplex real-time fluorescent quantitative PCR (polymerase chain reaction) detection primers, kit and method
ActiveCN103555842AStrong specificityHigh sensitivityMicrobiological testing/measurementFluorescence/phosphorescenceAgarose electrophoresisInverse polymerase chain reaction
The invention discloses animal chlamydia TaqMan-MGB probe multiplex real-time fluorescent quantitative PCR (polymerase chain reaction) detection primers, kit and method. The multiplex TaqMan-MGB probe real-time fluorescent PCR method can perform quick detection on Ch. Abortus, Ch.pecorum and Ch.psittaci. On the basis of the conserved region of the three chlamydia target sequences MOMP, the detection method designs three pairs of primers and three TaqMan-MHB probes. The method can quickly, efficiently, specifically and sensitively detect the target sequences by a two-step amplification process under simple reaction conditions, is simple to operate, does not need any expensive instrument or reagent, and has the advantages of no technical requirements for operating personnel, low detection cost and short detection time. The method can avoid cross contamination which can possibly occur due to agarose electrophoresis, thereby enhancing the detection accuracy and reliability.
Owner:重庆海关技术中心
Abortus Chlamydia psittaci vaccine and preparation method thereof
InactiveCN101579521AHigh expressionRealize large-scale productionChlamydiaceae ingredientsMicroorganism based processesBacteroidesAntigen
The invention relates to a bovine abortus Chlamydia psittaci subunit vaccine and a preparation method thereof. The vaccine is the subunit vaccine formed by Chlamydophila abortus outer membrane protein A (OmpA), and the preparation method of the vaccine comprises the steps of extracting bacterial total DNA to be served as a template from the Chlamydophila abortus, designing two pairs of specific primers and conducting nested polymerase chain reaction and amplification to obtain the complete gene of the Chlamydophila abortus outer membrane protein A (OmpA), and cloning the gene to a prokaryotic expression vector to obtain a recombinant expression vector; using the recombinant expression vector to transform colon bacillus competent cells, extracting recombinant plasmid and screening out positive clone by PCR and enzyme cutting identification; and transferring the recombinant plasmid into the colon bacillus and conducting induction expression to obtain the antigen protein used for the vaccine.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Traditional Chinese medicine composition for treating chlamydia psittaci disease
InactiveCN102988760ASignificant effectHigh cure rateAntibacterial agentsPlant ingredientsDiseaseCure rate
The invention provides a traditional Chinese medicine composition for treating chlamydia psittaci disease, which comprises radix scutellariae, rhizoma blechni, fructus mume, fructus forsythiae, rhizoma polygoni cuspidati, rhizoma bletillae, ginseng, rhizoma atractylodis macrocephalae, radix sophorae flavescentis, radix pulsatillae, caulis spatholobi and radix puerariae. The traditional Chinese medicine composition provided by the invention has the advantages of obvious curative effect, high cure rate, rare recurrence and the like in treating the chicken suffering chlamydia psittaci disease.
Owner:张峰
Avian chlamydophila psittaci outer membrane protein N-PmpD, preparation method and application
The invention provides an avian chlamydophila psittaci outer membrane protein N-PmpD, a preparation method and the application. The protein N-PmpD refers to: 1) protein composed by an amino acid sequence showed in SEQ ID No.2, or 2) protein derived from 1) and with equal activity by substituting, missing or adding one or several amino acid in the amino acid sequence showed in SEQ ID No.2. The invention further discloses a gene for coding the protein. Experiments show that the protein N-PmpD has good immunogenicity, can serve as an immunogen to enhance resistance of birds to infection of avian chlamydophila psittaci, and is good in immune effect.
