The invention relates to a recombinant protein extraction and purification method, comprising engineering bacteria fermentation, engineering bacteria fragmentation, inclusion body washing, recombinant protein denaturation and purification, second recombinant protein denaturation and purification, denaturized recombinant protein renaturation, renaturated recombinant protein placing, ion exchange chromatography and molecular sieve chromatography, wherein, the renaturation recombinant protein is placed for 5 to 8 days under the temperature of 2 to 8 DEG C. The recombinant protein extraction and purification method improves the total yield of the recombinant protein, correspondingly improves the purity and efficiency of the final products, reduces the production cost and is beneficial to large scale industrial production.