Owner:CHINA AGRI UNIV
Method for detecting infectious disease pathogens and kit
ActiveCN101545009AThe detection process is fastImprove efficiencyMicrobiological testing/measurementMicroorganism based processesQ feverChlamydia psittaci
The invention discloses a method for detecting infectious disease pathogens possibly existing in a biological sample. The infectious disease pathogens comprise chlamydia psittaci, pneumocystis carinii, leptospira, Q fever rickettsiosis and brucellosis. The method comprises the following steps: expanding nucleic acid fragments of the biological sample, and detecting the nucleic acid fragments by using a probe. The invention also provides primers used for expanding and the probe used for detection. The invention also provides a kit comprising the primers. The method has the advantages of high sensitivity, strong specificity, simple operation and wide sample range, can simultaneously detect various infectious disease pathogens, and is suitable for early diagnosis of respiratory infectious diseases.
Owner:海康生物科技(北京)有限公司
LAMP primer and loop-mediated isothermal amplification detection kit for pets suffering from human-animal comorbid chlamydia psittaci disease
ActiveCN113136445AShorten the timeSimple and fast operationMicrobiological testing/measurementDNA/RNA fragmentationDiseaseGenotype
The invention relates to an amplification detection kit, in particular to an LAMP primer and a loop-mediated isothermal amplification detection kit for pets suffering from human-animal comorbid chlamydia psittaci disease. The invention researches and develops a loop-mediated isothermal amplification detection method for the human-animal comorbid chlamydia psittaci disease of pets. According to the method, on the basis of the loop-mediated isothermal amplification principle, universal amplification primers are designed for the chlamydia psittaci ompA gene, a reaction system is constructed by adjusting the ratio of reaction reagents and a primer group, the reaction time is optimized, only a constant-temperature heating instrument or a water bath kettle is needed, professionals are not needed, the result can be judged according to the reaction color development difference in 30 minutes. 9 types of chlamydia psittaci including genotypes A, B, C, D, E, F, E / B, M56 and WC can be detected. The experiment time is greatly shortened, the experiment development conditions are reduced, rapid diagnosis of pet epidemic diseases by grass-roots units is realized, and the purpose of rapid diagnosis of the human-animal comorbid chlamydia is achieved.
Owner:北京市朝阳区动植物疫病预防控制中心(北京市朝阳区农产品质量安全综合检测站)
Rough Brucella for recombinant chlamydia psittaci outer membrane protein MOMP gene and vaccine production method thereof
ActiveCN109939225AProtectImprove weaknessesAntibacterial agentsBacterial antigen ingredientsVaccine ProductionRecombinant vaccines
The invention relates to construction of a rough Brucella strain of a recombinant chlamydia psittaci outer membrane protein MOMP gene and a vaccine thereof. A rough bovine Brucella attenuated strain RA343 is taken as a parent strain, a sucrose suicide plasmid is taken as a vector, the Chlamydia psittaci outer membrane protein MOMP gene containing a specific promoter sequence is inserted into a Brucella genome without trace after codon optimization, and the recombinant Brucella strain RA343-MOMP capable of efficiently expressing the Chlamydia psittaci outer membrane protein gene is successfullyconstructed. The recombinant strain not only retains the rough type characteristic of an original parent strain RA343 and has good immunoprotection on brucellosis (brucellosis). In addition, an MOMPantibody against chlamydia psittaci can be generated after the recombinant strain is used for immunizing animals, and accordingly immunoprotection on chlamydia psittaci is achieved. The vaccine prepared by the recombinant vaccine strain can simultaneously realize double immune protection on brucellosis and chlamydia psittaci after being used for immunizing animals.
Owner:CHINA INST OF VETERINARY DRUG CONTROL
Genotype specific detection of Chlamydophila psittaci
InactiveUS20060008828A1Simple transport and storage requirementEasy sample collectionMicrobiological testing/measurementSpecific detectionGenotype
The present invention describes novel methods for the specific detection and identification of Chlamydophila psittaci genotypes. According to one embodiment the method makes use of quantitative PCR with internal probes and optionally competitor probes which increase specificity. The invention also describes a strain of Cp. psittaci with a novel genotype EB and methods to distinguish said novel genotype from previously identified genotypes.
Owner:UNIV GENT
Coexpression vector of Chlamydophila abortus and Chlamydophila psittaci protective antigen MOMP and MIP, construction and expression method thereof
ActiveCN104131020AGuarantee safe and effectiveAchieve quality controlChlamydiaceae ingredientsDepsipeptidesProtective antigenControl animal
The invention discloses a coexpression vector of Chlamydophila abortus and Chlamydophila psittaci protective antigen MOMP and MIP, a construction method and an expression method thereof. By means of a molecular cloning technique, the coding genes of Chlamydophila abortus's main outer membrane protein (MOMPa) and macrophage infection potentiator (MIPa) and Chlamydophila psittaci's main outer membrane protein (MOMPp) and macrophage infectivity potentiator (MIPp) are respectively connected to the dual expression vector pETDuet-1 and pRSFDuet-1 so as to construct the coexpression vector. The coexpression vector provided by the invention carries four protein genes of MOMP and MIP of Chlamydophila abortus and Chlamydophila psittaci, can realize expression in one host bacterium, and can be used for preparing polyvalent chlamydial genetic engineering subunit vaccines and preventing and controlling multi-serotype animal infectious diseases, is in favor of improving the technological level for controlling animal chlamydial diseases in China, thus ensuring the sound development of livestock raising industry, increasing the income of farmers and herdsmen, and guaranteeing public health and animal product safety.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Primer, kit and method for detecting swine chlamydiosis
InactiveCN105755130AEasy to detectQuick checkMicrobiological testing/measurementMicroorganism based processesElectrophoresisChlamydia psittaci
The invention discloses a primer, a kit and a method for detecting swine chlamydiosis. The primer is shown in SEQ ID No:1 and SEQ ID No.2, and the method comprises the step of carrying out PCR amplification reaction and electrophoretic analysis by taking a to-be-detected pig sample genome DNA as a template. The primer for detecting the swine chlamydiosis can specifically amplify chlamydia psittaci and is unresponsive to other common pathogens; the method has high sensitivity and can conveniently and quickly detect the chlamydia psittaci of various clinical pig samples, specific prevention and control measures can be beneficially rapidly taken, and operation is simple and practical.
Owner:WENS FOOD GRP CO LTD
Primer for detection of genotype of chlamydia psittaci, probe combination and application
ActiveCN109988853AAccurate detectionQuick checkMicrobiological testing/measurementMicroorganism based processesOmpa geneSerotype
The invention provides a primer for detection of the genotype of chlamydia psittaci, a probe combination and application. According to the allelism and equivalence property between the serotype and ompA genotype of the chlamydia psittaci (C.psittaci), by analyzing and screening gene sequences of VD1-VD4 areas of the ompA genotype of the chlamydia psittaci, a specificity probe and primer of the C.psittaci genetype are designed. The invention further provides a genotype identification chip of an ompA gene specificity area manufactured by the probe. A novel micro-array chip method for quickly andeffectively detecting the C.psittaci genotype with high flux is good in sensibility and specificity, a novel practical and effective detection method is provided for diagnosis, analysis and researchon the genotype of the chlamydia psittaci, and an effective means is provided for diagnosis of the chlamydia psittaci, epidemiological investigation and prevention and control over infectious diseases.
Owner:CHINA AGRI UNIV
Primer composition for detecting or assisting in detecting chlamydia psittaci and application of primer composition
ActiveCN114540525ALow reaction temperatureEasy to carryMicrobiological testing/measurementMicroorganism based processesMicroorganismNucleotide
The invention discloses a composition for detecting or assisting in detecting chlamydia psittaci and application of the composition, and belongs to the field of compositions used in a detection method containing nucleic acid or microorganisms. The technical problem to be solved by the invention is how to detect or assist in detecting chlamydia psittaci with high sensitivity and / or high specificity and / or quickly / simply. The invention provides a primer composition for detecting or assisting in detecting chlamydia psittaci. The primer composition is composed of single-stranded DNA molecules with nucleotide sequences as shown in sequences 2 and 3 in a sequence table. When the composition for detecting or assisting in detecting chlamydia psittaci is used for detecting the chlamydia psittaci through RAA amplification, rapidness and high efficiency are achieved, the amplification reaction can be completed within 15 minutes, and a large number of amplification reactions can be completed only at the constant temperature; the detection result is good in specificity and high in sensitivity; the identification method is simple and convenient, and is suitable for rapid detection of on-site or clinical specimens.
Owner:ACADEMY OF MILITARY MEDICAL SCI
Vaccines for chlamydia
The present invention relates to means and methods to protect against disease caused by bacteria belonging to the genus Chlamydia. In particular, the present invention relates to isolated B- and T-cell epitopes derived from the major outer membrane protein of Chlamydia psittaci which can be used against an infection with a species of the genus Chlamydia. More in particular, the invention provides a vaccine which can be used against chlamydiosis caused by Chlamydia psittaci in birds and man. In addition, the invention relates to a diagnostic method to diagnose the latter infections.
Owner:UNIV GENT
Primer and probe combination and application for Chlamydia psittacosis genotype detection
ActiveCN109988853BAccurate detectionQuick checkMicrobiological testing/measurementMicroorganism based processesInfectious DisorderSerotype
Owner:CHINA AGRI UNIV
Preparation method and application of chlamydia psittaci polytype outer membrane protein PmpG protein
ActiveCN113087778AImprove immune efficiencyImprove securityAntibacterial agentsChlamydiaceae ingredientsChlamydiaeProtein Renaturation
The invention provides a preparation method of an avian chlamydia psittaci polymorphic outer membrane protein (PmpG) protein and an application thereof. The method includes: 1) chlamydia psittaci PmpG protein which comprises primers, vectors, expression conditions and a protein renaturation technology for 17G, 19G, 20G and 21G protein expression are provided; 2) the prepared Pmp20G protein is used as a coating antigen to prepare an ELISA diagnostic kit, the kit has good specificity, sensitivity and repeatability, the coincidence rate of the kit reaches 98.1% compared with a foreign commercial kit, and the kit has no cross reaction with other related respiratory pathogens; 3) the four protein compositions of chlamydia psittaci Pmp17G, 19G, 20G and 21G are used as antigens, and the prepared vaccine has the characteristics of small dosage, high synergistic effect, high immune protection efficacy and the like. and 4) a composition of four proteins of chlamydia psittaci Pmp17G, 19G, 20G and 21G is taken as an antigen, chitosan gel is taken as an adjuvant, and good respiratory mucosa immunity can be generated through an aerial fog immune way, so that infection of the chlamydia psittaci is prevented and treated, and spreading of the chlamydia psittaci from livestock and poultry to people is blocked.
Owner:CHINA AGRI UNIV
An enzyme-linked immunosorbent assay kit for the detection of Chlamydia psittaci
ActiveCN105606826BLong storage timeStrong specificityBiological material analysisBiological testingAntigenFowl
The invention relates to a detection kit, and specifically discloses a kit for detecting avian chlamydia psittaci by enzyme linked immunosorbent assay. A genetic engineering expression recombinant protein MOMP is used as an antigen for wrapping a solid-phase carrier for enzyme linked immunosorbent assay adsorption detection. By the adoption of genetic engineering expression high-purity recombinant antigen as a wrapping antigen, the kit is high in specificity and high in sensitivity. After wrapping the solid-phase carrier, the antigen is protected with an antigen protector, so that the storage time of the antigen is prolonged. Furthermore, an enzyme linked antibody used in the kit is horse radish peroxidase-marked rabbit-anti bird IgG and can be widely used for detecting the universality of poultry such as chickens, ducks and gooses.
Owner:CHINA AGRI UNIV
Preparation method and application of chlamydophila psittaci recombinant protein GST-CPSIT_p7
InactiveCN104975037AIncreased sensitivityHigh concentration of target gene fragmentsBiological testingFermentationChlamydia psittaciChlamydophila psittaci infection
The invention relates to the technical field of genetic engineering, in particular to a preparation method and application of chlamydophila psittaci recombinant protein GST-CPSIT_p7. The method comprises the following steps: step I: constructing recombinant expression plasmid pGEX-6P-1 / CPSIT_p7; step II: inducing the recombinant expression plasmid pGEX-6P-1 / CPSIT_p7 to express; and step III: preparing the recombinant protein GST-CPSIT_p7. By adopting the method, the chlamydophila psittaci recombinant protein GST-CPSIT_p7 is successfully cloned, expressed and purified, and the prepared protein has better immunogenicity. The recombinant protein GST-CPSIT_p7 can be applied to kits used for detecting the chlamydophila psittaci infection, and has the advantages of high specificity, high sensitivity and simplicity and convenience in operation, and clinical diagnosis needs of chlamydophila psittaci infection are met better.
Owner:NANHUA UNIV
Co-expression vector of protective antigens momp and mip of Chlamydia abortus and Chlamydia psittaci and its construction and expression method
ActiveCN104131020BGuarantee safe and effectiveAchieve quality controlChlamydiaceae ingredientsDepsipeptidesAgricultural scienceChlamydophila
The invention discloses a coexpression vector of Chlamydophila abortus and Chlamydophila psittaci protective antigen MOMP and MIP, a construction method and an expression method thereof. By means of a molecular cloning technique, the coding genes of Chlamydophila abortus's main outer membrane protein (MOMPa) and macrophage infection potentiator (MIPa) and Chlamydophila psittaci's main outer membrane protein (MOMPp) and macrophage infectivity potentiator (MIPp) are respectively connected to the dual expression vector pETDuet-1 and pRSFDuet-1 so as to construct the coexpression vector. The coexpression vector provided by the invention carries four protein genes of MOMP and MIP of Chlamydophila abortus and Chlamydophila psittaci, can realize expression in one host bacterium, and can be used for preparing polyvalent chlamydial genetic engineering subunit vaccines and preventing and controlling multi-serotype animal infectious diseases, is in favor of improving the technological level for controlling animal chlamydial diseases in China, thus ensuring the sound development of livestock raising industry, increasing the income of farmers and herdsmen, and guaranteeing public health and animal product safety.
Owner:LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
Brucella crassa of a recombinant Chlamydia psittaci outer membrane protein MOMP gene and its vaccine production method
ActiveCN109939225BProtectImprove weaknessesAntibacterial agentsBacterial antigen ingredientsDiseaseVaccine Production
Owner:CHINA INST OF VETERINARY DRUG CONTROL
Emodin methyl ether microemulsion and application thereof
PendingCN114831937AHelp healthy developmentAntibacterial agentsOrganic active ingredientsBiotechnologyRespiratory tract disease
The invention discloses an emodin methyl ether microemulsion and an application thereof. The emodin methyl ether microemulsion is a microemulsion system composed of an organic solvent and a surfactant. Preferably, the formula of the microemulsion system comprises one or more of N, N-dimethylformamide or N, N-dimethylacetamide, ethanol or Tween-20. In-vitro experiments prove that the physcion can be used for killing clinical drug-resistant bacillus cereus, enterococcus faecalis, bacillus gallisepticum, ornithobacterium rhinotracheitis, haemophilus paragallinarum, klebsiella, chlamydia psittaci, mycoplasma gallisepticum and mycoplasma synoviae. Meanwhile, animal experiments prove that the emodin methyl ether microemulsion is suitable for preventing and treating respiratory tract diseases caused by avian rhinotracheitis, haemophilus paragallinarum, klebsiella, chlamydia psittaci and mycoplasma gallisepticum clinically, liver rupture caused by bacillus cereus, enterococcus faecalis and chicken bacilli, and the like. The bovine mastitis and endometritis caused by klebsiella and bacillus cereus can be treated.
Owner:CHINA AGRI UNIV
A kind of primer composition for detecting or assisting in detecting Chlamydia psittaci and its application
ActiveCN114540525BLow reaction temperatureEasy to carryMicrobiological testing/measurementMicroorganism based processesMicroorganismNucleotide
The invention discloses a composition for detecting or assisting the detection of Chlamydia psittaci and its application, and belongs to the field of compositions used in detection methods comprising nucleic acids or microorganisms. The technical problem to be solved by the present invention is how to detect or assist the detection of Chlamydia psittaci with high sensitivity and / or high specificity and / or fast / or simple. The present invention provides a primer composition for detecting or assisting the detection of Chlamydia psittaci, the primer composition is composed of single-stranded DNA molecules whose nucleotide sequences are respectively shown in Sequences 2 and 3 of the Sequence Listing. Using the composition of the present invention for detection or auxiliary detection of Chlamydia psittacosis with RAA amplification to detect Chlamydia psittacosis is fast and efficient, and the amplification reaction can be completed within 15 minutes, and only a large amount of amplification reaction can be completed at a constant temperature The detection results have good specificity and high sensitivity; the identification method is simple and convenient, and is suitable for rapid detection of field or clinical specimens.
Owner:ACADEMY OF MILITARY MEDICAL SCI
Traditional Chinese medicine composition for treating chlamydia psittaci disease
InactiveCN102988760BSignificant effectHigh cure rateAntibacterial agentsPlant ingredientsCure rateCurative effect
The invention provides a traditional Chinese medicine composition for treating chlamydia psittaci disease, which comprises radix scutellariae, rhizoma blechni, fructus mume, fructus forsythiae, rhizoma polygoni cuspidati, rhizoma bletillae, ginseng, rhizoma atractylodis macrocephalae, radix sophorae flavescentis, radix pulsatillae, caulis spatholobi and radix puerariae. The traditional Chinese medicine composition provided by the invention has the advantages of obvious curative effect, high cure rate, rare recurrence and the like in treating the chicken suffering chlamydia psittaci disease.
Owner:张峰
Vaccines for Chlamydia
The present invention relates to means and methods to protect against disease caused by bacteria belonging to the genus Chlamydia. In particular, the present invention relates to isolated B- and T-cell epitopes derived from the major outer membrane protein of Chlamydia psittaci which can be used against an infection with a species of the genus Chlamydia. More in particular, the invention provides a vaccine which can be used against chlamydiosis caused by Chlamydia psittaci in birds and man. In addition, the invention relates to a diagnostic method to diagnose the latter infections.
Owner:UNIV GENT
Animal chlamydia TaqMan-MGB probe multiplex real-time fluorescent quantitative PCR (polymerase chain reaction) detection primers, kit and method
ActiveCN103555842BHigh sensitivityStrong specificityMicrobiological testing/measurementFluorescence/phosphorescenceAgarose electrophoresisInverse polymerase chain reaction
The invention discloses animal chlamydia TaqMan-MGB probe multiplex real-time fluorescent quantitative PCR (polymerase chain reaction) detection primers, kit and method. The multiplex TaqMan-MGB probe real-time fluorescent PCR method can perform quick detection on Ch. Abortus, Ch.pecorum and Ch.psittaci. On the basis of the conserved region of the three chlamydia target sequences MOMP, the detection method designs three pairs of primers and three TaqMan-MHB probes. The method can quickly, efficiently, specifically and sensitively detect the target sequences by a two-step amplification process under simple reaction conditions, is simple to operate, does not need any expensive instrument or reagent, and has the advantages of no technical requirements for operating personnel, low detection cost and short detection time. The method can avoid cross contamination which can possibly occur due to agarose electrophoresis, thereby enhancing the detection accuracy and reliability.
Owner:重庆海关技术中心
Method for detecting infectious disease pathogens and kit
ActiveCN101545009BThe detection process is fastImprove efficiencyMicrobiological testing/measurementMicroorganism based processesQ feverChlamydia psittaci
The invention discloses a method for detecting infectious disease pathogens possibly existing in a biological sample. The infectious disease pathogens comprise chlamydia psittaci, pneumocystis carinii, leptospira, Q fever rickettsiosis and brucellosis. The method comprises the following steps: expanding nucleic acid fragments of the biological sample, and detecting the nucleic acid fragments by using a probe. The invention also provides primers used for expanding and the probe used for detection. The invention also provides a kit comprising the primers. The method has the advantages of high sensitivity, strong specificity, simple operation and wide sample range, can simultaneously detect various infectious disease pathogens, and is suitable for early diagnosis of respiratory infectious diseases.
Owner:海康生物科技(北京)有限公司
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